Information on EC 3.2.1.80 - fructan beta-fructosidase:
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EC NUMBERCOMMENTARY
3.2.1.80-

RECOMMENDED NAMEGeneOntology No.
fructan beta-fructosidaseGO:0051669

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
Hydrolysis of terminal, non-reducing (2->1)- and (2->6)-linked beta-D-fructofuranose residues in fructans
show the reaction diagram		----
Hydrolysis of terminal, non-reducing (2->1)- and (2->6)-linked beta-D-fructofuranose residues in fructans
show the reaction diagram		exclusive hydrolysis of beta-2-6 bonds in fructan-producing plantsTriticum aestivum-669668
Hydrolysis of terminal, non-reducing (2->1)- and (2->6)-linked beta-D-fructofuranose residues in fructans
show the reaction diagram		-Cichorium intybus-670976

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
hydrolysisCryptococcus aureus-of inulin690488
hydrolysis of O-glycosyl bond----
hydrolysis of O-glycosyl bondAspergillus ficuum--690509

PATHWAYKEGG LinkMetaCyc Link
fructan degradation-PWY-862

SYSTEMATIC NAMEIUBMB Comments
beta-D-fructan fructohydrolaseHydrolyses inulin and levan, and also sucrose.

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
(6&1) FEHArabidopsis thaliana--670539
1-FEHCichorium intybusQ93X60-660179
1-FEHAllium cepa--665885
1-FEHVernonia herbaceaA9JIF3; 670618
1-FEHSmallanthus sonchifolius--681161
1-FEHArabidopsis thalianaQ67XZ3-682426
1-FEHTriticum aestivum--705947
1-FEHArctium lappa--714715
1-FEH ICichorium intybus--656993
1-FEH IIaCichorium intybus--656406, 682143
1-FEH IIaCichorium intybus-; 682145
1-FEHaLolium perenneQ64GB3-681133
1-KEHArabidopsis thalianaQ67XZ3-682426
1-kestose exohydrolaseArabidopsis thalianaQ67XZ3-682426
2,1-beta-D-fructan fructohydrolaseAspergillus awamoriQ96TU3-654875
6&1-FEH w1Triticum aestivum--668724
6-FEHBeta vulgaris--657006, 670976
6-FEHTriticum aestivum--669668
AtcwINV1 D239A mutantArabidopsis thalianaQ67XZ3-682426
AtcwINV3Arabidopsis thaliana--670539
AtcwINV6Arabidopsis thaliana--670539
beta-D-fructan fructanohydrolaseKluyveromyces marxianus--678762
beta-D-fructan fructanohydrolaseAspergillus niger--679966
Ci1-1-FEH IIaCichorium intybus--682147
EC 3.2.1.153Lolium perenneQ64GB3formerly681133
exo-beta-D-fructosidase----
exo-beta-D-fructosidaseAspergillus nigerQ96WZ8-657274
exo-beta-fructosidase----
exoinulinaseAspergillus fumigatus--654218
exoinulinaseAspergillus awamoriQ96TU3-654875
exoinulinasePenicillium sp.--655019, 680946
exoinulinaseAspergillus ficuum--655298, 690509
exoinulinaseAlternaria alternata--655714
exoinulinaseAspergillus nigerQ96WZ8-657274
exoinulinaseBacillus sp.--657429
exoinulinaseMeyerozyma guilliermondii--681217
exoinulinaseKluyveromyces marxianus--681357
exoinulinaseCryptococcus aureus--690488
FEHTriticum aestivum--660316
FEHArabidopsis thaliana, Cichorium intybus--670976
FEH1Phleum pratense--715762
fructan 1-exohydrolaseTriticum aestivum--657048
fructan 1-exohydrolaseVernonia herbaceaA9JIF3-670618
fructan 1-exohydrolaseLolium perenneQ64GB3-681133
fructan 1-exohydrolaseSmallanthus sonchifolius--681161
fructan 1-exohydrolaseCichorium intybusQ93X60-682143, 682145, 682147
fructan 1-exohydrolaseArabidopsis thalianaQ67XZ3-682426
fructan 1-exohydrolaseArctium lappa--714715
fructan 1-exohydrolasePhleum pratense--715762
fructan 1-exohydrolase ICichorium intybus--656993
fructan 1-exohydrolase IICichorium intybus--657033
fructan 1-exohydrolase IIaCichorium intybus--654116
fructan 6-exohydrolaseBeta vulgaris--657006
fructan beta-fructosidaseLactobacillus pentosus--657381
fructan exohydrolase----
fructan exohydrolaseTriticum aestivum--705947, 716041
Fructanase----
FructanaseKluyveromyces marxianus--714499
fructofuranosidase, polysaccharide beta----
fructofuranosyl hydrolaseAspergillus ficuum--690509
glycoside hydrolaseAspergillus ficuum-family 32690509
inuDPenicillium sp.Q8J0G1-680946
inuEPenicillium sp.--680950
inulinaseAspergillus niger--679966
inulinaseCryptococcus aureus--690488
levanaseBeta vulgaris--657006
P-IPenicillium sp.Q8J0G1-655019
polysaccharide beta-fructofuranosidase----
sacCpBacillus subtilisP05656-714493
Wfh-sm1Triticum aestivum-gene name716041

CAS REGISTRY NUMBERCOMMENTARY
37288-56-5-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Actinomyces naeslundiiT14V136519--Manually annotated by BRENDA team
Allium cepava Tenshin665885--Manually annotated by BRENDA team
Alternaria alternata(Fr.) Keissler655714--Manually annotated by BRENDA team
Arabidopsis thaliana-670539, 670976--Manually annotated by BRENDA team
Arabidopsis thaliana-682426Q67XZ3UniprotManually annotated by BRENDA team
Arctium lappa-714715--Manually annotated by BRENDA team
Aspergillus awamoriEI669841--Manually annotated by BRENDA team
Aspergillus awamorivar. 2250654875Q96TU3SwissProtManually annotated by BRENDA team
Aspergillus awamori EIEI669841--Manually annotated by BRENDA team
Aspergillus ficuum-690509--Manually annotated by BRENDA team
Aspergillus ficuumJNSP5-06655298--Manually annotated by BRENDA team
Aspergillus fumigatus-654218--Manually annotated by BRENDA team
Aspergillus nigerAF10657274Q96WZ8SwissProtManually annotated by BRENDA team
Aspergillus nigerstrain SL-09679966--Manually annotated by BRENDA team
Aspergillus niger AF10AF10657274Q96WZ8SwissProtManually annotated by BRENDA team
Aspergillus niger SL-09strain SL-09679966--Manually annotated by BRENDA team
Avena sativaoat208823--Manually annotated by BRENDA team
Bacillus sp.strain 11657429--Manually annotated by BRENDA team
Bacillus subtilis-714493P05656UniProtManually annotated by BRENDA team
Beta vulgaris-670976--Manually annotated by BRENDA team
Beta vulgarissugar beet657006--Manually annotated by BRENDA team
Candida albicans 11strain 11657429--Manually annotated by BRENDA team
Cichorium intybus-208825, 654116, 656993, 670976, 682145--Manually annotated by BRENDA team
Cichorium intybus-656406Q93X60SwissProtManually annotated by BRENDA team
Cichorium intybusBelgian endive657033--Manually annotated by BRENDA team
Cichorium intybusisoenzyme 1-FEH IIa660179Q93X60SwissProtManually annotated by BRENDA team
Cichorium intybusprecursor682143, 682145, 682147Q93X60SwissProtManually annotated by BRENDA team
Cryptococcus aureusG7a681774--Manually annotated by BRENDA team
Cryptococcus aureusstrain G7a, marine yeast, isolated from sediment at China South See690488--Manually annotated by BRENDA team
Cryptococcus aureus G7aG7a681774--Manually annotated by BRENDA team
Debaryomyces hanseniiG7a1681774--Manually annotated by BRENDA team
Debaryomyces hansenii G7a1G7a1681774--Manually annotated by BRENDA team
Helianthus tuberosusJerusalem artichoke208819--Manually annotated by BRENDA team
Hordeum vulgarebarley208824--Manually annotated by BRENDA team
Kluyveromyces marxianus-714499--Manually annotated by BRENDA team
Kluyveromyces marxianusstrain YS-1678752, 681357--Manually annotated by BRENDA team
Kluyveromyces marxianusvar. bulgaricus679967--Manually annotated by BRENDA team
Kluyveromyces marxianusYS-1678762--Manually annotated by BRENDA team
Kluyveromyces marxianus YS-1 Ys-1YS-1678762--Manually annotated by BRENDA team
Lactobacillus pentosusB235657381--Manually annotated by BRENDA team
Lactobacillus pentosus B235B235657381--Manually annotated by BRENDA team
Lolium perennevariant Bravo681133Q64GB3SwissProtManually annotated by BRENDA team
Meyerozyma guilliermondii-681217--Manually annotated by BRENDA team
Meyerozyma guilliermondiiOUC1681774--Manually annotated by BRENDA team
Meyerozyma guilliermondii OUC1OUC1681774--Manually annotated by BRENDA team
Penicillium janczewskii-682058--Manually annotated by BRENDA team
Penicillium sp.precursor; strain TN-88680946Q8J0G1UniProtManually annotated by BRENDA team
Penicillium sp.strain TN-88655019Q8J0G1UniProtManually annotated by BRENDA team
Penicillium sp.strain TN-88680950--Manually annotated by BRENDA team
Penicillium sp. TN-88strain TN-88655019Q8J0G1UniProtManually annotated by BRENDA team
Phleum pratensecultivar Hokushu715762--Manually annotated by BRENDA team
Smallanthus sonchifolius-681161--Manually annotated by BRENDA team
Streptococcus mutansGS-5208817, 208821--Manually annotated by BRENDA team
Streptococcus mutansingbritt208820--Manually annotated by BRENDA team
Streptococcus mutans GS-5GS-5208817--Manually annotated by BRENDA team
Streptococcus salivarius-208818--Manually annotated by BRENDA team
Triticum aestivum-660316, 668724, 716041--Manually annotated by BRENDA team
Triticum aestivumcv. Pajero669668--Manually annotated by BRENDA team
Triticum aestivumdrought-resistant varieties Kauz and Westonia705947--Manually annotated by BRENDA team
Triticum aestivumwinter wheat657048--Manually annotated by BRENDA team
Vernonia herbacea-670618--Manually annotated by BRENDA team
Vernonia herbaceafragment670618A9JIF3UniProtManually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
1,1,1-kestopentaose + H2O?
show the reaction diagram		Phleum pratense-18% activity compared to levan715762--?
1,1,1-kestopentaose + H2Obeta-D-fructose + ?
show the reaction diagram		Triticum aestivum--668724--?
1,1,1-kestose + H2Obeta-D-fructose + sucrose
show the reaction diagram		Lactobacillus pentosus-45% enzyme activity in comparison to levan657381--?
1,1-kestose + H2Obeta-D-fructose + sucrose
show the reaction diagram		Lactobacillus pentosus-39% enzyme activity in comparison to levan657381--?
1,1-kestotetraose + H2O?
show the reaction diagram		Lolium perenneQ64GB3100% activity681133--?
1,1-kestotetraose + H2O?
show the reaction diagram		Phleum pratense-18% activity compared to levan715762--?
1,1-kestotetraose + H2O?
show the reaction diagram		Triticum aestivum-gene product Wfh-sm1 shows 8% activity with 1,1-kestotetraose compared to sucrose716041--?
1,1-nystose + H2Obeta-D-fructose + sucrose
show the reaction diagram		Triticum aestivum--668724--?
1,1-nystose + H2Obeta-D-fructose + sucrose
show the reaction diagram		Triticum aestivum-72% activity of isoform w1 and 83% of w2 in comparison to substrate 1-kestose657048--?
1-kestose + H2O?
show the reaction diagram		Arabidopsis thalianaQ67XZ3-682426--?
1-kestose + H2O?
show the reaction diagram		Cichorium intybusQ93X60-682143--?
1-kestose + H2O?
show the reaction diagram		Smallanthus sonchifolius--681161--?
1-kestose + H2O?
show the reaction diagram		Arctium lappa-100% activity714715--?
1-kestose + H2Obeta D-fructofuranose + sucrose
show the reaction diagram		Cichorium intybus--682145--?
1-kestose + H2Obeta D-fructofuranose + sucrose
show the reaction diagram		Triticum aestivum-100% activity of both isoforms w1 and w2657048--?
1-kestose + H2Obeta-D-fructose + sucrose
show the reaction diagram		Triticum aestivum--668724--?
1-kestose + H2Obeta-D-fructose + sucrose
show the reaction diagram		Cichorium intybusQ93X60-682143--?
1-kestose + H2Obeta-D-fructose + sucrose
show the reaction diagram		Cichorium intybus--682145, 682147--?
1-kestose + H2Obeta-D-fructose + sucrose
show the reaction diagram		Lactobacillus pentosus-12% enzyme activity in comparison to levan657381--?
1-kestotriose + H2O?
show the reaction diagram		Lolium perenneQ64GB358% activity compared to 1,1-kestotetraose681133--?
1-kestotriose + H2O?
show the reaction diagram		Phleum pratense-7% activity compared to levan715762--?
1-kestotriose + H2O?
show the reaction diagram		Triticum aestivum-gene product Wfh-sm1 shows 40% activity with 1-kestotriose compared to sucrose716041--?
1-nystose + H2O?
show the reaction diagram		Smallanthus sonchifolius--681161--?
6-kestose + H2Obeta-D-fructose + sucrose
show the reaction diagram		Triticum aestivum--668724, 669668--?
6-kestose + H2Obeta-D-fructose + sucrose
show the reaction diagram		Triticum aestivum-0.7% activity of isoform w1 and 1% of w2 in comparison to substrate 1-kestose657048--?
6-kestose + H2O?
show the reaction diagram		Arctium lappa-2% activity compared to 1-kestose714715--?
6-kestotriose + H2O?
show the reaction diagram		Lolium perenneQ64GB36% activity compared to 1,1-kestotetraose681133--?
6-kestotriose + H2O?
show the reaction diagram		Phleum pratense-25% activity compared to levan715762--?
6-kestotriose + H2O?
show the reaction diagram		Triticum aestivum-gene product Wfh-sm1 shows 38% activity with 6-kestotriose compared to sucrose716041--?
6G,6-kestotetraose + H2OD-fructose + neokestose
show the reaction diagram		Avena sativa-100%, chicorium inulin 11.9%, Aerobacter levan 8.5%, sucrose 0% rate of hydrolysis208823-208823?
6G-kestotriose + H2O?
show the reaction diagram		Lolium perenneQ64GB319% activity compared to 1,1-kestotetraose681133--?
Actilight + H2O?
show the reaction diagram		Kluyveromyces marxianus-highest activity, Actilight is a commercial mixture composed of 4.8% (w/v) of free sugars (sucrose, glucose and fructose), 35.1% w/v of 1-kestose, 53% w/v of 1-nystose and 7.1% w/v of 1-fructofuranosyl-nystose714499--?
Agave tequilana fructan + H2O?
show the reaction diagram		Kluyveromyces marxianus--714499--?
burdock root sugar extract + H2Ofructose + sucrose + ?
show the reaction diagram		Arctium lappa--714715--?
fructan + H2Obeta-D-fructose + ?
show the reaction diagram		Beta vulgaris--670976--?
fructan + H2Obeta-D-fructose + ?
show the reaction diagram		Triticum aestivum--660316--?
fructan + H2Obeta-D-fructose + ?
show the reaction diagram		Arabidopsis thaliana, Cichorium intybus--670976--?
fructan + H2Obeta-D-fructose + ?
show the reaction diagram		Cichorium intybus--682145--?
fructans + H2OD-fructose
show the reaction diagram		Helianthus tuberosus-degradation208819--?
fructans + H2OD-fructose
show the reaction diagram		Streptococcus mutans-degradation208817--?
fructosylnystose + H2O?
show the reaction diagram		Arctium lappa-97% activity compared to 1-kestose714715--?
garlic extract + H2O?
show the reaction diagram		Lactobacillus pentosus-60% enzyme activity in comparison to levan657381--?
inulin + H2O?
show the reaction diagram		Kluyveromyces marxianus--678752--?
inulin + H2O?
show the reaction diagram		Cichorium intybus--682147--?
inulin + H2O?
show the reaction diagram		Bacillus subtilisP05656-714493--?
inulin + H2O?
show the reaction diagram		Meyerozyma guilliermondii-large amount of monosaccharides and a trace amount of oligosaccharide are produced681774--?
inulin + H2O?
show the reaction diagram		Meyerozyma guilliermondii-large amount of monosaccharides and a trace amount of oligosaccharides are detected after the hydrolysis681217--?
inulin + H2O?
show the reaction diagram		Debaryomyces hansenii, Cryptococcus aureus-large amount of monosaccharides and a trace amount of oligosaccharides are detected, exoinulinase activity681774--?
inulin + H2O?
show the reaction diagram		Arctium lappa-42% activity compared to 1-kestose714715--?
inulin + H2O?
show the reaction diagram		Triticum aestivum-gene product Wfh-sm1 shows less than 1% activity with inulin compared to sucrose716041--?
inulin + H2Ooligofructosides
show the reaction diagram		Aspergillus fumigatus--654218--?
inulin + H2Ooligofructosides
show the reaction diagram		Alternaria alternata--655714--?
inulin + H2OD-fructose
show the reaction diagram		Penicillium sp.--680950--?
inulin + H2OD-fructose
show the reaction diagram		Streptococcus mutans--208817-208817?
inulin + H2OD-fructose
show the reaction diagram		Streptococcus mutans--208820-208820?
inulin + H2OD-fructose
show the reaction diagram		Streptococcus salivarius--208818-208818?
inulin + H2OD-fructose
show the reaction diagram		Actinomyces naeslundii--136519--?
inulin + H2OD-fructose
show the reaction diagram		Cichorium intybus--208825---
inulin + H2OD-fructose
show the reaction diagram		Aspergillus ficuum--690509after incubation at 50°C for 1 h-?
inulin + H2OD-fructose
show the reaction diagram		Triticum aestivum-39% activity of isoform w1 and 22% of w2 in comparison to substrate 1-kestose657048--?
inulin + H2OD-fructose
show the reaction diagram		Kluyveromyces marxianus-root powder extract of Asparagus officinalis. Hydrolyzate product mainly consists of fructose and traces of glucose678762--?
inulin + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Kluyveromyces marxianus--714499--?
inulin + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Aspergillus ficuum--655298--?
inulin + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Cichorium intybus--682145--?
inulin + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Aspergillus awamoriQ96TU3-654875--?
inulin + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Aspergillus nigerQ96WZ8-657274--?
inulin + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Penicillium sp.Q8J0G1-655019--?
inulin + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Smallanthus sonchifolius--681161--?
inulin + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Bacillus sp.-no activity with melizitose657429--?
inulin + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Penicillium sp.Q8J0G1no activity with bacterial levan655019--?
inulin + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Lolium perenneQ64GB324% activity compared to 1,1-kestotetraose681133--?
inulin + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Phleum pratense-6% activity compared to levan715762--?
inulin + H2Obeta-D-fructose
show the reaction diagram		Aspergillus awamori--669841--?
inulin + H2Obeta-D-fructose
show the reaction diagram		Penicillium janczewskii--682058--?
inulin + H2Obeta-D-fructose + sucrose
show the reaction diagram		Vernonia herbaceaA9JIF3-670618--?
inulin + H2Obeta-D-fructose + sucrose
show the reaction diagram		Lactobacillus pentosus-9% enzyme activity in comparison to levan657381--?
inulin + H2Obeta-D-fructose + ?
show the reaction diagram		Arabidopsis thaliana--670539--?
inulin + H2Obeta-D-fructose + ?
show the reaction diagram		Cichorium intybusQ93X60-660179--?
inulin + H2OD-glucose + D-fructose
show the reaction diagram		Kluyveromyces marxianus--681357--?
inulin + H2OD-fructose + D-glucose + fructooligosaccharides
show the reaction diagram		Kluyveromyces marxianus-a typical solution product consists of a mixture of fructose (155 g/l), glucose (155 g/l), sucrose (132 g/l) and fructooligosaccharides (50 g/l)679967--?
inulin + H2O1-Kestose
show the reaction diagram		Aspergillus ficuum--690509--?
inulin + H2Obeta-D-Fruf-(2-1)-beta-D-Fruf
show the reaction diagram		Aspergillus ficuum--690509--?
inulin + H2OD-frucotse + fructooligosaccharides
show the reaction diagram		Cryptococcus aureus--690488crude inulinase-?
inulin + H2OD-fructose + D-glucose
show the reaction diagram		Cryptococcus aureus--690488--?
inulin + H2Osucrose
show the reaction diagram		Aspergillus ficuum--690509--?
inulin-type fructans (beta-2,1) + H2OD-fructose
show the reaction diagram		Beta vulgaris-poorly substrate657006--?
inulobiose + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Aspergillus awamoriQ96TU3inulin is an intermediate product654875--?
inulobiose + H2O?
show the reaction diagram		Arctium lappa-12% activity compared to 1-kestose714715--?
inuloheptaose + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Aspergillus awamoriQ96TU3inulohexaose, inulopentaose, inulotetraose, inulotriose, inulobiose and inulin are intermediate products654875--?
inulohexaose + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Aspergillus awamoriQ96TU3inulopentaose, inulotetraose, inulotriose, inulobiose and inulin are intermediate products654875--?
inulopentaose + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Aspergillus awamoriQ96TU3inulotetraose, inulotriose, inulobiose and inulin are intermediate products654875--?
inulotetraose + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Aspergillus awamoriQ96TU3inulotriose, inulobiose and inulin are intermediate products654875--?
inulotriose + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Aspergillus awamoriQ96TU3inulobiose and inulin are intermediate products654875--?
inulotriose + H2O?
show the reaction diagram		Arctium lappa-41% activity compared to 1-kestose714715--?
levan + H2O?
show the reaction diagram		Bacillus subtilisP05656-714493--?
levan + H2O?
show the reaction diagram		Triticum aestivum-gene product Wfh-sm1 shows less than 1% activity with levan compared to sucrose716041--?
levan + H2OD-fructose
show the reaction diagram		Streptococcus mutans--208817-208817?
levan + H2OD-fructose
show the reaction diagram		Streptococcus salivarius--208818-208818?
levan + H2OD-fructose
show the reaction diagram		Actinomyces naeslundii--136519--?
levan + H2OD-fructose
show the reaction diagram		Lactobacillus pentosus-100% enzyme activity657381--?
levan + H2OD-fructose
show the reaction diagram		Triticum aestivum-4% activity of isoform w1 and 3% of w2 in comparison to substrate 1-kestose657048--?
levan + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Aspergillus awamoriQ96TU3-654875--?
levan + H2Obeta-D-fructose
show the reaction diagram		Aspergillus awamori--669841--?
levan + H2Obeta-D-fructose + ?
show the reaction diagram		Beta vulgaris--670976--?
levan + H2Obeta-D-fructose + ?
show the reaction diagram		Triticum aestivum--668724, 669668--?
levan + H2Obeta-D-fructose + ?
show the reaction diagram		Arabidopsis thaliana, Cichorium intybus--670976--?
levan + H2Obeta-D-fructose + ?
show the reaction diagram		Phleum pratense-100% activity715762--?
levan + H2OD-fructose + ?
show the reaction diagram		Kluyveromyces marxianus--714499--?
levan + H2OD-fructose + ?
show the reaction diagram		Lolium perenneQ64GB30.5% activity compared to 1,1-kestotetraose681133--?
levan-type fructan + H2Obeta-D-fructose + ?
show the reaction diagram		Arabidopsis thaliana--670539--?
levan-type fructans (beta-2,6) + H2OD-fructose
show the reaction diagram		Beta vulgaris-best substrate657006--?
levanbiose + H2Obeta-D-fructose + ?
show the reaction diagram		Triticum aestivum--669668--?
levanohexaose + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Aspergillus awamoriQ96TU3levanopentaose, levanotetraose, levanotriose, levanobiose and levan are intermediate products654875--?
levanopentaose + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Aspergillus awamoriQ96TU3levanotetraose, levanotriose, levanobiose and levan are intermediate products654875--?
levanotetraose + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Aspergillus awamoriQ96TU3levanotriose, levanobiose and levan are intermediate products654875--?
neokestin + H2OD-fructose
show the reaction diagram		Avena sativa--208823-208823?
neokestose + H2OD-fructose + sucrose
show the reaction diagram		Avena sativa--208823-208823?
neokestose + H2Obeta-D-fructose + sucrose
show the reaction diagram		Triticum aestivum-4% activity of isoform w1 and 3% of w2 in comparison to substrate 1-kestose657048--?
neokestose + H2O?
show the reaction diagram		Arctium lappa-7% activity compared to 1-kestose714715--?
neokestose + H2Obeta-D-fructose + ?
show the reaction diagram		Beta vulgaris, Arabidopsis thaliana, Cichorium intybus--670976--?
nystose + H2O?
show the reaction diagram		Arctium lappa-66% activity compared to 1-kestose714715--?
phlein + H2Obeta-D-fructose + ?
show the reaction diagram		Triticum aestivum--669668--?
raffinose + H2OD-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside
show the reaction diagram		Streptococcus mutans--208817, 208820--?
raffinose + H2OD-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside
show the reaction diagram		Streptococcus salivarius--208818-208818?
raffinose + H2OD-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside
show the reaction diagram		Actinomyces naeslundii--136519--?
raffinose + H2Obeta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
show the reaction diagram		Bacillus sp.--657429--?
raffinose + H2Obeta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
show the reaction diagram		Penicillium sp.Q8J0G118% of the activity toward inulin655019--?
raffinose + H2Obeta-D-fructofuranose + O6-alpha-D-galactopyranosyl-alpha-D-glucopyranoside
show the reaction diagram		Kluyveromyces marxianus-hydrolyzed at 69% of the activity with inulin681357--?
raffinose + H2Obeta-D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranose
show the reaction diagram		Alternaria alternata--655714--?
raffinose + H2Obeta-D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-glucopyranoside
show the reaction diagram		Aspergillus fumigatus--654218--?
stachyose + H2Obeta-D-fructose + alpha-D-galactopyranosyl-1,6-alpha-D-galactopyranosyl-1,6-alpha-D-galactopyranose
show the reaction diagram		Aspergillus fumigatus--654218--?
sucrose + H2Obeta-D-fructose + alpha-D-glucose
show the reaction diagram		Aspergillus fumigatus--654218--?
sucrose + H2Obeta-D-fructose + alpha-D-glucose
show the reaction diagram		Alternaria alternata--655714--?
sucrose + H2Obeta-D-fructose + alpha-D-glucose
show the reaction diagram		Triticum aestivum-100% activity716041--?
sucrose + H2O?
show the reaction diagram		Arctium lappa-0.2% activity compared to 1-kestose714715--?
sucrose + H2O?
show the reaction diagram		Phleum pratense-less than 1%, 2% and 15% activity towards 1 mM, 10 mM and 100 mM sucrose compared to levan715762--?
sucrose + H2OD-fructose + D-glucose
show the reaction diagram		Penicillium sp.--680950--?
sucrose + H2OD-fructose + D-glucose
show the reaction diagram		Streptococcus mutans--208817--?
sucrose + H2OD-fructose + D-glucose
show the reaction diagram		Streptococcus salivarius--208818-208818?
sucrose + H2OD-fructose + D-glucose
show the reaction diagram		Actinomyces naeslundii--136519--?
sucrose + H2OD-fructose + D-glucose
show the reaction diagram		Kluyveromyces marxianus-hydrolyzed at 177.6% of the activity with inulin681357--?
sucrose + H2Oalpha-D-glucose + beta-D-fructose
show the reaction diagram		Lactobacillus pentosus-3% enzyme activity in comparison to levan657381--?
sucrose + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Bacillus sp.--657429--?
sucrose + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Aspergillus ficuum--655298--?
sucrose + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Aspergillus awamoriQ96TU3-654875--?
sucrose + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Aspergillus nigerQ96WZ8-657274--?
sucrose + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Penicillium sp.Q8J0G1-655019--?
sucrose + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Kluyveromyces marxianus-highest activity714499--?
sucrose + H2Obeta-D-fructose + D-glucose
show the reaction diagram		Aspergillus awamori--669841--?
wheat graminan + H2Obeta-D-fructose + ?
show the reaction diagram		Triticum aestivum-high degree of polymerization668724--?
levanotriose + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Aspergillus awamoriQ96TU3levanobiose and levan are intermediate products654875--?
additional information?-Streptococcus salivarius-not: melizitose, dextran, pseudonigeran208818---
additional information?-Cichorium intybus-the enzyme catalyses the fructan breakdown in plants656993---
additional information?-Triticum aestivum-the enzyme might play a role as a beta-(2,1)-trimmer throughout the period of active graminan657048---
additional information?-Cichorium intybus-the enzyme is involved in fructan breakdown654116---
additional information?-Cichorium intybus-the enzyme is not able to break the alpha (1-2) linkage present in sucrose656993---
additional information?-Cichorium intybusQ93X60no activity towards sucrose660179---
additional information?-Triticum aestivum-no activity towards sucrose and high degree of polymerization-inulin668724---
additional information?-Triticum aestivum-no activity towards sucrose, 1-kestose, nystose, inulin669668---
additional information?-Lolium perenneQ64GB3no hydrolase activity towards sucrose and loliose681133---
additional information?-Arctium lappa-no activity towards levan, the enzyme preferentially hydrolyzes the beta-2,1 linkage of terminal fructosyl residue of fructan714715---
additional information?-Phleum pratense-the recombinant enzyme completely degrades fructans that are composed mainly of beta(2,6)-linked and linear fructans (levan) with a high degree of polymerization. The enzyme has significant activity against beta(2,1)-linked fructans, but considerably less than against beta(2,6)-linked fructans. The enzyme also has weak invertase activity715762---

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
fructans + H2OD-fructose
show the reaction diagram		Helianthus tuberosus-degradation208819--
fructans + H2OD-fructose
show the reaction diagram		Streptococcus mutans-degradation208817--
inulin + H2Ooligofructosides
show the reaction diagram		Alternaria alternata--655714--
inulin + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Aspergillus ficuum--655298--
inulin + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Aspergillus awamoriQ96TU3-654875--
inulin + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Aspergillus nigerQ96WZ8-657274--
inulin + H2Obeta-D-fructofuranose + alpha-D-glucopyranose
show the reaction diagram		Penicillium sp.Q8J0G1-655019--
additional information?-Cichorium intybus-the enzyme catalyses the fructan breakdown in plants656993--
additional information?-Triticum aestivum-the enzyme might play a role as a beta-(2,1)-trimmer throughout the period of active graminan657048--

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
No entries in this field

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
Ba2+Alternaria alternata-30 min at 30ºC, 10 mM, 140% of the relative activity without metal ions; 30 min at 30ºC, 1 mM, 118% of the relative activity without metal ions; 30 min at 30ºC, 5 mM, 132% of the relative activity without metal ions655714
Ca2+Alternaria alternata-30 min at 30ºC, 1 mM, 118% of the relative activity without metal ions; 30 min at 30ºC, 5 mM, 108% of the relative activity without metal ions655714
Ca2+Aspergillus nigerQ96WZ8at 55ºC, pH 4.5, 5 mM, 138% of the relative activity without metal ions657274
Ca2+Kluyveromyces marxianus-maximal increase in activity: 1.2fold681357
Ca2+Cryptococcus aureus-activating at concentrations of 1.0 mM690488
Ca2+Kluyveromyces marxianus-10% increase of activity at 20 mM Ca2+714499
Co2+Streptococcus mutans-slight stimulation208817
Co2+Penicillium sp.Q8J0G11.0 mM, pH 5.0, at 30ºC, 117% of the relative activity without any added compound655019
Cu2+Cryptococcus aureus-activating at concentrations of 1.0 mM690488
Fe2+Aspergillus nigerQ96WZ8at 55ºC, pH 4.5, 5 mM, 123% of the relative activity without metal ions657274
Fe2+Cryptococcus aureus-activating at concentrations of 1.0 mM690488
K+Cryptococcus aureus-activating at concentrations of 1.0 mM690488
Mg2+Alternaria alternata-30 min at 30ºC, 1 mM, 110% of the relative activity without metal ions655714
Mn2+Streptococcus mutans-slight stimulation208817
Mn2+Streptococcus salivarius-slight stimulation208818
Mn2+Penicillium sp.Q8J0G11.0 mM, pH 5.0, at 30ºC, 127% of the relative activity without any added compound655019
Mn2+Alternaria alternata-30 min at 30ºC, 1 mM, 109% of the relative activity without metal ions655714
Mn2+Aspergillus nigerQ96WZ8at 55ºC, pH 4.5, 5 mM, 112% of the relative activity without metal ions657274
Mn2+Kluyveromyces marxianus-maximal increase in activity: 2.4fold681357
Na+Cryptococcus aureus-activating at concentrations of 1.0 mM690488
Zn2+Cryptococcus aureus-activating690488

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
1,10-phenanthrolineCryptococcus aureus-strong inhibitor, after preincubation of the purified enzyme for 1 h at 0°C690488 2D-image
2,5 dideoxy-2,5-imino-D-mannitolCichorium intybus--682143 2D-image
Ag+Streptococcus mutans--208817 2D-image
Ag+Streptococcus salivarius--208818 2D-image
Ag+Penicillium sp.Q8J0G11.0 mM, pH 5.0, at 30ºC, 100% inhibition655019 2D-image
Ag+Kluyveromyces marxianus-potent inhibitor below 1 mM681357 2D-image
Ag+Cryptococcus aureus-decreased activity at concentrations of 1.0 mM690488 2D-image
Ag+Kluyveromyces marxianus-more than 50% of residual enzymatic activity at 20 mM714499 2D-image
Al3+Aspergillus nigerQ96WZ8at 55ºC, pH 4.5, 5 mM, 89% inhibition657274 2D-image
Ba2+Kluyveromyces marxianus-more than 50% of residual enzymatic activity at 20 mM714499 2D-image
CaCl2Cichorium intybus-partially208825 2D-image
Co2+Alternaria alternata-30 min at 30ºC, 10 mM, 70% inhibition; 30 min at 30ºC, 1 mM, 17% inhibition; 30 min at 30ºC, 5 mM, 39% inhibition655714 2D-image
Co2+Lactobacillus pentosus--657381 2D-image
Cu2+Streptococcus mutans-partially208817 2D-image
Cu2+Streptococcus salivarius--208818 2D-image
Cu2+Penicillium sp.Q8J0G11.0 mM, pH 5.0, at 30ºC, 26% inhibition655019 2D-image
Cu2+Alternaria alternata-30 min at 30ºC, 10 mM, 100% inhibition; 30 min at 30ºC, 1 mM, 23% inhibition; 30 min at 30ºC, 5 mM, 87% inhibition655714 2D-image
Cu2+Aspergillus nigerQ96WZ8at 55ºC, pH 4.5, 5 mM, 20% inhibition657274 2D-image
Cu2+Lactobacillus pentosus--657381 2D-image
Cu2+Kluyveromyces marxianus-less than 50% of residual enzymatic activity at 20 mM714499 2D-image
CuSO4Kluyveromyces marxianus-1 mM, 47% inhibition681357 2D-image
D-frucotseAspergillus ficuum-end product inhibitor, competitive, reversible690509-
D-fructoseCichorium intybus--682143 2D-image
D-glucoseAspergillus nigerQ96WZ8at 55ºC, pH 4.5, concentration higher than 2%657274 2D-image
D-glucoseAspergillus ficuum-competitive inhibitor690509 2D-image
EDTACichorium intybus-partially208825 2D-image
EDTAAspergillus fumigatus-1 mM, 63% of inhibition654218 2D-image
EDTAPenicillium sp.Q8J0G11.0 mM, pH 5.0, at 30ºC, 12% inhibition655019 2D-image
EDTAAlternaria alternata-30 min at 30ºC, 10 mM, 10% inhibition; 30 min at 30ºC, 5 mM, 9% inhibition655714 2D-image
EDTALactobacillus pentosus--657381 2D-image
EDTAKluyveromyces marxianus-1 mM, 50% inhibition681357 2D-image
EDTACryptococcus aureus-strong inhibitor, after preincubation of the purified enzyme for 1 h at 0°C690488 2D-image
EDTAKluyveromyces marxianus--714499 2D-image
Fe2(SO4)3Kluyveromyces marxianus-1 mM, 38% inhibition681357 2D-image
Fe2+Lactobacillus pentosus--657381 2D-image
Fe2+Kluyveromyces marxianus-more than 50% of residual enzymatic activity at 20 mM714499 2D-image
Fe3+Streptococcus salivarius--208818 2D-image
Fe3+Streptococcus mutans--208820 2D-image
Fe3+Penicillium sp.Q8J0G11.0 mM, pH 5.0, at 30ºC, 62% inhibition655019 2D-image
Fe3+Alternaria alternata-30 min at 30ºC, 10 mM, 86% inhibition; 30 min at 30ºC, 1 mM, 19% inhibition; 30 min at 30ºC, 5 mM, 60% inhibition655714 2D-image
Fe3+Aspergillus nigerQ96WZ8at 55ºC, pH 4.5, 5 mM, 45% inhibition657274 2D-image
Fe3+Kluyveromyces marxianus-more than 50% of residual enzymatic activity at 20 mM714499 2D-image
Hg2+Streptococcus mutans--208817, 208820 2D-image
Hg2+Streptococcus salivarius--208818 2D-image
Hg2+Aspergillus fumigatus-1 mM, 100% of inhibition654218 2D-image
Hg2+Penicillium sp.Q8J0G11.0 mM, pH 5.0, at 30ºC, 100% inhibition655019 2D-image
Hg2+Alternaria alternata-30 min at 30ºC, 10 mM, 85% inhibition; 30 min at 30ºC, 1 mM, 26% inhibition; 30 min at 30ºC, 5 mM, 57% inhibition655714 2D-image
Hg2+Aspergillus nigerQ96WZ8at 55ºC, pH 4.5, 5 mM, 91% inhibition657274 2D-image
Hg2+Kluyveromyces marxianus-potent inhibitor below 1mM681357 2D-image
Hg2+Cryptococcus aureus-decreased activity at concentrations of 1.0 mM690488 2D-image
Hg2+Kluyveromyces marxianus-less than 50% of residual enzymatic activity at 20 mM714499 2D-image
inulinCryptococcus aureus--690488 2D-image
iodoacetateAlternaria alternata-30 min at 30ºC, 10 mM, 87% inhibition; 30 min at 30ºC, 1 mM, 38% inhibition; 30 min at 30ºC, 5 mM, 57% inhibition655714 2D-image
iodoacetic acidCryptococcus aureus-strong inhibitor, after preincubation of the purified enzyme for 1 h at 0°C690488 2D-image
Mg2+Aspergillus nigerQ96WZ8at 55ºC, pH 4.5, 5 mM, 41% inhibition657274 2D-image
Mg2+Cryptococcus aureus-decreased activity at concentrations of 1.0 mM690488 2D-image
Mn2+Alternaria alternata-30 min at 30ºC, 10 mM, 20% inhibition655714 2D-image
N-bromosuccinimidePenicillium sp.Q8J0G11.0 mM, pH 5.0, at 30ºC, 100% inhibition655019 2D-image
p-chloromercuribenzoateStreptococcus salivarius-presence of cysteine protects against inactivation but no recovery208818 2D-image
p-chloromercuribenzoatePenicillium sp.Q8J0G11.0 mM, pH 5.0, at 30ºC, 65% inhibition655019 2D-image
p-chloromercuribenzoateLactobacillus pentosus--657381 2D-image
phenylmethanesulfonyl fluorideCryptococcus aureus-strong inhibitor, after preincubation of the purified enzyme for 1 h at 0°C690488 2D-image
SDSCryptococcus aureus-final concentration of 10.0 mM690488 2D-image
Sodium arsenateAlternaria alternata-30 min at 30ºC, 10 mM, 50% inhibition; 30 min at 30ºC, 1 mM, 20% inhibition; 30 min at 30ºC, 5 mM, 38% inhibition655714 2D-image
Sodium arseniteAlternaria alternata-30 min at 30ºC, 10 mM, 58% inhibition; 30 min at 30ºC, 1 mM, 28% inhibition; 30 min at 30ºC, 5 mM, 41% inhibition655714 2D-image
SucroseCichorium intybus--208825, 670976 2D-image
SucroseArabidopsis thaliana--670976, 682426 2D-image
SucroseBeta vulgaris--670976 2D-image
SucroseLolium perenneQ64GB390% inhibition at 20 mM681133 2D-image
SucroseCichorium intybus-; 63% inhibition by 10 mM sucrose, wild-type enzyme. Mutant enzymes W82L and S101L are not inhibited by sucrose. W82 and S101 are important for binding sucrose as inhibitor682143 2D-image
SucrosePhleum pratense-the inhibitory effect of sucrose is 4% at 10 mM sucrose and 23% at 100 mM sucrose715762 2D-image
TrisStreptococcus salivarius--208818 2D-image
Zn2+Streptococcus mutans--208820 2D-image
Zn2+Alternaria alternata-30 min at 30ºC, 10 mM, 66% inhibition; 30 min at 30ºC, 1 mM, 22% inhibition; 30 min at 30ºC, 5 mM, 39% inhibition655714 2D-image
Zn2+Aspergillus nigerQ96WZ8at 55ºC, pH 4.5, 5 mM, 8% inhibition657274 2D-image
Zn2+Lactobacillus pentosus--657381 2D-image
Zn2+Kluyveromyces marxianus-less than 50% of residual enzymatic activity at 20 mM714499 2D-image
Mn2+Kluyveromyces marxianus-less than 50% of residual enzymatic activity at 20 mM714499 2D-image
additional informationAspergillus nigerQ96WZ8no inhibited by EDTA and 2-mercaptoethanol657274-
additional informationTriticum aestivum-not inhibited by sucrose669668-
additional informationAspergillus niger-fructanohydrolase biosynthesis in the basal medium is reduced drastically with the addition of actinomycin D679966-
additional informationLolium perenneQ64GB3glucose and fructose (20 mM) do not affect 1-FEHa activity681133-
additional informationCichorium intybus-glycosylation of 1-FEH IIa affected overall enzyme activity682145-

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
DDTAspergillus nigerQ96WZ8at 55ºC, pH 4.5, 5 mM, 121% of the relative activity without metal ions657274 2D-image
EDTAStreptococcus salivarius-slight stimulatuion after 30 min of incubation208818 2D-image
KCNStreptococcus salivarius-slight stimulatuion after 30 min of incubation208818 2D-image
additional informationTriticum aestivum-water stress activates660316-
additional informationAspergillus niger-the extracellular enzyme activity increases when sucrose ester S-770 is added to the basal medium679966-

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.6-1-kestoseArabidopsis thalianaQ67XZ3in 50 mM sodium acetate buffer, pH 5.0, at 30°C682426 2D-image
7-1-kestoseTriticum aestivum-pH 5.0, 30°C657048 2D-image
27.8-ActilightKluyveromyces marxianus-purified enzyme, at pH 5.0 and 55°C714499-
12.9-Agave tequilana fructanKluyveromyces marxianus-purified enzyme, at pH 5.0 and 50°C714499-
0.05-inulinAspergillus awamoriQ96TU3at 37ºC, pH 4.5654875 2D-image
0.0926-inulinPenicillium sp.Q8J0G1at 40ºC, pH 5.0655019 2D-image
0.22-inulinPenicillium sp.-InuE:D' hybrid680950 2D-image
0.25-inulinAspergillus fumigatus--654218 2D-image
2.57-inulinAspergillus nigerQ96WZ8at 55ºC, pH 4.5657274 2D-image
3-inulinPenicillium sp.-InuE680950 2D-image
3.4-inulinKluyveromyces marxianus-pH 5.5, 50°C681357 2D-image
4.75-inulinAspergillus ficuum-incubation at 50°C, 1 h690509 2D-image
7.16-inulinKluyveromyces marxianus-purified enzyme, at pH 5.0 and 55°C714499 2D-image
66-inulinAlternaria alternata-at 55ºC, pH 4.6655714 2D-image
3.72-InulobioseAspergillus awamoriQ96TU3at 37ºC, pH 4.5654875 2D-image
1.1-inuloheptaoseAspergillus awamoriQ96TU3at 37ºC, pH 4.5654875 2D-image
1.02-inulohexaoseAspergillus awamoriQ96TU3at 37ºC, pH 4.5654875 2D-image
1.08-inulopentaoseAspergillus awamoriQ96TU3at 37ºC, pH 4.5654875 2D-image
0.94-inulotetraoseAspergillus awamoriQ96TU3at 37ºC, pH 4.5654875 2D-image
1.63-inulotrioseAspergillus awamoriQ96TU3at 37ºC, pH 4.5654875 2D-image
1.8-levanKluyveromyces marxianus-purified enzyme, at pH 5.0 and 50°C714499 2D-image
1.01-levanohexaoseAspergillus awamoriQ96TU3at 37ºC, pH 4.5654875 2D-image
10.2-levanopentaoseAspergillus awamoriQ96TU3at 37ºC, pH 4.5654875 2D-image
2.74-levanotetraoseAspergillus awamoriQ96TU3at 37ºC, pH 4.5654875 2D-image
2.7-raffinoseKluyveromyces marxianus-pH 5.5, 50°C681357 2D-image
25.3-SucroseKluyveromyces marxianus-pH 5.5, 50°C681357 2D-image
29.1-SucroseKluyveromyces marxianus-purified enzyme, at pH 5.0 and 55°C714499 2D-image
40-SucroseAspergillus awamoriQ96TU3at 37ºC, pH 4.5654875 2D-image
48.7-SucrosePenicillium sp.-InuE:D' hybrid680950 2D-image
111-SucrosePenicillium sp.-InuE680950 2D-image
0.21-levanotrioseAspergillus awamoriQ96TU3at 37ºC, pH 4.5654875 2D-image
additional information-additional informationAspergillus awamoriQ96TU3Michaelis-Menten kinetics654875-
additional information-additional informationAspergillus nigerQ96WZ8Michaelis-Menten kinetics657274-
additional information-additional informationCryptococcus aureus-20.06 mg/ml for inulin690488-

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
21-1-kestoseArabidopsis thalianaQ67XZ3in 50 mM sodium acetate buffer, pH 5.0, at 30°C682426 2D-image
1.09-ActilightKluyveromyces marxianus-purified enzyme, at pH 5.0 and 55°C714499-
0.9-Agave tequilana fructanKluyveromyces marxianus-purified enzyme, at pH 5.0 and 50°C714499-
0.1-inulinKluyveromyces marxianus-purified enzyme, at pH 5.0 and 55°C714499 2D-image
500-inulinPenicillium sp.-InuE680950 2D-image
1110-inulinPenicillium sp.-InuE:D' hybrid680950 2D-image
0.33-levanKluyveromyces marxianus-purified enzyme, at pH 5.0 and 50°C714499 2D-image
1.27-SucroseKluyveromyces marxianus-purified enzyme, at pH 5.0 and 55°C714499 2D-image
3330-SucrosePenicillium sp.-InuE:D' hybrid680950 2D-image
5000-SucrosePenicillium sp.-InuE680950 2D-image

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
1.09-ActilightKluyveromyces marxianus-purified enzyme, at pH 5.0 and 55°C7144990
0.9-Agave tequilana fructanKluyveromyces marxianus-purified enzyme, at pH 5.0 and 50°C7144990
0.1-inulinKluyveromyces marxianus-purified enzyme, at pH 5.0 and 55°C71449911722
0.18-levanKluyveromyces marxianus-purified enzyme, at pH 5.0 and 50°C71449912614
1.27-SucroseKluyveromyces marxianus-purified enzyme, at pH 5.0 and 55°C71449916818

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
3.5-SucroseCichorium intybus--682143 2D-image

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
0.2-Cryptococcus aureus-reaction mixture consists of 0.1 ml purified inulinase, 0.9 ml acetate buffer, 20 g/l inulin; incubation at 50°C for 10 min; reaction inactivated at 100°C for 10 min690488
1.765-Arctium lappa-enzyme from supernatant, at pH 6.5 and 30°C714715
4.02-Kluyveromyces marxianus-crude extract, at pH 5.0 and 50°C714499
12.2-Triticum aestivum-isoform 1-FEH w1657048
13.65-Triticum aestivum-native protein, pH 5.0669668
14.6-Triticum aestivum-isoform 1-FEH w1657048
26.6-Triticum aestivum-protein expressed in Pichia pastoris, pH 5.0669668
40.4-Penicillium sp.-substrate: inulin, recombinant InuE680950
49.5-Kluyveromyces marxianus-after 12.3fold purification, at pH 5.0 and 50°C714499
73.4-Arctium lappa-after 41.6fold purification, at pH 6.5 and 30°C714715
93.8-Penicillium sp.Q8J0G1sucrose, at 40ºC, pH 5.0655019
150-Penicillium sp.-substrate: sucrose, recombinant InuE680950
156-Aspergillus nigerQ96WZ8at 55ºC, pH 4.5657274
171-Penicillium sp.-substrate: sucrose, InuE:D' hybrid680950
261-Lactobacillus pentosus--657381
280-Penicillium sp.-substrate: inulin,InuE:D' hybrid680950
413-Kluyveromyces marxianus--681357
743-Penicillium sp.Q8J0G1inulin, at 40ºC, pH 5.0655019
12300-Aspergillus ficuum-pH 5.0, 50°C690509
80700-Alternaria alternata--655714
additional information-Aspergillus fumigatus-171 U/mg of protein654218
additional information-Bacillus sp.-19.0 ?mol/min/ml, for sucrose, pH 7, at 60ºC; 2.0 ?mol/min/ml, for inulin, pH 7, at 60ºC; 6.2 ?mol/min/ml, for raffinose, pH 7, at 60ºC657429

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
45.5Penicillium janczewskii--682058
46.5Kluyveromyces marxianus-for Agave tequilana fructan as substrate, the enzymatic activity is constant from pH 4.0 to 6.5714499
4-Penicillium sp.Q8J0G1at 40ºC655019
4-Penicillium sp.-substrate: inulin or sucrose, recombinant enzyme InuE680950
4.55Kluyveromyces marxianus-using levan as substrate714499
4.55.5Avena sativa-neokestin208823
4.55.5Triticum aestivum-both isoforms w1 and w2657048
4.5-Streptococcus mutans-sucrose208817
4.5-Alternaria alternata-at 55ºC, enzyme retains only 90% of its activity655714
4.5-Aspergillus nigerQ96WZ8at 55ºC657274
4.5-Penicillium sp.-substrate: inulin and sucrose, InuE:D' hybrid680950
55.5Cichorium intybus-inulin208825
5-Streptococcus mutans-sucrose208820
5-Cryptococcus aureus, Debaryomyces hansenii--681774
5-Cryptococcus aureus--690488
5-Kluyveromyces marxianus-using inulin, Actilight and sucrose as substrates714499
5.4-Aspergillus ficuum--690509
5.5-Streptococcus mutans-fructan polymers208817
5.5-Streptococcus mutans-levan208820
5.5-Aspergillus fumigatus--654218
5.5-Lactobacillus pentosus--657381
5.5-Lolium perenneQ64GB3-681133
5.5-Kluyveromyces marxianus--681357
5.5-Phleum pratense--715762
5.8-Arctium lappa--714715
6-Streptococcus mutans-linulin208820
6-Meyerozyma guilliermondii-crude inulinase681217
6-Meyerozyma guilliermondii--681774
6.57Bacillus sp.-at pH 8.0, 58% of maximal activity657429
7-Streptococcus salivarius--208818

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
3.57Streptococcus mutans-little fructanase activity at or above pH 7.0208817
46Lactobacillus pentosus-85% of maximal activity at both pH 4.0 and 6.0657381
46.5Cryptococcus aureus-above and below rapid decrease of enzyme activity690488
47Cryptococcus aureus-pH 4.0: about 20% of maximal activity, pH 7.0: about 45% of maximal activity681774
47Debaryomyces hansenii-pH 4.0: about 40% of maximal activity, pH 7.0: about 45% of maximal activity681774
4.58Streptococcus salivarius--208818
56.2Aspergillus ficuum--690509
57Meyerozyma guilliermondii-pH 5: about 30% of maximal activity, pH 7.0: about 50% of maximal activity, crude inulinase681217
57Meyerozyma guilliermondii-pH 5.0: about 30% of maximal activity, pH 7.0: about 55% of maximal activity681774

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
25-Lactobacillus pentosus--657381
3040Triticum aestivum-both isoforms w1 and w2657048
30-Phleum pratense--715762
35-Cichorium intybus-inulin208825
50-Streptococcus salivarius--208818
50-Kluyveromyces marxianus--681357
50-Cryptococcus aureus, Debaryomyces hansenii--681774
50-Cryptococcus aureus--690488
50-Aspergillus ficuum--690509
50-Kluyveromyces marxianus-the optimal temperature for levan and Agave tequilana fructan hydrolysis is 50°C714499
55-Streptococcus mutans-levan208820
55-Penicillium sp.Q8J0G1pH 5.0655019
55-Alternaria alternata-pH 4.6655714
55-Aspergillus nigerQ96WZ8pH 5.5657274
55-Penicillium sp.-substrate: inulin, InuE:D' hybrid680950
55-Kluyveromyces marxianus-the optimal temperature for inulin, Actilight and sucrose hydrolysis is 55°C714499
60-Aspergillus fumigatus-enzyme retains 35.9% and 25.8% activities after 4 h at 50 and 55ºC, respectively654218
60-Penicillium sp.-substrate: inulin or sucrose, recombinant enzyme InuE; substrate: sucrose, InuE:D' hybrid680950
60-Meyerozyma guilliermondii-crude inulinase681217
60-Meyerozyma guilliermondii--681774
60-Penicillium janczewskii--682058
65-Bacillus sp.--657429

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
1535Lactobacillus pentosus-65% of maximal activity at 15°C, 70% of maximal activity at 35°C657381
2055Streptococcus salivarius-rapid inactivation at 60°C208818
3050Aspergillus ficuum--690509
3570Cryptococcus aureus--690488
4060Streptococcus mutans--208820
4065Cryptococcus aureus-40°C: about 65% of maximal activity, 65°C: about 55% of maximal activity681774
4065Debaryomyces hansenii-40°C: about 55% of maximal activity, 65°C: about 65% of maximal activity681774
4070Meyerozyma guilliermondii-40°C: about 70% of maximal activity 70°C: about 70% of maximal activity, crude inulinase681217
4070Meyerozyma guilliermondii-40°C: about 70% of maximal activity, 70°C: about 70% of maximal activity681774

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
4.64.7Aspergillus fumigatus-isoelectric focusing654218
4.9-Penicillium sp.Q8J0G1analytical isoelectric focusing; deduction from sequence of amino acid655019
4.9-Cichorium intybus-both isoforms657033
4.9-Arctium lappa-calculated from amino acid sequence714715
5-Phleum pratense-calculated from amino acid sequence715762
5.2-Triticum aestivum--668724
5.2-Lolium perenneQ64GB3isoelectric focusing681133
6.5-Cichorium intybus--656993
additional information-Beta vulgaris--657006

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
culture filtratePenicillium sp.Q8J0G1-655019Manually annotated by BRENDA team
culture filtrateAlternaria alternata--655714Manually annotated by BRENDA team
culture filtratePenicillium janczewskii--682058Manually annotated by BRENDA team
culture mediumAspergillus awamoriQ96TU3-654875Manually annotated by BRENDA team
culture mediumKluyveromyces marxianus--678752Manually annotated by BRENDA team
culture supernatantBacillus sp.--657429Manually annotated by BRENDA team
culture supernatantKluyveromyces marxianus-optimization of medium and process parameters for the production of inulinase678752Manually annotated by BRENDA team
culture supernatantMeyerozyma guilliermondii-the optimal medium for inulinase production is seawater containing 4.0% (w/v) inulin and 0.5% (w/v) yeast extract, while the optimal cultivation conditions for inulinase production were pH 8.0, 28°C and 170 rpm681217Manually annotated by BRENDA team
epicotylTriticum aestivum--668724Manually annotated by BRENDA team
leafCichorium intybus--657033Manually annotated by BRENDA team
leafLolium perenneQ64GB3-681133Manually annotated by BRENDA team
leafTriticum aestivum-enzyme expression analysis of isozymes under different water conditions, overview705947Manually annotated by BRENDA team
myceliumPenicillium sp.Q8J0G1transcription level of inuD increases 3260fold in inulin-grown mycelia. Glucose exerts a strong carbon catabolite repression on the expression of inuD680946Manually annotated by BRENDA team
plant crownPhleum pratense--715762Manually annotated by BRENDA team
rhizophoreVernonia herbaceaA9JIF31-FEH activity is high only in induced sprouting; spatial and temporal distribution, mainly in the distal region of the rhizophore at vegetative phase and in the proximal region at sprouting phase670618Manually annotated by BRENDA team
rootCichorium intybus-in field-grown roots throughout autumn, highly expression in cold-induced roots and only low expression in forced roots656993Manually annotated by BRENDA team
rootBeta vulgaris--657006Manually annotated by BRENDA team
rootCichorium intybus-forced roots657033Manually annotated by BRENDA team
rootSmallanthus sonchifolius--681161Manually annotated by BRENDA team
stemTriticum aestivum-comparison of activities during stem development657048Manually annotated by BRENDA team
tuberHelianthus tuberosus--208819Manually annotated by BRENDA team
leafTriticum aestivum--716041Manually annotated by BRENDA team
additional informationTriticum aestivum-no activity in leaves668724Manually annotated by BRENDA team

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
extracellularAspergillus fumigatus---654218Manually annotated by BRENDA team
extracellularAspergillus awamori---654875, 669841Manually annotated by BRENDA team
extracellularPenicillium sp.---655019, 680950Manually annotated by BRENDA team
extracellularAlternaria alternata---655714Manually annotated by BRENDA team
extracellularBacillus sp.---657429Manually annotated by BRENDA team
extracellularKluyveromyces marxianus---678752, 678762, 681357Manually annotated by BRENDA team
extracellularPenicillium janczewskii---682058Manually annotated by BRENDA team
vacuoleHelianthus tuberosus--5773208819Manually annotated by BRENDA team
vacuoleHordeum vulgare--5773208824Manually annotated by BRENDA team
extracellularCryptococcus aureus-fermented broth-690488Manually annotated by BRENDA team
additional informationCichorium intybus-the enzyme is unexpectedly related to cell-wall invertases, although all other data suggest a vacuolar localization-656993Manually annotated by BRENDA team
additional informationCichorium intybus-the enzyme is unexpectedly related to cell-wall invertases, although a vacuolar localization is proposed-657033Manually annotated by BRENDA team

PDBSCOPCATHORGANISM
4azz, downloadSCOP (4azz)CATH (4azz)Bacillus subtilis (strain 168)
4b1l, downloadSCOP (4b1l)CATH (4b1l)Bacillus subtilis (strain 168)
4b1m, downloadSCOP (4b1m)CATH (4b1m)Bacillus subtilis (strain 168)

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
60000-Cichorium intybus-gel-filtration208825
60000-Cryptococcus aureus-after purification, analyzed by SDS-PAGE690488
63000-Aspergillus ficuum-SDS-PAGE690509
64000-Cichorium intybus-SDS-PAGE208825
70000-Triticum aestivum-SDS-PAGE669668
74520-Penicillium sp.Q8J0G1calculation from sequence of amino acid655019
80000-Penicillium janczewskii-gel filtration, non-denaturing PAGE682058
81000-Penicillium sp.Q8J0G1SDS-PAGE655019
8300085000Streptococcus salivarius-gel filtration, SDS-PAGE208818
83000-Aspergillus nigerQ96WZ8SDS-PAGE657274
115000-Alternaria alternata-gel filtration655714
126000-Lactobacillus pentosus-gel filtration657381
150000200000Bacillus subtilisP05656gel filtration714493
176500-Aspergillus fumigatus-native PAGE654218
200000-Aspergillus fumigatus-gel filtration654218
250000-Kluyveromyces marxianus-gel filtration714499

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
?Streptococcus mutans-x * 140000, SDS-PAGE208817
?Streptococcus mutans-x * 127000, SDS-PAGE208820
?Avena sativa-x * 43000, SDS-PAGE208823
?Hordeum vulgare-x * 33000, SDS-PAGE208824
?Penicillium sp.-x * 110000, InuE:D' hybrid, SDS-PAGE; x * 73924, InuE:D' hybrid, calculated from sequence680950
?Arctium lappa-x * 65666, calculated from amino acid sequence; x * 80000, SDS-PAGE714715
?Phleum pratense-x * 63400, calculated from amino acid sequence; x * 78000, SDS-PAGE715762
heterotrimerAspergillus fumigatus-1 * 66000 + 1 * 62700 + 1 * 59400, SDS-PAGE654218
homodimerKluyveromyces marxianus-2 * 125000, SDS-PAGE714499
monomerCichorium intybus-1 * 64000, SDS-PAGE657033
monomerTriticum aestivum-1 * 70000, SDS-PAGE657048
monomerLactobacillus pentosus-1 * 125000, SDS-PAGE657381
monomerPenicillium janczewskii-1 * 80000, SDS-PAGE682058

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
no modificationAlternaria alternata--655714
glycoproteinAspergillus awamori--669841
no modificationAspergillus awamoriQ96TU3-654875
glycoproteinAspergillus ficuum--655298
no modificationAspergillus nigerQ96WZ8-657274
no modificationBacillus sp.--657429
glycoproteinBacillus subtilisP05656hyper-glycosylated protein714493
glycoproteinCichorium intybusQ93X60-660179
glycoproteinCichorium intybus-glycosylation of 1-FEH IIa affects overall enzyme activity682145
glycoproteinKluyveromyces marxianus--714499
no modificationPenicillium sp.Q8J0G1-655019

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
enzyme-fructose-complex, 1.87 A resolution, single isomorphous replacement, 20% fructose solution; free enzyme, 1.5 A resolution, single isomorphous replacement, orthorhombic crystals, 0.5 M sodium iodide and flash-cooling to -173°C in stream of cold nitrogenAspergillus awamori-669841
2.35 A resolution, hanging drop vapour diffusion, structure determination by single anomalous dispersion methodCichorium intybusQ93X60660179
hanging-drop vapour diffusion method, crystallization of complexes between 1-FEH IIa with the substrate 1-kestose and the inhibitors sucrose, fructose and 2,5-dideoxy-2,5-imino-D-mannitol; mutant proteins are crystallized by hanging drop vapour diffusion method with 2.1 M sodium potassium phosphate and 0.1 M potassium phosphate, pH 7.0Cichorium intybus-682143
X-ray diffraction studyCichorium intybus-654116

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
3.5-Lactobacillus pentosus-55% of maximal activity657381
45Alternaria alternata-at 55ºC655714
46.5Cryptococcus aureus-rapid decrease of residual activity above and below, after treatment at pH 6.0 and 4°C for 2 h still 96.0% residual activity690488
49.5Aspergillus fumigatus--654218
4.510Penicillium sp.-30°C, 24 h, InuE:D', stable680950
4.5-Streptococcus salivarius-almost complete loss of activity at208818
56Kluyveromyces marxianus-50°C, 3 h, stable681357
57Penicillium sp.Q8J0G1at 30ºC655019
5.4-Aspergillus ficuum-stable for 60 min without decrease in activity, stable for 12 h with 96% residual activity, stable for 72 h with 63.8% residual activity690509
5.57.5Bacillus sp.-95-100% of maximal activity retained657429
5.59Arctium lappa--714715
7-Streptococcus salivarius-37°C, 30 min208818
7.5-Triticum aestivum-above pH 7.5 the isoforms are inactive657048

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
450Lolium perenneQ64GB320% of the maximal activity is still detected at 4°C, 1-FEHa activity decreases strongly above 40°C and falls to 20% of ist maximal activity at 50°C681133
4-Lactobacillus pentosus-more than 70% of maximal activity at pH 5.8 after 24 h657381
4-Phleum pratense-the activity of the enzyme remains relatively high even at 4°C, with up to 38% of its activity at 30°C715762
15-Lactobacillus pentosus-more than 70% of maximal activity at pH 5.8 after 24 h657381
25-Lactobacillus pentosus-more than 70% of maximal activity at pH 5.8 after 24 h657381
37-Streptococcus salivarius-pH 7.0, 30 min208818
37-Lactobacillus pentosus-more than 70% of maximal activity at pH 5.8 after 24 h657381
40-Kluyveromyces marxianus-the enzyme shows 100%, 84.2%, 98.6%, 92.9% and 100% residual activity after 72 h at 40°C when using levan, inulin, Agave tequilana ructan, Actilight and sucrose as substrates, respectively714499
40-Arctium lappa-stable at temperatures up to 40°C714715
45-Penicillium sp.-30 min, inulinase activity of InuE:D' is stable below680950
5070Penicillium sp.Q8J0G1pH 5.0, enzyme remains stable up to 50ºC, but it loses activity at 70ºC655019
50-Alternaria alternata-after 50ºC there is a gradual loss in activity, pH 4.6655714
50-Kluyveromyces marxianus-3 h, stable681357
50-Aspergillus ficuum-stable for 60 min without decrease in activity, stable for 12 h with 96% residual activity, stable for 72 h with 63.8% residual activity690509
60-Streptococcus salivarius-rapid inactivation208818
60-Bacillus sp.-without inulin, 73% of maximal activity after heating for 60 min; without inulin, 78% of maximal activity after heating for 15 min657429
60-Kluyveromyces marxianus-3 h, 50% loss of activity681357
65-Bacillus sp.-with inulin, 92% of maximal activity after heating for 60 min; with inulin, 97% of maximal activity after heating for 15 min; without inulin, 17% of maximal activity after heating for 60 min; without inulin, 34% of maximal activity after heating for 15 min657429
65-Cryptococcus aureus-above rapidly inactivating, after treatment at 65°C for 2 h still 86.0% residual activity, after treatment of 70°C for 2 h still 60.0% residual activity690488
70-Bacillus sp.-with inulin, 56% of maximal activity after heating for 60 min; with inulin, 85% of maximal activity after heating for 15 min; without inulin, 2% of maximal activity after heating for 60 min; without inulin, 7% of maximal activity after heating for 15 min657429
70-Kluyveromyces marxianus-3 h, 85% loss of activity681357

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
freezing and thawing accelerates inactivationStreptococcus salivarius-208818

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
10°C, 10 weeks, triggered by decrease in sucrose contentAllium cepa-665885
20°C, 10 weeks, triggered by decrease in sucrose contentAllium cepa-665885
at either -15ºC or at 4ºC, 200 mM acetate buffer, pH 4.6, 2 weeks, 50% loss of activityAlternaria alternata-655714
50°C, pH 5.4, stable for 60 min without decrease in activity, after 12 h only 96% residual activity, after 72 h only 63.8% residual activityAspergillus ficuum-690509
65°C, 2 h, 86% residual activity after treatment at 65°C, pH below 4.0 and above 6.5 results in rapid decrease in enzyme activityCryptococcus aureus-690488
4°C, 0.02 mg/ml, 20 mM phosphate buffer, pH 7.0, stable at least for several days, 90% loss of activity after 2 weeksStreptococcus salivarius-208818
higher stability at -20°C than at 4°CStreptococcus salivarius-208818

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
by ammonium sulfate precipitation, Sephadex column and DEAE-cellulose column, 62foldAlternaria alternata-655714
DEAE-Sepharose CL-6B column chromatography, Toyopearl HW55S column chromatography, and Toyopearl HW55S column chromatographyArctium lappa-714715
-Aspergillus awamori-669841
4.2-fold purification, 21% yieldAspergillus ficuum-690509
by ammonium sulfate precipitation, DEAE-Sephacel column, Sephacryl S-200 column, concanavalin A-linked amino-activated silica column and Sepharose 6B column, 25foldAspergillus fumigatus-654218
recombinant enzyme, by ammonium sulfate precipitation and anion exchange column, 4.8foldAspergillus nigerQ96WZ8657274
-Avena sativa-208823
ultrafiltration, 1.3foldBacillus sp.-657429
-Beta vulgaris-657006, 670976
-Cichorium intybus-208825, 656993, 660179, 682143, 682145
two independent isoforms 1-FEH IIa and 1-FEH IIb from leaves and rootsCichorium intybus-657033
7.2-fold purification, analyzed by SDS-PAGE, 2.44-fold increase of specific inulinase activity with 22.4% yield after DEAE sepharose fast flow anion exchange chromatographyCryptococcus aureus-690488
-Helianthus tuberosus-208819
-Hordeum vulgare-208824
partialKluyveromyces marxianus-681357
ultrafiltration and Mono Q Sepharose column chromatographyKluyveromyces marxianus-714499
Sephadex G50 gel filtrationLolium perenneQ64GB3681133
-Penicillium janczewskii-682058
by ultrafiltration, DEAE-cellulofine column, Q-Sepharose column and Superdex column, 329foldPenicillium sp.Q8J0G1655019
purification of recombinant inuE and inuE:D' fusion protein. The internal region D' encoding the 157-amino-acid sequence in the Penicillium exoinulinase gene inuD cDNA is inserted into the site between the nucleotides 897 and 898 of the Aspergillus niger exoinulinase gene inuE cDNA. The resultant inuE:D' fusion is expressed in Pichia pastoris and purifiedPenicillium sp.-680950
-Streptococcus mutans-208817, 208820
-Streptococcus salivarius-208818
ammonium sulfate precipitation, ConA affinity-, ion exchange-chromatography, and gel filtrationTriticum aestivum-669668
centrifugation, concentration and desaltation with 20 mM citrate-phosphate buffer pH 5.0 on Amicon Ultra-15 concentratorTriticum aestivum-668724
Sephadex G-25Triticum aestivum-660316
two isoforms 1-FEH w1 and w2Triticum aestivum-657048

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
expression in Escherichia coliActinomyces naeslundii-136519
expressed in Pichia pastorisArabidopsis thalianaQ67XZ3682426
heterologous expression in Pichia pastorisArabidopsis thaliana-670539
expressed in Pichia pastorisArctium lappa-714715
expression in Pichia pastorisAspergillus nigerQ96WZ8657274
a modified Bacillus subtilis levanase gene (sacC) in which the native bacterial signal sequence is replaced with a yeast alpha-factor domain is expressed in Saccharomyces cerevisiae strain AH22Bacillus subtilisP05656714493
expression in Pichia pastorisBeta vulgaris-657006
heterologous expression in Pichia pastorisBeta vulgaris-670976
-Cichorium intybus-682147
expressed in Escherichia coli Top10 cellsCichorium intybus-682143
expressed in Pichia pastoris; expression in Pichia pastorisCichorium intybus-682145
expression in potato tubersCichorium intybus-656993
heterologous expression in Pichia pastorisCichorium intybusQ93X60660179
the enzyme gene promoter is highly expressed in etiolated leaves and cold-stored rootsCichorium intybusQ93X60656406
expressed in Pichia pastoris strain X33Lolium perenneQ64GB3681133
in Escherichia coliPenicillium sp.Q8J0G1655019
the internal region D' encoding the 157-amino-acid sequence in the Penicillium exoinulinase gene inuD cDNA is inserted into the site between the nucleotides 897 and 898 of the Aspergillus niger exoinulinase gene inuE cDNA. The resultant inuE:D' fusion is expressed in Pichia pastorisPenicillium sp.-680950
expressed in Pichia pastorisPhleum pratense-715762
expression in Escherichia coliStreptococcus mutans-208817, 208821
-Triticum aestivum-657048
enzyme expression analysis of isozymes under different water conditions, overviewTriticum aestivum-705947
heterologous expression in Pichia pastorisTriticum aestivum-669668
heterologous expression in Pichia pastoris X-33Triticum aestivum-668724

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
in the stubble of seedlings and in young haplocorms from adult timothy plants, enzyme transcripts are significantly increased within 3 h of defoliationPhleum pratense-715762

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
E201QCichorium intybus-inactive682143
P294NCichorium intybus-mutant with an introduced N-glycosylation site near the inulin-binding cleft, shows highly decreased activity against higher DP inulin. The introduction of a glycosyl chain most probably blocks the cleft and prevents inulin binding and degradation; shows highly decreased activity against inulin682145
S101LCichorium intybus-inactive; no inhibition by sucrose682143
S101LCichorium intybus-strongly increased sucrose-hydrolysing activity compared with the wild-type enzym at high sucrose concentrations. At a very low substrate concentration (5 mm), the introduced sucrose hydrolysing activity is small compared with the activities towards the preferential 1-FEH IIa substrates 1-kestose and inulin682147
additional informationArabidopsis thalianaQ67XZ3a D239A mutant of cell wall invertase 1 acts as a 1-FEH, preferentially degrading 1-kestose682426
W82LCichorium intybus-inactive; no inhibition by sucrose682143
additional informationPenicillium sp.-the internal region D' encoding the 157-amino-acid sequence in the Penicillium exoinulinase gene inuD cDNA is inserted into the site between the nucleotides 897 and 898 of the Aspergillus niger exoinulinase gene inuE cDNA. The resultant inuE:D' fusion is expressed in Pichia pastoris. The Km-value of the secreted hybrid enzyme InuE:D' for inulin hydrolysis is about 1/15 that of the Aspergillus niger InuE, whereas its kcat value does not differ greatly from that of the InuE680950

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
synthesisAlternaria alternata-microbial production of high fructose syrup, alcohol, acetone and butanol655714
nutritionAspergillus awamoriQ96TU3microbial production of pure beta-D-fructofuranose or fructose syrup654875
food industryAspergillus ficuum-diabetics690509
nutritionAspergillus nigerQ96WZ8production of ultra high fructose syrup657274
nutritionBacillus sp.-microbial production of ultra high fructose glucose syrups657429
synthesisBacillus subtilisP05656expression of bacterial levanase in yeast enables simultaneous saccharification and fermentation of grass juice to bioethanol714493
food industryCryptococcus aureus-production of fuel ethanol and ultra-high fructose syrup690488
food industryKluyveromyces marxianus-a typical solution product consists of a mixture of fructose (155 g/l), glucose (155 g/l), sucrose (132 g/l) and fructooligosaccharides (50 g/l). The concentrations are suitable for applications in most food industries, in products such as sweets, candies, chocolates and yogurts. Besides, the prebiotic function of fructooligosaccharides as stimulants of the beneficial intestinal flora will give the product a functional and differentiated feature679967
nutritionPenicillium sp.Q8J0G1production of fructose syrup655019
diagnosticsTriticum aestivum-expression of 1-FEH w3 may be a good indicator to identify potential grain yield in wheat exposed to water deficits during grain filling, when linked to osmotic potential and green leaf retention705947
synthesisKluyveromyces marxianus-the enzyme preparation is used to hydrolyze pure inulin and raw inulin from Asparagus racemosus for the preparation of a high-fructose syrup681357
additional informationPenicillium sp.Q8J0G1fuel production655019

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISMLINK TO PUBMEDSOURCE
136519Norman, J.M.; Bunny, K.L.; Giffard, P.M.Characterization of levJ, a sucrase/fructanase-encoding gene from Actinomyces naeslundii T14V, and comparison of its product with other sucrose-cleaving enzymesGene15293-981995Actinomyces naeslundii PubMed
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208818Takahashi, N.; Mizuno, F.; Takamori, K.Purification and preliminary characterization of exo-beta-D-fructosidase in Streptococcus salivarius KTA-19Infect. Immun.47271-2761985Streptococcus salivarius PubMed
208819Frehner, M.; Keller, F.; Wiemken, A.Localisation of fructan metabolism in the vacuoles isolated from protoplasts of jerusalem artichoke tubers (Helianthus tuberosus L.)J. Plant Physiol.116197-2081984Helianthus tuberosus-
208820Igarashi, T.; Yakamoto, A.; Goto, N.Characterization of an exo-beta-D-fructosidase from Streptococcus mutants ingbrittMicrobiol. Immunol.36643-6471992Streptococcus mutans PubMed
208821Burne, R.A.; Penders, J.E.C.Characterization of the Streptococcus mutans GS-5 fruA gene encoding exo-beta-D-fructosidaseInfect. Immun.604621-46321992Streptococcus mutans PubMed
208823Henson, C.A.; Livingston III, D.P.Purification and characterization of an oat fructan exohydrolase that preferentially hydrolyzes beta-2,6-fructansPlant Physiol.110639-6441996Avena sativa PubMed
208824Henson, C.A.; Livingston III, D.P.Characterization of a fructan exohydrolase purified from barley stems that hydrolyzes multiple fructofuranosidic linkagesPlant Physiol. Biochem.36715-7201998Hordeum vulgare-
208825De Roover, J.; Van Laere, A.; De Winter, M.; Timmermanns, J.W.; Van den Ende, W.Purification and properties of a second fructan exohydrolase from the roots of Chicorium intybusPhysiol. Plant.10628-341999Cichorium intybus-
654116Verhaest, M.; Van den Ende, W.; Yoshida, M.; Le Roy, K.; Peeraer, Y.; Sansen, S.; De Ranter, C.J.; Van Laere, A.; Rabijns, A.Crystallization and preliminary X-ray diffraction study of fructan 1-exohydrolase IIa from Cichorium intybusActa Crystallogr. Sect. D60553-5542004Cichorium intybus PubMed
654218Gill, P.K.; Manhas, R.K.; Singh, J.; Singh, P.Purification and characterization of an exoinulinase from Aspergillus fumigatusAppl. Biochem. Biotechnol.11719-322004Aspergillus fumigatus PubMed
654875Kulminskaya, A.A.; Arand, M.; Eneyskaya, E.V.; Ivanen, D.R.; Shabalin, K.A.; Shishlyannikov, S.M.; Saveliev, A.N.; Korneeva, O.S.; Neustroev, K.N.Biochemical characterization of Aspergillus awamori exoinulinase: substrate binding characteristics and regioselectivity of hydrolysisBiochim. Biophys. Acta165022-292003Aspergillus awamori PubMed
655019Moriyama, S.; Akimoto, H.; Suetsugu, N.; Kawasaki, S.; Nakamura, T.; Ohta, K.Purification and properties of an extracellular exoinulinase from Penicillium sp. strain TN-88 and sequence analysis of the encoding geneBiosci. Biotechnol. Biochem.661887-18962002Penicillium sp. PubMed
655298Jing, W.; Zhengyu, J.; Bo, J.; Xueming, X.Separation and identification of exo- and endoinulinase from Aspergillus ficuumCurr. Microbiol.47109-1122003Aspergillus ficuum PubMed
655714Hamdy, H.S.Purification and some important characters of extracellular inulinase of Alternaria alternata (Fr.) KeisslerIndian J. Exp. Biol.401393-13982002Alternaria alternata PubMed
656406Michiels, A.; Van Laere, A.; Van den Ende, W.; Tucker, M.Expression analysis of a chicory fructan 1-exohydrolase gene reveals complex regulation by coldJ. Exp. Bot.551325-13332004Cichorium intybus PubMed
656993Van den Ende, W.; Michiels, A.; De Roover, J.; Verhaert, P.; Van Laere, A.Cloning and functional analysis of chicory root fructan1-exohydrolase I (1-FEH I): a vacuolar enzyme derived from a cell-wall invertase ancestor? Mass fingerprint of the 1-FEH I enzymePlant J.24447-4562000Cichorium intybus PubMed
657006Van den Ende, W.; De Coninck, B.; Clerens, S.; Vergauwen, R.; Van Laere, A.Unexpected presence of fructan 6-exohydrolases (6-FEHs) in non-fructan plants: characterization, cloning, mass mapping and functional analysis of a novel 'cell-wall invertase-like' specific 6-FEH from sugar beet (Beta vulgaris L.)Plant J.36697-7102003Beta vulgaris PubMed
657033Van den Ende, W.; Michiels, A.; Van Wonterghem, D.; Clerens, S.P.; De Roover, J.; Van Laere, A.J.Defoliation induces fructan 1-exohydrolase II in witloof chicory roots. Cloning and purification of two isoforms, fructan 1-exohydrolase IIa and fructan 1-exohydrolase IIb. Mass fingerprint of the fructan 1-exohydrolase II enzymesPlant Physiol.1261186-11952001Cichorium intybus PubMed
657048Van Den Ende, W.; Clerens, S.; Vergauwen, R.; Van Riet, L.; Van Laere, A.; Yoshida, M.; Kawakami, A.Fructan 1-exohydrolases. beta-(2,1)-trimmers during graminan biosynthesis in stems of wheat? Purification, characterization, mass mapping, and cloning of two fructan 1-exohydrolase isoformsPlant Physiol.131621-6312003Triticum aestivum PubMed
657274Zhang, L.; Zhao, C.; Zhu, D.; Ohta, Y.; Wang, Y.Purification and characterization of inulinase from Aspergillus niger AF10 expressed in Pichia pastorisProtein Expr. Purif.35272-2752004Aspergillus niger PubMed
657381Paludan-Muller, C.; Gram, L.; Rattray, F.P.Purification and characterisation of an extracellular fructan beta-fructosidase from a Lactobacillus pentosus strain isolated from fermented fishSyst. Appl. Microbiol.2513-202002Lactobacillus pentosus PubMed
657429Uzunova, K.; Vassileva, A.; Kambourova, M.; Ivanova, V.; Spasova, D.; Mandeva, R.; Derekova, A.; Tonkova, A.Production and properties of a bacterial thermostable exo-inulinaseZ. Naturforsch. C561022-10282001Bacillus sp. PubMed
660179Verhaest, M.; Ende, W.V.; Roy, K.L.; De Ranter, C.J.; Laere, A.V.; Rabijns, A.X-ray diffraction structure of a plant glycosyl hydrolase family 32 protein: fructan 1-exohydrolase IIa of Cichorium intybusPlant J.41400-4112005Cichorium intybus PubMed
660316Yang, J.; Zhang, J.; Wang, Z.; Zhu, Q.; Liu, L.Activities of fructan- and sucrose-metabolizing enzymes in wheat stems subjected to water stress during grain fillingPlanta220331-3432004Triticum aestivum PubMed
665885Benkeblia, N.; Ueno, K.; Onodera, S.; Shiomi, N.Variation of fructooligosaccharides and their metabolizing enzymes in onion bulb (Allium cepa L. cv. Tenshin) during long-term storageJ. Food Sci.70S208-S2142005Allium cepa-
668724Kawakami, A.; Yoshida, M.; Van den Ende, W.Molecular cloning and functional analysis of a novel 6&1-FEH from wheat (Triticum aestivum L.) preferentially degrading small graminans like bifurcoseGene35893-1012005Triticum aestivum PubMed
669668Van Riet, L.; Nagaraj, V.; Van den Ende, W.; Clerens, S.; Wiemken, A.; van Laere, A.Purification, cloning and functional characterization of a fructan 6-exohydrolase from wheat (Triticum aestivum L.)J. Exp. Bot.57213-2232006Triticum aestivum PubMed
669841Nagem, R.A.; Rojas, A.L.; Golubev, A.M.; Korneeva, O.S.; Eneyskaya, E.V.; Kulminskaya, A.A.; Neustroev, K.N.; Polikarpov, I.Crystal structure of exo-inulinase from Aspergillus awamori: the enzyme fold and structural determinants of substrate recognitionJ. Mol. Biol.344471-4802004Aspergillus awamori PubMed
670539De Coninck, B.; Le Roy, K.; Francis, I.; Clerens, S.; Vergauwen, R.; Halliday, A.M.; Smith, S.M.; Van Laere, A.; van den Ende, W.Arabidopsis AtcwINV3 and 6 are not invertases but are fructan exohydrolases (FEHs) with different substrate specificitiesPlant Cell Environ.28432-4432005Arabidopsis thaliana-
670618Portes, M.T.; Carvalho, M.A.Spatial distribution of fructans and fructan metabolizing enzymes in rhizophores of Vernonia herbacea (Vell.) Rusby (Asteraceae) in different developmental phasesPlant Sci.170624-6332006Vernonia herbacea-
670976Van den Ende, W.; De Coninck, B.; Van Laere, A.Plant fructan exohydrolases: a role in signaling and defense?Trends Plant Sci.9523-5282004Arabidopsis thaliana, Beta vulgaris, Cichorium intybus PubMed
678752Singh, R.S.; Sooch, B.S.; Puri, M.Optimization of medium and process parameters for the production of inulinase from a newly isolated Kluyveromyces marxianus YS-1Biores. Technol.982518-25252007Kluyveromyces marxianus PubMed
678762Singh, R.S.; Bhermi, H.K.Production of extracellular exoinulinase from Kluyveromyces marxianus YS-1 using root tubers of Asparagus officinalisBiores. Technol.997418-74232008Kluyveromyces marxianus PubMed
679966Zhang, W.; Ge, X.Improvement of fructanohydrolase production in Aspergillus niger SL-09 by sucrose esterFood Technol. Biotechnol.4459-642006Aspergillus niger-
679967Santos, A.M.; Maugeri, F.Synthesis of fructooligosaccharides from sucrose using inulinase from Kluyveromyces marxianusFood Technol. Biotechnol.45181-1862007Kluyveromyces marxianus-
680946Moriyama, S.; Muguruma, M.; Ohta, K.Quantitative expression analysis of inulinase gene cluster of Penicillium sp. strain TN-88J. Biosci. Bioeng.101277-2792006Penicillium sp. PubMed
680950Moriyama, S.; Ohta, K.Functional characterization and evolutionary implication of the internal 157-amino-acid sequence of an exoinulinase from Penicillium sp. strain TN-88J. Biosci. Bioeng.103293-2972007Penicillium sp. PubMed
681133Lothier, J.; Lasseur, B.; Le Roy, K.; Van Laere, A.; Prudhomme, M.P.; Barre, P.; Van den Ende, W.; Morvan-Bertrand, A.Cloning, gene mapping, and functional analysis of a fructan 1-exohydrolase (1-FEH) from Lolium perenne implicated in fructan synthesis rather than in fructan mobilizationJ. Exp. Bot.581969-19832007Lolium perenne PubMed
681161Narai-Kanayama, A.; Tokita, N.; Aso, K.Dependence of fructooligosaccharide content on activity of fructooligosaccharide-metabolizing enzymes in yacon (Smallanthus sonchifolius) tuberous roots during storageJ. Food Sci.72S381-S3872007Smallanthus sonchifolius PubMed
681217Gong, F.; Sheng, J.; Chi, Z.; Li, J.Inulinase production by a marine yeast Pichia guilliermondii and inulin hydrolysis by the crude inulinaseJ. Ind. Microbiol. Biotechnol.34179-1852007Meyerozyma guilliermondii PubMed
681357Singh, R.S.; Dhaliwal, R.; Puri, M.Partial purification and characterization of exoinulinase from Kluyveromyces marxianus YS-1 for preparation of high-fructose syrupJ. Microbiol. Biotechnol.17733-7382007Kluyveromyces marxianus PubMed
681774Gao, L.; Chi, Z.; Sheng, J.; Wang, L.; Li, J.; Gong, F.Inulinase-producing marine yeasts: evaluation of their diversity and inulin hydrolysis by their crude enzymesMicrob. Ecol.54722-7292007Cryptococcus aureus, Debaryomyces hansenii, Meyerozyma guilliermondii PubMed
682058Pessoni, R.A.; Braga, M.R.; Figueiredo-Ribeiro, R.d.e.C.Purification and properties of exo-inulinases from Penicillium janczewskii growing on distinct carbon sourcesMycologia99493-5032007Penicillium janczewskii PubMed
682143Verhaest, M.; Lammens, W.; Le Roy, K.; De Ranter, C.J.; Van Laere, A.; Rabijns, A.; Van den Ende, W.Insights into the fine architecture of the active site of chicory fructan 1-exohydrolase: 1-kestose as substrate vs sucrose as inhibitorNew Phytol.17490-1002007Cichorium intybus PubMed
682145Le Roy, K.; Verhaest, M.; Rabijns, A.; Clerens, S.; Van Laere, A.; Van den Ende, W.N-glycosylation affects substrate specificity of chicory fructan 1-exohydrolase: evidence for the presence of an inulin binding cleftNew Phytol.176317-3242007Cichorium intybus PubMed
682147Le Roy, K.; Lammens, W.; Van Laere, A.; Van den Ende, W.Influencing the binding configuration of sucrose in the active sites of chicory fructan 1-exohydrolase and sugar beet fructan 6-exohydrolaseNew Phytol.178572-5802008Cichorium intybus PubMed
682426Le Roy, K.; Lammens, W.; Verhaest, M.; De Coninck, B.; Rabijns, A.; Van Laere, A.; Van den Ende, W.Unraveling the difference between invertases and fructan exohydrolases: a single amino acid (Asp-239) substitution transforms Arabidopsis cell wall invertase1 into a fructan 1-exohydrolasePlant Physiol.145616-6252007Arabidopsis thaliana PubMed
690488Sheng, J.; Chi, Z.; Gong, F.; Li, J.Purification and Characterization of Extracellular Inulinase from a Marine Yeast Cryptococcus aureus G7a and Inulin Hydrolysis by the Purified InulinaseAppl. Biochem. Biotechnol.144111-1212008Cryptococcus aureus PubMed
690509Mutanda, T.; Wilhelmi, B.; Whiteley, C.G.Controlled production of fructose by an exoinulinase from Aspergillus ficuumAppl. Biochem. Biotechnol.15965-772009Aspergillus ficuum PubMed
705947Zhang, J.; Dell, B.; Conocono, E.; Waters, I.; Setter, T.; Appels, R.Water deficits in wheat: Fructan exohydrolase (1-FEH) mRNA expression and relationship to soluble carbohydrate concentrations in two varietiesNew Phytol.181843-8502009Triticum aestivum-
714493Martel, C.M.; Parker, J.E.; Jackson, C.J.; Warrilow, A.G.; Rolley, N.; Greig, C.; Morris, S.M.; Donnison, I.S.; Kelly, D.E.; Kelly, S.L.Expression of bacterial levanase in yeast enables simultaneous saccharification and fermentation of grass juice to bioethanolBiores. Technol.1021503-15082011Bacillus subtilis PubMed
714499Arrizon, J.; Morel, S.; Gschaedler, A.; Monsan, P.Purification and substrate specificities of a fructanase from Kluyveromyces marxianus isolated from the fermentation process of MezcalBiores. Technol.1023298-33032011Kluyveromyces marxianus PubMed
714715Ueno, K.; Ishiguro, Y.; Yoshida, M.; Onodera, S.; Shiomi, N.Cloning and functional characterization of a fructan 1-exohydrolase (1-FEH) in edible burdock (Arctium lappa L.)Chem. Cent. J.5162011Arctium lappa PubMed
715762Tamura, K.I.; Sanada, Y.; Tase, K.; Komatsu, T.; Yoshida, M.Pp6-FEH1 encodes an enzyme for degradation of highly polymerized levan and is transcriptionally induced by defoliation in timothy (Phleum pratense L.)J. Exp. Bot.623421-34312011Phleum pratense PubMed
716041Kawakami, A.; Yoshida, M.Graminan breakdown by fructan exohydrolase induced in winter wheat inoculated with snow moldJ. Plant Physiol.169294-3022012Triticum aestivum PubMed

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