Information on EC 3.4.13.5 - Xaa-methyl-His dipeptidase:
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The lowest common taxonomy group for this enzyme is: Euteleostei

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EC NUMBERCOMMENTARY
3.4.13.5-

RECOMMENDED NAMEGeneOntology No.
Xaa-methyl-His dipeptidase-

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
Hydrolysis of anserine (beta-alanyl-/-Npi-methyl-L-histidine), carnosine, homocarnosine, glycyl-/-leucine and other dipeptides with broad specificity.
show the reaction diagram		----

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
hydrolysis of peptide bond--dipeptide hydrolase-

PATHWAYKEGG LinkMetaCyc Link
No entries in this field

SYSTEMATIC NAMEIUBMB Comments
No entries in this field

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
acetylhistidine deacetylase----
aminoacyl-methylhistidine dipeptidase----
aminoacyl-pros-methyl-L-histidine hydrolaseKatsuwonus pelamis--665092
anserinase----
anserinaseOreochromis niloticusQ3LHN4-664899
deacetylase, acetylhistidine----
dipeptidase, aminoacylmethylhistidine----
EC 3.4.3.4--formerly-
EC 3.5.1.34--formerly-
NacHDE----
Nalpha-acetylhistidine deacetylase----
X-methyl-His dipeptidase----
X-methyl-His dipeptidaseKatsuwonus pelamis--665092
Xaa-methyl-His dipeptidase----
Xaa-methyl-His dipeptidaseOreochromis niloticusQ3LHN4-664899

CAS REGISTRY NUMBERCOMMENTARY
37289-11-5-
9027-38-7-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Gadus callarias-36079--Manually annotated by BRENDA team
Katsuwonus pelamis-35025, 36080, 665092--Manually annotated by BRENDA team
Oncorhynchus mykiss-36081--Manually annotated by BRENDA team
Oreochromis niloticus-35019--Manually annotated by BRENDA team
Oreochromis niloticusanserinase precursor664899Q3LHN4SwissProtManually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
Ala-His + H2OAla + His
show the reaction diagram		Oreochromis niloticusQ3LHN4-664899--?
Ala-Pro + H2OAla + Pro
show the reaction diagram		Oreochromis niloticusQ3LHN4-664899--?
anserine + H2Obeta-Ala + 1-methylhistidine
show the reaction diagram		Gadus callarias--36079-36079?
anserine + H2Obeta-Ala + 1-methylhistidine
show the reaction diagram		Oncorhynchus mykiss-16% of the activity with N-acetyl-L-His, brain enzyme36081--?
anserine + H2Obeta-Ala + 1-methylhistidine
show the reaction diagram		Katsuwonus pelamis-9% of the activity with carnosine35025--?
anserine + H2Obeta-alanine + 3-methyl-L-histidine
show the reaction diagram		Oreochromis niloticusQ3LHN4-664899--?
carnosine + H2O?
show the reaction diagram		Katsuwonus pelamis--35025--?
carnosine + H2O?
show the reaction diagram		Oncorhynchus mykiss-83% of the activity with N-acetyl-L-His, brain enzyme36081--?
carnosine + H2Obeta-alanine + L-His
show the reaction diagram		Oreochromis niloticusQ3LHN4-664899--?
Gly-Gly + H2OGly + Gly
show the reaction diagram		Oncorhynchus mykiss-385% of the activity with N-acetyl-L-His36081--?
Gly-Gly + H2OGly
show the reaction diagram		Oreochromis niloticusQ3LHN4-664899--?
Gly-His + H2OGly + His
show the reaction diagram		Katsuwonus pelamis--665092--?
Gly-His + H2OGly + His
show the reaction diagram		Katsuwonus pelamis-68% of the activity with carnosine35025--?
Gly-L-His + H2OGly + L-His
show the reaction diagram		Oncorhynchus mykiss-110% of the activity with N-acetyl-L-His, brain enzyme36081--?
Gly-L-Leu + H2OGly + L-Leu
show the reaction diagram		Oncorhynchus mykiss-832% of the activity with N-acetyl-L-His, brain enzyme36081--?
Gly-L-Leu + H2OGly + L-Leu
show the reaction diagram		Katsuwonus pelamis-42% of the activity with carnosine35025---
Gly-Leu + H2OGly + Leu
show the reaction diagram		Katsuwonus pelamis--665092--?
His-Gly + H2OHis + Gly
show the reaction diagram		Oreochromis niloticusQ3LHN4-664899--?
homocarnosine + H2O?
show the reaction diagram		Oncorhynchus mykiss-67% of the activity with N-acetyl-L-His, brain enzyme36081--?
homocarnosine + H2O?
show the reaction diagram		Katsuwonus pelamis-102% of the activity with carnosine35025--?
homocarnosine + H2O4-aminobutyric acid + L-His
show the reaction diagram		Oreochromis niloticusQ3LHN4-664899--?
L-Ala-L-His + H2OL-Ala + L-His
show the reaction diagram		Oncorhynchus mykiss-105% of the activity with N-acetyl-L-His, brain enzyme36081--?
L-Leu-Gly + H2OL-Leu + Gly
show the reaction diagram		Oncorhynchus mykiss-101% of the activity with N-acetyl-L-His, brain enzyme36081--?
N-acetyl-L-Glu + H2OL-Glu + acetate
show the reaction diagram		Oncorhynchus mykiss-23% of the activity with N-acetyl-L-His, brain enzyme36081--?
N-acetyl-L-His + H2OHis + acetate
show the reaction diagram		Oncorhynchus mykiss--36081-36081?
N-acetyl-L-His + H2OHis + acetate
show the reaction diagram		Katsuwonus pelamis--36080--?
N-acetyl-L-His + H2OHis + acetate
show the reaction diagram		Katsuwonus pelamis-62% of the activity with carnosine35025--?
N-acetyl-L-Leu + H2OL-Leu + acetate
show the reaction diagram		Oncorhynchus mykiss-110% of the activity with N-acetyl-L-His, brain enzyme36081--?
N-acetyl-L-methionine + H2OL-Met + acetate
show the reaction diagram		Katsuwonus pelamis--36080--?
N-acetyl-L-methionine + H2OL-Met + acetate
show the reaction diagram		Oncorhynchus mykiss-254% of the activity with N-acetyl-L-His, brain enzyme36081--?
N-acetyl-L-methionine + H2OL-Met + acetate
show the reaction diagram		Katsuwonus pelamis-12% of the activity with carnosine35025--?
N-acetyl-L-Trp + H2OL-Trp + acetate
show the reaction diagram		Oncorhynchus mykiss-30% of the activity with N-acetyl-L-His, brain enzyme36081--?
Nalpha-acetyl-L-His + H2OHis + acetate
show the reaction diagram		Oreochromis niloticusQ3LHN4-664899--?
Nalpha-chloro-acetyl-L-Leu + H2Ochloroacetate + Leu
show the reaction diagram		Oreochromis niloticusQ3LHN4-664899--?
Nalpha-chloroacetyl-L-Leu + H2OL-Leu + chloroacetate
show the reaction diagram		Oncorhynchus mykiss-1235% of the activity with N-acetyl-L-His, brain enzyme36081--?
Pro-Gly + H2OPro + Gly
show the reaction diagram		Oreochromis niloticusQ3LHN4-664899--?
Leu-Gly + H2OLeu + Gly
show the reaction diagram		Oreochromis niloticusQ3LHN4-664899--?
additional information?-Katsuwonus pelamis-no activity with: N-acetylhistamine, N-acetyl-L-Trp, N-acetyl-L-Tyr, N-acetyl-L-Ala, N-acetyl-L-Val, N-acetylglycine, N-acetyl-L-Glu, N-acetyl-L-Asp, N-acetyl-L-Cys, N-acetyl-L-Orn, enzyme may be required to degrade N-acetyl-L-histidine after formation of a transient capsular complex36080---

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
additional information?-Katsuwonus pelamis-enzyme may be required to degrade N-acetyl-L-histidine after formation of a transient capsular complex36080--

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
No entries in this field

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
Co2+Katsuwonus pelamis-activates35025
Zn2+Katsuwonus pelamis-activates35025
Zn2+Gadus callarias-activates36079
Co2+Gadus callarias-0.1 mM, 20% activation36079
additional informationOreochromis niloticusQ3LHN4sequence analysis shows that the enzyme is a member of the M20A metallopeptidase subfamily664899

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
1,10-phenanthrolineOncorhynchus mykiss--36081 2D-image
2-mercaptoethanolKatsuwonus pelamis--35025 2D-image
Ag+Gadus callarias-1 mM, 90% inhibition36079 2D-image
ATPOncorhynchus mykiss--36081 2D-image
bestatinOncorhynchus mykiss-0.01 mM, 15% inhibition of the enzyme from eye, 1% inhibition of the enzyme from brain36081 2D-image
ChloropromazineKatsuwonus pelamis--35025 2D-image
Co2+Gadus callarias-1 mM, 10% inhibition36079 2D-image
CoAOncorhynchus mykiss--36081 2D-image
Cu2+Oncorhynchus mykiss--36081 2D-image
cyanideGadus callarias--36079 2D-image
CysGadus callarias-90% inhibition at 1 mM, 40% inhibition at 0.1 mM36079 2D-image
dithiothreitolKatsuwonus pelamis--35025 2D-image
EDTAKatsuwonus pelamis-restored by Zn2+ and Co2+35025 2D-image
EDTAOncorhynchus mykiss--36081 2D-image
Fe3+Gadus callarias-1 mM, 94% inhibition36079 2D-image
Hg2+Katsuwonus pelamis-HgCl235025 2D-image
Hg2+Oncorhynchus mykiss--36081 2D-image
Mn2+Gadus callarias-1 mM, 30% inhibition36079 2D-image
Pb2+Gadus callarias-1 mM, 80% inhibition36079 2D-image
Pb2+Oncorhynchus mykiss--36081 2D-image
PCMBKatsuwonus pelamis--35025 2D-image
PCMBOncorhynchus mykiss--36081 2D-image
phenylmethylsulfonyl fluorideKatsuwonus pelamis--35025 2D-image
phosphateKatsuwonus pelamis--35025 2D-image
TrifluorperazineKatsuwonus pelamis--35025 2D-image

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
additional information-additional informationGadus callarias--36079-

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
0.13-Katsuwonus pelamis--36080
0.175-Katsuwonus pelamis--35025
1.03-Oncorhynchus mykiss-brain36081
1.27-Oncorhynchus mykiss-eye36081
9.27-Oreochromis niloticusQ3LHN4-664899

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
66.5Oncorhynchus mykiss--36081
67Katsuwonus pelamis--35025
77.3Katsuwonus pelamis--36080
7.3-Gadus callarias-anserine36079

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
59Oncorhynchus mykiss-less than 10% of maximal activity at pH 5.0 and 9.036081
5.28.5Katsuwonus pelamis-50% of maximal activity at pH 5.2 and at pH 8.535025
68Gadus callarias-pH 6.0: about 35% of maximal activity, pH 8.0: about 55% of maximal activity36079
6.57.8Katsuwonus pelamis-activity is sharply reduced below pH 6.5 and above pH 7.836080

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
50-Oncorhynchus mykiss--36081

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
1060Oncorhynchus mykiss-10°C: about 50% of maximal activity, brain and eye enzyme, 60°C, about 40% of maximal activity, brain enzyme, 60°C: about 90% of maximal activity, eye enzyme36081

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
5.3-Oreochromis niloticusQ3LHN4calculated from sequence664899

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
brainKatsuwonus pelamis-very rich source35025Manually annotated by BRENDA team
brainOncorhynchus mykiss-all regions of the brain except for the spinal cord and the optic nerve36081Manually annotated by BRENDA team
brainOreochromis niloticusQ3LHN4-664899Manually annotated by BRENDA team
brainKatsuwonus pelamis--665092Manually annotated by BRENDA team
eyeOncorhynchus mykiss--36081Manually annotated by BRENDA team
gall bladderKatsuwonus pelamis-trace amounts35025Manually annotated by BRENDA team
intestineKatsuwonus pelamis-moderate activity35025Manually annotated by BRENDA team
kidneyOreochromis niloticusQ3LHN4-664899Manually annotated by BRENDA team
lensKatsuwonus pelamis-cortex, concentrated in the capsule at levels up to 3 mg/ml36080Manually annotated by BRENDA team
liverKatsuwonus pelamis-moderate activity35025Manually annotated by BRENDA team
muscleOreochromis niloticus--35019Manually annotated by BRENDA team
muscleGadus callarias-skeletal36079Manually annotated by BRENDA team
ocular fluidKatsuwonus pelamis--665092Manually annotated by BRENDA team
ovaryKatsuwonus pelamis-trace amounts35025Manually annotated by BRENDA team
retinaOreochromis niloticusQ3LHN4-664899Manually annotated by BRENDA team
skeletal muscleOreochromis niloticusQ3LHN4-664899Manually annotated by BRENDA team
spleenKatsuwonus pelamis-trace amounts35025Manually annotated by BRENDA team
stomachKatsuwonus pelamis-trace amounts35025Manually annotated by BRENDA team
vitreous humorKatsuwonus pelamis-very rich source35025Manually annotated by BRENDA team
white muscleKatsuwonus pelamis-trace amounts35025Manually annotated by BRENDA team

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
No entries in this field

PDBSCOPCATHORGANISM
No entries in this field

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
83000-Katsuwonus pelamis-gel filtration36080
120000-Oncorhynchus mykiss-brain and eye enzyme, gel filtration36081
120000-Oreochromis niloticusQ3LHN4gel filtration664899
120000-Katsuwonus pelamis-gel filtration665092

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
?Oreochromis niloticusQ3LHN4x * 53311, calculated from sequence664899
dimerOncorhynchus mykiss-2 * 55000, SDS-PAGE36081

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
4-Katsuwonus pelamis-unstable at35025
4.76.4Katsuwonus pelamis-maximal stability at 30°C, pH 4.7-6.4, in presence of ZnSO435025
5.28.1Oncorhynchus mykiss-maximal stability36081
8.5-Katsuwonus pelamis-unstable at35025

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
30-Katsuwonus pelamis-maximal stability at 30°C, pH 4.7-6.4, in presence of ZnSO435025

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
partialGadus callarias-36079
-Katsuwonus pelamis-35025, 36080
partialKatsuwonus pelamis-665092
-Oncorhynchus mykiss-36081
-Oreochromis niloticusQ3LHN4664899

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-Oreochromis niloticusQ3LHN4664899

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISMLINK TO PUBMEDSOURCE
35019Yamada, S.; Tanaka, Y.; Sameshima, M.; Furuichi, M.Occurence of Nalpha-acetylhistidine in the muscle and deacetylation by several tissues of nile tilapia (Oreochromis niloticus)Comp. Biochem. Physiol. B103579-5831992Oreochromis niloticus-
35025Lenney, J.F.; Baslow, M.H.; Sugiyama, G.H.Similarity of tuna N-acetylhistidine deacetylase and cod fish anserinaseComp. Biochem. Physiol. B61253-2581978Katsuwonus pelamis PubMed
36079Jones, N.R.The free amino acids of fish. 1-Methylhistidine and beta-alanine liberation by skeletal muscle anserinase of codling (Gadus callarias)Biochem. J.6081-871955Gadus callarias PubMed
36080Baslow, M.H.; Lenney, J.F.Alpha-N-acetyl-L-histidine amidohydrolase activity from the brain of the skipjack tuna Katsuwonus pelamisCan. J. Biochem.45337-3491967Katsuwonus pelamis PubMed
36081Yamada, S.; Tanaka, Y.; Sameshima, M.; Furuichi, M.Properties of Nalpha-acetylhistidine deacetylase in brain of rainbow trout Oncorhynchus mykissComp. Biochem. Physiol. B Comp. Biochem.106309-3151993Oncorhynchus mykiss-
664899Yamada, S.; Tanaka, Y.; Ando, S.Purification and sequence identification of anserinaseFEBS J.2726001-60132005Oreochromis niloticus PubMed
665092McDonald, J.K.X-methyl-His dipeptidaseHandbook of proteolytic enzymes (Barrett, A.J., Rawlings, N.D., Woessner, J.F., eds.) Academic Press22109-21102004Katsuwonus pelamis-

LINKS TO OTHER DATABASES (specific for EC-Number 3.4.13.5)
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KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)