Information on EC 3.2.2.7 -

Information on EC 3.2.2.7 - adenosine nucleosidase:

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EC NUMBER	COMMENTARY
3.2.2.7	-

RECOMMENDED NAME	GeneOntology No.
adenosine nucleosidase	GO:0047622

REACTION	REACTION DIAGRAM	COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
adenosine + H2O = D-ribose + adenine		also acts on adenosine N-oxide	Camellia sinensis	-	137109
adenosine + H2O = D-ribose + adenine		also acts on adenosine N-oxide	Hordeum vulgare	-	137111
adenosine + H2O = D-ribose + adenine		-	-	-	-

REACTION TYPE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
hydrolysis of N-glycosyl bond	-	-	-	-

PATHWAY	KEGG Link	MetaCyc Link
adenine and adenosine salvage II	-	PWY-6605

SYSTEMATIC NAME	IUBMB Comments
adenosine ribohydrolase	Also acts on adenosine N-oxide.

SYNONYMS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
adenosinase	-	-	-	-
adenosine hydrolase	-	-	-	-
ANase	-	-	-	-
N-ribosyladenine ribohydrolase	-	-	-	-

CAS REGISTRY NUMBER	COMMENTARY
9075-41-6	-

ORGANISM	COMMENTARY	LITERATURE	SEQUENCE CODE	SEQUENCE DB	SOURCE
Acacia dealbata	-	137104	-	-
Arabidopsis thaliana	-	700741, 710272	-	-
Avicennia marina	mangrove shrub, high activity of enzyme	657086	-	-
Brassica oleracea	var. gemmifera	137113	-	-
Bruguiera gymnorhiza	mangrove shrub	657086	-	-
Camellia sinensis	-	655545	-	-
Camellia sinensis	tea	137101, 137109	-	-
Catharanthus roseus	-	681129, 682289	-	-
Coffea arabica	L. cv. Catimor	670512	-	-
Crithidia fasciculata	-	137108	-	-
Fraxinus excelsior	-	137104	-	-
Helianthus tuberosus	artichoke	137051	-	-
Hordeum vulgare	barley	137102, 137106, 137111	-	-
Kandelia candel	mangrove shrub	657086	-	-
Larix decidua	-	137104	-	-
Lumnitzera racemosa	mangrove shrub	657086	-	-
Lupinus luteus	-	137099, 137110	-	-
Medicago sativa	alfalfa	137100	-	-
no activity in Ceratodon purpureus	-	137104	-	-
no activity in Equisetum arvense	-	137104	-	-
no activity in Leishmania braziliensis	-	137108	-	-
no activity in Leishmania donovani	-	137108	-	-
no activity in Leishmania mexicana	-	137108	-	-
no activity in Leishmania tarentolae	-	137108	-	-
no activity in Populus alba	-	137104	-	-
Pemphis acidula	mangrove shrub	657086	-	-
Rhizophora stylosa	mangrove shrub	657086	-	-
Solanum lycopersicum	three forms: R1, R2 and Lf	137103	-	-
Solanum tuberosum	-	137112, 676653	-	-
Sonneratia alba	mangrove shrub	657086	-	-
Tilia cordata	-	137104	-	-
Triticum aestivum	-	137107	-	-
Trypanosoma cruzi	-	137108	-	-
Ulmus carpinifolia	-	137104	-	-

GENERAL INFORMATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

SUBSTRATE	PRODUCT	REACTION DIAGRAM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY/ Substrate	LITERATURE/ Substrate	COMMENTARY/ Product	LITERATURE/ Product	Reversibility r=reversible ir=irreversible ?=not specified
2'-deoxyadenosine + H2O	6-aminopurine + 2-deoxy-D-ribofuranose		Lupinus luteus	-	one third lowered activity compared to adenosine as substrate	137099	-	137099	?
2'-deoxyadenosine + H2O	6-aminopurine + 2-deoxy-D-ribofuranose		Camellia sinensis	-	3-4 times greater hydrolysis compared to adenosine	137109	-	137109	?
2'-deoxyadenosine + H2O	6-aminopurine + 2-deoxy-D-ribofuranose		Hordeum vulgare	-	hydrolyzes at 96% of the rate of adenosine	137102	-	137102	?
2'-deoxyadenosine + H2O	6-aminopurine + 2-deoxy-D-ribofuranose		Solanum tuberosum	-	hydrolysis at the same rate as for adenosine	137112	-	137112	?
2'-deoxyadenosine + H2O	6-aminopurine + 2-deoxy-D-ribofuranose		Brassica oleracea	-	hydrolysis at the same rate as for adenosine	137113	-	137113	?
2'-deoxyadenosine + H2O	6-aminopurine + 2-deoxy-D-ribofuranose		Lupinus luteus	-	6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside	137099	-	137099	?
2'-deoxyadenosine + H2O	6-aminopurine + 2-deoxy-D-ribofuranose		Hordeum vulgare	-	6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside	137102	-	137102	?
2'-deoxyadenosine + H2O	6-aminopurine + 2-deoxy-D-ribofuranose		Hordeum vulgare	-	6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside	137111	-	137111	?
2'-deoxyadenosine + H2O	6-aminopurine + 2-deoxy-D-ribofuranose		Solanum tuberosum	-	6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside	137112	-	137112	?
2'-deoxyadenosine + H2O	6-aminopurine + 2-deoxy-D-ribofuranose		Camellia sinensis	-	6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside	137109	-	137109	?
2'-deoxyadenosine + H2O	6-aminopurine + 2-deoxy-D-ribofuranose		Brassica oleracea	-	6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside	137113	-	137113	?
3'-deoxyadenosine + H2O	6-aminopurine + 3-deoxy-D-ribofuranose		Lupinus luteus	-	6-aminopurine-9-3'-deoxy-beta-D-ribofuranoside, cordycepin, very low activity	137099	-	137099	?
5'-deoxyadenosine + H2O	6-aminopurine + 5-deoxy-D-ribofuranose		Lupinus luteus	-	6-aminopurine-9-5'-deoxy-beta-D-ribofuranoside, best substrate	137099	-	137099	?
6-aminopurine-9-beta-D-ribofuranosyl-N1-oxide + H2O	6-aminopurine-N1-oxide + D-ribofuranose		Hordeum vulgare	-	same hydrolysis rate than for adenosine or 2'-deoxyadenosine	137111	-	137111	?
6-aminopurine-9-beta-D-ribofuranosyl-N1-oxide + H2O	6-aminopurine-N1-oxide + D-ribofuranose		Camellia sinensis	-	rapid hydrolysis	137109	-	137109	?
6-benzylaminopurine riboside + H2O	6-benzylaminopurine + D-ribose		Lupinus luteus	-	low activity	137099	-	137099	?
7-methyl-6-aminopurine-9-beta-D-ribofuranoside + H2O	7-methyl-6-aminopurine + D-ribofuranose		Camellia sinensis	-	5fold faster hydrolysis compared to adenosine	137101	-	137101	?
adenosine + H2O	6-aminopurine + D-ribofuranose		Lupinus luteus	-	component of the purine salvage pathway	137099	-	137099	?
adenosine + H2O	6-aminopurine + D-ribofuranose		Camellia sinensis	-	component of the purine salvage pathway	137101	-	137101	?
adenosine + H2O	6-aminopurine + D-ribofuranose		Camellia sinensis	-	component of the purine salvage pathway	137109	-	137109	?
adenosine + H2O	6-aminopurine + D-ribofuranose		Crithidia fasciculata, Trypanosoma cruzi	-	component of the purine salvage pathway	137108	-	137108	?
adenosine + H2O	6-aminopurine + D-ribofuranose		Solanum lycopersicum	-	component of the purine salvage pathway	137103	-	137103	ir
adenosine + H2O	6-aminopurine + D-ribofuranose		Triticum aestivum	-	implicated in metabolism of cytokinins	137107	-	137107	?
adenosine + H2O	6-aminopurine + D-ribofuranose		Solanum lycopersicum	-	implicated in metabolism of cytokinins	137103	-	137103	ir
adenosine + H2O	6-aminopurine + D-ribofuranose		Medicago sativa	-	involved in maintaining an active pool of S-adenosyl-L-methionine for transmethylation reactions	137100	-	137100	?
adenosine + H2O	6-aminopurine + D-ribofuranose		Triticum aestivum	-	6-aminopurine-9-beta-D-ribofuranoside	137107	-	137107	?
adenosine + H2O	6-aminopurine + D-ribofuranose		Lupinus luteus	-	6-aminopurine-9-beta-D-ribofuranoside	137099	-	137099	?
adenosine + H2O	6-aminopurine + D-ribofuranose		Lupinus luteus	-	6-aminopurine-9-beta-D-ribofuranoside	137110	-	137110	?
adenosine + H2O	6-aminopurine + D-ribofuranose		Hordeum vulgare	-	6-aminopurine-9-beta-D-ribofuranoside	137111	-	137111	?
adenosine + H2O	6-aminopurine + D-ribofuranose		Hordeum vulgare	-	6-aminopurine-9-beta-D-ribofuranoside	137102	-	137102	ir
adenosine + H2O	6-aminopurine + D-ribofuranose		Hordeum vulgare	-	6-aminopurine-9-beta-D-ribofuranoside	137106	-	137106	ir
adenosine + H2O	6-aminopurine + D-ribofuranose		Solanum tuberosum	-	6-aminopurine-9-beta-D-ribofuranoside	137112	-	137112	ir
adenosine + H2O	6-aminopurine + D-ribofuranose		Camellia sinensis	-	6-aminopurine-9-beta-D-ribofuranoside	137101	-	137101	?
adenosine + H2O	6-aminopurine + D-ribofuranose		Camellia sinensis	-	6-aminopurine-9-beta-D-ribofuranoside	137109	-	137109	?
adenosine + H2O	6-aminopurine + D-ribofuranose		Crithidia fasciculata	-	6-aminopurine-9-beta-D-ribofuranoside	137108	-	137108	?
adenosine + H2O	6-aminopurine + D-ribofuranose		Medicago sativa	-	6-aminopurine-9-beta-D-ribofuranoside	137100	-	137100	?
adenosine + H2O	6-aminopurine + D-ribofuranose		Trypanosoma cruzi	-	6-aminopurine-9-beta-D-ribofuranoside	137108	-	137108	?
adenosine + H2O	6-aminopurine + D-ribofuranose		Solanum lycopersicum	-	6-aminopurine-9-beta-D-ribofuranoside	137103	-	137103	ir
adenosine + H2O	6-aminopurine + D-ribofuranose		Helianthus tuberosus	-	6-aminopurine-9-beta-D-ribofuranoside	137051	-	137051	ir
adenosine + H2O	6-aminopurine + D-ribofuranose		Brassica oleracea	-	6-aminopurine-9-beta-D-ribofuranoside	137113	-	137113	ir
adenosine + H2O	6-aminopurine + D-ribofuranose		Fraxinus excelsior, Larix decidua, Tilia cordata, Ulmus carpinifolia, Acacia dealbata	-	6-aminopurine-9-beta-D-ribofuranoside	137104	-	137104	ir
adenosine + H2O	D-ribose + adenine		Arabidopsis thaliana	-	-	700741, 710272	-	-	?
adenosine + H2O	D-ribose + adenine		Avicennia marina, Bruguiera gymnorhiza, Rhizophora stylosa, Kandelia candel	-	-	657086	-	-	?
adenosine + H2O	D-ribose + adenine		Coffea arabica	-	-	670512	-	-	?
adenosine + H2O	D-ribose + adenine		Sonneratia alba, Lumnitzera racemosa, Pemphis acidula	-	-	657086	-	-	?
guanosine + H2O	2-amino-1,9-dihydropurine-6-on + D-ribofuranose		Lupinus luteus	-	2-amino-1,9-dihydro-6-oxopurine-9-beta-D-ribofuranoside, two third lowered activity compared to adenosine as substrate	137099	-	137099	?
N6-(DELTA2-isopentenyl)-adenosine + H2O	N6-(DELTA2-isopentenyl)-6-aminopurine + D-ribofuranose		Triticum aestivum	-	N6-(DELTA2-isopentenyl)-6-aminopurine-9-beta-D-ribofuranoside	137107	-	137107	?
purine riboside + H2O	purine + D-ribose		Lupinus luteus	-	low activity	137099	-	137099	?
purine riboside + H2O	purine + D-ribose		Hordeum vulgare	-	low activity	137111	-	137111	?
inosine + H2O	hypoxanthine + D-ribose		Lupinus luteus	-	9-ribosylhydroxanthine, low activity	137099	-	137099	?
additional information	?	-	Pemphis acidula	-	30% of adenosine taken up by leaf disks is hydrolysed to adenine	657086	-	-	-
additional information	?	-	Lumnitzera racemosa	-	30% of adenosine taken up by leaf disks may be converted to salvage products	657086	-	-	-
additional information	?	-	Avicennia marina, Bruguiera gymnorhiza, Rhizophora stylosa, Kandelia candel, Sonneratia alba	-	56-92% of adenosine taken up by leaf disks may be converted to salvage products, efficient adenosine salvage abilitiy in mangrove is coupled to high enzymic activity and is stimulated by salt, proposed metabolic pathway	657086	-	-	-
additional information	?	-	Camellia sinensis	-	proposed pathway for biosynthesis of purine alkaloids with adenosine derived from SAM cycle	655545	-	-	-
additional information	?	-	Arabidopsis thaliana	-	the pure recombinant protein exhibits highest hydrolase activity for uridine, followed by inosine and adenosine	700741	-	-	-

NATURAL SUBSTRATES	NATURAL PRODUCTS	REACTION DIAGRAM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY SUBSTRATE	LITERATURE (Substrate)	COMMENTARY PRODUCT	LITERATURE (Product)
2'-deoxyadenosine + H2O	6-aminopurine + 2-deoxy-D-ribofuranose		Lupinus luteus	-	one third lowered activity compared to adenosine as substrate	137099	-	137099
2'-deoxyadenosine + H2O	6-aminopurine + 2-deoxy-D-ribofuranose		Camellia sinensis	-	3-4 times greater hydrolysis compared to adenosine	137109	-	137109
2'-deoxyadenosine + H2O	6-aminopurine + 2-deoxy-D-ribofuranose		Hordeum vulgare	-	hydrolyzes at 96% of the rate of adenosine	137102	-	137102
2'-deoxyadenosine + H2O	6-aminopurine + 2-deoxy-D-ribofuranose		Solanum tuberosum	-	hydrolysis at the same rate as for adenosine	137112	-	137112
2'-deoxyadenosine + H2O	6-aminopurine + 2-deoxy-D-ribofuranose		Brassica oleracea	-	hydrolysis at the same rate as for adenosine	137113	-	137113
2'-deoxyadenosine + H2O	6-aminopurine + 2-deoxy-D-ribofuranose		Lupinus luteus	-	6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside	137099	-	137099
2'-deoxyadenosine + H2O	6-aminopurine + 2-deoxy-D-ribofuranose		Hordeum vulgare	-	6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside	137102	-	137102
2'-deoxyadenosine + H2O	6-aminopurine + 2-deoxy-D-ribofuranose		Hordeum vulgare	-	6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside	137111	-	137111
2'-deoxyadenosine + H2O	6-aminopurine + 2-deoxy-D-ribofuranose		Solanum tuberosum	-	6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside	137112	-	137112
2'-deoxyadenosine + H2O	6-aminopurine + 2-deoxy-D-ribofuranose		Camellia sinensis	-	6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside	137109	-	137109
2'-deoxyadenosine + H2O	6-aminopurine + 2-deoxy-D-ribofuranose		Brassica oleracea	-	6-aminopurine-9-2'-deoxy-beta-D-ribofuranoside	137113	-	137113
adenosine + H2O	6-aminopurine + D-ribofuranose		Lupinus luteus	-	component of the purine salvage pathway	137099	-	137099
adenosine + H2O	6-aminopurine + D-ribofuranose		Camellia sinensis	-	component of the purine salvage pathway	137101	-	137101
adenosine + H2O	6-aminopurine + D-ribofuranose		Camellia sinensis	-	component of the purine salvage pathway	137109	-	137109
adenosine + H2O	6-aminopurine + D-ribofuranose		Crithidia fasciculata, Trypanosoma cruzi	-	component of the purine salvage pathway	137108	-	137108
adenosine + H2O	6-aminopurine + D-ribofuranose		Solanum lycopersicum	-	component of the purine salvage pathway	137103	-	137103
adenosine + H2O	6-aminopurine + D-ribofuranose		Triticum aestivum	-	implicated in metabolism of cytokinins	137107	-	137107
adenosine + H2O	6-aminopurine + D-ribofuranose		Solanum lycopersicum	-	implicated in metabolism of cytokinins	137103	-	137103
adenosine + H2O	6-aminopurine + D-ribofuranose		Medicago sativa	-	involved in maintaining an active pool of S-adenosyl-L-methionine for transmethylation reactions	137100	-	137100
adenosine + H2O	6-aminopurine + D-ribofuranose		Triticum aestivum	-	6-aminopurine-9-beta-D-ribofuranoside	137107	-	137107
adenosine + H2O	6-aminopurine + D-ribofuranose		Lupinus luteus	-	6-aminopurine-9-beta-D-ribofuranoside	137099	-	137099
adenosine + H2O	6-aminopurine + D-ribofuranose		Lupinus luteus	-	6-aminopurine-9-beta-D-ribofuranoside	137110	-	137110
adenosine + H2O	6-aminopurine + D-ribofuranose		Hordeum vulgare	-	6-aminopurine-9-beta-D-ribofuranoside	137111	-	137111
adenosine + H2O	6-aminopurine + D-ribofuranose		Hordeum vulgare	-	6-aminopurine-9-beta-D-ribofuranoside	137102	-	137102
adenosine + H2O	6-aminopurine + D-ribofuranose		Hordeum vulgare	-	6-aminopurine-9-beta-D-ribofuranoside	137106	-	137106
adenosine + H2O	6-aminopurine + D-ribofuranose		Solanum tuberosum	-	6-aminopurine-9-beta-D-ribofuranoside	137112	-	137112
adenosine + H2O	6-aminopurine + D-ribofuranose		Camellia sinensis	-	6-aminopurine-9-beta-D-ribofuranoside	137101	-	137101
adenosine + H2O	6-aminopurine + D-ribofuranose		Camellia sinensis	-	6-aminopurine-9-beta-D-ribofuranoside	137109	-	137109
adenosine + H2O	6-aminopurine + D-ribofuranose		Crithidia fasciculata	-	6-aminopurine-9-beta-D-ribofuranoside	137108	-	137108
adenosine + H2O	6-aminopurine + D-ribofuranose		Medicago sativa	-	6-aminopurine-9-beta-D-ribofuranoside	137100	-	137100
adenosine + H2O	6-aminopurine + D-ribofuranose		Trypanosoma cruzi	-	6-aminopurine-9-beta-D-ribofuranoside	137108	-	137108
adenosine + H2O	6-aminopurine + D-ribofuranose		Solanum lycopersicum	-	6-aminopurine-9-beta-D-ribofuranoside	137103	-	137103
adenosine + H2O	6-aminopurine + D-ribofuranose		Helianthus tuberosus	-	6-aminopurine-9-beta-D-ribofuranoside	137051	-	137051
adenosine + H2O	6-aminopurine + D-ribofuranose		Brassica oleracea	-	6-aminopurine-9-beta-D-ribofuranoside	137113	-	137113
adenosine + H2O	6-aminopurine + D-ribofuranose		Fraxinus excelsior, Larix decidua, Tilia cordata, Ulmus carpinifolia, Acacia dealbata	-	6-aminopurine-9-beta-D-ribofuranoside	137104	-	137104
additional information	?	-	Pemphis acidula	-	30% of adenosine taken up by leaf disks is hydrolysed to adenine	657086	-	-
additional information	?	-	Lumnitzera racemosa	-	30% of adenosine taken up by leaf disks may be converted to salvage products	657086	-	-
additional information	?	-	Avicennia marina, Bruguiera gymnorhiza, Rhizophora stylosa, Kandelia candel, Sonneratia alba	-	56-92% of adenosine taken up by leaf disks may be converted to salvage products, efficient adenosine salvage abilitiy in mangrove is coupled to high enzymic activity and is stimulated by salt, proposed metabolic pathway	657086	-	-
additional information	?	-	Camellia sinensis	-	proposed pathway for biosynthesis of purine alkaloids with adenosine derived from SAM cycle	655545	-	-

COFACTOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

METALS and IONS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

INHIBITORS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
2'-deoxyadenosine	Triticum aestivum	-	one third inhibition at 0.5 mM	137107	2D-image
2'-deoxyadenosine	Hordeum vulgare	-	Ki: 0.05 mM	137111	2D-image
3'-Deoxyadenosine	Hordeum vulgare	-	Ki: four times weaker than for 2'-deoxyadenosine	137111	2D-image
6-Methylaminopurine	Crithidia fasciculata, Trypanosoma cruzi	-	growth inhibition by addition to culture media	137108	2D-image
adenine	Camellia sinensis	-	enzyme II inhibited at 0.2 mM, enzymes I and III not inhibited up to 1 mM	137109	2D-image
adenine	Hordeum vulgare	-	strong competitive inhibitor	137111	2D-image
adenine	Solanum tuberosum	-	64% inhibition at 4 mM	137112	2D-image
adenine	Brassica oleracea	-	38% inhibition at 9 mM	137113	2D-image
adenosine	Triticum aestivum	-	78% inhibition at 0.5 mM	137107	2D-image
Cu2+	Hordeum vulgare	-	marked inhibition at 1 mM	137102	2D-image
cytokinin	Hordeum vulgare	-	plant hormones, inhibition less strong than for adenine	137111	2D-image
dithiothreitol	Hordeum vulgare	-	60% inhibition if enzyme is preincubated with 10 mM	137111	2D-image
HgCl2	Hordeum vulgare	-	strong inhibition at 1 mM	137102	2D-image
Inosine	Solanum lycopersicum	-	at 0.2 mM: 8.5% inhibition for form Lf, 10.5% inhibition for form R2, form R1 not inhibited	137103	2D-image
N6-Benzyladenosine	Solanum lycopersicum	-	36.5% inhibition for form R1, 1.6% for form R2, form Lf not inhibited at 0.2 mM	137103	2D-image
N6-Benzyladenosine	Triticum aestivum	-	53% inhibition at 0.5 mM	137107	2D-image
N6-furfuryladenosine	Triticum aestivum	-	53% inhibition at 0.5 mM	137107	2D-image
N6-isopentenyladenosine	Solanum lycopersicum	-	inhibition between 28.6% and 36.7% for forms R1, R2 and Lf at 0.2 mM	137103	2D-image
p-chloromercuribenzoate	Hordeum vulgare	-	20% inhibition at 1 mM	137102	2D-image
trans-6-(4-hydroxy-3-methyl-2-butenylamino)-9-beta-D-ribofuranosylpurine	Triticum aestivum	-	70% inhibition at 0.5 mM	137107	2D-image
zeatin riboside	Solanum lycopersicum	-	competitive inhibition between 32% and 25% for forms R1, R2 and Lf at 0.2 mM	137103	2D-image
Zn2+	Hordeum vulgare	-	marked inhibition at 1 mM	137102	2D-image

ACTIVATING COMPOUND	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

KM VALUE [mM]	KM VALUE [mM] Maximum	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
0.12	-	2'-deoxyadenosine	Hordeum vulgare	-	in MES buffer, pH 5.4	137111	2D-image
0.001	-	adenosine	Triticum aestivum	-	-	137107	2D-image
0.001	-	adenosine	Hordeum vulgare	-	in MES buffer, pH 5.4	137111	2D-image
0.002	-	adenosine	Brassica oleracea	-	-	137113	2D-image
0.005	-	adenosine	Lupinus luteus	-	-	137099	2D-image
0.006	-	adenosine	Solanum lycopersicum	-	from Lf	137103	2D-image
0.0063	-	adenosine	Coffea arabica	-	-	670512	2D-image
0.009	-	adenosine	Solanum lycopersicum	-	form R2	137103	2D-image
0.017	-	adenosine	Helianthus tuberosus	-	-	137051	2D-image
0.025	-	adenosine	Solanum lycopersicum	-	form R1	137103	2D-image
0.27	-	adenosine	Hordeum vulgare	-	-	137102	2D-image
0.002	-	N6-(DELTA2-isopentenyl)-adenosine	Triticum aestivum	-	-	137107	2D-image
0.7	-	adenosine	Arabidopsis thaliana	-	recombinant enzyme expressed in Escherichia coli	700741	2D-image
additional information	-	additional information	Arabidopsis thaliana	-	kinetics	700741	-

TURNOVER NUMBER [1/s]	TURNOVER NUMBER MAXIMUM[1/s]	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
0.06	-	adenosine	Lupinus luteus	-	-	137099	2D-image

kcat/KM VALUE [1/mMs^-1]	kcat/KM VALUE [1/mMs^-1] Maximum	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

Ki VALUE [mM]	Ki VALUE [mM] Maximum	INHIBITOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
0.05	-	2'-deoxyadenosine	Hordeum vulgare	-	-	137111	2D-image

IC50 VALUE [mM]	IC50 VALUE [mM] Maximum	INHIBITOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg]	SPECIFIC ACTIVITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
0.003	-	Lupinus luteus	-	first activity after 2 days of seedling growth	137110
0.049	-	Lupinus luteus	-	maximum activity after 9 days of seedling growth	137110
0.62	-	Brassica oleracea	-	-	137113
0.74	-	Triticum aestivum	-	-	137107
3.2	-	Hordeum vulgare	-	-	137102
4	-	Camellia sinensis	-	-	137101
6.6	-	Lupinus luteus	-	-	137099
7	-	Camellia sinensis	-	for enzyme I	137109
7.97	-	Camellia sinensis	-	for enzyme III	137109
10	-	Hordeum vulgare	-	-	137106
10.6	-	Solanum tuberosum	-	-	137112
12.84	-	Camellia sinensis	-	for enzyme II	137109
30.5	-	Hordeum vulgare	-	-	137111
500	-	Coffea arabica	-	-	670512

pH OPTIMUM	pH MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
3.5	4.5	Brassica oleracea	-	optimum activity varies with buffer used	137113
4	-	Camellia sinensis	-	for enzymes I and III	137109
4	-	Solanum tuberosum	-	in acetate buffer	137112
4.5	-	Camellia sinensis	-	for enzyme II	137109
4.7	-	Triticum aestivum	-	-	137107
4.7	-	Hordeum vulgare	-	in 40 mM citrate buffer	137111
5	-	Hordeum vulgare	-	same activity in Tris or phosphate buffer, no requirement for inorganic phosphate	137102
5	-	Solanum lycopersicum	-	for form R1	137103
5.4	-	Hordeum vulgare	-	in 50 mM MES/KOH buffer	137111
6	7	Solanum tuberosum	-	in phosphate buffer	137112
6	-	Solanum lycopersicum	-	forms R2 and Lf	137103
6	-	Coffea arabica	-	50 mM citrate-phosphate buffer	670512
7.5	-	Lupinus luteus	-	-	137099

pH RANGE	pH RANGE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
5	7	Helianthus tuberosus	-	-	137051

TEMPERATURE OPTIMUM	TEMPERATURE OPTIMUM MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
45	-	Camellia sinensis	-	for enzyme I	137109
50	-	Camellia sinensis	-	for enzymes II and III	137109
58	-	Hordeum vulgare	-	-	137111
60	-	Hordeum vulgare	-	-	137102

TEMPERATURE RANGE	TEMPERATURE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
30	60	Hordeum vulgare	-	-	137102

pI VALUE	pI VALUE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

SOURCE TISSUE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	SOURCE
cell culture	Medicago sativa	-	rapid increase of activity following fungal elicitor treatment	137100
cell culture	Crithidia fasciculata, Trypanosoma cruzi	-	growth inhibition by 6-methylaminopurine	137108
cell suspension culture	Catharanthus roseus	-	transcript level of adenosine nucleosidase is reduced by the addition of Pi	681129
cell suspension culture	Catharanthus roseus	-	activity is maintained at a high level in long-term Pi starved cells	682289
cotyledon	Lupinus luteus	-	-	137099, 137110
germ	Triticum aestivum	-	-	137107
guard cell	Arabidopsis thaliana	-	-	700741, 710272
leaf	Medicago sativa	-	enzyme synthesis greater than in cell culture	137100
leaf	Camellia sinensis	-	-	137101, 137109
leaf	Solanum lycopersicum	-	form Lf	137103
leaf	Acacia dealbata, Fraxinus excelsior, Larix decidua, Tilia cordata, Ulmus carpinifolia	-	-	137104
leaf	Hordeum vulgare	-	-	137106, 137111
leaf	Solanum tuberosum	-	-	137112
leaf	Camellia sinensis	-	activity decreases rapidly during aging of leaf	655545
leaf	Avicennia marina, Bruguiera gymnorhiza, Kandelia candel, Lumnitzera racemosa, Pemphis acidula, Rhizophora stylosa, Sonneratia alba	-	-	657086
leaf	Coffea arabica	-	young	670512
pollen	Arabidopsis thaliana	-	-	700741, 710272
root	Medicago sativa	-	enzyme synthesis greater than in leaves	137100
root	Solanum lycopersicum	-	two forms, R1 and R2	137103
root	Arabidopsis thaliana	-	-	700741, 710272
root tip	Arabidopsis thaliana	-	-	700741, 710272
seed	Medicago sativa	-	-	137100
seedling	Hordeum vulgare	-	malt	137102
shoot	Helianthus tuberosus	-	-	137051
sprout	Brassica oleracea	-	-	137113
tuber	Solanum tuberosum	-	adenosine nucleosidase activity is negligible in freshly prepared slices, activity is dramatically enhanced in wounded slices	676653
vascular cell	Arabidopsis thaliana	-	of roots and in root tips	700741, 710272

LOCALIZATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	GeneOntology No.	LITERATURE	SOURCE
chloroplast	Camellia sinensis	-	-	9507	655545
cytoplasm	Arabidopsis thaliana	-	-	5737	710272

PDB	SCOP	CATH	ORGANISM
No entries in this field

MOLECULAR WEIGHT	MOLECULAR WEIGHT MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
59000	-	Triticum aestivum	-	gel filtration	137107
62400	-	Solanum tuberosum	-	sucrose density gradient centrifugation, gel filtration	137112
66000	-	Hordeum vulgare	-	gel filtration	137111
68000	-	Solanum lycopersicum	-	gel filtration	137103
68000	-	Camellia sinensis	-	for enzymes I, II and III	137109
72000	-	Lupinus luteus	-	gel filtration	137099
72000	-	Coffea arabica	-	gel filtration	670512
120000	-	Hordeum vulgare	-	gel filtration	137102

SUBUNITS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
dimer	Lupinus luteus	-	2 * 33000-36000, SDS-PAGE	137099
dimer	Hordeum vulgare	-	alpha2, 2 * 33000, non-reducing SDS-PAGE	137111
dimer	Coffea arabica	-	2 * 34600, SDS-PAGE	670512

POSTTRANSLATIONAL MODIFICATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

Crystallization/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

pH STABILITY	pH STABILITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
6	8	Camellia sinensis	-	stable within, enzymes I, II and III	137109
9	-	Helianthus tuberosus	-	50% activity reatained	137051

TEMPERATURE STABILITY	TEMPERATURE STABILITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
10	-	Brassica oleracea	-	stable for 15 days	137113
40	-	Camellia sinensis	-	rapid loss of activity, enzymes I, II and III	137109
60	-	Hordeum vulgare	-	activity sharply decreases above	137102
115	-	Hordeum vulgare	-	40% remaining activity after 15 min for Sephadex G-50 stabilized enzyme	137106

GENERAL STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
stable against repeated freezing and thawing cycles	Brassica oleracea	-	137113
highly stable at 4�C and under deep-freeze conditions, no reducing agent required for stability	Helianthus tuberosus	-	137051
labile during course of purification, addition of dithiothreitol required, 50% loss of activity if stored at -20�C in one week	Hordeum vulgare	-	137102
presence of salt essential	Hordeum vulgare	-	137111
stable against repeated freezing and thawing cycles	Hordeum vulgare	-	137111
stable against repeated freezing and thawing cycles	Lupinus luteus	-	137099

ORGANIC SOLVENT	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
Methanol	Lupinus luteus	-	remains active up to 20%, v/v	137099

OXIDATION STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

STORAGE STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
-10�C, 10 mM NaHCO3, 4 months	Brassica oleracea	-	137113
-20�C, one month, gradually loss of activity on storage at 4�C	Camellia sinensis	-	137109
4�C, 20 mM phosphate, pH 7.2, 10 mM 2-mercaptoethanol, 70% activity remains, 12 weeks	Hordeum vulgare	-	137102
long-term storage at room temperature for Sephadex G-50 stabilized enzyme, 100% activity remains after 4.5 years	Hordeum vulgare	-	137106
storage in dried state in Sephadex G-25 beads remains stability for several months	Hordeum vulgare	-	137111
-20�C, 95% retaining activity after 30 days	Triticum aestivum	-	137107

Purification/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
partial	Brassica oleracea	-	137113
partial	Camellia sinensis	-	137101
partial, three forms isolated: enzymes I, II and III	Camellia sinensis	-	137109
-	Coffea arabica	-	670512
partial	Hordeum vulgare	-	137106
to homogeneity, chromatography steps	Hordeum vulgare	-	137102, 137111
to homogeneity, chromatography steps	Lupinus luteus	-	137099
form R2 to homogeneity, forms R1 and Lf partial	Solanum lycopersicum	-	137103
near homogeneity	Solanum tuberosum	-	137112
partial	Triticum aestivum	-	137107

Cloned/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
URH1, DNA and amino acid sequence determination and analysis, expression in Escherichia coli, functional complementation of a yeast mutant, expression in transgenic Arabidopsis thaliana mutant plants under control of the CaMV 35S promoter	Arabidopsis thaliana	-	700741

EXPRESSION	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

ENGINEERING	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
additional information	Arabidopsis thaliana	-	mutants expressing the Arabidopsis thaliana enzyme or the homologue from Oryza sativa exhibit resistance toward toxic fluorouridine, fluorouracil, and fluoroorotic acid. Mutants with increased and decreased nucleosidase activity are delayed in germination	700741

Renatured/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

APPLICATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

REF.	AUTHORS	TITLE	JOURNAL	VOL.	PAGES	YEAR	ORGANISM	LINK TO PUBMED	SOURCE
137051	Le Floc'h, F.; Lafleuriel, J.	The purine nucleosidases of Jerusalem artichoke shoots	Phytochemistry	20	2127-2129	1981	Helianthus tuberosus	-
137099	Abusamhadneh, E.; McDonald, N.E.; Kline, P.C.	Isolation and characterization of adenosine nucleosidase from yellow lupin (Lupinus luteus)	Plant Sci.	153	25-32	2000	Lupinus luteus	-
137100	Edwards, R.	S-adenosyl-L-methionine metabolism in alfalfa cell cultures following treatment with fungal elicitors	Phytochemistry	43	1163-1169	1996	Medicago sativa	-
137101	Negishi, O.; Ozawa, T.; Imagawa, H.	N-methyl nucleosidase from tea leaves	Agric. Biol. Chem.	52	169-175	1988	Camellia sinensis	-
137102	Lee, W.J.; Pyler, R.E.	Nucleic acid degrading enzymes of barley malt. III. Adenosine nucleosidase from malted barley	J. Am. Soc. Brew. Chem.	44	86-90	1986	Hordeum vulgare	-
137103	Burch, L.R.; Stuchbury, T.	Purification and properties of adenosine nucleosidases from tomato (Lycopersicon esculentum) roots and leaves	J. Plant Physiol.	125	267-273	1986	Solanum lycopersicum	-
137104	Leszczynska, D.; Schneider, Z.; Tomaszewski, M.; Mackowiak, M.	Comparative studies on adenosine nucleosidase occurrence in plants	Ann. Bot.	54	847-849	1984	Acacia dealbata, Fraxinus excelsior, Larix decidua, no activity in Ceratodon purpureus, no activity in Equisetum arvense, no activity in Populus alba, Tilia cordata, Ulmus carpinifolia	-
137106	Schneider, Z.; Friedmann, H.C.	Stabilization of enzymes in Sephadex - a long-term experiment	J. Appl. Biochem.	3	135-146	1981	Hordeum vulgare	-
137107	Chen, C.M.; Kristopeit, S.M.	Metabolism of cytokinin: Deribosylation of cytokinin ribonucleoside by adenosine nucleosidase from wheat germ cells	Plant Physiol.	68	1020-1023	1981	Triticum aestivum	PubMed
137108	Nolan, L.L.; Kidder, G.W.	Inhibition of growth and purine-metabolizing enzymes of trypanosomid flagellates by N6-methyladenine	Antimicrob. Agents Chemother.	17	567-571	1980	Crithidia fasciculata, no activity in Leishmania braziliensis, no activity in Leishmania donovani, no activity in Leishmania mexicana, no activity in Leishmania tarentolae, Trypanosoma cruzi	PubMed
137109	Imagawa, H.; Yamano, H.; Inoue, K.; Takino, Y.	Purification and properties of adenosine nucleosidases from tea leaves	Agric. Biol. Chem.	43	2337-2342	1979	Camellia sinensis	-
137110	Guranowski, A.; Pawelkiewicz, J.	Adenosylhomocysteinase and adenosine nucleosidase activities in Lupinus luteus cotyledons during seed formation and germination	Planta	139	245-247	1978	Lupinus luteus	-
137111	Guranowski, A.; Schneider, Z.	Purification and characterization of adenosine nucleosidase from barley leaves	Biochim. Biophys. Acta	482	145-158	1977	Hordeum vulgare	PubMed
137112	Clark, M.C.; Page, O.T.; Fisher, M.G.	Purification and properties of N-ribosyladenine ribohydrolase from potato leaves	Phytochemistry	11	3413-3419	1972	Solanum tuberosum	-
137113	Mazelis, M.; Creveling, R.K.	An adenosine hydrolase from Brussels sprouts	J. Biol. Chem.	238	3358-3361	1963	Brassica oleracea	PubMed
655545	Koshiishi, C.; Kato, A.; Yama, S.; Crozier, A.; Ashihara, H.	A new caffeine biosynthetic pathway in tea leaves: utilisation of adenosine released from the S-adenosyl-L-methionine cycle	FEBS Lett.	499	50-54	2001	Camellia sinensis	PubMed
657086	Ashihara, H.; Wakahara, S.; Suzuki, M.; Kato, A.; Sasamoto, H.; Baba, S.	Comparison of adenosine metabolism in leaves of several mangrove plants and a poplar species	Plant Physiol. Biochem.	41	133-139	2003	Avicennia marina, Bruguiera gymnorhiza, Kandelia candel, Lumnitzera racemosa, Pemphis acidula, Rhizophora stylosa, Sonneratia alba	-
670512	Campos, A.; Rijo-Johansen, M.J.; Carneiro, M.F.; Fevereiro, P.	Purification and characterisation of adenosine nucleosidase from Coffea arabica young leaves	Phytochemistry	66	147-151	2005	Coffea arabica	PubMed
676653	Katahira, R.; Ashihara, H.	Role of adenosine salvage in wound-induced adenylate biosynthesis in potato tuber slices	Plant Physiol. Biochem.	44	551-555	2006	Solanum tuberosum	PubMed
681129	Yin, Y.; Shimano, F.; Ashihara, H.	Involvement of rapid nucleotide synthesis in recovery from phosphate starvation of Catharanthus roseus cells	J. Exp. Bot.	58	1025-1033	2007	Catharanthus roseus	PubMed
682289	Shimano, F.; Ashihara, H.	Effect of long-term phosphate starvation on the levels and metabolism of purine nucleotides in suspension-cultured Catharanthus roseus cells	Phytochemistry	67	132-141	2006	Catharanthus roseus	PubMed
700741	Jung, B.; Floerchinger, M.; Kunz, H.H.; Traub, M.; Wartenberg, R.; Jeblick, W.; Neuhaus, H.E.; Moehlmann, T.	Uridine-ribohydrolase is a key regulator in the uridine degradation pathway of Arabidopsis	Plant Cell	21	876-891	2009	Arabidopsis thaliana	PubMed
710272	Mach, J.	Uridine ribohydrolase and the balance between nucleotide degradation and salvage	Plant Cell	21	0000	2009	Arabidopsis thaliana	-

LINKS TO OTHER DATABASES (specific for EC-Number 3.2.2.7)

NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM

IUBMB Enzyme Nomenclature

PROSITE Database of protein families and domains

SYSTERS

Protein Mutant Database

InterPro (database of protein families, domains and functional sites)

All organisms
Acacia dealbata
Arabidopsis thaliana
Avicennia marina
Brassica oleracea
Bruguiera gymnorhiza
Camellia sinensis
Catharanthus roseus
Coffea arabica
Crithidia fasciculata
Fraxinus excelsior
Helianthus tuberosus
Hordeum vulgare
Kandelia candel
Larix decidua
Lumnitzera racemosa
Lupinus luteus
Medicago sativa
Pemphis acidula
Rhizophora stylosa
Solanum lycopersicum
Solanum tuberosum
Sonneratia alba
Tilia cordata
Triticum aestivum
Trypanosoma cruzi
Ulmus carpinifolia