Information on EC 3.4.22.50 - V-cath endopeptidase:
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EC NUMBERCOMMENTARY
3.4.22.50-

RECOMMENDED NAMEGeneOntology No.
V-cath endopeptidase-

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
endopeptidase of broad specificity, hydrolyzing substrates of both cathepsin L and cathepsin B
show the reaction diagram		----

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
hydrolysis of peptide bond----

PATHWAYKEGG LinkMetaCyc Link
No entries in this field

SYSTEMATIC NAMEIUBMB Comments
No entries in this field

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
AcNPV protease----
baculovirus cathepsin----
BmNPV protease----
cathepsinCydia pomonella granulovirus--699889
cysteine proteaseBombyx mori NPV--681700
NPV protease----
nucleopolyhedrosis virus protease----
V-cathBombyx mori NPV--681700
V-cathCydia pomonella granulovirus--699889
viral cathepsin----
viral cysteine proteaseCydia pomonella granulovirus--699889

CAS REGISTRY NUMBERCOMMENTARY
316365-69-2-
9001-92-7-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Autographa californica nucleopolyhedrovirus-638872P25783SwissProtManually annotated by BRENDA team
Autographa californica nucleopolyhedrovirusbaculovirus638871P25783SwissProtManually annotated by BRENDA team
Autographa californica nucleopolyhedrovirusin conjunction with chitinase, the enzyme promotes liquefaction of the host in the latter stages of infection638874P25783SwissProtManually annotated by BRENDA team
Bombyx mori NPV-681700--Manually annotated by BRENDA team
Bombyx mori NPVbaculovirus, enzyme may aid in the degradation of host proteins as a source of amino acids for viral protein production638870P41721SwissProtManually annotated by BRENDA team
Choristoneura fumiferana MNPVsruce budworm virus638873--Manually annotated by BRENDA team
Cydia pomonella granulovirusCpGV-M1699889--Manually annotated by BRENDA team
Cydia pomonella granulovirus CpGV-M1CpGV-M1699889--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
azocasein + H2O?
show the reaction diagram		Autographa californica nucleopolyhedrovirusP25783-638871--?
Azocoll + H2O?
show the reaction diagram		Bombyx mori NPVP41721-638870--?
Azocoll + H2O?
show the reaction diagram		Cydia pomonella granulovirus--699889--?
N-benzyloxycarbonyl-Arg-Arg-7-amino-4-methylcoumarin + H2O?
show the reaction diagram		Autographa californica nucleopolyhedrovirusP25783-638872--?
N-benzyloxycarbonyl-Leu-Arg-7-amino-4-methylcoumarin + H2O?
show the reaction diagram		Autographa californica nucleopolyhedrovirusP25783-638872--?
N-benzyloxycarbonyl-Phe-Arg-7-amino-4-methylcoumarin + H2O?
show the reaction diagram		Autographa californica nucleopolyhedrovirusP25783-638872--?
N-benzyloxycarbonyl-Val-Arg-7-amino-4-methylcoumarin + H2O?
show the reaction diagram		Autographa californica nucleopolyhedrovirusP25783-638872--?
N-benzyloxycarbonyl-Val-Val-Arg-7-amino-4-methylcoumarin + H2O?
show the reaction diagram		Autographa californica nucleopolyhedrovirusP25783-638872--?

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
No entries in this field

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
No entries in this field

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
No entries in this field

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
antipainAutographa californica nucleopolyhedrovirusP257830.00005 mM, complete inhibition638872 2D-image
Chicken cystatinAutographa californica nucleopolyhedrovirusP257830.0001 mM, complete inhibition638872-
E64Bombyx mori NPVP41721cysteine protease specific inhibitor638870 2D-image
E64Autographa californica nucleopolyhedrovirusP25783strong inhibition638871 2D-image
E64Autographa californica nucleopolyhedrovirusP257830.0001 mM, complete inhibition638872 2D-image
iodoacetateAutographa californica nucleopolyhedrovirusP257830.05 mM, 80% inhibition638872 2D-image
LeupeptinAutographa californica nucleopolyhedrovirusP25783strong inhibition638871 2D-image
LeupeptinAutographa californica nucleopolyhedrovirusP257830.00005 mM, complete inhibition638872 2D-image
trans-epoxysuccinyl-L-leucylamido-(4-guanidino)butaneCydia pomonella granulovirus-10 microM699889 2D-image

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
UreaAutographa californica nucleopolyhedrovirusP257833 M, enhances activity638871 2D-image

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.003-Z-Arg-Arg-7-amido-4-methylcoumarinAutographa californica nucleopolyhedrovirusP25783-638872 2D-image
0.022-Z-Leu-Arg-7-amino-4-methylcoumarinAutographa californica nucleopolyhedrovirusP25783-638872 2D-image
0.039-Z-Phe-Arg-7-amino-4-methylcoumarinAutographa californica nucleopolyhedrovirusP25783-638872 2D-image
0.043-Z-Val-Arg-7-amino-4-methylcoumarinAutographa californica nucleopolyhedrovirusP25783-638872 2D-image
0.058-Z-Val-Val-Arg-7-amino-4-methylcoumarinAutographa californica nucleopolyhedrovirusP25783-638872 2D-image

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.73-Z-Arg-Arg-7-amido-4-methylcoumarinAutographa californica nucleopolyhedrovirusP25783-638872 2D-image
0.73-Z-Leu-Arg-7-amino-4-methylcoumarinAutographa californica nucleopolyhedrovirusP25783-638872 2D-image
0.95-Z-Phe-Arg-7-amino-4-methylcoumarinAutographa californica nucleopolyhedrovirusP25783-638872 2D-image
0.94-Z-Val-Arg-7-amino-4-methylcoumarinAutographa californica nucleopolyhedrovirusP25783-638872 2D-image
0.51-Z-Val-Val-Arg-7-amino-4-methylcoumarinAutographa californica nucleopolyhedrovirusP25783-638872 2D-image

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
55.5Autographa californica nucleopolyhedrovirusP25783bell-shaped profile, hydrolysis of Z-Phe-Arg-7-amino-4-methylcoumarin and Z-Arg-Arg-7-amino-4-methylcoumarin638872
5-Autographa californica nucleopolyhedrovirusP25783-638871

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
37.5Autographa californica nucleopolyhedrovirusP25783hydrolysis of Z-Phe-Arg-7-amino-4-methylcoumarin638872
47.5Autographa californica nucleopolyhedrovirusP25783hydrolysis of Z-Arg-Arg-7-amino-4-methylcoumarin638872

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
No entries in this field

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
No entries in this field

PDBSCOPCATHORGANISM
No entries in this field

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
?Autographa californica nucleopolyhedrovirusP25783x * 27500, 35500 Da and 32000 Da species are probaly precursors of the mature enzyme, immunoblot; x * 37000, deduced from nucleotide sequence638871

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
glycoproteinAutographa californica nucleopolyhedrovirusP25783N-glycosylation may occur at Asn158638871

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
No entries in this field

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-Autographa californica nucleopolyhedrovirusP25783638872

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
expression in Sf9 insect cellsAutographa californica nucleopolyhedrovirusP25783638872
sequencing of BmNPVBombyx mori NPVP41721638870
expression in Escherichia coliCydia pomonella granulovirus-699889

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
additional informationBombyx mori NPV-in this study a cysteine protease-deleted Bombyx mori multiple nucleopolyhedrovirus (CPD-BmMNPV) bacmid using the lambda recombination system is constructed. The protease activities of Bombyx mori cells and silkworm larvae infected with this CPD-BmMNPV bacmid are reduced by 94% and 85%, respectively. By using this system, a GFP(uv)-beta1,3-N-acetylglucosaminyltransferase 2 (GFP(uv)-beta3GnT2) fusion protein is successfully expressed in silkworm larvae with less protein degradation and without larvae liquefaction681700
additional informationCydia pomonella granulovirus-v-cath-deletion mutant, chloramphenicol acetyl transferase gene replaced the cathepsin gene699889

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
biotechnologyBombyx mori NPV-this CPD-BmMNPV bacmid system provides rapid protein production in silkworms and can be used for the production of recombinant eukaryotic proteins without proteolytic degradation681700

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISMLINK TO PUBMEDSOURCE
638870Ohkawa, T.; Majima, K.; Maeda, S.A cysteine protease encoded by the baculovirus Bombyx mori nuclear polyhedrosis virusJ. Virol.686619-66251994Bombyx mori NPV PubMed
638871Slack, J.M.; Kuzio, J.; Faulkner, P.Characterization of v-cath, a cathepsin L-like proteinase expressed by the baculovirus Autographa californica multiple nuclear polyhedrosis virusJ. Gen. Virol.761091-10981995Autographa californica nucleopolyhedrovirus-
638872Bromme, D.; Okamoto, K.The baculovirus cysteine protease has a cathepsin B-like S2-subsite specificityBiol. Chem. Hoppe-Seyler376611-6151995Autographa californica nucleopolyhedrovirus PubMed
638873Hill, J.E.; Kuzio, J.; Faulkner, P.Identification and characterization of the v-cath gene of the baculovirus, CfMNPVBiochim. Biophys. Acta1264275-2781995Choristoneura fumiferana MNPV PubMed
638874Hawtin, R.E.; Zarkowska, T.; Arnold, K.; Thomas, C.J.; Gooday, G.W.; King, L.A.; Kuzio, J.A.; Possee, R.D.Liquefaction of Autographa californica nucleopolyhedrovirus-infected insects is dependent on the integrity of virus-encoded chitinase and cathepsin genesVirology238243-2531997Autographa californica nucleopolyhedrovirus PubMed
681700Hiyoshi, M.; Kageshima, A.; Kato, T.; Park, E.Y.Construction of a cysteine protease deficient Bombyx mori multiple nucleopolyhedrovirus bacmid and its application to improve expression of a fusion proteinJ. Virol. Methods14491-972007Bombyx mori NPV PubMed
699889Hilton, S.; Kemp, E.; Keane, G.; Winstanley, D.A bacmid approach to the genetic manipulation of granulovirusesJ. Virol. Methods15256-622008Cydia pomonella granulovirus PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 3.4.22.50)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)