Information on EC 3.2.1.134 - difructose-anhydride synthase:
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The lowest common taxonomy group for this enzyme is: Aspergillus fumigatus

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EC NUMBERCOMMENTARY
3.2.1.134-

RECOMMENDED NAMEGeneOntology No.
difructose-anhydride synthaseGO:0047853

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
bis-D-fructose 2',1:2,1'-dianhydride + H2O = inulobiose
show the reaction diagram		----

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
hydrolysis of O-glycosyl bond----

PATHWAYKEGG LinkMetaCyc Link
No entries in this field

SYSTEMATIC NAMEIUBMB Comments
bis-D-fructose 2',1:2,1'-dianhydride fructohydrolaseProduces difructose anhydride by the reverse reaction of partial hydrolysis, forming an alpha-fructosidic linkage.

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
hydrolase, inulobiose----

CAS REGISTRY NUMBERCOMMENTARY
121479-55-8-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Aspergillus fumigatus-646807, 646808--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
inulobiosebis-D-fructose 2',1:2,1' dianhydride + H2O
show the reaction diagram		Aspergillus fumigatus--646808-646808-
inulobiosebis-D-fructose 2',1:2,1' dianhydride + H2O
show the reaction diagram		Aspergillus fumigatus--646807-646807r
inulobiosebis-D-fructose 2',1:2,1' dianhydride + H2O
show the reaction diagram		Aspergillus fumigatus-might act as a regulator for a piecemeal use of inulin as a carbon source646807--r

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
inulobiosebis-D-fructose 2',1:2,1' dianhydride + H2O
show the reaction diagram		Aspergillus fumigatus-might act as a regulator for a piecemeal use of inulin as a carbon source646807--

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
No entries in this field

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
No entries in this field

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
Co2+Aspergillus fumigatus--646807 2D-image
Cu2+Aspergillus fumigatus--646807 2D-image
DTTAspergillus fumigatus-slight646807 2D-image
L-cysteineAspergillus fumigatus--646807 2D-image
Zn2+Aspergillus fumigatus--646807 2D-image

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
241-bis-D-fructose 2',1:2,1' dianhydrideAspergillus fumigatus-pH 6, 37°C646807 2D-image
377-InulobioseAspergillus fumigatus-pH 6, 37°C646807 2D-image

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
6-Aspergillus fumigatus--646807

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
48-Aspergillus fumigatus--646807

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
4.5-Aspergillus fumigatus-pH gradient 3.5-10646808

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
myceliumAspergillus fumigatus--646807, 646808Manually annotated by BRENDA team

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
No entries in this field

PDBSCOPCATHORGANISM
No entries in this field

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
68.6Aspergillus fumigatus-stable at 37°C646807

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
50-Aspergillus fumigatus-stable up to 50°C at pH 6646807

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-24°C, stable for several monthsAspergillus fumigatus-646808
under toluene in the refrigerator at pH 6.0, stable for several monthsAspergillus fumigatus-646807

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-Aspergillus fumigatus-646808

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISMLINK TO PUBMEDSOURCE
646807Matsuyama, T.; Tanaka, K.On the enzyme of Aspergillus fumigatus producing difructose anhydride I from inulobioseAgric. Biol. Chem.53831-8321989Aspergillus fumigatus-
646808Matsuyama, T.; Tanaka, K.; Mashiko, M.; Kanamoto, M.Enzymic formation of di-D-fructose 1,2; 2,1 dianhydride from inulobiose by Aspergillus fumigatusJ. Biochem.921325-13281982Aspergillus fumigatus PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 3.2.1.134)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)