Information on EC 3.1.1.59 - Juvenile-hormone esterase:
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The expected taxonomic range for this enzyme is: Endopterygota

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EC NUMBERCOMMENTARY
3.1.1.59-

RECOMMENDED NAMEGeneOntology No.
Juvenile-hormone esteraseGO:0004453

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
Methyl (2E,6E)-(10R,11S)-10,11-epoxy-3,7,11-trimethyltrideca-2,6-dienoate + H2O = (2E,6E)-(10R,11S)-10,11-epoxy-3,7,11-trimethyltrideca-2,6-dienoate + methanol
show the reaction diagram		----

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
hydrolysis of carboxylic ester----

PATHWAYKEGG LinkMetaCyc Link
Biosynthesis of secondary metabolites01110 -
Insect hormone biosynthesis00981 -

SYSTEMATIC NAMEIUBMB Comments
Methyl-(2E,6E)-(10R,11S)-10,11-epoxy-3,7,11-trimethyltrideca-2,6-dienoate acylhydrolaseDemethylates the insect juvenile hormones, JH1 and JH3, but does not hydrolyse the analogous ethyl or isopropyl esters.

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
Amjhe-likeApis melliferaQ76LA5contains main functional motifs of insect JHEs697457
BmCCE-5Bombyx moriD2KWC2-715173
EAT43357Aedes aegyptiQ17AV1-680159
Esterase, juvenile hormone----
JH esterase----
JH esteraseTrichoplusia ni--669746
JH esteraseDrosophila melanogaster, Drosophila melanogaster AANAT2, Drosophila melanogaster Canton S, Drosophila melanogaster CuZn-SOD, Drosophila melanogaster MH19, Drosophila melanogaster Oregon K, Drosophila melanogaster OregonR, Drosophila melanogaster Oregon R, Drosophila melanogaster Oregon-R, Drosophila melanogaster S-2, Drosophila melanogaster Samarkand--698082
JH esteraseReticulitermes flavipesD8UUL2-713975
JH esteraseApis mellifera--715819
JH III esteraseHelicoverpa zea--681234
JH-esterase----
JH-specific esteraseBombyx moriD2KWC2; 715173
JH-specific esteraseBombyx mori 122, Bombyx mori Bm-N, Bombyx mori C108, Bombyx mori P21, Bombyx mori P22, Bombyx mori Shogetu-Shunrei--715173
JHEIps typographus--649497
JHELeptinotarsa decemlineata--649498
JHEGryllus assimilis--649644
JHETribolium freemani--651397
JHELymantria dispar--651502
JHERhyparobia maderae--651504
JHEHeliothis virescens, Trichoplusia ni--651510
JHETenebrio molitor--649497, 651510, 651512
JHEChoristoneura fumiferana--653222
JHEDrosophila melanogaster, Drosophila melanogaster AANAT2, Drosophila melanogaster Canton S, Drosophila melanogaster CuZn-SOD, Drosophila melanogaster MH19, Drosophila melanogaster Oregon K, Drosophila melanogaster OregonR, Drosophila melanogaster Oregon R, Drosophila melanogaster Oregon-R, Drosophila melanogaster S-2, Drosophila melanogaster Samarkand--651500, 698082
JHEManduca sexta--651510, 651511, 707525
JHEDysdercus cingulatus--708700
JHEBombyx mori--651397, 677841, 715173
JHEBombyx mori 122, Bombyx mori Bm-N, Bombyx mori C108, Bombyx mori P21, Bombyx mori P22, Bombyx mori Shogetu-Shunrei--651397, 715173
JHETribolium castaneumD7US45-715184
JHEApis mellifera--715819
Juvenile hormone analog esterase----
Juvenile hormone carboxyesterase----
Juvenile hormone esterase----
Juvenile hormone esteraseDrosophila melanogaster, Drosophila melanogaster AANAT2, Drosophila melanogaster Canton S, Drosophila melanogaster CuZn-SOD, Drosophila melanogaster MH19, Drosophila melanogaster Oregon K, Drosophila melanogaster OregonR, Drosophila melanogaster Oregon R, Drosophila melanogaster Oregon-R, Drosophila melanogaster S-2, Drosophila melanogaster Samarkand--698082
Juvenile hormone esteraseManduca sexta--707525
Juvenile hormone esteraseDysdercus cingulatus--708700
Juvenile hormone esteraseTribolium castaneumD7US45-715184
juvenile hormone esterase-likeApis melliferaQ76LA5-697457
juvenile hormone-selective esteraseBombyx moriD2KWC2-715173
juvenile hormone-selective esteraseBombyx mori 122, Bombyx mori Bm-N, Bombyx mori C108, Bombyx mori P21, Bombyx mori P22, Bombyx mori Shogetu-Shunrei--715173
MsJHEManduca sextaP19985-667723
PhJHEPsacothea hilarisQ1HA48-680155

CAS REGISTRY NUMBERCOMMENTARY
50812-15-2-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Aedes aegypti-680159Q17AV1SwissProtManually annotated by BRENDA team
Apis mellifera-715819--Manually annotated by BRENDA team
Apis melliferahoney bee, africanized697457Q76LA5SwissProtManually annotated by BRENDA team
Bombyx mori-23492--Manually annotated by BRENDA team
Bombyx mori-677841Q6RVD1UniProtManually annotated by BRENDA team
Bombyx morigene Bmcce-5715173D2KWC2UniProtManually annotated by BRENDA team
Bombyx morigene Bmjhe715173--Manually annotated by BRENDA team
Bombyx morihighest activity at day 6 of the embryonic stage670670--Manually annotated by BRENDA team
Bombyx morihybrid-strain Shogetu-Shunrei651397--Manually annotated by BRENDA team
Bombyx mori Shogetu-Shunreihybrid-strain Shogetu-Shunrei651397--Manually annotated by BRENDA team
Choristoneura fumiferanaspruce budworm653222--Manually annotated by BRENDA team
Drosophila melanogaster-651500--Manually annotated by BRENDA team
Drosophila melanogaster-680156A1ZA98UniProtManually annotated by BRENDA team
Drosophila melanogasterstrains EP835 (reduced expression of DmP29), EP840, and Oregon R (control)698082--Manually annotated by BRENDA team
Dysdercus cingulatus-680125, 708700--Manually annotated by BRENDA team
Galleria mellonella-23470, 23478, 23483--Manually annotated by BRENDA team
Gryllus assimilis-680154A5HSI6SwissProtManually annotated by BRENDA team
Gryllus assimilisthree pairs of high- and low-selected JHE activity lines, no multiple isozymes649644--Manually annotated by BRENDA team
Helicoverpa zea-23475, 23492, 681234--Manually annotated by BRENDA team
Heliothis virescens-23475, 23488, 23492P12992SwissProtManually annotated by BRENDA team
Heliothis virescens-651510--Manually annotated by BRENDA team
Heliothis virescensrecombinant enzyme secreted into the cell medium in a baculovirus expression system23473P12992SwissProtManually annotated by BRENDA team
Hyphantria cunea-23493--Manually annotated by BRENDA team
insecta-23470--Manually annotated by BRENDA team
Ips typographus-649497--Manually annotated by BRENDA team
Isia isabella-23493--Manually annotated by BRENDA team
Leptinotarsa decemlineata-649498--Manually annotated by BRENDA team
Lymantria dispar-23470, 23474, 651502--Manually annotated by BRENDA team
Lymantria dispar-681237Q7M4I4UniProtManually annotated by BRENDA team
Manduca sexta-23470, 23472, 23480, 23482, 23489, 23492, 23494, 651510, 651511--Manually annotated by BRENDA team
Manduca sexta-667723P19985UniProtManually annotated by BRENDA team
Manduca sexta-667723, 707525Q9GPG0SwissProtManually annotated by BRENDA team
Manduca sextatobacco hornworm23471--Manually annotated by BRENDA team
Neobellieria bullata-23470--Manually annotated by BRENDA team
Periplaneta brunnea-23470--Manually annotated by BRENDA team
Psacothea hilaris-680155Q1HA48SwissProtManually annotated by BRENDA team
Reticulitermes flavipesgene RfEst1713975D8UUL2UniProtManually annotated by BRENDA team
Rhyparobia maderae-651504--Manually annotated by BRENDA team
Samia cynthia-23470--Manually annotated by BRENDA team
Schistocerca gregaria-23485--Manually annotated by BRENDA team
Schistocerca nitens-23470--Manually annotated by BRENDA team
Spodoptera exigua-23493--Manually annotated by BRENDA team
Spodoptera frugiperda-23491--Manually annotated by BRENDA team
Tenebrio molitor-23483, 649497, 651510--Manually annotated by BRENDA team
Tenebrio molitor-651512Q8WRD8SwissProtManually annotated by BRENDA team
Tribolium castaneumgenes Tcjhe-a, Tcjhe-b, Tcjhe-c, Tcjhe-d, and Tcjhe-e; genes Tcjhe-a, Tcjhe-b, Tcjhe-c, Tcjhe-d, and Tcjhe-e, encoding isozymes that vary only in the 5'-UTR sequences715184D7US45UniProtManually annotated by BRENDA team
Tribolium freemani-651397--Manually annotated by BRENDA team
Trichoplusia ni-23470, 23472, 23476, 23477, 23479, 23481, 23484, 23486, 23487, 23490, 23492, 23493, 23495, 651510--Manually annotated by BRENDA team
Trichoplusia nihigher activity in adult virgin female than in adult virgin male, much lower activity in starved organism compared to a fed control669746--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
physiological functionBombyx mori 122-BmJHE, a juvenile hormone-selective esterase, JHE, is important for juvenile hormone titer regulation in the silkworm Bombyx mori715173
physiological functionBombyx moriD2KWC2Bmcce-5 is strongly expressed in Malpighian tubules and the gut, it might function in digestion or xenobiotic metabolism; BmJHE, a juvenile hormone-selective esterase, JHE, is important for juvenile hormone titer regulation in the silkworm Bombyx mori715173
physiological functionBombyx mori Bm-N, Bombyx mori C108, Bombyx mori P21, Bombyx mori P22, Bombyx mori Shogetu-Shunrei-BmJHE, a juvenile hormone-selective esterase, JHE, is important for juvenile hormone titer regulation in the silkworm Bombyx mori715173
physiological functionTribolium castaneumD7US45JHEs are required for the degradation of juvenile hormones in insects715184
physiological functionApis mellifera-JHE is the enzyme primarily performing juvenile hormone degradation in Apis mellifera, while the JH epoxide hydrolase, EC 3.3.2.9, plays a negligible role715819

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
1-naphthyl acetate + H2O1-naphthol + acetate
show the reaction diagram		Dysdercus cingulatus--708700--?
1-naphthyl acetate + H2O1-naphthol + acetate
show the reaction diagram		Trichoplusia ni-13% of the activity with juvenile hormone I23487---
1-Naphthyl methylcarbonate + H2O1-Naphthol + methylcarbonate
show the reaction diagram		Trichoplusia ni-6% of the activity with juvenile hormone I23487---
4-nitrophenyl acetate + H2O4-nitrophenol + acetate
show the reaction diagram		Trichoplusia ni-69% of the activity with juvenile hormone I, slightly more active than with juvenile hormone III23487---
4-Nitrophenyl methylcarbonate + H2O4-Nitrophenol + methylcarbonate
show the reaction diagram		Trichoplusia ni-5% of the activity with juvenile hormone I23487---
alpha-naphthyl acetate + H2Oalpha-naphthol + acetate
show the reaction diagram		Tenebrio molitor--649497-649497?
alpha-naphthyl acetate + H2Oalpha-naphthol + acetate
show the reaction diagram		Trichoplusia ni--651510-651510?
alpha-naphthyl acetate + H2Oalpha-naphthol + acetate
show the reaction diagram		Tenebrio molitorQ8WRD8-651512-651512?
alpha-naphthyl acetate + H2Oalpha-naphthol + acetate
show the reaction diagram		Manduca sexta--707525--?
alpha-naphthyl acetate + H2Oalpha-naphthol + acetate
show the reaction diagram		Manduca sexta-activity decreases with increasing the chain length of the alpha-naphthyl substrates from acetate to octanoate651510-651510?
alpha-naphthyl acetate + H2Oalpha-naphthol + acetate
show the reaction diagram		Tenebrio molitor, Heliothis virescens-lower activity than with alpha-naphthyl propionate651510-651510?
alpha-naphthyl acetate + H2Oalpha-naphthol + acetate
show the reaction diagram		Ips typographus-specific activities of several developmental stages649497-649497?
alpha-naphthyl acetate + H2O?
show the reaction diagram		Drosophila melanogaster-artificial substrate651500-651500?
alpha-naphthyl butyrate + H2Oalpha-naphthol + butyrate
show the reaction diagram		Manduca sexta-activity decreases with increasing the chain length of the alpha-naphthyl substrates from acetate to octanoate651510-651510?
alpha-naphthyl butyrate + H2Oalpha-naphthol + butyrate
show the reaction diagram		Tenebrio molitor, Heliothis virescens-activity decreases with increasing the chain length of the alpha-naphthyl substrates from propionate to octanoate651510-651510?
alpha-naphthyl hexanoate + H2Oalpha-naphthol + hexanoate
show the reaction diagram		Manduca sexta-activity decreases with increasing the chain length of the alpha-naphthyl substrates from acetate to octanoate651510-651510?
alpha-naphthyl hexanoate + H2Oalpha-naphthol + hexanoate
show the reaction diagram		Tenebrio molitor, Heliothis virescens-activity decreases with increasing the chain length of the alpha-naphthyl substrates from propionate to octanoate651510-651510?
alpha-naphthyl octanoate + H2Oalpha-naphthol + octanoate
show the reaction diagram		Manduca sexta-activity decreases with increasing the chain length of the alpha-naphthyl substrates from acetate to octanoate651510-651510?
alpha-naphthyl octanoate + H2Oalpha-naphthol + octanoate
show the reaction diagram		Tenebrio molitor, Heliothis virescens-activity decreases with increasing the chain length of the alpha-naphthyl substrates from propionate to octanoate651510-651510?
alpha-naphthyl propionate + H2Oalpha-naphthol + propionate
show the reaction diagram		Manduca sexta-activity decreases with increasing the chain length of the alpha-naphthyl substrates from acetate to octanoate651510-651510?
alpha-naphthyl propionate + H2Oalpha-naphthol + propionate
show the reaction diagram		Tenebrio molitor, Heliothis virescens-higher activity than with alpha-naphthyl acetate, activity decreases with increasing the chain length of the alpha-naphthyl substrates from propionate to octanoate651510-651510?
HEPTAT + H2O?
show the reaction diagram		Tenebrio molitorQ8WRD8synthetic juvenile hormone analog651512-651512?
Juvenile hormone + H2OJuvenile hormone acid + acid diol
show the reaction diagram		insecta, Manduca sexta--23470---
Juvenile hormone + H2OJuvenile hormone acid + acid diol
show the reaction diagram		Manduca sexta--23471-23471-
Juvenile hormone + H2OJuvenile hormone acid + acid diol
show the reaction diagram		Manduca sexta--23472-23472-
Juvenile hormone + H2OJuvenile hormone acid + acid diol
show the reaction diagram		Samia cynthia, Trichoplusia ni--23470---
Juvenile hormone + H2OJuvenile hormone acid + acid diol
show the reaction diagram		Trichoplusia ni--23472-23472-
Juvenile hormone + H2OJuvenile hormone acid + acid diol
show the reaction diagram		Trichoplusia ni--23484---
Juvenile hormone + H2OJuvenile hormone acid + acid diol
show the reaction diagram		Galleria mellonella--23470, 23483---
Juvenile hormone + H2OJuvenile hormone acid + acid diol
show the reaction diagram		Periplaneta brunnea, Schistocerca nitens, Neobellieria bullata, Lymantria dispar--23470---
Juvenile hormone + H2OJuvenile hormone acid + acid diol
show the reaction diagram		Trichoplusia ni-juvenile hormone II23476, 23487---
Juvenile hormone + H2OJuvenile hormone acid + acid diol
show the reaction diagram		Manduca sexta-juvenile hormone III23480---
Juvenile hormone + H2OJuvenile hormone acid + acid diol
show the reaction diagram		Trichoplusia ni-juvenile hormone III23477, 23479, 23481, 23487---
Juvenile hormone + H2OJuvenile hormone acid + acid diol
show the reaction diagram		Lymantria dispar-juvenile hormone III23474---
Juvenile hormone + H2OJuvenile hormone acid + acid diol
show the reaction diagram		Schistocerca gregaria-C16 juvenile hormone-23H23485---
Juvenile hormone + H2OJuvenile hormone acid + acid diol
show the reaction diagram		Trichoplusia ni-juvenile hormone I23477, 23479, 23481, 23487---
Juvenile hormone + H2OJuvenile hormone acid + acid diol
show the reaction diagram		Galleria mellonella-juvenile hormone I23478---
juvenile hormone + H2O?
show the reaction diagram		Lymantria dispar--651502-651502?
juvenile hormone + H2O?
show the reaction diagram		Rhyparobia maderae--651504-651504?
juvenile hormone + H2O?
show the reaction diagram		Choristoneura fumiferana-juvenile hormone esterase activity653222-653222?
juvenile hormone + H2O?
show the reaction diagram		Manduca sexta-selective enzyme, hydrolyzes the methyl ester of juvenile hormone651511-651511?
juvenile hormone + H2O?
show the reaction diagram		Gryllus assimilis-endocrine regulator, degrades and inactivates a key developmental and reproductive hormone, juvenile hormone649644-649644?
juvenile hormone + H2O?
show the reaction diagram		Leptinotarsa decemlineata-juvenile hormone degradation649498-649498?
juvenile hormone + H2O?
show the reaction diagram		Manduca sexta-plays an important role in the regulation of metamorphosis in caterpillars, JHE is implicated in additional roles in development and reproduction651511-651511?
juvenile hormone + H2O?
show the reaction diagram		Tenebrio molitorQ8WRD8selective enzyme, juvenile hormone metabolism, alters the activity of juvenile hormone, which regulates development and reproduction651512-651512?
juvenile hormone + H2Ojuvenile hormone acid + methanol
show the reaction diagram		Tenebrio molitor, Manduca sexta, Trichoplusia ni, Heliothis virescens--651510-651510?
juvenile hormone + H2Ojuvenile hormone acid + methanol
show the reaction diagram		Ips typographus-JHE catabolizes juvenile hormone649497-649497?
juvenile hormone + H2Ojuvenile hormone acid + methanol
show the reaction diagram		Bombyx mori-key enzyme in the regulation of juvenile hormone level670670--?
juvenile hormone + H2Ojuvenile hormone acid + methanol
show the reaction diagram		Trichoplusia ni-key enzyme in the regulation of juvenile hormone level669746--?
juvenile hormone + H2Ojuvenile hormone acid + methanol
show the reaction diagram		Manduca sextaP19985, Q9GPG0key enzyme in the regulation of juvenile hormone level, degradation of juvenile hormone is required for proper insect development667723--?
juvenile hormone + H2Ojuvenile hormone acid + methanol
show the reaction diagram		Bombyx mori Shogetu-Shunrei, Bombyx mori C108, Bombyx mori P22, Bombyx mori P21, Bombyx mori Bm-N, Bombyx mori 122-key enzyme in the regulation of juvenile hormone level670670--?
juvenile hormone I + H2Ojuvenile hormone I acid + methanol
show the reaction diagram		Tenebrio molitor, Manduca sexta--651510-651510?
juvenile hormone I + H2Ojuvenile hormone I acid + methanol
show the reaction diagram		Trichoplusia ni--23477, 23479, 23481---
juvenile hormone I + H2Ojuvenile hormone I acid + methanol
show the reaction diagram		Galleria mellonella--23478---
juvenile hormone I + H2Ojuvenile hormone I acid + methanol
show the reaction diagram		Heliothis virescens--651510-651510?
juvenile hormone I + H2Ojuvenile hormone I acid + methanol
show the reaction diagram		Drosophila melanogasterA1ZA98-680156--?
juvenile hormone II + H2Ojuvenile hormone II acid + methanol
show the reaction diagram		Manduca sexta--707525--?
juvenile hormone III + H2O?
show the reaction diagram		Bombyx moriD2KWC2-715173--?
juvenile hormone III + H2O?
show the reaction diagram		Tribolium castaneumD7US45degradation715184--?
juvenile hormone III + H2O?
show the reaction diagram		Rhyparobia maderae-high affinity for juvenile hormone III651504-651504?
juvenile hormone III + H2O?
show the reaction diagram		Tenebrio molitorQ8WRD8hydrolyzes the methyl ester of juvenile hormone, highest specificity for juvenile hormone III651512-651512?
juvenile hormone III + H2O?
show the reaction diagram		Drosophila melanogaster-JHE is highly selective for JHIII and JHIII bisepoxide651500-651500?
juvenile hormone III + H2O?
show the reaction diagram		Leptinotarsa decemlineata-racemic649498-649498?
juvenile hormone III + H2O?
show the reaction diagram		Drosophila melanogaster-JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates651500-651500?
juvenile hormone III + H2O?
show the reaction diagram		Bombyx moriD2KWC2BmJHE is a juvenile hormone-selective esterase. Of the CCEs containing a GQSAG motif only BmJHE can function as a JH-specific degradation enzyme in the silkworm715173--?
juvenile hormone III + H2O?
show the reaction diagram		Bombyx moriD2KWC2BmCCE-5 has the ability to degrade juvenile hormone, however, the protein might not function as a JH-specific esterase in vivo as it has a high Km value for juvenile hormone715173--?
juvenile hormone III + H2O?
show the reaction diagram		Drosophila melanogaster MH19-JHE is highly selective for JHIII and JHIII bisepoxide, JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates651500-651500?
juvenile hormone III + H2O?
show the reaction diagram		Drosophila melanogaster CuZn-SOD-JHE is highly selective for JHIII and JHIII bisepoxide, JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates651500-651500?
juvenile hormone III + H2O?
show the reaction diagram		Drosophila melanogaster Oregon K-JHE is highly selective for JHIII and JHIII bisepoxide, JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates651500-651500?
juvenile hormone III + H2O?
show the reaction diagram		Drosophila melanogaster Oregon R-JHE is highly selective for JHIII and JHIII bisepoxide, JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates651500-651500?
juvenile hormone III + H2O?
show the reaction diagram		Bombyx mori Shogetu-Shunrei-BmJHE is a juvenile hormone-selective esterase. Of the CCEs containing a GQSAG motif only BmJHE can function as a JH-specific degradation enzyme in the silkworm715173--?
juvenile hormone III + H2O?
show the reaction diagram		Bombyx mori C108-BmJHE is a juvenile hormone-selective esterase. Of the CCEs containing a GQSAG motif only BmJHE can function as a JH-specific degradation enzyme in the silkworm715173--?
juvenile hormone III + H2O?
show the reaction diagram		Drosophila melanogaster Canton S-JHE is highly selective for JHIII and JHIII bisepoxide, JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates651500-651500?
juvenile hormone III + H2O?
show the reaction diagram		Drosophila melanogaster OregonR-JHE is highly selective for JHIII and JHIII bisepoxide, JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates651500-651500?
juvenile hormone III + H2O?
show the reaction diagram		Bombyx mori P22-BmJHE is a juvenile hormone-selective esterase. Of the CCEs containing a GQSAG motif only BmJHE can function as a JH-specific degradation enzyme in the silkworm715173--?
juvenile hormone III + H2O?
show the reaction diagram		Bombyx mori P21-BmJHE is a juvenile hormone-selective esterase. Of the CCEs containing a GQSAG motif only BmJHE can function as a JH-specific degradation enzyme in the silkworm715173--?
juvenile hormone III + H2O?
show the reaction diagram		Bombyx mori Bm-N-BmJHE is a juvenile hormone-selective esterase. Of the CCEs containing a GQSAG motif only BmJHE can function as a JH-specific degradation enzyme in the silkworm715173--?
juvenile hormone III + H2O?
show the reaction diagram		Drosophila melanogaster S-2-JHE is highly selective for JHIII and JHIII bisepoxide, JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates651500-651500?
juvenile hormone III + H2O?
show the reaction diagram		Drosophila melanogaster AANAT2-JHE is highly selective for JHIII and JHIII bisepoxide, JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates651500-651500?
juvenile hormone III + H2O?
show the reaction diagram		Drosophila melanogaster Samarkand-JHE is highly selective for JHIII and JHIII bisepoxide, JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates651500-651500?
juvenile hormone III + H2O?
show the reaction diagram		Drosophila melanogaster Oregon-R-JHE is highly selective for JHIII and JHIII bisepoxide, JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates651500-651500?
juvenile hormone III + H2O?
show the reaction diagram		Bombyx mori 122-BmJHE is a juvenile hormone-selective esterase. Of the CCEs containing a GQSAG motif only BmJHE can function as a JH-specific degradation enzyme in the silkworm715173--?
juvenile hormone III + H2Ojuvenile hormone III acid + methanol
show the reaction diagram		Bombyx mori--651397-651397?
juvenile hormone III + H2Ojuvenile hormone III acid + methanol
show the reaction diagram		Tenebrio molitorQ8WRD8-651512---
juvenile hormone III + H2Ojuvenile hormone III acid + methanol
show the reaction diagram		Tenebrio molitor, Manduca sexta--651510-651510?
juvenile hormone III + H2Ojuvenile hormone III acid + methanol
show the reaction diagram		Trichoplusia ni--23477, 23479, 23481---
juvenile hormone III + H2Ojuvenile hormone III acid + methanol
show the reaction diagram		Trichoplusia ni, Heliothis virescens--651510-651510?
juvenile hormone III + H2Ojuvenile hormone III acid + methanol
show the reaction diagram		Tribolium freemani--651397-651397?
juvenile hormone III + H2Ojuvenile hormone III acid + methanol
show the reaction diagram		Tenebrio molitor-highest specific activity649497-649497?
juvenile hormone III + H2Ojuvenile hormone III acid + methanol
show the reaction diagram		Gryllus assimilis-racemic649644juvenile hormone acid is the sole product649644?
juvenile hormone III + H2Ojuvenile hormone III acid + methanol
show the reaction diagram		Ips typographus-specific activities of several developmental stages, highest specific activity649497-649497?
juvenile hormone III + H2Ojuvenile hormone III acid + methanol
show the reaction diagram		Drosophila melanogasterA1ZA9810R-juvenile hormone III is the natural isomer680156--?
juvenile hormone III + H2Ojuvenile hormone III acid + methanol
show the reaction diagram		Manduca sexta-juvenile hormone III fits very tightly within the substrate binding pocket of JHE. This tight fit places two noncatalytic amino acid residues, Phe-259 and Thr-314, within the appropriate distance and geometry to potentially interact with the R,beta-unsaturated ester and epoxide, respectively, of juvenile hormone III. Neither nucleophilic attack of the resonance-stabilized ester by the catalytic serine nor the availability of a water molecule for attack of the acyl-enzyme intermediate appears to be a rate-determining step in the hydrolysis. Release of the juvenile hormone III acid metabolite from the substrate binding pocket limits the catalytic cycle. Chemical bond strength does not necessarily correlate with how reactive the bond will be to metabolism707525--?
juvenile hormone III + H2Ojuvenile hormone III acid + methanol
show the reaction diagram		Bombyx mori Shogetu-Shunrei, Bombyx mori C108, Bombyx mori P22, Bombyx mori P21, Bombyx mori Bm-N, Bombyx mori 122--651397-651397?
juvenile hormone III + H2Ojuvenile hormone acid + methanol
show the reaction diagram		Apis mellifera-JH esterase hydrolyzes the methyl ester to produce juvenile hormone acid715819--?
juvenile hormone III bisepoxide + H2O?
show the reaction diagram		Drosophila melanogaster-JHE is highly selective for JHIII and JHIII bisepoxide, JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates651500-651500?
juvenile hormone III bisepoxide + H2O?
show the reaction diagram		Drosophila melanogaster MH19-JHE is highly selective for JHIII and JHIII bisepoxide, JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates651500-651500?
juvenile hormone III bisepoxide + H2O?
show the reaction diagram		Drosophila melanogaster CuZn-SOD-JHE is highly selective for JHIII and JHIII bisepoxide, JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates651500-651500?
juvenile hormone III bisepoxide + H2O?
show the reaction diagram		Drosophila melanogaster Oregon K-JHE is highly selective for JHIII and JHIII bisepoxide, JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates651500-651500?
juvenile hormone III bisepoxide + H2O?
show the reaction diagram		Drosophila melanogaster Oregon R-JHE is highly selective for JHIII and JHIII bisepoxide, JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates651500-651500?
juvenile hormone III bisepoxide + H2O?
show the reaction diagram		Drosophila melanogaster Canton S-JHE is highly selective for JHIII and JHIII bisepoxide, JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates651500-651500?
juvenile hormone III bisepoxide + H2O?
show the reaction diagram		Drosophila melanogaster OregonR-JHE is highly selective for JHIII and JHIII bisepoxide, JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates651500-651500?
juvenile hormone III bisepoxide + H2O?
show the reaction diagram		Drosophila melanogaster S-2-JHE is highly selective for JHIII and JHIII bisepoxide, JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates651500-651500?
juvenile hormone III bisepoxide + H2O?
show the reaction diagram		Drosophila melanogaster AANAT2-JHE is highly selective for JHIII and JHIII bisepoxide, JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates651500-651500?
juvenile hormone III bisepoxide + H2O?
show the reaction diagram		Drosophila melanogaster Samarkand-JHE is highly selective for JHIII and JHIII bisepoxide, JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates651500-651500?
juvenile hormone III bisepoxide + H2O?
show the reaction diagram		Drosophila melanogaster Oregon-R-JHE is highly selective for JHIII and JHIII bisepoxide, JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates651500-651500?
juvenile hormone III diol + H2O?
show the reaction diagram		Manduca sexta-although hydrogen bonding appears not to occur between juvenile hormone III diol and Thr-314, the hydroxyls of the diol (at C10 and C11) are within hydrogen bonding distance of the main chain carbonyl oxygen atoms of Phe-259 and Phe-311707525--?
methyl (2E,6E)-(10R,11S)-10,11-epoxy-3,7,11-trimethyltrideca-2,6-dienoate + H2O(2E,6E)-(10R,11S)-10,11-epoxy-3,7,11-trimethyltrideca-2,6-dienoate + methanol
show the reaction diagram		Aedes aegyptiQ17AV1juvenile hormone esterase plays an important role in regulating juvenile hormone titers680159--?
methyl farnesoate + H2Omethanol + farnesoic acid
show the reaction diagram		Drosophila melanogasterA1ZA98-680156--?
methyl hepthylthioacetothioate + H2O?
show the reaction diagram		Bombyx moriD2KWC2a synthetic substrate715173--?
methyl hepthylthioacetothioate + H2O?
show the reaction diagram		Tribolium castaneumD7US45the recombinant JHE has a strong ability to degrade methyl hepthylthioacetothioate, HEPTAT, as a synthetic substrate715184--?
methyl hexylthio acetothioate + H2O?
show the reaction diagram		Tenebrio molitor-putative surrogate substrate for juvenile hormone649497-649497?
p-nitrophenyl acetate + H2Op-nitrophenol + acetate
show the reaction diagram		Trichoplusia ni--651510-651510?
p-nitrophenyl acetate + H2Op-nitrophenol + acetate
show the reaction diagram		Tenebrio molitorQ8WRD8-651512-651512?
p-nitrophenyl acetate + H2Op-nitrophenol + acetate
show the reaction diagram		Tenebrio molitor, Manduca sexta, Heliothis virescens-higher specific activity than for p-nitrophenyl valerate651510-651510?
p-nitrophenyl valerate + H2Op-nitrophenol + valerate
show the reaction diagram		Tenebrio molitor, Manduca sexta, Heliothis virescens-lower specific activity than for p-nitrophenyl acetate651510-651510?
saturated juvenile hormone III + H2Osaturated juvenile hormone III acid + methanol
show the reaction diagram		Manduca sexta-alpha,beta-saturated methyl ester707525--?
methyl hexylthio acetothioate + H2O?
show the reaction diagram		Ips typographus-putative surrogate substrate for juvenile hormone, specific activities of several developmental stages649497-649497?
additional information?-Trichoplusia ni-low activity with hydroprene and methoprene23479---
additional information?-Spodoptera frugiperda-effectiveness of thioester compounds as surrogate substrates23491---
additional information?-insecta, Manduca sexta, Samia cynthia, Trichoplusia ni, Galleria mellonella, Periplaneta brunnea, Schistocerca nitens, Neobellieria bullata, Lymantria dispar-demethylates the insect juvenile hormones, JH1 and JH3, but does not hydrolyze the analogous ethyl or isopropyl esters23470---
additional information?-Drosophila melanogaster-structural features promoting substrate binding at the active site of JHE651500-651500?
additional information?-Bombyx mori-likely to be involved in elimination of intracellular juvenile hormone from the target tissues in the preparation for metamorphosis23492---
additional information?-Manduca sexta-likely to be involved in elimination of intracellular juvenile hormone from the target tissues in the preparation for metamorphosis23471, 23492---
additional information?-Trichoplusia ni, Heliothis virescens, Helicoverpa zea-likely to be involved in elimination of intracellular juvenile hormone from the target tissues in the preparation for metamorphosis23492---
additional information?-insecta, Manduca sexta, Samia cynthia, Trichoplusia ni, Galleria mellonella, Periplaneta brunnea, Schistocerca nitens, Neobellieria bullata, Lymantria dispar-demethylates the insect juvenile hormones, JH1 and JH3, major role in regulation of juvenile hormone titer and may be necessary for the removal of all traces of juvenile hormone at periods critical to further development23470---
additional information?-Choristoneura fumiferana-hormonal regulation of enzyme653222-653222?
additional information?-Dysdercus cingulatus-the enzyme plays a profound role in metamorphosis and reproduction680125---
additional information?-Drosophila melanogasterA1ZA98there is probably only one physiologically functional JHE in Drosophila melanogaster but multiple esterases with JH esterase activity, no activity with juvenile hormone II680156---
additional information?-Apis melliferaQ76LA5clearance of juvenile hormone during larval-pupal metamorphosis697457---
additional information?-Apis melliferaQ76LA5deficiency in Amjhe-like results in 6fold increase in juvenile hormone titer (adult, 4 day-old worker bee)697457---
additional information?-Reticulitermes flavipesD8UUL2the enzyme is also active on alpha-naphthyl propionate, alpha-naphthyl butyrate, and 2-carbon substrate alpha-naphthyl acetate713975---
additional information?-Bombyx moriD2KWC2the enzymes encoded by genes Bmcce-1-4 are not active on juvenile hormone715173---
additional information?-Drosophila melanogaster MH19, Drosophila melanogaster CuZn-SOD-structural features promoting substrate binding at the active site of JHE651500-651500?
additional information?-Spodoptera frugiperda Sf9-effectiveness of thioester compounds as surrogate substrates23491---
additional information?-Drosophila melanogaster Oregon K, Drosophila melanogaster Oregon R-structural features promoting substrate binding at the active site of JHE651500-651500?
additional information?-Bombyx mori Shogetu-Shunrei-likely to be involved in elimination of intracellular juvenile hormone from the target tissues in the preparation for metamorphosis23492---
additional information?-Spodoptera frugiperda Sf-21-effectiveness of thioester compounds as surrogate substrates23491---
additional information?-Bombyx mori C108-likely to be involved in elimination of intracellular juvenile hormone from the target tissues in the preparation for metamorphosis23492---
additional information?-Drosophila melanogaster Canton S, Drosophila melanogaster OregonR-structural features promoting substrate binding at the active site of JHE651500-651500?
additional information?-Bombyx mori P22, Bombyx mori P21, Bombyx mori Bm-N-likely to be involved in elimination of intracellular juvenile hormone from the target tissues in the preparation for metamorphosis23492---
additional information?-Drosophila melanogaster S-2, Drosophila melanogaster AANAT2, Drosophila melanogaster Samarkand, Drosophila melanogaster Oregon-R-structural features promoting substrate binding at the active site of JHE651500-651500?
additional information?-Bombyx mori 122-likely to be involved in elimination of intracellular juvenile hormone from the target tissues in the preparation for metamorphosis23492---

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
juvenile hormone + H2O?
show the reaction diagram		Gryllus assimilis-endocrine regulator, degrades and inactivates a key developmental and reproductive hormone, juvenile hormone649644-649644
juvenile hormone + H2O?
show the reaction diagram		Leptinotarsa decemlineata-juvenile hormone degradation649498-649498
juvenile hormone + H2O?
show the reaction diagram		Manduca sexta-plays an important role in the regulation of metamorphosis in caterpillars, JHE is implicated in additional roles in development and reproduction651511-651511
juvenile hormone + H2O?
show the reaction diagram		Tenebrio molitorQ8WRD8selective enzyme, juvenile hormone metabolism, alters the activity of juvenile hormone, which regulates development and reproduction651512-651512
juvenile hormone + H2Ojuvenile hormone acid + methanol
show the reaction diagram		Tenebrio molitor, Manduca sexta, Trichoplusia ni, Heliothis virescens--651510-651510
juvenile hormone + H2Ojuvenile hormone acid + methanol
show the reaction diagram		Ips typographus-JHE catabolizes juvenile hormone649497-649497
juvenile hormone + H2Ojuvenile hormone acid + methanol
show the reaction diagram		Bombyx mori-key enzyme in the regulation of juvenile hormone level670670--
juvenile hormone + H2Ojuvenile hormone acid + methanol
show the reaction diagram		Trichoplusia ni-key enzyme in the regulation of juvenile hormone level669746--
juvenile hormone + H2Ojuvenile hormone acid + methanol
show the reaction diagram		Manduca sextaP19985, Q9GPG0key enzyme in the regulation of juvenile hormone level, degradation of juvenile hormone is required for proper insect development667723--
juvenile hormone + H2Ojuvenile hormone acid + methanol
show the reaction diagram		Bombyx mori Shogetu-Shunrei, Bombyx mori C108, Bombyx mori P22, Bombyx mori P21, Bombyx mori Bm-N, Bombyx mori 122-key enzyme in the regulation of juvenile hormone level670670--
juvenile hormone III + H2O?
show the reaction diagram		Tribolium castaneumD7US45degradation715184--
juvenile hormone III + H2O?
show the reaction diagram		Drosophila melanogaster-JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates651500-651500
juvenile hormone III + H2O?
show the reaction diagram		Bombyx moriD2KWC2BmJHE is a juvenile hormone-selective esterase. Of the CCEs containing a GQSAG motif only BmJHE can function as a JH-specific degradation enzyme in the silkworm715173--
juvenile hormone III + H2Ojuvenile hormone acid + methanol
show the reaction diagram		Apis mellifera-JH esterase hydrolyzes the methyl ester to produce juvenile hormone acid715819--
juvenile hormone III + H2O?
show the reaction diagram		Drosophila melanogaster MH19, Drosophila melanogaster CuZn-SOD, Drosophila melanogaster Oregon K, Drosophila melanogaster Oregon R-JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates651500-651500
juvenile hormone III + H2O?
show the reaction diagram		Bombyx mori Shogetu-Shunrei, Bombyx mori C108-BmJHE is a juvenile hormone-selective esterase. Of the CCEs containing a GQSAG motif only BmJHE can function as a JH-specific degradation enzyme in the silkworm715173--
juvenile hormone III + H2O?
show the reaction diagram		Drosophila melanogaster Canton S, Drosophila melanogaster OregonR-JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates651500-651500
juvenile hormone III + H2O?
show the reaction diagram		Bombyx mori P22, Bombyx mori P21, Bombyx mori Bm-N-BmJHE is a juvenile hormone-selective esterase. Of the CCEs containing a GQSAG motif only BmJHE can function as a JH-specific degradation enzyme in the silkworm715173--
juvenile hormone III + H2O?
show the reaction diagram		Drosophila melanogaster S-2, Drosophila melanogaster AANAT2, Drosophila melanogaster Samarkand, Drosophila melanogaster Oregon-R-JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates651500-651500
juvenile hormone III + H2O?
show the reaction diagram		Bombyx mori 122-BmJHE is a juvenile hormone-selective esterase. Of the CCEs containing a GQSAG motif only BmJHE can function as a JH-specific degradation enzyme in the silkworm715173--
juvenile hormone III bisepoxide + H2O?
show the reaction diagram		Drosophila melanogaster, Drosophila melanogaster MH19, Drosophila melanogaster CuZn-SOD, Drosophila melanogaster Oregon K, Drosophila melanogaster Oregon R, Drosophila melanogaster Canton S, Drosophila melanogaster OregonR, Drosophila melanogaster S-2, Drosophila melanogaster AANAT2, Drosophila melanogaster Samarkand, Drosophila melanogaster Oregon-R-JHE is highly selective for JHIII and JHIII bisepoxide, both may be in vivo substrates651500-651500
methyl (2E,6E)-(10R,11S)-10,11-epoxy-3,7,11-trimethyltrideca-2,6-dienoate + H2O(2E,6E)-(10R,11S)-10,11-epoxy-3,7,11-trimethyltrideca-2,6-dienoate + methanol
show the reaction diagram		Aedes aegyptiQ17AV1juvenile hormone esterase plays an important role in regulating juvenile hormone titers680159--
additional information?-Bombyx mori-likely to be involved in elimination of intracellular juvenile hormone from the target tissues in the preparation for metamorphosis23492--
additional information?-Manduca sexta-likely to be involved in elimination of intracellular juvenile hormone from the target tissues in the preparation for metamorphosis23471, 23492--
additional information?-Trichoplusia ni, Heliothis virescens, Helicoverpa zea-likely to be involved in elimination of intracellular juvenile hormone from the target tissues in the preparation for metamorphosis23492--
additional information?-insecta, Manduca sexta, Samia cynthia, Trichoplusia ni, Galleria mellonella, Periplaneta brunnea, Schistocerca nitens, Neobellieria bullata, Lymantria dispar-demethylates the insect juvenile hormones, JH1 and JH3, major role in regulation of juvenile hormone titer and may be necessary for the removal of all traces of juvenile hormone at periods critical to further development23470--
additional information?-Choristoneura fumiferana-hormonal regulation of enzyme653222-653222
additional information?-Dysdercus cingulatus-the enzyme plays a profound role in metamorphosis and reproduction680125--
additional information?-Drosophila melanogasterA1ZA98there is probably only one physiologically functional JHE in Drosophila melanogaster but multiple esterases with JH esterase activity680156--
additional information?-Apis melliferaQ76LA5clearance of juvenile hormone during larval-pupal metamorphosis697457--
additional information?-Apis melliferaQ76LA5deficiency in Amjhe-like results in 6fold increase in juvenile hormone titer (adult, 4 day-old worker bee)697457--
additional information?-Bombyx mori Shogetu-Shunrei, Bombyx mori C108, Bombyx mori P22, Bombyx mori P21, Bombyx mori Bm-N, Bombyx mori 122-likely to be involved in elimination of intracellular juvenile hormone from the target tissues in the preparation for metamorphosis23492--

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
No entries in this field

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
No entries in this field

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
1,1,1-trifluoro-3-(octane-1-sulfonyl)propan-2,2-diolTenebrio molitorQ8WRD8IC50: 0.0016 mM651512 2D-image
1,1,1-trifluoro-3-(octylsulfanyl)propan-2-oneDysdercus cingulatus--680125 2D-image
1,1,1-trifluoro-3-decylsulfanyl-propan-2-oneHeliothis virescens-IC50: 0.99 nM, juvenile hormone III hydrolysis651510 2D-image
1,1,1-trifluoro-3-decylsulfanyl-propan-2-oneManduca sexta-IC50: 2.8 nM, juvenile hormone III hydrolysis651510 2D-image
1,1,1-trifluoro-3-decylsulfanyl-propan-2-oneTenebrio molitor-IC50: 3.6 nM, juvenile hormone III hydrolysis651510 2D-image
1,1,1-trifluoro-3-decylsulfanyl-propan-2-oneTenebrio molitorQ8WRD8potent inhibitor, IC50: 3.6 nm651512 2D-image
1,1,1-trifluoro-3-dodecylsulfanyl-propan-2-oneHeliothis virescens-IC50: 2.2 nM, juvenile hormone III hydrolysis651510 2D-image
1,1,1-trifluoro-3-dodecylsulfanyl-propan-2-oneManduca sexta-IC50: 4.4 nM, juvenile hormone III hydrolysis651510 2D-image
1,1,1-trifluoro-3-dodecylsulfanyl-propan-2-oneTenebrio molitor-IC50: 12 nM, juvenile hormone III hydrolysis651510 2D-image
1,1,1-trifluoro-3-hexylsulfanyl-propan-2-oneHeliothis virescens-IC50: 1100 nM, juvenile hormone III hydrolysis651510 2D-image
1,1,1-trifluoro-3-hexylsulfanyl-propan-2-oneManduca sexta-IC50: 1700 nM, juvenile hormone III hydrolysis651510 2D-image
1,1,1-trifluoro-3-hexylsulfanyl-propan-2-oneTenebrio molitor-IC50: 14 nM, juvenile hormone III hydrolysis651510 2D-image
1,1,1-trifluoro-3-octylsulfanyl-propan-2-oneHeliothis virescens-IC50: 5.8 nM, juvenile hormone III hydrolysis651510 2D-image
1,1,1-trifluoro-3-octylsulfanyl-propan-2-oneManduca sexta-IC50: 9.1 nM, juvenile hormone III hydrolysis651510 2D-image
1,1,1-trifluoro-3-octylsulfanyl-propan-2-oneTenebrio molitor-IC50: 4.6 nM, juvenile hormone III hydrolysis651510 2D-image
1,1,1-trifluoro-3-octylsulfanyl-propan-2-oneTenebrio molitorQ8WRD8potent inhibitor, IC50: 4.6 nm651512 2D-image
1,1,1-trifluoro-3-phenethylsulfanyl-propan-2-oneHeliothis virescens-IC50: 75 nM, juvenile hormone III hydrolysis651510 2D-image
1,1,1-trifluoro-3-phenethylsulfanyl-propan-2-oneManduca sexta-IC50: 400 nM, juvenile hormone III hydrolysis651510 2D-image
1,1,1-trifluoro-3-phenethylsulfanyl-propan-2-oneTenebrio molitor-IC50: 36 nM, juvenile hormone III hydrolysis651510 2D-image
1-octyl (1-(3,3,3-trifluoropropan-2,2-dihydroxy))sulfoneTrichoplusia ni-potent, specific inhibitor651510 2D-image
3-(4'-Butylphenylthio)-1,1,1-trifluoropropan-2-oneManduca sexta--23494 2D-image
3-octylthio-1,1,1-trifluoro-2-propanoneIps typographus-more inhibitory than 3-pentylthio-1,1,1-trifluoro-2-propanone and diisopropyl fluorophosphate, moderately potent inhibitor649497 2D-image
3-octylthio-1,1,1-trifluoro-2-propanoneTenebrio molitor-very potent inhibitor649497 2D-image
3-octylthio-1,1,1-trifluoro-2-propanoneGryllus assimilis-potent inhibitor, pI50: 7.3-7.8, high- and low-selected JHE activity lines649644 2D-image
3-octylthio-1,1,1-trifluoro-2-propanoneApis mellifera-specific inhibitor, 95-100% inhbition at 2 mM in hemolymph and fat body715819 2D-image
3-Octylthio-1,1,1-trifluoropropan-2-oneTrichoplusia ni-high affinity and slow binding inhibitor23477 2D-image
3-Octylthio-1,1,1-trifluoropropan-2-oneManduca sexta--23489, 23494 2D-image
3-Octylthio-1,1,1-trifluoropropan-2-oneManduca sextaP19985transition state analogue inhibitor; transition state analogue inhibitor, IC50 (wild type): 22 nM, IC50 (F259I mutant): 330 nM667723 2D-image
3-pentylthio-1,1,1-trifluoro-2-propanoneIps typographus, Tenebrio molitor-less inhibitory than 3-octylthio-1,1,1-trifluoro-2-propanone, more inhibitory than diisopropyl fluorophosphate649497 2D-image
3-Substituted 1,1,1-trifluoropropanone sulfidesBombyx mori, Bombyx mori 122, Bombyx mori Bm-N, Bombyx mori C108, Bombyx mori P21, Bombyx mori P22, Bombyx mori Shogetu-Shunrei, Helicoverpa zea, Heliothis virescens, Manduca sexta--23492 2D-image
3-[(4'-Mercapto)butylthio]-1,1,1-trifluoropropan-2-oneManduca sexta--23489 2D-image
AlkylthiotrifluoropropanonesHelicoverpa zea, Heliothis virescens--23475 2D-image
bis-(4-nitrophenyl) phosphateSchistocerca gregaria--23485 2D-image
CarbarylTenebrio molitorQ8WRD8weak, IC50: above 0.1 mM651512 2D-image
ChlorpromazineBombyx mori, Bombyx mori 122, Bombyx mori Bm-N, Bombyx mori C108, Bombyx mori P21, Bombyx mori P22, Bombyx mori Shogetu-Shunrei-octopamine antagonist, reduces JHE activity, when applied in diet at 10-100 ppm651397 2D-image
DichlorvosSchistocerca gregaria--23485 2D-image
diisopropyl fluorophosphateIps typographus, Tenebrio molitor-less inhibitory than 3-octylthio-1,1,1-trifluoro-2-propanone and 3-pentylthio-1,1,1-trifluoro-2-propanone, poor inhibitor649497 2D-image
diisopropyl fluorophosphateDrosophila melanogasterA1ZA9850% inhibition at 0.002 mM680156 2D-image
eserineGryllus assimilis-IC50: 3.9-4.2, high- and low-selected JHE activity lines649644 2D-image
gramineBombyx mori, Bombyx mori 122, Bombyx mori Bm-N, Bombyx mori C108, Bombyx mori P21, Bombyx mori P22, Bombyx mori Shogetu-Shunrei-octopamine antagonist, reduces JHE activity, when applied in diet at 10-100 ppm651397 2D-image
hydropreneDrosophila melanogasterA1ZA9850% inhibition at 8 mM680156 2D-image
JHE binding proteinDrosophila melanogaster, Drosophila melanogaster AANAT2, Drosophila melanogaster Canton S, Drosophila melanogaster CuZn-SOD, Drosophila melanogaster MH19, Drosophila melanogaster Oregon K, Drosophila melanogaster OregonR, Drosophila melanogaster Oregon R, Drosophila melanogaster Oregon-R, Drosophila melanogaster S-2, Drosophila melanogaster Samarkand-DmP29, induces anti-juvenile hormone effects698082-
Juvenile hormoneTrichoplusia ni-juvenile hormone I, II or III23479 2D-image
kinopreneDrosophila melanogasterA1ZA9850% inhibition at 2 mM680156 2D-image
methamidophosTenebrio molitorQ8WRD8weak, IC50: above 1 mM651512 2D-image
O,O-Dialkyl-O-4-nitrophenyl phosphatesTrichoplusia ni-O,O-dialkyl-O-4-nitrophenyl analog provides the greatest inhibition23479 2D-image
O,O-diethyl-p-nitrophenyl phosphateIps typographus, Tenebrio molitor-poor inhibitor649497 2D-image
O,O-diisopropyl phosphofluoridateGryllus assimilis-pI50: 5-5.7, high- and low-selected JHE activity lines649644 2D-image
O-ethyl-S-phenyl-phosphoramidothiolateTenebrio molitorQ8WRD8IC50: 0.0084 mM651512 2D-image
O-Ethyl-S-phosphoramidothiolateTrichoplusia ni-irreversible23479 2D-image
O-Ethyl-S-phosphoramidothiolateGalleria mellonella-Galleria mellonella enzyme inhibited, Tenebrio molitor enzyme not23483 2D-image
O-Ethyl-S-phosphoramidothiolateTrichoplusia ni--23484 2D-image
O-Ethyl-S-phosphoramidothiolateManduca sexta--23494 2D-image
octylthiotrifluoropropanoneDrosophila melanogasterA1ZA9850% inhibition below 1 nM680156 2D-image
ParaoxonGalleria mellonella--23483 2D-image
ParaoxonSchistocerca gregaria--23485 2D-image
ParaoxonBombyx mori, Bombyx mori 122, Bombyx mori Bm-N, Bombyx mori C108, Bombyx mori P21, Bombyx mori P22, Bombyx mori Shogetu-Shunrei, Helicoverpa zea, Heliothis virescens, Manduca sexta-irreversible23492 2D-image
ParaoxonTrichoplusia ni--23484, 23492 2D-image
PhenylmethylsulfonylfluorideIps typographus-moderate inhibitor649497 2D-image
PhenylmethylsulfonylfluorideTenebrio molitor-weak inhibitor649497 2D-image
PhenylmethylsulfonylfluorideTenebrio molitorQ8WRD8mediocre inhibitor, IC50: 0.24 mM651512 2D-image
phosphoramidothiolateManduca sexta-JHE is highly sensitive to certain phosphoramidothiolates651511 2D-image
trifluoroketone inhibitorManduca sexta-JHE is highly sensitive651511-
TrifluoroketonesSpodoptera frugiperda, Spodoptera frugiperda Sf-21, Spodoptera frugiperda Sf9--23491 2D-image
trifluoromethylketoneTrichoplusia ni-with chain lengths of 8, 10 or 12 carbons, highly effective inhibitors651510 2D-image
Tris-butylphosphorotrithiolateSchistocerca gregaria--23485 2D-image
Triton X-100Galleria mellonella, insecta, Lymantria dispar, Manduca sexta, Neobellieria bullata, Periplaneta brunnea, Samia cynthia, Schistocerca nitens, Trichoplusia ni--23470 2D-image
Triton X-100Tenebrio molitor-inhibits at low concentrations651510 2D-image
ZR-448Trichoplusia ni-a methyl ester dienoate juvenoid, competitive23479 2D-image
methopreneDrosophila melanogasterA1ZA9850% inhibition at 2 mM680156 2D-image
additional informationinsecta, Lymantria dispar, Neobellieria bullata, Periplaneta brunnea, Samia cynthia, Schistocerca nitens-highly resistant to diisopropyl fluorophosphate23470-
additional informationTrichoplusia ni--23470-
additional informationManduca sexta-highly resistant to diisopropyl fluorophosphate23470, 23471-
additional informationLymantria dispar-highly resistant to diisopropyl fluorophosphate; phenylmethylsulfonyl fluoride23474-
additional informationGalleria mellonella-highly resistant to diisopropyl fluorophosphate23470, 23483-
additional informationTrichoplusia ni-highly resistant to diisopropyl fluorophosphate (very little effect)23484-
additional informationManduca sexta-highly resistant to diisopropyl fluorophosphate; iodoacetate23494-
additional informationIps typographus-not inhibited by 1% Triton X-100, IC50-values for several inhibitors649497-
additional informationTenebrio molitor-IC50-values for several inhibitors649497-
additional informationDrosophila melanogaster, Drosophila melanogaster AANAT2, Drosophila melanogaster Canton S, Drosophila melanogaster CuZn-SOD, Drosophila melanogaster MH19, Drosophila melanogaster Oregon K, Drosophila melanogaster OregonR, Drosophila melanogaster Oregon R, Drosophila melanogaster Oregon-R, Drosophila melanogaster S-2, Drosophila melanogaster Samarkand-lipophorin might modulate juvenile hormone hydrolysis by competition for binding of juvenile hormone651500-
additional informationTrichoplusia ni-insects treated with different substances, trans-stilbene oxide do not alter JH esterase levels when topically applied, but oral administration reduces activity to 70%, cholesterol 5alpha,6alpha-epoxide reduces the JH esterase activity to 60–80% of the control, 3-octylthio-1,1,1-trifluoropropan-2-one strongly reduces JH esterase activity669746-

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
1-(4-chloro-2-methylphenyl)imidazolidine-2-thioneTribolium freemani-octopamine agonist, stimulates the pupation and JHE activity of larvae reared under crowded conditions, when topically applied, 0.05 mg651397 2D-image
2-(4-chloro-2-methylphenylimino)oxazolidineTribolium freemani-octopamine agonist, stimulates the pupation and JHE activity of larvae reared under crowded conditions, when topically applied, 0.05 mg651397 2D-image
acetoneHeliothis virescens-at low concentrations, slight activation651510 2D-image
acetoneManduca sexta-activates even at higher concentrations651510 2D-image
acetoneTrichoplusia ni-at low concentrations, slight activation651510 2D-image
chlordimeformTribolium freemani-octopamine agonist, stimulates the pupation and JHE activity of larvae reared under crowded conditions, when topically applied, 0.01 mg651397 2D-image
DetergentManduca sexta-activates at lower concentrations651511-
ethanolManduca sexta-activates even at higher concentrations651510 2D-image
OctopamineBombyx mori, Bombyx mori 122, Bombyx mori Bm-N, Bombyx mori C108, Bombyx mori P21, Bombyx mori P22, Bombyx mori Shogetu-Shunrei-exogenous octopamine, 0.1 -10 mM, stimulates JHE activity of final instars at day 2651397 2D-image
OctopamineTribolium freemani-10 mM, activates JHE activity of newly ecdysed pupae651397 2D-image
organic solventManduca sexta-activates at lower concentrations651511-
Triton X-100Heliothis virescens-at low concentrations, slight activation651510 2D-image
Triton X-100Manduca sexta-activates at low concentrations651510 2D-image
Triton X-100Trichoplusia ni-at low concentrations, slight activation651510 2D-image
IsopropanolManduca sexta-activates even at higher concentrations651510 2D-image
additional informationChoristoneura fumiferana-enzyme is induced by juvenile hormone I653222-
additional informationTrichoplusia ni-insects treated with different substances, methoprene, increases JH esterase by 2.5fold, juvenile hormone III increases JH esterase by 2.2 fold669746-

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.12-Alpha-naphthyl acetateDrosophila melanogaster, Drosophila melanogaster AANAT2, Drosophila melanogaster Canton S, Drosophila melanogaster CuZn-SOD, Drosophila melanogaster MH19, Drosophila melanogaster Oregon K, Drosophila melanogaster OregonR, Drosophila melanogaster Oregon R, Drosophila melanogaster Oregon-R, Drosophila melanogaster S-2, Drosophila melanogaster Samarkand--651500 2D-image
0.41-Alpha-naphthyl acetateManduca sexta-wild-type, at 30°C in 50 mM sodium phosphate buffer, pH 7.4, containing 1 mg/mL of bovine serum albumin707525 2D-image
0.0191-HEPTATTenebrio molitorQ8WRD8recombinant JHE651512 2D-image
0.00015-Juvenile hormoneRhyparobia maderae-about651504 2D-image
0.00038-Juvenile hormoneTrichoplusia ni-purified enzyme23476 2D-image
0.00179-Juvenile hormoneGalleria mellonella-Galleria mellonella23483 2D-image
0.00021-juvenile hormone IGalleria mellonella--23478 2D-image
0.0004-juvenile hormone ITrichoplusia ni--23479 2D-image
0.00113-juvenile hormone ITrichoplusia ni--23481 2D-image
4.2-juvenile hormone IDrosophila melanogasterA1ZA98-680156 2D-image
0.00034-juvenile hormone IITrichoplusia ni-second value 0.0025 mM, enzyme in crude hemolymph23476 2D-image
0.0025-juvenile hormone IITrichoplusia ni-second value 0.00034 mM, enzyme in crude hemolymph23476 2D-image
0.00003-juvenile hormone IIIManduca sexta-wild-type, at 30°C in 50 mM sodium phosphate buffer, pH 7.4, containing 1 mg/mL of bovine serum albumin707525 2D-image
0.000086-juvenile hormone IIIManduca sexta-mutant T314V, at 30°C in 50 mM sodium phosphate buffer, pH 7.4, containing 1 mg/mL of bovine serum albumin707525 2D-image
0.000089-juvenile hormone IIIDrosophila melanogaster, Drosophila melanogaster AANAT2, Drosophila melanogaster Canton S, Drosophila melanogaster CuZn-SOD, Drosophila melanogaster MH19, Drosophila melanogaster Oregon K, Drosophila melanogaster OregonR, Drosophila melanogaster Oregon R, Drosophila melanogaster Oregon-R, Drosophila melanogaster S-2, Drosophila melanogaster Samarkand--651500 2D-image
0.000101-juvenile hormone IIIManduca sexta-mutant F259I, at 30°C in 50 mM sodium phosphate buffer, pH 7.4, containing 1 mg/mL of bovine serum albumin707525 2D-image
0.00025-juvenile hormone IIITrichoplusia ni--23479 2D-image
0.000315-juvenile hormone IIITenebrio molitorQ8WRD8recombinant JHE651512 2D-image
0.00036-juvenile hormone IIILymantria dispar-racemic, (10R,11S)-enantiomer of juvenile hormone II23474 2D-image
0.000402-juvenile hormone IIITrichoplusia ni--23481 2D-image
0.000657-juvenile hormone IIITribolium castaneumD7US45pH and temperature not specified in the publication715184 2D-image
0.0014-juvenile hormone IIILeptinotarsa decemlineata-pH 8, major enzyme form with pI 5.6649498 2D-image
0.0022-juvenile hormone IIILeptinotarsa decemlineata-pH 8, minor enzyme form with pI 5.5649498 2D-image
0.0025-juvenile hormone IIIBombyx moriD2KWC2pH 7.4, 22°C, recombinant BmCCE-5715173 2D-image
0.0028-juvenile hormone IIIBombyx moriD2KWC2pH 7.4, 22°C, recombinant BmJHE715173 2D-image
0.0028-juvenile hormone IIIBombyx mori 122, Bombyx mori Bm-N, Bombyx mori C108, Bombyx mori P21, Bombyx mori P22, Bombyx mori Shogetu-Shunrei-pH 7.4, 22°C, recombinant BmJHE715173 2D-image
1.3-juvenile hormone IIIDrosophila melanogasterA1ZA9810R isomer of juvenile hormone III680156 2D-image
1.5-juvenile hormone IIIDrosophila melanogasterA1ZA98racemic juvenile hormone III680156 2D-image
2.4-juvenile hormone IIIDrosophila melanogasterA1ZA9810S isomer of juvenile hormone III680156 2D-image
0.000046-saturated juvenile hormone IIIManduca sexta-wild-type, at 30°C in 50 mM sodium phosphate buffer, pH 7.4, containing 1 mg/mL of bovine serum albumin707525-
0.000641-saturated juvenile hormone IIIManduca sexta-mutant F259I, at 30°C in 50 mM sodium phosphate buffer, pH 7.4, containing 1 mg/mL of bovine serum albumin707525-
3.7-methyl farnesoateDrosophila melanogasterA1ZA98-680156 2D-image
additional information-additional informationSpodoptera frugiperda, Spodoptera frugiperda Sf-21, Spodoptera frugiperda Sf9-of thioester compounds as surrogate substrates23491-
additional information-additional informationManduca sexta--23494-
additional information-additional informationGryllus assimilis-Km-values for juvenile hormone III from lines selected for high or low JHE activity649644-
additional information-additional informationDrosophila melanogaster, Drosophila melanogaster AANAT2, Drosophila melanogaster Canton S, Drosophila melanogaster CuZn-SOD, Drosophila melanogaster MH19, Drosophila melanogaster Oregon K, Drosophila melanogaster OregonR, Drosophila melanogaster Oregon R, Drosophila melanogaster Oregon-R, Drosophila melanogaster S-2, Drosophila melanogaster Samarkand-kinetic characterization651500-
additional information-additional informationRhyparobia maderae-kinetics651504-

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
22-Alpha-naphthyl acetateManduca sexta-wild-type, at 30°C in 50 mM sodium phosphate buffer, pH 7.4, containing 1 mg/mL of bovine serum albumin707525 2D-image
0.1-Juvenile hormoneRhyparobia maderae-during vitellogenesis, kcat at ovulation time is 2-3times higher than earlier during vitellogenesis651504 2D-image
0.39-Juvenile hormoneRhyparobia maderae-at ovulation time, kcat at ovulation time is 2-3times higher than earlier during vitellogenesis651504 2D-image
0.618-juvenile hormone ITrichoplusia ni--23477 2D-image
1.6-juvenile hormone IDrosophila melanogasterA1ZA98-680156 2D-image
0.0130.018juvenile hormone IIIBombyx moriD2KWC2pH 7.4, 22°C, recombinant BmCCE-5715173 2D-image
0.164-juvenile hormone IIITribolium castaneumD7US45pH and temperature not specified in the publication715184 2D-image
0.323-juvenile hormone IIITrichoplusia ni--23477 2D-image
0.7-juvenile hormone IIIManduca sexta-wild-type, at 30°C in 50 mM sodium phosphate buffer, pH 7.4, containing 1 mg/mL of bovine serum albumin707525 2D-image
0.8-juvenile hormone IIIDrosophila melanogasterA1ZA9810S isomer of juvenile hormone III680156 2D-image
0.9-juvenile hormone IIILeptinotarsa decemlineata-pH 8, major enzyme form with pI 5.6649498 2D-image
1-juvenile hormone IIIDrosophila melanogasterA1ZA98racemic juvenile hormone III680156 2D-image
1-juvenile hormone IIIBombyx moriD2KWC2pH 7.4, 22°C, recombinant BmJHE715173 2D-image
1-juvenile hormone IIIBombyx mori 122, Bombyx mori Bm-N, Bombyx mori C108, Bombyx mori P21, Bombyx mori P22, Bombyx mori Shogetu-Shunrei-pH 7.4, 22°C, recombinant BmJHE715173 2D-image
1.1-juvenile hormone IIIDrosophila melanogasterA1ZA9810R isomer of juvenile hormone III680156 2D-image
1.9-juvenile hormone IIILeptinotarsa decemlineata-pH 8, minor enzyme form with pI 5.5649498 2D-image
4-juvenile hormone IIIManduca sexta-mutant F259I, at 30°C in 50 mM sodium phosphate buffer, pH 7.4, containing 1 mg/mL of bovine serum albumin707525 2D-image
4.5-juvenile hormone IIIManduca sexta-mutant T314V, at 30°C in 50 mM sodium phosphate buffer, pH 7.4, containing 1 mg/mL of bovine serum albumin707525 2D-image
3-methyl farnesoateDrosophila melanogasterA1ZA98-680156 2D-image
0.7-saturated juvenile hormone IIIManduca sexta-wild-type, at 30°C in 50 mM sodium phosphate buffer, pH 7.4, containing 1 mg/mL of bovine serum albumin707525-
3.1-saturated juvenile hormone IIIManduca sexta-mutant F259I, at 30°C in 50 mM sodium phosphate buffer, pH 7.4, containing 1 mg/mL of bovine serum albumin707525-

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
53.65-Alpha-naphthyl acetateManduca sexta-wild-type, at 30°C in 50 mM sodium phosphate buffer, pH 7.4, containing 1 mg/mL of bovine serum albumin7075256899
22000-juvenile hormone IIIManduca sexta-wild-type, at 30°C in 50 mM sodium phosphate buffer, pH 7.4, containing 1 mg/mL of bovine serum albumin70752521521
40000-juvenile hormone IIIManduca sexta-mutant F259I, at 30°C in 50 mM sodium phosphate buffer, pH 7.4, containing 1 mg/mL of bovine serum albumin70752521521
53000-juvenile hormone IIIManduca sexta-mutant T314V, at 30°C in 50 mM sodium phosphate buffer, pH 7.4, containing 1 mg/mL of bovine serum albumin70752521521
4800-saturated juvenile hormone IIIManduca sexta-mutant F259I, at 30°C in 50 mM sodium phosphate buffer, pH 7.4, containing 1 mg/mL of bovine serum albumin7075250
14000-saturated juvenile hormone IIIManduca sexta-wild-type, at 30°C in 50 mM sodium phosphate buffer, pH 7.4, containing 1 mg/mL of bovine serum albumin7075250

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.0016-1,1,1-trifluoro-3-(octane-1-sulfonyl)propan-2,2-diolTenebrio molitorQ8WRD8IC50: 0.0016 mM651512 2D-image
0.00000099-1,1,1-trifluoro-3-decylsulfanyl-propan-2-oneHeliothis virescens-IC50: 0.99 nM, juvenile hormone III hydrolysis651510 2D-image
0.0000028-1,1,1-trifluoro-3-decylsulfanyl-propan-2-oneManduca sexta-IC50: 2.8 nM, juvenile hormone III hydrolysis651510 2D-image
0.0000036-1,1,1-trifluoro-3-decylsulfanyl-propan-2-oneTenebrio molitor-IC50: 3.6 nM, juvenile hormone III hydrolysis651510 2D-image
0.0000036-1,1,1-trifluoro-3-decylsulfanyl-propan-2-oneTenebrio molitorQ8WRD8potent inhibitor, IC50: 3.6 nm651512 2D-image
0.0000022-1,1,1-trifluoro-3-dodecylsulfanyl-propan-2-oneHeliothis virescens-IC50: 2.2 nM, juvenile hormone III hydrolysis651510 2D-image
0.0000044-1,1,1-trifluoro-3-dodecylsulfanyl-propan-2-oneManduca sexta-IC50: 4.4 nM, juvenile hormone III hydrolysis651510 2D-image
0.000012-1,1,1-trifluoro-3-dodecylsulfanyl-propan-2-oneTenebrio molitor-IC50: 12 nM, juvenile hormone III hydrolysis651510 2D-image
0.000014-1,1,1-trifluoro-3-hexylsulfanyl-propan-2-oneTenebrio molitor-IC50: 14 nM, juvenile hormone III hydrolysis651510 2D-image
0.0011-1,1,1-trifluoro-3-hexylsulfanyl-propan-2-oneHeliothis virescens-IC50: 1100 nM, juvenile hormone III hydrolysis651510 2D-image
0.0017-1,1,1-trifluoro-3-hexylsulfanyl-propan-2-oneManduca sexta-IC50: 1700 nM, juvenile hormone III hydrolysis651510 2D-image
0.0000046-1,1,1-trifluoro-3-octylsulfanyl-propan-2-oneTenebrio molitor-IC50: 4.6 nM, juvenile hormone III hydrolysis651510 2D-image
0.0000046-1,1,1-trifluoro-3-octylsulfanyl-propan-2-oneTenebrio molitorQ8WRD8potent inhibitor, IC50: 4.6 nm651512 2D-image
0.0000058-1,1,1-trifluoro-3-octylsulfanyl-propan-2-oneHeliothis virescens-IC50: 5.8 nM, juvenile hormone III hydrolysis651510 2D-image
0.0000091-1,1,1-trifluoro-3-octylsulfanyl-propan-2-oneManduca sexta-IC50: 9.1 nM, juvenile hormone III hydrolysis651510 2D-image
0.000036-1,1,1-trifluoro-3-phenethylsulfanyl-propan-2-oneTenebrio molitor-IC50: 36 nM, juvenile hormone III hydrolysis651510 2D-image
0.000075-1,1,1-trifluoro-3-phenethylsulfanyl-propan-2-oneHeliothis virescens-IC50: 75 nM, juvenile hormone III hydrolysis651510 2D-image
0.0004-1,1,1-trifluoro-3-phenethylsulfanyl-propan-2-oneManduca sexta-IC50: 400 nM, juvenile hormone III hydrolysis651510 2D-image
0.000022-3-Octylthio-1,1,1-trifluoropropan-2-oneManduca sextaP19985transition state analogue inhibitor, IC50 (wild type): 22 nM667723 2D-image
0.00033-3-Octylthio-1,1,1-trifluoropropan-2-oneManduca sextaP19985IC50 (F259I mutant): 330 nM667723 2D-image
0.1-CarbarylTenebrio molitorQ8WRD8weak, IC50: above 0.1 mM651512 2D-image
1-methamidophosTenebrio molitorQ8WRD8weak, IC50: above 1 mM651512 2D-image
0.0084-O-ethyl-S-phenyl-phosphoramidothiolateTenebrio molitorQ8WRD8IC50: 0.0084 mM651512 2D-image
0.24-PhenylmethylsulfonylfluorideTenebrio molitorQ8WRD8mediocre inhibitor, IC50: 0.24 mM651512 2D-image

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
0.0124-Leptinotarsa decemlineata-pH 8, fourth larval instar, at day 9 after molt649498
0.0135-Leptinotarsa decemlineata-pH 8, highest specific activity in the fourth larval instar, at day 3 after molt649498
0.179-Trichoplusia ni--23486
0.573-Manduca sexta-juvenile hormone III23494
0.9-Tenebrio molitorQ8WRD8recombinant JHE, hydrolysis of alpha-naphthyl acetate651512
1.1-Tenebrio molitorQ8WRD8recombinant JHE, hydrolysis of juvenile hormone III651512
1.841-Leptinotarsa decemlineata-pH 8, purified JHE from the fourth larval instar, at day 3 after molt649498
5.3-Tenebrio molitorQ8WRD8recombinant JHE, hydrolysis of p-nitrophenyl acetate651512
15.1-Tenebrio molitorQ8WRD8recombinant JHE, hydrolysis of HEPTAT651512
350-Tenebrio molitor-purified JHE from pupa, pH 8, 30°C649497
500000-Manduca sexta-purified wild-type, at 30°C in 50 mM sodium phosphate buffer, pH 7.4, containing 0.0005 mM juvenile hormone III and 1 mg/mL of bovine serum albumin707525
1050000-Manduca sexta-purified wild-type, at 30°C in 50 mM sodium phosphate buffer, pH 7.4, containing 0.0005 mM juvenile hormone III diol and 1 mg/mL of bovine serum albumin707525
2370000-Manduca sexta-purified mutant F259I, at 30°C in 50 mM sodium phosphate buffer, pH 7.4, containing 0.0005 mM juvenile hormone III and 1 mg/mL of bovine serum albumin707525
4200000-Manduca sexta-purified mutant T314V, at 30°C in 50 mM sodium phosphate buffer, pH 7.4, containing 0.0005 mM juvenile hormone III and 1 mg/mL of bovine serum albumin707525
4880000-Manduca sexta-purified mutant T314V, at 30°C in 50 mM sodium phosphate buffer, pH 7.4, containing 0.0005 mM juvenile hormone III diol and 1 mg/mL of bovine serum albumin707525
additional information-Manduca sexta, Trichoplusia ni-assay procedure23472
additional information-Lymantria dispar--23474
additional information-Trichoplusia ni--23486
additional information-Manduca sexta--23494
additional information-Trichoplusia ni-rapid assay23495
additional information-Ips typographus-specific activities of several developmental stages for the substrates juvenile hormone III, alpha-naphthyl acetate and methyl hexylthio acetothioate649497
additional information-Tenebrio molitor--649497
additional information-Leptinotarsa decemlineata-specific activities of different developmental stages649498
additional information-Gryllus assimilis--649644
additional information-Bombyx mori 122, Bombyx mori Bm-N, Bombyx mori C108, Bombyx mori, Bombyx mori P21, Bombyx mori P22, Bombyx mori Shogetu-Shunrei, Tribolium freemani-JHE activity at various larval and pupal stadia651397
additional information-Heliothis virescens, Manduca sexta, Tenebrio molitor-specific activities for several alpha-naphthyl and p-nitrophenyl substrates651510
additional information-Drosophila melanogaster AANAT2, Drosophila melanogaster Canton S, Drosophila melanogaster CuZn-SOD, Drosophila melanogaster, Drosophila melanogaster MH19, Drosophila melanogaster Oregon K, Drosophila melanogaster OregonR, Drosophila melanogaster Oregon R, Drosophila melanogaster Oregon-R, Drosophila melanogaster S-2, Drosophila melanogaster Samarkand-lower JHE-activity in pupae and prepupae of flies with impaired expression of JHE binding protein (DmP29) compared to control flies698082

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
6.59.5Galleria mellonella-juvenile hormone I23478
7-Reticulitermes flavipesD8UUL2assay at713975
7.1-Gryllus assimilis-assay at649644
7.4-Bombyx moriD2KWC2assay at; assay at715173
7.4-Apis mellifera-assay at715819
8-Ips typographus, Tenebrio molitor-assay at649497
8-Leptinotarsa decemlineata-assay at649498

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
5-Tenebrio molitorQ8WRD8the activity decreases significantly below pH 5651512
5.210.8Tenebrio molitorQ8WRD8active at a wide range of pH, at least 80% of the maximum activity in the pH-range651512

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
22-Bombyx mori 122-assay at715173
22-Bombyx moriD2KWC2assay at; assay at715173
22-Bombyx mori Bm-N, Bombyx mori C108, Bombyx mori P21, Bombyx mori P22, Bombyx mori Shogetu-Shunrei-assay at715173
30-Manduca sexta, Trichoplusia ni-assay at23472
30-Ips typographus, Tenebrio molitor-assay at649497
30-Gryllus assimilis-assay at649644
30-Tribolium freemani-assay at651397
30-Heliothis virescens, Manduca sexta, Tenebrio molitor, Trichoplusia ni-assay at651510
30-Reticulitermes flavipesD8UUL2assay at713975
30-Apis mellifera-assay at715819
35-Bombyx mori 122, Bombyx mori, Bombyx mori Bm-N, Bombyx mori C108, Bombyx mori P21, Bombyx mori P22, Bombyx mori Shogetu-Shunrei-assay at651397

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
4.34-Ips typographus, Tenebrio molitor-isoelectric focusing649497
4.9-Tenebrio molitorQ8WRD8recombinant JHE, isoelectric focusing, pH-gradient: pH 3-7651512
5.1-Lymantria dispar-two isoelectric forms, isoelectric focusing651502
5.3-Lymantria dispar-two isoelectric forms, isoelectric focusing651502
5.3-Tenebrio molitorQ8WRD8recombinant JHE, theoretical pI651512
5.5-Leptinotarsa decemlineata-two enzyme forms, minor form, isoelectric focusing, pH-gradient of 4-6.5649498
5.65.9Leptinotarsa decemlineata-two enzyme forms, major form, isoelectric focusing, pH-gradient of 4-6.5649498
6-Manduca sexta-isoelectric focusing651511
6.13-Apis melliferaQ76LA5theoretical697457

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
adultIps typographus-whole body649497Manually annotated by BRENDA team
CF-203 cellChoristoneura fumiferana--653222Manually annotated by BRENDA team
eggTrichoplusia ni-highest activity669746Manually annotated by BRENDA team
epidermisManduca sexta-dorsal abdominal epidermis of the last instar larvae23471Manually annotated by BRENDA team
fat bodyGalleria mellonella, insecta, Lymantria dispar, Manduca sexta, Neobellieria bullata, Periplaneta brunnea, Samia cynthia, Schistocerca nitens, Trichoplusia ni--23470Manually annotated by BRENDA team
fat bodyTenebrio molitorQ8WRD8of 0-48 hours old pupae651512Manually annotated by BRENDA team
fat bodyGryllus assimilisA5HSI6-680154Manually annotated by BRENDA team
fat bodyPsacothea hilarisQ1HA48expressed mainly in the fat body680155Manually annotated by BRENDA team
fat bodyLymantria disparQ7M4I4parasitism strongly suppresses host JHE activity in both hemolymph and fat body irrespective of whether the host is parasitized early (premolt-third instar) or late (mid-fourth instar)681237Manually annotated by BRENDA team
fat bodyDysdercus cingulatus--708700Manually annotated by BRENDA team
fat bodyApis mellifera--715819Manually annotated by BRENDA team
foregutReticulitermes flavipesD8UUL2-713975Manually annotated by BRENDA team
gutBombyx moriD2KWC2strong expression of Bmcce-5; strong expression of Bmcce-5715173Manually annotated by BRENDA team
gutBombyx mori 122, Bombyx mori Bm-N, Bombyx mori C108, Bombyx mori P21, Bombyx mori P22, Bombyx mori Shogetu-Shunrei-strong expression of Bmcce-5715173Manually annotated by BRENDA team
hemolymphinsecta, Lymantria dispar, Neobellieria bullata, Periplaneta brunnea, Samia cynthia, Schistocerca nitens--23470Manually annotated by BRENDA team
hemolymphHelicoverpa zea--23475Manually annotated by BRENDA team
hemolymphHeliothis virescens--23473, 23475Manually annotated by BRENDA team
hemolymphGalleria mellonella-of fully grown larvae23478Manually annotated by BRENDA team
hemolymphManduca sexta--23470, 23482Manually annotated by BRENDA team
hemolymphGalleria mellonella--23470, 23483Manually annotated by BRENDA team
hemolymphTrichoplusia ni-of larvae23486Manually annotated by BRENDA team
hemolymphTrichoplusia ni--23470, 23477, 23481, 23487Manually annotated by BRENDA team
hemolymphManduca sexta-of larvae23494Manually annotated by BRENDA team
hemolymphLeptinotarsa decemlineata-of last larval instar, enzyme occurs mainly in the hemolymph, JHE activities of hemolymph from animals of different developmental stages649498Manually annotated by BRENDA team
hemolymphGryllus assimilis-day 4-7 of the 10-day last stadium649644Manually annotated by BRENDA team
hemolymphBombyx mori, Bombyx mori 122, Bombyx mori Bm-N, Bombyx mori C108, Bombyx mori P21, Bombyx mori P22, Bombyx mori Shogetu-Shunrei-of fourth and fifth instars651397Manually annotated by BRENDA team
hemolymphLymantria dispar-of fifth instars651502Manually annotated by BRENDA team
hemolymphRhyparobia maderae--651504Manually annotated by BRENDA team
hemolymphTrichoplusia ni-of larvae at second day of the fifth instar651510Manually annotated by BRENDA team
hemolymphTenebrio molitorQ8WRD8the cDNA contains an independent secretory signal peptide and is likely to represent a specific isoform that would be present in the hemolymph in vivo651512Manually annotated by BRENDA team
hemolymphBombyx mori, Bombyx mori 122, Bombyx mori Bm-N, Bombyx mori C108, Bombyx mori P21, Bombyx mori P22, Bombyx mori Shogetu-Shunrei--670670Manually annotated by BRENDA team
hemolymphHelicoverpa zea-the highest levels of activity in day 2 virgin and mated females is found in ovaries followed by the carcass and then hemolymph681234Manually annotated by BRENDA team
hemolymphLymantria disparQ7M4I4parasitism strongly suppresses host JHE activity in both hemolymph and fat body irrespective of whether the host is parasitized early (premolt-third instar) or late (mid-fourth instar)681237Manually annotated by BRENDA team
hemolymphDysdercus cingulatus--708700Manually annotated by BRENDA team
hemolymphApis mellifera--715819Manually annotated by BRENDA team
hindgutReticulitermes flavipesD8UUL2-713975Manually annotated by BRENDA team
larvaTrichoplusia ni--23476, 23484Manually annotated by BRENDA team
larvaTrichoplusia ni-last larval instar23487Manually annotated by BRENDA team
larvaManduca sexta-plasma of day 3 fifth instar larvae23489Manually annotated by BRENDA team
larvaIps typographus-whole body649497Manually annotated by BRENDA team
larvaBombyx mori, Bombyx mori 122, Bombyx mori Bm-N, Bombyx mori C108, Bombyx mori P21, Bombyx mori P22, Bombyx mori Shogetu-Shunrei-JHE activity at various larval and pupal stadia, hemolymph of fourth and fifth instars, whole body of third instar651397Manually annotated by BRENDA team
larvaTribolium freemani-JHE activity at various larval and pupal stadia651397Manually annotated by BRENDA team
larvaTrichoplusia ni-second day of the fifth instar, hemolymph651510Manually annotated by BRENDA team
larvaBombyx moriQ6RVD1JHE mRNA is expressed in tissue-specific manners under the control of ecdysteroids and juvenile hormone677841Manually annotated by BRENDA team
larvaPsacothea hilarisQ1HA48PhJHE transcript levels are low until day 3 of the 5th (final) larval instar, then steadily increase reaching a peak on day 13 (the prepupa stage). Sustained starvation of larvae after a period of feeding stimulates the expression of PhJHE mRNA while feeding the larvae with glucose downregulates its expression680155Manually annotated by BRENDA team
larvaAedes aegyptiQ17AV1EAT43357 is detected during the late final instar larval stage. mRNA of EAT43357 is detected in both fat body and midgut tissues680159Manually annotated by BRENDA team
larvaLymantria disparQ7M4I4larva parasitized by Glyptapanteles liparidis. Parasitism strongly suppresses host JHE activity in both hemolymph and fat body irrespective of whether the host is parasitized early (premolt-third instar) or late (mid-fourth instar). Down-regulation of JHE activity is primarily due to the injection of PDV/venom at the time of oviposition, with only very small additive effects of teratocytes and wasp larvae under certain experimental conditions681237Manually annotated by BRENDA team
larvaApis melliferaQ76LA5maximum expression at end of larval feeding stage (F3) (qRT-PCR), high expression in fifth larval instar, at switch from feeding to early spinning-stage (S1) (lowest juvenile hormone titer), expression triggered by juvenile hormone (JH III) titer and suppressed by ecdysteroid (qRT-PCR), counterbalancing effect697457Manually annotated by BRENDA team
malpighian tubuleBombyx moriD2KWC2strong expression of Bmcce-5; strong expression of Bmcce-5715173Manually annotated by BRENDA team
midgutGryllus assimilisA5HSI6-680154Manually annotated by BRENDA team
moulting fluidManduca sexta-pharate pupae23480Manually annotated by BRENDA team
nymphDysdercus cingulatus-JHE is prominent only on the final day of 5th instar nymph708700Manually annotated by BRENDA team
ovaryHelicoverpa zea-the highest levels of activity in day 2 virgin and mated females is found in ovaries followed by the carcass and then hemolymph. For ovary the activity is highest in virgin compared to mated females681234Manually annotated by BRENDA team
pharate pupaManduca sexta-moulting fluid23480Manually annotated by BRENDA team
prepupaTenebrio molitor--649497Manually annotated by BRENDA team
prepupaDrosophila melanogaster, Drosophila melanogaster AANAT2, Drosophila melanogaster Canton S, Drosophila melanogaster CuZn-SOD, Drosophila melanogaster MH19, Drosophila melanogaster Oregon K, Drosophila melanogaster OregonR, Drosophila melanogaster Oregon R, Drosophila melanogaster Oregon-R, Drosophila melanogaster S-2, Drosophila melanogaster Samarkand-JHE from the prepupal stage651500Manually annotated by BRENDA team
prepupaDrosophila melanogaster, Drosophila melanogaster AANAT2, Drosophila melanogaster Canton S, Drosophila melanogaster CuZn-SOD, Drosophila melanogaster MH19, Drosophila melanogaster Oregon K, Drosophila melanogaster OregonR, Drosophila melanogaster Oregon R, Drosophila melanogaster Oregon-R, Drosophila melanogaster S-2, Drosophila melanogaster Samarkand--698082Manually annotated by BRENDA team
pupaIps typographus-when induced by topical application of juvenile hormone III649497Manually annotated by BRENDA team
pupaTenebrio molitor-whole body649497Manually annotated by BRENDA team
pupaBombyx mori, Bombyx mori 122, Bombyx mori Bm-N, Bombyx mori C108, Bombyx mori P21, Bombyx mori P22, Bombyx mori Shogetu-Shunrei, Tribolium freemani-JHE activity at various larval and pupal stadia651397Manually annotated by BRENDA team
pupaTenebrio molitorQ8WRD80-48 hours old, fat bodies651512Manually annotated by BRENDA team
pupaApis melliferaQ76LA5low expression in prepupa and during pupal stage (qRT-PCR)697457Manually annotated by BRENDA team
pupaDrosophila melanogaster, Drosophila melanogaster AANAT2, Drosophila melanogaster Canton S, Drosophila melanogaster CuZn-SOD, Drosophila melanogaster MH19, Drosophila melanogaster Oregon K, Drosophila melanogaster OregonR, Drosophila melanogaster Oregon R, Drosophila melanogaster Oregon-R, Drosophila melanogaster S-2, Drosophila melanogaster Samarkand-11 hours after puparation698082Manually annotated by BRENDA team
salivary glandReticulitermes flavipesD8UUL2-713975Manually annotated by BRENDA team
trunkHelicoverpa zea-the highest levels of activity in day 2 virgin and mated females is found in ovaries followed by the carcass and then hemolymph681234Manually annotated by BRENDA team
whole bodyHelicoverpa zea-homogenates of virgin and mated females from day 0 to day 8 post-emergence. Maximal activity for virgin females occurrs on day 2, which is approximately twice that of mated females on the same day. By day 4 activity declines significantly in virgin and mated females and is the same through day 7681234Manually annotated by BRENDA team
whole bodyApis melliferaQ76LA5nurse bee (5-10 day-old), highest level among worker bees (qRT-PCR), prevents switch to foraging (around day 15)697457Manually annotated by BRENDA team
midgutReticulitermes flavipesD8UUL2high expression level of gene RfEst1713975Manually annotated by BRENDA team
additional informationIps typographus-developmental profile of JHE activity with different substrates649497Manually annotated by BRENDA team
additional informationChoristoneura fumiferana-developmental expression pattern653222Manually annotated by BRENDA team
additional informationTrichoplusia ni-homogenates of all stages of development: egg, larva, pupa, and adult669746Manually annotated by BRENDA team
additional informationReticulitermes flavipesD8UUL2Est1 expression analysis in different gut sections, overview713975Manually annotated by BRENDA team
additional informationBombyx moriD2KWC2developmental expression patterns of gene Bmcce-5 by real-time quantitative PCR analysis, the expression pattern varies among developmental stages and organs715173Manually annotated by BRENDA team
additional informationTribolium castaneumD7US45expression of Tcjhe during development715184Manually annotated by BRENDA team

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
solubleTenebrio molitorQ8WRD8recombinant JHE-651512Manually annotated by BRENDA team
additional informationChoristoneura fumiferana-localization of mRNA in the sixth instar larvae-653222Manually annotated by BRENDA team
additional informationDrosophila melanogaster, Drosophila melanogaster AANAT2, Drosophila melanogaster Canton S, Drosophila melanogaster CuZn-SOD, Drosophila melanogaster MH19, Drosophila melanogaster Oregon K, Drosophila melanogaster OregonR, Drosophila melanogaster Oregon R, Drosophila melanogaster Oregon-R, Drosophila melanogaster S-2, Drosophila melanogaster Samarkand-transport mediated by JHE binding protein (DmP29)-698082Manually annotated by BRENDA team

PDBSCOPCATHORGANISM
No entries in this field

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
48000-Trichoplusia ni-Trichoplusia ni, gel filtration, gel electrophoresis23481
50000-Dysdercus cingulatus-native PAGE708700
60000-Galleria mellonella-Galleria mellonella23483
62000-Lymantria dispar-Lymantria dispar23474
64000-Apis melliferaQ76LA5theoretical, 567 amino acid polypeptide697457
65000-Trichoplusia ni-Trichoplusia ni, gel filtration23487
66000-Trichoplusia ni-Trichoplusia ni23476
66000-Manduca sexta-Manduca sexta, HPLC gel filtration23489
66000-Dysdercus cingulatus-native PAGE708700
120000-Leptinotarsa decemlineata-major form with pI 5.6, nondenaturing PAGE649498

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
?Heliothis virescens-x * 61012, Heliothis virescens, deduced from nucleotide sequence23488
?Manduca sexta-x * 65000, Manduca sexta, SDS-PAGE23494
?Lymantria dispar-x * 50000, SDS-PAGE651502
dimerLeptinotarsa decemlineata-2 * 57000, major form with pI 5.6, the subunits are not linked by disulfide bridges, SDS-PAGE649498
monomerTrichoplusia ni-1 * 48000, Trichoplusia ni, SDS-PAGE23481
monomerTrichoplusia ni-1 * 64000, Trichoplusia ni, SDS-PAGE23487
monomerManduca sexta-1 * 66000, Manduca sexta, SDS-PAGE23489
monomerTenebrio molitorQ8WRD81 * 66000, recombinant JHE, SDS-PAGE, 1 * 63600, recombinant JHE, sequence calculation651512

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
glycoproteinHeliothis virescens-JHE is highly glycosylated651510
no glycoproteinManduca sexta--651510
glycoproteinTrichoplusia ni--23487
glycoproteinTrichoplusia ni-location of glycosylation site23490

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
hanging drop method, 2.7 A crystal structure of the enzyme in complex with the transition state analogue inhibitor 3-octylthio-1,1,1-trifluoropropan-2-one covalently bound to the active site; in complex with 3-octylthio-1,1,1-trifluoropropan-2-one, hanging drop vapor diffusion methodManduca sextaP19985667723
recombinant JHE, complexed with 1,1,1-trifluoro-3-octylsulfanyl-propan-2-oneManduca sexta-651510

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
6.58.5Galleria mellonella-5°C, 14 h stable23478

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
5-Galleria mellonella-pH 6.5-8.5, 14 h stable23478
60.461.8Gryllus assimilis-5 min, 50% loss of activity, high- and low-selected JHE activity lines649644

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
Unstable when exposed to charged environments of low enzyme concentrationTrichoplusia ni-23481

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
AcetoneHeliothis virescens-stable, even at higher concentrations651510
AcetoneManduca sexta-stable in, activates even at higher concentrations651510
AcetoneTenebrio molitor-unstable, rapid decrease in activity at low concentrations651510
AcetoneTrichoplusia ni-stable in651510
EthanolHeliothis virescens-stable, even at higher concentrations651510
EthanolManduca sexta-stable in, activates even at higher concentrations651510
EthanolTenebrio molitor-unstable, rapid decrease in activity at low concentrations651510
isopropanolHeliothis virescens-stable, even at higher concentrations651510
isopropanolManduca sexta-stable in, activates even at higher concentrations651510
Triton X-100Heliothis virescens-stable, even at higher concentrations651510
Triton X-100Manduca sexta-stable in, activates at low concentrations651510
Triton X-100Tenebrio molitor-unstable, rapid decrease in activity at low concentrations651510
Triton X-100Trichoplusia ni-stable in651510
isopropanolTenebrio molitor-unstable, rapid decrease in activity at low concentrations651510
additional informationManduca sexta-remarkably resistant to the addition of organic co-solvents and detergent651510
additional informationManduca sexta-high tolerance of organic solvents, organic solvents activate at lower concentrations, JHE remains significantly active even at high concentrations651511

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-20°C, stable for 2 monthsGalleria mellonella-23478
5°C, pH 6.5-8.5, 14 h stableGalleria mellonella-23478
storage on ice, 0.1 M phosphate buffer, pH 7.1, up to 3 weeks, stableGryllus assimilis-649644
-70°C, stableTrichoplusia ni-23486
stable in 40% sucrose for 5 monthsTrichoplusia ni-23481

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-Bombyx mori, Bombyx mori 122, Bombyx mori Bm-N, Bombyx mori C108, Bombyx mori P21, Bombyx mori P22, Bombyx mori Shogetu-Shunrei-23492
about 429foldDrosophila melanogaster, Drosophila melanogaster AANAT2, Drosophila melanogaster Canton S, Drosophila melanogaster CuZn-SOD, Drosophila melanogaster MH19, Drosophila melanogaster Oregon K, Drosophila melanogaster Oregon R, Drosophila melanogaster Oregon-R, Drosophila melanogaster OregonR, Drosophila melanogaster S-2, Drosophila melanogaster Samarkand-651500
purified preparation reveals 2 distinct bands: the heavy band does not contain any detectable 1-naphthylacetate activity and is comprised of 3 closely positioned protein bands, the second band exhibits 1-naphthylacetate esterase activity in addition to juvenile hormone esterase activityGalleria mellonella-23478
-Helicoverpa zea, Heliothis virescens-23475, 23492
recombinant enzyme secreted into the cell medium in a baculovirus expression system, affinity chromatography with 8-mercapto-1,1,1-trifluoro-2-octanoneHeliothis virescensP1299223473
recombinant JHEHeliothis virescens-651510
147fold, from the fourth larval instar, at day 3 after moltLeptinotarsa decemlineata-649498
-Lymantria dispar-651502
-Manduca sexta-23482, 23492, 23494
affinity chromatography with the ligand 3-[(4'-mercapto)butylthio]-1,1,1-trifluoropropan-2-oneManduca sexta-23489
expressed wild-type and mutant proteins purified by anion-exchange chromatography. Purification efficiencies of wild-type JHE, mutant F259I, and mutant T314V are 77%, 32%, and 51%, respectively and are purified 4-, 4.4-, and 4.5fold, respectivelyManduca sexta-707525
recombinant JHEManduca sexta-651510
recombinant JHE, partialManduca sexta-651511
recombinant proteinManduca sextaP19985667723
-Rhyparobia maderae-651504
from pupa, 49foldTenebrio molitor-649497
recombinant JHETenebrio molitor-651510
recombinant JHE, partial, 2.5foldTenebrio molitorQ8WRD8651512
-Trichoplusia ni-23476, 23481, 23486, 23487

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
EAT43357 cDNA is expressed in a baculovirus system. Proteins isolated from Sf9 cells infected with recombinant baculovirus expressing EAT43357 gene exhibit JHE activityAedes aegyptiQ17AV1680159
expressed in the Bombyx mori nondiapause strain pnd-w1Bombyx mori-670670
gene Bmcce-5, DNA and amino acid sequence determination and analysis, intron-exon structures, sequence comparisons and phylogenetic analysis, recombinant expression of BmCCE-5 as C-terminal V5-His6-tagged protein in Spodoptera frugiperda Sf9 cells using the baculovirus system; gene Bmjhe, DNA and amino acid sequence determination and analysis, intron-exon structures, sequence comparisons and phylogenetic analysis of JHE, recombinant expression of BmJHE as C-terminal V5-His6-tagged protein in Spodoptera frugiperda Sf9 cells using the baculovirus systemBombyx moriD2KWC2715173
expressed in the Bombyx mori nondiapause strain pnd-w1Bombyx mori 122-670670
gene Bmjhe, DNA and amino acid sequence determination and analysis, intron-exon structures, sequence comparisons and phylogenetic analysis of JHE, recombinant expression of BmJHE as C-terminal V5-His6-tagged protein in Spodoptera frugiperda Sf9 cells using the baculovirus systemBombyx mori 122-715173
expressed in the Bombyx mori nondiapause strain pnd-w1Bombyx mori Bm-N-670670
gene Bmjhe, DNA and amino acid sequence determination and analysis, intron-exon structures, sequence comparisons and phylogenetic analysis of JHE, recombinant expression of BmJHE as C-terminal V5-His6-tagged protein in Spodoptera frugiperda Sf9 cells using the baculovirus systemBombyx mori Bm-N-715173
expressed in the Bombyx mori nondiapause strain pnd-w1Bombyx mori C108-670670
gene Bmjhe, DNA and amino acid sequence determination and analysis, intron-exon structures, sequence comparisons and phylogenetic analysis of JHE, recombinant expression of BmJHE as C-terminal V5-His6-tagged protein in Spodoptera frugiperda Sf9 cells using the baculovirus systemBombyx mori C108-715173
expressed in the Bombyx mori nondiapause strain pnd-w1Bombyx mori P21-670670
gene Bmjhe, DNA and amino acid sequence determination and analysis, intron-exon structures, sequence comparisons and phylogenetic analysis of JHE, recombinant expression of BmJHE as C-terminal V5-His6-tagged protein in Spodoptera frugiperda Sf9 cells using the baculovirus systemBombyx mori P21-715173
expressed in the Bombyx mori nondiapause strain pnd-w1Bombyx mori P22-670670
gene Bmjhe, DNA and amino acid sequence determination and analysis, intron-exon structures, sequence comparisons and phylogenetic analysis of JHE, recombinant expression of BmJHE as C-terminal V5-His6-tagged protein in Spodoptera frugiperda Sf9 cells using the baculovirus systemBombyx mori P22-715173
expressed in the Bombyx mori nondiapause strain pnd-w1Bombyx mori Shogetu-Shunrei-670670
gene Bmjhe, DNA and amino acid sequence determination and analysis, intron-exon structures, sequence comparisons and phylogenetic analysis of JHE, recombinant expression of BmJHE as C-terminal V5-His6-tagged protein in Spodoptera frugiperda Sf9 cells using the baculovirus systemBombyx mori Shogetu-Shunrei-715173
expression in the baculovirus expression systemChoristoneura fumiferana-653222
-Dysdercus cingulatus-708700
-Gryllus assimilisA5HSI6680154
-Heliothis virescens-23473, 23488
expression in High 5 cellsHeliothis virescens, Manduca sexta-651510
full-length cDNA, expression in Hi5 cells from Trichoplusia niManduca sexta-651511
native and mutant enzyme expressed in High 5 cellsManduca sextaP19985667723
recombinant baculovirus AcMsJHE-F259I expressing mutant F259I, recombinant baculovirus AcMsJHE-T314V or recombinant baculovirus pAcUW21-T314V expressing mutant T314V. AcMsJHE-T314V generated by transfection of Sf-21 cells with pAcUW21-T314V and linearized BacPAK6 baculovirus DNA. Wild-type JHE, mutants F259I and T314V produced in insect High Five cells cultured in ESF 921 medium that are inoculated with AcMsJHE7, AcMsJHE-F259I, or AcMsJHE-T314V, respectivelyManduca sexta-707525
-Psacothea hilarisQ1HA48680155
gene RfEst1, cloning from gut cDNA library, DNA and amino acid sequence determination and analysis, sequence comparisons, expression analysisReticulitermes flavipesD8UUL2713975
expression in High 5 cellsTenebrio molitor-651510
from fat bodies of pupae, full-length cDNA, expression in Hi-5 cells from Trichoplusia ni, sequencingTenebrio molitorQ8WRD8651512
genes Tcjhe-a, Tcjhe-b, Tcjhe-c, Tcjhe-d, and Tcjhe-e, encoding isozymes that vary only in the 5'-UTR sequences, DNA and amino acid sequence determination and analysis, intron-exon structure, phylogenetic analysis, overview, recombinant expression of JHE as C-terminal V5-His6-tagged protein in Spodoptera frugiperda Sf9 cells using the baculovirus systemTribolium castaneumD7US45715184

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
F259IManduca sextaP19985lower affinity for 3-octylthio-1,1,1-trifluoropropan-2-one667723
F259IManduca sexta-the overall structure of the substrate binding pocket is similar to that of wild-type JHE. Shows a 5.7fold higher kcat for juvenile hormone III as the wild-type. KM of mutant F259I for juvenile hormone III is 3.4fold higher than that of the wild-type. With the saturated form of juvenile hormone III as a substrate, mutant F259I shows a 4.4fold higher kcat and 14fold higher Km in comparison to the wild-type707525
T314VManduca sexta-no major changes in the overall structure of the substrate binding pocket. The KM of mutant T314V for juvenile hormone III is 2.9fold higher than that of JHE, whereas kcat is 6.4fold higher. Specific activity of mutant T314V for juvenile hormone III diol is not statistically different than that of wild-type for juvenile hormone III707525

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
synthesisManduca sexta-the high tolerance of organic solvents may make JHE useful in future applications as a synthetic catalyst651511

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISM (UNIPROT ACCESSION NO.)LINK TO PUBMEDSOURCE
23470De Kort, C.A.D.; Granger, N.A.Regulation of the juvenile hormone titerAnn. Rev. Entomol.261-281981Galleria mellonella, insecta, Lymantria dispar, Manduca sexta, Neobellieria bullata, Periplaneta brunnea, Samia cynthia, Schistocerca nitens, Trichoplusia ni-
23471Mitsui, T.; Riddiford, L.M.; Bellamy, G.Metabolism of juvenile hormone by the epidermis of the tobacco hornworm, Manduca sextaInsect Biochem.9637-6431979Manduca sexta-
23472Hammock, B.D.; Roe, R.M.Analysis of juvenile hormone esterase activityMethods Enzymol.111487-4941985Manduca sexta, Trichoplusia ni PubMed
23473Shiotsuki, T.; Huang, T.L.; Uematsu, T.; Bonning, B.C.; Ward, V.K.; Hammock, B.D.Juvenile hormone esterase purified by affinity chromatography with 8-mercapto-1,1,1-trifluoro-2-octanone as a rationally designed ligandProtein Expr. Purif.5296-3061994Heliothis virescens, Heliothis virescens (P12992) PubMed
23474Valaitis, A.P.Characterization of hemolymph juvenile hormone esterase from Lymantria disparInsect Biochem.21583-5951991Lymantria dispar-
23475Abdel-Aal, Y.A.I.; Hanzlik, T.N.; Hammock, B.D.; Harshman, L.G.; Prestwich, G.Juvenile hormone esterases in two heliothines: kinetic, biochemical and immunogenic characterizationComp. Biochem. Physiol. B Comp. Biochem.90117-1241988Helicoverpa zea, Heliothis virescens (P12992)-
23476Rudnicka, M.; Jones, D.Characterization of homogeneous juvenile hormone esterase from larvae of Trichoplusia niInsect Biochem.17373-3821987Trichoplusia ni-
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23478Rudnicka, M.; Kochman, M.Purification of the juvenile hormone esterase from the hemolymph of the wax moth Galleria mellonella (Lepidoptera)Insect Biochem.14189-1981984Galleria mellonella-
23479Sparks, T.C.; Rose, R.L.Inhibition and substrate specificity of the hemolymph juvenile hormone esterase of the cabbage looper, Trichoplusia ni (Huebner)Insect Biochem.13633-6401983Trichoplusia ni-
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23483McCaleb, D.C.; Reddy, G.; Kumaran, A.K.Some properties of the hemolymph juvenile hormone esterases in Galleria mellonella larvae and Tenebrio molitor pupaeInsect Biochem.10273-2771980Galleria mellonella, Tenebrio molitor-
23484Sparks, T.C.; Hammock, B.D.A comparison of the induced and naturally occurring juvenile hormone esterases from last instar larvae of Trichoplusia niInsect Biochem.9411-4211979Trichoplusia ni-
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651397Hirashima, A.; Suetsugu, E.; Hirokado, S.; Kuwano, E.; Taniguchi, E.; Eto, M.Effect of octopamine on the activity of juvenile-hormone esterase in the silkworm Bombyx mori and the red flour beetle Tribolium freemaniGen. Comp. Endocrinol.116373-3811999Bombyx mori, Bombyx mori Shogetu-Shunrei, Tribolium freemani PubMed
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653222Feng, Q.L.; Ladd, T.R.; Tomkins, B.L.; Sundaram, M.; Sohi, S.S.; Retnakaran, A.; Davey, K.G.; Palli, S.R.Spruce budworm (Choristoneura fumiferana) juvenile hormone esterase: hormonal regulation, developmental expression and cDNA cloningMol. Cell. Endocrinol.14895-1081999Choristoneura fumiferana PubMed
667723Wogulis, M.; Wheelock, C.E.; Kamita, S.G.; Hinton, A.C.; Whetstone, P.A.; Hammock, B.D.; Wilson, D.K.Structural studies of a potent insect maturation inhibitor bound to the juvenile hormone esterase of Manduca sextaBiochemistry454045-40572006Manduca sexta, Manduca sexta (P19985), Manduca sexta (Q9GPG0) PubMed
669746Anspaugh, D.D.; Roe, R.M.Regulation of JH epoxide hydrolase versus JH esterase activity in the cabbage looper, Trichoplusia ni, by juvenile hormone and xenobioticsJ. Insect Physiol.51523-5352005Trichoplusia ni PubMed
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677841Kamimura, M.; Takahashi, M.; Kikuchi, K.; Reza, A.M.; Kiuchi, M.Tissue-specific regulation of juvenile hormone esterase gene expression by 20-hydroxyecdysone and juvenile hormone in Bombyx moriArch. Insect Biochem. Physiol.65143-1512007Bombyx mori, Bombyx mori (Q6RVD1) PubMed
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698082Liu, Z.; Li, X.; Prasifka, J.R.; Jurenka, R.; Bonning, B.C.Overexpression of Drosophila juvenile hormone esterase binding protein results in anti-JH effects and reduced pheromone abundanceGen. Comp. Endocrinol.156164-1722008Drosophila melanogaster PubMed
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713975Wheeler, M.; Tarver, M.; Coy, M.; Scharf, M.Characterization of four esterase genes and esterase activity from the gut of the termite Reticulitermes flavipesArch. Insect Biochem. Physiol.7330-482010Reticulitermes flavipes, Reticulitermes flavipes (D8UUL2) PubMed
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715819Mackert, A.; Hartfelder, K.; Bitondi, M.M.; Simoes, Z.L.The juvenile hormone (JH) epoxide hydrolase gene in the honey bee (Apis mellifera) genome encodes a protein which has negligible participation in JH degradationJ. Insect Physiol.561139-11462010Apis mellifera PubMed

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