Information on EC 3.6.1.15 - nucleoside-triphosphatase:
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EC NUMBERCOMMENTARY
3.6.1.15-

RECOMMENDED NAMEGeneOntology No.
nucleoside-triphosphataseGO:0017111

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
NTP + H2O = NDP + phosphate
show the reaction diagram		----

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
hydrolysis of phosphoric ester----

PATHWAYKEGG LinkMetaCyc Link
Metabolic pathways01100 -
NIL-APYRASE-RXN , RXN-12199 , RXN-14024 , RXN-14201 , RXN-12200
Purine metabolism00230 -
pyrimidine deoxyribonucleotides biosynthesis from CTP-PWY-7210
pyrimidine deoxyribonucleotides de novo biosynthesis I-PWY-7184
pyrimidine deoxyribonucleotides de novo biosynthesis III-PWY-6545
pyrimidine deoxyribonucleotides de novo biosynthesis IV-PWY-7198
Thiamine metabolism00730 -
UTP and CTP dephosphorylation I-PWY-7185

SYSTEMATIC NAMEIUBMB Comments
unspecific diphosphate phosphohydrolaseAlso hydrolyses other nucleoside triphosphates, diphosphates, thiamine diphosphate and FAD.

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
ankyrin repeat-rich membrane spanning proteinHomo sapiens, Rattus norvegicus--668745
DEAH-box Prp22 proteinSaccharomyces cerevisiae--667694
DEAH-box splicing factor Prp22Saccharomyces cerevisiae--667694
DR_0079Deinococcus radioduransQ9RXX6-696275
germ-cell-specific leucine-rich repeat proteinMus musculus--667903
GP086L protein----
HCR-NTPaseHomo sapiens--688347
human cancer-related nucleoside triphosphataseHomo sapiens--688347
KAP NTPaseAgrobacterium tumefaciens, Anabaena sp., Caenorhabditis elegans, Clostridium perfringens, Corynebacterium efficiens, Corynebacterium glutamicum, Cupriavidus metallidurans, Deinococcus radiodurans, Drosophila melanogaster, Escherichia coli, Geobacter sulfurreducens, Homo sapiens, Klebsiella pneumoniae, Leuconostoc mesenteroides, Magnetococcus sp., Magnetococcus sp. mc-1, Nostoc punctiforme, Pseudomonas putida, Rattus norvegicus, Saccharophagus degradans, Staphylococcus epidermidis, Synechocystis sp., Thermotoga maritima, Vibrio parahaemolyticus, Vibrio vulnificus--668745
Kidins220Homo sapiens, Rattus norvegicus--668745
kinase D interacting substance of 220 kDaHomo sapiens, Rattus norvegicus--668745
M86L protein----
NALP14Mus musculus--667903
non-specific nucleoside triphosphataseHomo sapiens--688347
nonstructural protein 13Human coronavirus 229E--670074
NS3Hepatitis C virus--667226
NS3Dengue virus--670994
NS3 proteinClassical swine fever virus--684774
NS3FLClassical swine fever virus--684774
nsp13Human coronavirus 229E--670074
NTPase----
NTPaseSaccharomyces cerevisiae--667694
NTPasepotato virus A, potato virus X--668616
NTPaseAgrobacterium tumefaciens, Anabaena sp., Caenorhabditis elegans, Clostridium perfringens, Corynebacterium efficiens, Corynebacterium glutamicum, Cupriavidus metallidurans, Deinococcus radiodurans, Drosophila melanogaster, Escherichia coli, Geobacter sulfurreducens, Geobacter sulfurreducens DL1, Geobacter sulfurreducens DL-1, Homo sapiens, Klebsiella pneumoniae, Leuconostoc mesenteroides, Magnetococcus sp., Magnetococcus sp. mc-1, Nostoc punctiforme, Pseudomonas putida, Saccharophagus degradans, Saccharophagus degradans 2-40T, Staphylococcus epidermidis, Synechocystis sp., Thermotoga maritima, Vibrio parahaemolyticus, Vibrio vulnificus--668745
NTPaseHuman coronavirus 229E--670074
NTPaseRattus norvegicus--668745, 671005
NTPaseClassical swine fever virus--684774
NTPaseNeospora caninumC9K7J7-718494
NTPase-IToxoplasma gondiiQ27893-718494
NTPase/helicaseDengue virus, Hepatitis C virus, Japanese encephalitis virus, Japanese encephalitis virus JaOH0566, Japanese encephalitis virus JEV--667226
NTPase/helicaseWest Nile virus--656466, 667226
nucleic acid-independent nucleoside triphosphataseSouthampton virusQ04544-656663
nucleoside 5-triphosphatase----
nucleoside triphosphataseBacillus subtilis--669092
nucleoside triphosphataseRattus norvegicus--671005
nucleoside triphosphataseBos taurus--696153
nucleoside triphosphatase (NTPase)/helicaseWest Nile virus--656664
nucleoside triphosphate hydrolaseNeospora caninumC9K7J7-718494
nucleoside triphosphate hydrolaseToxoplasma gondiiQ27893-718494
nucleoside triphosphate phosphohydrolase----
nucleoside-5-triphosphate phosphohydrolase----
nucleotide 5'-triphosphataseDengue virus--670994
P-loop NTPaseAgrobacterium tumefaciens, Anabaena sp., Caenorhabditis elegans, Clostridium perfringens, Corynebacterium efficiens, Corynebacterium glutamicum, Cupriavidus metallidurans, Deinococcus radiodurans, Drosophila melanogaster, Escherichia coli, Geobacter sulfurreducens, Homo sapiens, Klebsiella pneumoniae, Leuconostoc mesenteroides, Magnetococcus sp., Magnetococcus sp. mc-1, Nostoc punctiforme, Pseudomonas putida, Rattus norvegicus, Saccharophagus degradans, Saccharophagus degradans 2-40T, Staphylococcus epidermidis, Synechocystis sp., Thermotoga maritima, Vibrio parahaemolyticus, Vibrio vulnificus--668745
phosphatase, nucleoside tri-----
PilBPseudomonas aeruginosa--688871
PilTPseudomonas aeruginosa--688871
PilUPseudomonas aeruginosa--688871
polypeptide p41Southampton virusQ04544-656663
protein NS3Dengue virus--670994
PVA coat proteinpotato virus A--668616
PVA CPpotato virus A--668616
PVx coat proteinpotato virus X--668616
PVX CPpotato virus X--668616
Rad54Sulfolobus solfataricus--694432
Rad54 homologueSulfolobus solfataricus--694432
Rad54cdSulfolobus solfataricus--694432
Rad55BSulfolobus tokodaii--695127
SHVp41Southampton virusQ04544-656663
Sso0909Sulfolobus solfataricusQ97ZJ7-690865
T4P motor proteinPseudomonas aeruginosa--688871
type IV pilus motor proteinPseudomonas aeruginosa--688871
YtkDBacillus subtilis--669092
MC100R----
additional informationMus musculus-the protein is a member of the LRR-replete NACHT nucleoside triphosphatase family667903
additional informationAgrobacterium tumefaciens, Anabaena sp., Caenorhabditis elegans, Clostridium perfringens, Corynebacterium efficiens, Corynebacterium glutamicum, Cupriavidus metallidurans, Deinococcus radiodurans, Drosophila melanogaster, Escherichia coli, Geobacter sulfurreducens DL-1, Geobacter sulfurreducens DL1, Geobacter sulfurreducens, Homo sapiens, Klebsiella pneumoniae, Leuconostoc mesenteroides, Magnetococcus sp. mc-1, Magnetococcus sp., Nostoc punctiforme, Pseudomonas putida, Rattus norvegicus, Saccharophagus degradans 2-40T, Saccharophagus degradans, Staphylococcus epidermidis, Synechocystis sp., Thermotoga maritima, Vibrio parahaemolyticus, Vibrio vulnificus-the enzyme belongs to the KAP family of NTPases668745
additional informationBacillus subtilis-the enzyme is a member of the Nudix hydrolase superfamily669092
additional informationClassical swine fever virus-the enzyme is a nonstructural protein 3, NS3 of family Flaviviridae members684774
additional informationPseudomonas aeruginosa-PilB and PilT are bipolar proteins belonging to the secretion NTPase superfamily688871
additional informationSulfolobus solfataricusQ97ZJ7Sso0909 is an AAA protein690865
additional informationSulfolobus solfataricus-the enzyme belongs to the the Swi2/Snf2 superfamily694432

CAS REGISTRY NUMBERCOMMENTARY
9075-51-8-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Agrobacterium tumefaciens-668745--Manually annotated by BRENDA team
Anabaena sp.PCC 7120668745--Manually annotated by BRENDA team
Bacillus subtilisstrain 168, gene ytkD669092--Manually annotated by BRENDA team
Bacillus subtilis 168strain 168, gene ytkD669092--Manually annotated by BRENDA team
Bos taurus-696153--Manually annotated by BRENDA team
Caenorhabditis elegans-668745--Manually annotated by BRENDA team
Classical swine fever virusCSFV684774--Manually annotated by BRENDA team
Classical swine fever virus CSFVCSFV684774--Manually annotated by BRENDA team
Clostridium perfringens-668745--Manually annotated by BRENDA team
Corynebacterium efficiens-668745--Manually annotated by BRENDA team
Corynebacterium glutamicum-668745--Manually annotated by BRENDA team
Cupriavidus metallidurans-668745--Manually annotated by BRENDA team
Deinococcus radiodurans-668745--Manually annotated by BRENDA team
Deinococcus radiodurans-696275Q9RXX6SwissProtManually annotated by BRENDA team
Dengue virus-670994--Manually annotated by BRENDA team
Dengue virusi.e. DENV667226--Manually annotated by BRENDA team
Drosophila melanogaster-668745--Manually annotated by BRENDA team
Escherichia coli-668745--Manually annotated by BRENDA team
Geobacter sulfurreducens-668745--Manually annotated by BRENDA team
Hepatitis C virus-656494--Manually annotated by BRENDA team
Hepatitis C virusi.e. HCV667226--Manually annotated by BRENDA team
Homo sapiens-209890, 668745, 688347--Manually annotated by BRENDA team
Human coronavirus 229EHCoV-229E670074--Manually annotated by BRENDA team
Human enterovirus C-209889--Manually annotated by BRENDA team
Japanese encephalitis virusi.e. JEV667226--Manually annotated by BRENDA team
Klebsiella pneumoniae-668745--Manually annotated by BRENDA team
Leuconostoc mesenteroides-668745--Manually annotated by BRENDA team
Magnetococcus sp.strain mc-1668745--Manually annotated by BRENDA team
Magnetococcus sp. mc-1strain mc-1668745--Manually annotated by BRENDA team
mammalian orthoreovirusmORV669274--Manually annotated by BRENDA team
mammalian orthoreovirus mORVmORV669274--Manually annotated by BRENDA team
Mus musculusfemale C57BL/6J mice667903--Manually annotated by BRENDA team
Neospora caninum-718494C9K7J7UniProtManually annotated by BRENDA team
no activity in tobacco mosaic virus-668616--Manually annotated by BRENDA team
Nostoc punctiforme-668745--Manually annotated by BRENDA team
Oryctolagus cuniculus-209874--Manually annotated by BRENDA team
Pisum sativumpea209882, 209883, 209884, 209885--Manually annotated by BRENDA team
potato virus APVA, a poteyvirus668616--Manually annotated by BRENDA team
potato virus XPVX, a potexvirus668616--Manually annotated by BRENDA team
Pseudomonas aeruginosastrain PAK, genes pilB, pilT and pilU688871--Manually annotated by BRENDA team
Pseudomonas putida-668745--Manually annotated by BRENDA team
Rattus norvegicus-209876, 209878, 209881, 209886, 668745--Manually annotated by BRENDA team
Rattus norvegicusmale sprague-dawley rats671005--Manually annotated by BRENDA team
Saccharomyces cerevisiae-667694--Manually annotated by BRENDA team
Saccharophagus degradans-668745--Manually annotated by BRENDA team
Southampton virus-656663Q04544SwissProtManually annotated by BRENDA team
Staphylococcus epidermidis-668745--Manually annotated by BRENDA team
Sulfolobus solfataricus-690616, 694432--Manually annotated by BRENDA team
Sulfolobus solfataricusgene sso0909690865Q97ZJ7UniProtManually annotated by BRENDA team
Sulfolobus tokodaii-695127--Manually annotated by BRENDA team
Synechocystis sp.-668745--Manually annotated by BRENDA team
Thermotoga maritima-668745--Manually annotated by BRENDA team
Toxoplasma gondii-718494Q27893UniProtManually annotated by BRENDA team
Vaccinia virus-209880--Manually annotated by BRENDA team
Vibrio parahaemolyticus-668745--Manually annotated by BRENDA team
Vibrio vulnificus-668745--Manually annotated by BRENDA team
West Nile virus-656466, 656664--Manually annotated by BRENDA team
West Nile virusi.e. WNV667226--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
2',3'-didehydro-thymidine triphosphate + H2O2',3'-didehydro-thymidine diphosphate + phosphate
show the reaction diagram		Hepatitis C virus--656494--?
3'-azido-2',3'-dideoxythymidine triphosphate + H2O3'-azido-2',3'-dideoxythymidine diphosphate + phosphate
show the reaction diagram		Hepatitis C virus--656494--?
ADP + H2OAMP + phosphate
show the reaction diagram		Homo sapiens--688347--?
ADP + H2OAMP + phosphate
show the reaction diagram		Bos taurus-0.5 mM, 25% relative activity towards ADP compared to CTP, no activity towards AMP696153--?
ADP + H2OAMP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6relative substrate activity toward a variety of nucleoside di- and triphosphate substrates696275--?
ATP + H2OADP + phosphate
show the reaction diagram		Homo sapiens--688347--?
ATP + H2OADP + phosphate
show the reaction diagram		Rattus norvegicus--209878---
ATP + H2OADP + phosphate
show the reaction diagram		Rattus norvegicus--671005--?
ATP + H2OADP + phosphate
show the reaction diagram		Saccharomyces cerevisiae--667694--?
ATP + H2OADP + phosphate
show the reaction diagram		Sulfolobus solfataricus--690616, 694432--?
ATP + H2OADP + phosphate
show the reaction diagram		Hepatitis C virus--656494--?
ATP + H2OADP + phosphate
show the reaction diagram		potato virus A--668616--?
ATP + H2OADP + phosphate
show the reaction diagram		Human enterovirus C--209889---
ATP + H2OADP + phosphate
show the reaction diagram		Sulfolobus tokodaii--695127--?
ATP + H2OADP + phosphate
show the reaction diagram		West Nile virus--656466, 656664--?
ATP + H2OADP + phosphate
show the reaction diagram		Classical swine fever virus--684774--?
ATP + H2OADP + phosphate
show the reaction diagram		Southampton virusQ04544-656663--?
ATP + H2OADP + phosphate
show the reaction diagram		Human coronavirus 229E--670074--?
ATP + H2OADP + phosphate
show the reaction diagram		potato virus X--668616--?
ATP + H2OADP + phosphate
show the reaction diagram		mammalian orthoreovirus--669274--?
ATP + H2OADP + phosphate
show the reaction diagram		Toxoplasma gondiiQ27893-718494--?
ATP + H2OADP + phosphate
show the reaction diagram		Neospora caninumC9K7J7-718494--?
ATP + H2OADP + phosphate
show the reaction diagram		Dengue virus-ATPase activity670994--?
ATP + H2OADP + phosphate
show the reaction diagram		Dengue virus, Hepatitis C virus, West Nile virus, Japanese encephalitis virus-NTPase activity667226--?
ATP + H2OADP + phosphate
show the reaction diagram		Homo sapiens-HCR-NTPase contains both residues E113 and G115, and has nearly identical hydrolysis rates for ATP and GTP688347--?
ATP + H2OADP + phosphate
show the reaction diagram		Pseudomonas aeruginosa-recombinant His-tagged PilB, PilT, and PilU show ATPase activity in vitro with requirement for three invariant acidic residues in the Asp Box motif, and for two invariant His residues in the His Box motif to varying extents, overview688871--?
ATP + H2OADP + phosphate
show the reaction diagram		Sulfolobus tokodaii-Rad55B has a very weak ATPase activity and the activity is not stimulated by DNA695127--?
ATP + H2OADP + phosphate
show the reaction diagram		Sulfolobus solfataricus-the affinity of Rad54 towards DNA increases in the presence of ATP or ADP694432--?
ATP + H2OADP + phosphate
show the reaction diagram		Bos taurus-0.5 mM, 32% relative activity compared to CTP696153--?
ATP + H2OADP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6relative substrate activity toward a variety of nucleoside di- and triphosphate substrates696275--?
ATP + H2OADP + phosphate
show the reaction diagram		Japanese encephalitis virus JEV, Japanese encephalitis virus JaOH0566-NTPase activity667226--?
ATP + H2OADP + phosphate
show the reaction diagram		Human enterovirus C Sabin--209889---
ATP + H2OADP + phosphate
show the reaction diagram		mammalian orthoreovirus mORV--669274--?
ATP + H2OADP + phosphate
show the reaction diagram		Human enterovirus C Mahoney--209889---
CDP + H2OCMP + phosphate
show the reaction diagram		Bos taurus-0.5 mM, 41% relative activity towards CDP, no activity towards CMP696153--?
CDP + H2OCMP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6first nudix hydrolase observed to have a marked preference for CDP and CTP, activity at the site of the phosphodiester bond corroborated using 31P NMR spectroscopy, nucleophilic substitution at the beta-phosphorus696275--?
CDP + H2OCMP + phosphate
show the reaction diagram		Bos taurus-0.5 mM, 41% relative activity towards CDP compared to CTP, no activity towards CMP696153--?
CTP + H2OCDP + phosphate
show the reaction diagram		Homo sapiens--688347--?
CTP + H2OCDP + phosphate
show the reaction diagram		Saccharomyces cerevisiae--667694--?
CTP + H2OCDP + phosphate
show the reaction diagram		Classical swine fever virus--684774--?
CTP + H2OCDP + phosphate
show the reaction diagram		mammalian orthoreovirus--669274--?
CTP + H2OCDP + phosphate
show the reaction diagram		Southampton virusQ04544about 35% of activity with ATP656663--?
CTP + H2OCDP + phosphate
show the reaction diagram		Bos taurus-0.5 mM, 100% relative activity696153--?
CTP + H2OCDP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6first nudix hydrolase observed to have a marked preference for CDP and CTP, activity at the site of the phosphodiester bond corroborated using 31P NMR spectroscopy, nucleophilic substitution at the beta-phosphorus696275--?
CTP + H2OCDP + phosphate
show the reaction diagram		mammalian orthoreovirus mORV--669274--?
dADP + H2OdAMP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6relative substrate activity toward a variety of nucleoside di- and triphosphate substrates696275--?
dATP + H2OdADP + phosphate
show the reaction diagram		Saccharomyces cerevisiae--667694--?
dATP + H2OdADP + phosphate
show the reaction diagram		Hepatitis C virus--656494--?
dATP + H2OdADP + phosphate
show the reaction diagram		Classical swine fever virus--684774--?
dATP + H2OdADP + phosphate
show the reaction diagram		Human coronavirus 229E--670074--?
dATP + H2OdADP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6relative substrate activity toward a variety of nucleoside di- and triphosphate substrates696275--?
dCDP + H2OdCMP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6next most preferred substrates with a relative activity of <50% compared to CDP, activity at the site of the phosphodiester bond corroborated using 31P NMR spectroscopy696275--?
dCTP + H2OdCDP + phosphate
show the reaction diagram		Saccharomyces cerevisiae--667694--?
dCTP + H2OdCDP + phosphate
show the reaction diagram		Hepatitis C virus--656494--?
dCTP + H2OdCDP + phosphate
show the reaction diagram		Classical swine fever virus--684774--?
dCTP + H2OdCDP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6next most preferred substrates with a relative activity of <50% compared to CDP, activity at the site of the phosphodiester bond corroborated using 31P NMR spectroscopy696275--?
ddCTP + H2OddCDP + phosphate
show the reaction diagram		Hepatitis C virus--656494--?
ddTTP + H2OddTDP + phosphate
show the reaction diagram		Hepatitis C virus--656494--?
dGDP + H2OdGMP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6relative substrate activity toward a variety of nucleoside di- and triphosphate substrates696275--?
dGTP + H2OdGDP + phosphate
show the reaction diagram		Saccharomyces cerevisiae--667694--?
dGTP + H2OdGDP + phosphate
show the reaction diagram		Classical swine fever virus--684774--?
dGTP + H2OdGDP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6relative substrate activity toward a variety of nucleoside di- and triphosphate substrates696275--?
dITP + H2OdIDP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6-696275--?
dNTP + H2OdNDP + phosphate
show the reaction diagram		Bacillus subtilis--669092--?
dTTP + H2OdTDP + phosphate
show the reaction diagram		Saccharomyces cerevisiae--667694--?
dTTP + H2OTDP + phosphate
show the reaction diagram		Hepatitis C virus--656494--?
dUTP + H2OdUDP + phosphate
show the reaction diagram		Classical swine fever virus--684774--?
GDP + H2OGMP + phosphate
show the reaction diagram		Bos taurus-0.5 mM, 19% relative activity towards GDP compared to CTP, no activity towards GMP696153--?
GDP + H2OGMP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6relatively low substrate activity696275--?
GTP + H2OGDP + phosphate
show the reaction diagram		Homo sapiens--688347--?
GTP + H2OGDP + phosphate
show the reaction diagram		Saccharomyces cerevisiae--667694--?
GTP + H2OGDP + phosphate
show the reaction diagram		Classical swine fever virus--684774--?
GTP + H2OGDP + phosphate
show the reaction diagram		Human coronavirus 229E--670074--?
GTP + H2OGDP + phosphate
show the reaction diagram		mammalian orthoreovirus--669274--?
GTP + H2OGDP + phosphate
show the reaction diagram		Rattus norvegicus-preferred substrate671005--?
GTP + H2OGDP + phosphate
show the reaction diagram		Southampton virusQ04544about 90% of activity with ATP656663--?
GTP + H2OGDP + phosphate
show the reaction diagram		Homo sapiens-HCR-NTPase contains both residues E113 and G115, and has nearly identical hydrolysis rates for ATP and GTP688347--?
GTP + H2OGDP + phosphate
show the reaction diagram		Bos taurus-0.5 mM, 30% relative activity compared to CTP696153--?
GTP + H2OGDP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6relatively low substrate activity696275--?
GTP + H2OGDP + phosphate
show the reaction diagram		mammalian orthoreovirus mORV--669274--?
IDP + H2OIMP + phosphate
show the reaction diagram		Bos taurus-0.5 mM, 41% relative activity towards IDP compared to CTP, 5.3% relative activity towards IMP compared to CTP696153--?
IDP + H2OIMP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6activity at the site of the phosphodiester bond corroborated using 31P NMR spectroscopy, low substrate activity696275--?
IDP + H2OIMP + phosphate
show the reaction diagram		Bos taurus-0.5 mM, 41% relative activity towards IDP, 5.3% relative activity towards IMP compared to CTP696153--?
ITP + H2OIDP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6-696275--?
ITP + H2OIDP + phosphate
show the reaction diagram		Bos taurus-0.5 mM, 58% relative activity compared to CTP696153--?
L-dATP + H2OL-dADP + phosphate
show the reaction diagram		Hepatitis C virus--656494--?
L-dCTP + H2OCDP + phosphate
show the reaction diagram		Hepatitis C virus--656494--?
NDP + H2ONMP + phosphate
show the reaction diagram		Rattus norvegicus--209881--?
NTP + H2ONDP + phosphate
show the reaction diagram		Drosophila melanogaster, Escherichia coli, Homo sapiens--668745--?
NTP + H2ONDP + phosphate
show the reaction diagram		Rattus norvegicus--209881, 209886, 668745--?
NTP + H2ONDP + phosphate
show the reaction diagram		Oryctolagus cuniculus--209874--?
NTP + H2ONDP + phosphate
show the reaction diagram		Pseudomonas aeruginosa--688871--?
NTP + H2ONDP + phosphate
show the reaction diagram		Pseudomonas putida, Caenorhabditis elegans, Leuconostoc mesenteroides, Klebsiella pneumoniae, Corynebacterium glutamicum, Staphylococcus epidermidis--668745--?
NTP + H2ONDP + phosphate
show the reaction diagram		Vaccinia virus--209880--?
NTP + H2ONDP + phosphate
show the reaction diagram		Agrobacterium tumefaciens, Clostridium perfringens, Vibrio parahaemolyticus, Anabaena sp., Synechocystis sp., Thermotoga maritima, Deinococcus radiodurans--668745--?
NTP + H2ONDP + phosphate
show the reaction diagram		Human enterovirus C--209889--?
NTP + H2ONDP + phosphate
show the reaction diagram		Nostoc punctiforme, Cupriavidus metallidurans, Saccharophagus degradans, Geobacter sulfurreducens, Vibrio vulnificus, Corynebacterium efficiens, Magnetococcus sp.--668745--?
NTP + H2ONDP + phosphate
show the reaction diagram		Dengue virus-NTPase activity670994--?
NTP + H2ONDP + phosphate
show the reaction diagram		Geobacter sulfurreducens DL1, Saccharophagus degradans 2-40T, Magnetococcus sp. mc-1--668745--?
NTP + H2ONDP + phosphate
show the reaction diagram		Human enterovirus C Sabin--209889--?
NTP + H2ONDP + phosphate
show the reaction diagram		Geobacter sulfurreducens DL-1--668745--?
NTP + H2ONDP + phosphate
show the reaction diagram		Human enterovirus C Mahoney--209889--?
RNA + H2O?
show the reaction diagram		mammalian orthoreovirus-removal of the gamma-phosphate669274--?
ssDNA + H2O?
show the reaction diagram		Saccharomyces cerevisiae-removal of gamma-phosphate from nucleotides667694--?
ssRNA + H2O?
show the reaction diagram		Saccharomyces cerevisiae-removal of gamma-phosphate from nucleotides667694--?
TDP + H2OTMP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6relative substrate activity toward a variety of nucleoside di- and triphosphate substrates696275--?
TTP + H2OTDP + phosphate
show the reaction diagram		Homo sapiens--209890---
TTP + H2OTDP + phosphate
show the reaction diagram		Homo sapiens--688347--?
TTP + H2OTDP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6relative substrate activity toward a variety of nucleoside di- and triphosphate substrates696275--?
UDP + H2OUMP + phosphate
show the reaction diagram		Bos taurus-0.5 mM, 32% relative activity compared to CTP, 8.2% relative activity towards UMP compared to CTP696153--?
UDP + H2OUMP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6relative low substrate activity696275--?
UDP + H2OUMP + phosphate
show the reaction diagram		Bos taurus-0.5 mM, 32% relative activity, 8.2% relative activity towards UMP compared to CTP696153--?
UTP + H2OUDP + phosphate
show the reaction diagram		Homo sapiens--688347--?
UTP + H2OUDP + phosphate
show the reaction diagram		Saccharomyces cerevisiae--667694--?
UTP + H2OUDP + phosphate
show the reaction diagram		Classical swine fever virus--684774--?
UTP + H2OUDP + phosphate
show the reaction diagram		mammalian orthoreovirus--669274--?
UTP + H2OUDP + phosphate
show the reaction diagram		potato virus A, potato virus X-recombinant enzyme668616--?
UTP + H2OUDP + phosphate
show the reaction diagram		Southampton virusQ04544about 35% of activity with ATP656663--?
UTP + H2OUDP + phosphate
show the reaction diagram		Bos taurus-0.5 mM, 66% relative activity compared to CTP696153--?
UTP + H2OUDP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6relative low substrate activity696275--?
UTP + H2OUDP + phosphate
show the reaction diagram		mammalian orthoreovirus mORV--669274--?
XTP + H2OXDP + phosphate
show the reaction diagram		Bos taurus-0.5 mM, 40% relative activity compared to CTP696153--?
L-dTTP + H2OL-dTDP + phosphate
show the reaction diagram		Hepatitis C virus--656494--?
additional information?-Hepatitis C virus-multifunctional protein, possessing protease, NTPase and helicase activities. These activities are essential for the virus life cycle656494---
additional information?-West Nile virus-helicase activity with RNA duplex656466---
additional information?-West Nile virus-helicase activity, the enzyme catalyzes the unwinding of DNA duplex656664---
additional information?-Hepatitis C virus-multifunctional protein, possessing protease, NTPase and helicase activities. Different stereoselectivity in nucleoside triphosphate utilisation suggests that NTPase and helicase activities are coupled by a nucleotide-dependent rate limiting step656494---
additional information?-Southampton virusQ04544the enzyme does not possess helicase activity656663---
additional information?-potato virus A, potato virus X-the coat protein of the plant virus exhibits NTPase activity, the coat proteins are involved in different stages of the viral life cycle such as virion assembyl, replication, movement, vector transmission, and regulation of host defense response668616---
additional information?-Saccharomyces cerevisiae-the DEAH-box splicing factor Prp22 is important in the second transesterification step of pre-mRNA splicing and is essential for release of mature mRNA from the splicosome667694---
additional information?-Dengue virus-the enzyme is involved in RNA replication and capping, overview670994---
additional information?-Saccharomyces cerevisiae-DEAH-box splicing factor Prp22 shows NTPase, RNA-binding, and RNA helicase activities, ATP binding leads to dissociation of the protein from RNA, RNA binding activates the NTPase activity667694---
additional information?-Dengue virus, Hepatitis C virus, West Nile virus, Japanese encephalitis virus-multifunctional enzyme showing nucleoside triphosphatase NTPase/RNA helicase and a 5'-RNA triphosphatase RTPase activities667226---
additional information?-Human coronavirus 229E-the enzyme hydrolyzes all natural ribonucleotides and nucleotides, the enzyme also mediates RNA 5'-triphosphatase activity using the NTPase active site, this activity is inhibited by ATP, overview670074---
additional information?-potato virus X-the enzyme hydrolyzes ribonucleotides and deoxyribonucleotides668616---
additional information?-Dengue virus-the multifunctional enzyme shows RNA helicase, nucleotide 5'-triphosphatase, and RNA 5'-triphosphatase activities, which are located on the C-terminal 54-kDa domain, overview670994---
additional information?-mammalian orthoreovirus-the virus core particles, an icosahedral multienzyme complex, and the transcriptase cofactor my2 show nucleoside triphosphatase activity669274---
additional information?-Classical swine fever virus-the viral NS3 protein, i.e. NS3FL, and its N-terminal truncated version ntNS3, recombinantly expressed in Escherichia coli, contain specific polynucleotide-stimulated NTPase acitivity, the enzyme activity is essential for viral replication684774---
additional information?-Homo sapiens-HCR-NTPase is a non-selective NTPase with only slightly higher activity for purine nucleoside triphosphates, and with a comparatively low rate of hydrolysis, structural basis of HCR-NTPase activity, overview688347---
additional information?-Classical swine fever virus-the conserved motif I, GXGK232T, is required for NTPase activity of the viral NS3 protein, while motif III is not684774---
additional information?-Sulfolobus solfataricusQ97ZJ7Sso0909 is an orthologue of the eukaryotic endosomal sorting complex required for transport, ESCRT, ATPase Vps4, i.e. vacular protein sorting 4. Sso0909 is not involved in a cellular protection mechanism, but is required during normal cell growth690865---
additional information?-Sulfolobus solfataricus-the N-terminal AAA+ ATPase domains of Orc1/Cdc6 proteins show DNA-binding activities and functional interactions with other Cdc6 proteins, on duplex DNA substrates derived from the origins of Sulfolobus solfataricus, overview. ATPase domain of Cdc6-2 retains both DNA-binding activity and regulating effect on that of Cdc6-3 at the origin, overview690616---
additional information?-Sulfolobus solfataricus-a DNA-dependent ATPase, mechano-chemical cycle of the catalytic domain of the Rad54 homologue Rad54cd, fluorescence resonance energy transfer analysis. The open conformation is converted into a closed conformation after DNA binding, a re-arrangement of domains occurs, which is due to phosphate release and not due to ATP binding, overview694432---
additional information?-Sulfolobus solfataricus-Cdc6-1 significantly improves DNA-binding activity at the forked substrate, but only shows a very weak ability towards the blunt DNA substrate690616---
additional information?-Sulfolobus tokodaii-Rad55B also performs DNA repair, DNA binding using single-stranded or double-stranded 84mer oligonucleotide as substrate, Rad55B is a homologue of RadA, that is able to perform DNA strand exchanges using circular single-stranded M13mp18 virion DNA as substrate, overview695127---
additional information?-Japanese encephalitis virus JEV, Japanese encephalitis virus JaOH0566-multifunctional enzyme showing nucleoside triphosphatase NTPase/RNA helicase and a 5'-RNA triphosphatase RTPase activities667226---
additional information?-Classical swine fever virus CSFV-the viral NS3 protein, i.e. NS3FL, and its N-terminal truncated version ntNS3, recombinantly expressed in Escherichia coli, contain specific polynucleotide-stimulated NTPase acitivity, the enzyme activity is essential for viral replication, the conserved motif I, GXGK232T, is required for NTPase activity of the viral NS3 protein, while motif III is not684774---
additional information?-mammalian orthoreovirus mORV-the virus core particles, an icosahedral multienzyme complex, and the transcriptase cofactor my2 show nucleoside triphosphatase activity669274---

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
ADP + H2OAMP + phosphate
show the reaction diagram		Homo sapiens--688347--
ADP + H2OAMP + phosphate
show the reaction diagram		Bos taurus-0.5 mM, 25% relative activity towards ADP compared to CTP, no activity towards AMP696153--
ADP + H2OAMP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6relative substrate activity toward a variety of nucleoside di- and triphosphate substrates696275--
ATP + H2OADP + phosphate
show the reaction diagram		Homo sapiens--688347--
ATP + H2OADP + phosphate
show the reaction diagram		Sulfolobus solfataricus--690616, 694432--
ATP + H2OADP + phosphate
show the reaction diagram		potato virus A--668616--
ATP + H2OADP + phosphate
show the reaction diagram		Sulfolobus tokodaii--695127--
ATP + H2OADP + phosphate
show the reaction diagram		Classical swine fever virus--684774--
ATP + H2OADP + phosphate
show the reaction diagram		potato virus X--668616--
ATP + H2OADP + phosphate
show the reaction diagram		Dengue virus-ATPase activity670994--
ATP + H2OADP + phosphate
show the reaction diagram		Dengue virus, Hepatitis C virus, West Nile virus, Japanese encephalitis virus-NTPase activity667226--
ATP + H2OADP + phosphate
show the reaction diagram		Bos taurus-0.5 mM, 32% relative activity compared to CTP696153--
ATP + H2OADP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6relative substrate activity toward a variety of nucleoside di- and triphosphate substrates696275--
ATP + H2OADP + phosphate
show the reaction diagram		Japanese encephalitis virus JEV, Japanese encephalitis virus JaOH0566-NTPase activity667226--
CDP + H2OCMP + phosphate
show the reaction diagram		Bos taurus-0.5 mM, 41% relative activity towards CDP, no activity towards CMP696153--
CDP + H2OCMP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6first nudix hydrolase observed to have a marked preference for CDP and CTP, activity at the site of the phosphodiester bond corroborated using 31P NMR spectroscopy, nucleophilic substitution at the beta-phosphorus696275--
CTP + H2OCDP + phosphate
show the reaction diagram		Homo sapiens--688347--
CTP + H2OCDP + phosphate
show the reaction diagram		Classical swine fever virus--684774--
CTP + H2OCDP + phosphate
show the reaction diagram		Bos taurus-0.5 mM, 100% relative activity696153--
CTP + H2OCDP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6first nudix hydrolase observed to have a marked preference for CDP and CTP, activity at the site of the phosphodiester bond corroborated using 31P NMR spectroscopy, nucleophilic substitution at the beta-phosphorus696275--
dADP + H2OdAMP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6relative substrate activity toward a variety of nucleoside di- and triphosphate substrates696275--
dATP + H2OdADP + phosphate
show the reaction diagram		Classical swine fever virus--684774--
dATP + H2OdADP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6relative substrate activity toward a variety of nucleoside di- and triphosphate substrates696275--
dCDP + H2OdCMP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6next most preferred substrates with a relative activity of <50% compared to CDP, activity at the site of the phosphodiester bond corroborated using 31P NMR spectroscopy696275--
dCTP + H2OdCDP + phosphate
show the reaction diagram		Classical swine fever virus--684774--
dCTP + H2OdCDP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6next most preferred substrates with a relative activity of <50% compared to CDP, activity at the site of the phosphodiester bond corroborated using 31P NMR spectroscopy696275--
dGDP + H2OdGMP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6relative substrate activity toward a variety of nucleoside di- and triphosphate substrates696275--
dGTP + H2OdGDP + phosphate
show the reaction diagram		Classical swine fever virus--684774--
dGTP + H2OdGDP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6relative substrate activity toward a variety of nucleoside di- and triphosphate substrates696275--
dITP + H2OdIDP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6-696275--
dUTP + H2OdUDP + phosphate
show the reaction diagram		Classical swine fever virus--684774--
GDP + H2OGMP + phosphate
show the reaction diagram		Bos taurus-0.5 mM, 19% relative activity towards GDP compared to CTP, no activity towards GMP696153--
GDP + H2OGMP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6relatively low substrate activity696275--
GTP + H2OGDP + phosphate
show the reaction diagram		Homo sapiens--688347--
GTP + H2OGDP + phosphate
show the reaction diagram		Classical swine fever virus--684774--
GTP + H2OGDP + phosphate
show the reaction diagram		Bos taurus-0.5 mM, 30% relative activity compared to CTP696153--
GTP + H2OGDP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6relatively low substrate activity696275--
IDP + H2OIMP + phosphate
show the reaction diagram		Bos taurus-0.5 mM, 41% relative activity towards IDP compared to CTP, 5.3% relative activity towards IMP compared to CTP696153--
IDP + H2OIMP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6activity at the site of the phosphodiester bond corroborated using 31P NMR spectroscopy, low substrate activity696275--
ITP + H2OIDP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6-696275--
NTP + H2ONDP + phosphate
show the reaction diagram		Drosophila melanogaster, Escherichia coli, Homo sapiens--668745--
NTP + H2ONDP + phosphate
show the reaction diagram		Rattus norvegicus--209881, 209886, 668745--
NTP + H2ONDP + phosphate
show the reaction diagram		Oryctolagus cuniculus--209874--
NTP + H2ONDP + phosphate
show the reaction diagram		Pseudomonas putida, Caenorhabditis elegans, Leuconostoc mesenteroides, Klebsiella pneumoniae, Corynebacterium glutamicum, Staphylococcus epidermidis--668745--
NTP + H2ONDP + phosphate
show the reaction diagram		Vaccinia virus--209880--
NTP + H2ONDP + phosphate
show the reaction diagram		Agrobacterium tumefaciens, Clostridium perfringens, Vibrio parahaemolyticus, Anabaena sp., Synechocystis sp., Thermotoga maritima, Deinococcus radiodurans--668745--
NTP + H2ONDP + phosphate
show the reaction diagram		Human enterovirus C--209889--
NTP + H2ONDP + phosphate
show the reaction diagram		Nostoc punctiforme, Cupriavidus metallidurans, Saccharophagus degradans, Geobacter sulfurreducens, Vibrio vulnificus, Corynebacterium efficiens, Magnetococcus sp.--668745--
NTP + H2ONDP + phosphate
show the reaction diagram		Dengue virus-NTPase activity670994--
NTP + H2ONDP + phosphate
show the reaction diagram		Geobacter sulfurreducens DL1, Saccharophagus degradans 2-40T, Magnetococcus sp. mc-1--668745--
NTP + H2ONDP + phosphate
show the reaction diagram		Human enterovirus C Sabin--209889--
NTP + H2ONDP + phosphate
show the reaction diagram		Geobacter sulfurreducens DL-1--668745--
NTP + H2ONDP + phosphate
show the reaction diagram		Human enterovirus C Mahoney--209889--
RNA + H2O?
show the reaction diagram		mammalian orthoreovirus-removal of the gamma-phosphate669274--
TDP + H2OTMP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6relative substrate activity toward a variety of nucleoside di- and triphosphate substrates696275--
TTP + H2OTDP + phosphate
show the reaction diagram		Homo sapiens--688347--
TTP + H2OTDP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6relative substrate activity toward a variety of nucleoside di- and triphosphate substrates696275--
UDP + H2OUMP + phosphate
show the reaction diagram		Bos taurus-0.5 mM, 32% relative activity compared to CTP, 8.2% relative activity towards UMP compared to CTP696153--
UDP + H2OUMP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6relative low substrate activity696275--
UTP + H2OUDP + phosphate
show the reaction diagram		Homo sapiens--688347--
UTP + H2OUDP + phosphate
show the reaction diagram		Classical swine fever virus--684774--
UTP + H2OUDP + phosphate
show the reaction diagram		Bos taurus-0.5 mM, 66% relative activity compared to CTP696153--
UTP + H2OUDP + phosphate
show the reaction diagram		Deinococcus radioduransQ9RXX6relative low substrate activity696275--
XTP + H2OXDP + phosphate
show the reaction diagram		Bos taurus-0.5 mM, 40% relative activity compared to CTP696153--
ITP + H2OIDP + phosphate
show the reaction diagram		Bos taurus-0.5 mM, 58% relative activity compared to CTP696153--
additional information?-Hepatitis C virus-multifunctional protein, possessing protease, NTPase and helicase activities. These activities are essential for the virus life cycle656494--
additional information?-potato virus A, potato virus X-the coat protein of the plant virus exhibits NTPase activity, the coat proteins are involved in different stages of the viral life cycle such as virion assembyl, replication, movement, vector transmission, and regulation of host defense response668616--
additional information?-Saccharomyces cerevisiae-the DEAH-box splicing factor Prp22 is important in the second transesterification step of pre-mRNA splicing and is essential for release of mature mRNA from the splicosome667694--
additional information?-Dengue virus-the enzyme is involved in RNA replication and capping, overview670994--
additional information?-Classical swine fever virus-the viral NS3 protein, i.e. NS3FL, and its N-terminal truncated version ntNS3, recombinantly expressed in Escherichia coli, contain specific polynucleotide-stimulated NTPase acitivity, the enzyme activity is essential for viral replication684774--
additional information?-Sulfolobus solfataricusQ97ZJ7Sso0909 is an orthologue of the eukaryotic endosomal sorting complex required for transport, ESCRT, ATPase Vps4, i.e. vacular protein sorting 4. Sso0909 is not involved in a cellular protection mechanism, but is required during normal cell growth690865--
additional information?-Sulfolobus solfataricus-the N-terminal AAA+ ATPase domains of Orc1/Cdc6 proteins show DNA-binding activities and functional interactions with other Cdc6 proteins, on duplex DNA substrates derived from the origins of Sulfolobus solfataricus, overview. ATPase domain of Cdc6-2 retains both DNA-binding activity and regulating effect on that of Cdc6-3 at the origin, overview690616--
additional information?-Classical swine fever virus CSFV-the viral NS3 protein, i.e. NS3FL, and its N-terminal truncated version ntNS3, recombinantly expressed in Escherichia coli, contain specific polynucleotide-stimulated NTPase acitivity, the enzyme activity is essential for viral replication684774--

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
No entries in this field

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
Ba2+Bos taurus-5 mM, unable to stimulate enzyme activity696153
Ca2+Oryctolagus cuniculus--209874
Ca2+Rattus norvegicus-activates at low concentrations209876
Ca2+Rattus norvegicus--209881, 209886
Ca2+potato virus A-activates slightly, much less efficient than Mg2+668616
Ca2+potato virus X-activates, less efficient than Mg2+668616
Ca2+mammalian orthoreovirus, mammalian orthoreovirus mORV-activates669274
Ca2+Bos taurus-5 mM, 52% relative enzyme activity in presence of696153
Co2+Bos taurus-5 mM, 60% relative enzyme activity in presence of696153
Co2+Deinococcus radioduransQ9RXX6lower activity in presence of Co2+ than in presence of Mn2+696275
Cs+Rattus norvegicus-at concentration below 20 mM209886
Cu2+potato virus A, potato virus X-activates slightly, much less efficient than Mg2+668616
Cu2+Bos taurus-5 mM, 46% relative enzyme activity in presence of696153
K+Rattus norvegicus-activates at concentration below 20 mM209886
K+West Nile virus, West Nile virus NY99, West Nile virus WNV-100-300 mM, activation of ATPase reaction to about 200% of control656664
Mg2+Oryctolagus cuniculus--209874
Mg2+Vaccinia virus--209880
Mg2+Rattus norvegicus--209876, 209881, 209886
Mg2+Southampton virusQ0454420 mM, about 50% of the maximal activation obtained with Mn2+656663
Mg2+West Nile virus, West Nile virus NY99, West Nile virus WNV-required. 150% of the control at an optimum Mg2+ concentration of 1-3 mM656664
Mg2+Saccharomyces cerevisiae-required667694
Mg2+potato virus A, potato virus X-best metal ion for enzyme activation668616
Mg2+Bacillus subtilis--669092
Mg2+mammalian orthoreovirus, mammalian orthoreovirus mORV-activates669274
Mg2+Dengue virus, Dengue virus DENV, Dengue virus PL046-strictly dependent on, coordination of Mg2+ by the Walker B motif, required for all three activities, the RNA helicase, nucleotide 5'-triphosphatase, and RNA 5'-triphosphatase activity670994
Mg2+Classical swine fever virus, Classical swine fever virus CSFV-required, activates684774
Mg2+Homo sapiens--688347
Mg2+Pseudomonas aeruginosa--688871
Mg2+Sulfolobus solfataricus--694432
Mg2+Sulfolobus tokodaii--695127
Mg2+Bos taurus-5 mM, 100% relative enzyme activity in presence of696153
Mg2+Deinococcus radioduransQ9RXX6highest value for Mg2+ requirement in metal profile determined696275
Mn2+Vaccinia virus--209880
Mn2+Southampton virusQ04544activates, 3.5-5 mM MnCl2 are optimal at an ATP concentration of 0.5 mM656663
Mn2+potato virus A, potato virus X-activates, less efficient than Mg2+668616
Mn2+mammalian orthoreovirus, mammalian orthoreovirus mORV-activates669274
Mn2+Bos taurus-5 mM, 73% relative enzyme activity in presence of696153
Na+Rattus norvegicus-activates at concentration below 20 mM209886
Na+West Nile virus, West Nile virus NY99, West Nile virus WNV-100-300 mM, activation of ATPase reaction to about 200% of control656664
NaClDengue virus, Dengue virus DENV, Dengue virus PL046, Hepatitis C virus, Japanese encephalitis virus, Japanese encephalitis virus JaOH0566, Japanese encephalitis virus JEV, West Nile virus, West Nile virus NY99, West Nile virus WNV-stimulates667226
Rb+Rattus norvegicus-activates at concentration below 20 mM209886
Zn2+potato virus A, potato virus X-activates slightly, much less efficient than Mg2+668616
Zn2+Human coronavirus 229E-the enzyme contains an N-terminal zinc binding domain670074
Zn2+Bos taurus-5 mM, 34% relative enzyme activity in presence of696153
Mn2+Deinococcus radioduransQ9RXX6higher activity in presence of Mn2+ than of Co2+696275
additional informationpotato virus A, potato virus X-the enzyme is dependent on divalent cations668616
additional informationmammalian orthoreovirus, mammalian orthoreovirus mORV-the enzyme activity depends on divalent cations and prefers Mg2+, Ca2+, and Mn2+669274

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
1-beta-D-ribofuranosyl-1H-benzotriazoleDengue virus, Hepatitis C virus, Japanese encephalitis virus, West Nile virus--667226 2D-image
1-beta-D-ribofuranosyl-4,5,6,7-tetrabromo-1H-benzotriazoleDengue virus, Hepatitis C virus, Japanese encephalitis virus, West Nile virus--667226 2D-image
1-beta-D-ribofuranosyl-4,5,6,7-tetramethyl-1H-benzotriazoleDengue virus, Hepatitis C virus, Japanese encephalitis virus, West Nile virus--667226 2D-image
1-beta-D-ribofuranosyl-4,7-dibromo-1H-benzotriazoleDengue virus, Hepatitis C virus, Japanese encephalitis virus, West Nile virus--667226 2D-image
1-chloroethyl-4,5,6,7-tetrabromo-1H-benzotriazoleDengue virus, Hepatitis C virus, Japanese encephalitis virus, West Nile virus--667226 2D-image
1-ethyl-4,5,6,7-tetrabromo-1H-benzotriazoleDengue virus, Hepatitis C virus, Japanese encephalitis virus, Japanese encephalitis virus JaOH0566, Japanese encephalitis virus JEV, West Nile virus--667226 2D-image
1-hydroxyethyl-4,5,6,7-tetrabromo-1H-benzotriazoleDengue virus, Hepatitis C virus, Japanese encephalitis virus, West Nile virus--667226 2D-image
1-methyl-4,5,6,7-tetrabromo-1H-benzotriazoleDengue virus, Hepatitis C virus, Japanese encephalitis virus, Japanese encephalitis virus JaOH0566, Japanese encephalitis virus JEV, West Nile virus--667226 2D-image
1-propyl-4,5,6,7-tetrabromo-1H-benzotriazoleDengue virus, Hepatitis C virus, Japanese encephalitis virus, West Nile virus--667226 2D-image
2',3'-didehydro-thymidine triphosphateHepatitis C virus-competitive to ATP656494 2D-image
2',3'-dideoxy-5-fluorocytidine triphosphateHepatitis C virus-competitive to ATP656494 2D-image
2-chloroethyl-4,5,6,7-tetrabromo-1H-benzotriazoleDengue virus, Hepatitis C virus, Japanese encephalitis virus, West Nile virus--667226 2D-image
2-ethyl-4,5,6,7-tetrabromo-1H-benzotriazoleDengue virus, Hepatitis C virus, Japanese encephalitis virus, Japanese encephalitis virus JaOH0566, Japanese encephalitis virus JEV, West Nile virus--667226 2D-image
2-hydroxyethyl-4,5,6,7-tetrabromo-1H-benzotriazoleDengue virus, Hepatitis C virus, Japanese encephalitis virus, West Nile virus--667226 2D-image
2-methyl-4,5,6,7-tetrabromo-1H-benzotriazoleDengue virus, Hepatitis C virus, Japanese encephalitis virus, Japanese encephalitis virus JaOH0566, Japanese encephalitis virus JEV, West Nile virus--667226 2D-image
2-propyl-4,5,6,7-tetrabromo-1H-benzotriazoleDengue virus, Hepatitis C virus, Japanese encephalitis virus, West Nile virus--667226 2D-image
3'-azido-2',3'-dideoxythymidine triphosphateHepatitis C virus-competitive to ATP656494 2D-image
4,5,6,7-tetrabromo-1H-benzotriazoleDengue virus, Hepatitis C virus, Japanese encephalitis virus, Japanese encephalitis virus JaOH0566, Japanese encephalitis virus JEV, West Nile virus--667226 2D-image
4,5-dihydro-8H-6-(N-decyl)amino-1-(beta-D-ribofuranosyl)imidazo[4,5-e][1,3]diazepine-4,8-dioneWest Nile virus-IC50: 30-100 mg/l656466 2D-image
4,5-dihydro-8H-6-(N-dodecyl)amino-1-(beta-D-ribofuranosyl)imidazo[4,5-e][1,3]diazepine-4,8-dioneWest Nile virus-IC50: 1-3 mg/l656466 2D-image
4,5-dihydro-8H-6-(N-dodecylamino)-1-(2'-deoxy-alpha-D-erythropentofuranosyl)imidazo[4,5-e][1,3]diazepine-4,8-dioneWest Nile virus-IC50: 3-10 mg/l656466 2D-image
4,5-dihydro-8H-6-(N-dodecylamino)-1-(2'-deoxy-beta-D-erythropentofuranosyl)imidazo[4,5-e][1,3]diazepine-4,8-dioneWest Nile virus-IC50: 3-10 mg/l656466 2D-image
4,5-dihydro-8H-6-(N-hexadecyl)amino-1-(beta-D-ribofuranosyl)imidazo[4,5-e][1,3]diazepine-4,8-dioneWest Nile virus-IC50: 250 mg/l656466 2D-image
4,5-dihydro-8H-6-(N-octadecyl)amino-1-(beta-D-ribofuranosyl)imidazo[4,5-e][1,3]diazepine-4,8-dioneWest Nile virus-IC50: 5.0 mg/l656466 2D-image
4,5-dihydro-8H-6-(N-tetradecyl)amino-1-(beta-D-ribofuranosyl)imidazo[4,5-e][1,3]diazepine-4,8-dioneWest Nile virus-IC50: 3-10 mg/l656466 2D-image
5,6-dichloro-1-beta-D-ribofuranosyl-1H-benzotriazoleDengue virus, Hepatitis C virus, Japanese encephalitis virus, West Nile virus--667226 2D-image
5-fluoro-2-selenocytosineWest Nile virus-IC50: 0.075 mM for NTPase reaction, no influence to helicase activity up to a concentration of 0.5 mM656664 2D-image
ADPWest Nile virus--656664 2D-image
ATPHepatitis C virus--656494 2D-image
beta,gamma-methylene-ATPRattus norvegicus--209876 2D-image
Ca2+Oryctolagus cuniculus-at high concentrations209874 2D-image
CaCl2Southampton virusQ04544in presence of Mn2+, 10 mM MgCl2 inhibits 20%656663 2D-image
cholineRattus norvegicus-at concentration above 20 mM209886 2D-image
Cs+Rattus norvegicus-at concentration above 20 mM209886 2D-image
dATPHepatitis C virus-competitive to ATP656494 2D-image
dCTPHepatitis C virus-competitive to ATP656494 2D-image
ddCTPHepatitis C virus-competitive to ATP656494 2D-image
ddTTPHepatitis C virus-competitive to ATP656494 2D-image
dGTPHepatitis C virus-competitive to ATP656494 2D-image
dTTPHepatitis C virus-competitive to ATP656494 2D-image
EDTApotato virus A, potato virus X--668616 2D-image
EDTAClassical swine fever virus, Classical swine fever virus CSFV--684774 2D-image
Guanidine hydrochlorideHuman enterovirus C, Human enterovirus C Mahoney, Human enterovirus C Sabin--209889 2D-image
HOClRattus norvegicus-inhibits the muclear NTPase, taurine acts as antagonist and reduces the hypochlorous acid toxicity671005 2D-image
iodoacetamideRattus norvegicus--209876 2D-image
K+Rattus norvegicus-at concentration above 20 mM209886 2D-image
K+West Nile virus-above 500 mM656664 2D-image
L-dATPHepatitis C virus-competitive to ATP656494 2D-image
L-dCTPHepatitis C virus-competitive to ATP656494 2D-image
L-ddCTPHepatitis C virus-competitive to ATP656494 2D-image
L-dTTPHepatitis C virus-competitive to ATP656494 2D-image
Mg2+West Nile virus-above 10 mM656664 2D-image
N-ethylmaleimideRattus norvegicus--209876 2D-image
Na+Rattus norvegicus-at concentration above 20 mM209886 2D-image
Na+West Nile virus-above 500 mM656664 2D-image
NaClSouthampton virusQ04544100 mM, inhibits ATP hydrolysis by 30%656663 2D-image
NaFRattus norvegicus--209876 2D-image
O6-benzyl-N7-chloroethylguanineWest Nile virus-weak inhibition of NTPase activity, enhances helicase activity656664 2D-image
O6-benzylguanineWest Nile virus-weak inhibitor of ATPase and helicase activity656664 2D-image
oligo(dA)25West Nile virus-above 0.5 mM, inhibits unwinding reaction656664-
oligomycinRattus norvegicus--209876 2D-image
p-hydroxymercuribenzoateRattus norvegicus--209876 2D-image
Phenylarsine oxideRattus norvegicus--209876 2D-image
polyASouthampton virusQ04544inhibits to a lesser extent than polyC and polyU656663 2D-image
polyCSouthampton virusQ045441 mg/l, 70% inhibition656663 2D-image
polyGSouthampton virusQ04544inhibits to a lesser extent than polyC and polyU656663 2D-image
polyUSouthampton virusQ045441 mg/l, 70% inhibition656663 2D-image
ProflavineRattus norvegicus--209876 2D-image
PropionateBos taurus-39% relative activity at a concentration of 0.5 mM, 63% relative activity at a concentration of 0.05 mM696153 2D-image
QuercitinRattus norvegicus--209876 2D-image
Rb+Rattus norvegicus-at concentration above 20 mM209886 2D-image
ribavirin-TPWest Nile virus-at ATP concentration equal to Km, IC50 of NTPase reaction is 0.4 mM, classical competitive inhibitor with regard to ATP. At ATP and DNA duplex concentrations corresponding to their KM-values an IC50 of 0.12 mM is measured. Inhibition reaches a maximum of 30% of the control at 0.45 mM and is not competitive with regard to ATP656664 2D-image
RNASouthampton virusQ04544homopolymeric RNA inhibits under otherwise optimal conditions656663 2D-image
rNTPpotato virus A, potato virus X-ribotrinucleotides inhibit the activity with gammaATP668616 2D-image
Sodium fluorideRattus norvegicus--209881 2D-image
MgCl2Southampton virusQ04544in presence of Mn2+, 10 mM MgCl2 inhibits 10%656663 2D-image
additional informationDengue virus, Hepatitis C virus, Japanese encephalitis virus, West Nile virus-inhibitor cytotoxicity study, overview667226-
additional informationSulfolobus solfataricus-the ATPase domain of Cdc6-3, although having lost much of its DNA-binding activity from the origin, inhibits both Cdc6-1 and Cdc6-2690616-

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
2-oxoglutarateBos taurus-stimulating in physiological concentrations, 148% relative activity at a concentration of 0.5 mM, 128% relative activity at a concentration of 0.05 mM696153 2D-image
4,5-dihydro-8H-6-(N-phenyl)amino-1-(beta-D-ribofuranosyl)imidazo[4,5-e][1,3]diazepine-4,8-dioneWest Nile virus, West Nile virus NY99, West Nile virus WNV-activates656466 2D-image
acetyl-CoABos taurus-stimulating in physiological concentrations, 148% relative activity at a concentration of 0.5 mM, 127% relative activity at a concentration of 0.05 mM696153 2D-image
cholineRattus norvegicus-activates at concentration below 20 mM209886 2D-image
D-fructose 1,6-bisphosphateBos taurus-stimulating in physiological concentrations, 155% relative activity at a concentration of 0.5 mM, 126% relative activity at a concentration of 0.05 mM696153 2D-image
O6-benzyl-N7-chloroethylguanineWest Nile virus, West Nile virus NY99, West Nile virus WNV-weak inhibition of NTPase activity, enhances helicase activity up to 850% of control656664 2D-image
O6-benzyl-N9-chloroethylguanineWest Nile virus, West Nile virus NY99, West Nile virus WNV-stimulates NTPase activity with a maximum effect of 350% of control at 0.65 mM, enhances helicase activity up to 220% of control656664 2D-image
poly dA-poly dT-DNASulfolobus solfataricus-a nucleic acid-stimulated DNA-dependent ATPase694432-
Poly(A)Saccharomyces cerevisiae-stimulates the ATP hydrolysis dependent on the chain length, highest activation by poly(A)40 comapred to shorter molecules667694 2D-image
Poly(dA)West Nile virus, West Nile virus NY99, West Nile virus WNV-1.7-3.3 mM, activation of ATPase activity to 170-180% of control656664 2D-image
Poly(U)Classical swine fever virus, Classical swine fever virus CSFV-the activation by poly(U) is inhibited by K+684774 2D-image
PolynucleotideVaccinia virus, Vaccinia virus Elstree-denatured DNA and RNA, synthetic RNA and DNA hybrids209880 2D-image
RNASaccharomyces cerevisiae-stimulates the ATP hydrolysis dependent on the chain length667694 2D-image
TaurineRattus norvegicus-activates the enzyme, and acts as antagonist and reduces the hypochlorous acid toxicity671005 2D-image
DTTDengue virus, Dengue virus DENV, Dengue virus PL046--670994 2D-image
additional informationSulfolobus solfataricus-the ATPase domain of Cdc6-2 retains to a great extent the origin DNA-binding activity, and likewise maintains its stimulating effect on Cdc6-3, the ATPase domain of Cdc6-1, which also stimulated the DNA-binding ability of Cdc6-3690616-
additional informationSulfolobus tokodaii, Sulfolobus tokodaii 7, Sulfolobus tokodaii SSO0016-Rad55B is induced by UV light. Rad55B has a very weak ATPase activity which is not stimulated by DNA695127-

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.8-2',3'-didehydro-thymidine triphosphateHepatitis C virus-pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity656494 2D-image
2.3-3'-azido-2',3'-dideoxythymidine triphosphateHepatitis C virus-pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity656494 2D-image
0.046-ADPHomo sapiens-pH 7.0, 22°C, recombinant wild-type enzyme688347 2D-image
0.217-ADPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.000028-ATPSaccharomyces cerevisiae-pH 7.5, 23°C, in presence of poly(A)40667694 2D-image
0.005-ATPHomo sapiens-pH 7.0, 22°C, recombinant wild-type enzyme688347 2D-image
0.0095-ATPWest Nile virus--656664 2D-image
0.108-ATPDengue virus-recombinant wild-type enzyme, pH 7.4, 30°C670994 2D-image
0.138-ATPSaccharomyces cerevisiae-pH 7.5, 23°C667694 2D-image
0.26-ATPRattus norvegicus--209878 2D-image
0.26-ATPBos taurus-in presence of 10 mM Mg2+696153 2D-image
0.317-ATPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.45-ATPHepatitis C virus-pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity656494 2D-image
0.54-ATPHepatitis C virus-pH 7.5, 25°C, NTPase activity656494 2D-image
0.7-ATPHuman enterovirus C, Human enterovirus C Mahoney, Human enterovirus C Sabin--209889 2D-image
2.5-ATPmammalian orthoreovirus, mammalian orthoreovirus mORV-wild-type transcriptase cofactor my2, 22°C, 6.5-7.0669274 2D-image
0.082-CDPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.5-CDPBos taurus-in presence of 10 mM Mg2+696153 2D-image
0.006-CTPHomo sapiens-pH 7.0, 22°C, recombinant wild-type enzyme688347 2D-image
0.034-CTPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.15-CTPBos taurus-in presence of 10 mM Mg2+696153 2D-image
0.239-CTPSaccharomyces cerevisiae-pH 7.5, 23°C667694 2D-image
3.8-CTPmammalian orthoreovirus, mammalian orthoreovirus mORV-wild-type transcriptase cofactor my2, 22°C, 6.5-7.0669274 2D-image
0.145-dADPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.062-dATPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.147-dATPSaccharomyces cerevisiae-pH 7.5, 23°C667694 2D-image
6.9-dATPHepatitis C virus-pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity656494 2D-image
0.092-dCDPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.023-dCTPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.179-dCTPSaccharomyces cerevisiae-pH 7.5, 23°C667694 2D-image
4-dCTPHepatitis C virus-pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity656494 2D-image
3.8-ddCTPHepatitis C virus-pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity656494 2D-image
0.9-ddTTPHepatitis C virus-pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity656494 2D-image
0.051-dGDPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.083-dGTPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.144-dGTPSaccharomyces cerevisiae-pH 7.5, 23°C667694 2D-image
0.088-dITPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.0000000047-DNA duplexWest Nile virus--656664-
0.04-dTTPHomo sapiens--209890 2D-image
0.147-dTTPSaccharomyces cerevisiae-pH 7.5, 23°C667694 2D-image
2.4-dTTPHepatitis C virus-pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity656494 2D-image
0.092-GDPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.004-GTPHomo sapiens-pH 7.0, 22°C, recombinant wild-type enzyme688347 2D-image
0.041-GTPBos taurus-in presence of 10 mM Mg2+696153 2D-image
0.132-GTPSaccharomyces cerevisiae-pH 7.5, 23°C667694 2D-image
0.394-GTPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
3.7-GTPmammalian orthoreovirus, mammalian orthoreovirus mORV-wild-type transcriptase cofactor my2, 22°C, 6.5-7.0669274 2D-image
0.046-IDPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.025-ITPBos taurus-in presence of 10 mM Mg2+696153 2D-image
0.147-ITPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.8-L-dATPHepatitis C virus-pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity656494 2D-image
9.9-L-dCTPHepatitis C virus-pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity656494 2D-image
28-L-dTTPHepatitis C virus-pH 7.5, 25°C, NTP hydrolysis-dependent helicase activity656494 2D-image
0.00032-RNAmammalian orthoreovirus, mammalian orthoreovirus mORV-wild-type transcriptase cofactor my2, 22°C, 6.5-7.0669274 2D-image
0.105-TDPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.007-TTPHomo sapiens-pH 7.0, 22°C, recombinant wild-type enzyme688347 2D-image
0.138-TTPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.137-UDPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.005-UTPHomo sapiens-pH 7.0, 22°C, recombinant wild-type enzyme688347 2D-image
0.086-UTPBos taurus-in presence of 10 mM Mg2+696153 2D-image
0.124-UTPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.297-UTPSaccharomyces cerevisiae-pH 7.5, 23°C667694 2D-image
9.4-UTPmammalian orthoreovirus, mammalian orthoreovirus mORV-wild-type transcriptase cofactor my2, 22°C, 6.5-7.0669274 2D-image
0.0185-MgATP2-Oryctolagus cuniculus-concentration of MgATP2- higher than 0.006 mM209874 2D-image
additional information-additional informationVaccinia virus, Vaccinia virus Elstree--209880-
additional information-additional informationRattus norvegicus--209881-
additional information-additional informationPisum sativum--209884, 209885-
additional information-additional informationSaccharomyces cerevisiae--667694-
additional information-additional informationDengue virus-kinetics670994-

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.0051-ADPHomo sapiens-pH 7.0, 22°C, recombinant wild-type enzyme688347 2D-image
0.29-ADPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.0086-ATPHomo sapiens-pH 7.0, 22°C, recombinant wild-type enzyme688347 2D-image
0.29-ATPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.93-ATPSaccharomyces cerevisiae-pH 7.5, 23°C667694 2D-image
1.4-ATPSouthampton virusQ04544-656663 2D-image
10.25-ATPSaccharomyces cerevisiae-pH 7.5, 23°C667694 2D-image
13.8-ATPmammalian orthoreovirus, mammalian orthoreovirus mORV-wild-type transcriptase cofactor my2, 22°C, 6.5-7.0669274 2D-image
133-ATPWest Nile virus-pH 7.5, 30°C656664 2D-image
155-ATPClassical swine fever virus-pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U)684774 2D-image
4384-ATPClassical swine fever virus-pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U)684774 2D-image
1.67-CDPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.0073-CTPHomo sapiens-pH 7.0, 22°C, recombinant wild-type enzyme688347 2D-image
0.59-CTPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.97-CTPSaccharomyces cerevisiae-pH 7.5, 23°C667694 2D-image
8-CTPmammalian orthoreovirus, mammalian orthoreovirus mORV-wild-type transcriptase cofactor my2, 22°C, 6.5-7.0669274 2D-image
16.67-CTPSaccharomyces cerevisiae-pH 7.5, 23°C667694 2D-image
220-CTPClassical swine fever virus, Classical swine fever virus CSFV-pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U)684774 2D-image
2286-CTPClassical swine fever virus-pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U)684774 2D-image
0.52-dADPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.0570.65dATPSaccharomyces cerevisiae-pH 7.5, 23°C667694 2D-image
0.31-dATPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
11.18-dATPSaccharomyces cerevisiae-pH 7.5, 23°C667694 2D-image
208-dATPClassical swine fever virus-pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U)684774 2D-image
3150-dATPClassical swine fever virus-pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U)684774 2D-image
1.12-dCDPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.3-dCTPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.5-dCTPSaccharomyces cerevisiae-pH 7.5, 23°C667694 2D-image
12.57-dCTPSaccharomyces cerevisiae-pH 7.5, 23°C667694 2D-image
135-dCTPClassical swine fever virus, Classical swine fever virus CSFV-pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U)684774 2D-image
5096-dCTPClassical swine fever virus-pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U)684774 2D-image
0.51-dGDPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.4-dGTPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
10.2-dGTPSaccharomyces cerevisiae-pH 7.5, 23°C667694 2D-image
136-dGTPClassical swine fever virus-pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U)684774 2D-image
2500-dGTPClassical swine fever virus, Classical swine fever virus CSFV-pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U)684774 2D-image
0.22-dITPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
10.9-dTTPSaccharomyces cerevisiae-pH 7.5, 23°C667694 2D-image
82-dUTPClassical swine fever virus, Classical swine fever virus CSFV-pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U)684774 2D-image
1875-dUTPClassical swine fever virus-pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U)684774 2D-image
0.39-GDPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.0076-GTPHomo sapiens-pH 7.0, 22°C, recombinant wild-type enzyme688347 2D-image
0.041.97GTPSaccharomyces cerevisiae-pH 7.5, 23°C667694 2D-image
0.32-GTPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
9.03-GTPSaccharomyces cerevisiae-pH 7.5, 23°C667694 2D-image
9.8-GTPmammalian orthoreovirus, mammalian orthoreovirus mORV-wild-type transcriptase cofactor my2, 22°C, 6.5-7.0669274 2D-image
400-GTPClassical swine fever virus, Classical swine fever virus CSFV-pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U)684774 2D-image
2963-GTPClassical swine fever virus-pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U)684774 2D-image
0.06-IDPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.005-RNAmammalian orthoreovirus, mammalian orthoreovirus mORV-wild-type transcriptase cofactor my2, 22°C, 6.5-7.0669274 2D-image
1.03-TDPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.0074-TTPHomo sapiens-pH 7.0, 22°C, recombinant wild-type enzyme688347 2D-image
0.69-TTPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
1.35-UDPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.007-UTPHomo sapiens-pH 7.0, 22°C, recombinant wild-type enzyme688347 2D-image
0.53-UTPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
0.97-UTPSaccharomyces cerevisiae-pH 7.5, 23°C667694 2D-image
7.3-UTPmammalian orthoreovirus, mammalian orthoreovirus mORV-wild-type transcriptase cofactor my2, 22°C, 6.5-7.0669274 2D-image
17.28-UTPSaccharomyces cerevisiae-pH 7.5, 23°C667694 2D-image
114-UTPClassical swine fever virus-pH 6.5, 37°C, recombinant truncated protein ntNS3, in absence of poly(U)684774 2D-image
2162-UTPClassical swine fever virus-pH 6.5, 37°C, recombinant truncated protein ntNS3, in presence of poly(U)684774 2D-image
0.18-ITPDeinococcus radioduransQ9RXX6substrate concentration between 0.01 and 4.0 mM, 37°C696275 2D-image
additional information-additional informationWest Nile virus-at optimum Mg2+ and saturating ATP concentrations 1 pmol of enzyme unwinds 5.5 fmol of DNA duplex per s656664-

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.6-2',3'-didehydro-thymidine triphosphateHepatitis C virus-pH 7.5, 25°C, inhibition of ATPase activity656494 2D-image
2.5-2',3'-dideoxy-5-fluorocytidine triphosphateHepatitis C virus-pH 7.5, 25°C, inhibition of ATPase activity656494 2D-image
2.1-3'-azido-2',3'-dideoxythymidine triphosphateHepatitis C virus-pH 7.5, 25°C, inhibition of ATPase activity656494 2D-image
0.54-ATPHepatitis C virus-pH 7.5, 25°C, inhibition of ATPase activity656494 2D-image
0.6-dATPHepatitis C virus-pH 7.5, 25°C, inhibition of ATPase activity656494 2D-image
3-dCTPHepatitis C virus-pH 7.5, 25°C, inhibition of ATPase activity656494 2D-image
3.8-ddCTPHepatitis C virus-pH 7.5, 25°C, inhibition of ATPase activity656494 2D-image
1.2-ddTTPHepatitis C virus-pH 7.5, 25°C, inhibition of ATPase activity656494 2D-image
3.5-dGTPHepatitis C virus-pH 7.5, 25°C, inhibition of ATPase activity656494 2D-image
2.8-dTTPHepatitis C virus-pH 7.5, 25°C, inhibition of ATPase activity656494 2D-image
1.2-L-dATPHepatitis C virus-pH 7.5, 25°C, inhibition of ATPase activity656494 2D-image
1.5-L-dCTPHepatitis C virus-pH 7.5, 25°C, inhibition of ATPase activity656494 2D-image
2.3-L-ddCTPHepatitis C virus-pH 7.5, 25°C, inhibition of ATPase activity656494 2D-image
1.3-L-dTTPHepatitis C virus-pH 7.5, 25°C, inhibition of ATPase activity656494 2D-image

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.075-5-fluoro-2-selenocytosineWest Nile virus-IC50: 0.075 mM for NTPase reaction, no influence to helicase activity up to a concentration of 0.5 mM656664 2D-image
0.12-ribavirin-TPWest Nile virus-at ATP concentration equal to Km, IC50 of NTPase reaction is 0.4 mM, classical competitive inhibitor with regard to ATP. At ATP and DNA duplex concentrations corresponding to their KM-values an IC50 of 0.12 mM is measured. Inhibition reaches a maximum of656664 2D-image

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
0.00008-Pseudomonas aeruginosa-PilU688871
0.00012-Pseudomonas aeruginosa-PilT688871
0.00018-Pseudomonas aeruginosa-PilB688871
1.9-Rattus norvegicus--209881
25.4-Rattus norvegicus--209876
160.8-West Nile virus, West Nile virus NY99, West Nile virus WNV--656664
additional information-Rattus norvegicus--209881
additional information-potato virus A, potato virus X--668616
additional information-Dengue virus DENV, Dengue virus PL046, Dengue virus-the RNA helicase, nucleotide 5'-triphosphatase, and RNA 5'-triphosphatase activities of wild-type andmutant enzymes, overview670994
additional information-Classical swine fever virus, Classical swine fever virus CSFV--684774
additional information-Pseudomonas aeruginosa-activity of mutant enzymes, overview688871
additional information-Bos taurus-dependence on bivalent cations and broad substrate specificity towards nucleoside-5'-tri- and -diphosphates reveal similarities to apyrases696153
additional information-Deinococcus radioduransQ9RXX6identification of residues involved in metal and substrate binding using NMR chemical shift mapping experiments, results mapped onto the crystal structure, substrate binding possibilities and biological function discussed, further biochemical studies necessary to determine if the specificity for CDP and CTP is part of its housekeeping duties696275

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
3-Homo sapiens--209890
5.5-Sulfolobus solfataricus-ATPase assay at694432
69Classical swine fever virus-broad range optimum, pH profile684774
6.57mammalian orthoreovirus--669274
7-Homo sapiens-assay at688347
7-Sulfolobus tokodaii-DNA repair aasay at695127
7.27.5Southampton virusQ04544ATPase activity656663
7.4-Dengue virus-ATPase assay at670994
7.5-Dengue virus, Hepatitis C virus, Japanese encephalitis virus, West Nile virus-assay at667226
7.5-Saccharomyces cerevisiae-ssay at667694
7.5-Dengue virus-helicase assay at670994
7.5-Pseudomonas aeruginosa-assay at688871
7.5-Sulfolobus tokodaii-ATPase assay at695127
7.5-Bos taurus--696153
88.2Rattus norvegicus--209881
8.5-Bacillus subtilis-assay at669092
8.6-Rattus norvegicus--209876
9-Deinococcus radioduransQ9RXX6hydrolysis of CDP696275

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
5.59Classical swine fever virus-pH profile684774
5.79mammalian orthoreovirus-50% of maximal activity at pH 5.7, and pH 9.0669274
68.5Bos taurus-pH 6.0: about 55% of maximal activity, pH 8.5: about 55% of maximal activity696153
6.88Southampton virusQ04544pH 6.8: about 90% of maximal activity, pH 8.0: about 70% of maximal activity656663
99.5Deinococcus radioduransQ9RXX6optimal range for hydrolysis of CDP between696275

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
15-Southampton virusQ04544-656663
22-mammalian orthoreovirus, mammalian orthoreovirus mORV-assay at room temperature669274
22-Homo sapiens-assay at room temperature688347
23-Saccharomyces cerevisiae-assay at667694
24-Pseudomonas aeruginosa-assay at688871
25-Dengue virus DENV, Dengue virus, Dengue virus PL046-helicase assay at670994
30-Dengue virus, Dengue virus DENV, Dengue virus PL046-ATPase assay at670994
30-Rattus norvegicus-assay at671005
34-Rattus norvegicus--209876
37-Bacillus subtilis-assay at669092
37-Classical swine fever virus, Classical swine fever virus CSFV-assay at684774
37-Bos taurus-assay at696153
44-Rattus norvegicus--209881
44-Homo sapiens--209890
60-Sulfolobus solfataricus-ATPase assay at694432
60-Sulfolobus tokodaii-ATPase assay at695127

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
2044Homo sapiens--209890

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
brainBos taurus-purified from696153Manually annotated by BRENDA team
culture mediumWest Nile virus, West Nile virus NY99, West Nile virus WNV-from virus-infected Vero cells656664Manually annotated by BRENDA team
germ cellMus musculus--667903Manually annotated by BRENDA team
heartBos taurus-expression in696153Manually annotated by BRENDA team
kidneyBos taurus-expression in696153Manually annotated by BRENDA team
liverRattus norvegicus--209876, 209878, 671005Manually annotated by BRENDA team
liverBos taurus-expression in696153Manually annotated by BRENDA team
lungBos taurus-expression in696153Manually annotated by BRENDA team
neuronHomo sapiens, Rattus norvegicus--668745Manually annotated by BRENDA team
ovaryMus musculus-expression in intermediary, primary, and secondary follicles667903Manually annotated by BRENDA team
pancreasRattus norvegicus--209881Manually annotated by BRENDA team
serumHomo sapiens--209890Manually annotated by BRENDA team
skeletal muscleOryctolagus cuniculus--209874Manually annotated by BRENDA team
small intestineBos taurus-expression in696153Manually annotated by BRENDA team
spleenBos taurus-expression in696153Manually annotated by BRENDA team
mesenteric arteryRattus norvegicus-small mesenteric artery209886Manually annotated by BRENDA team
additional informationSouthampton virusQ04544viral protein is expressed in Escherichia coli cells656663Manually annotated by BRENDA team
additional informationWest Nile virus, West Nile virus NY99, West Nile virus WNV-virus from Vero E6 cell culture, excreted to the medium667226Manually annotated by BRENDA team
additional informationMus musculus-expression pattern of NALP14, overview667903Manually annotated by BRENDA team
additional informationmammalian orthoreovirus, mammalian orthoreovirus mORV-the transcriptase cofactor my2 is associated to microtubules in host cells, overview669274Manually annotated by BRENDA team

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
cytosolBos taurus--5829696153Manually annotated by BRENDA team
membranePisum sativum--16020209883, 209884Manually annotated by BRENDA team
membraneRattus norvegicus--16020209881, 209886Manually annotated by BRENDA team
membraneAgrobacterium tumefaciens, Agrobacterium tumefaciens 30147, Agrobacterium tumefaciens 30150, Agrobacterium tumefaciens 47C, Agrobacterium tumefaciens AD1, Agrobacterium tumefaciens AKU, Agrobacterium tumefaciens AKU 0300, Agrobacterium tumefaciens AR50, Agrobacterium tumefaciens ATCC 15955, Agrobacterium tumefaciens B-261, Agrobacterium tumefaciens B3/73, Agrobacterium tumefaciens B6-6, Agrobacterium tumefaciens BQL9, Agrobacterium tumefaciens CCRC 14924, Agrobacterium tumefaciens CIP 67.1, Agrobacterium tumefaciens D3, Agrobacterium tumefaciens DUL-DH101, Agrobacterium tumefaciens GV4R10c21, Agrobacterium tumefaciens IAM13299, Agrobacterium tumefaciens IAM 1525, Agrobacterium tumefaciens IAM 1526, Agrobacterium tumefaciens IAM B-261, Agrobacterium tumefaciens ICR, Agrobacterium tumefaciens J14a, Agrobacterium tumefaciens K-28, Agrobacterium tumefaciens K84, Agrobacterium tumefaciens KCCM 10413, Agrobacterium tumefaciens M-1, Agrobacterium tumefaciens NRRL, Agrobacterium tumefaciens NRRL B11291, Agrobacterium tumefaciens NTRRL B11291, Agrobacterium tumefaciens R10, Agrobacterium tumefaciens RS5, Agrobacterium tumefaciens S2, Agrobacterium tumefaciens S33, Agrobacterium tumefaciens ST96-4, Agrobacterium tumefaciens YH-2-transmembrane protein16020668745Manually annotated by BRENDA team
membraneAnabaena sp., Anabaena sp. 7119, Anabaena sp. 7120, Anabaena sp. PCC6301, Anabaena sp. PCC7119, Anabaena sp. PCC 7120, Anabaena sp. PCC7120, Caenorhabditis elegans-the enzyme contains two membrane-spanning helices inserted into the N-terminal Walker B motif16020668745Manually annotated by BRENDA team
membraneClostridium perfringens, Corynebacterium efficiens, Corynebacterium glutamicum--16020668745Manually annotated by BRENDA team
membraneCupriavidus metallidurans, Deinococcus radiodurans, Deinococcus radiodurans KR1, Deinococcus radiodurans RI-transmembrane protein16020668745Manually annotated by BRENDA team
membraneDrosophila melanogaster-the enzyme contains two membrane-spanning helices inserted into the N-terminal Walker B motif16020668745Manually annotated by BRENDA team
membraneEscherichia coli-transmembrane protein16020668745Manually annotated by BRENDA team
membraneGeobacter sulfurreducens, Geobacter sulfurreducens DL-1, Geobacter sulfurreducens DL1, Homo sapiens-the enzyme contains two membrane-spanning helices inserted into the N-terminal Walker B motif16020668745Manually annotated by BRENDA team
membraneKlebsiella pneumoniae, Leuconostoc mesenteroides, Leuconostoc mesenteroides 0326, Leuconostoc mesenteroides AKU 1102, Leuconostoc mesenteroides ATCC8293, Leuconostoc mesenteroides B-1149, Leuconostoc mesenteroides B-1299, Leuconostoc mesenteroides B-1299CB, Leuconostoc mesenteroides B-1299CB4, Leuconostoc mesenteroides B-1375, Leuconostoc mesenteroides B-512F, Leuconostoc mesenteroides B-512FM, Leuconostoc mesenteroides B-512FMC, Leuconostoc mesenteroides B-512 FMC, Leuconostoc mesenteroides B-512FMCM, Leuconostoc mesenteroides B-742CB, Leuconostoc mesenteroides Birmingham, Leuconostoc mesenteroides CGMCC 1.544, Leuconostoc mesenteroides DBPZ0985, Leuconostoc mesenteroides FT 045 B, Leuconostoc mesenteroides FT045B, Leuconostoc mesenteroides IAM 1046, Leuconostoc mesenteroides IBT-PQ, Leuconostoc mesenteroides IFO 3426, Leuconostoc mesenteroides JK55, Leuconostoc mesenteroides KIBGE IB-22, Leuconostoc mesenteroides LM-0326, Leuconostoc mesenteroides Lm 28, Leuconostoc mesenteroides NRRL B-1299, Leuconostoc mesenteroides NRRL B-1355, Leuconostoc mesenteroides NRRL B-1416, Leuconostoc mesenteroides NRRL B-21297, Leuconostoc mesenteroides NRRL B-23192, Leuconostoc mesenteroides NRRL B-512F, Leuconostoc mesenteroides NRRl B-512(F), Leuconostoc mesenteroides NRRL B512-F, Leuconostoc mesenteroides NRRL B-640, Leuconostoc mesenteroides NRRL B-742, Leuconostoc mesenteroides P-60, Leuconostoc mesenteroides PCSIR-4, Leuconostoc mesenteroides Sikhae--16020668745Manually annotated by BRENDA team
membraneMagnetococcus sp., Magnetococcus sp. mc-1-transmembrane protein16020668745Manually annotated by BRENDA team
membraneNostoc punctiforme, Nostoc punctiforme IAM M-15, Nostoc punctiforme PCC73102, Pseudomonas putida--16020668745Manually annotated by BRENDA team
membraneRattus norvegicus, Saccharophagus degradans, Saccharophagus degradans 2-40T-the enzyme contains two membrane-spanning helices inserted into the N-terminal Walker B motif16020668745Manually annotated by BRENDA team
membraneStaphylococcus epidermidis, Staphylococcus epidermidis 14990, Staphylococcus epidermidis ATCC, Staphylococcus epidermidis ATCC12228, Staphylococcus epidermidis ATCC 14990, Staphylococcus epidermidis RYC 13036, Staphylococcus epidermidis Texas 26, Synechocystis sp., Thermotoga maritima, Vibrio parahaemolyticus, Vibrio parahaemolyticus AQ 3627, Vibrio parahaemolyticus EB101, Vibrio parahaemolyticus KN1699, Vibrio parahaemolyticus RIMD2210633, Vibrio parahaemolyticus Y-4, Vibrio vulnificus, Vibrio vulnificus CYK279H, Vibrio vulnificus MO6-24/O--16020668745Manually annotated by BRENDA team
membraneDeinococcus radioduransQ9RXX6-16020696275Manually annotated by BRENDA team
nuclear envelopeRattus norvegicus--5635209876Manually annotated by BRENDA team
nucleusRattus norvegicus-nuclear envelope5634671005Manually annotated by BRENDA team
sarcoplasmic reticulumOryctolagus cuniculus--16529209874Manually annotated by BRENDA team

PDBSCOPCATHORGANISM
3l6p, downloadSCOP (3l6p)CATH (3l6p)Dengue virus 1
3lkw, downloadSCOP (3lkw)CATH (3lkw)Dengue virus 1
3u1i, downloadSCOP (3u1i)CATH (3u1i)Dengue virus type 3 (strain Singapore/8120/1995)
3u1j, downloadSCOP (3u1j)CATH (3u1j)Dengue virus type 3 (strain Singapore/8120/1995)
2whx, downloadSCOP (2whx)CATH (2whx)Dengue virus type 4 (strain Thailand/0348/1991)
4a92, downloadSCOP (4a92)CATH (4a92)Hepatitis C virus genotype 1b (isolate BK)
4b6e, downloadSCOP (4b6e)CATH (4b6e)Hepatitis C virus genotype 1b (isolate BK)
4b6f, downloadSCOP (4b6f)CATH (4b6f)Hepatitis C virus genotype 1b (isolate BK)
4b71, downloadSCOP (4b71)CATH (4b71)Hepatitis C virus genotype 1b (isolate BK)
4b73, downloadSCOP (4b73)CATH (4b73)Hepatitis C virus genotype 1b (isolate BK)
4b74, downloadSCOP (4b74)CATH (4b74)Hepatitis C virus genotype 1b (isolate BK)
4b75, downloadSCOP (4b75)CATH (4b75)Hepatitis C virus genotype 1b (isolate BK)
4b76, downloadSCOP (4b76)CATH (4b76)Hepatitis C virus genotype 1b (isolate BK)
3kqh, downloadSCOP (3kqh)CATH (3kqh)Hepatitis C virus genotype 1b (isolate Con1)
3kqk, downloadSCOP (3kqk)CATH (3kqk)Hepatitis C virus genotype 1b (isolate Con1)
3kql, downloadSCOP (3kql)CATH (3kql)Hepatitis C virus genotype 1b (isolate Con1)
3kqn, downloadSCOP (3kqn)CATH (3kqn)Hepatitis C virus genotype 1b (isolate Con1)
3kqu, downloadSCOP (3kqu)CATH (3kqu)Hepatitis C virus genotype 1b (isolate Con1)
3oyp, downloadSCOP (3oyp)CATH (3oyp)Hepatitis C virus genotype 1b (isolate Japanese)
4a1t, downloadSCOP (4a1t)CATH (4a1t)Hepatitis C virus genotype 1b (isolate Japanese)
4a1v, downloadSCOP (4a1v)CATH (4a1v)Hepatitis C virus genotype 1b (isolate Japanese)
4a1x, downloadSCOP (4a1x)CATH (4a1x)Hepatitis C virus genotype 1b (isolate Japanese)
4i31, downloadSCOP (4i31)CATH (4i31)Hepatitis C virus genotype 1b (isolate Japanese)
4i32, downloadSCOP (4i32)CATH (4i32)Hepatitis C virus genotype 1b (isolate Japanese)
4i33, downloadSCOP (4i33)CATH (4i33)Hepatitis C virus genotype 1b (isolate Japanese)
4j02, downloadSCOP (4j02)CATH (4j02)Hepatitis C virus genotype 1b (strain HC-J4)
4j04, downloadSCOP (4j04)CATH (4j04)Hepatitis C virus genotype 1b (strain HC-J4)
4j06, downloadSCOP (4j06)CATH (4j06)Hepatitis C virus genotype 1b (strain HC-J4)
4j08, downloadSCOP (4j08)CATH (4j08)Hepatitis C virus genotype 1b (strain HC-J4)
4j0a, downloadSCOP (4j0a)CATH (4j0a)Hepatitis C virus genotype 1b (strain HC-J4)
3o8b, downloadSCOP (3o8b)CATH (3o8b)Hepatitis C virus subtype 1b
3o8c, downloadSCOP (3o8c)CATH (3o8c)Hepatitis C virus subtype 1b
3o8d, downloadSCOP (3o8d)CATH (3o8d)Hepatitis C virus subtype 1b
3o8r, downloadSCOP (3o8r)CATH (3o8r)Hepatitis C virus subtype 1b
2i3b, downloadSCOP (2i3b)CATH (2i3b)Homo sapiens
3agr, downloadSCOP (3agr)CATH (3agr)Neospora caninum
4imz, downloadSCOP (4imz)CATH (4imz)Norwalk virus (strain GI/Human/United States/Norwalk/1968)
4inh, downloadSCOP (4inh)CATH (4inh)Norwalk virus (strain GI/Human/United States/Norwalk/1968)
3iyb, downloadSCOP (3iyb)CATH (3iyb)Poliovirus type 1 (strain Mahoney)
3iyc, downloadSCOP (3iyc)CATH (3iyc)Poliovirus type 1 (strain Mahoney)
3iyb, downloadSCOP (3iyb)CATH (3iyb)Poliovirus type 3 (strains P3/Leon/37 and P3/Leon 12A[1]B)
3iyc, downloadSCOP (3iyc)CATH (3iyc)Poliovirus type 3 (strains P3/Leon/37 and P3/Leon 12A[1]B)
4gua, downloadSCOP (4gua)CATH (4gua)Sindbis virus
3s86, downloadSCOP (3s86)CATH (3s86)Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
4a57, downloadSCOP (4a57)CATH (4a57)Toxoplasma gondii
4jep, downloadSCOP (4jep)CATH (4jep)Toxoplasma gondii
2yol, downloadSCOP (2yol)CATH (2yol)West Nile virus

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
40000-Rattus norvegicus-SDS-PAGE209876
46000-Rattus norvegicus-SDS-PAGE209878
58600-Bos taurus-gel filtration, at 4°C in 20 mM Tris-HCl, 0.1 M NaCl, pH 7.5696153
60200-Bos taurus-SDS-PAGE696153
75000-Homo sapiens-gel filtration209890

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
?West Nile virus NY99, West Nile virus WNV, West Nile virus-x * 60000, SDS-PAGE656664
?Classical swine fever virus CSFV, Classical swine fever virus-x * 60000, recombinant full-length NS3 protein, SDS-PAGE684774
?Homo sapiens-x * 20500, recombinant enzyme, SDS-PAGE688347
?Bos taurus-x * 60200, SDS-PAGE696153
additional informationMus musculus-the enzyme contains a leucine-rich repeat-replete, LRR-replete, conserved in NACHT NTPases, three-dimensional structure667903
additional informationAgrobacterium tumefaciens, Anabaena sp. 7119, Anabaena sp. 7120, Anabaena sp. PCC6301, Anabaena sp. PCC7119, Anabaena sp. PCC 7120, Anabaena sp. PCC7120, Anabaena sp., Caenorhabditis elegans, Clostridium perfringens, Corynebacterium efficiens, Corynebacterium glutamicum, Cupriavidus metallidurans, Deinococcus radiodurans, Drosophila melanogaster, Escherichia coli, Geobacter sulfurreducens DL-1, Geobacter sulfurreducens DL1, Geobacter sulfurreducens, Homo sapiens, Klebsiella pneumoniae, Leuconostoc mesenteroides, Magnetococcus sp. mc-1, Magnetococcus sp., Nostoc punctiforme IAM M-15, Nostoc punctiforme PCC73102, Nostoc punctiforme, Pseudomonas putida, Rattus norvegicus, Saccharophagus degradans 2-40T, Saccharophagus degradans, Staphylococcus epidermidis 14990, Staphylococcus epidermidis ATCC12228, Staphylococcus epidermidis ATCC 14990, Staphylococcus epidermidis ATCC, Staphylococcus epidermidis RYC 13036, Staphylococcus epidermidis Texas 26, Staphylococcus epidermidis, Synechocystis sp., Thermotoga maritima, Vibrio parahaemolyticus, Vibrio vulnificus CYK279H, Vibrio vulnificus MO6-24/O, Vibrio vulnificus-the enzyme has the conserved P-loop NTPase fold, amino acid sequence alignment and structure comparison of KAP NTPases, overview668745
additional informationHuman coronavirus 229E-the enzyme contains an N-terminal zinc binding domain and a C-terminal superfamily 1 helicase domain670074
additional informationDengue virus DENV, Dengue virus PL046, Dengue virus-RNA helicase, nucleotide 5'-triphosphatase, and RNA 5'-triphosphatase activities are located on the C-terminal 54-kDa domain, the RTPase activity requuires an intact Walker B motif in the helicase core670994
additional informationHomo sapiens-three-dimensional structure determination by NMR and MALDI-TOF mass spectrometry, and structure analysis. The structure of the protein in its complex with ATPgammaS forms a three-layered alpha/beta sandwich, with a central nine-stranded beta-sheet surrounded by five alpha-helices. Sequence and three-dimensional structure comparisons with AAA+ ATPases revealed the presence of Walker A, GPPGVGKT, and Walker B, VCVIDEIG, motifs, overview688347
additional informationPseudomonas aeruginosa-the type IV pilus motor proteins contain conserved residues in the Walker A, Walker B, and Asp Box and His Box motifs characteristic of secretion NTPases, overview688871

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
determined in the metal-free form at 1.9 A resolution, data collection and structure refinement statistics, molscript ribbon representation of the crystal structureDeinococcus radioduransQ9RXX6696275
sitting drop vapor diffusion method, using 8-10% (w/v) PEG 3350, 0.1 M Tris-HCl pH 7.4, 50 mM diammonium phosphate and 2%(v/v) glycerol, at 20°CNeospora caninumC9K7J7718494
sitting drop vapor diffusion method, using 10-12% (w/v) PEG 3350, 0.1 M Tris-HCl pH 7.4, 2% (v/v) glycerol, at 20°CToxoplasma gondiiQ27893718494

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
No entries in this field

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-20°C, 3 months, 10% loss of activityRattus norvegicus-209876

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
recombinant enzyme from Escherichia coli strain HMS174 by streptomycin sulfate treatment and gel filtrationBacillus subtilis-669092
gel filtration, SDS-PAGEBos taurus-696153
recombinant His-tagged full-length viral NS3 protein and N-terminal truncated version ntNS3 from Escherichia coli by nickel affinity chromatography and dialysisClassical swine fever virus, Classical swine fever virus CSFV-684774
recombinant enzyme from Escherichia coliDengue virus-667226
recombinat His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtrationDengue virus-670994
recombinant enzyme from Escherichia coliDengue virus DENV, Dengue virus PL046-667226
recombinant NS3 from Escherichia coliHepatitis C virus-667226
recombinant wild-type and mutant enzymes from Escherichia coli by cation exchange chromatography and gel filtration to homogeneityHomo sapiens-688347
recombinant His-tagged enzyme from insect cellsHuman coronavirus 229E-670074
-Human enterovirus C, Human enterovirus C Mahoney, Human enterovirus C Sabin-209889
recombinant enzyme from Escherichia coliJapanese encephalitis virus, Japanese encephalitis virus JaOH0566, Japanese encephalitis virus JEV-667226
transcriptase cofactor my2 from host nucleoplasma by ion exchange and adsorption chromatography at room temperaturemammalian orthoreovirus, mammalian orthoreovirus mORV-669274
Toyopearl DEAE-650S column chromatography, Source 15Q column chromatography, and Superdex 200 pg gel filtrationNeospora caninumC9K7J7718494
-Oryctolagus cuniculus-209874
-Pisum sativum-209883, 209884, 209885
recombinant His6-tagged coat protein from Escherichia coli by nickel affinity chromatogryphypotato virus A, potato virus X-668616
recombinant His6-tagged wild-type and mutant enzymes from Escherichia coli strain Bl21 by nickel affinity chromatographyPseudomonas aeruginosa-688871
-Rattus norvegicus-209878
partialRattus norvegicus-209876
partially, isolation of hepatic nucleiRattus norvegicus-671005
recombinant His-tagged DEAH-box Prp22 protein from Escherichia coli strain BL21(DE3) by nickel affinity chromatography to homogeneitySaccharomyces cerevisiae-667694
-Southampton virusQ04544656663
recombinant GST-tagged Sso0909 from Escherichia coli by glutathione affinity chromatography and gel filtration, recombinant His10-tagged Sso0909 from Sulfolobus solfataricus by nickel affinity chromatography and gel filtration to homogeneitySulfolobus solfataricusQ97ZJ7690865
Toyopearl DEAE-650S column chromatography, Source 15Q column chromatography, and Superdex 200 pg gel filtrationToxoplasma gondiiQ27893718494
phosphohydrolase IIVaccinia virus-209880
-West Nile virus-656664
native enzyme from virus Vero E6 cell culture medium by affinity chromatography, ultrafiltration, and gel filtrationWest Nile virus-667226

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
phylogenetic tree and analysis of KAP NTPases, overviewAgrobacterium tumefaciens, Anabaena sp., Anabaena sp. 7119, Anabaena sp. 7120, Anabaena sp. PCC 7120, Anabaena sp. PCC6301, Anabaena sp. PCC7119, Anabaena sp. PCC7120-668745
gene ytkD, expression in Escherichia coli strain HMS174, complementation of the mutT mutator phenotype in Escherichia coli strain SB3 lacking a functional mutT geneBacillus subtilis-669092
phylogenetic tree and analysis of KAP NTPases, overviewCaenorhabditis elegans-668745
DNA and amino acid sequence determination and analysis, functional expression of His-tagged full-length viral NS3 protein, i.e. NS3FL, and its N-terminal truncated version ntNS3 in Escherichia coliClassical swine fever virus, Classical swine fever virus CSFV-684774
phylogenetic tree and analysis of KAP NTPases, overviewClostridium perfringens, Corynebacterium efficiens, Corynebacterium glutamicum, Cupriavidus metallidurans, Deinococcus radiodurans-668745
enzyme expression in Escherichia coliDengue virus-667226
expression of His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)Dengue virus-670994
phylogenetic tree and analysis of KAP NTPases, overviewDrosophila melanogaster, Escherichia coli, Geobacter sulfurreducens, Geobacter sulfurreducens DL-1, Geobacter sulfurreducens DL1-668745
expression of NS3 in Escherichia coliHepatitis C virus-667226
DNA and amino acid sequence determination and analysis, expression of wild-type and mutant enzymes in Escherichia coliHomo sapiens-688347
phylogenetic tree and analysis of KAP NTPases, overviewHomo sapiens-668745
nsp13, expression of the His-tagged enzyme in insect cellsHuman coronavirus 229E-670074
expression as glutathione S-transferase fusion protein in Escherichia coliHuman enterovirus C, Human enterovirus C Mahoney, Human enterovirus C Sabin-209889
enzyme expression in Escherichia coliJapanese encephalitis virus, Japanese encephalitis virus JaOH0566, Japanese encephalitis virus JEV-667226
phylogenetic tree and analysis of KAP NTPases, overviewKlebsiella pneumoniae, Leuconostoc mesenteroides, Magnetococcus sp., Magnetococcus sp. mc-1-668745
gene M1, expression of transcriptase cofactor my2 mutant K415A/K419A in Spodoptera frugiperda Sf21 insect cells using the baculovirus systemmammalian orthoreovirus, mammalian orthoreovirus mORV-669274
gene Nalp14, DNA and amino acid sequence determination and analysis, genomic organization and allelic representation, expression analysis, overview, expression of NALP14 in Escherichia coli strain XL-2 BlueMus musculus-667903
expressed in Escherichia coli BL21 cellsNeospora caninumC9K7J7718494
phylogenetic tree and analysis of KAP NTPases, overviewNostoc punctiforme, Nostoc punctiforme IAM M-15, Nostoc punctiforme PCC73102-668745
functional overexpression of the His6-tagged coat protein in Escherichia colipotato virus A, potato virus X-668616
genes pilB, pilT and pilU, DNA and amino acid sequence determination and anaylsis, expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain Bl21Pseudomonas aeruginosa-688871
phylogenetic tree and analysis of KAP NTPases, overviewPseudomonas putida, Rattus norvegicus-668745
expression of His-tagged DEAH-box Prp22 protein in Escherichia coli strain BL21(DE3)Saccharomyces cerevisiae-667694
phylogenetic tree and analysis of KAP NTPases, overviewSaccharophagus degradans, Saccharophagus degradans 2-40T, Staphylococcus epidermidis-668745
gene sso0909, DNA and amino acid sequence determination and analysis, expression analysis, sequence comparisons, phylogenetic analysis, overview. Expression of GST-tagged Sso0909 in Escherichia coli, overexpression of His10-tagged Sso0909 in Sulfolobus solfataricusSulfolobus solfataricusQ97ZJ7690865
expression of untagged Rad55B in Escherichia coli strain BL21(DE3)Sulfolobus tokodaii-695127
phylogenetic tree and analysis of KAP NTPases, overviewSynechocystis sp., Thermotoga maritima-668745
expressed in Escherichia coli BL21 cellsToxoplasma gondiiQ27893718494
phylogenetic tree and analysis of KAP NTPases, overviewVibrio parahaemolyticus, Vibrio parahaemolyticus AQ 3627, Vibrio parahaemolyticus EB101, Vibrio parahaemolyticus KN1699, Vibrio parahaemolyticus RIMD2210633, Vibrio parahaemolyticus Y-4, Vibrio vulnificus, Vibrio vulnificus CYK279H, Vibrio vulnificus MO6-24/O-668745

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
A354VClassical swine fever virus-site-directed mutagenesis of the motif III residue does not affect the enzyme activity and kinetics684774
K232AClassical swine fever virus-site-directed mutagenesis of the conserved motif I residue leads to inactivation of the mutant684774
A354VClassical swine fever virus CSFV-site-directed mutagenesis of the motif III residue does not affect the enzyme activity and kinetics684774
K232AClassical swine fever virus CSFV-site-directed mutagenesis of the conserved motif I residue leads to inactivation of the mutant684774
D284ADengue virus-site-directed mutagenesis, mutation in the Walker B motif, inactive mutant670994
E285ADengue virus-site-directed mutagenesis, mutation in the Walker B motif, the mutant does not show RTPase and NTPase activities, and is unable to remove gamma-phosphate from substrate pppAC5670994
E578ADengue virus-site-directed mutagenesis, mutation of a highly conserved residue, the mutant retains full NTPase, RTPase and helicase activities670994
R513ADengue virus-site-directed mutagenesis, mutation in the C-terminal domain of DELTANS3, the mutation reduces NTPase activity, abrogates helicase activity and strongly diminishes RTPase activity670994
E113THomo sapiens-site-directed mutagenesis, the mutation in the NTP binding site has a significant effect on the hydrolysis rate of the purine nucleotides, but has only a small effect on the protein structure688347
G115HHomo sapiens-site-directed mutagenesis, the mutation in the NTP binding site has a significant effect on the hydrolysis rate of the purine nucleotides, but has only a small effect on the protein structure688347
D160NPseudomonas aeruginosa-site-directed mutagenesis, mutation of the Asp Box motif residue in PilT688871
E159QPseudomonas aeruginosa-site-directed mutagenesis, mutation of the Asp Box motif residue in PilT688871
E163QPseudomonas aeruginosa-site-directed mutagenesis, mutation of the Asp Box motif residue in PilT688871
E204QPseudomonas aeruginosa-site-directed mutagenesis, mutation of the Walker B motif residue in PilT688871
G135SPseudomonas aeruginosa-site-directed mutagenesis, mutation of the Walker A motif residue in PilT688871
H222APseudomonas aeruginosa-site-directed mutagenesis, mutation of the His Box motif residue in PilT688871
C466SSulfolobus solfataricus-site-directed mutagenesis leading to increased formation of protein aggregates694432
C499SSulfolobus solfataricus-site-directed mutagenesis leading to increased formation of protein aggregates694432
K199ADengue virus-site-directed mutagenesis, mutation in the Walker B motif, the mutant does not show RTPase and NTPase activities, and is unable to remove gamma-phosphate from substrate pppAC5670994
additional informationDengue virus-construction of mutant DELTANS3 comprising the C-terminal 54-kDa domain of NS3, amino acid residues 169-619670994
K415A/K419Amammalian orthoreovirus, mammalian orthoreovirus mORV-site-directed mutagenesis, mutation of residues in the nucleotide binding domain of transcriptase cofactor my2, leads to loss of enzyme activity669274
additional informationpotato virus X-virus C-terminal regions deletion diminishes the ATPase activity668616
H229APseudomonas aeruginosa-site-directed mutagenesis, mutation of the His Box motif residue in PilT688871
additional informationPseudomonas aeruginosa-mutation of conserved Walker A or Walker B residues in any of the three ATPases abrogates twitching motility, and for the Walker A mutant of PilT causes loss of polar localization, construction of diverse mutants of the type IV pilus motor proteins, overview688871
C670SSulfolobus solfataricus-site-directed mutagenesis leading to increased formation of protein aggregates694432
additional informationSulfolobus solfataricusQ97ZJ7targeted gene disruption of gene sso0909 via genetic recombination, mutant phenotype, overview690865

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
medicineHepatitis C virus-considered attractive target for anti-hepatitis C virus chemoterapy656494

DISEASETITLE OF PUBLICATIONLINK TO PUBMED
African Horse Sickness- PubMed,  PubMed,  PubMed,  PubMed
African Swine Fever- PubMed,  PubMed
Alzheimer Disease- PubMed
Arthritis, Rheumatoid- PubMed
Astrocytoma- PubMed
Azoospermia- PubMed
Bluetongue- PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed
Brain Ischemia- PubMed
Breast Neoplasms- PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed
Carcinoma- PubMed,  PubMed,  PubMed,  PubMed
Carcinoma, Hepatocellular- PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed
Carcinoma, Small Cell- PubMed
Cardiovascular Abnormalities- PubMed
Cell Transformation, Neoplastic- PubMed,  PubMed,  PubMed
Classical Swine Fever- PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed
Coinfection- PubMed
Coronavirus Infections- PubMed
Cysts- PubMed,  PubMed
Dementia- PubMed
Dengue- PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed
Encephalitis- PubMed
Encephalitis, Japanese- PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed
Encephalitis, Tick-Borne- PubMed,  PubMed,  PubMed,  PubMed,  PubMed
Encephalitis, Viral- PubMed
Encephalomyelitis, Western Equine- PubMed
Fanconi Anemia- PubMed
Flavivirus Infections- PubMed
Foot-and-Mouth Disease- PubMed
Fowlpox- PubMed
Hepatitis- PubMed,  PubMed,  PubMed,  PubMed,  PubMed
Hepatitis A- PubMed
Hepatitis C- PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed
Hepatitis C, Chronic- PubMed,  PubMed,  PubMed,  PubMed
Hepatitis E- PubMed
Herpes Simplex- PubMed,  PubMed,  PubMed
Hypercholesterolemia- PubMed
Hyperlipidemias- PubMed
Hypersensitivity- PubMed,  PubMed
Hypertriglyceridemia- PubMed
hypoxanthine phosphoribosyltransferase deficiency- PubMed
Infection- PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed
Influenza, Human- PubMed,  PubMed
Intellectual Disability- PubMed
Lesch-Nyhan Syndrome- PubMed
Lymphoma- PubMed,  PubMed,  PubMed
Melanoma- PubMed,  PubMed,  PubMed,  PubMed
Mental Retardation, X-Linked- PubMed
Mouth Diseases- PubMed
Neoplasm Metastasis- PubMed
Neoplasms- PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed
Neuroblastoma- PubMed,  PubMed,  PubMed
Neurodegenerative Diseases- PubMed
Neurofibroma- PubMed
non-specific serine/threonine protein kinase deficiency- PubMed
nucleoside-triphosphatase deficiency- PubMed,  PubMed
Oligospermia- PubMed
Osteoarthritis- PubMed
Parathyroid Neoplasms- PubMed
Parkinson Disease- PubMed
Polycystic Kidney Diseases- PubMed
Rubella- PubMed,  PubMed
Sarcoma- PubMed
Severe Acute Respiratory Syndrome- PubMed,  PubMed,  PubMed,  PubMed
Starvation- PubMed,  PubMed
Telangiectasis- PubMed
Toxoplasmosis- PubMed,  PubMed,  PubMed
Vaccinia- PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed
Werner Syndrome- PubMed,  PubMed
Xeroderma Pigmentosum- PubMed
Yellow Fever- PubMed,  PubMed,  PubMed,  PubMed

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISM (UNIPROT ACCESSION NO.)LINK TO PUBMEDSOURCE
209874Vianna, A.L.Interaction of calcium and magnesium in activating and inhibiting the nucleoside triphosphatase of sarcoplasmic reticulum vesiclesBiochim. Biophys. Acta410389-4061975Oryctolagus cuniculus-
209876Schröder, H.C.; Rottmann, M.; Bachmann, M.; Muller, W.E.G.Purification and characterization of the major nucleoside triphosphatase from rat liver nuclear envelopesJ. Biol. Chem.261663-6681986Rattus norvegicus PubMed
209878Clawson, G.A.; Woo, C.H.; Button, J.; Smuckler, E.A.Photoaffinity labeling of the major nucleosidetriphosphatase of rat liver nuclear envelopeBiochemistry233501-35071984Rattus norvegicus PubMed
209880Paoletti, E.; Moos, B.Two nucleic acid-dependent nucleoside triphosphate phosphohydrolases from vaccinia virus. Nucleotide substrate and polynucleotide cofactor specificitiesJ. Biol. Chem.2493281-32861974Vaccinia virus PubMed
209881Harper, F.; Lamy, F.; Calvert, R.Some properties of a Ca2+- and (or) Mg2+-requiring nucleoside di- and tri-phosphatase(s) associated with the membranes of rat pancreatic zymogen granulesCan. J. Biochem.56565-5671978Rattus norvegicus PubMed
209882Chen, Y.R.; Roux, S.J.Characterization of nucleoside triphosphatase activity in isolated pea nuclei and its photoreversible regulation by lightPlant Physiol.81609-6131986Pisum sativum PubMed
209883McCarty, D.R.; Selman, B.Partial purification of a nucleoside triphosphatase from the inner membrane of the chloroplast envelope of peaArch. Biochem. Biophys.248523-5311986Pisum sativum PubMed
209884McCarty, D.R.; Selman, B.Properties of a partially purified nucleoside triphosphatase (NTPase) from the chloroplast envelope of peaPlant Physiol.80908-9121986Pisum sativum PubMed
209885Chen, Y.R.; Datta, N.; Roux, S.J.Purification and partial characterization of a calmodulin-stimulated nucleoside triphosphatase from pea nucleiJ. Biol. Chem.26210689-106941987Pisum sativum PubMed
209886Plesner L.; Juul B.; Scriver E.; Aalkjaer C.Characterisation of Ca2+ or Mg2+-dependent nucleoside triphosphatase from rat mesenteric small arteriesBiochim. Biophys. Acta1067191-2001991Rattus norvegicus PubMed
209889Pfister T.; Wimmer E.Characterization of the nucleoside triphosphatase activity of poliovirus protein 2C reveals a mechanism by which guanidine inhibits poliovirus replicationJ. Biol. Chem.2746992-70011999Human enterovirus C PubMed
209890Dahlmann N.; Kirchgesser M.Acid nucleoside triphosphatase: partial purification and characterization of a new enzyme from human serumBiochem. Int.20317-3271990Homo sapiens PubMed
656466Zhang, N.; Chen, H.M.; Koch, V.; Schmitz, H.; Minczuk, M.; Stepien, P.; Fattom, A.I.; Naso, R.B.; Kalicharran, K.; Borowski, P.; Hosmane, R.S.Potent inhibition of NTPase/helicase of the West Nile Virus by ring-expanded ('fat') nucleoside analoguesJ. Med. Chem.464776-47892003West Nile virus PubMed
656494Locatelli, G.A.; Gosselin, G.; Spadari, S.; Maga, G.Hepatitis C virus NS3 NTPase/helicase:different stereoselectivity in nucleoside triphosphate utilisation suggests that NTPase and helicase activities are coupled by a nucleotide-dependent rate limiting stepJ. Mol. Biol.313683-6942001Hepatitis C virus PubMed
656663Pfister, T.; Wimmer, E.Polypeptide p41 of a Norwalk-like virus is a nucleic acid-independent nucleoside triphosphataseJ. Virol.751611-16192001Southampton virus (Q04544), Southampton virus PubMed
656664Borowski, P.; Niebuhr, A.; Mueller, O.; Bretner, M.; Felczak, K.; Kulikowski, T.; Schmitz, H.Purification and characterization of West Nile virus nucleoside triphosphatase (NTPase)/helicase: evidence for dissociation of the NTPase and helicase activities of the enzymeJ. Virol.753220-32292001West Nile virus PubMed
667226Bretner, M.; Baier, A.; Kopanska, K.; Najda, A.; Schoof, A.; Reinholz, M.; Lipniacki, A.; Piasek, A.; Kulikowski, T.; Borowski, P.Synthesis and biological activity of 1H-benzotriazole and 1H-benzimidazole analogues - inhibitors of the NTPase/helicase of HCV and of some related FlaviviridaeAntiviral Chem. Chemother.16315-3262005Dengue virus, Hepatitis C virus, Japanese encephalitis virus, West Nile virus PubMed
667694Tanaka, N.; Schwer, B.Characterization of the NTPase, RNA-binding, and RNA helicase activities of the DEAH-box splicing factor Prp22Biochemistry449795-98032005Saccharomyces cerevisiae PubMed
667903Horikawa, M.; Kirkman, N.J.; Mayo, K.E.; Mulders, S.M.; Zhou, J.; Bondy, C.A.; Hsu, S.Y.; King, G.J.; Adashi, E.Y.The mouse germ-cell-specific leucine-rich repeat protein NALP14: a member of the NACHT nucleoside triphosphatase familyBiol. Reprod.72879-8892005Mus musculus PubMed
668616Rakitina, D.V.; Kantidze, O.L.; Leshchiner, A.D.; Solovyev, A.G.; Novikov, V.K.; Morozov, S.Y.; Kalinina, N.O.Coat proteins of two filamentous plant viruses display NTPase activity in vitroFEBS Lett.5794955-49602005no activity in tobacco mosaic virus, potato virus A, potato virus X PubMed
668745Aravind, A.L.; Lakshminarayan, I.L.; Leipe, D.D.; Koonin, E.V.A novel family of P-loop NTPases with an unusual phyletic distribution and transmembrane segments inserted within the NTPase domainGenome Biol.5R302004Agrobacterium tumefaciens, Anabaena sp., Caenorhabditis elegans, Clostridium perfringens, Corynebacterium efficiens, Corynebacterium glutamicum, Cupriavidus metallidurans, Deinococcus radiodurans, Drosophila melanogaster, Escherichia coli, Geobacter sulfurreducens, Homo sapiens, Klebsiella pneumoniae, Leuconostoc mesenteroides, Magnetococcus sp., Magnetococcus sp. mc-1, Nostoc punctiforme, Pseudomonas putida, Rattus norvegicus, Saccharophagus degradans, Staphylococcus epidermidis, Synechocystis sp., Thermotoga maritima, Vibrio parahaemolyticus, Vibrio vulnificus PubMed
669092Xu, W.; Jones, C.R.; Dunn, C.A.; Bessman, M.J.Gene ytkD of Bacillus subtilis encodes an atypical nucleoside triphosphatase member of the Nudix hydrolase superfamilyJ. Bacteriol.1868380-83842004Bacillus subtilis, Bacillus subtilis 168 PubMed
669274Kim, J.; Parker, J.S.; Murray, K.E.; Nibert, M.L.Nucleoside and RNA triphosphatase activities of Orthoreovirus transcriptase cofactor mu2J. Biol. Chem.2794394-44032004mammalian orthoreovirus, mammalian orthoreovirus mORV PubMed
670074Ivanov, K.A.; Ziebuhr, J.Human coronavirus 229E nonstructural protein 13: characterization of duplex-unwinding, nucleoside triphosphatase, and RNA 5-triphosphatase activitiesJ. Virol.787833-78382004Human coronavirus 229E, Human coronavirus 229E (P0C6X1) PubMed
670994Benarroch, D.; Selisko, B.; Locatelli Giada, A.; Maga, G.; Romette, J.L.; Canard, B.The RNA helicase, nucleotide 5'-triphosphatase, and RNA 5'-triphosphatase activities of Dengue virus protein NS3 are Mg2+-dependent and require a functional Walker B motif in the helicase catalytic coreVirology328208-2182004Dengue virus PubMed
671005Li, J.X.; Pang, Y.Z.; Tang, C.S.; Li, Z.Q.Protective effect of taurine on hypochlorous acid toxicity to nuclear nucleoside triphosphatase in isolated nuclei from rat liverWorld J. Gastroenterol.10694-6982004Rattus norvegicus PubMed
684774Wen, G.; Chen, C.; Luo, X.; Wang, Y.; Zhang, C.; Pan, Z.Identification and characterization of the NTPase activity of classical swine fever virus (CSFV) nonstructural protein 3 (NS3) expressed in bacteriaArch. Virol.1521565-15732007Classical swine fever virus, Classical swine fever virus CSFV PubMed
688347Placzek, W.J.; Almeida, M.S.; Wuethrich, K.NMR structure and functional characterization of a human cancer-related nucleoside triphosphataseJ. Mol. Biol.367788-8012007Homo sapiens PubMed
688871Chiang, P.; Sampaleanu, L.M.; Ayers, M.; Pahuta, M.; Howell, P.L.; Burrows, L.L.Functional role of conserved residues in the characteristic secretion NTPase motifs of the Pseudomonas aeruginosa type IV pilus motor proteins PilB, PilT and PilUMicrobiology154114-1262008Pseudomonas aeruginosa PubMed
690616He, Z.G.; Feng, Y.; Wang, J.; Jiang, P.X.The regulatory function of N-terminal AAA+ ATPase domain of eukaryote-like archaeal Orc1/Cdc6 protein during DNA replication initiationArch. Biochem. Biophys.471176-1832008Sulfolobus solfataricus PubMed
690865Hobel, C.F.; Albers, S.V.; Driessen, A.J.; Lupas, A.N.The Sulfolobus solfataricus AAA protein Sso0909, a homologue of the eukaryotic ESCRT Vps4 ATPaseBiochem. Soc. Trans.3694-982008Sulfolobus solfataricus, Sulfolobus solfataricus (Q97ZJ7) PubMed
694432Lewis, R.; Duerr, H.; Hopfner, K.P.; Michaelis, J.Conformational changes of a Swi2/Snf2 ATPase during its mechano-chemical cycleNucleic Acids Res.361881-18902008Sulfolobus solfataricus PubMed
695127Sheng, D.; Li, M.; Jiao, J.; Ni, J.; Shen, Y.Co-expression with RadA and the characterization of stRad55B, a RadA paralog from the hyperthermophilic crenarchaea Sulfolobus tokodaiiSci. China C Life Sci.5160-652008Sulfolobus tokodaii PubMed
696153Sivuk, V.F.; Rusina, I.M.; Makarchikov, A.F.Purification and characteristics of functional properties of soluble nucleoside triphosphatase (apyrase) from bovine brainBiochemistry (Moscow)731047-10522008Bos taurus PubMed
696275Buchko, G.W.; Litvinova, O.; Robinson, H.; Yakunin, A.F.; Kennedy, M.A.Functional and structural characterization of DR_0079 from Deinococcus radiodurans, a novel nudix hydrolase with a preference for cytosine (deoxy)ribonucleoside 5-di- and triphosphatesBiochemistry476571-65822008Deinococcus radiodurans, Deinococcus radiodurans (Q9RXX6) PubMed
718494Matoba, K.; Shiba, T.; Takeuchi, T.; Sibley, L.D.; Seiki, M.; Kikyo, F.; Horiuchi, T.; Asai, T.; Harada, S.Crystallization and preliminary X-ray structural analysis of nucleoside triphosphate hydrolases from Neospora caninum and Toxoplasma gondiiActa Crystallogr. Sect. F661445-14482010Neospora caninum (C9K7J7), Toxoplasma gondii (Q27893) PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 3.6.1.15)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)