Information on EC 3.1.2.15 - ubiquitin thiolesterase:
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The expected taxonomic range for this enzyme is: Oryctolagus cuniculus

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EC NUMBERCOMMENTARY
3.1.2.15-

RECOMMENDED NAMEGeneOntology No.
ubiquitin thiolesteraseGO:0004221

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol
show the reaction diagram		----

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
hydrolysis of thioester----

PATHWAYKEGG LinkMetaCyc Link
No entries in this field

SYSTEMATIC NAMEIUBMB Comments
ubiquitin-C-terminal-thioester hydrolaseActs on esters formed between thiols such as dithiothreitol or glutathione and the C-terminal glycine residue of the polypeptide ubiquitin. Also acts on AMP-ubiquitin. May be the same as EC 3.4.19.12, ubiquitinyl hydrolase 1.

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

CAS REGISTRY NUMBERCOMMENTARY
86480-67-3-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Oryctolagus cuniculus-646088--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
ubiquitin-AMP + H2Oubiquitin + AMP
show the reaction diagram		Oryctolagus cuniculus--646088--?
ubiquitin-dithiothreitol thiolester + H2Oubiquitin + dithiothreitol
show the reaction diagram		Oryctolagus cuniculus-thiolester between ubiquitin carboxyl terminus and dithiothreitol646088-646088?
ubiquitin-glutathione thiolester + H2Oubiquitin + glutathione
show the reaction diagram		Oryctolagus cuniculus-thiolester between ubiquitin carboxyl terminus and glutathione646088--?

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
No entries in this field

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
No entries in this field

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
No entries in this field

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
iodoacetamideOryctolagus cuniculus-ubiquitin protects646088 2D-image
UbiquitinOryctolagus cuniculus-product inhibition646088 2D-image

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
additional information-Oryctolagus cuniculus--646088

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
7.6-Oryctolagus cuniculus-assay at646088

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
37-Oryctolagus cuniculus-assay at646088

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
reticulocyteOryctolagus cuniculus--646088Manually annotated by BRENDA team

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
No entries in this field

PDBSCOPCATHORGANISM
1cmx, downloadSCOP (1cmx)CATH (1cmx)Bos taurus, Cavia porcellus, Cricetulus griseus, Gallus gallus, Homo sapiens
1ixd, downloadSCOP (1ixd)CATH (1ixd)Homo sapiens
1nb8, downloadSCOP (1nb8)CATH (1nb8)Homo sapiens
1nbf, downloadSCOP (1nbf)CATH (1nbf)Homo sapiens
1uch, downloadSCOP (1uch)CATH (1uch)Homo sapiens
1w6v, downloadSCOP (1w6v)CATH (1w6v)Homo sapiens
1wh0, downloadSCOP (1wh0)CATH (1wh0)Homo sapiens
1whb, downloadSCOP (1whb)CATH (1whb)Homo sapiens
1yy6, downloadSCOP (1yy6)CATH (1yy6)Homo sapiens
1yze, downloadSCOP (1yze)CATH (1yze)Homo sapiens
2a9u, downloadSCOP (2a9u)CATH (2a9u)Homo sapiens
2ayn, downloadSCOP (2ayn)CATH (2ayn)Homo sapiens
2ayo, downloadSCOP (2ayo)CATH (2ayo)Homo sapiens
2dag, downloadSCOP (2dag)CATH (2dag)Homo sapiens
2dak, downloadSCOP (2dak)CATH (2dak)Homo sapiens
2f1w, downloadSCOP (2f1w)CATH (2f1w)Homo sapiens
2f1z, downloadSCOP (2f1z)CATH (2f1z)Homo sapiens
2foj, downloadSCOP (2foj)CATH (2foj)Homo sapiens
2foo, downloadSCOP (2foo)CATH (2foo)Homo sapiens
2fop, downloadSCOP (2fop)CATH (2fop)Homo sapiens
2g43, downloadSCOP (2g43)CATH (2g43)Homo sapiens
2g45, downloadSCOP (2g45)CATH (2g45)Homo sapiens
2gfo, downloadSCOP (2gfo)CATH (2gfo)Homo sapiens
2gwf, downloadSCOP (2gwf)CATH (2gwf)Homo sapiens
2hd5, downloadSCOP (2hd5)CATH (2hd5)Homo sapiens
2i50, downloadSCOP (2i50)CATH (2i50)Homo sapiens
2ibi, downloadSCOP (2ibi)CATH (2ibi)Homo sapiens
2kvr, downloadSCOP (2kvr)CATH (2kvr)Homo sapiens
2vhf, downloadSCOP (2vhf)CATH (2vhf)Homo sapiens
2y5b, downloadSCOP (2y5b)CATH (2y5b)Homo sapiens
2y6e, downloadSCOP (2y6e)CATH (2y6e)Homo sapiens
2znr, downloadSCOP (2znr)CATH (2znr)Homo sapiens
2znv, downloadSCOP (2znv)CATH (2znv)Homo sapiens
3i3t, downloadSCOP (3i3t)CATH (3i3t)Homo sapiens
3lmn, downloadSCOP (3lmn)CATH (3lmn)Homo sapiens
3mqr, downloadSCOP (3mqr)CATH (3mqr)Homo sapiens
3mqs, downloadSCOP (3mqs)CATH (3mqs)Homo sapiens
3mtn, downloadSCOP (3mtn)CATH (3mtn)Homo sapiens
3n3k, downloadSCOP (3n3k)CATH (3n3k)Homo sapiens
3nhe, downloadSCOP (3nhe)CATH (3nhe)Homo sapiens
3o65, downloadSCOP (3o65)CATH (3o65)Homo sapiens
1cmx, downloadSCOP (1cmx)CATH (1cmx)Mus musculus
1vdl, downloadSCOP (1vdl)CATH (1vdl)Mus musculus
1wgg, downloadSCOP (1wgg)CATH (1wgg)Mus musculus
3jyu, downloadSCOP (3jyu)CATH (3jyu)Mus musculus
1cmx, downloadSCOP (1cmx)CATH (1cmx)Oryctolagus cuniculus, Rattus norvegicus, Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
1vjv, downloadSCOP (1vjv)CATH (1vjv)Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
2qiy, downloadSCOP (2qiy)CATH (2qiy)Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
3m99, downloadSCOP (3m99)CATH (3m99)Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
3mhh, downloadSCOP (3mhh)CATH (3mhh)Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
3mhs, downloadSCOP (3mhs)CATH (3mhs)Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
1cmx, downloadSCOP (1cmx)CATH (1cmx)Sus scrofa, Xenopus laevis

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
30000-Oryctolagus cuniculus-gel filtration646088

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
No entries in this field

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-Oryctolagus cuniculus-646088

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISM (UNIPROT ACCESSION NO.)LINK TO PUBMEDSOURCE
646088Rose, I.A.; Warms, J.V.B.An enzyme with ubiquitin carboxy-terminal esterase activity from reticulocytesBiochemistry224234-42371983Oryctolagus cuniculus PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 3.1.2.15)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)