Information on EC 3.2.1.64 - 2,6-beta-fructan 6-levanbiohydrolase:
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EC NUMBERCOMMENTARY
3.2.1.64-

RECOMMENDED NAMEGeneOntology No.
2,6-beta-fructan 6-levanbiohydrolaseGO:0033912

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
Hydrolysis of (2->6)-beta-D-fructofuranan, to remove successive disaccharide residues as levanbiose, i.e. 6-(beta-D-fructofuranosyl)-D-fructose, from the end of the chain
show the reaction diagram		----

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
hydrolysis of O-glycosyl bond----

PATHWAYKEGG LinkMetaCyc Link
No entries in this field

SYSTEMATIC NAMEIUBMB Comments
(2->6)-beta-D-fructofuranan 6-(beta-D-fructosyl)-D-fructose-hydrolase-

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
2,6-beta-D-fructan 6-levanbiohydrolase----
2,6-beta-fructan 6-levanbiohydrolase----
beta2,6-fructan-6-levanbiohydrolase----
LDE----
levanbiohydrolase, 2,6-beta-fructan 6-----

CAS REGISTRY NUMBERCOMMENTARY
37288-46-3-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Candida albicans 11strain 11136754--Manually annotated by BRENDA team
Microbacterium laevaniformansstrain ATCC 15953651408--Manually annotated by BRENDA team
Pseudomonas sp.strain 11136754--Manually annotated by BRENDA team
Streptomyces exfoliatusstrain F3-2136755--Manually annotated by BRENDA team
Streptomyces exfoliatusstrain F3-2651377Q8GI79SwissProtManually annotated by BRENDA team
Streptomyces exfoliatus F3-2strain F3-2136755--Manually annotated by BRENDA team
Streptomyces exfoliatus F3-2strain F3-2651377Q8GI79SwissProtManually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
1-fructosylnystose + H2Obeta-D-fructose + alpha-D-glucose + sucrose + 1-kestose + nystose
show the reaction diagram		Streptomyces exfoliatus-at 81.4% the rate of levan136755-136755?
1-kestose + H2O?
show the reaction diagram		Microbacterium laevaniformans-0.2% of the activity with levan from Serratia sp.651408-651408?
1-kestose + H2Osucrose + beta-D-fructose + alpha-D-glucose
show the reaction diagram		Streptomyces exfoliatus-at 33.3% the rate of levan136755-136755?
inulin + H2O?
show the reaction diagram		Microbacterium laevaniformans-5% of the activity with levan from Serratia sp.651408-651408?
levan + H2Olevanbiose
show the reaction diagram		Pseudomonas sp.-levan i.e. 2,6-beta-D-fructan1367546-beta-fructosylfructose136754?
levan + H2Olevanbiose
show the reaction diagram		Streptomyces exfoliatus-levan i.e. 2,6-beta-D-fructan, smallest substrate to be hydrolyzed is levantriose1367556-beta-fructosylfructose136755?
nystose + H2O?
show the reaction diagram		Microbacterium laevaniformans-0.5% of the activity with levan from Serratia sp.651408-651408?
nystose + H2Obeta-D-fructose + alpha-D-glucose + sucrose + 1-kestose
show the reaction diagram		Streptomyces exfoliatus-at 73.4% the rate of levan136755-136755?
sucrose + H2Obeta-D-fructose + alpha-D-glucose
show the reaction diagram		Streptomyces exfoliatus-at 6.5% the rate of levan136755-136755?
levan + H2Olevanbiose + ?
show the reaction diagram		Microbacterium laevaniformans-enzyme activity depends on source of levan, sources in decreasing order of activity: Serratia sp., Rhanella aquatilis, Zymomonas mobilis, M. laevaniformans651408-651408?
additional information?-Microbacterium laevaniformans-enzyme cleaves beta-2,6-linkage of levan and beta-2,1-linkage of fructooligosaccharides, no substrate are: soluble starch, cellobiose, sucrose, maltose, melibiose, raffinose, lactose651408-651408?

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
levan + H2Olevanbiose
show the reaction diagram		Pseudomonas sp.-levan i.e. 2,6-beta-D-fructan1367546-beta-fructosylfructose136754
levan + H2Olevanbiose
show the reaction diagram		Streptomyces exfoliatus-levan i.e. 2,6-beta-D-fructan1367556-beta-fructosylfructose136755

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
No entries in this field

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
No entries in this field

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
105-Streptomyces exfoliatus--136755
997.3-Microbacterium laevaniformans-pH 6.0, 30°C651408

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
5.5-Streptomyces exfoliatus--136755
6-Microbacterium laevaniformans--651408

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
5.57Microbacterium laevaniformans-more than 80% of maximum activity in this range651408

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
30-Microbacterium laevaniformans--651408

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
culture supernatantPseudomonas sp.--136754Manually annotated by BRENDA team
culture supernatantStreptomyces exfoliatus--136755Manually annotated by BRENDA team

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
extracellularPseudomonas sp.---136754Manually annotated by BRENDA team
extracellularStreptomyces exfoliatus---136755Manually annotated by BRENDA team
extracellularMicrobacterium laevaniformans---651408Manually annotated by BRENDA team

PDBSCOPCATHORGANISM
No entries in this field

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
60000-Streptomyces exfoliatus-gel filtration136755

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
?Microbacterium laevaniformans-x * 68800, SDS-PAGE651408
monomerStreptomyces exfoliatus-1 * 54000, SDS-PAGE136755
additional informationStreptomyces exfoliatusQ8GI79N-terminal amino acid sequence651377

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
proteolytic modificationStreptomyces exfoliatusQ8GI79enzyme contains a signal peptide of 37 amino acids and a mature protein of 482 amino acids651377

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
3.58Streptomyces exfoliatus--136755

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
40-Microbacterium laevaniformans-30 min, 10% loss of activity651408
50-Streptomyces exfoliatus-stable up to136755
50-Microbacterium laevaniformans-30 min, 65% loss of activiy651408

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
recombinant protein, expression n Escherichia coliMicrobacterium laevaniformans-651408

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-Microbacterium laevaniformans-651408
-Streptomyces exfoliatusQ8GI79651377

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISMLINK TO PUBMEDSOURCE
136754Avigad, G.; Zelikson, R.Cleavage of fructans to levanbiose by a specific hydrolaseBull. Res. Counc. Isr.11A4253-2571963Pseudomonas sp.-
136755Saito, K.; Kondo, K.; Kojima, I.; Yokota, A.; Tomita, F.Purification and characterization of 2,6-beta-D-fructan 6-levanbiohydrolase from Streptomyces exfoliatus F3-2Appl. Environ. Microbiol.66252-2562000Streptomyces exfoliatus PubMed
651377Saito, K.; Oda, Y.; Tomita, F.; Yokota, A.Molecular cloning of the gene for 2,6-beta-D-fructan 6-levanbiohydrolase from Streptomyces exfoliatus F3-2FEMS Microbiol. Lett.218265-2702003Streptomyces exfoliatus PubMed
651408Song, E.K.; Kim, H.; Sung, H.K.; Cha, J.Cloning and characterization of a levanbiohydrolase from Microbacterium laevaniformans ATCC 15953Gene29145-552002Microbacterium laevaniformans PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 3.2.1.64)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)