Information on EC 3.1.1.6 - acetylesterase:
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The expected taxonomic range for this enzyme is: Acinetobacter sp.

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EC NUMBERCOMMENTARY
3.1.1.6-

RECOMMENDED NAMEGeneOntology No.
acetylesteraseGO:0008126

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
an acetic ester + H2O = an alcohol + acetate
show the reaction diagram		----
an acetic ester + H2O = an alcohol + acetate
show the reaction diagram		-Aspergillus niger 113, Aspergillus niger 12, Aspergillus niger 14, Aspergillus niger 148, Aspergillus niger 20 OSM, Aspergillus niger 26, Aspergillus niger 307, Aspergillus niger 311, Aspergillus niger 3M43, Aspergillus niger 4M-147, Aspergillus niger 5-16, Aspergillus niger 5195, Aspergillus niger, Aspergillus niger 71, Aspergillus niger 92, Aspergillus niger A158, Aspergillus niger A-25, Aspergillus niger A-7420, Aspergillus niger Aa-20, Aspergillus niger AKU 3302, Aspergillus niger ATCC 9029, Aspergillus niger B03, Aspergillus niger B60, Aspergillus niger B, Aspergillus niger BCC14405, Aspergillus niger BRFM 131, Aspergillus niger CCIM, Aspergillus niger CDP-AI, Aspergillus niger CFR 1105, Aspergillus niger CS 180, Aspergillus niger CuZn-SOD, Aspergillus niger FS3, Aspergillus niger FTCC 5003, Aspergillus niger FTCC, Aspergillus niger GH1, Aspergillus niger GRM 3, Aspergillus niger HA37, Aspergillus niger I-1472, Aspergillus niger IFO31125, Aspergillus niger IFO 31628, Aspergillus niger IIIAn/8, Aspergillus niger IMI 303386, Aspergillus niger K10, Aspergillus niger KF-267, Aspergillus niger KI, Aspergillus niger LCF 8, Aspergillus niger M47, Aspergillus niger MIUG 16, Aspergillus niger MTCC 2425, Aspergillus niger MTCC F0101, Aspergillus niger N204, Aspergillus niger N400, Aspergillus niger N400(CBS 120-49), Aspergillus niger N402., Aspergillus niger N402, Aspergillus niger NCIM 1207, Aspergillus niger NCIM 548, Aspergillus niger NCIM 565, Aspergillus niger NFCCP, Aspergillus niger NIAB 280, Aspergillus niger NRRL 2270, Aspergillus niger NRRL 3135, Aspergillus niger NRRL3135., Aspergillus niger NRRL 326, Aspergillus niger NRRL 330, Aspergillus niger NRRL337, Aspergillus niger NRRL-3, Aspergillus niger NRRL3, Aspergillus niger NRRL 372, Aspergillus niger NRRL372, Aspergillus niger NRRL 4361, Aspergillus niger NRRL 65, Aspergillus niger PKL 104, Aspergillus niger R-27, Aspergillus niger RD6.13, Aspergillus niger RH5344, Aspergillus niger SA6, Aspergillus niger SL-09, Aspergillus niger T0005/007-2, Aspergillus niger T213., Aspergillus niger T213, Aspergillus niger Tiegh, Aspergillus niger UBC 814, Aspergillus niger UBC814, Aspergillus niger UV-11, Aspergillus niger ZD11-650815
an acetic ester + H2O = an alcohol + acetate
show the reaction diagram		active site serine, active site structure, reaction and ligand binding mechanismRhodococcus sp. 19070, Rhodococcus sp. 400, Rhodococcus sp., Rhodococcus sp. 7, Rhodococcus sp. A81, Rhodococcus sp. AA21, Rhodococcus sp. AIU Z-35-1, Rhodococcus sp. AJ270, Rhodococcus sp. AK32, Rhodococcus sp. AN-22, Rhodococcus sp. ATCC39484, Rhodococcus sp. B1, Rhodococcus sp. C1, Rhodococcus sp. C9, Rhodococcus sp. C-x, Rhodococcus sp. DK17, Rhodococcus sp. DX-35, Rhodococcus sp. ECU0066, Rhodococcus sp. F2-7-3, Rhodococcus sp. Fp2, Rhodococcus sp. G-74-2, Rhodococcus sp. GK1, Rhodococcus sp. H1, Rhodococcus sp. HR199, Rhodococcus sp. IGT8, Rhodococcus sp. IGTS8, Rhodococcus sp. IOC-77, Rhodococcus sp. J1, Rhodococcus sp. M4, Rhodococcus sp. M-750, Rhodococcus sp. M-777, Rhodococcus sp. N186/21, Rhodococcus sp. N595, Rhodococcus sp. N771, Rhodococcus sp. N-774, Rhodococcus sp. NCIMB 11215, Rhodococcus sp. NCIMB 11216, Rhodococcus sp. NCIMB 12038, Rhodococcus sp. NCIMB12038, Rhodococcus sp. NCIMB 9784, Rhodococcus sp. NDB 1165, Rhodococcus sp. Novo SP361, Rhodococcus sp. P1, Rhodococcus sp. R04, Rhodococcus sp. R312, Rhodococcus sp. RHA1, Rhodococcus sp. S13-4, Rhodococcus sp. SHZ-1, Rhodococcus sp. TK6-652394
an acetic ester + H2O = an alcohol + acetate
show the reaction diagram		-Alphacoronavirus, Alphacoronavirus SARS-CoV-652759
an acetic ester + H2O = an alcohol + acetate
show the reaction diagram		-Meyerozyma guilliermondii, Meyerozyma guilliermondii B10-05, Meyerozyma guilliermondii FTI 20037, Meyerozyma guilliermondii FTI, Meyerozyma guilliermondii H17, Meyerozyma guilliermondii NRRL Y-17257, Meyerozyma guilliermondii OUC1, Meyerozyma guilliermondii PYCC 3012, Meyerozyma guilliermondii UFMG-Y65, Meyerozyma guilliermondii Y-1017-653087
an acetic ester + H2O = an alcohol + acetate
show the reaction diagram		-Cymbopogon martinii-653450

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
acetylationCellvibrio japonicus, Cellvibrio japonicus NCIMB 10462, Clostridium thermocellum, Clostridium thermocellum 27405., Clostridium thermocellum 651, Clostridium thermocellum ATCC27405, Clostridium thermocellum CBH III, Clostridium thermocellum CelJ, Clostridium thermocellum F7, Clostridium thermocellum YM4, Clostridium thermocellum ZJL4--715029
hydrolysis of carboxylic ester----
hydrolysis of carboxylic esterAspergillus niger, Aspergillus niger 113, Aspergillus niger 12, Aspergillus niger 14, Aspergillus niger 148, Aspergillus niger 20 OSM, Aspergillus niger 26, Aspergillus niger 307, Aspergillus niger 311, Aspergillus niger 3M43, Aspergillus niger 4M-147, Aspergillus niger 5-16, Aspergillus niger 5195, Aspergillus niger 71, Aspergillus niger 92, Aspergillus niger A158, Aspergillus niger A-25, Aspergillus niger A-7420, Aspergillus niger Aa-20, Aspergillus niger AKU 3302, Aspergillus niger ATCC 9029, Aspergillus niger B, Aspergillus niger B03, Aspergillus niger B60, Aspergillus niger BCC14405, Aspergillus niger BRFM 131, Aspergillus niger CCIM, Aspergillus niger CDP-AI, Aspergillus niger CFR 1105, Aspergillus niger CS 180, Aspergillus niger CuZn-SOD, Aspergillus niger FS3, Aspergillus niger FTCC, Aspergillus niger FTCC 5003, Aspergillus niger GH1, Aspergillus niger GRM 3, Aspergillus niger HA37, Aspergillus niger I-1472, Aspergillus niger IFO31125, Aspergillus niger IFO 31628, Aspergillus niger IIIAn/8, Aspergillus niger IMI 303386, Aspergillus niger K10, Aspergillus niger KF-267, Aspergillus niger KI, Aspergillus niger LCF 8, Aspergillus niger M47, Aspergillus niger MIUG 16, Aspergillus niger MTCC 2425, Aspergillus niger MTCC F0101, Aspergillus niger N204, Aspergillus niger N400, Aspergillus niger N400(CBS 120-49), Aspergillus niger N402., Aspergillus niger N402, Aspergillus niger NCIM 1207, Aspergillus niger NCIM 548, Aspergillus niger NCIM 565, Aspergillus niger NFCCP, Aspergillus niger NIAB 280, Aspergillus niger NRRL 2270, Aspergillus niger NRRL3, Aspergillus niger NRRL-3, Aspergillus niger NRRL 3135, Aspergillus niger NRRL3135., Aspergillus niger NRRL 326, Aspergillus niger NRRL 330, Aspergillus niger NRRL337, Aspergillus niger NRRL 372, Aspergillus niger NRRL372, Aspergillus niger NRRL 4361, Aspergillus niger NRRL 65, Aspergillus niger PKL 104, Aspergillus niger R-27, Aspergillus niger RD6.13, Aspergillus niger RH5344, Aspergillus niger SA6, Aspergillus niger SL-09, Aspergillus niger T0005/007-2, Aspergillus niger T213., Aspergillus niger T213, Aspergillus niger Tiegh, Aspergillus niger UBC 814, Aspergillus niger UBC814, Aspergillus niger UV-11, Aspergillus niger ZD11--650815
hydrolysis of carboxylic esterRhodococcus sp., Rhodococcus sp. 19070, Rhodococcus sp. 400, Rhodococcus sp. 7, Rhodococcus sp. A81, Rhodococcus sp. AA21, Rhodococcus sp. AIU Z-35-1, Rhodococcus sp. AJ270, Rhodococcus sp. AK32, Rhodococcus sp. AN-22, Rhodococcus sp. ATCC39484, Rhodococcus sp. B1, Rhodococcus sp. C1, Rhodococcus sp. C9, Rhodococcus sp. C-x, Rhodococcus sp. DK17, Rhodococcus sp. DX-35, Rhodococcus sp. ECU0066, Rhodococcus sp. F2-7-3, Rhodococcus sp. Fp2, Rhodococcus sp. G-74-2, Rhodococcus sp. GK1, Rhodococcus sp. H1, Rhodococcus sp. HR199, Rhodococcus sp. IGT8, Rhodococcus sp. IGTS8, Rhodococcus sp. IOC-77, Rhodococcus sp. J1, Rhodococcus sp. M4, Rhodococcus sp. M-750, Rhodococcus sp. M-777, Rhodococcus sp. N186/21, Rhodococcus sp. N595, Rhodococcus sp. N771, Rhodococcus sp. N-774, Rhodococcus sp. NCIMB 11215, Rhodococcus sp. NCIMB 11216, Rhodococcus sp. NCIMB 12038, Rhodococcus sp. NCIMB12038, Rhodococcus sp. NCIMB 9784, Rhodococcus sp. NDB 1165, Rhodococcus sp. Novo SP361, Rhodococcus sp. P1, Rhodococcus sp. R04, Rhodococcus sp. R312, Rhodococcus sp. RHA1, Rhodococcus sp. S13-4, Rhodococcus sp. SHZ-1, Rhodococcus sp. TK6--652394
hydrolysis of carboxylic esterAlphacoronavirus, Alphacoronavirus SARS-CoV--652759
hydrolysis of carboxylic esterMeyerozyma guilliermondii, Meyerozyma guilliermondii B10-05, Meyerozyma guilliermondii FTI, Meyerozyma guilliermondii FTI 20037, Meyerozyma guilliermondii H17, Meyerozyma guilliermondii NRRL Y-17257, Meyerozyma guilliermondii OUC1, Meyerozyma guilliermondii PYCC 3012, Meyerozyma guilliermondii UFMG-Y65, Meyerozyma guilliermondii Y-1017--653087
hydrolysis of carboxylic esterCymbopogon martinii--653450
hydrolysis of carboxylic esterHypocrea jecorinaA7J2C6-695758
transacetylationCellvibrio japonicus, Cellvibrio japonicus NCIMB 10462, Clostridium thermocellum, Clostridium thermocellum 27405., Clostridium thermocellum 651, Clostridium thermocellum ATCC27405, Clostridium thermocellum CBH III, Clostridium thermocellum CelJ, Clostridium thermocellum F7, Clostridium thermocellum YM4, Clostridium thermocellum ZJL4--715029
transesterificationHypocrea jecorina, Hypocrea jecorina ALKO2877, Hypocrea jecorina CBH I, Hypocrea jecorina Cel6A, Hypocrea jecorina CL847, Hypocrea jecorina FTKO-39, Hypocrea jecorina PC-3-7, Hypocrea jecorina QM6a, Hypocrea jecorina QM 9414, Hypocrea jecorina QM9414, Hypocrea jecorina RUT C-30, Hypocrea jecorina RUT C30, Hypocrea jecorina RUT-C30, Hypocrea jecorina ZU-02--666091

PATHWAYKEGG LinkMetaCyc Link
sophorolipid degradation-SOPHOROSYLOXYDOCOSANOATE-DEG-PWY

SYSTEMATIC NAMEIUBMB Comments
acetic-ester acetylhydrolase-

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
6-O-deacetylaseCellvibrio japonicus, Cellvibrio japonicus NCIMB 10462, Clostridium thermocellum--715029
ACECellvibrio japonicus, Cellvibrio japonicus NCIMB 10462, Clostridium thermocellum--715029
acetate ester-hydrolysing esteraseSaccharomyces cerevisiae-ethyl acetate- and isoamyl acetate-hydrolysing esterase activities698520
acetate ester-hydrolysing esteraseWickerhamomyces anomalus, Wickerhamomyces anomalus AL112, Wickerhamomyces anomalus K, Wickerhamomyces anomalus NCYC 434, Wickerhamomyces anomalus SL-125--698520
acetic acid esteraseEleusine coracana--680319
Acetic ester hydrolase----
Acetyl esteraseAspergillus niger--650815
Acetyl esteraseMeyerozyma guilliermondii--653087
Acetyl esteraseHypocrea jecorinaA7J2C6-695758
Acetyl esteraseStreptomyces sp.--701417
Acetyl esteraseCellvibrio japonicus, Cellvibrio japonicus NCIMB 10462, Clostridium thermocellum--715029
Acetyl esteraseSalmonella enterica serovar Typhi Vi phage I, Salmonella enterica serovar Typhi Vi phage III, Salmonella enterica serovar Typhi Vi phage IV, Salmonella enterica serovar Typhi Vi phage V, Salmonella enterica serovar Typhi Vi phage VI, Salmonella enterica serovar Typhi Vi phage VII--715373
Acetyl esteraseEscherichia coli--701087, 715950
acetyl-esteraseEscherichia coli--682718, 682897
AEAspergillus niger--650815
AEAlphacoronavirus, Alphacoronavirus SARS-CoV--652759
AesEscherichia coli--663520, 682718, 682897, 701087
Aes1Hypocrea jecorinaA7J2C6; encoded by gene aes1, contains GDSY lipase motif695758
alpha/beta-acetylesteraseVitis vinifera--678012
BS2Bacillus subtilis--695582
C-esterase----
C20orf3Homo sapiens--696095
carbohydrate esteraseHypocrea jecorinaA7J2C6new family, less than 30% identity to carbohydrate esterases of known families695758
CE2Clostridium thermocellum--715029
CE2ACellvibrio japonicus-isozyme715029
CE2BCellvibrio japonicus-isozyme715029
CE2CCellvibrio japonicus-isozyme715029
Chloroesterase----
Citrus acetylesterase----
EST-4Vitis vinifera--678012
Est1Kluyveromyces marxianus--699069
EST2Kluyveromyces marxianus, Kluyveromyces marxianus B57, Kluyveromyces marxianus CBS 1553, Kluyveromyces marxianus CBS6556, Kluyveromyces marxianus CCEBI 2011, Kluyveromyces marxianus DSM5418, Kluyveromyces marxianus IMB3, Kluyveromyces marxianus NBIMCC 1984, Kluyveromyces marxianus NRRL Y-7571, Kluyveromyces marxianus UNSW 510700, Kluyveromyces marxianus Y-488--699069
GAECymbopogon martinii--653450
geranyl acetate cleaving esteraseCymbopogon martinii--653450
haemagglutinin-esteraseAlphacoronavirus, Alphacoronavirus SARS-CoV--652759
HEAlphacoronavirus, Alphacoronavirus SARS-CoV--652759
non-specific acetyl esteraseAspergillus oryzae--680967
p-nitrophenyl acetate esterase----
ragi acetic acid esteraseEleusine coracana--680319
HerERhodococcus sp.--652394
additional informationAspergillus oryzae-the enzyme belongs to the carbohydrate esterase family 1680967

CAS REGISTRY NUMBERCOMMENTARY
9000-82-2-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Acinetobacter sp.strain CGMCC 0789666086--Manually annotated by BRENDA team
Acinetobacter sp. CGMCCstrain CGMCC 0789666086--Manually annotated by BRENDA team
Alphacoronavirusdifferent strains of respiratory and enteropathogenic viruses, bovine, isolated from nasal swab samples and lung tissues of feedlot cattle with acute respiratory tract disease, the viruses exhibiting distinct phenotype features from enteropathogenic bovine coronaviruses652759--Manually annotated by BRENDA team
Antheraea polyphemus-35226--Manually annotated by BRENDA team
Aspergillus niger-23596, 35230, 35244, 650815--Manually annotated by BRENDA team
Aspergillus oryzae-35241, 680967--Manually annotated by BRENDA team
Bacillus pumilus-23597--Manually annotated by BRENDA team
Bacillus subtilis-35235, 695582--Manually annotated by BRENDA team
Bos taurus-35224--Manually annotated by BRENDA team
Candida bororiensis-35232--Manually annotated by BRENDA team
Cellvibrio japonicus-715029--Manually annotated by BRENDA team
Cichorium intybuscv. Nausica and Arancha665872--Manually annotated by BRENDA team
Citrus sinensis-663817--Manually annotated by BRENDA team
Clostridium thermocellum-715029--Manually annotated by BRENDA team
Cymbopogon martiniipalmarosa, 5 isozymes Est-A, Est-B, Est-C, Est-D, Est-E653450--Manually annotated by BRENDA team
Drosophila mojavensis-35228--Manually annotated by BRENDA team
Eleusine coracanafinger millet, also known as ragi, indigenous minor millet in india680319--Manually annotated by BRENDA team
Escherichia coli-664019, 682718, 701087--Manually annotated by BRENDA team
Escherichia coli-715950P23872SwissProtManually annotated by BRENDA team
Escherichia coligene ybaC682897--Manually annotated by BRENDA team
Escherichia colirecombinant protein663520--Manually annotated by BRENDA team
Fibrobacter succinogenesS8523600--Manually annotated by BRENDA team
Fibrobacter succinogenes S85S8523600--Manually annotated by BRENDA team
Homo sapiens-696095--Manually annotated by BRENDA team
Hypocrea jecorina-23605, 35223, 666091--Manually annotated by BRENDA team
Hypocrea jecorinaanamorph Trichoderma reesei, strain Rut-C30 (hypercellulolytic mutant of Trichoderma reesei QM 6a); Trichoderma reesei strain ATCC 56765695758A7J2C6SwissProtManually annotated by BRENDA team
Kluyveromyces marxianusCBS 1553699069--Manually annotated by BRENDA team
Kluyveromyces marxianus CBS 1553CBS 1553699069--Manually annotated by BRENDA team
Lactobacillus plantarum-35236--Manually annotated by BRENDA team
Marchantia polymorphatwo isoenzymes666089--Manually annotated by BRENDA team
Meyerozyma guilliermondiistrain NRRL Y-17257653087--Manually annotated by BRENDA team
Meyerozyma guilliermondii NRRL Y-17257strain NRRL Y-17257653087--Manually annotated by BRENDA team
Nocardia sp.strain LSU 16935233--Manually annotated by BRENDA team
Nocardia sp. LSU 169strain LSU 16935233--Manually annotated by BRENDA team
Orpinomyces sp.strain PC-235242--Manually annotated by BRENDA team
Penicillium purpurogenum-23594, 35238--Manually annotated by BRENDA team
Petroselinum crispumparsley35199--Manually annotated by BRENDA team
Rhodococcus sp.strain H1652394--Manually annotated by BRENDA team
Rhodococcus sp. H1strain H1652394--Manually annotated by BRENDA team
Rhodotorula mucilaginosa-23601--Manually annotated by BRENDA team
Saccharomyces cerevisiaeKyokai 701698520--Manually annotated by BRENDA team
Salmonella enterica serovar Typhi Vi phage I-715373--Manually annotated by BRENDA team
Salmonella enterica serovar Typhi Vi phage III-715373--Manually annotated by BRENDA team
Salmonella enterica serovar Typhi Vi phage IV-715373--Manually annotated by BRENDA team
Salmonella enterica serovar Typhi Vi phage V-715373--Manually annotated by BRENDA team
Salmonella enterica serovar Typhi Vi phage VI-715373--Manually annotated by BRENDA team
Salmonella enterica serovar Typhi Vi phage VII-715373--Manually annotated by BRENDA team
Schizophyllum commune-23607--Manually annotated by BRENDA team
Sclerotinia sp.-35234--Manually annotated by BRENDA team
Streptomyces lividans-35239--Manually annotated by BRENDA team
Streptomyces olivochromogenes-35193--Manually annotated by BRENDA team
Streptomyces sp.PC22701417--Manually annotated by BRENDA team
Sus scrofa-33532--Manually annotated by BRENDA team
Trypanosoma brucei-35231--Manually annotated by BRENDA team
Vitis viniferacultivars Italia, Rubi, Benitaka, and Brasil678012--Manually annotated by BRENDA team
Wickerhamomyces anomaluswild-type strain NBRC and EAL-6, a low-respiratory, petite mutant of strain NBRC698520--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
physiological functionSalmonella enterica serovar Typhi Vi phage I, Salmonella enterica serovar Typhi Vi phage III, Salmonella enterica serovar Typhi Vi phage IV, Salmonella enterica serovar Typhi Vi phage V, Salmonella enterica serovar Typhi Vi phage VI, Salmonella enterica serovar Typhi Vi phage VII-a conserved acetyl esterase domain targets bacteriophages to the Vi capsular receptor of Salmonella enterica serovar Typhi715373

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
(4R)-hydroxy-3-methyl-2-(2-propynyl)-cyclopent-2-enone acetate + H2O?
show the reaction diagram		Acinetobacter sp.-highly enantioselective enzyme, enantioselectivity raises by 1.2fold from 16 to 20 when reaction temperature is raised from 30°C to 60°C. Both enantioselectivity and activity are raised by addition of the cationic detergent benzethonium chloride666086--?
(R)-1,2-O-isopropylidene glycol acetate + H2O(R)-1,2-O-isopropylidene glycol + acetate
show the reaction diagram		Kluyveromyces marxianus--699069--?
(R,S)-1,2-O-isopropylidene glycol butyrate + H2O(R)-1,2-O-isopropylidene glycol + butyrate
show the reaction diagram		Kluyveromyces marxianus-fresh bacterial cells or purified EST1 (0.01 micromol p-nitrophenol/min), 28°C, pH 8, 5% (v/v) glycerol, 1 mM EDTA, 1 mM dithiothreitol699069low enantioselectivity (enantiomeric ratio E: 2.5 for EST1, E: 2.5 for whole cells)-?
(R,S)-1,2-O-isopropylidene glycol caproate + H2O(R)-1,2-O-isopropylidene glycol + caproate
show the reaction diagram		Kluyveromyces marxianus-fresh bacterial cells or purified EST1 (0.01 micromol p-nitrophenol/min), 28°C, pH 8, 5% (v/v) glycerol, 1 mM EDTA, 1 mM dithiothreitol699069no enantioselectivity by EST1 (enantiomeric ratio E: 1) compared to E: 2.8 for whole cells-?
1-naphthol acetate + H2O1-naphthol + acetate
show the reaction diagram		Lactobacillus plantarum--35236--?
1-naphthol acetate + H2O1-naphthol + acetate
show the reaction diagram		Aspergillus niger--35230---
1-naphthol acetate + H2O1-naphthol + acetate
show the reaction diagram		Bacillus pumilus--23597--?
1-naphthol acetate + H2O1-naphthol + acetate
show the reaction diagram		Antheraea polyphemus--35226--?
1-naphthyl acetate + H2O1-naphthol + acetate
show the reaction diagram		Meyerozyma guilliermondii--653087-653087?
1-naphthyl acetate + H2O1-naphthol + acetate
show the reaction diagram		Vitis vinifera--678012--?
1-naphthyl acetate + H2O1-naphthol + acetate
show the reaction diagram		Eleusine coracana--680319product determination by NMR and mass spectroscopy-?
1-phenyl-1-(trifluoromethyl)prop-2-yn-1-yl acetate + H2O1-phenyl-1-(trifluoromethyl)prop-2-yn-1-ol + acetate
show the reaction diagram		Bacillus subtilis-determination of enantiopreference, wild-type R enantioselectivity is 42695582spectrophotometric assay coupling released acetate to NADH generation-?
2-benzylcyclohex-1-en-1-yl acetate + H2O2-benzylcyclohexanone + acetate
show the reaction diagram		Marchantia polymorpha-isoenzyme Est I, 99% conversion of substrate with 99% S-configuration of product, isoenzyme Est II, 11% conversion of substrate with R-configuration of product666089--?
2-ethylcyclohex-1-en-1-yl acetate + H2O2-ethylcyclohexanone + acetate
show the reaction diagram		Marchantia polymorpha-isoenzyme Est I, 99% conversion of substrate with 99% S-configuration of product666089--?
2-isopropylcyclohex-1-en-1-yl acetate + H2O2-isopropylcyclohexanone + acetate
show the reaction diagram		Marchantia polymorpha-isoenzyme Est I, 10% conversion of substrate with R-configuration of product666089--?
2-methylcyclohex-1-en-1-yl acetate + H2O(S)-2-methylcyclohexanone + acetate
show the reaction diagram		Marchantia polymorpha-isoenzyme Est I, 99% conversion of substrate with 99% S-configuration of product666089--?
2-n-pentylcyclohex-1-en-1-yl acetate + H2O2-n-pentylcyclohexanone + acetate
show the reaction diagram		Marchantia polymorpha-isoenzyme Est I, 20% conversion of substrate with R-configuration of product, isoenzyme Est II, 16% conversion of substrate with S-configuration of product666089--?
2-n-propylcyclohex-1-en-1-yl acetate + H2O2-n-propylcyclohexanone + acetate
show the reaction diagram		Marchantia polymorpha-isoenzyme Est I, 99% conversion of substrate with 99% R-configuration of product666089--?
2-naphthol acetate + H2O2-naphthol + acetate
show the reaction diagram		Bacillus subtilis--35235--?
2-naphthol acetate + H2O2-naphthol + acetate
show the reaction diagram		Antheraea polyphemus--35226--?
2-naphthyl acetate + H2O2-naphthol + acetate
show the reaction diagram		Vitis vinifera--678012--?
2-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O?
show the reaction diagram		Clostridium thermocellum, Cellvibrio japonicus--715029--?
2-t-butylcyclohex-1-en-1-yl acetate + H2O2-t-butylcyclohexanone + acetate
show the reaction diagram		Marchantia polymorpha-isoenzyme Est I, 21% conversion of substrate with S-configuration of product, isoenzyme Est II, 20% conversion of substrate with R-configuration of product666089--?
3,6-diacetylmorphine + H2Omorphine + acetate
show the reaction diagram		Rhodococcus sp.-i.e. heroin, rapid spontaneous hydrolysis of the instable 3-acetyl group652394-652394?
3-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O?
show the reaction diagram		Clostridium thermocellum, Cellvibrio japonicus--715029--?
3-O-methyl D-glucopyranoside + vinyl acetate? + vinyl alcohol
show the reaction diagram		Clostridium thermocellum, Cellvibrio japonicus--715029--?
4-methylumbelliferone + H2O?
show the reaction diagram		Trypanosoma brucei--35231--?
4-methylumbelliferyl acetate + H2O?
show the reaction diagram		Trypanosoma brucei--35231---
4-methylumbelliferyl acetate + H2O?
show the reaction diagram		Bacillus pumilus--23597--?
4-methylumbelliferyl acetate + H2O?
show the reaction diagram		Schizophyllum commune--23607--?
4-nitrophenyl acetate + H2O4-nitrophenol + acetate
show the reaction diagram		Hypocrea jecorinaA7J2C6-695758--?
4-nitrophenyl acetate + H2O4-nitrophenol + acetate
show the reaction diagram		Eleusine coracana--680319product determination by NMR and mass spectroscopy-?
4-nitrophenyl acetate + H2O4-nitrophenol + acetate
show the reaction diagram		Alphacoronavirus-serine esterase activity652759-652759?
4-nitrophenyl acetate + H2O4-nitrophenol + acetate
show the reaction diagram		Hypocrea jecorinaA7J2C65 min, 30°C, pH 5.8, for kinetic analyses: 0.05-5 mM substrate in 100 mM sodium phosphate buffer695758analysis by spectrophotometry at 410 nm-?
4-nitrophenyl acetate + H2O4-nitrophenol + acetate
show the reaction diagram		Bacillus subtilis-determination of specific activity695582--?
4-nitrophenyl acetate + H2O4-nitrophenol + acetate
show the reaction diagram		Alphacoronavirus SARS-CoV-serine esterase activity652759-652759?
4-nitrophenyl butyrate + H2O4-nitrophenol + butyrate
show the reaction diagram		Escherichia coliP23872-715950--?
4-nitrophenyl butyrate + H2O4-nitrophenol + butyrate
show the reaction diagram		Hypocrea jecorinaA7J2C65 min, 30°C, pH 5.8695758--?
4-nitrophenyl formate + H2O4-nitrophenol + formate
show the reaction diagram		Hypocrea jecorinaA7J2C65 min, 30°C, pH 5.8695758--?
4-nitrophenyl hexanoate + H2O4-nitrophenol + hexanoate
show the reaction diagram		Escherichia coli--682718--?
4-nitrophenyl-2-O-acetyl-alpha-L-arabinofuranoside + H2O4-nitrophenyl-alpha-L-arabinofuranoside + acetate
show the reaction diagram		Aspergillus oryzae--680967--?
4-nitrophenyl-2-O-acetyl-beta-D-xylopyranoside + H2O4-nitrophenyl-beta-D-xylopyranoside + acetate
show the reaction diagram		Aspergillus oryzae-preferred substrate680967--?
4-nitrophenyl-3-O-acetyl-beta-D-xylopyranoside + H2O4-nitrophenyl-beta-D-xylopyranoside + acetate
show the reaction diagram		Aspergillus oryzae--680967--?
4-nitrophenyl-4-O-acetyl-beta-D-xylopyranoside + H2O4-nitrophenyl-beta-D-xylopyranoside + acetate
show the reaction diagram		Aspergillus oryzae--680967--?
4-nitrophenyl-5-O-acetyl-alpha-L-arabinofuranoside + H2O4-nitrophenyl-alpha-L-arabinofuranoside + acetate
show the reaction diagram		Aspergillus oryzae--680967--?
4-nitrophenyl-beta-D-xylopyranoside monoacetate + H2O4-nitrophenyl-beta-D-xylopyranoside + acetate
show the reaction diagram		Hypocrea jecorinaA7J2C64 mM substrate, position-specificity, hydrolysis activity is specific for 3-, and 4-O-acetyl 4-nitrophenyl-beta-D-xylopyranoside, 2-O-acetyl 4-nitrophenyl-beta-D-xylopyranoside is a bad substrate695758beta-xylosidase-coupled assay-?
4-nitrophenyl-butanoate + H2O4-nitrophenol + butanoate
show the reaction diagram		Escherichia coli--682718--?
4-nitrophenyl-D-glucopyranoside + vinyl acetate4-nitrophenyl 3-O-acetyl-D-glucopyranoside + ethylene
show the reaction diagram		Hypocrea jecorina-yield is 70.2%666091--?
4-O-acetyl 4-nitrophenyl beta-D-xylopyranoside + H2O?
show the reaction diagram		Clostridium thermocellum, Cellvibrio japonicus--715029--?
6,6-diacetyl 13-sophorosyloxysocosanoic acid + H2O?
show the reaction diagram		Candida bororiensis--35232--?
6-acetylmorphine + H2Omorphine + acetate
show the reaction diagram		Rhodococcus sp.-substrate specificity652394-652394?
6-O-acetyl-D-mannopyranose + H2O?
show the reaction diagram		Cellvibrio japonicus--715029--?
6-O-acetyl-D-mannopyranoside + H2O?
show the reaction diagram		Clostridium thermocellum--715029--?
7-aminocephalosporanic acid + H2Odeacetylated 7-aminocephalosporanic acid + acetate
show the reaction diagram		Citrus sinensis--663817--?
9-O-acetyl sialic acid + H2Osialic acid + acetate
show the reaction diagram		Alphacoronavirus, Alphacoronavirus SARS-CoV--652759-652759?
acetyl xylan + H2Oxylan + acetate
show the reaction diagram		Aspergillus niger-from beech wood or oat spelt650815-650815?
acetylxylan + H2O?
show the reaction diagram		Aspergillus niger--23596--?
acetylxylan + H2O?
show the reaction diagram		Meyerozyma guilliermondii--653087-653087?
acetylxylan + H2O?
show the reaction diagram		Bacillus pumilus--23597--?
acetylxylan + H2O?
show the reaction diagram		Streptomyces olivochromogenes--35193--?
acetylxylan + H2O?
show the reaction diagram		Rhodotorula mucilaginosa--23601--?
acetylxylan + H2O?
show the reaction diagram		Aspergillus oryzae--35241--?
acetylxylan + H2O?
show the reaction diagram		Fibrobacter succinogenes--23600--?
acetylxylan + H2O?
show the reaction diagram		Penicillium purpurogenum--23594--?
acetylxylan + H2O?
show the reaction diagram		Streptomyces lividans--35239--?
acetylxylan + H2O?
show the reaction diagram		Schizophyllum commune--23607--?
acetylxylan + H2O?
show the reaction diagram		Orpinomyces sp., Orpinomyces sp. KF7--35242--?
acetylxylan + H2Oxylan + acetate
show the reaction diagram		Streptomyces sp.-60°C, pH 6, acetyl xylan esterase (acetate-releasing) activity on birchwood xylan701417---
alpha-D-glucopyranosyl alpha-D-glucopyranoside + vinyl acetate? + vinyl alcohol
show the reaction diagram		Cellvibrio japonicus--715029--?
alpha-naphthyl acetate + H2Oalpha-naphthol + acetate
show the reaction diagram		Aspergillus oryzae--35241--?
alpha-naphthyl acetate + H2Oalpha-naphthol + acetate
show the reaction diagram		Fibrobacter succinogenes--23600--?
alpha-naphthyl acetate + H2Oalpha-naphthol + acetate
show the reaction diagram		Hypocrea jecorina--35223--?
alpha-naphthyl acetate + H2Oalpha-naphthol + acetate
show the reaction diagram		Penicillium purpurogenum--23594--?
alpha-naphthyl acetate + H2Oalpha-naphthol + acetate
show the reaction diagram		Streptomyces sp.-15 min, 50°C, pH 6.5701417--?
alpha-naphthyl acetate + H2Oalpha-naphthol + acetate
show the reaction diagram		Kluyveromyces marxianus-EST1, non-denaturating PAGE, pH7.4699069zymogram analyses using Fast Blue PR salt-?
apigenin 7-O-(6-O-malonylglucoside) + H2O?
show the reaction diagram		Petroselinum crispum--35199--?
beta-naphthyl acetate + H2Obeta-naphthol + acetate
show the reaction diagram		Cymbopogon martinii--653450-653450?
beta-naphthyl acetate + H2Obeta-naphthol + acetate
show the reaction diagram		Homo sapiens-sucrose density gradient cell fractions from Hep-G2 and RINm5F cells, pH 8696095SDS-PAGE and in gel-zymography-?
bovine submaxillary mucin + H2Odeacetylated bovine submaxillary mucin + acetate
show the reaction diagram		Alphacoronavirus, Alphacoronavirus SARS-CoV--652759-652759?
cellobiose + vinyl acetate? + vinyl alcohol
show the reaction diagram		Clostridium thermocellum, Cellvibrio japonicus--715029--?
cellobiose octaacetate + H2O?
show the reaction diagram		Aspergillus niger-low activity650815-650815?
cellulose acetate + H2Ocellulose + acetate
show the reaction diagram		Aspergillus niger-also in form of acetylated starch, water-soluble and water-insoluble substrate, specific for acetyl substituents at the C2- and C3-positions, C6-acetyl is not hydrolyzed650815-650815?
cephalosporin C + H2Odeacetylated cephalosporin C + acetate
show the reaction diagram		Citrus sinensis--663817--?
chlorogenic acid + H2O?
show the reaction diagram		Aspergillus niger--35230--?
D-galactose + vinyl acetate? + vinyl alcohol
show the reaction diagram		Cellvibrio japonicus-isozyme CE2B shows 22% transacetylation conversion, isozyme CE2A shows 48% transacetylation conversion715029--?
D-galactose + vinyl acetate? + vinyl alcohol
show the reaction diagram		Clostridium thermocellum-isozyme CE2B shows 89% transacetylation conversion715029--?
D-glucopyranosyl beta-(1,3)-xylopyranoside + vinyl acetate? + vinyl alcohol
show the reaction diagram		Clostridium thermocellum--715029--?
D-glucopyranosyl beta-(1,4)-xylopyranoside + vinyl acetate? + vinyl alcohol
show the reaction diagram		Clostridium thermocellum, Cellvibrio japonicus--715029--?
D-glucose + vinyl acetate? + vinyl alcohol
show the reaction diagram		Cellvibrio japonicus-isozyme CE2B shows 31% transacetylation conversion, isozyme CE2A shows 25% transacetylation conversion715029--?
D-glucose + vinyl acetate? + vinyl alcohol
show the reaction diagram		Clostridium thermocellum-isozyme CE2B shows 86% transacetylation conversion715029--?
D-mannose + vinyl acetate? + vinyl alcohol
show the reaction diagram		Cellvibrio japonicus-isozyme CE2B shows 46% transacetylation conversion, isozyme CE2A shows 26% transacetylation conversion715029--?
D-mannose + vinyl acetate? + vinyl alcohol
show the reaction diagram		Clostridium thermocellum-isozyme CE2B shows 71% transacetylation conversion715029--?
D-xylopyranosyl beta-(1,4)-D-glucopyranoside + vinyl acetate? + vinyl alcohol
show the reaction diagram		Clostridium thermocellum, Cellvibrio japonicus--715029--?
diacetin + H2O?
show the reaction diagram		Lactobacillus plantarum--35236--?
D_glucopyranosyl beta-(1,3)-xylopyranoside + vinyl acetate? + vinyl alcohol
show the reaction diagram		Cellvibrio japonicus--715029--?
ethyl acetate + H2Oethanol + acetate
show the reaction diagram		Nocardia sp.--35233--?
ethyl acetate + H2Oethanol + acetate
show the reaction diagram		Wickerhamomyces anomalus, Wickerhamomyces anomalus K, Wickerhamomyces anomalus NCYC 434, Wickerhamomyces anomalus SL-125, Wickerhamomyces anomalus AL112-25°C, 1 h, pH 7, 7 mM MgCl2, cell extracts698520--?
geraniol acetate + H2Ogeraniol + acetate
show the reaction diagram		Cymbopogon martinii-enzyme plays a role in geraniol production and improving palmarosa oil quality during florescence development653450-653450?
geranyl acetate + H2Ogeraniol + acetate
show the reaction diagram		Cymbopogon martinii--653450-653450?
glucose pentaacetate + H2O?
show the reaction diagram		Bacillus pumilus--23597--?
glucose pentaacetate + H2O?
show the reaction diagram		Orpinomyces sp.--35242--?
glucose pentaacetate + H2O?
show the reaction diagram		Aspergillus niger-good substrate650815-650815?
glucose pentaacetate + H2O?
show the reaction diagram		Orpinomyces sp. KF7--35242--?
glycerol triacetate + H2O?
show the reaction diagram		Lactobacillus plantarum--35236--?
glycerol triacetate + H2O?
show the reaction diagram		Aspergillus niger--35230--?
hemicellulose + H2O?
show the reaction diagram		Hypocrea jecorina--35223--?
isoamyl acetate + H2Oisoamyl alcohol + acetate
show the reaction diagram		Wickerhamomyces anomalus, Wickerhamomyces anomalus K, Wickerhamomyces anomalus NCYC 434, Wickerhamomyces anomalus SL-125, Wickerhamomyces anomalus AL112-25°C, 1 h, pH 7, 7 mM MgCl2, cell extracts698520--?
kojic acid + vinyl acetate7-O-acetyl-kojic acid + ethylene
show the reaction diagram		Hypocrea jecorina-yield is 30.9%666091--?
malonylated isorhamnetin 3-O-glucoside + H2O?
show the reaction diagram		Petroselinum crispum--35199--?
methyl 2,3-O-diacetyl-beta-D-xylopyranoside + H2O?
show the reaction diagram		Clostridium thermocellum, Cellvibrio japonicus--715029--?
methyl 2,4-O-diacetyl-beta-D-xylopyranoside + H2O?
show the reaction diagram		Clostridium thermocellum, Cellvibrio japonicus--715029--?
methyl 2-O-methyl-beta-D-glucopyranoside + vinyl acetate? + vinyl alcohol
show the reaction diagram		Clostridium thermocellum, Cellvibrio japonicus--715029--?
methyl 3,4-O-diacetyl-beta-D-xylopyranoside + H2O?
show the reaction diagram		Clostridium thermocellum, Cellvibrio japonicus--715029--?
methyl 4-O-methyl-alpha-D-glucopyranoside + vinyl acetate? + vinyl alcohol
show the reaction diagram		Clostridium thermocellum, Cellvibrio japonicus--715029--?
methyl 6-O-acetyl-beta-D-glucopyranoside + H2O?
show the reaction diagram		Clostridium thermocellum, Cellvibrio japonicus--715029--?
methyl acetate + H2Omethanol + acetate
show the reaction diagram		Nocardia sp.--35233--?
methyl alpha-D-glucopyranoside + vinyl acetate? + vinyl alcohol
show the reaction diagram		Clostridium thermocellum, Cellvibrio japonicus--715029--?
methyl beta-D-glucopyranoside + vinyl acetate? + vinyl alcohol
show the reaction diagram		Clostridium thermocellum, Cellvibrio japonicus--715029--?
methyl-beta-D-xylopyranoside + vinylacetatemethyl-beta-D-xylopyranoside monoacetate + vinyl alcohol
show the reaction diagram		Hypocrea jecorinaA7J2C6transacetylation of methylxyloside in aqueous solution (40°C, pH 6, 25 mM methyl-beta-D-xylopyranoside)695758analysis by thin-layer chromatography-?
methyl-D-glucopyranoside + vinyl acetatemethyl 3-O-acetyl-D-glucopyranoside + ethylene
show the reaction diagram		Hypocrea jecorina-yield is 56.4%666091--?
naphthyl acetate + H2Onaphthol + acetate
show the reaction diagram		Drosophila mojavensis--35228--?
naphthyl butyrate + H2Onaphthol + butyrate
show the reaction diagram		Drosophila mojavensis--35228--?
naphthyl propionate + H2Onaphthol + propionate
show the reaction diagram		Drosophila mojavensis--35228--?
O-acetyl-1,4-beta-xylooligosaccharide + H2O1,4-beta-xylooligosaccharide + acetic acid
show the reaction diagram		Streptomyces olivochromogenes--35193--ir
O-acetyl-1,4-beta-xylooligosaccharide + H2O1,4-beta-xylooligosaccharide + acetic acid
show the reaction diagram		Schizophyllum commune--23607-23607ir
O-acetyl-4-O-methyl-D-glucurono-D-xylan + H2O4-O-methyl-D-glucurono-D-xylan + acetic acid
show the reaction diagram		Aspergillus oryzae--35241--?
O-acetyl-4-O-methyl-D-glucurono-D-xylan + H2O4-O-methyl-D-glucurono-D-xylan + acetic acid
show the reaction diagram		Schizophyllum commune--23607-23607ir
O-acetyl-galactoglucomannan + H2O?
show the reaction diagram		Aspergillus oryzae--35241--?
p-nitrophenyl acetate + H2Op-nitrophenol + acetate
show the reaction diagram		Bos taurus--35224--?
p-nitrophenyl acetate + H2Op-nitrophenol + acetate
show the reaction diagram		Aspergillus niger--35244---
p-nitrophenyl acetate + H2Op-nitrophenol + acetate
show the reaction diagram		Aspergillus niger--23596--?
p-nitrophenyl acetate + H2Op-nitrophenol + acetate
show the reaction diagram		Bacillus pumilus--23597--?
p-nitrophenyl acetate + H2Op-nitrophenol + acetate
show the reaction diagram		Nocardia sp.--35233--?
p-nitrophenyl acetate + H2Op-nitrophenol + acetate
show the reaction diagram		Acinetobacter sp.--666086--?
p-nitrophenyl acetate + H2Op-nitrophenol + acetate
show the reaction diagram		Streptomyces lividans--35239--?
p-nitrophenyl acetate + H2Op-nitrophenol + acetate
show the reaction diagram		Orpinomyces sp.--35242--?
p-nitrophenyl acetate + H2Op-nitrophenol + acetate
show the reaction diagram		Candida bororiensis--35232--?
p-nitrophenyl acetate + H2Op-nitrophenol + acetate
show the reaction diagram		Streptomyces sp.-10 min, 50°C, pH 6.5701417--?
p-nitrophenyl acetate + H2Op-nitrophenol + acetate
show the reaction diagram		Kluyveromyces marxianus-EST1, pH 7.4, 20°C, 0.5% (w/v) Triton X-100, 0.125% (w/v) Arabic gum699069spectrophotometry, 400 nm-?
p-nitrophenyl acetate + H2Op-nitrophenol + acetate
show the reaction diagram		Orpinomyces sp. KF7--35242--?
p-nitrophenyl butyrate + H2Op-nitrophenol + butyrate
show the reaction diagram		Acinetobacter sp.--666086--?
p-nitrophenyl decanoate + H2Op-nitrophenol + decanoate
show the reaction diagram		Acinetobacter sp.--666086--?
p-nitrophenyl hexanoate + H2Op-nitrophenol + hexanoate
show the reaction diagram		Acinetobacter sp.--666086--?
p-nitrophenyl laurate + H2Op-nitrophenol + laurate
show the reaction diagram		Acinetobacter sp.--666086--?
p-nitrophenyl propionate + H2Op-nitrophenol + propionate
show the reaction diagram		Acinetobacter sp.--666086--?
penta-O-acetyl-alpha-D-glucose + H2O?
show the reaction diagram		Citrus sinensis-complete and regioselective removal of primary acetyl-group in 6-position663817--?
phenyl acetate + H2Ophenol + acetate
show the reaction diagram		Rhodococcus sp.--652394-652394?
phenyl acetate + H2Ophenol + acetate
show the reaction diagram		Homo sapiens-sucrose density gradient cell fractions from Hep-G2 and RINm5F cells, pH 8, 3 min, 1 mM CaCl2 or 5 mM EDTA696095spectrophotometry, absorbance at 270 nm-?
triacetin + H2O?
show the reaction diagram		Kluyveromyces marxianus-EST1, pH 8, 20°C, 0.5% (w/v) Triton X-100, 0.125% (w/v) Arabic gum699069titration assay-?
triacetine + H2O?
show the reaction diagram		Cichorium intybus--665872--?
tributyrin + H2O?
show the reaction diagram		Escherichia coliP23872-715950--?
tributyrin + H2O?
show the reaction diagram		Kluyveromyces marxianus-EST1, pH 8, 20°C, 0.5% (w/v) Triton X-100, 0.125% (w/v) Arabic gum699069titration assay-?
tripropionin + H2O?
show the reaction diagram		Lactobacillus plantarum--35236--?
xylobiose + vinyl acetate? + vinyl alcohol
show the reaction diagram		Clostridium thermocellum, Cellvibrio japonicus--715029--?
xylose tetraacetate + H2O?
show the reaction diagram		Bacillus pumilus--23597--?
xylose tetraacetate + H2O?
show the reaction diagram		Orpinomyces sp., Orpinomyces sp. KF7--35242--?
[(4S)-2,2-dimethyl-1,3-dioxolan-4-yl]methyl acetate + H2O[(4R)-2,2-dimethyl-1,3-dioxolan-4-yl]methanol + acetate
show the reaction diagram		Kluyveromyces marxianus-fresh bacterial cells or purified enzyme (EST1 or EST2, 0.01 micromol p-nitrophenol/min), 20°C, pH 8, 5% (v/v) glycerol, 1 mM EDTA, 1 mM dithiothreitol699069high enantioselectivity (enantiomeric ratio E: 20 for EST1, E: 15 for whole cells), thin-layer chromatography, enantiomeric composition of reactants controlled by gas chromatography, acetate detection spectrophotometrically through NADH absorbance at 340 nm-?
monoacetin + H2Oglycerol + acetate
show the reaction diagram		Lactobacillus plantarum--35236--?
additional information?-Aspergillus niger-substrate specificity and regioselectivity, deacetylation activity of the enzyme is not restricted to polymers with beta-1,4 glycosidic bonds, no activity with cellulose sulfate, the enzyme enhances endoglucanase, but is itself not influenced by endoglucanase650815-650815?
additional information?-Alphacoronavirus-enzyme has receptor-destroying enzyme function652759-652759?
additional information?-Citrus sinensis-removal of acetyl group in 3-position of beta-lactams with 98% conversion and 91-93% product yield663817---
additional information?-Vitis vinifera-acetylesterases generally prefer aliphatic substrates involving acetic acid678012---
additional information?-Eleusine coracana-the enzyme deacetylates cereal products like water soluble polysaccharides such as ragi, wheat, larch wood xylan, and gum karaya, the enzyme cleaves the acetyl groups substituted at O-2/O-3 of the xylan backbone of arabinoxylans and is known to modulate their functional properties680319---
additional information?-Aspergillus oryzae-the enzyme, feruloyl esterase AoFae, behaves as a non-specific acetyl esterase rather than a feruloyl esterase, with a preference for 2-O-acetyl-beta-D-xylopyranoside, AoFae shows only a very weak activity on feruloyl esters680967---
additional information?-Hypocrea jecorinaA7J2C6new family of microbial esterases with hemicellulase activity for biodegradation of lignocellulose, complementary activity to acetylxylan esterase Axe1695758---
additional information?-Bacillus subtilis-1-(4-fluorophenyl)-1-(trifluoromethyl)prop-2-yn-1-yl acetate, 1-(4-chlorophenyl)-1-(trifluoromethyl)prop-2-yn-1-yl acetate, 1-(4-methylphenyl)-1-(trifluoromethyl)prop-2-yn-1-yl acetate, 1-(3-fluorophenyl)-1-(trifluoromethyl)prop-2-yn-1-yl acetate, 1-(3,4-difluorophenyl)-1-(trifluoromethyl)prop-2-yn-1-yl acetate are substrates of double mutant E188W/M193C, conversion with inversed enantioselectivity, 1-phenyl-1-(trifluoromethyl)but-2-yn-1-yl acetate and 1-phenyl-1-(trifluoromethyl)prop-2-en-1-yl acetate are no substrates of neither wild-type nor any mutant695582---
additional information?-Escherichia coli-activated Sepharose CL6B-bound activity, pH 8701087---
additional information?-Kluyveromyces marxianus-activity of EST1 only on short chain triglyceride substrates, EST2 shows lower activity and enantioselectivity towards (R,S)-1,2-O-isopropylidene glycol acetate as substrate than EST1699069---
additional information?-Wickerhamomyces anomalus-isoamyl acetate and ethyl acetate-synthesizing esterase activities in mutant strain 100fold less than respective acetate ester-hydrolysing esterase activities698520---
additional information?-Kluyveromyces marxianus-no activity of EST1 on p-nitrophenyl butyrate, p-nitrophenyl caproate, p-nitrophenyl caprylate, p-nitrophenyl laurate and p-nitrophenyl palmitate, no activity of EST1 on triacylglyceride esters tricaprylin and triolein699069---
additional information?-Streptomyces sp.-no activity on p-nitrophenyl-beta-D-xylopyranoside and p-nitrophenyl-alpha-L-arabinofuranoside and no xylanolytic activity701417---
additional information?-Bacillus subtilis-size of residue at position 188 (from Asp to Trp) determines enantioselectivity and -preference695582---
additional information?-Clostridium thermocellum, Cellvibrio japonicus-no transacetylation occurs with vinyl acetate and D-xylose, xylobiose, methyl 6-O-methyl-alpha-D-glucopyranoside, 6-O-methyl D-glucopyranose, and methyl beta-D-xylopyranoside715029---
additional information?-Clostridium thermocellum, Cellvibrio japonicus-the enzyme shows a strong preference for deacetylation of the 6-position in aldohexoses. Xylose and xylooligosaccharides do not serve as acetyl group acceptors715029---
additional information?-Wickerhamomyces anomalus K, Wickerhamomyces anomalus NCYC 434, Wickerhamomyces anomalus SL-125-isoamyl acetate and ethyl acetate-synthesizing esterase activities in mutant strain 100fold less than respective acetate ester-hydrolysing esterase activities698520---
additional information?-Alphacoronavirus SARS-CoV-enzyme has receptor-destroying enzyme function652759-652759?
additional information?-Wickerhamomyces anomalus AL112-isoamyl acetate and ethyl acetate-synthesizing esterase activities in mutant strain 100fold less than respective acetate ester-hydrolysing esterase activities698520---

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
geraniol acetate + H2Ogeraniol + acetate
show the reaction diagram		Cymbopogon martinii-enzyme plays a role in geraniol production and improving palmarosa oil quality during florescence development653450-653450
additional information?-Alphacoronavirus-enzyme has receptor-destroying enzyme function652759-652759
additional information?-Hypocrea jecorinaA7J2C6new family of microbial esterases with hemicellulase activity for biodegradation of lignocellulose, complementary activity to acetylxylan esterase Axe1695758--
additional information?-Alphacoronavirus SARS-CoV-enzyme has receptor-destroying enzyme function652759-652759

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
No entries in this field

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
Ag+Acinetobacter sp., Acinetobacter sp. 15150, Acinetobacter sp. 210A, Acinetobacter sp. 3B-1, Acinetobacter sp. 4B, Acinetobacter sp. AG, Acinetobacter sp. AK226, Acinetobacter sp. C-17, Acinetobacter sp. C1W, Acinetobacter sp. CGMCC, Acinetobacter sp. CHC, Acinetobacter sp. CHE4-1, Acinetobacter sp. CR9, Acinetobacter sp. CRH-104D, Acinetobacter sp. DV1, Acinetobacter sp. HO1-N, Acinetobacter sp. JCI, Acinetobacter sp. M-1, Acinetobacter sp. M-30, Acinetobacter sp. MTCC 6816, Acinetobacter sp. NCIB, Acinetobacter sp. NCIMB 9871, Acinetobacter sp. SA1, Acinetobacter sp. ST-1, Acinetobacter sp. TD63, Acinetobacter sp. YAA-stimulation666086
Ba2+Acinetobacter sp., Acinetobacter sp. 15150, Acinetobacter sp. 210A, Acinetobacter sp. 3B-1, Acinetobacter sp. 4B, Acinetobacter sp. AG, Acinetobacter sp. AK226, Acinetobacter sp. C-17, Acinetobacter sp. C1W, Acinetobacter sp. CGMCC, Acinetobacter sp. CHC, Acinetobacter sp. CHE4-1, Acinetobacter sp. CR9, Acinetobacter sp. CRH-104D, Acinetobacter sp. DV1, Acinetobacter sp. HO1-N, Acinetobacter sp. JCI, Acinetobacter sp. M-1, Acinetobacter sp. M-30, Acinetobacter sp. MTCC 6816, Acinetobacter sp. NCIB, Acinetobacter sp. NCIMB 9871, Acinetobacter sp. SA1, Acinetobacter sp. ST-1, Acinetobacter sp. TD63, Acinetobacter sp. YAA-stimulation666086
Ca2+Acinetobacter sp.-up to 150% stimulation666086
Co2+Acinetobacter sp.-stimulation666086
Cu+Eleusine coracana-activates680319
Cu2+Eleusine coracana-activates680319
Fe3+Eleusine coracana-activates680319
Hg2+Eleusine coracana-activates680319
Mg2+Acinetobacter sp.-stimulation666086
Pb2+Acinetobacter sp.-stimulation666086
Mn2+Acinetobacter sp.-stimulation666086
additional informationStreptomyces sp.-10 mM Ca2+, Co2+, Mg2+ or Mn2+ without any affect on acetyl esterase activity701417

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
2-butanolNocardia sp., Nocardia sp. 108, Nocardia sp. 239, Nocardia sp. 3CS, Nocardia sp. LSU 169, Nocardia sp. M117, Nocardia sp. NRRL 5646, Nocardia sp. P18.3--35233 2D-image
Al3+Eleusine coracana--680319 2D-image
Ba2+Eleusine coracana--680319 2D-image
Ca2+Eleusine coracana--680319 2D-image
Ca2+Kluyveromyces marxianus-10 mM, 92% residual activity, EST1, p-nitrophenyl acetate as substrate699069 2D-image
Co2+Eleusine coracana--680319 2D-image
Co2+Kluyveromyces marxianus-10 mM, 56% residual activity, EST1, p-nitrophenyl acetate as substrate699069 2D-image
Cu2+Bacillus pumilus--23597 2D-image
Cu2+Acinetobacter sp.--666086 2D-image
Cu2+Kluyveromyces marxianus-complete inhibition, 10 mM, EST1, p-nitrophenyl acetate as substrate699069 2D-image
Cu2+Streptomyces sp.-acetyl esterase activity701417 2D-image
D-xyloseHypocrea jecorinaA7J2C6at low concentrations of 1-5 mM, altered activity with 4-nitrophenyl acetate as substrate695758 2D-image
diisopropyl fluorophosphateCandida bororiensis--35232 2D-image
dimethylarsinic acidRhodococcus sp., Rhodococcus sp. 19070, Rhodococcus sp. 400, Rhodococcus sp. 7, Rhodococcus sp. A81, Rhodococcus sp. AA21, Rhodococcus sp. AIU Z-35-1, Rhodococcus sp. AJ270, Rhodococcus sp. AK32, Rhodococcus sp. AN-22, Rhodococcus sp. ATCC39484, Rhodococcus sp. B1, Rhodococcus sp. C1, Rhodococcus sp. C9, Rhodococcus sp. C-x, Rhodococcus sp. DK17, Rhodococcus sp. DX-35, Rhodococcus sp. ECU0066, Rhodococcus sp. F2-7-3, Rhodococcus sp. Fp2, Rhodococcus sp. G-74-2, Rhodococcus sp. GK1, Rhodococcus sp. H1, Rhodococcus sp. HR199, Rhodococcus sp. IGT8, Rhodococcus sp. IGTS8, Rhodococcus sp. IOC-77, Rhodococcus sp. J1, Rhodococcus sp. M4, Rhodococcus sp. M-750, Rhodococcus sp. M-777, Rhodococcus sp. N186/21, Rhodococcus sp. N595, Rhodococcus sp. N771, Rhodococcus sp. N-774, Rhodococcus sp. NCIMB 11215, Rhodococcus sp. NCIMB 11216, Rhodococcus sp. NCIMB 12038, Rhodococcus sp. NCIMB12038, Rhodococcus sp. NCIMB 9784, Rhodococcus sp. NDB 1165, Rhodococcus sp. Novo SP361, Rhodococcus sp. P1, Rhodococcus sp. R04, Rhodococcus sp. R312, Rhodococcus sp. RHA1, Rhodococcus sp. S13-4, Rhodococcus sp. SHZ-1, Rhodococcus sp. TK6-binding mechanism652394 2D-image
EDTAHomo sapiens-metal-chelating reduces enzymatic activity by ca. 33%696095 2D-image
EDTAKluyveromyces marxianus-strong inhibitor, 10 mM, 18% residual activity, EST1, p-nitrophenyl acetate as substrate699069 2D-image
eserineEleusine coracana-competitive, complete inhibition at 25 mM680319 2D-image
Fe2+Bacillus pumilus--23597 2D-image
Fe2+Kluyveromyces marxianus-0.1 mM, 70% residual activity, EST1, p-nitrophenyl acetate as substrate699069 2D-image
Fe3+Acinetobacter sp.--666086 2D-image
Fe3+Kluyveromyces marxianus-0.1 mM, 93% residual activity, EST1, p-nitrophenyl acetate as substrate699069 2D-image
Hg2+Kluyveromyces marxianus-strong inhibitor, 10 mM, 9% residual activity, EST1, p-nitrophenyl acetate as substrate699069 2D-image
Hg2+Streptomyces sp.-strong inhibitor, 0.1 mM, acetyl esterase activity701417 2D-image
K+Kluyveromyces marxianus-10 mM, 70% residual activity, EST1, p-nitrophenyl acetate as substrate699069 2D-image
Mg2+Eleusine coracana--680319 2D-image
Mg2+Kluyveromyces marxianus-10 mM, 80% residual activity, EST1, p-nitrophenyl acetate as substrate699069 2D-image
Mn2+Eleusine coracana--680319 2D-image
N-ethylmaleimideCandida bororiensis--35232 2D-image
Ni2+Eleusine coracana--680319 2D-image
O-Ethyl-S-phenyl phosphoramidothiolateAntheraea polyphemus--35226 2D-image
p-hydroxymercuribenzoateCandida bororiensis--35232 2D-image
Pb(NO3)2Aspergillus niger--23596 2D-image
PCMBEleusine coracana-complete inhibition at 25 mM680319 2D-image
phenyl methylsulfonyl fluorideStreptomyces sp.-10 mM, 50% decreased acetyl esterase activity701417 2D-image
Primary alcoholsNocardia sp., Nocardia sp. 108, Nocardia sp. 239, Nocardia sp. 3CS, Nocardia sp. LSU 169, Nocardia sp. M117, Nocardia sp. NRRL 5646, Nocardia sp. P18.3--35233 2D-image
SDSKluyveromyces marxianus-complete inhibition, 1% (w/v), short-term incubation, EST1, p-nitrophenyl acetate as substrate699069 2D-image
Sn2+Kluyveromyces marxianus-0.1 mM, 93% residual activity, EST1, p-nitrophenyl acetate as substrate699069 2D-image
Sodium acetateHypocrea jecorinaA7J2C6at concentrations higher than 100 mM695758 2D-image
TrifluorotetradecanoneAntheraea polyphemus--35226 2D-image
Zn2+Bacillus pumilus--23597 2D-image
Zn2+Eleusine coracana--680319 2D-image
Zn2+Kluyveromyces marxianus-complete inhibition, 10 mM, EST1, p-nitrophenyl acetate as substrate699069 2D-image
Zn2+Streptomyces sp.-acetyl esterase activity701417 2D-image
Mn2+Kluyveromyces marxianus-10 mM, 60% residual activity, EST1, p-nitrophenyl acetate as substrate699069 2D-image
additional informationVitis vinifera-no inhibition by 4-chloromercuribenzoate, thiamethoxam, malathion and methyl-parathion678012-
additional informationEleusine coracana-no inhibition by iodoacetamide680319-
additional informationHypocrea jecorinaA7J2C6rates of hydrolysis of 4-nitrophenyl acetate by the purified Aes1 are inhibited by low concentrations (1.0 to 5.0 mM) but significantly enhanced by high concentrations (10.0 to 500 mM) of D-xylose695758-
additional informationStreptomyces sp.-10 mM ethylmethylaminopropyl carbodiimide or iodacetamide without effect on acetyl esterase activity701417-

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
benzethonium chlorideAcinetobacter sp., Acinetobacter sp. 15150, Acinetobacter sp. 210A, Acinetobacter sp. 3B-1, Acinetobacter sp. 4B, Acinetobacter sp. AG, Acinetobacter sp. AK226, Acinetobacter sp. C-17, Acinetobacter sp. C1W, Acinetobacter sp. CGMCC, Acinetobacter sp. CHC, Acinetobacter sp. CHE4-1, Acinetobacter sp. CR9, Acinetobacter sp. CRH-104D, Acinetobacter sp. DV1, Acinetobacter sp. HO1-N, Acinetobacter sp. JCI, Acinetobacter sp. M-1, Acinetobacter sp. M-30, Acinetobacter sp. MTCC 6816, Acinetobacter sp. NCIB, Acinetobacter sp. NCIMB 9871, Acinetobacter sp. SA1, Acinetobacter sp. ST-1, Acinetobacter sp. TD63, Acinetobacter sp. YAA-i.e. (diisobutyl phenoxyethoxyethyl) dimethyl benzylammonium chloride, up to 2.5fold enhancement of activity666086 2D-image
bis(2-ethylhexyl) sodium sulfosuccinateAcinetobacter sp., Acinetobacter sp. 15150, Acinetobacter sp. 210A, Acinetobacter sp. 3B-1, Acinetobacter sp. 4B, Acinetobacter sp. AG, Acinetobacter sp. AK226, Acinetobacter sp. C-17, Acinetobacter sp. C1W, Acinetobacter sp. CGMCC, Acinetobacter sp. CHC, Acinetobacter sp. CHE4-1, Acinetobacter sp. CR9, Acinetobacter sp. CRH-104D, Acinetobacter sp. DV1, Acinetobacter sp. HO1-N, Acinetobacter sp. JCI, Acinetobacter sp. M-1, Acinetobacter sp. M-30, Acinetobacter sp. MTCC 6816, Acinetobacter sp. NCIB, Acinetobacter sp. NCIMB 9871, Acinetobacter sp. SA1, Acinetobacter sp. ST-1, Acinetobacter sp. TD63, Acinetobacter sp. YAA-138% of initial activity666086 2D-image
bis(octadecyl) ammonium chlorideAcinetobacter sp., Acinetobacter sp. 15150, Acinetobacter sp. 210A, Acinetobacter sp. 3B-1, Acinetobacter sp. 4B, Acinetobacter sp. AG, Acinetobacter sp. AK226, Acinetobacter sp. C-17, Acinetobacter sp. C1W, Acinetobacter sp. CGMCC, Acinetobacter sp. CHC, Acinetobacter sp. CHE4-1, Acinetobacter sp. CR9, Acinetobacter sp. CRH-104D, Acinetobacter sp. DV1, Acinetobacter sp. HO1-N, Acinetobacter sp. JCI, Acinetobacter sp. M-1, Acinetobacter sp. M-30, Acinetobacter sp. MTCC 6816, Acinetobacter sp. NCIB, Acinetobacter sp. NCIMB 9871, Acinetobacter sp. SA1, Acinetobacter sp. ST-1, Acinetobacter sp. TD63, Acinetobacter sp. YAA-246% of initial activity666086 2D-image
cellobioseHypocrea jecorinaA7J2C6enhanced activity with 4-nitrophenyl acetate as substrate695758 2D-image
cetyltrimethyl ammonium bromideAcinetobacter sp., Acinetobacter sp. 15150, Acinetobacter sp. 210A, Acinetobacter sp. 3B-1, Acinetobacter sp. 4B, Acinetobacter sp. AG, Acinetobacter sp. AK226, Acinetobacter sp. C-17, Acinetobacter sp. C1W, Acinetobacter sp. CGMCC, Acinetobacter sp. CHC, Acinetobacter sp. CHE4-1, Acinetobacter sp. CR9, Acinetobacter sp. CRH-104D, Acinetobacter sp. DV1, Acinetobacter sp. HO1-N, Acinetobacter sp. JCI, Acinetobacter sp. M-1, Acinetobacter sp. M-30, Acinetobacter sp. MTCC 6816, Acinetobacter sp. NCIB, Acinetobacter sp. NCIMB 9871, Acinetobacter sp. SA1, Acinetobacter sp. ST-1, Acinetobacter sp. TD63, Acinetobacter sp. YAA-184% of initial activity666086 2D-image
Citric acidEleusine coracana--680319 2D-image
D-galactoseHypocrea jecorinaA7J2C6enhanced activity with 4-nitrophenyl acetate as substrate695758 2D-image
D-glucoseHypocrea jecorinaA7J2C61% (w/v), enhanced activity with 4-nitrophenyl acetate as substrate695758 2D-image
D-xyloseHypocrea jecorinaA7J2C6at high concentrations of 10-500 mM, enhanced activity with 4-nitrophenyl acetate as substrate695758 2D-image
xylooligosaccharideHypocrea jecorinaA7J2C650 mM, enhanced activity with 4-nitrophenyl acetate as substrate695758 2D-image
EDTAEleusine coracana--680319 2D-image
additional informationHypocrea jecorinaA7J2C6no influence on activity on 4-nitrophenyl acetate as substrate by L-arabinose, mannose, lactose; rates of hydrolysis of 4-nitrophenyl acetate by the purified Aes1 are inhibited by low concentrations (1.0 to 5.0 mM) but significantly enhanced by high concentrations (10.0 to 500 mM) of D-xylose; sodium acetate at concentrations below 100 mM does not inhibit the hydrolysis, higher concentrations inhibit the reactions; using 4-nitrophenyl acetate as substrate the activity of the recombinant enzyme is enhanced by D-xylose, D-glucose, cellobiose, D-galactose, and xylooligosaccharides but not by arabinose, mannose, or lactose695758-

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.07-(R)-1,2-O-isopropylidene glycol acetateKluyveromyces marxianus, Kluyveromyces marxianus B57, Kluyveromyces marxianus CBS 1553, Kluyveromyces marxianus CBS6556, Kluyveromyces marxianus CCEBI 2011, Kluyveromyces marxianus DSM5418, Kluyveromyces marxianus IMB3, Kluyveromyces marxianus NBIMCC 1984, Kluyveromyces marxianus NRRL Y-7571, Kluyveromyces marxianus UNSW 510700, Kluyveromyces marxianus Y-488-EST1, after preparative electrophoresis699069 2D-image
2.63-1-acetoxynaphthaleneMeyerozyma guilliermondii, Meyerozyma guilliermondii B10-05, Meyerozyma guilliermondii FTI, Meyerozyma guilliermondii FTI 20037, Meyerozyma guilliermondii H17, Meyerozyma guilliermondii NRRL Y-17257, Meyerozyma guilliermondii OUC1, Meyerozyma guilliermondii PYCC 3012, Meyerozyma guilliermondii UFMG-Y65, Meyerozyma guilliermondii Y-1017-pH 7.8, 45°C653087 2D-image
1.54-1-naphthol acetateBacillus pumilus, Bacillus pumilus 76, Bacillus pumilus A8, Bacillus pumilus B26, Bacillus pumilus BK2, Bacillus pumilus Bn-262, Bacillus pumilus CECT 5072, Bacillus pumilus DKS1, Bacillus pumilus HL721, Bacillus pumilus IPO, Bacillus pumilus P9, Bacillus pumilus PS213, Bacillus pumilus PS214--23597 2D-image
0.06-2-benzylcyclohex-1-en-1-yl acetateMarchantia polymorpha-isoenzyme Est II, pH 7.0, 35°C666089 2D-image
0.97-2-benzylcyclohex-1-en-1-yl acetateMarchantia polymorpha-isoenzyme Est I, pH 7.0, 35°C666089 2D-image
0.11-2-ethylcyclohex-1-en-1-yl acetateMarchantia polymorpha-isoenzyme Est I, pH 7.0, 35°C666089 2D-image
1.87-2-isopropylcyclohex-1-en-1-yl acetateMarchantia polymorpha-isoenzyme Est I, pH 7.0, 35°C666089 2D-image
0.09-2-methylcyclohex-1-en-1-yl acetateMarchantia polymorpha-isoenzyme Est I, pH 7.0, 35°C666089 2D-image
0.3-2-n-pentylcyclohex-1-en-1-yl acetateMarchantia polymorpha-isoenzyme Est II, pH 7.0, 35°C666089 2D-image
0.72-2-n-pentylcyclohex-1-en-1-yl acetateMarchantia polymorpha-isoenzyme Est I, pH 7.0, 35°C666089 2D-image
0.54-2-n-propylcyclohex-1-en-1-yl acetateMarchantia polymorpha-isoenzyme Est I, pH 7.0, 35°C666089 2D-image
4.42-2-O-acetyl 4-nitrophenyl beta-D-xylopyranosideCellvibrio japonicus-isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C715029 2D-image
2.01-2-t-butylcyclohex-1-en-1-yl acetateMarchantia polymorpha-isoenzyme Est II, pH 7.0, 35°C666089 2D-image
2.54-2-t-butylcyclohex-1-en-1-yl acetateMarchantia polymorpha-isoenzyme Est I, pH 7.0, 35°C666089 2D-image
1.55-3-O-acetyl 4-nitrophenyl beta-D-xylopyranosideCellvibrio japonicus-isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C715029-
0.23-4-nitrophenyl acetateHypocrea jecorinaA7J2C630°C, pH 5.8; purified recombinant enzyme, 30°C, pH 5.8695758 2D-image
0.13-4-nitrophenyl butyrateEscherichia coliP23872mutant enzyme R48E, in 20 mM phosphate buffer (pH 7.1), at 30°C715950 2D-image
0.14-4-nitrophenyl butyrateEscherichia coliP23872mutant enzyme R48K, in 20 mM phosphate buffer (pH 7.1), at 30°C715950 2D-image
0.16-4-nitrophenyl butyrateEscherichia coliP23872wild type enzyme, in 20 mM phosphate buffer (pH 7.1), at 30°C715950 2D-image
0.18-4-nitrophenyl butyrateEscherichia coliP23872mutant enzyme R48A, in 20 mM phosphate buffer (pH 7.1), at 30°C715950 2D-image
0.19-4-nitrophenyl butyrateEscherichia coliP23872mutant enzyme R48S, in 20 mM phosphate buffer (pH 7.1), at 30°C715950 2D-image
0.245-4-nitrophenyl hexanoateEscherichia coli-pH 7.1, 65°C, recombinant wild-type enzyme682718 2D-image
0.355-4-nitrophenyl hexanoateEscherichia coli-pH 7.1, 65°C, recombinant mutant T74A682718 2D-image
44.6-4-nitrophenyl-2-O-acetyl-alpha-L-arabinofuranosideAspergillus oryzae-pH 5.5, 45°C680967 2D-image
3.28-4-nitrophenyl-2-O-acetyl-beta-D-xylopyranosideAspergillus oryzae-pH 5.5, 45°C680967 2D-image
9.44-4-nitrophenyl-3-O-acetyl-beta-D-xylopyranosideAspergillus oryzae-pH 5.5, 45°C680967 2D-image
6.78-4-nitrophenyl-4-O-acetyl-beta-D-xylopyranosideAspergillus oryzae-pH 5.5, 45°C680967 2D-image
16.4-4-nitrophenyl-5-O-acetyl-alpha-L-arabinofuranosideAspergillus oryzae-pH 5.5, 45°C680967 2D-image
0.75-4-nitrophenyl-butanoateEscherichia coli-pH 7.1, 25°C, recombinant wild-type enzyme682718 2D-image
1.5-4-nitrophenyl-butanoateEscherichia coli-pH 7.1, 65°C, recombinant wild-type enzyme682718 2D-image
2-4-nitrophenyl-butanoateEscherichia coli-pH 7.1, 65°C, recombinant mutant T74A682718 2D-image
1.24-4-O-acetyl 4-nitrophenyl beta-D-xylopyranosideCellvibrio japonicus-isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C715029-
2.46-4-O-acetyl 4-nitrophenyl beta-D-xylopyranosideClostridium thermocellum-isozyme CE2, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C715029-
4.31-4-O-acetyl 4-nitrophenyl beta-D-xylopyranosideCellvibrio japonicus-isozyme CE2C, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C715029-
0.5-6-acetylmorphineRhodococcus sp., Rhodococcus sp. 19070, Rhodococcus sp. 400, Rhodococcus sp. 7, Rhodococcus sp. A81, Rhodococcus sp. AA21, Rhodococcus sp. AIU Z-35-1, Rhodococcus sp. AJ270, Rhodococcus sp. AK32, Rhodococcus sp. AN-22, Rhodococcus sp. ATCC39484, Rhodococcus sp. B1, Rhodococcus sp. C1, Rhodococcus sp. C9, Rhodococcus sp. C-x, Rhodococcus sp. DK17, Rhodococcus sp. DX-35, Rhodococcus sp. ECU0066, Rhodococcus sp. F2-7-3, Rhodococcus sp. Fp2, Rhodococcus sp. G-74-2, Rhodococcus sp. GK1, Rhodococcus sp. H1, Rhodococcus sp. HR199, Rhodococcus sp. IGT8, Rhodococcus sp. IGTS8, Rhodococcus sp. IOC-77, Rhodococcus sp. J1, Rhodococcus sp. M4, Rhodococcus sp. M-750, Rhodococcus sp. M-777, Rhodococcus sp. N186/21, Rhodococcus sp. N595, Rhodococcus sp. N771, Rhodococcus sp. N-774, Rhodococcus sp. NCIMB 11215, Rhodococcus sp. NCIMB 11216, Rhodococcus sp. NCIMB 12038, Rhodococcus sp. NCIMB12038, Rhodococcus sp. NCIMB 9784, Rhodococcus sp. NDB 1165, Rhodococcus sp. Novo SP361, Rhodococcus sp. P1, Rhodococcus sp. R04, Rhodococcus sp. R312, Rhodococcus sp. RHA1, Rhodococcus sp. S13-4, Rhodococcus sp. SHZ-1, Rhodococcus sp. TK6-recombinant enzyme, pH 8.0652394 2D-image
0.056-acetylxylanRhodotorula mucilaginosa, Rhodotorula mucilaginosa NRC 211003, Rhodotorula mucilaginosa PRL251--23601 2D-image
2.7-Alpha-naphthyl acetateFibrobacter succinogenes, Fibrobacter succinogenes S85, Fibrobacter succinogenes S851--23600 2D-image
7.8-Alpha-naphthyl acetateStreptomyces sp.-pH 6.5701417 2D-image
9.1-Cellulose acetateAspergillus niger-pH 5.0, 45°C650815-
0.36-ethyl acetateNocardia sp., Nocardia sp. 108, Nocardia sp. 239, Nocardia sp. 3CS, Nocardia sp. LSU 169, Nocardia sp. M117, Nocardia sp. NRRL 5646, Nocardia sp. P18.3--35233 2D-image
90-glyceryl triacetateAspergillus niger--35230 2D-image
0.15-methylumbelliferone acetateTrypanosoma brucei--35231 2D-image
0.0067-p-nitrophenyl acetateNocardia sp., Nocardia sp. 108, Nocardia sp. 239, Nocardia sp. 3CS, Nocardia sp. LSU 169, Nocardia sp. M117, Nocardia sp. NRRL 5646, Nocardia sp. P18.3--35233 2D-image
0.023-p-nitrophenyl acetateAspergillus niger--35244 2D-image
0.43-p-nitrophenyl acetateStreptomyces sp.-pH 6.5701417 2D-image
0.9-p-nitrophenyl acetateOrpinomyces sp., Orpinomyces sp. KF7--35242 2D-image
32.5-p-nitrophenyl acetateAcinetobacter sp.-pH 7.0, 25°C, presence of benzethonium chloride666086 2D-image
385-p-nitrophenyl acetateAcinetobacter sp.-pH 7.0, 25°C, absence of benzethonium chloride666086 2D-image
0.07-phenyl acetateRhodococcus sp., Rhodococcus sp. 19070, Rhodococcus sp. 400, Rhodococcus sp. 7, Rhodococcus sp. A81, Rhodococcus sp. AA21, Rhodococcus sp. AIU Z-35-1, Rhodococcus sp. AJ270, Rhodococcus sp. AK32, Rhodococcus sp. AN-22, Rhodococcus sp. ATCC39484, Rhodococcus sp. B1, Rhodococcus sp. C1, Rhodococcus sp. C9, Rhodococcus sp. C-x, Rhodococcus sp. DK17, Rhodococcus sp. DX-35, Rhodococcus sp. ECU0066, Rhodococcus sp. F2-7-3, Rhodococcus sp. Fp2, Rhodococcus sp. G-74-2, Rhodococcus sp. GK1, Rhodococcus sp. H1, Rhodococcus sp. HR199, Rhodococcus sp. IGT8, Rhodococcus sp. IGTS8, Rhodococcus sp. IOC-77, Rhodococcus sp. J1, Rhodococcus sp. M4, Rhodococcus sp. M-750, Rhodococcus sp. M-777, Rhodococcus sp. N186/21, Rhodococcus sp. N595, Rhodococcus sp. N771, Rhodococcus sp. N-774, Rhodococcus sp. NCIMB 11215, Rhodococcus sp. NCIMB 11216, Rhodococcus sp. NCIMB 12038, Rhodococcus sp. NCIMB12038, Rhodococcus sp. NCIMB 9784, Rhodococcus sp. NDB 1165, Rhodococcus sp. Novo SP361, Rhodococcus sp. P1, Rhodococcus sp. R04, Rhodococcus sp. R312, Rhodococcus sp. RHA1, Rhodococcus sp. S13-4, Rhodococcus sp. SHZ-1, Rhodococcus sp. TK6-recombinant enzyme, pH 6.4652394 2D-image
14-TriacetinLactobacillus plantarum--35236 2D-image
0.015-tributyrinEscherichia coliP23872mutant enzyme R48S, in 20 mM phosphate buffer (pH 7.1), at 30°C715950 2D-image
0.018-tributyrinEscherichia coliP23872mutant enzyme R48A, in 20 mM phosphate buffer (pH 7.1), at 30°C715950 2D-image
0.047-tributyrinEscherichia coliP23872mutant enzyme R48K, in 20 mM phosphate buffer (pH 7.1), at 30°C715950 2D-image
0.052-tributyrinEscherichia coliP23872wild type enzyme, in 20 mM phosphate buffer (pH 7.1), at 30°C715950 2D-image
0.073-tributyrinEscherichia coliP23872mutant enzyme R48E, in 20 mM phosphate buffer (pH 7.1), at 30°C715950 2D-image

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
20-2-benzylcyclohex-1-en-1-yl acetateMarchantia polymorpha-isoenzyme Est I, pH 7.0, 35°C666089 2D-image
33-2-benzylcyclohex-1-en-1-yl acetateMarchantia polymorpha-isoenzyme Est II, pH 7.0, 35°C666089 2D-image
63-2-ethylcyclohex-1-en-1-yl acetateMarchantia polymorpha-isoenzyme Est I, pH 7.0, 35°C666089 2D-image
14-2-isopropylcyclohex-1-en-1-yl acetateMarchantia polymorpha-isoenzyme Est I, pH 7.0, 35°C666089 2D-image
77-2-methylcyclohex-1-en-1-yl acetateMarchantia polymorpha-isoenzyme Est I, pH 7.0, 35°C666089 2D-image
26-2-n-pentylcyclohex-1-en-1-yl acetateMarchantia polymorpha-isoenzyme Est II, pH 7.0, 35°C666089 2D-image
42-2-n-pentylcyclohex-1-en-1-yl acetateMarchantia polymorpha-isoenzyme Est I, pH 7.0, 35°C666089 2D-image
79-2-n-propylcyclohex-1-en-1-yl acetateMarchantia polymorpha-isoenzyme Est I, pH 7.0, 35°C666089 2D-image
41.6-2-O-acetyl 4-nitrophenyl beta-D-xylopyranosideCellvibrio japonicus-isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C715029 2D-image
7-2-t-butylcyclohex-1-en-1-yl acetateMarchantia polymorpha-isoenzyme Est I, pH 7.0, 35°C666089 2D-image
17-2-t-butylcyclohex-1-en-1-yl acetateMarchantia polymorpha-isoenzyme Est II, pH 7.0, 35°C666089 2D-image
2101-3-O-acetyl 4-nitrophenyl beta-D-xylopyranosideCellvibrio japonicus-isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C715029-
29-4-nitrophenyl butyrateEscherichia coliP23872mutant enzyme R48K, in 20 mM phosphate buffer (pH 7.1), at 30°C; wild type enzyme, in 20 mM phosphate buffer (pH 7.1), at 30°C715950 2D-image
78-4-nitrophenyl butyrateEscherichia coliP23872mutant enzyme R48E, in 20 mM phosphate buffer (pH 7.1), at 30°C715950 2D-image
86-4-nitrophenyl butyrateEscherichia coliP23872mutant enzyme R48S, in 20 mM phosphate buffer (pH 7.1), at 30°C715950 2D-image
113-4-nitrophenyl butyrateEscherichia coliP23872mutant enzyme R48A, in 20 mM phosphate buffer (pH 7.1), at 30°C715950 2D-image
34.5-4-nitrophenyl hexanoateEscherichia coli-pH 7.1, 65°C, recombinant wild-type enzyme682718 2D-image
48.8-4-nitrophenyl hexanoateEscherichia coli-pH 7.1, 65°C, recombinant mutant T74A682718 2D-image
0.15-4-nitrophenyl-2-O-acetyl-alpha-L-arabinofuranosideAspergillus oryzae-pH 5.5, 45°C680967 2D-image
3.23-4-nitrophenyl-2-O-acetyl-beta-D-xylopyranosideAspergillus oryzae-pH 5.5, 45°C680967 2D-image
1.9-4-nitrophenyl-3-O-acetyl-beta-D-xylopyranosideAspergillus oryzae-pH 5.5, 45°C680967 2D-image
0.91-4-nitrophenyl-4-O-acetyl-beta-D-xylopyranosideAspergillus oryzae-pH 5.5, 45°C680967 2D-image
21-4-nitrophenyl-butanoateEscherichia coli-pH 7.1, 25°C, recombinant wild-type enzyme682718 2D-image
145-4-nitrophenyl-butanoateEscherichia coli-pH 7.1, 65°C, recombinant wild-type enzyme682718 2D-image
155-4-nitrophenyl-butanoateEscherichia coli-pH 7.1, 65°C, recombinant mutant T74A682718 2D-image
241.6-4-O-acetyl 4-nitrophenyl beta-D-xylopyranosideCellvibrio japonicus-isozyme CE2C, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C715029-
1331-4-O-acetyl 4-nitrophenyl beta-D-xylopyranosideClostridium thermocellum-isozyme CE2, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C715029-
3210-4-O-acetyl 4-nitrophenyl beta-D-xylopyranosideCellvibrio japonicus-isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C715029-
12.6-6-acetylmorphineRhodococcus sp., Rhodococcus sp. 19070, Rhodococcus sp. 400, Rhodococcus sp. 7, Rhodococcus sp. A81, Rhodococcus sp. AA21, Rhodococcus sp. AIU Z-35-1, Rhodococcus sp. AJ270, Rhodococcus sp. AK32, Rhodococcus sp. AN-22, Rhodococcus sp. ATCC39484, Rhodococcus sp. B1, Rhodococcus sp. C1, Rhodococcus sp. C9, Rhodococcus sp. C-x, Rhodococcus sp. DK17, Rhodococcus sp. DX-35, Rhodococcus sp. ECU0066, Rhodococcus sp. F2-7-3, Rhodococcus sp. Fp2, Rhodococcus sp. G-74-2, Rhodococcus sp. GK1, Rhodococcus sp. H1, Rhodococcus sp. HR199, Rhodococcus sp. IGT8, Rhodococcus sp. IGTS8, Rhodococcus sp. IOC-77, Rhodococcus sp. J1, Rhodococcus sp. M4, Rhodococcus sp. M-750, Rhodococcus sp. M-777, Rhodococcus sp. N186/21, Rhodococcus sp. N595, Rhodococcus sp. N771, Rhodococcus sp. N-774, Rhodococcus sp. NCIMB 11215, Rhodococcus sp. NCIMB 11216, Rhodococcus sp. NCIMB 12038, Rhodococcus sp. NCIMB12038, Rhodococcus sp. NCIMB 9784, Rhodococcus sp. NDB 1165, Rhodococcus sp. Novo SP361, Rhodococcus sp. P1, Rhodococcus sp. R04, Rhodococcus sp. R312, Rhodococcus sp. RHA1, Rhodococcus sp. S13-4, Rhodococcus sp. SHZ-1, Rhodococcus sp. TK6-recombinant enzyme, pH 8.0652394 2D-image
3-phenyl acetateRhodococcus sp., Rhodococcus sp. 19070, Rhodococcus sp. 400, Rhodococcus sp. 7, Rhodococcus sp. A81, Rhodococcus sp. AA21, Rhodococcus sp. AIU Z-35-1, Rhodococcus sp. AJ270, Rhodococcus sp. AK32, Rhodococcus sp. AN-22, Rhodococcus sp. ATCC39484, Rhodococcus sp. B1, Rhodococcus sp. C1, Rhodococcus sp. C9, Rhodococcus sp. C-x, Rhodococcus sp. DK17, Rhodococcus sp. DX-35, Rhodococcus sp. ECU0066, Rhodococcus sp. F2-7-3, Rhodococcus sp. Fp2, Rhodococcus sp. G-74-2, Rhodococcus sp. GK1, Rhodococcus sp. H1, Rhodococcus sp. HR199, Rhodococcus sp. IGT8, Rhodococcus sp. IGTS8, Rhodococcus sp. IOC-77, Rhodococcus sp. J1, Rhodococcus sp. M4, Rhodococcus sp. M-750, Rhodococcus sp. M-777, Rhodococcus sp. N186/21, Rhodococcus sp. N595, Rhodococcus sp. N771, Rhodococcus sp. N-774, Rhodococcus sp. NCIMB 11215, Rhodococcus sp. NCIMB 11216, Rhodococcus sp. NCIMB 12038, Rhodococcus sp. NCIMB12038, Rhodococcus sp. NCIMB 9784, Rhodococcus sp. NDB 1165, Rhodococcus sp. Novo SP361, Rhodococcus sp. P1, Rhodococcus sp. R04, Rhodococcus sp. R312, Rhodococcus sp. RHA1, Rhodococcus sp. S13-4, Rhodococcus sp. SHZ-1, Rhodococcus sp. TK6-recombinant enzyme, pH 6.4652394 2D-image
6.2-tributyrinEscherichia coliP23872wild type enzyme, in 20 mM phosphate buffer (pH 7.1), at 30°C715950 2D-image
6.9-tributyrinEscherichia coliP23872mutant enzyme R48K, in 20 mM phosphate buffer (pH 7.1), at 30°C715950 2D-image
9.3-tributyrinEscherichia coliP23872mutant enzyme R48E, in 20 mM phosphate buffer (pH 7.1), at 30°C715950 2D-image
17.4-tributyrinEscherichia coliP23872mutant enzyme R48A, in 20 mM phosphate buffer (pH 7.1), at 30°C715950 2D-image
17.5-tributyrinEscherichia coliP23872mutant enzyme R48S, in 20 mM phosphate buffer (pH 7.1), at 30°C715950 2D-image
1.77-4-nitrophenyl-5-O-acetyl-alpha-L-arabinofuranosideAspergillus oryzae-pH 5.5, 45°C680967 2D-image
additional information-4-nitrophenyl-beta-D-xylopyranoside monoacetateHypocrea jecorinaA7J2C6initial rate ratio between 2-, 3-, and 4-O-acetyl 4-nitrophenyl-beta-D-xylopyranoside is 1:19:17.7695758-

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
9.42-2-O-acetyl 4-nitrophenyl beta-D-xylopyranosideCellvibrio japonicus, Cellvibrio japonicus NCIMB 10462-isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C715029315931
1356-3-O-acetyl 4-nitrophenyl beta-D-xylopyranosideCellvibrio japonicus, Cellvibrio japonicus NCIMB 10462-isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C7150290
56.08-4-O-acetyl 4-nitrophenyl beta-D-xylopyranosideCellvibrio japonicus, Cellvibrio japonicus NCIMB 10462-isozyme CE2C, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C7150290
541.5-4-O-acetyl 4-nitrophenyl beta-D-xylopyranosideClostridium thermocellum-isozyme CE2, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C7150290
2599-4-O-acetyl 4-nitrophenyl beta-D-xylopyranosideCellvibrio japonicus, Cellvibrio japonicus NCIMB 10462-isozyme CE2B, in 0.1 M sodium phosphate buffer, pH 6.0, at 40°C7150290

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
12-dimethylarsinic acidRhodococcus sp., Rhodococcus sp. 19070, Rhodococcus sp. 400, Rhodococcus sp. 7, Rhodococcus sp. A81, Rhodococcus sp. AA21, Rhodococcus sp. AIU Z-35-1, Rhodococcus sp. AJ270, Rhodococcus sp. AK32, Rhodococcus sp. AN-22, Rhodococcus sp. ATCC39484, Rhodococcus sp. B1, Rhodococcus sp. C1, Rhodococcus sp. C9, Rhodococcus sp. C-x, Rhodococcus sp. DK17, Rhodococcus sp. DX-35, Rhodococcus sp. ECU0066, Rhodococcus sp. F2-7-3, Rhodococcus sp. Fp2, Rhodococcus sp. G-74-2, Rhodococcus sp. GK1, Rhodococcus sp. H1, Rhodococcus sp. HR199, Rhodococcus sp. IGT8, Rhodococcus sp. IGTS8, Rhodococcus sp. IOC-77, Rhodococcus sp. J1, Rhodococcus sp. M4, Rhodococcus sp. M-750, Rhodococcus sp. M-777, Rhodococcus sp. N186/21, Rhodococcus sp. N595, Rhodococcus sp. N771, Rhodococcus sp. N-774, Rhodococcus sp. NCIMB 11215, Rhodococcus sp. NCIMB 11216, Rhodococcus sp. NCIMB 12038, Rhodococcus sp. NCIMB12038, Rhodococcus sp. NCIMB 9784, Rhodococcus sp. NDB 1165, Rhodococcus sp. Novo SP361, Rhodococcus sp. P1, Rhodococcus sp. R04, Rhodococcus sp. R312, Rhodococcus sp. RHA1, Rhodococcus sp. S13-4, Rhodococcus sp. SHZ-1, Rhodococcus sp. TK6-recombinant enzyme, pH 6.4652394 2D-image

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
0.000109-Hypocrea jecorinaA7J2C64-nitrophenyl butyrate, purified recombinant enzyme695758
0.000213-Hypocrea jecorinaA7J2C64-nitrophenyl formate, purified recombinant enzyme695758
0.000225-Wickerhamomyces anomalus AL112, Wickerhamomyces anomalus K, Wickerhamomyces anomalus, Wickerhamomyces anomalus NCYC 434, Wickerhamomyces anomalus SL-125-mutant strain, ethyl acetate-hydrolysing esterase (EAHase) activity698520
0.000236-Wickerhamomyces anomalus AL112, Wickerhamomyces anomalus K, Wickerhamomyces anomalus, Wickerhamomyces anomalus NCYC 434, Wickerhamomyces anomalus SL-125-mutant strain, isoamyl acetate-hydrolysing esterase (IAHase) activity698520
0.000342-Wickerhamomyces anomalus-wild-type strain, ethyl acetate-hydrolysing esterase (EAHase) activity698520
0.000347-Wickerhamomyces anomalus AL112, Wickerhamomyces anomalus K, Wickerhamomyces anomalus NCYC 434, Wickerhamomyces anomalus SL-125, Wickerhamomyces anomalus-wild-type strain, isoamyl acetate-hydrolysing esterase (IAHase) activity698520
0.021-Candida bororiensis--35232
0.027-Eleusine coracana-purified native enzyme, substrate ragi680319
0.061-Eleusine coracana-purified native enzyme, substrate larch wood xylan680319
0.082-Acinetobacter sp. 15150, Acinetobacter sp. 210A, Acinetobacter sp. 3B-1, Acinetobacter sp. 4B, Acinetobacter sp. AG, Acinetobacter sp. AK226, Acinetobacter sp. C-17, Acinetobacter sp. C1W, Acinetobacter sp. CGMCC, Acinetobacter sp. CHC, Acinetobacter sp. CHE4-1, Acinetobacter sp. CR9, Acinetobacter sp. CRH-104D, Acinetobacter sp. DV1, Acinetobacter sp. HO1-N, Acinetobacter sp. JCI, Acinetobacter sp. M-1, Acinetobacter sp. M-30, Acinetobacter sp. MTCC 6816, Acinetobacter sp. NCIB, Acinetobacter sp. NCIMB 9871, Acinetobacter sp. SA1, Acinetobacter sp. ST-1, Acinetobacter sp., Acinetobacter sp. TD63, Acinetobacter sp. YAA-substrate p-nitrophenyl laurate, pH 7.0, 25°C666086
0.21-Hypocrea jecorinaA7J2C64-nitrophenyl acetate, over-expression culture supernatant; recombinant enzyme, culture supernatant695758
0.251-Acinetobacter sp.-substrate p-nitrophenyl acetate, pH 7.0, 25°C666086
0.282-Eleusine coracana-purified native enzyme, substrate gum karaya680319
0.297-Acinetobacter sp. 15150, Acinetobacter sp. 210A, Acinetobacter sp. 3B-1, Acinetobacter sp. 4B, Acinetobacter sp. AG, Acinetobacter sp. AK226, Acinetobacter sp. C-17, Acinetobacter sp. C1W, Acinetobacter sp. CGMCC, Acinetobacter sp. CHC, Acinetobacter sp. CHE4-1, Acinetobacter sp. CR9, Acinetobacter sp. CRH-104D, Acinetobacter sp. DV1, Acinetobacter sp. HO1-N, Acinetobacter sp. JCI, Acinetobacter sp. M-1, Acinetobacter sp. M-30, Acinetobacter sp. MTCC 6816, Acinetobacter sp. NCIB, Acinetobacter sp. NCIMB 9871, Acinetobacter sp. SA1, Acinetobacter sp. ST-1, Acinetobacter sp., Acinetobacter sp. TD63, Acinetobacter sp. YAA-substrate p-nitrophenyl decanoate, pH 7.0, 25°C666086
0.352-Acinetobacter sp.-substrate p-nitrophenyl hexanoate, pH 7.0, 25°C666086
0.369-Acinetobacter sp. 15150, Acinetobacter sp. 210A, Acinetobacter sp. 3B-1, Acinetobacter sp. 4B, Acinetobacter sp. AG, Acinetobacter sp. AK226, Acinetobacter sp. C-17, Acinetobacter sp. C1W, Acinetobacter sp. CGMCC, Acinetobacter sp. CHC, Acinetobacter sp. CHE4-1, Acinetobacter sp. CR9, Acinetobacter sp. CRH-104D, Acinetobacter sp. DV1, Acinetobacter sp. HO1-N, Acinetobacter sp. JCI, Acinetobacter sp. M-1, Acinetobacter sp. M-30, Acinetobacter sp. MTCC 6816, Acinetobacter sp. NCIB, Acinetobacter sp. NCIMB 9871, Acinetobacter sp. SA1, Acinetobacter sp. ST-1, Acinetobacter sp., Acinetobacter sp. TD63, Acinetobacter sp. YAA-substrate p-nitrophenyl propionate, pH 7.0, 25°C666086
0.474-Acinetobacter sp.-substrate p-nitrophenyl butyrate, pH 7.0, 25°C666086
0.8-Drosophila mojavensis--35228
0.85-Hypocrea jecorinaA7J2C6recombinant enzyme, after CM Sepharose FF column695758
1.12-Hypocrea jecorinaA7J2C6recombinant enzyme, after DEAE FF column, 4-nitrophenyl acetate695758
1.21-Hypocrea jecorinaA7J2C64-nitrophenyl acetate, purified recombinant enzyme695758
2-Cellvibrio japonicus, Cellvibrio japonicus NCIMB 10462-isozyme CE2C, using methyl 2,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C715029
2-Clostridium thermocellum-isozyme CE2, using methyl 2,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C715029
3-Cellvibrio japonicus, Cellvibrio japonicus NCIMB 10462-isozyme CE2C, using 6-O-acetyl-D-mannopyranose as substrate, pH 6.0, 40°C; isozyme CE2C, using methyl 3,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C715029
3.38-Eleusine coracana-purified native enzyme, substrate alpha-naphthyl acetate680319
4-Cellvibrio japonicus, Cellvibrio japonicus NCIMB 10462-isozyme CE2B, using methyl 3,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C715029
4.44-Eleusine coracana-purified native enzyme, substrate wheat680319
5-Aspergillus niger--35230
5-Cellvibrio japonicus, Cellvibrio japonicus NCIMB 10462-isozyme CE2B, using methyl 2,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C715029
5.39-Eleusine coracana-purified native enzyme, substrate 4-nitrophenyl acetate680319
7.87-Lactobacillus plantarum--35236
8-Clostridium thermocellum-isozyme CE2, using methyl 3,4-O-diacetyl-beta-D-xylopyranoside as substrate, pH 6.0, 40°C715029
9.63-Streptomyces sp.-p-nitrophenyl acetate, after hydroxyapatite chromatography, pH 6.5701417
20.5-Hypocrea jecorinaA7J2C64-nitrophenyl acetate, maximum velocity, purified recombinant enzyme, 30°C, pH 5.8695758
31-Aspergillus niger--23596
35-Clostridium thermocellum-isozyme CE2, using 6-O-acetyl-D-mannopyranose as substrate, pH 6.0, 40°C715029
46.5-Bacillus subtilis-mutant E188W/M193C, using 4-nitrophenyl acetate as substrate695582
60-Cellvibrio japonicus-isozyme CE2C, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40°C715029
71-Streptomyces sp.-p-nitrophenyl acetate, pH 6.5701417
71.4-Meyerozyma guilliermondii-purified enzyme653087
110.9-Streptomyces olivochromogenes, Streptomyces olivochromogenes IFO3178, Streptomyces olivochromogenes NRCC 2258--35193
118.1-Penicillium purpurogenum-AXE I23594
118.8-Bacillus pumilus, Bacillus pumilus 76, Bacillus pumilus A8, Bacillus pumilus B26, Bacillus pumilus BK2, Bacillus pumilus Bn-262, Bacillus pumilus CECT 5072, Bacillus pumilus DKS1, Bacillus pumilus HL721, Bacillus pumilus IPO, Bacillus pumilus P9, Bacillus pumilus PS213, Bacillus pumilus PS214--23597
139.8-Penicillium purpurogenum-AXE II23594
183-Cellvibrio japonicus, Clostridium thermocellum-isozyme CE2, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40°C715029
250-Escherichia coli-pH 7.1, 65°C, purified recombinant wild-type enzyme682718
270-Escherichia coli-pH 7.1, 65°C, purified recombinant mutant T74A682718
324-Kluyveromyces marxianus-(R,S)-1,2-O-isopropylidene glycol acetate, EST1, after preparative electrophoresis699069
328-Cellvibrio japonicus-isozyme CE2B, using methyl 6-O-acetyl-beta-D-glucopyranoside as substrate, pH 6.0, 40°C715029
333-Bacillus subtilis-wild-type, using 4-nitrophenyl acetate as substrate695582
376-Bos taurus--35224
395-Cellvibrio japonicus-isozyme CE2B, using 6-O-acetyl-D-mannopyranose as substrate, pH 6.0, 40°C715029
715-Streptomyces lividans--35239
1027-Streptomyces sp.-alpha-naphthyl acetate, pH 6.5701417
1104-Nocardia sp., Nocardia sp. 108, Nocardia sp. 239, Nocardia sp. 3CS, Nocardia sp. LSU 169, Nocardia sp. M117, Nocardia sp. NRRL 5646, Nocardia sp. P18.3--35233
2933-Fibrobacter succinogenes, Fibrobacter succinogenes S85, Fibrobacter succinogenes S851--23600
16050-Aspergillus niger-purified enzyme650815
additional information-Bacillus subtilis--35235
additional information-Aspergillus niger-substrate specificity650815
additional information-Cichorium intybus-isoforms with pI-values 4.2 and 5.0 are the most active ones in all samples665872
additional information-Aspergillus oryzae--680967
additional information-Hypocrea jecorinaA7J2C6extremly low activities against 4-nitrophenyl formate, butyrate, or canoate695758
additional information-Wickerhamomyces anomalus AL112, Wickerhamomyces anomalus K, Wickerhamomyces anomalus, Wickerhamomyces anomalus NCYC 434, Wickerhamomyces anomalus SL-125-mutant strain, 61% lower ethyl acetate-hydrolysing esterase (EAHase) activity than in wild-type strain; mutant strain, 68% lower isoamyl acetate-hydrolysing esterase (IAHase) activity than in wild-type strain698520
additional information-Kluyveromyces marxianus B57, Kluyveromyces marxianus CBS 1553, Kluyveromyces marxianus CBS6556, Kluyveromyces marxianus CCEBI 2011, Kluyveromyces marxianus DSM5418, Kluyveromyces marxianus IMB3, Kluyveromyces marxianus NBIMCC 1984, Kluyveromyces marxianus NRRL Y-7571-(R)-1,2-O-isopropylidene glycol acetate, EST1, 1.0 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1; (R,S)-1,2-O-isopropylidene glycol acetate as substrate for EST1, 20 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1; (R,S)-1,2-O-isopropylidene glycol butyrate as substrate for EST1, 4.6 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1; (R,S)-1,2-O-isopropylidene glycol caproate as substrate for EST1, 0.7 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1699069
additional information-Kluyveromyces marxianus-(R)-1,2-O-isopropylidene glycol acetate, EST1, 1.0 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1; (R,S)-1,2-O-isopropylidene glycol acetate as substrate for EST1, 20 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1; (R,S)-1,2-O-isopropylidene glycol butyrate as substrate for EST1, 4.6 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1; (R,S)-1,2-O-isopropylidene glycol caproate as substrate for EST1, 0.7 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1; (S)-1,2-O-isopropylidene glycol acetate, EST1, 1.2 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1; triacetin as substrate for EST1, 4.06 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1; tributyrin as substrate for EST1, 2.8 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1699069
additional information-Kluyveromyces marxianus UNSW 510700, Kluyveromyces marxianus Y-488-(R)-1,2-O-isopropylidene glycol acetate, EST1, 1.0 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1; (R,S)-1,2-O-isopropylidene glycol acetate as substrate for EST1, 20 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1; (R,S)-1,2-O-isopropylidene glycol butyrate as substrate for EST1, 4.6 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1; (R,S)-1,2-O-isopropylidene glycol caproate as substrate for EST1, 0.7 micromol/min by activity corresponding to synthesis of 0.01 micromol p-nitrophenol/min/mg EST1699069

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
4.56Aspergillus oryzae--680967
5.3-Penicillium purpurogenum-AXE I23594
5.56Aspergillus niger--23596
5.5-Hypocrea jecorina--35223
5.5-Aspergillus niger--650815
6-Penicillium purpurogenum-AXE II23594
6.2-Vitis vinifera-assay at678012
6.7-Lactobacillus plantarum--35236
7-Fibrobacter succinogenes--23600
7-Aspergillus niger--35230, 35244
7.1-Escherichia coli--682718
7.59Nocardia sp.--35233
7.5-Streptomyces lividans--35239
7.5-Meyerozyma guilliermondii--653087
7.5-Eleusine coracana--680319
7.5-Escherichia coli-assay at682897
810Rhodotorula mucilaginosa--23601
8-Bacillus pumilus--23597
8-Acinetobacter sp.--666086
8.5-Cymbopogon martinii-assay at, substrate geranyl acetate653450
9-Orpinomyces sp.--35242
9-Kluyveromyces marxianus-EST1 sample after anion-exchange chromatography, p-nitrophenyl acetate as substrate, 20°C699069

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
3-Aspergillus niger-no activity below35230
55.5Aspergillus oryzae--35241
5-Kluyveromyces marxianus-inactive below, EST1 sample after anion-exchange chromatography, p-nitrophenyl acetate as substrate, 20°C, 4 h699069
6-Streptomyces sp.-85% residual activity, alpha-naphthyl acetate as substrate, more active in acetate buffer than phosphate buffer701417
6.57Streptomyces sp.-optimum activity, alpha-naphthyl acetate as substrate701417
710Kluyveromyces marxianus-100% activity, EST1 sample after anion-exchange chromatography, p-nitrophenyl acetate as substrate, 20°C, 4 h699069
7-Hypocrea jecorina-no activity above35223
8-Streptomyces sp.-65% residual activity, alpha-naphthyl acetate as substrate701417
11-Kluyveromyces marxianus-16% activity, EST1 sample after anion-exchange chromatography, p-nitrophenyl acetate as substrate, 20°C, 4 h699069

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
22-Vitis vinifera, Vitis vinifera Cabernet Sauvignon, Vitis vinifera cv. Gewuerztraminer, Vitis vinifera PN40024-assay at room temperature678012
30-Orpinomyces sp., Orpinomyces sp. KF7--35242
30-Cymbopogon martinii-assay at, substrate geranyl acetate653450
35-Aspergillus niger--35244
37-Alphacoronavirus, Alphacoronavirus SARS-CoV-respiratory virus enzyme652759
39-Alphacoronavirus, Alphacoronavirus SARS-CoV-enteropathogenic virus enzyme652759
40-Aspergillus niger--35230
40-Lactobacillus plantarum, Lactobacillus plantarum 124-2, Lactobacillus plantarum AKU1009a, Lactobacillus plantarum ATCC 14917, Lactobacillus plantarum B38, Lactobacillus plantarum CECT 748T, Lactobacillus plantarum CECT 748(T), Lactobacillus plantarum DBPZ0987, Lactobacillus plantarum IAM 12477, Lactobacillus plantarum L137, Lactobacillus plantarum LA 318, Lactobacillus plantarum LM3, Lactobacillus plantarum Lp60, Lactobacillus plantarum Lp80, Lactobacillus plantarum NCIMB 8826, Lactobacillus plantarum NCIMB 8826 delta ldhL1 -xpk1::tkt-delta xpk, Lactobacillus plantarum NRIC 1067T, Lactobacillus plantarum ST-III, Lactobacillus plantarum WFCS1--35236
45-Fibrobacter succinogenes, Fibrobacter succinogenes S85, Fibrobacter succinogenes S851--23600
45-Eleusine coracana--680319
5060Meyerozyma guilliermondii, Meyerozyma guilliermondii B10-05, Meyerozyma guilliermondii FTI, Meyerozyma guilliermondii FTI 20037, Meyerozyma guilliermondii H17, Meyerozyma guilliermondii NRRL Y-17257, Meyerozyma guilliermondii OUC1, Meyerozyma guilliermondii PYCC 3012, Meyerozyma guilliermondii UFMG-Y65, Meyerozyma guilliermondii Y-1017--653087
50-Penicillium purpurogenum, Penicillium purpurogenum KJS506-AXE I23594
50-Aspergillus niger--23596
50-Hypocrea jecorina, Hypocrea jecorina ALKO2877, Hypocrea jecorina CBH I, Hypocrea jecorina Cel6A, Hypocrea jecorina CL847, Hypocrea jecorina FTKO-39, Hypocrea jecorina PC-3-7, Hypocrea jecorina QM6a, Hypocrea jecorina QM 9414, Hypocrea jecorina QM9414, Hypocrea jecorina RUT C-30, Hypocrea jecorina RUT C30, Hypocrea jecorina RUT-C30, Hypocrea jecorina ZU-02--35223
50-Aspergillus niger--650815
50-Aspergillus oryzae--680967
50-Streptomyces sp.--701417
55-Bacillus pumilus, Bacillus pumilus 76, Bacillus pumilus A8, Bacillus pumilus B26, Bacillus pumilus BK2, Bacillus pumilus Bn-262, Bacillus pumilus CECT 5072, Bacillus pumilus DKS1, Bacillus pumilus HL721, Bacillus pumilus IPO, Bacillus pumilus P9, Bacillus pumilus PS213, Bacillus pumilus PS214--23597
60-Penicillium purpurogenum-AXE II23594
60-Acinetobacter sp., Acinetobacter sp. 15150, Acinetobacter sp. 210A, Acinetobacter sp. 3B-1, Acinetobacter sp. 4B, Acinetobacter sp. AG, Acinetobacter sp. AK226, Acinetobacter sp. C-17, Acinetobacter sp. C1W, Acinetobacter sp. CGMCC, Acinetobacter sp. CHC, Acinetobacter sp. CHE4-1, Acinetobacter sp. CR9, Acinetobacter sp. CRH-104D, Acinetobacter sp. DV1, Acinetobacter sp. HO1-N, Acinetobacter sp. JCI, Acinetobacter sp. M-1, Acinetobacter sp. M-30, Acinetobacter sp. MTCC 6816, Acinetobacter sp. NCIB, Acinetobacter sp. NCIMB 9871, Acinetobacter sp. SA1, Acinetobacter sp. ST-1, Acinetobacter sp. TD63, Acinetobacter sp. YAA--666086
65-Escherichia coli--682718
70-Streptomyces lividans, Streptomyces lividans 66, Streptomyces lividans 66strain 1326, Streptomyces lividans 8, Streptomyces lividans MTCC1, Streptomyces lividans TK64--35239
80-Kluyveromyces marxianus, Kluyveromyces marxianus B57, Kluyveromyces marxianus CBS 1553, Kluyveromyces marxianus CBS6556, Kluyveromyces marxianus CCEBI 2011, Kluyveromyces marxianus DSM5418, Kluyveromyces marxianus IMB3, Kluyveromyces marxianus NBIMCC 1984, Kluyveromyces marxianus NRRL Y-7571, Kluyveromyces marxianus UNSW 510700, Kluyveromyces marxianus Y-488-100% relative activity, at 60-70°C 90% relative activity, EST1 sample after anion-exchange chromatography, p-nitrophenyl acetate as substrate, pH 7, 3 min699069

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
4-Rhodotorula mucilaginosa, Rhodotorula mucilaginosa NRC 211003, Rhodotorula mucilaginosa PRL251-no activity below23601
1575Escherichia coli--682718
4065Streptomyces sp., Streptomyces sp. 142, Streptomyces sp. 17-1, Streptomyces sp. 174, Streptomyces sp. 2B, Streptomyces sp. 3, Streptomyces sp. 3022a, Streptomyces sp. 594, Streptomyces sp. 65, Streptomyces sp. A19249, Streptomyces sp. A5, Streptomyces sp. AA586, Streptomyces sp. AB3534, Streptomyces sp. AKU 2801, Streptomyces sp. ALG-5, Streptomyces sp. C-248, Streptomyces sp. C-254, Streptomyces sp. Chainia, Streptomyces sp. CL190, Streptomyces sp. CS326, Streptomyces sp. CS-57, Streptomyces sp. E750-3, Streptomyces sp. E-86, Streptomyces sp. FLA, Streptomyces sp. FR-008, Streptomyces sp. G26, Streptomyces sp. GERI-155, Streptomyces sp. IN1, Streptomyces sp. J-13-3, Streptomyces sp. J-350P, Streptomyces sp. J-845S, Streptomyces sp. K11, Streptomyces sp. K37, Streptomyces sp. KB210-8SY, Streptomyces sp. ki-8, Streptomyces sp. KJ-72, Streptomyces sp. KO-3988, Streptomyces sp. KT-23, Streptomyces sp. L-13, Streptomyces sp. L-1689-23, Streptomyces sp. LL-B7, Streptomyces sp. M-03, Streptomyces sp. M-20, Streptomyces sp. M23, Streptomyces sp. MCI-2524, Streptomyces sp. MTCC 7546, Streptomyces sp. NCIM 2730, Streptomyces sp. NCIM 5127, Streptomyces sp. NL15-2K, Streptomyces sp. No. 3137, Streptomyces sp. No. 6, Streptomyces sp. NRRL 5331, Streptomyces sp. OH-11242, Streptomyces sp. OH11242, Streptomyces sp. PCB7, Streptomyces sp. R-39, Streptomyces sp. R39, Streptomyces sp. RC 1071, Streptomyces sp. S38, Streptomyces sp. SA-COO, Streptomyces sp. SCC-2136, Streptomyces sp. strainTH1, Streptomyces sp. T109-3, Streptomyces sp. TH1, Streptomyces sp. TH-4, Streptomyces sp. UC5319, Streptomyces sp. WM-23, Streptomyces sp. X9, Streptomyces sp. Z-11-6--701417

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
3-Aspergillus niger-below, isoelectric focusing650815
3.6-Aspergillus oryzae-isoelectric focusing680967
5.9-Hypocrea jecorinaA7J2C6theoretical695758
6-Hypocrea jecorinaA7J2C6purified recombinant enzyme695758
7.6-Meyerozyma guilliermondii-isoelectric focusing653087
additional information-Cichorium intybus-all isoforms isolated have pI-values between 4.1 and 5.2665872

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
adultHomo sapiens-ubiquitous transcript of ca. 2.4 kb, highest gene expression level in liver, placenta and heart, Northern blot696095Manually annotated by BRENDA team
artery wallHomo sapiens-kidney, cytochemistry and confocal microscopy696095Manually annotated by BRENDA team
commercial preparationAspergillus niger--650815Manually annotated by BRENDA team
culture supernatantHypocrea jecorinaA7J2C6-695758Manually annotated by BRENDA team
embryoHomo sapiens-ubiquitous transcript of ca. 2.4 kb, highest gene expression level in liver and kidney, Northern blot696095Manually annotated by BRENDA team
endothelial cellHomo sapiens-kidney, cytochemistry and confocal microscopy696095Manually annotated by BRENDA team
HEK-293 cellHomo sapiens-lower expression level than in Hep-G2 cells as revealed by Northern blot and quantitative RT-PCR, serum-induced inhibition of gene expression (quantitative RT-PCR, FACS, reporter gene assay)696095Manually annotated by BRENDA team
Hep-G2 cellHomo sapiens-strong expression, lower expression level than in liver as revealed by Northern blot and quantitative RT-PCR, cell fractionation, FACS analysis696095Manually annotated by BRENDA team
huskCitrus sinensis--663817Manually annotated by BRENDA team
inflorescenceCymbopogon martinii-spikelets, maximal in immature florescences, isozyme Est-B is increased during development, activity of isozymes at different developmental stages, overview653450Manually annotated by BRENDA team
kidneyHomo sapiens-lower expression level than in liver as revealed by Northern blot and quantitative RT-PCR, Western blot, strong in glomeruli and tubular structures according to cytochemistry and confocal microscopy696095Manually annotated by BRENDA team
leafVitis vinifera, Vitis vinifera Cabernet Sauvignon, Vitis vinifera cv. Gewuerztraminer, Vitis vinifera PN40024-young678012Manually annotated by BRENDA team
leaf budVitis vinifera, Vitis vinifera Cabernet Sauvignon, Vitis vinifera cv. Gewuerztraminer, Vitis vinifera PN40024--678012Manually annotated by BRENDA team
leukocyteHomo sapiens-no expression in granulocytes and lymphocytes according to cell surface domain-specific FACS analyses696095Manually annotated by BRENDA team
myceliumHypocrea jecorinaA7J2C6high transcript level induced by 4 mM sophorose, 1% (w/v) cellulose, 1% (w/v) oat spelt xylan, or 1% (w/v) lactose, significant transcript levels induced by 1% (w/v) L-arabinose, low transcript levels induced by acetic acid (4 mM sodium acetate, 1% (w/v) glycerol), revealed by Northern blot695758Manually annotated by BRENDA team
pancreatic isletHomo sapiens-highest expression within pancreatic tissue at the islets of Langerhans, revealed by cytochemistry and confocal microscopy696095Manually annotated by BRENDA team
peripheral blood mononuclear cellHomo sapiens-high expression on monocytes according to cell-surface domain-specific FACS analyses696095Manually annotated by BRENDA team
placentaHomo sapiens-high expression level, Northern blot, quantitative RT-PCR696095Manually annotated by BRENDA team
RINm5F cellHomo sapiens-cell fractionation696095Manually annotated by BRENDA team
rootCichorium intybus--665872Manually annotated by BRENDA team
SH-SY5Y cellHomo sapiens-quantitative RT-PCR, Western blot696095Manually annotated by BRENDA team
urineHomo sapiens-ELISA using cell surface domain-specific antibody696095Manually annotated by BRENDA team
vascular smooth muscle cellHomo sapiens-kidney, cytochemistry and confocal microscopy696095Manually annotated by BRENDA team
liverHomo sapiens-highest expression level as revealed by Northern blot and quantitative RT-PCR, Western blot, cytochemistry and confocal microscopy696095Manually annotated by BRENDA team
additional informationEleusine coracana-acetic acid esterase from 72 h ragi malt680319Manually annotated by BRENDA team

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
cell membraneHomo sapiens-heavy membrane after sucrose density gradient cell fractionation on Hep-G2 (full-length protein and 32 kDa variant) and RINm5F cells (full-length protein)-696095Manually annotated by BRENDA team
cell wallCichorium intybus--5618665872Manually annotated by BRENDA team
cytoplasmTrypanosoma brucei--573735231Manually annotated by BRENDA team
cytoplasmBacillus subtilis--573735235Manually annotated by BRENDA team
cytosolHomo sapiens-32 kDa low molecular weight variant, sucrose density gradient cell fractionation on RINm5F cells5829696095Manually annotated by BRENDA team
extracellularRhodotorula mucilaginosa, Rhodotorula mucilaginosa NRC 211003, Rhodotorula mucilaginosa PRL251---23601Manually annotated by BRENDA team
extracellularSchizophyllum commune, Schizophyllum commune AS 5.391, Schizophyllum commune ATCC 38548---23607Manually annotated by BRENDA team
extracellularHypocrea jecorina, Hypocrea jecorina ALKO2877, Hypocrea jecorina CBH I, Hypocrea jecorina Cel6A, Hypocrea jecorina CL847, Hypocrea jecorina FTKO-39, Hypocrea jecorina PC-3-7, Hypocrea jecorina QM6a, Hypocrea jecorina QM 9414, Hypocrea jecorina QM9414, Hypocrea jecorina RUT C-30, Hypocrea jecorina RUT C30, Hypocrea jecorina RUT-C30, Hypocrea jecorina ZU-02---35223Manually annotated by BRENDA team
extracellularBacillus subtilis---35235Manually annotated by BRENDA team
extracellularMeyerozyma guilliermondii, Meyerozyma guilliermondii B10-05, Meyerozyma guilliermondii FTI, Meyerozyma guilliermondii FTI 20037, Meyerozyma guilliermondii H17, Meyerozyma guilliermondii NRRL Y-17257, Meyerozyma guilliermondii OUC1, Meyerozyma guilliermondii PYCC 3012, Meyerozyma guilliermondii UFMG-Y65, Meyerozyma guilliermondii Y-1017---653087Manually annotated by BRENDA team
extracellularHypocrea jecorinaA7J2C6; 19 N-terminal residues serve as signal peptide for secretion-695758Manually annotated by BRENDA team
extracellularHomo sapiens-C-terminus of full-length protein (residues 62-416) as revealed by cell surface domain-specific FACS analyses on monocytes and Hep-G2 cells as well as cell surface domain-specific cytochemistry and confocal microscopy on liver, kidney, and pancreas, detection by ELISA in urine and supernatant of thrombin-treated Hep-G2 cells-696095Manually annotated by BRENDA team
additional informationAlphacoronavirus, Alphacoronavirus SARS-CoV-viruses are located in the cytoplasm and at perinuclear sites in the transfected COS-7 cells-652759Manually annotated by BRENDA team
additional informationHomo sapiens-not detectable by ELISA using cell surface domain-specific antibody-696095Manually annotated by BRENDA team

PDBSCOPCATHORGANISM
3fvr, downloadSCOP (3fvr)CATH (3fvr)Bacillus pumilus
3fvt, downloadSCOP (3fvt)CATH (3fvt)Bacillus pumilus
3fyt, downloadSCOP (3fyt)CATH (3fyt)Bacillus pumilus
3fyu, downloadSCOP (3fyu)CATH (3fyu)Bacillus pumilus
1bs9, downloadSCOP (1bs9)CATH (1bs9)Penicillium purpurogenum
1g66, downloadSCOP (1g66)CATH (1g66)Penicillium purpurogenum
2axe, downloadSCOP (2axe)CATH (2axe)Penicillium purpurogenum

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
29000-Kluyveromyces marxianus B57, Kluyveromyces marxianus CBS 1553, Kluyveromyces marxianus CBS6556, Kluyveromyces marxianus CCEBI 2011, Kluyveromyces marxianus DSM5418, Kluyveromyces marxianus IMB3, Kluyveromyces marxianus NBIMCC 1984, Kluyveromyces marxianus NRRL Y-7571, Kluyveromyces marxianus, Kluyveromyces marxianus UNSW 510700, Kluyveromyces marxianus Y-488-preparative electrophoresis-purified EST1, SDS-PAGE699069
30000-Homo sapiens-degradation product, Western blot, not detectable by cytosolic domain-specific antibody696095
32000-Homo sapiens-low molecular variant, membrane associated (Hep-G2 cell) or cytosolic (RINm5F cell), Western blot696095
35000-Petroselinum crispum-gel filtration35199
35000-Candida bororiensis-gel filtration35232
35000-Aspergillus niger-gel filtration35244
37090-Hypocrea jecorinaA7J2C6theoretical, polypeptide of 348 amino acids695758
38000-Streptomyces sp.-SDS-PAGE701417
40000-Aspergillus oryzae-gel filtration35241
42000-Hypocrea jecorinaA7J2C6over-expression in Hypocrea jecorina Rut-C30, SDS-PAGE, molecular weight shift of purified recombinant enzyme (below theoretical weight) upon treatment with endoglycosidase H695758
45000-Marchantia polymorpha-gel filtration, isoenzyme Est II666089
45000-Hypocrea jecorinaA7J2C6SDS-PAGE695758
50000-Homo sapiens-full-length protein, Western blot696095
51000-Bacillus subtilis-gel filtration35235
52000-Homo sapiens-high molecular variant in Hep-G2 and SH-SY5Y cells, Western blot696095
54000-Marchantia polymorpha-gel filtration, isoenzyme Est I666089
65000-Meyerozyma guilliermondii B10-05, Meyerozyma guilliermondii FTI 20037, Meyerozyma guilliermondii FTI, Meyerozyma guilliermondii, Meyerozyma guilliermondii H17, Meyerozyma guilliermondii NRRL Y-17257, Meyerozyma guilliermondii OUC1, Meyerozyma guilliermondii PYCC 3012, Meyerozyma guilliermondii UFMG-Y65, Meyerozyma guilliermondii Y-1017-gel filtration653087
6700068000Hypocrea jecorina ALKO2877, Hypocrea jecorina CBH I, Hypocrea jecorina Cel6A, Hypocrea jecorina CL847, Hypocrea jecorina FTKO-39, Hypocrea jecorina, Hypocrea jecorina PC-3-7, Hypocrea jecorina QM6a, Hypocrea jecorina QM 9414, Hypocrea jecorina QM9414, Hypocrea jecorina RUT C-30, Hypocrea jecorina RUT C30, Hypocrea jecorina RUT-C30, Hypocrea jecorina ZU-02-gel filtration35223
6700068000Bos taurus-gel filtration35224
78500-Sclerotinia sp.-gel filtration35234
79400-Eleusine coracana-gel filtration680319
84000-Nocardia sp. 108, Nocardia sp. 239, Nocardia sp. 3CS, Nocardia sp. LSU 169, Nocardia sp. M117, Nocardia sp. NRRL 5646, Nocardia sp. P18.3, Nocardia sp.-sucrose density gradient centrifugation35233
8500095000Drosophila mojavensis-gel filtration35228
155000-Streptomyces sp.-native, gel-filtration on Sephacryl S-200 HR column701417
160000-Aspergillus niger-gel filtration35230
160000-Bacillus subtilis-gel filtration35235
190000-Bacillus pumilus-gel filtration23597
additional information-Vitis vinifera Cabernet Sauvignon, Vitis vinifera cv. Gewuerztraminer, Vitis vinifera, Vitis vinifera PN40024-esterase isozyme analysis by native PAGE678012

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
?Citrus sinensis-x * 45000, SDS-PAGE663817
?Cichorium intybus-x * 35000, x * 50000, and x * 65000, SDS-PAGE of enzyme isoforms665872
?Acinetobacter sp. 15150, Acinetobacter sp. 210A, Acinetobacter sp. 3B-1, Acinetobacter sp. 4B, Acinetobacter sp. AG, Acinetobacter sp. AK226, Acinetobacter sp. C-17, Acinetobacter sp. C1W, Acinetobacter sp. CGMCC, Acinetobacter sp. CHC, Acinetobacter sp. CHE4-1, Acinetobacter sp. CR9, Acinetobacter sp. CRH-104D, Acinetobacter sp. DV1, Acinetobacter sp. HO1-N, Acinetobacter sp. JCI, Acinetobacter sp. M-1, Acinetobacter sp. M-30, Acinetobacter sp. MTCC 6816, Acinetobacter sp. NCIB, Acinetobacter sp. NCIMB 9871, Acinetobacter sp. SA1, Acinetobacter sp. ST-1, Acinetobacter sp. TD63, Acinetobacter sp., Acinetobacter sp. YAA-x * 20000, SDS-PAGE666086
?Aspergillus oryzae-x * 30000, SDS-PAGE680967
dimerDrosophila mojavensis-2 * 62000-64000, SDS-PAGE35228
dimerNocardia sp. 108, Nocardia sp. 239, Nocardia sp., Nocardia sp. 3CS, Nocardia sp. LSU 169, Nocardia sp. M117, Nocardia sp. NRRL 5646, Nocardia sp. P18.3-2 * 39500, SDS-PAGE35233
dimerMarchantia polymorpha-2 * 27000, isoenzyme EstI, 2 * 22500, isoenzyme Est II, SDS-PAGE666089
dimerEscherichia coli-determined by affinity using pull down701087
monomerPetroselinum crispum-1 * 36000, SDS-PAGE35199
monomerBos taurus-1 * 70000, SDS-PAGE35224
monomerAspergillus oryzae-1 * 36000, SDS-PAGE35241
monomerAspergillus niger-1 * 32000, SDS-PAGE35244
multimerBacillus pumilus-x * 40000, SDS-PAGE23597
tetramerEleusine coracana-4 * 19700, SDS-PAGE680319
tetramerStreptomyces sp.-4 * 38000, gel filtration on Sephacryl S-200 HR column701417
monomerMeyerozyma guilliermondii, Meyerozyma guilliermondii B10-05, Meyerozyma guilliermondii FTI, Meyerozyma guilliermondii FTI 20037, Meyerozyma guilliermondii H17, Meyerozyma guilliermondii NRRL Y-17257, Meyerozyma guilliermondii OUC1, Meyerozyma guilliermondii PYCC 3012, Meyerozyma guilliermondii UFMG-Y65, Meyerozyma guilliermondii Y-1017-1 * 67000, SDS-PAGE653087
additional informationPenicillium purpurogenum, Penicillium purpurogenum KJS506-? * 23000, SDS-PAGE, AXE II; ? * 48000, SDS-PAGE, AXE I23594
additional informationAspergillus niger-? * 30480, SDS-PAGE23596
additional informationFibrobacter succinogenes, Fibrobacter succinogenes S851, Fibrobacter succinogenes S85-? * 55000, SDS-PAGE23600
additional informationSus scrofa-? * 30000, SDS-PAGE33532
additional informationHypocrea jecorina, Hypocrea jecorina ALKO2877, Hypocrea jecorina CBH I, Hypocrea jecorina Cel6A, Hypocrea jecorina CL847, Hypocrea jecorina FTKO-39, Hypocrea jecorina PC-3-7, Hypocrea jecorina QM6a, Hypocrea jecorina QM 9414, Hypocrea jecorina QM9414, Hypocrea jecorina RUT C-30, Hypocrea jecorina RUT C30, Hypocrea jecorina RUT-C30, Hypocrea jecorina ZU-02-? * 45000, SDS-PAGE35223
additional informationStreptomyces lividans, Streptomyces lividans 66, Streptomyces lividans 66strain 1326, Streptomyces lividans 8, Streptomyces lividans MTCC1, Streptomyces lividans TK64-? * 34000, SDS-PAGE35239

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
glycoproteinAlphacoronavirus, Alphacoronavirus SARS-CoV--652759
glycoproteinHypocrea jecorinaA7J2C6N-glycosylation, sensitive to endoglycosidase H but not to O-glycosidase treatment, putative acceptor sites: N45, N146, N198, N204, N278695758
side-chain modificationSclerotinia sp.-glycoprotein, 3.34% carbohydrate35234
glycoproteinHomo sapiens-residues N160 and N196 are potential sites, in silico analysis696095
additional informationHomo sapiens-residues C140, C149 and C409 with putative intra- and intermolecular disulfide bonds, in silico analysis696095

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
molecular dynamic simulations and automated docking with R and S enantiomers of substrate 1,1,1-trifluoro-2-phenyl-but-3-yn-1-yl acetate using structure of wild-type and structural model of double mutant E188W/M193C, S enantiomer fits better in active site of mutant E188W/M193C because subtrates phenyl group points out of it, docking of the preferred R enantiomer by the wild-type with lower free energy than docking of the S enantiomerBacillus subtilis-695582
His-tagged protein, both in the native form and with selenomethionine substitutionEscherichia coli-663520
structural superimposition of homology-generated model with Alicyclobacillus acidocaldarius EST2 and Escherichia coli beta-cystathionase MalY revealed nine amino acid consensus sequence putatively involved in protein-protein interactions, amino acids 178-184 are putative core of interaction with MalY and MalTEscherichia coli-701087
structural modelling using Cn3D 4.1 software and plant strictosidine synthase as model structure, six-bladed beta-propeller structureHomo sapiens-696095
-Hypocrea jecorina, Hypocrea jecorina ALKO2877, Hypocrea jecorina CBH I, Hypocrea jecorina Cel6A, Hypocrea jecorina CL847, Hypocrea jecorina FTKO-39, Hypocrea jecorina PC-3-7, Hypocrea jecorina QM 9414, Hypocrea jecorina QM6a, Hypocrea jecorina QM9414, Hypocrea jecorina RUT C-30, Hypocrea jecorina RUT C30, Hypocrea jecorina RUT-C30, Hypocrea jecorina ZU-02-23605
-Penicillium purpurogenum, Penicillium purpurogenum KJS506-35238
purified selenomethionine enzyme, 6-7 mg/ml, pure or complexed with the inhibitor dimethylarsinic acid, crystal growth in 1.8 M ammonium sulfate, 0.1 M NaCl, 0.1 M cacodylate, pH 6.5, for crystallization of native methionine enzyme 1.7 M ammonium sulfate, 0.1 M NaCl, 0.1 M BES, pH 6.4, is used, X-ray structure determination and analysis at 1.3 A and 1.45 A resolutionRhodococcus sp., Rhodococcus sp. 19070, Rhodococcus sp. 400, Rhodococcus sp. 7, Rhodococcus sp. A81, Rhodococcus sp. AA21, Rhodococcus sp. AIU Z-35-1, Rhodococcus sp. AJ270, Rhodococcus sp. AK32, Rhodococcus sp. AN-22, Rhodococcus sp. ATCC39484, Rhodococcus sp. B1, Rhodococcus sp. C-x, Rhodococcus sp. C1, Rhodococcus sp. C9, Rhodococcus sp. DK17, Rhodococcus sp. DX-35, Rhodococcus sp. ECU0066, Rhodococcus sp. F2-7-3, Rhodococcus sp. Fp2, Rhodococcus sp. G-74-2, Rhodococcus sp. GK1, Rhodococcus sp. H1, Rhodococcus sp. HR199, Rhodococcus sp. IGT8, Rhodococcus sp. IGTS8, Rhodococcus sp. IOC-77, Rhodococcus sp. J1, Rhodococcus sp. M-750, Rhodococcus sp. M-777, Rhodococcus sp. M4, Rhodococcus sp. N-774, Rhodococcus sp. N186/21, Rhodococcus sp. N595, Rhodococcus sp. N771, Rhodococcus sp. NCIMB 11215, Rhodococcus sp. NCIMB 11216, Rhodococcus sp. NCIMB 12038, Rhodococcus sp. NCIMB 9784, Rhodococcus sp. NCIMB12038, Rhodococcus sp. NDB 1165, Rhodococcus sp. Novo SP361, Rhodococcus sp. P1, Rhodococcus sp. R04, Rhodococcus sp. R312, Rhodococcus sp. RHA1, Rhodococcus sp. S13-4, Rhodococcus sp. SHZ-1, Rhodococcus sp. TK6-652394
-Sclerotinia sp.-35234

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
38Aspergillus niger--23596
59Streptomyces sp.-stable for 30 min701417
69Eleusine coracana-stable680319
11-Aspergillus niger-unstable above35230

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
3040Eleusine coracana-stable680319
37-Alphacoronavirus, Alphacoronavirus SARS-CoV-40 min, the transiently expressed respiratory viruses show significantly reduced activity, while the transiently expressed enteropathogenic viruses are stable652759
4060Kluyveromyces marxianus B57, Kluyveromyces marxianus CBS 1553, Kluyveromyces marxianus CBS6556, Kluyveromyces marxianus CCEBI 2011, Kluyveromyces marxianus, Kluyveromyces marxianus DSM5418, Kluyveromyces marxianus IMB3, Kluyveromyces marxianus NBIMCC 1984, Kluyveromyces marxianus NRRL Y-7571, Kluyveromyces marxianus UNSW 510700, Kluyveromyces marxianus Y-488-decreasing stability, EST1 sample after anion-exchange chromatography, p-nitrophenyl acetate as substrate, pH 7, 1 h699069
40-Aspergillus oryzae-pH 5-6, 24 h stable35241
50-Kluyveromyces marxianus, Kluyveromyces marxianus B57, Kluyveromyces marxianus CBS 1553, Kluyveromyces marxianus CBS6556, Kluyveromyces marxianus CCEBI 2011, Kluyveromyces marxianus DSM5418, Kluyveromyces marxianus IMB3, Kluyveromyces marxianus NBIMCC 1984, Kluyveromyces marxianus NRRL Y-7571, Kluyveromyces marxianus UNSW 510700, Kluyveromyces marxianus Y-488-75% activity, EST1 sample after anion-exchange chromatography, p-nitrophenyl acetate as substrate, pH 7, 1 h699069
60-Meyerozyma guilliermondii B10-05, Meyerozyma guilliermondii FTI 20037, Meyerozyma guilliermondii FTI, Meyerozyma guilliermondii H17, Meyerozyma guilliermondii NRRL Y-17257, Meyerozyma guilliermondii OUC1, Meyerozyma guilliermondii PYCC 3012, Meyerozyma guilliermondii, Meyerozyma guilliermondii UFMG-Y65, Meyerozyma guilliermondii Y-1017-stable up to653087
60-Acinetobacter sp. 15150, Acinetobacter sp., Acinetobacter sp. 210A, Acinetobacter sp. 3B-1, Acinetobacter sp. 4B, Acinetobacter sp. AG, Acinetobacter sp. AK226, Acinetobacter sp. C-17, Acinetobacter sp. C1W, Acinetobacter sp. CGMCC, Acinetobacter sp. CHC, Acinetobacter sp. CHE4-1, Acinetobacter sp. CR9, Acinetobacter sp. CRH-104D, Acinetobacter sp. DV1, Acinetobacter sp. HO1-N, Acinetobacter sp. JCI, Acinetobacter sp. M-1, Acinetobacter sp. M-30, Acinetobacter sp. MTCC 6816, Acinetobacter sp. NCIB, Acinetobacter sp. NCIMB 9871, Acinetobacter sp. SA1, Acinetobacter sp. ST-1, Acinetobacter sp. TD63, Acinetobacter sp. YAA-1 h, 60% residual activity666086
60-Streptomyces sp.-stable for 30 min at temperatures up to 60°C in absence of substrate701417
61-Escherichia coli-denaturation of Aes mutant V20D, thermal stability analysis at 20-90°C, differential scanning calorimetry and circular dichroism measurements, the irreversible inactivation process is more complex than a two-state transition682897
65-Aspergillus niger-unstable above35230
65-Escherichia coli-t1/2 inactivation is 5 min for the wild-type enzyme, and 30 min for mutant T74A, thermal inactivation first-order kinetics, overview682718
68-Escherichia coli-denaturation of Aes, thermal stability analysis at 20-90°C, differential scanning calorimetry and circular dichroism measurements, the irreversible inactivation process is more complex than a two-state transition682897

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
AcetoneKluyveromyces marxianus, Kluyveromyces marxianus B57, Kluyveromyces marxianus CBS 1553, Kluyveromyces marxianus CBS6556, Kluyveromyces marxianus CCEBI 2011, Kluyveromyces marxianus DSM5418, Kluyveromyces marxianus IMB3, Kluyveromyces marxianus NBIMCC 1984, Kluyveromyces marxianus NRRL Y-7571, Kluyveromyces marxianus UNSW 510700, Kluyveromyces marxianus Y-488-15% (v/v), 48% residual activity, EST1, p-nitrophenyl acetate as substrate699069
dimethyl sulfoxideKluyveromyces marxianus, Kluyveromyces marxianus B57, Kluyveromyces marxianus CBS 1553, Kluyveromyces marxianus CBS6556, Kluyveromyces marxianus CCEBI 2011, Kluyveromyces marxianus DSM5418, Kluyveromyces marxianus IMB3, Kluyveromyces marxianus NBIMCC 1984, Kluyveromyces marxianus NRRL Y-7571, Kluyveromyces marxianus UNSW 510700, Kluyveromyces marxianus Y-488-15% (v/v), 39% residual activity, EST1, p-nitrophenyl acetate as substrate699069
dimethylformamideKluyveromyces marxianus, Kluyveromyces marxianus B57, Kluyveromyces marxianus CBS 1553, Kluyveromyces marxianus CBS6556, Kluyveromyces marxianus CCEBI 2011, Kluyveromyces marxianus DSM5418, Kluyveromyces marxianus IMB3, Kluyveromyces marxianus NBIMCC 1984, Kluyveromyces marxianus NRRL Y-7571, Kluyveromyces marxianus UNSW 510700, Kluyveromyces marxianus Y-488-15% (v/v), 34% residual activity, EST1, p-nitrophenyl acetate as substrate699069
EthanolKluyveromyces marxianus, Kluyveromyces marxianus B57, Kluyveromyces marxianus CBS 1553, Kluyveromyces marxianus CBS6556, Kluyveromyces marxianus CCEBI 2011, Kluyveromyces marxianus DSM5418, Kluyveromyces marxianus IMB3, Kluyveromyces marxianus NBIMCC 1984, Kluyveromyces marxianus NRRL Y-7571, Kluyveromyces marxianus UNSW 510700, Kluyveromyces marxianus Y-488-15% (v/v), 76% residual activity, EST1, p-nitrophenyl acetate as substrate699069
MethanolKluyveromyces marxianus, Kluyveromyces marxianus B57, Kluyveromyces marxianus CBS 1553, Kluyveromyces marxianus CBS6556, Kluyveromyces marxianus CCEBI 2011, Kluyveromyces marxianus DSM5418, Kluyveromyces marxianus IMB3, Kluyveromyces marxianus NBIMCC 1984, Kluyveromyces marxianus NRRL Y-7571, Kluyveromyces marxianus UNSW 510700, Kluyveromyces marxianus Y-488-15% (v/v), 82% residual activity, EST1, p-nitrophenyl acetate as substrate699069

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
stabilization by reducing agentsBos taurus, Bos taurus aiiA, Bos taurus CuZn-SOD, Bos taurus PDE6-35224

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
unstableBos taurus-35224
4°C, several monthsNocardia sp., Nocardia sp. 108, Nocardia sp. 239, Nocardia sp. 3CS, Nocardia sp. LSU 169, Nocardia sp. M117, Nocardia sp. NRRL 5646, Nocardia sp. P18.3-35233
stored at -20°C in 20% glycerolStreptomyces sp., Streptomyces sp. 142, Streptomyces sp. 17-1, Streptomyces sp. 174, Streptomyces sp. 2B, Streptomyces sp. 3, Streptomyces sp. 3022a, Streptomyces sp. 594, Streptomyces sp. 65, Streptomyces sp. A19249, Streptomyces sp. A5, Streptomyces sp. AA586, Streptomyces sp. AB3534, Streptomyces sp. AKU 2801, Streptomyces sp. ALG-5, Streptomyces sp. C-248, Streptomyces sp. C-254, Streptomyces sp. Chainia, Streptomyces sp. CL190, Streptomyces sp. CS-57, Streptomyces sp. CS326, Streptomyces sp. E-86, Streptomyces sp. E750-3, Streptomyces sp. FLA, Streptomyces sp. FR-008, Streptomyces sp. G26, Streptomyces sp. GERI-155, Streptomyces sp. IN1, Streptomyces sp. J-13-3, Streptomyces sp. J-350P, Streptomyces sp. J-845S, Streptomyces sp. K11, Streptomyces sp. K37, Streptomyces sp. KB210-8SY, Streptomyces sp. ki-8, Streptomyces sp. KJ-72, Streptomyces sp. KO-3988, Streptomyces sp. KT-23, Streptomyces sp. L-13, Streptomyces sp. L-1689-23, Streptomyces sp. LL-B7, Streptomyces sp. M-03, Streptomyces sp. M-20, Streptomyces sp. M23, Streptomyces sp. MCI-2524, Streptomyces sp. MTCC 7546, Streptomyces sp. NCIM 2730, Streptomyces sp. NCIM 5127, Streptomyces sp. NL15-2K, Streptomyces sp. No. 3137, Streptomyces sp. No. 6, Streptomyces sp. NRRL 5331, Streptomyces sp. OH-11242, Streptomyces sp. OH11242, Streptomyces sp. PCB7, Streptomyces sp. R-39, Streptomyces sp. R39, Streptomyces sp. RC 1071, Streptomyces sp. S38, Streptomyces sp. SA-COO, Streptomyces sp. SCC-2136, Streptomyces sp. strainTH1, Streptomyces sp. T109-3, Streptomyces sp. TH-4, Streptomyces sp. TH1, Streptomyces sp. UC5319, Streptomyces sp. WM-23, Streptomyces sp. X9, Streptomyces sp. Z-11-6-701417

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
partialAcinetobacter sp., Acinetobacter sp. 15150, Acinetobacter sp. 210A, Acinetobacter sp. 3B-1, Acinetobacter sp. 4B, Acinetobacter sp. AG, Acinetobacter sp. AK226, Acinetobacter sp. C-17, Acinetobacter sp. C1W, Acinetobacter sp. CGMCC, Acinetobacter sp. CHC, Acinetobacter sp. CHE4-1, Acinetobacter sp. CR9, Acinetobacter sp. CRH-104D, Acinetobacter sp. DV1, Acinetobacter sp. HO1-N, Acinetobacter sp. JCI, Acinetobacter sp. M-1, Acinetobacter sp. M-30, Acinetobacter sp. MTCC 6816, Acinetobacter sp. NCIB, Acinetobacter sp. NCIMB 9871, Acinetobacter sp. SA1, Acinetobacter sp. ST-1, Acinetobacter sp. TD63, Acinetobacter sp. YAA-666086
purification of virus tissue material, nasal swab and from COS-7 cellsAlphacoronavirus, Alphacoronavirus SARS-CoV-652759
-Antheraea polyphemus-35226
-Aspergillus niger-23596, 35230, 35244
from the crude commercial enzyme preparation, 27foldAspergillus niger-650815
-Aspergillus oryzae-35241
native enzyme to homogeneity by SDS-PAGE and isoelectric focusingAspergillus oryzae-680967
-Bacillus pumilus, Bacillus pumilus 76, Bacillus pumilus A8, Bacillus pumilus B26, Bacillus pumilus BK2, Bacillus pumilus Bn-262, Bacillus pumilus CECT 5072, Bacillus pumilus DKS1, Bacillus pumilus HL721, Bacillus pumilus IPO, Bacillus pumilus P9, Bacillus pumilus PS213, Bacillus pumilus PS214-23597
-Bacillus subtilis-35235
-Bos taurus-35224
-Candida bororiensis-35232
immobilized metal affinity chromatographyCellvibrio japonicus, Cellvibrio japonicus NCIMB 10462-715029
single-step purificationCitrus sinensis-663817
immobilized metal affinity chromatographyClostridium thermocellum, Clostridium thermocellum 27405., Clostridium thermocellum 651, Clostridium thermocellum ATCC27405, Clostridium thermocellum CBH III, Clostridium thermocellum CelJ, Clostridium thermocellum F7, Clostridium thermocellum YM4, Clostridium thermocellum ZJL4-715029
partial, separation of isoenzymesCymbopogon martinii-653450
-Drosophila mojavensis-35228
native enzyme from 72 h ragi malt 34fold by ammonium sulfate precipitation, anion exchange chromatography, gel filtration, and hydrophobic interaction chromatography, to homogeneityEleusine coracana-680319
from bacterial extracts by binding to CNBr-activated Sepharose CL6B, 1.5 mg bound protein per ml resin, pH 8.3, 0.1 M sodium hydrogen carbonate, 0.5 M sodium chlorideEscherichia coli-701087
His-tagged proteinEscherichia coli-663520
recombinant wild-type and mutant enzymes from strain BL21(DE3) by anion exchange and hydrophobic interaction chromatography, and gel filtrationEscherichia coli-682718
-Fibrobacter succinogenes, Fibrobacter succinogenes S85, Fibrobacter succinogenes S851-23600
from bacterial lysate or inclusion bodies by Superose 6 column chromatography in presence of 6-8 M urea for antibody generation, from insect cells by FPLC-based metal affinity chromatography (250 mM imidazole) and anion-exchange chromatography on Q-sepharose column for activity measurementsHomo sapiens-696095
-Hypocrea jecorina-35223
after over-expression (up to 20fold activity) by liquid column chromatography, monitored by activity measurements, 300 mg recombinant enzyme from 1 litre culture; centrifugation at 4°C, liquid column chromatographyHypocrea jecorinaA7J2C6695758
-Hypocrea jecorina ALKO2877, Hypocrea jecorina CBH I, Hypocrea jecorina Cel6A, Hypocrea jecorina CL847, Hypocrea jecorina FTKO-39, Hypocrea jecorina PC-3-7, Hypocrea jecorina QM 9414, Hypocrea jecorina QM6a, Hypocrea jecorina QM9414, Hypocrea jecorina RUT C-30, Hypocrea jecorina RUT C30, Hypocrea jecorina RUT-C30, Hypocrea jecorina ZU-02-35223
85% (NH4)2SO4-saturated bacterial protein extract to anion-exchange chromatography on DEAE-650 (S) column (gradient elution with 0-0.15 M NaCl), EST1 elution at 70 mM NaCl, EST2 elution at 90 mM NaCl, addition of (NH4)2SO4 (25% saturation) to EST1 fraction followed by hydrophobic interaction chromatography on phenyl Sepharose column, purification of main esterase activity (EST1) by elution with 10% (NH4)2SO4-saturated buffer, preparative electrophoresis (native)Kluyveromyces marxianus, Kluyveromyces marxianus B57, Kluyveromyces marxianus CBS 1553, Kluyveromyces marxianus CBS6556, Kluyveromyces marxianus CCEBI 2011, Kluyveromyces marxianus DSM5418, Kluyveromyces marxianus IMB3, Kluyveromyces marxianus NBIMCC 1984, Kluyveromyces marxianus NRRL Y-7571, Kluyveromyces marxianus UNSW 510700, Kluyveromyces marxianus Y-488-699069
-Lactobacillus plantarum-35236
isoenzymes Est I and Est IIMarchantia polymorpha-666089
8.6fold, to homogeneityMeyerozyma guilliermondii, Meyerozyma guilliermondii B10-05, Meyerozyma guilliermondii FTI, Meyerozyma guilliermondii FTI 20037, Meyerozyma guilliermondii H17, Meyerozyma guilliermondii NRRL Y-17257, Meyerozyma guilliermondii OUC1, Meyerozyma guilliermondii PYCC 3012, Meyerozyma guilliermondii UFMG-Y65, Meyerozyma guilliermondii Y-1017-653087
-Nocardia sp., Nocardia sp. 108, Nocardia sp. 239, Nocardia sp. 3CS, Nocardia sp. LSU 169, Nocardia sp. M117, Nocardia sp. NRRL 5646, Nocardia sp. P18.3-35233
-Orpinomyces sp., Orpinomyces sp. KF7-35242
two enzymes: AXE I and AXE IIPenicillium purpurogenum, Penicillium purpurogenum KJS506-23594
-Petroselinum crispum-35199
recombinant from Escherichia coli as N-terminally His-tagged selenomethionine or methionine enzyme, removal of the His-tagRhodococcus sp., Rhodococcus sp. 19070, Rhodococcus sp. 400, Rhodococcus sp. 7, Rhodococcus sp. A81, Rhodococcus sp. AA21, Rhodococcus sp. AIU Z-35-1, Rhodococcus sp. AJ270, Rhodococcus sp. AK32, Rhodococcus sp. AN-22, Rhodococcus sp. ATCC39484, Rhodococcus sp. B1, Rhodococcus sp. C-x, Rhodococcus sp. C1, Rhodococcus sp. C9, Rhodococcus sp. DK17, Rhodococcus sp. DX-35, Rhodococcus sp. ECU0066, Rhodococcus sp. F2-7-3, Rhodococcus sp. Fp2, Rhodococcus sp. G-74-2, Rhodococcus sp. GK1, Rhodococcus sp. H1, Rhodococcus sp. HR199, Rhodococcus sp. IGT8, Rhodococcus sp. IGTS8, Rhodococcus sp. IOC-77, Rhodococcus sp. J1, Rhodococcus sp. M-750, Rhodococcus sp. M-777, Rhodococcus sp. M4, Rhodococcus sp. N-774, Rhodococcus sp. N186/21, Rhodococcus sp. N595, Rhodococcus sp. N771, Rhodococcus sp. NCIMB 11215, Rhodococcus sp. NCIMB 11216, Rhodococcus sp. NCIMB 12038, Rhodococcus sp. NCIMB 9784, Rhodococcus sp. NCIMB12038, Rhodococcus sp. NDB 1165, Rhodococcus sp. Novo SP361, Rhodococcus sp. P1, Rhodococcus sp. R04, Rhodococcus sp. R312, Rhodococcus sp. RHA1, Rhodococcus sp. S13-4, Rhodococcus sp. SHZ-1, Rhodococcus sp. TK6-652394
-Rhodotorula mucilaginosa, Rhodotorula mucilaginosa NRC 211003, Rhodotorula mucilaginosa PRL251-23601
-Streptomyces lividans, Streptomyces lividans 66, Streptomyces lividans 66strain 1326, Streptomyces lividans 8, Streptomyces lividans MTCC1, Streptomyces lividans TK64-35239
-Streptomyces olivochromogenes, Streptomyces olivochromogenes IFO3178, Streptomyces olivochromogenes NRCC 2258-35193
at 4°C, precipitation from culture filtrate by 40-80% (NH4)2SO4 saturation followed by anion-exchange (DEAE column, elution with 0-1 M NaCl gradient), t-butyl hydrophobic (elution with 1.7-0 M (NH4)2SO4 gradient) and hydroxyapatite chromatography (elution with 0.02-0.5 M sodium phosphate buffer, pH 6.8), stored at -20°C after addition of 20% glycerolStreptomyces sp.-701417
-Sus scrofa-33532

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
respiratory and enteropathogenic coronaviruses, DNA and amino acid sequence determination and analysis, transient and functional expression in COS-7 cellsAlphacoronavirus, Alphacoronavirus SARS-CoV-652759
expressed in Escherichia coli BL21(DE3) cellsCellvibrio japonicus, Cellvibrio japonicus NCIMB 10462, Clostridium thermocellum, Clostridium thermocellum 27405., Clostridium thermocellum 651, Clostridium thermocellum ATCC27405, Clostridium thermocellum CBH III, Clostridium thermocellum CelJ, Clostridium thermocellum F7, Clostridium thermocellum YM4, Clostridium thermocellum ZJL4-715029
expressed in Escherichia coli JM109 cellsEscherichia coliP23872715950
gene ybaC, overexpression of wild-type and mutant enzymesEscherichia coli-682897
overexpression of wild-type and mutant enzymes in strain BL21(DE3)Escherichia coli-682718
cDNA of 1248 bp in pGEM-T-easy for sequencing (polymorphic residues at position 65, 282, 374, in middle European population homozygosity for R282 and R347) and subcloning, cDNA coding for residues 45-416 in pDEST15 or residues 58-416 in pGEX-1gammaT (yielding glutathione transferase-tag) for expression in Escherichia coli BL21, cDNA coding for residues 16-416 in pDEST10 for baculovirus-mediated expression in insect cell lines Sf9 and HighFive with N-terminal hexa-His-tagHomo sapiens-696095
expression in Escherichia coli DH5 alpha; from genomic DNA in pT3C to generate expression plasmid pTSP-TAE and transformation of Hypocrea jecorina Rut-C30Hypocrea jecorinaA7J2C6695758
expression in Escherichia coliOrpinomyces sp., Orpinomyces sp. KF7-35242
expression of the N-terminally His-tagged enzyme in Escherichia coli BL21(DE3)Rhodococcus sp., Rhodococcus sp. 19070, Rhodococcus sp. 400, Rhodococcus sp. 7, Rhodococcus sp. A81, Rhodococcus sp. AA21, Rhodococcus sp. AIU Z-35-1, Rhodococcus sp. AJ270, Rhodococcus sp. AK32, Rhodococcus sp. AN-22, Rhodococcus sp. ATCC39484, Rhodococcus sp. B1, Rhodococcus sp. C-x, Rhodococcus sp. C1, Rhodococcus sp. C9, Rhodococcus sp. DK17, Rhodococcus sp. DX-35, Rhodococcus sp. ECU0066, Rhodococcus sp. F2-7-3, Rhodococcus sp. Fp2, Rhodococcus sp. G-74-2, Rhodococcus sp. GK1, Rhodococcus sp. H1, Rhodococcus sp. HR199, Rhodococcus sp. IGT8, Rhodococcus sp. IGTS8, Rhodococcus sp. IOC-77, Rhodococcus sp. J1, Rhodococcus sp. M-750, Rhodococcus sp. M-777, Rhodococcus sp. M4, Rhodococcus sp. N-774, Rhodococcus sp. N186/21, Rhodococcus sp. N595, Rhodococcus sp. N771, Rhodococcus sp. NCIMB 11215, Rhodococcus sp. NCIMB 11216, Rhodococcus sp. NCIMB 12038, Rhodococcus sp. NCIMB 9784, Rhodococcus sp. NCIMB12038, Rhodococcus sp. NDB 1165, Rhodococcus sp. Novo SP361, Rhodococcus sp. P1, Rhodococcus sp. R04, Rhodococcus sp. R312, Rhodococcus sp. RHA1, Rhodococcus sp. S13-4, Rhodococcus sp. SHZ-1, Rhodococcus sp. TK6-652394
overexpression in Streptomyces lividans IAF10-164Streptomyces lividans, Streptomyces lividans 66, Streptomyces lividans 66strain 1326, Streptomyces lividans 8, Streptomyces lividans MTCC1, Streptomyces lividans TK64-35239

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
E188DBacillus subtilis-very low specific activity, R enantioselectivity of above 100, S enantioselectivity of 26, mutant generated using a focused directed-evolution approach based on saturation mutagenesis according to CASTing method695582
E188WBacillus subtilis-very low specific activity similar to mutant M193C, S enantioselectivity of 26695582
E188W/M193CBacillus subtilis-14% of wild-type specific activity, wider substrate range than wild-type with respect to tertiary alcohol acetates, inversed enantioselectivity, S enantioselectivity of 64, binding of S enantiomer of substrate 1,1,1-trifluoro-2-phenyl-but-3-yn-1-yl acetate with lower free energy than wild-type, M193C mutation seems to stabilize S orientation, mutant generated using a focused directed-evolution approach based on saturation mutagenesis according to CASTing method695582
R179AEscherichia coli-mutation in interaction consensus sequence, wild-type activity, loss of interaction with Aes interactors such as MalY701087
R48AEscherichia coliP23872the enzymatic activity of the mutant is enhanced by improvement of catalytic efficiency (3.7fold with 4-nitrophenyl butyrate and 8fold with tributyrin as substrate)715950
R48EEscherichia coliP23872the enzymatic activity of the mutant is enhanced by improvement of catalytic efficiency (3.4fold with 4-nitrophenyl butyrate and 1.3fold with tributyrin as substrate)715950
R48KEscherichia coliP23872the catalytic efficiency of the mutant is similar to the wild type enzyme715950
R48SEscherichia coliP23872the enzymatic activity of the mutant is enhanced by improvement of catalytic efficiency (2.6fold with 4-nitrophenyl butyrate and 9.7fold with tributyrin as substrate)715950
T74AEscherichia coli-random mutagenesis, the mutant shows increased thermostability compared to the wild-type enzyme682718
M193CBacillus subtilis-very low specific activity similar to mutant E188W, R enantioselectivity of 16695582
additional informationEscherichia coli-random mutagenesis and screening for thermostable mutants at 85°C682718
V20DEscherichia coli-site-directed mutagenesis, the mutant shows slightly reduced thermal stability compared to the wild-type enzyme, kinetic analysis, overview682897
additional informationRhodococcus sp., Rhodococcus sp. 19070, Rhodococcus sp. 400, Rhodococcus sp. 7, Rhodococcus sp. A81, Rhodococcus sp. AA21, Rhodococcus sp. AIU Z-35-1, Rhodococcus sp. AJ270, Rhodococcus sp. AK32, Rhodococcus sp. AN-22, Rhodococcus sp. ATCC39484, Rhodococcus sp. B1, Rhodococcus sp. C-x, Rhodococcus sp. C1, Rhodococcus sp. C9, Rhodococcus sp. DK17, Rhodococcus sp. DX-35, Rhodococcus sp. ECU0066, Rhodococcus sp. F2-7-3, Rhodococcus sp. Fp2, Rhodococcus sp. G-74-2, Rhodococcus sp. GK1, Rhodococcus sp. H1, Rhodococcus sp. HR199, Rhodococcus sp. IGT8, Rhodococcus sp. IGTS8, Rhodococcus sp. IOC-77, Rhodococcus sp. J1, Rhodococcus sp. M-750, Rhodococcus sp. M-777, Rhodococcus sp. M4, Rhodococcus sp. N-774, Rhodococcus sp. N186/21, Rhodococcus sp. N595, Rhodococcus sp. N771, Rhodococcus sp. NCIMB 11215, Rhodococcus sp. NCIMB 11216, Rhodococcus sp. NCIMB 12038, Rhodococcus sp. NCIMB 9784, Rhodococcus sp. NCIMB12038, Rhodococcus sp. NDB 1165, Rhodococcus sp. Novo SP361, Rhodococcus sp. P1, Rhodococcus sp. R04, Rhodococcus sp. R312, Rhodococcus sp. RHA1, Rhodococcus sp. S13-4, Rhodococcus sp. SHZ-1, Rhodococcus sp. TK6-construction of selenomethionine enzyme by growth of the recombinant methionine auxotroph Escherichia coli strain B834-(DE3) expressing the enzyme in minimal medium containing selenomethionine652394

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
reversible unfolding of enzyme with both urea and guanidinium-HCl. Unfolding data suggest the presence of two domains which unfold more or less independentlyEscherichia coli-664019

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
synthesisCymbopogon martinii-enzyme is useful for the commercial geraniol production from palmarosa oil653450
molecular biologyEscherichia coli-protein-protein interaction consensus sequence, involved in regulation of both sugar and lipid metabolism, according to interaction partners701087
molecular biologyHomo sapiens-postulated PON (paraoxonase) family membership due to similarity to primary structure of PON2, plant strictosidine synthase and di-isopropyl fluorophosphatase, its N-terminal single transmembrane domain, a nine-exon gene structure, a six-bladed beta-propeller tertiary structure, and similar metabolic regulation of gene expression696095
industryKluyveromyces marxianus, Kluyveromyces marxianus B57, Kluyveromyces marxianus CBS 1553, Kluyveromyces marxianus CBS6556, Kluyveromyces marxianus CCEBI 2011, Kluyveromyces marxianus DSM5418, Kluyveromyces marxianus IMB3, Kluyveromyces marxianus NBIMCC 1984, Kluyveromyces marxianus NRRL Y-7571, Kluyveromyces marxianus UNSW 510700, Kluyveromyces marxianus Y-488-use of the very active biocatalyst EST1 for chiral resolution of 1,2-O-isopropylidene glycol esters699069
paper productionMeyerozyma guilliermondii, Meyerozyma guilliermondii B10-05, Meyerozyma guilliermondii FTI, Meyerozyma guilliermondii FTI 20037, Meyerozyma guilliermondii H17, Meyerozyma guilliermondii NRRL Y-17257, Meyerozyma guilliermondii OUC1, Meyerozyma guilliermondii PYCC 3012, Meyerozyma guilliermondii UFMG-Y65, Meyerozyma guilliermondii Y-1017-the enzyme can be a potential source for pretreatment of pulps and biobleaching in paper manufacture due to its activity with acetylated xylan leading to complete hydrolysis653087
analysisRhodococcus sp., Rhodococcus sp. 19070, Rhodococcus sp. 400, Rhodococcus sp. 7, Rhodococcus sp. A81, Rhodococcus sp. AA21, Rhodococcus sp. AIU Z-35-1, Rhodococcus sp. AJ270, Rhodococcus sp. AK32, Rhodococcus sp. AN-22, Rhodococcus sp. ATCC39484, Rhodococcus sp. B1, Rhodococcus sp. C-x, Rhodococcus sp. C1, Rhodococcus sp. C9, Rhodococcus sp. DK17, Rhodococcus sp. DX-35, Rhodococcus sp. ECU0066, Rhodococcus sp. F2-7-3, Rhodococcus sp. Fp2, Rhodococcus sp. G-74-2, Rhodococcus sp. GK1, Rhodococcus sp. H1, Rhodococcus sp. HR199, Rhodococcus sp. IGT8, Rhodococcus sp. IGTS8, Rhodococcus sp. IOC-77, Rhodococcus sp. J1, Rhodococcus sp. M-750, Rhodococcus sp. M-777, Rhodococcus sp. M4, Rhodococcus sp. N-774, Rhodococcus sp. N186/21, Rhodococcus sp. N595, Rhodococcus sp. N771, Rhodococcus sp. NCIMB 11215, Rhodococcus sp. NCIMB 11216, Rhodococcus sp. NCIMB 12038, Rhodococcus sp. NCIMB 9784, Rhodococcus sp. NCIMB12038, Rhodococcus sp. NDB 1165, Rhodococcus sp. Novo SP361, Rhodococcus sp. P1, Rhodococcus sp. R04, Rhodococcus sp. R312, Rhodococcus sp. RHA1, Rhodococcus sp. S13-4, Rhodococcus sp. SHZ-1, Rhodococcus sp. TK6-development of a heroin biosensor with high sensitivity652394
industrySaccharomyces cerevisiae-enhancement of sensory quality in alcoholic beverage through higher activity of Saccharomyces cerevisiae EAHase in mixed culture with Pichia anomala mutant with respiration deficiency698520
industryStreptomyces sp., Streptomyces sp. 142, Streptomyces sp. 17-1, Streptomyces sp. 174, Streptomyces sp. 2B, Streptomyces sp. 3, Streptomyces sp. 3022a, Streptomyces sp. 594, Streptomyces sp. 65, Streptomyces sp. A19249, Streptomyces sp. A5, Streptomyces sp. AA586, Streptomyces sp. AB3534, Streptomyces sp. AKU 2801, Streptomyces sp. ALG-5, Streptomyces sp. C-248, Streptomyces sp. C-254, Streptomyces sp. Chainia, Streptomyces sp. CL190, Streptomyces sp. CS-57, Streptomyces sp. CS326, Streptomyces sp. E-86, Streptomyces sp. E750-3, Streptomyces sp. FLA, Streptomyces sp. FR-008, Streptomyces sp. G26, Streptomyces sp. GERI-155, Streptomyces sp. IN1, Streptomyces sp. J-13-3, Streptomyces sp. J-350P, Streptomyces sp. J-845S, Streptomyces sp. K11, Streptomyces sp. K37, Streptomyces sp. KB210-8SY, Streptomyces sp. ki-8, Streptomyces sp. KJ-72, Streptomyces sp. KO-3988, Streptomyces sp. KT-23, Streptomyces sp. L-13, Streptomyces sp. L-1689-23, Streptomyces sp. LL-B7, Streptomyces sp. M-03, Streptomyces sp. M-20, Streptomyces sp. M23, Streptomyces sp. MCI-2524, Streptomyces sp. MTCC 7546, Streptomyces sp. NCIM 2730, Streptomyces sp. NCIM 5127, Streptomyces sp. NL15-2K, Streptomyces sp. No. 3137, Streptomyces sp. No. 6, Streptomyces sp. NRRL 5331, Streptomyces sp. OH-11242, Streptomyces sp. OH11242, Streptomyces sp. PCB7, Streptomyces sp. R-39, Streptomyces sp. R39, Streptomyces sp. RC 1071, Streptomyces sp. S38, Streptomyces sp. SA-COO, Streptomyces sp. SCC-2136, Streptomyces sp. strainTH1, Streptomyces sp. T109-3, Streptomyces sp. TH-4, Streptomyces sp. TH1, Streptomyces sp. UC5319, Streptomyces sp. WM-23, Streptomyces sp. X9, Streptomyces sp. Z-11-6-cooperative action on birchwood xylan degradation in combination with Streptomyces sp. xylanase and beta xylosidase, production of biofuels utilizing hemicelluloses containing acetyl xylan, 1.16fold to 1.39fold increased sugar release701417
industryWickerhamomyces anomalus, Wickerhamomyces anomalus AL112, Wickerhamomyces anomalus K, Wickerhamomyces anomalus NCYC 434, Wickerhamomyces anomalus SL-125-enhancement of sensory quality in alcoholic beverage through higher activity of Saccharomyces cerevisiae EAHase in mixed culture with Pichia anomala low respirating mutant698520

DISEASETITLE OF PUBLICATIONLINK TO PUBMED
Addison Disease- PubMed
Anemia- PubMed
Autoimmune Diseases- PubMed,  PubMed
Cysts- PubMed
Diabetes Mellitus- PubMed
Diabetes Mellitus, Type 1- PubMed
Encephalomyelitis- PubMed
Graves Disease- PubMed
Hepatitis- PubMed
Infection- PubMed
Influenza, Human- PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed,  PubMed
Leukemia- PubMed
Liver Cirrhosis, Biliary- PubMed
Lymphoma, B-Cell- PubMed
Nematode Infections- PubMed
Pre-Eclampsia- PubMed
Sarcoidosis- PubMed
Vaccinia- PubMed,  PubMed,  PubMed

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISM (UNIPROT ACCESSION NO.)LINK TO PUBMEDSOURCE
23594Egana, L.; Gutierrez, R.; Caputo, V.; Peirano, A.; Steiner, J.; Eyzaguirre, J.Purification and characterization of two acetyl xylan esterases from Penicillium purpurogenumBiotechnol. Appl. Biochem.2433-391996Penicillium purpurogenum PubMed
23596Kormelink, F.J.M.; Lefebvre, B.; Strozyk, F.; Voragen, A.G.J.Purification and characterization of an acetyl xylan esterase from Aspergillus nigerJ. Biotechnol.27267-2821993Aspergillus niger-
23597Degrassi, G.; Okeke, B.C.; Bruschi, C.V.; Venturi, V.Purification and characterization of an acetyl xylan esterase from Bacillus pumilusAppl. Environ. Microbiol.64789-7921998Bacillus pumilus PubMed
23600McDermid, K.P.; Forsberg, C.W.; MacKenzie, C.R.Purification and properties of an acetylxylan esterase from Fibrobacter succinogenes S85Appl. Environ. Microbiol.563805-38101990Fibrobacter succinogenes, Fibrobacter succinogenes S85 PubMed
23601Lee, H.; To, R.J.B.; Latta, R.K.; Biely, P.; Schneider, H.Some properties of extracellular acetylxylan esterase produced by the yeast Rhodotorula mucilaginosaAppl. Environ. Microbiol.532831-28341987Rhodotorula mucilaginosa PubMed
23605Hakulinen, N.; Tenkanen, M.; Rouvinen, J.Crystallization and preliminary X-ray diffraction studies of the catalytic core of acetyl xylan esterase from Trichoderma reeseiActa Crystallogr. Sect. D54430-4321998Hypocrea jecorina PubMed
23607Biely, P.; MacKenzie, C.R.; Schneider, H.Acetylxylan esterase of Schizophyllum communeMethods Enzymol.160700-7071988Schizophyllum commune-
33532Pullan, L.M.; Noltmann, E.A.Purification and properties of pig muscle carbonic anhydrase IIIBiochim. Biophys. Acta839147-1541985Sus scrofa PubMed
35193Johnson, K.G.; Fontana, J.D.; MacKenzie, C.R.Measurement of acetylxylan esterase in StreptomycesMethods Enzymol.160551-5601988Streptomyces olivochromogenes-
35199Matern, U.Acylhydrolases from parsley (Petroselinum hortense). Relative distribution and properties of four esterases hydrolyzing malonic acid hemiesters of flavonoid glucosidesArch. Biochem. Biophys.224261-2711983Petroselinum crispum PubMed
35223Poutanen, K.; Sundberg, M.An acetyl esterase of Trichoderma reesei and its role in the hydrolysis of acetyl xylansAppl. Microbiol. Biotechnol.28419-4241988Hypocrea jecorina-
35224Arav, R.; Rimon, S.Purification and characterization of acetyl esterase from bull testesBiochim. Biophys. Acta916313-3201987Bos taurus PubMed
35226Vogt, R.G.; Riddiford, L.M.; Prestwich, G.D.Kinetic properties of a sex pheromone-degrading enzyme: the sensillar esterase of Antheraea polyphemusProc. Natl. Acad. Sci. USA828827-88311985Antheraea polyphemus PubMed
35228Pen, J.; Rongen, H.A.H.; Beintema, J.J.Purification and properties of esterase-4 from Drosophila mojavensisBiochim. Biophys. Acta789203-2091984Drosophila mojavensis-
35230Schoebel, B.; Pollmann, W.Isolierung und Charakterisierung einer Acetylester-Hydrolase aus Aspergillus nigerZ. Naturforsch. C35696-6981980Aspergillus niger-
35231Steiger, R.F.; Opperdoes, F.R.; Bontemps, J.Subcellular fractionation of Trypanosoma brucei bloodstream forms with special reference to hydrolasesEur. J. Biochem.105163-1751980Trypanosoma brucei PubMed
35232Bucholtz, M.L.; Light, R.J.Hydrolysis of 13-sophorosyloxydocosanoic acid esters by acetyl- and carboxylesterases isolated from Candida bororiensisJ. Biol. Chem.251431-4371976Candida bororiensis PubMed
35233Eubanks, E.F.; Forney, F.W.; Larson, A.D.Purification and characterization of the nocardial acetylesterase involved in 2-butanone degradationJ. Bacteriol.1201133-11431974Nocardia sp., Nocardia sp. LSU 169 PubMed
35234Oi, S.Physical and chemical properties of crystalline acetylesterase from Sclerotinia fungusAgric. Biol. Chem.38809-8161974Sclerotinia sp.-
35235Higerd, T.B.; Spizizen, J.Isolation of two acetyl esterases from extracts of Bacillus subtilisJ. Bacteriol.1141184-11921973Bacillus subtilis PubMed
35236Oterholm, A.; Witter, L.D.; Ordal, Z.J.Purification and properties of an acetyl ester hydrolase (acetylesterase) from Lactobacillus plantarumJ. Dairy Sci.558-131972Lactobacillus plantarum-
35238Pangborn, W.; Erman, M.; Li, N.; Burkhart, B.M.; Pletnev, V.Z.; Duax, W.L.; Gutierrez, R.; Peirano, A.; Eyzaguirre, J.; Thiel, D.J.; Ghosh, D.Characterization of crystals of Penicillium purpurogenum acetyl xylan esterase from high-resolution x-ray diffractionProteins24523-5241996Penicillium purpurogenum PubMed
35239Dupont, C.; Daigneault, N.; Shareck, F.; Morosoli, R.; Kluepfel, D.Purification and characterization of an acetyl xylan esterase produced by Streptomyces lividansBiochem. J.319881-8861996Streptomyces lividans PubMed
35241Tenkanen, M.; Thornton, J.; Viikari, L.An acetylglucomannan esterase of Aspergillus oryzae; purification, characterization and role in the hydrolysis of O-acetyl-galactoglucomannanJ. Biotechnol.42197-2061995Aspergillus oryzae PubMed
35242Blum, D.L.; Li, X.L.; Chen, H.; Ljungdahl, L.G.Characterization of an acetyl xylan esterase from the anaerobic fungus Orpinomyces sp. strain PC-2Appl. Environ. Microbiol.653990-39951999Orpinomyces sp., Orpinomyces sp. (O59869) PubMed
35244Linden, J.; Samara, M.; Decker, S.; Johnson, E.; Boyer, M.; Pecs, M.; Adney, W.; Himmel, M.Purification and characterization of an acetyl esterase from Aspergillus nigerAppl. Biochem. Biotechnol.45383-3931994Aspergillus niger PubMed
650815Altaner, C.; Saake, B.; Puls, J.Specificity of an Aspergillus niger esterase deacetylating cellulose acetateCellulose1085-952003Aspergillus niger-
652394Zhu, X.; Larsen, N.A.; Basran, A.; Bruce, N.C.; Wilson, I.A.Observation of an arsenic adduct in an acetyl esterase crystal structureJ. Biol. Chem.2782008-20142003Rhodococcus sp., Rhodococcus sp. H1 PubMed
652759Lin, X.Q.; Chouljenko, V.N.; Kousoulas, K.G.; Storz, J.Temperature-sensitive acetylesterase activity of haemagglutinin-esterase specified by respiratory bovine coronavirusesJ. Med. Microbiol.491119-11272000Alphacoronavirus PubMed
653087Basaran, P.; Hang, Y.D.Purification and characterization of acetyl esterase from Candida guilliermondiiLett. Appl. Microbiol.30167-1712000Meyerozyma guilliermondii, Meyerozyma guilliermondii NRRL Y-17257 PubMed
653450Dubey, V.S.; Bhalla, R.; Luthra, R.An esterase is involved in geraniol production during palmarosa inflorescence developmentPhytochemistry63257-2642003Cymbopogon martinii PubMed
663520Gerber, K.; Schiefner, A.; Seige, P.; Diederichs, K.; Boos, W.; Welte, W.Crystallization and preliminary x-ray analysis of Aes, an acetyl-esterase from Escherichia coliActa Crystallogr. Sect. DD60531-5332004Escherichia coli PubMed
663817Pasta, P.; Verga, R.; Zambianchi, F.; Daminati, M.Acetyl esterase from Mediterranean oranges: Partial purification, immobilization and biotransformationsBiocatal. Biotransform.22221-2242004Citrus sinensis-
664019Del Vecchio, P.; Graziano, G.; Granata, V.; Farias, T.; Barone, G.; Mandrich, L.; Rossi, M.; Manco, G.Denaturant-induced unfolding of the acetyl-esterase from Escherichia coliBiochemistry4314637-146432004Escherichia coli PubMed
665872Thonar, C.; Liners, F.; Van Cutsem, P.Polymorphism and modulation of cell wall esterase enzyme activities in the chicory root during the growing seasonJ. Exp. Bot.5781-892006Cichorium intybus PubMed
666086Qian, J.H.; Xu, J.H.Catalytic performance of a highly enantioselective (R)-ester hydrolase from a new isolate Acinetobacter sp. CGMCC 0789J. Mol. Catal. B27227-2322004Acinetobacter sp., Acinetobacter sp. CGMCC-
666089Shimoda, K.; Kubota, N.; Hirata, T.; Kawano, T.Asymmetric transformation of enol acetates with esterases from Marchantia polymorphaJ. Mol. Catal. B29123-1272004Marchantia polymorpha-
666091Kremnicky, L.; Mastihuba, V.; Cote, G.L.Trichoderma reesei acetyl esterase catalyzes transesterification in waterJ. Mol. Catal. B30229-2392004Hypocrea jecorina-
678012Orasmo, G.R.; Oliveira-Collet, S.A.; Lapenta, A.S.; de Fatima P. S. Machado, M.Biochemical and genetic polymorphisms for carboxylesterase and acetylesterase in grape clones of Vitis vinifera L. (Vitaceae) cultivarsBiochem. Genet.45663-6702007Vitis vinifera PubMed
680319Latha, G.M.; Muralikrishna, G.Purification and partial characterization of acetic acid esterase from malted finger millet (Eleusine coracana, Indaf-15)J. Agric. Food Chem.55895-9022007Eleusine coracana PubMed
680967Puchart, V.; Vrsanska, M.; Mastihubova, M.; Topakas, E.; Vafiadi, C.; Faulds, C.B.; Tenkanen, M.; Christakopoulos, P.; Biely, P.Substrate and positional specificity of feruloyl esterases for monoferuloylated and monoacetylated 4-nitrophenyl glycosidesJ. Biotechnol.127235-2432007Aspergillus oryzae PubMed
682718Farias, T.; Mandrich, L.; Rossi, M.; Manco, G.Biochemical and thermostability features of acetyl esterase Aes from Escherichia coliProtein Pept. Lett.14165-1692007Escherichia coli PubMed
682897Del Vecchio, P.; Graziano, G.; Barone, G.; Mandrich, L.; Rossi, M.; Manco, G.Temperature-induced denaturation of Aes acetyl-esterase from Escherichia coliThermochim. Acta441144-1492006Escherichia coli-
695582Bartsch, S.; Kourist, R.; Bornscheuer, U.T.Complete inversion of enantioselectivity towards acetylated tertiary alcohols by a double mutant of a Bacillus subtilis esteraseAngew. Chem. Int. Ed.471508-15112008Bacillus subtilis PubMed
695758Li, X.L.; Skory, C.D.; Cotta, M.A.; Puchart, V.; Biely, P.Novel family of carbohydrate esterases, based on identification of the Hypocrea jecorina acetyl esterase geneAppl. Environ. Microbiol.747482-74892008Hypocrea jecorina (A7J2C6), Hypocrea jecorina PubMed
696095Ilhan, A.; Gartner, W.; Nabokikh, A.; Daneva, T.; Majdic, O.; Cohen, G.; Boehmig, G.A.; Base, W.; Hoerl, W.H.; Wagner, L.Localization and characterization of the novel protein encoded by C20orf3Biochem. J.414485-4952008Homo sapiens PubMed
698520Kurita, O.Increase of acetate ester-hydrolyzing esterase activity in mixed cultures of Saccharomyces cerevisiae and Pichia anomalaJ. Appl. Microbiol.1041051-10582008Saccharomyces cerevisiae, Wickerhamomyces anomalus PubMed
699069Monti, D.; Ferrandi, E.E.; Righi, M.; Romano, D.; Molinari, F.Purification and characterization of the enantioselective esterase from Kluyveromyces marxianus CBS 1553J. Biotechnol.13365-722008Kluyveromyces marxianus, Kluyveromyces marxianus CBS 1553 PubMed
701087DAmbrosio, C.; Mandrich, L.; Rossi, M.; Scaloni, A.; Manco, G.A proteomic approach to study Escherichia coli. Acetyl esterase interactors unveil a sequence motif involved in protein-protein interactionProtein Pept. Lett.15333-3402008Escherichia coli PubMed
701417Chungool, W.; Thongkam, W.; Raweesri, P.; Thamchaipenet, A.; Pinphanichakarn, P.Production, purification, and characterization of acetyl esterase from Streptomyces sp. PC22 and its action in cooperation with xylanolytic enzymes on xylan degradationWorld J. Microbiol. Biotechnol.24549-5562008Streptomyces sp.-
715029Topakas, E.; Kyriakopoulos, S.; Biely, P.; Hirsch, J.; Vafiadi, C.; Christakopoulos, P.Carbohydrate esterases of family 2 are 6-O-deacetylasesFEBS Lett.584543-5482010Cellvibrio japonicus, Clostridium thermocellum PubMed
715373Pickard, D.; Toribio, A.L.; Petty, N.K.; van Tonder, A.; Yu, L.; Goulding, D.; Barrell, B.; Rance, R.; Harris, D.; Wetter, M.; Wain, J.; Choudhary, J.; Thomson, N.; Dougan, G.A conserved acetyl esterase domain targets diverse bacteriophages to the Vi capsular receptor of Salmonella enterica serovar TyphiJ. Bacteriol.1925746-57542010Salmonella enterica serovar Typhi Vi phage I, Salmonella enterica serovar Typhi Vi phage III, Salmonella enterica serovar Typhi Vi phage IV, Salmonella enterica serovar Typhi Vi phage V, Salmonella enterica serovar Typhi Vi phage VI, Salmonella enterica serovar Typhi Vi phage VII PubMed
715950Kobayashi, R.; Hirano, N.; Kanaya, S.; Saito, I.; Haruki, M.Enhancement of the enzymatic activity of Escherichia coli acetyl esterase by random mutagenesisJ. Mol. Catal. B67155-1612010Escherichia coli (P23872)-

LINKS TO OTHER DATABASES (specific for EC-Number 3.1.1.6)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)