Information on EC 3.1.7.6 - farnesyl diphosphatase:
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EC NUMBERCOMMENTARY
3.1.7.6-

RECOMMENDED NAMEGeneOntology No.
farnesyl diphosphatase-

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
(2E,6E)-farnesyl diphosphate + H2O = (2E,6E)-farnesol + diphosphate
show the reaction diagram		----
(2E,6E)-farnesyl diphosphate + H2O = (2E,6E)-farnesol + diphosphate
show the reaction diagram		-Saccharomyces cerevisiae-701757

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

PATHWAYKEGG LinkMetaCyc Link
all-trans-farnesol biosynthesis-PWY-6859
juvenile hormone III biosynthesis II-PWY-6650

SYSTEMATIC NAMEIUBMB Comments
(2E,6E)-farnesyl-diphosphate diphosphohydrolaseThe enzyme is involved in the biosynthesis of acyclic sesquiterpenoids [1].

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
farnesyl diphosphataseOryza sativa--656974
farnesyl pyrophosphataseRattus norvegicus--701894
farnesyl pyrophosphataseCricetulus griseus--701896
FPPaseRattus norvegicus--701894
FPPaseCricetulus griseus--701896
Pho8DELTA62Saccharomyces cerevisiae-the purified phosphatase is a truncated Pho8 lacking 62 amino acids at the N-terminus and is designated Pho8DELTA62N701757
pNPP/FPP phosphataseSaccharomyces cerevisiae--701757

CAS REGISTRY NUMBERCOMMENTARY
No entries in this field

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Candida albicansstrain A72701723--Manually annotated by BRENDA team
Candida albicans A72strain A72701723--Manually annotated by BRENDA team
Cricetulus griseus-701896--Manually annotated by BRENDA team
Datura stramonium-654930--Manually annotated by BRENDA team
Oryza sativacv. Nakdong656974--Manually annotated by BRENDA team
Rattus norvegicus-701894, 95169--Manually annotated by BRENDA team
Saccharomyces cerevisiae-701757--Manually annotated by BRENDA team
Uncaria tomentosa-706169--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate + H2O(2E,6E)-farnesol + diphosphate
show the reaction diagram		Cricetulus griseus--701896--?
(2E,6E)-farnesyl diphosphate + H2O(2E,6E)-farnesol + diphosphate
show the reaction diagram		Rattus norvegicus--701894--?
(2E,6E)-farnesyl diphosphate + H2O(2E,6E)-farnesol + diphosphate
show the reaction diagram		Oryza sativa--656974diphosphate is not determined-?
(2E,6E)-farnesyl diphosphate + H2O(2E,6E)-farnesol + diphosphate
show the reaction diagram		Rattus norvegicus--701894diphosphate not determined-?
(2E,6E)-farnesyl diphosphate + H2O(2E,6E)-farnesol + diphosphate
show the reaction diagram		Candida albicans--701723diphosphate not determined-?
(2E,6E)-farnesyl diphosphate + H2O(2E,6E)-farnesol + diphosphate
show the reaction diagram		Datura stramonium--654930diphosphate not determined-?
(2E,6E)-farnesyl diphosphate + H2O(2E,6E)-farnesol + diphosphate
show the reaction diagram		Rattus norvegicus-biosynthesis of acyclic sesquiterpenoids95169--?
(2E,6E)-farnesyl diphosphate + H2O(2E,6E)-farnesol + diphosphate
show the reaction diagram		Saccharomyces cerevisiae-biosynthesis of acyclic sesquiterpenoids701757--?
(2E,6E)-farnesyl diphosphate + H2O(2E,6E)-farnesol + diphosphate
show the reaction diagram		Rattus norvegicus-diphosphate is the major product, traces of phosphate. The amount of release of diphosphate is approximately equal to the amount of release of farnesol95169--?
(2E,6E)-farnesyl diphosphate + H2O(2E,6E)-farnesol + diphosphate
show the reaction diagram		Saccharomyces cerevisiae-the enzyme also hydrolyzes geranyl diphosphate and geranylgeranyl diphosphate to the corresponding alcohols701757--?
p-nitrophenyl phosphate + H2Op-nitrophenol + phosphate
show the reaction diagram		Saccharomyces cerevisiae--701757--?

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
(2E,6E)-farnesyl diphosphate + H2O(2E,6E)-farnesol + diphosphate
show the reaction diagram		Rattus norvegicus--701894--
(2E,6E)-farnesyl diphosphate + H2O(2E,6E)-farnesol + diphosphate
show the reaction diagram		Rattus norvegicus-biosynthesis of acyclic sesquiterpenoids95169--
(2E,6E)-farnesyl diphosphate + H2O(2E,6E)-farnesol + diphosphate
show the reaction diagram		Saccharomyces cerevisiae-biosynthesis of acyclic sesquiterpenoids701757--

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
No entries in this field

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
Ca2+Saccharomyces cerevisiae-2 mM, 2.2fold activation701757
Mg2+Uncaria tomentosa-activity is dependent on706169
Zn2+Saccharomyces cerevisiae-2 mM, 1.4fold activation701757
Mg2+Rattus norvegicus-slight stimulation95169
additional informationDatura stramonium-Mg2+-independent enzyme654930
additional informationOryza sativa-Mg2+-independent enzyme656974
additional informationSaccharomyces cerevisiae-2 mM CuSO4 shows no effect701757
additional informationRattus norvegicus-the activity is metal ion independent701894

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
(2E,6E)-farnesyl diphosphateCricetulus griseus-competitive701896 2D-image
3',5'-cAMPRattus norvegicus--95169 2D-image
arseniteRattus norvegicus-1 mM95169 2D-image
farnesonic acidRattus norvegicus--95169-
farnesyl diphosphateCricetulus griseus-0.25 mM, 80% inhibition701896 2D-image
Farnesyl monophosphateRattus norvegicus--701894 2D-image
Farnesyl monophosphateCricetulus griseus-0.07 mM, 35% inhibition701896 2D-image
geranyl diphosphateDatura stramonium-16% inhibition of soluble enzyme at 0.1 mM. 26% inhibition of microsomal enzyme at 0.05 mM654930 2D-image
geranyl diphosphateOryza sativa--656974 2D-image
geranylgeranyl diphosphateDatura stramonium-0.05 mM, 16% inhibition of soluble enzyme, 38% inhibition of microsomal enzyme654930 2D-image
geranylgeranyl diphosphateOryza sativa--656974 2D-image
geranylgeranyl diphosphateRattus norvegicus-potent noncompetitive inhibitor701894 2D-image
geranylgeranyl diphosphateCricetulus griseus-0.05 mM, 60% inhibition701896 2D-image
isopentenyl diphosphateRattus norvegicus-competitive95169 2D-image
Mg2+Saccharomyces cerevisiae-2 mM, 92% inhibition. 0.06 mM, no inhibition701757 2D-image
NaFCricetulus griseus-100 mM, about 70% inhibition701896 2D-image
phosphateSaccharomyces cerevisiae-2 mM, 85% inhibition701757 2D-image
Plasma albuminRattus norvegicus--95169-
zaragozic acid BRattus norvegicus-i.e. L694,581, mixed type inhibition at 0.04 mM. No inhibition by zaragozic acid A up to 0.1 mM701894-
Mn2+Saccharomyces cerevisiae-2 mM, 93% inhibition701757 2D-image
additional informationSaccharomyces cerevisiae-2 mM CuSO4 shows no effect701757-
additional informationRattus norvegicus-no inhibition by 1 mM p-mercuribenzoate95169-

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
ATPDatura stramonium-0.05, 8% stimulation654930 2D-image
ATPRattus norvegicus-0.93 mM, stimulates95169 2D-image
isopentenyl diphosphateDatura stramonium-20% stimulation of soluble enzyme at 0.1 mM, 20% stimulation of microsomal enzyme at 0.02 mM654930 2D-image
p-nitrophenyl phosphateDatura stramonium-0.05, 10% stimulation654930 2D-image
Triton X-100Saccharomyces cerevisiae-0.05 g/l, stimulates701757 2D-image

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.007-(2E,6E)-farnesyl diphosphateRattus norvegicus-pH 5.5, 37°C701894 2D-image
0.015-(2E,6E)-farnesyl diphosphateCricetulus griseus-pH 5.5, 37°C701896 2D-image
0.71.2(2E,6E)-farnesyl diphosphateRattus norvegicus-pH 8.8, 37°C95169 2D-image
additional information-additional informationRattus norvegicus-the KM-value for (2E,6E)-farnesyl diphosphate increases with an increase in pH701894-

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.37-(2E,6E)-farnesyl diphosphateCricetulus griseus-pH 5.5, 37°C701896 2D-image
0.1-geranyl diphosphateOryza sativa--656974 2D-image
0.005-geranylgeranyl diphosphateOryza sativa--656974 2D-image
5.2-isopentenyl diphosphateRattus norvegicus-pH 8.8, 37°C95169 2D-image

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.07-Farnesyl monophosphateRattus norvegicus-pH 5.5, 37°C701894 2D-image
0.005-geranylgeranyl diphosphateRattus norvegicus-pH 5.5, 37°C701894 2D-image
0.02-zaragozic acid BRattus norvegicus--701894-

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
0.0068-Rattus norvegicus--701894

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
5.2-Datura stramonium-microsomal enzyme654930
5.5-Rattus norvegicus--701894
5.5-Cricetulus griseus-assay at701896
6-Datura stramonium-soluble enzyme654930
6.8-Oryza sativa--656974
7-Saccharomyces cerevisiae--701757
8.8-Rattus norvegicus--95169

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
4.56.8Datura stramonium-pH 4.5: about 55% of maximal activity, pH 6.8: about 60% of maximal activit, soluble enzyme654930
56.2Rattus norvegicus-pH 5.0: about 45% of maximal activity, pH 6.2: about 75% of maximal activity701894
56.8Datura stramonium-pH 5.0: about 60% of maximal activity, pH 6.8: about 65% of maximal activit, microsomal enzyme654930
6.37Oryza sativa-pH 6.3: about 80% of maximal activity, pH 7.0: about about 70% of maximal activity656974
7-Saccharomyces cerevisiae-maximal activity at pH 7.0, less than 1% of maximal activity at pH 6.0, less than 10% of maximal activity at pH 8.5701757
8.59.1Rattus norvegicus-pH 8.5: about 40% of maximal activity, pH 9.1: about 65% of maximal activity95169

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
30-Saccharomyces cerevisiae-assay at701757
37-Cricetulus griseus-assay at701896

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
cell suspension cultureUncaria tomentosa--706169Manually annotated by BRENDA team
flowerDatura stramonium-soluble farnesyl diphosphatase is highest in fruit and flower followed by root and leaf654930Manually annotated by BRENDA team
fruitDatura stramonium-soluble farnesyl diphosphatase is highest in fruit and flower followed by root and leaf654930Manually annotated by BRENDA team
leafDatura stramonium-soluble farnesyl diphosphatase is highest in fruit and flower followed by root and leaf654930Manually annotated by BRENDA team
liverRattus norvegicus--701894Manually annotated by BRENDA team
rootDatura stramonium-soluble farnesyl diphosphatase is highest in fruit and flower followed by root and leaf. Root has the highest level of microsomal enzyme654930Manually annotated by BRENDA team
seedlingOryza sativa--656974Manually annotated by BRENDA team
testisRattus norvegicus--95169Manually annotated by BRENDA team

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
microsomeDatura stramonium-constitutively expressed at a high level in the soluble and the microsomal fraction-654930Manually annotated by BRENDA team
microsomeOryza sativa---656974Manually annotated by BRENDA team
microsomeRattus norvegicus---701894, 95169Manually annotated by BRENDA team
solubleDatura stramonium-constitutively expressed at a high level in the soluble and the microsomal fraction-654930Manually annotated by BRENDA team

PDBSCOPCATHORGANISM
No entries in this field

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
?Saccharomyces cerevisiae-x * 60000, SDS-PAGE701757

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
No entries in this field

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
partialRattus norvegicus-95169
-Saccharomyces cerevisiae-701757

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
constitutively expressed at a high level in the soluble and the microsomal fractionDatura stramonium-654930
maximally induced 36 h after UV-C irradiation and decreased until 60 hOryza sativa-656974
the flux of farnesyl diphosphate to farnesol is two times lower in cells elicited with pectin treatment than in control cellsUncaria tomentosa-706169

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
upCricetulus griseus-enzyme activity increases under conditions of increased metabolic flow through the isoprenoid pathway701896

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISMLINK TO PUBMEDSOURCE
95169Tsai, S.C.; Gaylor, J.L.Testicular sterols. V. Preparation and partial purification of a microsomal prenol pyrophosphate pyrophophohydrolaseJ. Biol. Chem.2414043-40501966Rattus norvegicus PubMed
654930Ha, S.B.; Lee, D.E.; Lee, H.J.; Song, S.J.; Back, K.Activities of soluble and microsomal farnesyl diphosphatases in Datura stramoniumBiol. Plant.47477-4792003Datura stramonium-
656974Nah, J.; Song, S.J.; Back, K.Partial characterization of farnesyl and geranylgeranyl diphosphatases induced in rice seedlings by UV-C irradiationPlant Cell Physiol.42864-8672001Oryza sativa PubMed
701723Hornby, J.M.; Kebaara, B.W.; Nickerson, K.W.Farnesol biosynthesis in Candida albicans: cellular response to sterol inhibition by zaragozic acid BAntimicrob. Agents Chemother.472366-23692003Candida albicans PubMed
701757Song, L.A soluble form of phosphatase in Saccharomyces cerevisiae capable of converting farnesyl diphosphate into E,E-farnesolAppl. Biochem. Biotechnol.128149-1582006Saccharomyces cerevisiae PubMed
701894Bansal, V.S.; Vaidya, S.Characterization of two distinct allyl pyrophosphatase activities from rat liver microsomesArch. Biochem. Biophys.315393-3991994Rattus norvegicus PubMed
701896Meigs, T.E.; Simoni, R.D.Farnesol as a regulator of HMG-CoA reductase degradation: characterization and role of farnesyl pyrophosphataseArch. Biochem. Biophys.3451-91997Cricetulus griseus PubMed
706169Flores-Sanchez, I.J.; Ortega-Lopez, J.M.; del Carmen Montes-Horcasitas, M.; Ramos-Valdivia, A.C.Biosynthesis of sterols and triterpenes in cell suspension cultures of Uncaria tomentosaPlant Cell Physiol.431502-15092002Uncaria tomentosa PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 3.1.7.6)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)