Information on EC 2.7.7.9 - UTP-glucose-1-phosphate uridylyltransferase:
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EC NUMBERCOMMENTARY
2.7.7.9-

RECOMMENDED NAMEGeneOntology No.
UTP-glucose-1-phosphate uridylyltransferaseGO:0003983

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose
show the reaction diagram		----
UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose
show the reaction diagram		sequential ordered bi bi reaction mechanismMus musculus-643726
UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose
show the reaction diagram		sequential ordered bi bi reaction mechanismSorghum bicolor-643743
UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose
show the reaction diagram		mechanismAcetabularia sp., Beta vulgaris subsp. vulgaris var. altissima, Bombyx mori, Bos taurus, Canis lupus familiaris, Capra hircus, Columba sp., Dictyostelium discoideum, Escherichia coli, Gallus gallus, Homo sapiens, Oryctolagus cuniculus, Ovis aries, Pisum sativum, Rattus norvegicus, Saccharomyces cerevisiae, Salmonella enterica subsp. enterica serovar Typhimurium, Zea mays-643748
UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose
show the reaction diagram		Trp333 and Arg391 are essential for activity, while His266, Arg389, Arg422, Arg 445, and Trp218 are notHomo sapiens-643751
UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose
show the reaction diagram		sequential ordered bi bi reaction mechanismHordeum vulgare subsp. vulgare-643752
UTP + alpha-D-glucose 1-phosphate = diphosphate + UDP-glucose
show the reaction diagram		ordered bi-bi mechanism. The molecular geometry at position 4 of glucose is responsible for substrate specificity. The gamma-phosphate group of UTP is essential for binding and for induction of the open conformation, which then allows entry of glucose 1-phosphateLeishmania majorQ4QDU3674571

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
nucleotidyl group transfer----

PATHWAYKEGG LinkMetaCyc Link
chitin biosynthesis-PWY-6981
colanic acid building blocks biosynthesis-COLANSYN-PWY
galactose degradation III-PWY-3821
glycogen biosynthesis II (from UDP-D-Glucose)-PWY-5067
L-ascorbate biosynthesis VI-PWY3DJ-35471
stachyose degradation-PWY-6527
sucrose biosynthesis-SUCSYN-PWY
sucrose degradation III-PWY-621
sucrose degradation VI (anaerobic)-PWY-3801

SYSTEMATIC NAMEIUBMB Comments
UTP:alpha-D-glucose-1-phosphate uridylyltransferase-

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
GalUAeromonas hydrophila--675852
glucose 1-phosphate uridylyltransferase----
glucose-1-phosphate uridylyltransferase----
UDP glucose pyrophosphorylase----
UDP-glucose pyrophosphorylase----
UDP-glucose pyrophosphorylaseArabidopsis thaliana-; 700488, 702416
UDP-glucose pyrophosphorylaseHordeum vulgare--702453
UDP-glucose pyrophosphorylaseMycobacterium tuberculosis H37RvO05576-706591
UDP-glucose pyrophosphorylase isoenzyme UGP5Solanum tuberosum--705362
UDP-glucose pyrophosphorylase UGP1Arabidopsis thaliana--706183
UDP-glucose pyrophosphorylase UGP2Arabidopsis thaliana--706183
UDP-glucose pyrophosphorylase UGP3Arabidopsis thaliana--706224
UDPG phosphorylase----
UDPG pyrophosphorylase----
UDPglucose pyrophosphorylase----
UDPglucose pyrophosphorylaseXanthomonas axonopodis pv. citriQ8PK83-702480
UDPglucose pyrophosphorylaseXanthomonas campestris pv. campestrisQ4UVD0-702480
UDPGPHevea brasiliensis--692142
UGPaseSolanum tuberosum--643742
UGPaseSaccharomyces cerevisiae--643749
UGPaseHordeum vulgare--643753, 643757
UGPaseArabidopsis thaliana--643755
UGPG-PPaseGallus gallus--643756
uridine 5'-diphosphoglucose pyrophosphorylase----
uridine diphosphate-D-glucose pyrophosphorylase----
uridine diphosphate-glucose pyrophosphorylaseStreptococcus equi subsp. zooepidemicusQ8GQP9-701878
uridine diphosphoglucose pyrophosphorylase----
uridine-diphosphate glucose pyrophosphorylase----
uridylyltransferase, glucose 1-phosphate----
VldBStreptomyces hygroscopicus--675332

CAS REGISTRY NUMBERCOMMENTARY
9026-22-6-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Acanthamoeba castellaniiNeff643745--Manually annotated by BRENDA team
Acetabularia sp.-643748--Manually annotated by BRENDA team
Aeromonas hydrophila-675852--Manually annotated by BRENDA team
Arabidopsis thaliana-675432, 702416Q9M9P3UniProtManually annotated by BRENDA team
Arabidopsis thaliana-702416, 706183P57751UniProtManually annotated by BRENDA team
Arabidopsis thaliana-706183, 706224Q94B70UniProtManually annotated by BRENDA team
Arabidopsis thalianaecotype Columbia for wild-type, ecotype Bensheim for transgenic plants; gene Ugp, inducible by exogenous sucrose, light and cold stress643755--Manually annotated by BRENDA team
Arabidopsis thalianasyncytia induced by nematode Heterodera schachtii700488P57751, Q9M9P3UniProtManually annotated by BRENDA team
Arabidopsis thalianawild type, Pi-deficient mutant, Pi-excess mutant662716--Manually annotated by BRENDA team
Beta vulgaris subsp. vulgaris var. altissimasugar beet643748--Manually annotated by BRENDA team
Bifidobacterium bifidum-642817, 642818--Manually annotated by BRENDA team
Bombyx mori-643748--Manually annotated by BRENDA team
Bos taurus-643748--Manually annotated by BRENDA team
Bos tauruscow643730--Manually annotated by BRENDA team
Bos tauruscrystallized liver enzyme643736--Manually annotated by BRENDA team
Bos taurusmale calf643731--Manually annotated by BRENDA team
Canis lupus familiaris-643748--Manually annotated by BRENDA team
Capra hircus-643748--Manually annotated by BRENDA team
Columba sp.pigeon643748--Manually annotated by BRENDA team
Corynebacterium glutamicum-677027--Manually annotated by BRENDA team
Corynebacterium glutamicum-677027Q6M6R3UniProtManually annotated by BRENDA team
Cucumis meloin addition to UGPase, presence of dual substrate UDP-hexose pyrophosphorylase676614Q19TV8SwissProtManually annotated by BRENDA team
Cucumis sativuscv. Calypiso642819--Manually annotated by BRENDA team
Dictyostelium discoideum-643747, 643748--Manually annotated by BRENDA team
Entamoeba histolytica-642813--Manually annotated by BRENDA team
Escherichia coli-643748--Manually annotated by BRENDA team
Escherichia coli-677032P0AEP3UniprotManually annotated by BRENDA team
Escherichia coliseveral mutant strains, some derived from wild-type parent strain AB1133643738--Manually annotated by BRENDA team
Felis catusGolgi apparatus and cytosolic isozyme643734, 643735--Manually annotated by BRENDA team
Gallus gallus-643748, 643756--Manually annotated by BRENDA team
Helicobacter pyloristrain 26 695660635--Manually annotated by BRENDA team
Helicobacter pylori 26 695strain 26 695660635--Manually annotated by BRENDA team
Hevea brasiliensis-692142--Manually annotated by BRENDA team
Homo sapiens-642814, 643748, 643751--Manually annotated by BRENDA team
Homo sapienscrystallized liver enzyme643732, 643736--Manually annotated by BRENDA team
Hordeum vulgare-643753, 663047, 702453Q43772SwissProtManually annotated by BRENDA team
Hordeum vulgaregene Ugp643757Q43772SwissProtManually annotated by BRENDA team
Hordeum vulgare subsp. vulgare-643739, 643752--Manually annotated by BRENDA team
Leishmania major-674571Q4QDU3SwissProtManually annotated by BRENDA team
Leishmania major-674795--Manually annotated by BRENDA team
Lilium longiflorum-643729--Manually annotated by BRENDA team
Mus musculus-643726--Manually annotated by BRENDA team
Mus musculustransgenes PPL-1 and PPL-2662442--Manually annotated by BRENDA team
Mycobacterium tuberculosis H37Rv-706591O05576UniProtManually annotated by BRENDA team
Neurospora crassa-643725--Manually annotated by BRENDA team
Nicotiana tabacum-676407--Manually annotated by BRENDA team
Oryctolagus cuniculus-643733, 643748--Manually annotated by BRENDA team
Oryctolagus cuniculuscrystallized liver enzyme643736--Manually annotated by BRENDA team
Oryza sativa-643724--Manually annotated by BRENDA team
Oryza sativaisoform Ugp1676438A3QQQ3UniprotManually annotated by BRENDA team
Oryza sativaisoforms Ugp1 and Ugp2676348A3QQQ3UniprotManually annotated by BRENDA team
Ovis arieslamb643748--Manually annotated by BRENDA team
Pisum sativum-643748--Manually annotated by BRENDA team
Populus tremula x Populus tremuloidesisoforms UGP1, UGP2673839--Manually annotated by BRENDA team
Pseudomonas aeruginosa-663272--Manually annotated by BRENDA team
Rattus norvegicus-643748--Manually annotated by BRENDA team
Rattus norvegicusdifferent isoforms in different tissues643728--Manually annotated by BRENDA team
Saccharomyces cerevisiae-637084, 643748, 661732--Manually annotated by BRENDA team
Saccharomyces cerevisiaecommercial product643750--Manually annotated by BRENDA team
Saccharomyces cerevisiaegene UGP1, i.e. open reading frame YKL248643749--Manually annotated by BRENDA team
Saccharomyces cerevisiaeisoform Ugp1675416--Manually annotated by BRENDA team
Saccharomyces fragilisgalactose-adopted637084--Manually annotated by BRENDA team
Salmonella enterica subsp. enterica serovar Typhimurium-643746, 643748--Manually annotated by BRENDA team
Solanum tuberosum-643740, 643744--Manually annotated by BRENDA team
Solanum tuberosumcv. Norchip643116, 643742--Manually annotated by BRENDA team
Solanum tuberosumcv. Snowden MTs705362--Manually annotated by BRENDA team
Sorghum bicolorcv. Rox Orange643743--Manually annotated by BRENDA team
Sphingomonas elodeaATCC 31461660765--Manually annotated by BRENDA team
Sphingomonas elodeastrain ATCC 31461671154, 674334--Manually annotated by BRENDA team
Streptococcus equi subsp. zooepidemicusATCC 39920701878Q8GQP9SwissProtManually annotated by BRENDA team
Streptococcus pneumoniae-661546--Manually annotated by BRENDA team
Streptomyces hygroscopicusstrain KCCM 11405, recombinant protein. Enzyme is part of the validamycin A biosynthetic pathway675332--Manually annotated by BRENDA team
Streptomyces hygroscopicus KCCM 11405strain KCCM 11405, recombinant protein. Enzyme is part of the validamycin A biosynthetic pathway675332--Manually annotated by BRENDA team
Streptomyces thermophilusstrain LY03643754--Manually annotated by BRENDA team
Streptomyces thermophilus LY03strain LY03643754--Manually annotated by BRENDA team
Sus scrofawild-type and mutant RN-663369--Manually annotated by BRENDA team
Typha latifolia-643727--Manually annotated by BRENDA team
Xanthomonas axonopodis pv. citri-702480Q8PK83UniProtManually annotated by BRENDA team
Xanthomonas campestris pv. campestris-702480Q4UVD0SwissProtManually annotated by BRENDA team
Zea mays-643748--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
metabolismArabidopsis thaliana-involved in sulfolipid synthesis706224
metabolismMycobacterium tuberculosis H37RvO05576involved in cell envelope precursor synthesis706591

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + alpha-D-glucose 1-phosphatediphosphate + ADP-glucose
show the reaction diagram		Hordeum vulgare subsp. vulgare-13.7% of the activity with UTP643752-643752r
diphosphate + CDP-glucoseCTP + alpha-D-glucose 1-phosphate
show the reaction diagram		Sorghum bicolor-no activity643743---
diphosphate + CDP-glucoseCTP + alpha-D-glucose 1-phosphate
show the reaction diagram		Acanthamoeba castellanii-no activity643745---
diphosphate + CDP-glucoseCTP + alpha-D-glucose 1-phosphate
show the reaction diagram		Homo sapiens, Bos taurus-calf and human liver, poor substrate643748-643748r
diphosphate + CDP-glucoseCTP + alpha-D-glucose 1-phosphate
show the reaction diagram		Homo sapiens-very low activity, wild-type and mutant W218643751-643751r
diphosphate + CDP-glucoseCTP + alpha-D-glucose 1-phosphate
show the reaction diagram		Hordeum vulgare subsp. vulgare-18.5% of the activity with UTP643752-643752r
diphosphate + GDP-glucoseGTP + alpha-D-glucose 1-phosphate
show the reaction diagram		Sorghum bicolor-no activity643743---
diphosphate + GDP-glucoseGTP + alpha-D-glucose 1-phosphate
show the reaction diagram		Acanthamoeba castellanii-no activity643745---
diphosphate + GDP-glucoseGTP + alpha-D-glucose 1-phosphate
show the reaction diagram		Homo sapiens, Bos taurus-calf and human liver, poor substrate643748--r
diphosphate + GDP-glucoseGTP + alpha-D-glucose 1-phosphate
show the reaction diagram		Hordeum vulgare subsp. vulgare-14.3% of the activity with UTP643752-643752r
diphosphate + TDP-glucoseTTP + alpha-D-glucose 1-phosphate
show the reaction diagram		Sphingomonas elodea--674334--?
diphosphate + TDP-glucoseTTP + alpha-D-glucose 1-phosphate
show the reaction diagram		Acanthamoeba castellanii-no activity643745---
diphosphate + TDP-glucoseTTP + alpha-D-glucose 1-phosphate
show the reaction diagram		Homo sapiens-poor substrate643736-643736r
diphosphate + TDP-glucoseTTP + alpha-D-glucose 1-phosphate
show the reaction diagram		Homo sapiens-poor substrate643748-643748r
diphosphate + TDP-glucoseTTP + alpha-D-glucose 1-phosphate
show the reaction diagram		Homo sapiens-poor substrate643751-643751r
diphosphate + TDP-glucoseTTP + alpha-D-glucose 1-phosphate
show the reaction diagram		Bos taurus-poor substrate643736-643736r
diphosphate + TDP-glucoseTTP + alpha-D-glucose 1-phosphate
show the reaction diagram		Bos taurus-poor substrate643748-643748r
diphosphate + TDP-glucoseTTP + alpha-D-glucose 1-phosphate
show the reaction diagram		Oryctolagus cuniculus-poor substrate643736-643736r
diphosphate + TDP-glucoseTTP + alpha-D-glucose 1-phosphate
show the reaction diagram		Oryctolagus cuniculus-poor substrate643748-643748r
diphosphate + TDP-glucoseTTP + alpha-D-glucose 1-phosphate
show the reaction diagram		Sorghum bicolor-poor substrate643743-643743r
diphosphate + TDP-glucoseTTP + alpha-D-glucose 1-phosphate
show the reaction diagram		Homo sapiens, Bos taurus-calf and human liver643748-643748r
diphosphate + TDP-glucoseTTP + alpha-D-glucose 1-phosphate
show the reaction diagram		Homo sapiens-very low activity, wild-type and mutant W218643751-643751r
diphosphate + UDP-galactoseUTP + alpha-D-galactose 1-phosphate
show the reaction diagram		Cucumis meloQ19TV8-676614--r
diphosphate + UDP-glucoseUTP + alpha-D-glucose 1-phosphate
show the reaction diagram		Leishmania majorQ4QDU3-674571--r
diphosphate + UDP-glucoseUTP + alpha-D-glucose 1-phosphate
show the reaction diagram		Cucumis meloQ19TV8-676614--r
diphosphate + UDP-mannoseUTP + D-mannose 1-phosphate
show the reaction diagram		Homo sapiens, Bos taurus-calf and human liver, poor substrate643748--r
diphosphate + UDP-xyloseUTP + D-xylose 1-phosphate
show the reaction diagram		Homo sapiens--643732--r
diphosphate + UDP-xyloseUTP + D-xylose 1-phosphate
show the reaction diagram		Homo sapiens-poor substrate643736, 643748--r
diphosphate + UDP-xyloseUTP + D-xylose 1-phosphate
show the reaction diagram		Bos taurus-poor substrate643736, 643748--r
diphosphate + UDP-xyloseUTP + D-xylose 1-phosphate
show the reaction diagram		Oryctolagus cuniculus-poor substrate643736, 643748--r
diphosphate + UDP-xyloseUTP + D-xylose 1-phosphate
show the reaction diagram		Homo sapiens, Bos taurus, Oryctolagus cuniculus-calf and human liver643748--r
diphosphate + UDP-xyloseUTP + D-xylose 1-phosphate
show the reaction diagram		Bifidobacterium bifidum-reaction at 10% the rate of UDP-glucose or UDP-galactose pyrophosphorolysis642818--r
TTP + alpha-D-glucose 1-phosphateTDP-glucose + diphosphate
show the reaction diagram		Xanthomonas campestris pv. campestrisQ4UVD0-702480--r
TTP + alpha-D-glucose 1-phosphateTDP-glucose + diphosphate
show the reaction diagram		Xanthomonas axonopodis pv. citriQ8PK83-702480--r
UDP-glucose + adenosine 5'-tetraphosphateD-glucose-1-phosphate + adenosyl-5'-pentaphospho-5'-uridine
show the reaction diagram		Saccharomyces cerevisiae--661732--?
UDP-glucose + ATPD-glucose-1-phosphate + adenosyl-5'-tetraphospho-5'-uridine
show the reaction diagram		Saccharomyces cerevisiae--661732--?
UDP-glucose + CTPD-glucose-1-phosphate + cytidinyl-5'-tetraphospho-5'-uridine
show the reaction diagram		Saccharomyces cerevisiae--661732--?
UDP-glucose + GTPD-glucose-1-phosphate + guanosyl-5'-tetraphospho-5'-uridine
show the reaction diagram		Saccharomyces cerevisiae--661732--?
UDP-glucose + guanosine 5'-tetraphosphateD-glucose-1-phosphate + guanosyl-5'-pentaphospho-5'-uridine
show the reaction diagram		Saccharomyces cerevisiae--661732--?
UDP-glucose + tetrapolyphosphateuridine-5'-pentaphosphate + D-glucose-1-phosphate
show the reaction diagram		Saccharomyces cerevisiae--661732--?
UDP-glucose + tripolyphosphateuridine-5'-tetraphosphate + D-glucose-1-phosphate
show the reaction diagram		Saccharomyces cerevisiae--661732--?
UTP + alpha-D-galactose 1-phosphatediphosphate + UDP-galactose
show the reaction diagram		Saccharomyces cerevisiae--643748--r
UTP + alpha-D-galactose 1-phosphatediphosphate + UDP-galactose
show the reaction diagram		Saccharomyces cerevisiae--643749-643749r
UTP + alpha-D-galactose 1-phosphatediphosphate + UDP-galactose
show the reaction diagram		Oryctolagus cuniculus--643748--r
UTP + alpha-D-galactose 1-phosphatediphosphate + UDP-galactose
show the reaction diagram		Bifidobacterium bifidum--642817-642817r
UTP + alpha-D-galactose 1-phosphatediphosphate + UDP-galactose
show the reaction diagram		Mus musculus-no activity643726---
UTP + alpha-D-galactose 1-phosphatediphosphate + UDP-galactose
show the reaction diagram		Cucumis sativus-no activity642819---
UTP + alpha-D-galactose 1-phosphatediphosphate + UDP-galactose
show the reaction diagram		Bos taurus-about 15% of the activity with UDP-glucose643736--r
UTP + alpha-D-galactose 1-phosphatediphosphate + UDP-galactose
show the reaction diagram		Oryctolagus cuniculus-equilibrium dialysis: 8 mol UDP-galactose bound per mol enzyme, 3.5% of the activity with UDP-glucose643736--r
UTP + alpha-D-galactose 1-phosphatediphosphate + UDP-galactose
show the reaction diagram		Homo sapiens, Bos taurus-reverse reaction: calf and human liver643748--r
UTP + alpha-D-galactose 1-phosphatediphosphate + UDP-galactose
show the reaction diagram		Bifidobacterium bifidum-at the same rate as UDP-glucose642818-642818r
UTP + alpha-D-galactose 1-phosphatediphosphate + UDP-galactose
show the reaction diagram		Entamoeba histolytica-reaction at 5% the rate of UDP-glucose synthesis642813-642813r
UTP + alpha-D-galactose 1-phosphatediphosphate + UDP-galactose
show the reaction diagram		Homo sapiens-1-12% of the activity with UDP-glucose643736--r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Salmonella enterica subsp. enterica serovar Typhimurium--643746-643746r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Salmonella enterica subsp. enterica serovar Typhimurium, Gallus gallus--643748-643748r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Gallus gallus--643756-643756r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Escherichia coli--643738-643738r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Escherichia coli--643748-643748r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Homo sapiens--642814-642814r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Homo sapiens--643732-643732r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Homo sapiens--643736-643736r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Homo sapiens, Rattus norvegicus--643748-643748r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Saccharomyces cerevisiae--637084-637084r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Saccharomyces cerevisiae--643748-643748r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Saccharomyces cerevisiae--643749-643749r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Saccharomyces cerevisiae--643750-643750r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Bos taurus--643730-643730r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Bos taurus--643731-643731r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Bos taurus--643736-643736r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Bos taurus, Bombyx mori--643748-643748r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Oryctolagus cuniculus--643733-643733r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Oryctolagus cuniculus--643748-643748r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Neurospora crassa--643725-643725r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Ovis aries, Pisum sativum, Zea mays--643748-643748r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Solanum tuberosum--643116-643116r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Solanum tuberosum--643740-643740r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Solanum tuberosum--643742-643742r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Solanum tuberosum--643744-643744r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Solanum tuberosum--705362--r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Arabidopsis thaliana--643755-643755r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Entamoeba histolytica--642813-642813r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Dictyostelium discoideum--643747-643747r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Dictyostelium discoideum, Canis lupus familiaris--643748-643748r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Felis catus--643734-643734r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Saccharomyces fragilis--637084-637084r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Bifidobacterium bifidum--642817-642817r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Capra hircus--643748-643748r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Streptomyces thermophilus--643754-643754r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Lilium longiflorum--643729-643729r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Leishmania major--674795--?
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Typha latifolia--643727-643727r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Hordeum vulgare subsp. vulgare--643739-643739r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Hordeum vulgare subsp. vulgare--643752-643752r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Columba sp., Acetabularia sp., Beta vulgaris subsp. vulgaris var. altissima--643748-643748r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Hordeum vulgare--702453--?
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Hordeum vulgareQ43772-643753-643753r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Hordeum vulgareQ43772-643757-643757r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Leishmania majorQ4QDU3-674571--r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Cucumis meloQ19TV8-676614--r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Mycobacterium tuberculosis H37RvO05576-706591--r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Salmonella enterica subsp. enterica serovar Typhimurium, Gallus gallus, Escherichia coli, Homo sapiens, Rattus norvegicus, Saccharomyces cerevisiae, Bos taurus, Bombyx mori, Oryctolagus cuniculus, Ovis aries, Pisum sativum, Zea mays, Dictyostelium discoideum, Canis lupus familiaris, Capra hircus, Columba sp., Acetabularia sp., Beta vulgaris subsp. vulgaris var. altissima--643748activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose643748r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Mus musculus-specific for alpha-D-glucose 1-phosphate643726-643726r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Cucumis sativus-specific for alpha-D-glucose 1-phosphate642819-642819r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Homo sapiens-specific for alpha-D-glucose 1-phosphate643751specific for UDP-glucose643751r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Felis catus-the soluble enzyme shows an apparent higher affinity for and a 2fold higher activity with alpha-D-glucose 1-phosphate than UTP643735-643735r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Rattus norvegicus-highly specific for UTP643728-643728r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Homo sapiens-highly specific for UTP643751specific for UDP-glucose643751r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Sorghum bicolor-highly specific for UTP643743specific for UDP-glucose643743r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Acanthamoeba castellanii-highly specific for UTP643745specific for UDP-glucose643745r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Sorghum bicolor-reverse reaction: highly specific for UDP-glucose643743specific for UDP-glucose643743r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Oryza sativa-reverse reaction: highly specific for UDP-glucose643724specific for UDP-glucose643724r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Acanthamoeba castellanii-reverse reaction: highly specific for UDP-glucose643745specific for UDP-glucose643745r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Bifidobacterium bifidum-reaction at the same rate as UDP-galactose642818-642818r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Oryctolagus cuniculus-equilibrium dialysis: 8 mol UDP-glucose bound per mol enzyme643736-643736r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Felis catus-reaction critical to mono- and polysaccharide biosynthesis643735-643735r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Lilium longiflorum-cumulative feedback regulation in vivo, modulates the flow of carbon into pathways leading to cell wall polysaccharide synthesis643729-643729r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Escherichia coli-central reaction in galactose and trehalose metabolism643738--r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Acanthamoeba castellanii-enzyme is induced during amoeba encystment643745-643745r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Saccharomyces cerevisiae-enzyme is essential for cell viability643749-643749-
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Streptomyces thermophilus-involved in exopolysaccharide biosynthesis643754--r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Hordeum vulgareQ43772key enzyme in biosynthesis of sucrose, cellulose, and other saccharides643757-643757r
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Streptomyces hygroscopicus-enzyme is specific for UTP675332--?
UTP + alpha-D-glucose 1-phosphateUDP-glucose + diphosphate
show the reaction diagram		Arabidopsis thalianaP57751-702416--r
UTP + alpha-D-glucose 1-phosphateUDP-glucose + diphosphate
show the reaction diagram		Arabidopsis thaliana-14.2% activity with galactose-1-phosphate706224--?
UTP + alpha-D-glucose 1-phosphateUDP-glucose + diphosphate
show the reaction diagram		Xanthomonas campestris pv. campestrisQ4UVD0in the presence of a divalent metal cation (mainly Mg2+)702480--r
UTP + alpha-D-glucose 1-phosphateUDP-glucose + diphosphate
show the reaction diagram		Xanthomonas axonopodis pv. citriQ8PK83in the presence of a divalent metal cation (mainly Mg2+)702480--r
UTP + D-glucose-1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Hordeum vulgare--663047--?
UTP + D-glucose-1-phosphateUDP-glucose + diphosphate
show the reaction diagram		Sus scrofa--663369--?
UTP + D-glucose-1-phosphateUDP-glucose + diphosphate
show the reaction diagram		Pseudomonas aeruginosa--663272--?
UTP + D-glucose-1-phosphateUDP-glucose + diphosphate
show the reaction diagram		Streptococcus pneumoniae--661546--r
UTP + D-glucose-1-phosphateUDP-glucose + diphosphate
show the reaction diagram		Sphingomonas elodea--660765--?
UTP + glucose-1-phosphateUDP-glucose + diphosphate
show the reaction diagram		Mus musculus--662442--?
monothiodiphosphate + UDP-glucoseUTPbeta,gammaS + alpha-D-glucose 1-phosphate
show the reaction diagram		Saccharomyces cerevisiae-poor substrate, 4.8% of Vmax with diphosphate643750UTPbeta,gammaS is chemically unstable and undergoes hydrolysis to UDPbetaS and phosphate, which renders the otherwise reversible reaction irreversible, t1/2 at 25°C: 0.5 min643750ir
additional information?-Hordeum vulgare subsp. vulgare-substrate specificity643752---
additional information?-Solanum tuberosum-no activity with ADP-glucose643740, 643742---
additional information?-Sorghum bicolor-no activity with ADP-glucose643743---
additional information?-Acanthamoeba castellanii-no activity with ADP-glucose643745---
additional information?-Solanum tuberosum-no activity with ATP, CTP, and GTP643742---
additional information?-Sorghum bicolor-no activity with ATP, CTP, and GTP643743---
additional information?-Acanthamoeba castellanii-no activity with ATP, CTP, and GTP643745---
additional information?-Bifidobacterium bifidum-UDP-glucose and UDP-galactose pyrophosphorolysis activity in Bifidobacterium bifidum are catalyzed by a single enzyme protein642817, 642818---
additional information?-Sorghum bicolor-no activity with TPP643743---
additional information?-Escherichia coli-TTP is a poor substrate643738---
additional information?-Arabidopsis thaliana-regulation643755---
additional information?-Gallus gallus-involved in synthesis of hemagglutinin, sulfated proteoglycans, and glycogen in cartilage643756---
additional information?-Pseudomonas aeruginosa-no utilization of the nucleotide diphosphate sugars UDP-Gal, UDP-GlnA, UDP-Man, UDP-Xyl, ADP-Glc, CDP-Glc and TDP-Glc663272---
additional information?-Saccharomyces cerevisiae-UDP-galactose is not a UMP-donor and ADP not a UMP-acceptor661732---
additional information?-Oryza sativaA3QQQ3isoform Ugp1 is required for callose deposition during pollen mother cell meiosis and bridges the apoplastic unloading pathway and pollen development676438---
additional information?-Leishmania majorQ4QDU3molecular geometry at position 4 of glucose is responsible for substrate specificity. The gamma-phosphate group of UTP is essential for binding and for induction of the open conformation, which then allows entry of glucose 1-phosphate674571---
additional information?-Cucumis meloQ19TV8no substrate: alpha-D-glalctose 1-phosphate676614---

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Arabidopsis thaliana--643755-643755
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Salmonella enterica subsp. enterica serovar Typhimurium, Gallus gallus, Escherichia coli, Homo sapiens, Rattus norvegicus, Saccharomyces cerevisiae, Bos taurus, Bombyx mori, Oryctolagus cuniculus, Ovis aries, Pisum sativum, Zea mays, Dictyostelium discoideum, Canis lupus familiaris, Capra hircus, Columba sp., Acetabularia sp., Beta vulgaris subsp. vulgaris var. altissima--643748activation of glucosyl residues for glycogen synthesis, participates in synthesis of numerous compounds including cell wall polymers in higher plants and microorganisms, starch, trehalose, glycosides, glycolipids, heparin, microbial antigens, lactose, glucuronides, and rhamnose643748
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Felis catus-reaction critical to mono- and polysaccharide biosynthesis643735-643735
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Lilium longiflorum-cumulative feedback regulation in vivo, modulates the flow of carbon into pathways leading to cell wall polysaccharide synthesis643729-643729
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Escherichia coli-central reaction in galactose and trehalose metabolism643738--
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Acanthamoeba castellanii-enzyme is induced during amoeba encystment643745-643745
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Saccharomyces cerevisiae-enzyme is essential for cell viability643749-643749
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Streptomyces thermophilus-involved in exopolysaccharide biosynthesis643754--
UTP + alpha-D-glucose 1-phosphatediphosphate + UDP-glucose
show the reaction diagram		Hordeum vulgareQ43772key enzyme in biosynthesis of sucrose, cellulose, and other saccharides643757-643757
additional information?-Arabidopsis thaliana-regulation643755--
additional information?-Gallus gallus-involved in synthesis of hemagglutinin, sulfated proteoglycans, and glycogen in cartilage643756--
additional information?-Oryza sativaA3QQQ3isoform Ugp1 is required for callose deposition during pollen mother cell meiosis and bridges the apoplastic unloading pathway and pollen development676438--

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
No entries in this field

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
Ca2+Bos taurus-28% as effective as Mg2+ at 1 mM; activation643736
Ca2+Homo sapiens-16% as effective as Mg2+ at 2 mM; activation643736
Ca2+Oryctolagus cuniculus-50% as effective as Mg2+ at 1 mM; activation643736
Ca2+Acanthamoeba castellanii-activation643745
Co2+Rattus norvegicus-activation; no effect on liver enzyme form I, 2fold activation of enzyme form from sublingual gland, inhibition of enzyme form from submandibular gland643728
Co2+Bos taurus-75% activity at 2 mM; activation643736
Co2+Homo sapiens, Oryctolagus cuniculus-47-49% activity at 2 mM; activation643736
Co2+Sorghum bicolor-activation; Co2+ in excess of diphosphate-concentration inhibits643743
Co2+Acanthamoeba castellanii-activation643745
Co2+Acetabularia sp., Beta vulgaris subsp. vulgaris var. altissima, Bombyx mori, Bos taurus, Canis lupus familiaris, Capra hircus, Columba sp., Dictyostelium discoideum, Escherichia coli, Gallus gallus, Homo sapiens, Oryctolagus cuniculus, Ovis aries, Pisum sativum, Rattus norvegicus, Saccharomyces cerevisiae, Salmonella enterica subsp. enterica serovar Typhimurium, Zea mays-about 25% as effective as Mg2+643748
MagnesiumCorynebacterium glutamicumQ6M6R3crystallization data, two magnesium ions coordinate to UDP-glucose. Magnesium is involved in catalytic mechanism677027
Mg2+Homo sapiens-requirement642814, 643736, 643748
Mg2+Bifidobacterium bifidum-requirement642818
Mg2+Solanum tuberosum-requirement643116
Mg2+Mus musculus-6-8fold increase in activity; activation; requirement643726
Mg2+Rattus norvegicus-activation643728
Mg2+Bos taurus-activation; requirement643730
Mg2+Bos taurus-activation643731
Mg2+Homo sapiens--643732
Mg2+Felis catus-activation643734, 643735
Mg2+Bos taurus, Oryctolagus cuniculus-requirement643736, 643748
Mg2+Solanum tuberosum-Mg-diphosphate is the actual substrate; MgUTP is the actual substrate; requirement643740
Mg2+Solanum tuberosum-5 mM; requirement643742
Mg2+Sorghum bicolor-increasing MgSO4 concentrations shift the apparent equilibrium toward UDP-glucose synthesis; Mg-diphosphate is the actual substrate; requirement643743
Mg2+Acanthamoeba castellanii-Mg-diphosphate is the actual substrate; MgUTP is the actual substrate; requirement643745
Mg2+Acetabularia sp., Beta vulgaris subsp. vulgaris var. altissima, Bombyx mori, Canis lupus familiaris, Capra hircus, Columba sp., Dictyostelium discoideum, Escherichia coli, Gallus gallus, Ovis aries, Pisum sativum, Rattus norvegicus, Saccharomyces cerevisiae, Salmonella enterica subsp. enterica serovar Typhimurium, Zea mays-requirement643748
Mg2+Hordeum vulgare subsp. vulgare-requirement643752
Mg2+Streptococcus pneumoniae--661546
Mg2+Saccharomyces cerevisiae--661732
Mg2+Solanum tuberosum--705362
Mg2+Arabidopsis thaliana-absolute requirement for activity706224
Mg2+Mycobacterium tuberculosis H37RvO05576absolutely required706591
Mn2+Mus musculus-6-8fold increase in activity; activation643726
Mn2+Rattus norvegicus-activation643728
Mn2+Bos taurus-maximal activity at 1 mM643736
Mn2+Homo sapiens--643736
Mn2+Oryctolagus cuniculus-maximal activity at 1 mM643736
Mn2+Sorghum bicolor-about 25% as effective as Mg2+; activation; UDP-glucose synthesis: Mn2+ in excess of diphosphate-concentration inhibits643743
Ni2+Mus musculus-highly stimulatory643726
Ni2+Rattus norvegicus-no effect643728
Ni2+Acetabularia sp., Beta vulgaris subsp. vulgaris var. altissima, Bombyx mori, Bos taurus, Canis lupus familiaris, Capra hircus, Columba sp., Dictyostelium discoideum, Escherichia coli, Gallus gallus, Homo sapiens, Oryctolagus cuniculus, Ovis aries, Pisum sativum, Rattus norvegicus, Saccharomyces cerevisiae, Salmonella enterica subsp. enterica serovar Typhimurium, Zea mays-about 25% as effective as Mg2+643748
Mn2+Acetabularia sp., Beta vulgaris subsp. vulgaris var. altissima, Bombyx mori, Bos taurus, Canis lupus familiaris, Capra hircus, Columba sp., Dictyostelium discoideum, Escherichia coli, Gallus gallus, Homo sapiens, Oryctolagus cuniculus, Ovis aries, Pisum sativum, Rattus norvegicus, Saccharomyces cerevisiae, Salmonella enterica subsp. enterica serovar Typhimurium, Zea mays-about 25% as effective as Mg2+; activation643748
additional informationMus musculus-requirement for divalent cations643726
additional informationOryctolagus cuniculus-requirement for divalent cations643736
additional informationSorghum bicolor-requirement for divalent cations643743
additional informationAcanthamoeba castellanii-requirement for divalent cations643745

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
3-phosphoglycerateSolanum tuberosum-no inhibition of UDP-glucose synthesis643742 2D-image
alpha-D-galactose 1-phosphateBifidobacterium bifidum--642818 2D-image
alpha-D-galactose 1-phosphateBos taurus-no inhibition643731 2D-image
alpha-D-galactose 1-phosphateBos taurus--643736 2D-image
alpha-D-galactose 1-phosphateOryctolagus cuniculus-competitive643736 2D-image
alpha-D-galactose 1-phosphateBos taurus-at high concentrations643748 2D-image
alpha-D-galactose 1-phosphateOryctolagus cuniculus--643748 2D-image
alpha-D-glucose 1-phosphateBifidobacterium bifidum-UDP-glucose pyrophosphorolysis642818 2D-image
alpha-D-glucose 1-phosphateBos taurus-product inhibition643730 2D-image
alpha-D-glucose 1-phosphateSolanum tuberosum-product inhibition643740 2D-image
alpha-D-glucose 1-phosphateSorghum bicolor-product inhibition643743 2D-image
Cd2+Rattus norvegicus--643728 2D-image
Co2+Rattus norvegicus-no effect on liver enzyme form I, 2fold activation of enzyme form from sublingual gland, inhibition of enzyme form from submandibular gland643728 2D-image
Co2+Sorghum bicolor-in excess of diphosphate-concentration, activates at lower concentrations643743 2D-image
D-fructose 2,6-bisphosphateSolanum tuberosum-no inhibition of UDP-glucose synthesis643742 2D-image
D-fructose 6-phosphateSolanum tuberosum--643742 2D-image
D-galactosamineBos taurus--643748 2D-image
D-galactose 6-phosphateBifidobacterium bifidum-UDP-glucose pyrophosphorolysis642818 2D-image
diphosphateRattus norvegicus-product inhibition643728, 643748 2D-image
diphosphateAcanthamoeba castellanii-product inhibition643745 2D-image
diphosphateDictyostelium discoideum-possible alternate regulatory mechanism; product inhibition643748 2D-image
diphosphateHordeum vulgare subsp. vulgare-noncompetitive to glucose 1-phosphate and UTP643752 2D-image
diphosphateArabidopsis thaliana-with 0.25 mM 57% activity, with 1 mM 11% activity, with 10 mM 1.5% activity706224 2D-image
iodoacetamideHomo sapiens-wild-type: loss of 56% activity after 30 min at 0.1 mM, mutant C123S is not affected643751 2D-image
Mg-diphosphateBos taurus-product inhibition643730 2D-image
Mg-diphosphateSorghum bicolor-product inhibition643743 2D-image
MgUTPHomo sapiens-wild-type and mutants C123S, H266R, W218, R389H, R422Q, R445H643751 2D-image
MgUTP2-Bos taurus--643730 2D-image
MgUTP2-Homo sapiens--643751 2D-image
Ni2+Rattus norvegicus--643728 2D-image
phosphateSolanum tuberosum--643742 2D-image
phosphateBos taurus--643748 2D-image
phosphateHordeum vulgare subsp. vulgare-noncompetitive to glucose 1-phosphate and UTP643752 2D-image
SucroseBos taurus-54% inhibition at 2%643730 2D-image
TDP-glucoseEscherichia coli--643748 2D-image
TDP-rhamnoseEscherichia coli--643748 2D-image
UDPHomo sapiens--643748 2D-image
UDP-D-mannoseLilium longiflorum--643729 2D-image
UDP-D-mannoseBos taurus--643731 2D-image
UDP-galactoseBifidobacterium bifidum-UDP-glucose pyrophosphorolysis642818 2D-image
UDP-galactoseLilium longiflorum--643729 2D-image
UDP-galactoseBos taurus-14% inhibition at 0.35 mM643731 2D-image
UDP-galactoseHomo sapiens-strong inhibition643732 2D-image
UDP-galactoseBos taurus, Homo sapiens--643736 2D-image
UDP-galactoseOryctolagus cuniculus-competitive643736 2D-image
UDP-galacturonic acidLilium longiflorum--643729 2D-image
UDP-galacturonic acidBos taurus-48% inhibition at 0.43 m643731 2D-image
UDP-glucoseBifidobacterium bifidum-UDP-galactose pyrophosphorolysis or UDP-glucose synthesis642818 2D-image
UDP-glucoseRattus norvegicus-product inhibition643728 2D-image
UDP-glucoseLilium longiflorum--643729 2D-image
UDP-glucoseBos taurus-87% inhibition at 0.08 mM643731 2D-image
UDP-glucoseSorghum bicolor-product inhibition643743 2D-image
UDP-glucoseHordeum vulgare subsp. vulgare-competitive to UTP, noncompetitive to glucose 1-phosphate643752 2D-image
UDP-glucuronic acidLilium longiflorum--643729 2D-image
UDP-glucuronic acidBos taurus-56% inhibition at 0.45 mM643731 2D-image
UDP-mannoseLilium longiflorum--643729 2D-image
UDP-mannoseBos taurus-23% inhibition at 0.45 mM643731 2D-image
UDP-xyloseBifidobacterium bifidum--642818 2D-image
UDP-xyloseLilium longiflorum--643729 2D-image
UDP-xyloseBos taurus-32% inhibition at 0.36 mM643731 2D-image
UTPBifidobacterium bifidum-UDP-glucose pyrophosphorolysis642818 2D-image
UTPBos taurus-MgUTP2-643730 2D-image
UTPSolanum tuberosum-free form643740 2D-image
UTPSorghum bicolor-product inhibition643743 2D-image
UTPAcanthamoeba castellanii-product inhibition643745 2D-image
UTPHordeum vulgare subsp. vulgare-competitive to UDP-glucose; product inhibition643752 2D-image
Mn2+Sorghum bicolor-in excess of diphosphate-concentration, activates at lower concentrations643743 2D-image
additional informationMus musculus-product inhibition pattern643726-
additional informationLilium longiflorum-inhibition kinetics for UDP-sugars643729-
additional informationBos taurus-no effect of PCMB, iodoacetate, 2-mercaptoethanol or DTT643730-
additional informationBos taurus-inhibition kinetics for UDP-sugars; little or no inhibition by alpha-D-mannose 1-phosphate, alpha-D-xylose 1-phosphate or alpha-D-glucuronic acid 1-phosphate643731-
additional informationAcetabularia sp., Beta vulgaris subsp. vulgaris var. altissima, Bombyx mori, Bos taurus, Canis lupus familiaris, Capra hircus, Columba sp., Dictyostelium discoideum, Escherichia coli, Gallus gallus, Homo sapiens, Oryctolagus cuniculus, Ovis aries, Pisum sativum, Rattus norvegicus, Saccharomyces cerevisiae, Salmonella enterica subsp. enterica serovar Typhimurium, Zea mays--643748-
additional informationHordeum vulgare-not inhibitory: Tween-20663047-

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
2-mercaptoethanolBos taurus--643736, 643748 2D-image
2-mercaptoethanolHomo sapiens-requirement643736, 643748 2D-image
2-mercaptoethanolOryctolagus cuniculus-no activation643736 2D-image
2-mercaptoethanolSorghum bicolor--643743 2D-image
2-mercaptoethanolOryctolagus cuniculus--643748 2D-image
3-phosphoglycerateBifidobacterium bifidum-slight activation642818 2D-image
Co2+Rattus norvegicus-no effect on liver enzyme form I, 2fold activation of enzyme form from sublingual gland, inhibition of enzyme form from submandibular gland643728 2D-image
D-erythrose 4-phosphateBifidobacterium bifidum-slight activation642818 2D-image
D-fructose 6-phosphateBifidobacterium bifidum-slight activation642818 2D-image
dithiothreitolHordeum vulgare-increases wild-type activity by about 25%663047 2D-image
SucroseArabidopsis thaliana-activates; inducible by643755 2D-image
Triton X-100Felis catus-activation, only membrane-bound Golgi-enzyme643734, 643735 2D-image
DTTHomo sapiens-requirement643748 2D-image
additional informationSolanum tuberosum-no activation by 3-phosphoglycerate643116, 643740-
additional informationBos taurus-no effect of PCMB, iodoacetate, 2-mercaptoethanol or DTT643730-
additional informationSolanum tuberosum-no activation by 3-phosphoglycerate; no activation by fructose 2,6-bisphosphate, UDPglucose synthesis643742-
additional informationSorghum bicolor-no activation by 3-phosphoglycerate; no activation by fructose 1,6-bisphosphate, fructose 6-phosphate, phosphoenolpyruvate or p-hydroxymandelonitrile-beta-D-glucopyranoside643743-
additional informationBos taurus--643748-
additional informationRattus norvegicus-rat mammary gland enzyme seems to undergo structural changes that lead to a more active form643748-
additional informationArabidopsis thaliana-enzyme expression is inducible by exogenous sucrose, light and cold stress643755-

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.01-alpha-D-glucose 1-phosphateEscherichia coli-pH 7.8, 37°C643738 2D-image
0.030.048alpha-D-glucose 1-phosphateBos taurus-pH 7.5, 30°C643731 2D-image
0.030.048alpha-D-glucose 1-phosphateBos taurus--643736 2D-image
0.030.048alpha-D-glucose 1-phosphateOryctolagus cuniculus-pH 7.8643736 2D-image
0.030.048alpha-D-glucose 1-phosphateSorghum bicolor-pH 8.5, 37°C643743 2D-image
0.04455-alpha-D-glucose 1-phosphateMycobacterium tuberculosis H37RvO05576-706591 2D-image
0.0550.08alpha-D-glucose 1-phosphateBos taurus--643736 2D-image
0.0550.08alpha-D-glucose 1-phosphateOryctolagus cuniculus-pH 7.8643736 2D-image
0.0550.08alpha-D-glucose 1-phosphateSolanum tuberosum-at physiological substrate concentration, pH 8.5, 37°C643742 2D-image
0.06-alpha-D-glucose 1-phosphateXanthomonas campestris pv. campestrisQ4UVD0-702480 2D-image
0.074-alpha-D-glucose 1-phosphateHordeum vulgare subsp. vulgare-pH 8.35,25°C643752 2D-image
0.095-alpha-D-glucose 1-phosphateHomo sapiens--643736 2D-image
0.095-alpha-D-glucose 1-phosphateOryctolagus cuniculus-pH 7.8643736 2D-image
0.140.18alpha-D-glucose 1-phosphateSolanum tuberosum-pH 8.0, 30°C643740 2D-image
0.140.18alpha-D-glucose 1-phosphateAcanthamoeba castellanii--643745 2D-image
0.14-alpha-D-glucose 1-phosphateArabidopsis thaliana-UDP-glucose synthesis702416 2D-image
0.192-alpha-D-glucose 1-phosphateLeishmania majorQ4QDU3pH 7.8, 25°C674571 2D-image
0.24-alpha-D-glucose 1-phosphateCucumis meloQ19TV8pH 7.5, 30°C676614 2D-image
0.24-alpha-D-glucose 1-phosphateArabidopsis thaliana--706224 2D-image
0.36-alpha-D-glucose 1-phosphateArabidopsis thaliana-UDP-glucose synthesis702416 2D-image
0.46-alpha-D-glucose 1-phosphateLilium longiflorum--643729 2D-image
0.681alpha-D-glucose 1-phosphateCucumis sativus-pH 7.2, 30°C642819 2D-image
0.681alpha-D-glucose 1-phosphateSolanum tuberosum-at substrate concentrations above 2 mM, pH 8.5, 37°C643742 2D-image
0.681alpha-D-glucose 1-phosphateAcanthamoeba castellanii-pH 8.0, 37°C643745 2D-image
0.681alpha-D-glucose 1-phosphateHomo sapiens-recombinant wild-type enzyme, pH 8.0, 25°C643751 2D-image
0.83-alpha-D-glucose 1-phosphateSolanum tuberosum-UDP-glucose synthesis705362 2D-image
0.19-D-glucose-1-phosphateStreptococcus pneumoniae--661546 2D-image
0.83-D-glucose-1-phosphatePseudomonas aeruginosa--663272 2D-image
0.027-diphosphateHordeum vulgare-Y192A mutant protein702453 2D-image
0.03-diphosphateArabidopsis thaliana-pyrophosphorolysis702416 2D-image
0.03-diphosphateHordeum vulgare-Ccut-8 mutant protein; K183A mutant protein702453 2D-image
0.034-diphosphateHordeum vulgareQ43772recombinant wild-type, pH 7.6, 25°C643757 2D-image
0.039-diphosphateHordeum vulgare-wild-type protein702453 2D-image
0.04-diphosphateHordeum vulgare-K332A mutant protein702453 2D-image
0.054-diphosphateSorghum bicolor-pH 8.5, 37°C643743 2D-image
0.07-diphosphateArabidopsis thaliana-pyrophosphorolysis702416 2D-image
0.084-diphosphateBos taurus--643736 2D-image
0.084-diphosphateOryctolagus cuniculus-pH 7.8643736 2D-image
0.09-diphosphateHordeum vulgare-Ncut-21 mutant protein702453 2D-image
0.110.13diphosphateSolanum tuberosum-pH 8.0, 30°C643740 2D-image
0.110.13diphosphateSolanum tuberosum-at physiological substrate concentration, pH 8.5, 37°C643742 2D-image
0.115-diphosphatePseudomonas aeruginosa--663272 2D-image
0.17-diphosphateHordeum vulgare subsp. vulgare-pH 8.35, 25°C643752 2D-image
0.18-diphosphateHomo sapiens-non-galactosemic phenotype, pH 7.8, 37°C642814 2D-image
0.2-diphosphateHomo sapiens-galactosemic phenotype, pH 7.8, 37°C642814 2D-image
0.2-diphosphateLeishmania majorQ4QDU3pH 7.8, 25°C674571 2D-image
0.21-diphosphateHomo sapiens--643736 2D-image
0.21-diphosphateOryctolagus cuniculus-pH 7.8643736 2D-image
0.24-diphosphateHordeum vulgare-K405A mutant protein702453 2D-image
0.26-diphosphateStreptococcus pneumoniae--661546 2D-image
0.407-diphosphateHordeum vulgareQ43772recombinant mutant C99S, pH 7.6, 25°C643757 2D-image
0.48-diphosphateXanthomonas campestris pv. campestrisQ4UVD0-702480 2D-image
0.9-diphosphateHordeum vulgare-Ncut-37 mutant protein702453 2D-image
2.4-diphosphateAcanthamoeba castellanii-pH 7.6, 37°C643745 2D-image
4.9-diphosphateHordeum vulgare-K260A mutant protein702453 2D-image
6.4-diphosphateHordeum vulgare-Ccut-32 mutant protein702453 2D-image
9.8-diphosphateHordeum vulgare-Ccut-67 mutant protein702453 2D-image
29.4-diphosphateHordeum vulgare-Delta 1-4 mutant protein702453 2D-image
33.3-diphosphateHordeum vulgare-Ccut-101 mutant protein702453 2D-image
60-diphosphateHordeum vulgare-Delta 1-8 mutant protein702453 2D-image
0.33-Mg-diphosphateCucumis sativus-pH 7.2, 30°C642819 2D-image
0.56-Mg-diphosphateSolanum tuberosum-pyrophosphorolysis705362 2D-image
0.22-Mg-UTPSolanum tuberosum-UDP-glucose synthesis705362 2D-image
0.58-Mg2+Xanthomonas campestris pv. campestrisQ4UVD0pyrophosphorolysis direction702480 2D-image
1.11-Mg2+Xanthomonas campestris pv. campestrisQ4UVD0-702480 2D-image
4.5-tripolyphosphateSaccharomyces cerevisiae-30°C, pH 8.0661732 2D-image
0.35-TTPXanthomonas axonopodis pv. citriQ8PK83-702480 2D-image
0.36-TTPXanthomonas campestris pv. campestrisQ4UVD0-702480 2D-image
2-TTPEscherichia coli-pH 7.8, 37°C643738 2D-image
0.26-UDP-galactoseCucumis meloQ19TV8pH 7.5, 30°C676614 2D-image
0.42-UDP-galactoseOryctolagus cuniculus-pH 7.8643736 2D-image
0.03-UDP-glucoseHordeum vulgare-Ccut-8 mutant protein; K183A mutant protein; K332A mutant protein702453 2D-image
0.031-UDP-glucoseHordeum vulgare-Y192A mutant protein702453 2D-image
0.034-UDP-glucoseHordeum vulgare-wild-type protein702453 2D-image
0.035-UDP-glucoseSaccharomyces cerevisiae-30°C, pH 8.0661732 2D-image
0.04-UDP-glucoseArabidopsis thaliana-pyrophosphorolysis702416 2D-image
0.04-UDP-glucoseHordeum vulgare-K405A mutant protein702453 2D-image
0.050.066UDP-glucoseBos taurus, Homo sapiens--643736 2D-image
0.050.066UDP-glucoseOryctolagus cuniculus-pH 7.8643736 2D-image
0.050.066UDP-glucoseSorghum bicolor--643743 2D-image
0.050.066UDP-glucoseAcanthamoeba castellanii-pH 7.6, 37°C643745 2D-image
0.06-UDP-glucoseArabidopsis thaliana-pyrophosphorolysis702416 2D-image
0.065-UDP-glucoseHordeum vulgareQ43772recombinant wild-type, pH 7.6, 25°C643757 2D-image
0.074-UDP-glucoseHordeum vulgare-K260A mutant protein702453 2D-image
0.101-UDP-glucoseHordeum vulgareQ43772recombinant mutant C99S, pH 7.6, 25°C643757 2D-image
0.105-UDP-glucoseLeishmania majorQ4QDU3pH 7.8, 25°C674571 2D-image
0.11-UDP-glucoseCucumis meloQ19TV8pH 7.5, 30°C676614 2D-image
0.118-UDP-glucoseHordeum vulgare-Ccut-101 mutant protein702453 2D-image
0.120.18UDP-glucoseSolanum tuberosum-pH 8.0, 30°C643740 2D-image
0.120.18UDP-glucoseSolanum tuberosum-at physiological substrate concentrations, pH 8.5, 37°C643742 2D-image
0.14-UDP-glucoseHomo sapiens-non-galactosemic phenotype, pH 7.8, 37°C642814 2D-image
0.18-UDP-glucoseHomo sapiens-galactosemic phenotype, pH 7.8, 37°C642814 2D-image
0.19-UDP-glucoseHordeum vulgare subsp. vulgare-pH 8.35, 25°C643752 2D-image
0.31-UDP-glucoseHordeum vulgare-Ccut-32 mutant protein702453 2D-image
0.362-UDP-glucoseHordeum vulgare-Ccut-67 mutant protein702453 2D-image
0.4-UDP-glucoseStreptococcus pneumoniae--661546 2D-image
0.503-UDP-glucoseHordeum vulgare-Delta 1-8 mutant protein702453 2D-image
0.555-UDP-glucoseHordeum vulgare-Delta 1-4 mutant protein702453 2D-image
0.68-UDP-glucoseSolanum tuberosum-pyrophosphorolysis705362 2D-image
0.99-UDP-glucoseXanthomonas campestris pv. campestrisQ4UVD0-702480 2D-image
1.1-UDP-glucoseHordeum vulgare-Ncut-21 mutant protein702453 2D-image
57-UDP-glucoseHordeum vulgare-Ncut-37 mutant protein702453 2D-image
0.0075-UTPSphingomonas elodea-isoenzyme UgpG, 30°C, pH 7.8660765 2D-image
0.01187-UTPMycobacterium tuberculosis H37RvO05576-706591 2D-image
0.03-UTPSorghum bicolor-pH 8.5, 37°C643743 2D-image
0.031-UTPArabidopsis thaliana--706224 2D-image
0.048-UTPHomo sapiens-liver643736 2D-image
0.048-UTPOryctolagus cuniculus-pH 7.8643736 2D-image
0.048-UTPHomo sapiens-liver643748 2D-image
0.048-UTPOryctolagus cuniculus--643748 2D-image
0.0580.07UTPEscherichia coli-pH 7.8, 37°C643738 2D-image
0.0580.07UTPAcanthamoeba castellanii-pH 8.0, 37°C643745 2D-image
0.07-UTPLeishmania majorQ4QDU3pH 7.8, 25°C674571 2D-image
0.08-UTPArabidopsis thaliana-UDP-glucose synthesis702416 2D-image
0.093-UTPHordeum vulgare subsp. vulgare-pH 8.35, 25°C643752 2D-image
0.10.2UTPBos taurus-pH 7.5, 30°C643731 2D-image
0.10.2UTPSolanum tuberosum-pH 8.0, 30°C643740 2D-image
0.10.2UTPSolanum tuberosum-at physiological substrate concentrations, pH 8.5, 37°C643742 2D-image
0.11-UTPXanthomonas axonopodis pv. citriQ8PK83-702480 2D-image
0.14-UTPLilium longiflorum--643729 2D-image
0.17-UTPArabidopsis thaliana-UDP-glucose synthesis702416 2D-image
0.21-UTPXanthomonas campestris pv. campestrisQ4UVD0-702480 2D-image
0.229-UTPSphingomonas elodea-isoenzyme RmlA, 30°C, pH 7.8660765 2D-image
0.24-UTPStreptococcus pneumoniae--661546 2D-image
0.32-UTPCucumis sativus-pH 7.2, 30°C642819 2D-image
0.53-UTPSolanum tuberosum-at substrate concentrations above 2 mM, pH 8.5, 37°C643742 2D-image
0.56-MgUTPHomo sapiens-recombinant wild-type enzyme, pH 8.0, 25°C643751 2D-image
additional information-additional informationBos taurus-kinetics643730-
additional information-additional informationFelis catus-two-substrate kinetics643735-
additional information-additional informationSolanum tuberosum-kinetics643742-
additional information-additional informationSorghum bicolor-kinetics643743-
additional information-additional informationBos taurus, Canis lupus familiaris, Dictyostelium discoideum, Escherichia coli, Homo sapiens, Oryctolagus cuniculus, Rattus norvegicus, Saccharomyces cerevisiae, Salmonella enterica subsp. enterica serovar Typhimurium-kinetic parameters of various organisms, pH 8.0, overview643748-
additional information-additional informationHordeum vulgare subsp. vulgare-kinetics643752-

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
6.8-TTPXanthomonas campestris pv. campestrisQ4UVD0-702480 2D-image
10-TTPXanthomonas axonopodis pv. citriQ8PK83-702480 2D-image
29.3-UTPXanthomonas axonopodis pv. citriQ8PK83-702480 2D-image
40-UTPXanthomonas campestris pv. campestrisQ4UVD0-702480 2D-image
93.44-UTPMycobacterium tuberculosis H37RvO05576-706591 2D-image
55.03-alpha-D-glucose 1-phosphateMycobacterium tuberculosis H37RvO05576-706591 2D-image
additional information-additional informationTypha latifolia--643727-
additional information-additional informationAcetabularia sp., Beta vulgaris subsp. vulgaris var. altissima, Bombyx mori, Bos taurus, Canis lupus familiaris, Capra hircus, Columba sp., Dictyostelium discoideum, Escherichia coli, Gallus gallus, Homo sapiens, Oryctolagus cuniculus, Ovis aries, Pisum sativum, Rattus norvegicus, Saccharomyces cerevisiae, Salmonella enterica subsp. enterica serovar Typhimurium, Zea mays--643748-

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
1240-alpha-D-glucose 1-phosphateMycobacterium tuberculosis H37RvO05576-7065916756
18.3-TTPXanthomonas campestris pv. campestrisQ4UVD0-70248017549
28.3-TTPXanthomonas axonopodis pv. citriQ8PK83-70248017549
183.3-UTPXanthomonas campestris pv. campestrisQ4UVD0-70248017783
266.7-UTPXanthomonas axonopodis pv. citriQ8PK83-70248017783
7870-UTPMycobacterium tuberculosis H37RvO05576-70659117783

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.135-alpha-D-glucose 1-phosphateBos taurus-pH 8.0, 30°C643730 2D-image
0.213-diphosphateHordeum vulgare subsp. vulgare-versus glucose 1-phosphate, pH 8.35, 25°C643752 2D-image
0.95-diphosphateHordeum vulgare subsp. vulgare-versus UTP, pH 8.35, 25°C643752 2D-image
0.095-MgUTP2-Homo sapiens-mutant H266R, pH 8.0, 25°C643751 2D-image
10.9-phosphateHordeum vulgare subsp. vulgare-versus UTP, pH 8.35, 25°C643752 2D-image
12.2-phosphateHordeum vulgare subsp. vulgare-versus glucose 1-phosphate, pH 8.35, 25°C643752 2D-image
4.8-UDP-D-galactoseLilium longiflorum--643729 2D-image
4.8-UDP-D-galactoseBos taurus-pH 7.5, 30°C643731 2D-image
0.21-UDP-D-galacturonic acidBos taurus-pH 7.5, 30°C643731 2D-image
0.93-UDP-D-galacturonic acidLilium longiflorum--643729 2D-image
0.005-UDP-D-glucoseBos taurus-pH 7.5, 30°C643731 2D-image
0.05-UDP-D-glucoseSorghum bicolor-pH 8.5, 37°C643743 2D-image
0.13-UDP-D-glucoseLilium longiflorum--643729 2D-image
0.15-UDP-D-glucuronic acidBos taurus-pH 7.5, 30°C643731 2D-image
0.75-UDP-D-glucuronic acidLilium longiflorum--643729 2D-image
9.6-UDP-D-mannoseLilium longiflorum--643729 2D-image
9.6-UDP-D-mannoseBos taurus-pH 7.5, 30°C643731 2D-image
0.24-UDP-D-xyloseBos taurus-pH 7.5, 30°C643731 2D-image
1.6-UDP-D-xyloseLilium longiflorum--643729 2D-image
0.015-UDP-glucoseHordeum vulgare subsp. vulgare-versus glucose 1-phosphate, pH 8.35, 25°C643752 2D-image
0.12-UDP-glucoseHordeum vulgare subsp. vulgare-versus UTP, pH 8.35, 25°C643752 2D-image
0.104-UTPSorghum bicolor-pH 8.5, 37°C643743 2D-image
0.17-UTPHordeum vulgare subsp. vulgare-versus UDP-glucose, pH 8.35, 25°C643752 2D-image
0.42-MgUTP2-Bos taurus-pH 8.0, 30°C643730 2D-image
additional information-additional informationLilium longiflorum-simple additive inhibition of inhibitors at low individual concentrations and low substrate concentrations643729-
additional information-additional informationBos taurus-inhibition kinetics643730-
additional information-additional informationBos taurus, Homo sapiens, Oryctolagus cuniculus--643736-
additional information-additional informationAcetabularia sp., Beta vulgaris subsp. vulgaris var. altissima, Bombyx mori, Bos taurus, Canis lupus familiaris, Capra hircus, Columba sp., Dictyostelium discoideum, Escherichia coli, Gallus gallus, Homo sapiens, Oryctolagus cuniculus, Ovis aries, Pisum sativum, Rattus norvegicus, Saccharomyces cerevisiae, Salmonella enterica subsp. enterica serovar Typhimurium, Zea mays-inhibition constants of various organisms643748-

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
0-Hordeum vulgare-Delta-NB mutant protein702453
0.02-Hordeum vulgare-Ncut-37 mutant protein702453
0.1005-Mycobacterium tuberculosis H37RvO05576-706591
0.110.22Streptomyces thermophilus-activity in cell extracts of cells grown on different sugar sources643754
0.21-Hordeum vulgare-Ccut-101 mutant protein702453
0.27-Hordeum vulgare-Ccut-32 mutant protein702453
0.42-Hordeum vulgare-K260A mutant protein702453
2.1-Hordeum vulgare-Delta 1-4 mutant protein702453
2.15-Felis catus-partially purified Golgi apparatus isozyme643734
3.1-Hordeum vulgare-Ccut-67 mutant protein702453
3.7-Leishmania major-mutant H191N, pH 7.8, 25°C674795
3.9-Cucumis sativus-partially purified enzyme642819
4.76-Escherichia coli-partially purified recombinant enzyme643738
5.4-Hordeum vulgare-Delta 1-8 mutant protein702453
7.8-Leishmania major-mutant K95A, pH 7.8, 25°C674795
8.95-Bos taurus-partially purified enzyme643731
12.8-Hordeum vulgare-Ncut-21 mutant protein702453
15.2-Bifidobacterium bifidum-purified enzyme642817, 642818
15.8-Acanthamoeba castellanii-purified enzyme643745
16-Bos taurus-purified enzyme643730
200-Bos taurus, Homo sapiens--643736
200-Oryctolagus cuniculus-purified enzyme643736
240-Leishmania major-mutant L281D, pH 7.8, 25°C674795
251-Solanum tuberosum-pyrophosphorolysis705362
340-Arabidopsis thaliana-UDP-glucose synthesis702416
385-Arabidopsis thaliana-UDP-glucose synthesis702416
449-Solanum tuberosum-UDP-glucose synthesis705362
495-Oryctolagus cuniculus-purified enzyme643733
510-Arabidopsis thaliana-pyrophosphorolysis702416
591-Hordeum vulgare-Y192A mutant protein702453
715-Arabidopsis thaliana-pyrophosphorolysis702416
752-Hordeum vulgare-K183A mutant protein702453
764-Hordeum vulgare-K405A mutant protein702453
853-Hordeum vulgare-K332A mutant protein702453
899-Solanum tuberosum-purified enzyme643740
1074-Hordeum vulgare-wild-type protein702453
1099-Solanum tuberosum-purified enzyme643742
1200-Sorghum bicolor-purified enzyme643743
1477-Leishmania major-wild-type, pH 7.8, 25°C674795
1547-Hordeum vulgare-Ccut-8 mutant protein702453
additional information-Homo sapiens--642814
additional information-Typha latifolia--643727
additional information-Felis catus--643734
additional information-Solanum tuberosum-activity in different tissues643742
additional information-Saccharomyces cerevisiae-activity of several mutant strains in vivo643749
additional information-Sphingomonas elodea-Vmax isoenzyme RmlA 37.05, 30°C, pH 7.8; Vmax isoenzyme UgpG 1.28, 30°C, pH 7.8660765
additional information-Mus musculus-2-3 fold higher activity in transgenic tibialis anterior, extensor digitorum longus and gastrocnemius muscles than in wild type. Very little increase in activity in transgenic soleus muscle. Transgenes PPL-1 and PPL-2662442
additional information-Arabidopsis thaliana-the UGPase protein content and enzyme activity in the Pi-deficient mutant is about twice as high as in wild type and Pi-excess mutants, regardless of the light/dark conditions662716
additional information-Hordeum vulgare-similar activities of the wild-type UGPase in Tris, Hepes or Mops buffers. Half of the activity in 100 mM sodium phosphate. Compared with the wild-type UGPase the mutants KK127-128LL, C99S and LIV135-137NIN display reduced activities in all buffers.663047
additional information-Sus scrofa-activity of UDPGP is significantly up-regulated in muscle tissues from RN- mutants663369

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
5.57Bifidobacterium bifidum-broad642818
67.5Cucumis sativus-broad642819
6.57.5Hordeum vulgare subsp. vulgare--643739
6.58Oryctolagus cuniculus, Saccharomyces cerevisiae--643748
710.5Oryctolagus cuniculus-broad643736
79Sorghum bicolor-pyrophosphorolysis643743
79.5Bos taurus-broad643736
7.38.6Homo sapiens-broad642814
7.57.6Acanthamoeba castellanii-pyrophosphorolysis643745
7.59Escherichia coli, Pisum sativum, Rattus norvegicus--643748
7.5-Bos taurus-assay at643731
7.5-Streptomyces thermophilus-assay at643754
7.69.2Homo sapiens-broad643736, 643748
7.6-Gallus gallus-assay at643756
7.6-Hordeum vulgareQ43772assay at643757
7.8-Escherichia coli-assay at643738
7.8-Dictyostelium discoideum, Escherichia coli--643748
88.6Felis catus-Golgi membrane and cytosolic isozyme643735
89Sorghum bicolor-UDP-glucose synthesis643743
89Acanthamoeba castellanii-UDP-glucose synthesis643745
89Bos taurus, Homo sapiens-bovine mammary gland, human erythrocytes643748
8-Bos taurus-assay at643730
8-Oryctolagus cuniculus--643733
8-Mycobacterium tuberculosis H37RvO05576-706591
8.35-Hordeum vulgare subsp. vulgare-assay at643752
8.5-Solanum tuberosum--643742
8.5-Bos taurus--643748
additional information-Oryza sativa--643724
additional information-Mus musculus-active over a wide range643726
additional information-Oryctolagus cuniculus--643733
additional information-Felis catus--643734
additional information-Escherichia coli, Oryctolagus cuniculus--643748
additional information-Salmonella enterica subsp. enterica serovar Typhimurium-isozymes with different pH-optima643748

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
79Sorghum bicolor-about 75% of maximal activity at pH 7.0 and maximal activity at pH 9.0643743
7.29.8Acanthamoeba castellanii-about half-maximal activity at pH 7.2 and about 90% of maximal activity at pH 9.8643745
7.59Solanum tuberosum--643742
additional information-Mus musculus-active over a wide range of pH for both forward and reverse reaction643726

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
21-Arabidopsis thaliana-assay at643755
23-Bos taurus-assay at643730
25-Hordeum vulgare subsp. vulgare-assay at643752
25-Gallus gallus-assay at643756
25-Hordeum vulgareQ43772assay at643757
30-Bos taurus-assay at643730, 643731
30-Solanum tuberosum-assay at643740
37-Homo sapiens-assay at642814
37-Bifidobacterium bifidum-assay at642817, 642818
37-Escherichia coli-assay at643738
37-Solanum tuberosum-assay at643742
37-Sorghum bicolor-assay at643743
37-Acanthamoeba castellanii-assay at643745
37-Streptomyces thermophilus-assay at643754
38-Felis catus-assay at643735

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
2560Hordeum vulgare subsp. vulgare--643739
3545Pseudomonas aeruginosa-mutant pHC3007663272

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
3.9-Felis catus-Golgi isozyme643734
4.2-Felis catus-cytosolic isozyme643734
4.64.7Oryza sativa--643724
5.4-Solanum tuberosum-IEF-PAGE705362
5.45-Xanthomonas campestris pv. campestrisQ4UVD0calculated702480
5.47-Hordeum vulgare-K183A mutant protein; K332A mutant protein; K405A mutant protein702453
5.54-Hordeum vulgare-Ncut-21 mutant protein702453
5.55-Hordeum vulgare-Delta 1-8 mutant protein; Delta-NB mutant protein; wild-type protein; Y192A mutant protein702453
5.58-Hordeum vulgare-Ccut-101 mutant protein702453
5.63-Hordeum vulgare-Delta 1-4 mutant protein702453
5.64-Xanthomonas axonopodis pv. citriQ8PK83calculated702480
5.69-Hordeum vulgare-Ccut-67 mutant protein702453
5.7-Hordeum vulgare-Ncut-37 mutant protein702453
5.79-Hordeum vulgare-Ccut-32 mutant protein702453
5.8-Hordeum vulgare-Ccut-8 mutant protein702453
6.1-Arabidopsis thaliana-calculated for recombinant protein702416
6.14-Arabidopsis thaliana-calculated for recombinant protein702416
6.25-Oryctolagus cuniculus--643733

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
adipose tissueRattus norvegicus--643748Manually annotated by BRENDA team
brainHomo sapiens--643748Manually annotated by BRENDA team
cartilageGallus gallus-hypertrophic and nonhypertrophic643756Manually annotated by BRENDA team
cauline leafArabidopsis thaliana-; 706183Manually annotated by BRENDA team
cell cultureGallus gallus-of hypertrophic and nonhypertrophic cells and chondrocytes643756Manually annotated by BRENDA team
cell suspension cultureSolanum tuberosum--643742Manually annotated by BRENDA team
chondrocyteGallus gallus-hypertrophic and nonhypertrophic643756Manually annotated by BRENDA team
cystAcanthamoeba castellanii-; enzyme activity increases during encystment643745Manually annotated by BRENDA team
Ehrlich ascites carcinoma cellMus musculus--643726Manually annotated by BRENDA team
embryoHordeum vulgare--643753Manually annotated by BRENDA team
embryoGallus gallus--643756Manually annotated by BRENDA team
endospermHordeum vulgare--643753Manually annotated by BRENDA team
erythrocyteHomo sapiens--643748Manually annotated by BRENDA team
extensor digitorum longusMus musculus--662442Manually annotated by BRENDA team
floretOryza sativaA3QQQ3highest expression of enzyme676438Manually annotated by BRENDA team
flowerPopulus tremula x Populus tremuloides-male flower, isoform UGP1 is the predominant transcript673839Manually annotated by BRENDA team
flowerArabidopsis thaliana-; 706183Manually annotated by BRENDA team
fruitCucumis meloQ19TV8expression throughout fruit development676614Manually annotated by BRENDA team
fruit peduncleCucumis sativus--642819Manually annotated by BRENDA team
gastrocnemius muscleMus musculus--662442Manually annotated by BRENDA team
heartCanis lupus familiaris--643748Manually annotated by BRENDA team
leafSolanum tuberosum-low level and activity643742Manually annotated by BRENDA team
leafHordeum vulgare--643753, 643757Manually annotated by BRENDA team
leafPopulus tremula x Populus tremuloides-isoform UGP1 is the predominant transcript. Isoform UGP1, but not UGP2 is upregulated by light and short-term sucrose feeding. Isoform UGP2 is transiently upregulated by cold treatment673839Manually annotated by BRENDA team
leafArabidopsis thaliana--706183Manually annotated by BRENDA team
liverRattus norvegicus-2 forms643728Manually annotated by BRENDA team
liverBos taurus--643731, 643736, 643748Manually annotated by BRENDA team
liverHomo sapiens--643732, 643736, 643748, 643751Manually annotated by BRENDA team
liverFelis catus-cytosolic and Golgi apparatus isozymes643734, 643735Manually annotated by BRENDA team
liverOryctolagus cuniculus--643736, 643748Manually annotated by BRENDA team
liverCapra hircus, Ovis aries, Rattus norvegicus--643748Manually annotated by BRENDA team
mammary glandBos taurus--643730, 643748Manually annotated by BRENDA team
muscleOryctolagus cuniculus--643733, 643748Manually annotated by BRENDA team
muscleRattus norvegicus--643748Manually annotated by BRENDA team
myceliumNeurospora crassa--643725Manually annotated by BRENDA team
ovaryBombyx mori--643748Manually annotated by BRENDA team
pollenTypha latifolia--643727Manually annotated by BRENDA team
pollenLilium longiflorum-non-germinated643729Manually annotated by BRENDA team
pollenOryza sativaA3QQQ3high expression during anther development676438Manually annotated by BRENDA team
rootSolanum tuberosum-low level and activity643742Manually annotated by BRENDA team
rootPopulus tremula x Populus tremuloides-root xylem, isoform UGP1 is the predominant transcript, no upregulation of isoform UGP2 by cold treatment673839Manually annotated by BRENDA team
rootArabidopsis thaliana-; 700488, 706183Manually annotated by BRENDA team
salivary glandRattus norvegicus-1 form643728Manually annotated by BRENDA team
scutellumOryza sativa--643724Manually annotated by BRENDA team
seedHordeum vulgare--643753Manually annotated by BRENDA team
seedlingOryza sativa-predominantly scutellum643724Manually annotated by BRENDA team
seedlingHordeum vulgare subsp. vulgare-germinated, malt643739, 643752Manually annotated by BRENDA team
seedlingSorghum bicolor-etiolated643743Manually annotated by BRENDA team
seedlingArabidopsis thalianaP57751, Q9M9P3; 700488Manually annotated by BRENDA team
siliqueArabidopsis thaliana-; 706183Manually annotated by BRENDA team
skeletal muscleOryctolagus cuniculus--643733Manually annotated by BRENDA team
skin fibroblastHomo sapiens-from normal and galactosemic individuals642814Manually annotated by BRENDA team
soleusMus musculus--662442Manually annotated by BRENDA team
stemSolanum tuberosum--643742Manually annotated by BRENDA team
stemPopulus tremula x Populus tremuloides-isoform UGP2 is upregulated by cold treatment673839Manually annotated by BRENDA team
stemArabidopsis thaliana--706183Manually annotated by BRENDA team
sternumGallus gallus--643756Manually annotated by BRENDA team
stolonSolanum tuberosum--643742Manually annotated by BRENDA team
sublingual glandRattus norvegicus--643728Manually annotated by BRENDA team
submandibular glandRattus norvegicus--643728Manually annotated by BRENDA team
tibialis anteriorMus musculus--662442Manually annotated by BRENDA team
trophozoiteAcanthamoeba castellanii--643745Manually annotated by BRENDA team
tuberSolanum tuberosum-immature643116, 643744Manually annotated by BRENDA team
tuberSolanum tuberosum--643740, 643742Manually annotated by BRENDA team
mammary glandAcetabularia sp., Beta vulgaris subsp. vulgaris var. altissima, Bombyx mori, Canis lupus familiaris, Capra hircus, Columba sp., Dictyostelium discoideum, Escherichia coli, Gallus gallus, Homo sapiens, Oryctolagus cuniculus, Ovis aries, Rattus norvegicus, Salmonella enterica subsp. enterica serovar Typhimurium--643748Manually annotated by BRENDA team
additional informationSolanum tuberosum-tissue distribution643742Manually annotated by BRENDA team
additional informationAcetabularia sp., Beta vulgaris subsp. vulgaris var. altissima, Bombyx mori, Bos taurus, Canis lupus familiaris, Capra hircus, Columba sp., Dictyostelium discoideum, Escherichia coli, Gallus gallus, Homo sapiens, Oryctolagus cuniculus, Ovis aries, Pisum sativum, Rattus norvegicus, Saccharomyces cerevisiae, Salmonella enterica subsp. enterica serovar Typhimurium, Zea mays-most abundant in tissues which display active polysaccharide synthesis; tissue distribution643748Manually annotated by BRENDA team
additional informationGallus gallus-enzyme expression is upregulated in hypertrophic chondrocytes compared to nonhypertrophic chondrocytes, 6fold higher mRNA level643756Manually annotated by BRENDA team
additional informationPopulus tremula x Populus tremuloides-apart from root xylem, leaf and male flowers, isoforms UGP1 and UGP2 are expressed in similar amounts. Enzyme activity and protein correlate in all tissues and conditions. UGP isoforms generally have lower expressions than sucrose synthases; enzyme activity and protein correlate in all tissues and conditions. UGP isoforms generally have lower expressions than sucrose synthases673839Manually annotated by BRENDA team
additional informationOryza sativaA3QQQ3both isoforms Ugp1 and Ugp2 are ubiquitously expressed throughout rice development, with Ugp1 at much higher levels than Ugp2676348Manually annotated by BRENDA team

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
amyloplastOryza sativa--9501643724Manually annotated by BRENDA team
amyloplastPisum sativum, Zea mays--9501643748Manually annotated by BRENDA team
cell wallMycobacterium tuberculosis H37RvO05576-5618706591Manually annotated by BRENDA team
chloroplastAcetabularia sp., Beta vulgaris subsp. vulgaris var. altissima, Pisum sativum, Zea mays--9507643748Manually annotated by BRENDA team
chloroplastArabidopsis thaliana--9507706224Manually annotated by BRENDA team
cytosolOryza sativa--5829643724Manually annotated by BRENDA team
cytosolRattus norvegicus--5829643728Manually annotated by BRENDA team
cytosolFelis catus--5829643734, 643735Manually annotated by BRENDA team
cytosolBeta vulgaris subsp. vulgaris var. altissima, Bombyx mori, Bos taurus, Canis lupus familiaris, Capra hircus, Columba sp.-predominantly, plant or animal cells5829643748Manually annotated by BRENDA team
cytosolEscherichia coli--5829643748Manually annotated by BRENDA team
cytosolGallus gallus, Oryctolagus cuniculus, Ovis aries, Pisum sativum, Rattus norvegicus, Zea mays-predominantly, plant or animal cells5829643748Manually annotated by BRENDA team
Golgi apparatusFelis catus--5794643734, 643735Manually annotated by BRENDA team
Golgi membraneOryza sativa--139643724Manually annotated by BRENDA team
Golgi membraneFelis catus--139643735Manually annotated by BRENDA team
membraneHordeum vulgare--16020643753Manually annotated by BRENDA team
nucleusRattus norvegicus--5634643728Manually annotated by BRENDA team

PDBSCOPCATHORGANISM
2icx, downloadSCOP (2icx)CATH (2icx)Arabidopsis thaliana
2icy, downloadSCOP (2icy)CATH (2icy)Arabidopsis thaliana
2q4j, downloadSCOP (2q4j)CATH (2q4j)Arabidopsis thaliana
2pa4, downloadSCOP (2pa4)CATH (2pa4)Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
2e3d, downloadSCOP (2e3d)CATH (2e3d)Escherichia coli (strain K12)
3r2w, downloadSCOP (3r2w)CATH (3r2w)Homo sapiens
3r3i, downloadSCOP (3r3i)CATH (3r3i)Homo sapiens
2oef, downloadSCOP (2oef)CATH (2oef)Leishmania major
2oeg, downloadSCOP (2oeg)CATH (2oeg)Leishmania major
2i5k, downloadSCOP (2i5k)CATH (2i5k)Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
2ux8, downloadSCOP (2ux8)CATH (2ux8)Sphingomonas elodea
3gue, downloadSCOP (3gue)CATH (3gue)Trypanosoma brucei brucei (strain 927/4 GUTat10.1)

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
31500-Sphingomonas elodea-SDS-PAGE660765
45000-Entamoeba histolytica-gel filtration642813
47000-Solanum tuberosum-gel filtration643740
51000-Hordeum vulgare subsp. vulgare-gel filtration643739
51780-Solanum tuberosum-calculated from nucleotide sequence643744
53000-Typha latifolia-gel filtration643727
53000-Solanum tuberosum-gel filtration643742
54000-Oryza sativa-gel filtration643724
59000-Leishmania majorQ4QDU3gel filtration674571
120000-Sphingomonas elodea-gel filtration674334
130000140000Sphingomonas elodea-gel filtration, dynamic light scattering671154
130000-Escherichia coli-gel filtration643738
130000-Pseudomonas aeruginosa-gel filtration663272
200000-Bifidobacterium bifidum-gel filtration642818
270000-Felis catus-Golgi isozyme, gel filtration643734
300000-Rattus norvegicus--643728
360000-Felis catus-cytosolic isozyme, gel filtration643734
390000-Dictyostelium discoideum-two, non-interconvertible, forms: the second one is lighter and more labile643748
400000-Oryctolagus cuniculus-sucrose density gradient centrifugation643736
430000-Oryctolagus cuniculus-gel filtration643733
450000-Homo sapiens-sucrose density gradient centrifugation643732, 643736
450000-Homo sapiens--643748
450000-Rattus norvegicus-mammary gland643748
480000-Bos taurus-sucrose density gradient centrifugation643736
480000-Bos taurus-multimers of this 480000 MW species exist, ultracentrifugation studies; sucrose density gradient centrifugation643748
500000-Bos taurus-sucrose density gradient centrifugation643730
537000-Neurospora crassa-non-denaturing PAGE643725
580000-Neurospora crassa-sucrose density gradient sedimentation643725
1600000-Neurospora crassa-gel filtration643725
5000000-Mus musculus-approximately643726
additional information-Hordeum vulgareQ43772mixture of monomers, dimers and higher-order polymers, native PAGE and gel filtration, the monomer is the active form643757

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
?Hordeum vulgare-x * 51600, about, embryo, amino acid sequence determination643753
?Gallus gallus-x * 57000, about DNA sequence determination643756
?Sphingomonas elodea-x * 32000, MALDI-TOF of selenomethionine derivative671154
?Solanum tuberosum-x * 53000, SDS-PAGE705362
homodimerXanthomonas campestris pv. campestrisQ4UVD02 * 32000, SDS-PAGE, oligomerization detected after crosslinking702480
monomerOryza sativa-1 * 54000, SDS-PAGE643724
monomerTypha latifolia-1 * 55000, SDS-PAGE643727
monomerHordeum vulgare subsp. vulgare-1 * 54000, SDS-PAGE643739
monomerSolanum tuberosum-1 * 53000, SDS-PAGE643740, 643742
monomerSolanum tuberosum-1 * 51783, calculated from nucleotide sequence643744
monomerLeishmania majorQ4QDU31 * 54500, calculated and SDS-PAGE674571
monomerArabidopsis thaliana-53000, native PAGE and SDS-PAGE, calculated without His-tag: 51600 Da; 53000, native PAGE and SDS-PAGE, calculated without His-tag: 51700 Da702416
octamerHomo sapiens-8 * 56000, SDS-PAGE643732, 643736
octamerOryctolagus cuniculus-8 * 53000, SDS-PAGE643733
octamerBos taurus-8 * 60000, SDS-PAGE643736, 643748
octamerOryctolagus cuniculus-8 * 50000, SDS-PAGE, stacked tetrameric configuration643736
octamerHomo sapiens, Oryctolagus cuniculus--643748
octamerSaccharomyces cerevisiae-crystallization data675416
oligomerHordeum vulgare-SDS-PAGE. Incubation of wild-type UGPase with phosphate or Tris buffers promote oligomerization, whereas Mops and Hepes completely dissociate the oligomers to monomers. Similar buffer effects for mutants KK127-128LL and C99S, small buffer effects for mutant LIV135-137NIN663047
oligomerHordeum vulgare-x * 52000-53000, SDS-PAGE702453
tetramerBifidobacterium bifidum-4 * 51000, SDS-PAGE642818
tetramerEscherichia coli-4 * 38000, SDS-PAGE643738
tetramerPseudomonas aeruginosa-4 * 30943, SDS-PAGE663272
tetramerSphingomonas elodea-crystallization data674334
tetramerEscherichia coliP0AEP3crystallization data677032
monomerMycobacterium tuberculosis H37RvO05576monomer and different oligomers, gel filtration, x * 36000, SDS-PAGE, x * 35827.0, MALDI-TOF (calculated: 35938.8)706591
additional informationNeurospora crassa-SDS-PAGE, 4 polypeptides of different MW643725
additional informationSolanum tuberosum-multimeric forms may exist643742

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
glycoproteinHordeum vulgare-3 putative N-glycosylation sites, possible role in intracellular targeting of the enzyme643753

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
uncomplexed enzyme and in presence of UTP and UDP-glucose. Structures show a carboxy-terminal beta-helix domain in a unique orientation. The nucleotide binding loop and the carboxy-terminal domain, including the possible catalytically important K360, move in and out of the active site in a concerted fashionArabidopsis thaliana-675432
from ammonium sulfate precipitateBos taurus-643736, 643748
from ammonium sulfate precipitateCapra hircus-643748
in complex with both Mg2+ and UDP-glucose. Residues involved in anchoring the ligand to the active site include the polypeptide chain backbone atoms of Ala20, Gly21, Gly117, Gly180, and Ala214, and the side chains of Glu36, Gln112, Asp143, Glu201, and Lys202. Two magnesium ions are coordinated to the UDP-glucose. An alpha- and a beta-phosphoryl oxygen, three waters, and the side chain of Asp142 ligate the first Mg2+ ion, whereas the second ion is coordinated by an alpha-phosphoryl oxygen and five waters; in presence of both magnesium and UDP-glucose. Residues anchoring the ligand to the acitve site include polypepetide backbone atoms of A20, G21, G117, G180 and A214 and side chain residues of E36, Q112, D143, E201, and K202. Two magnesium ions coordinate to UDP-glucoseCorynebacterium glutamicumQ6M6R3677027
protein is a tetramer with 222 point group symmetry. Each subunit is dominated by an eight-stranded mixed beta-sheet with two additional layers of beta-sheets and ten alpha-helices. Q109 and D137 anchor the uracil ring and the ribose of UDP-glucose to the protein.The beta-phosphoryl group of the product lies close to the epsilon-nitrogen of K202, the carboxylate group of E201 can bridge the 2’- and 3’-hydroxyl groups of the glucosyl moiety; to 1.9 A resolution. Modeling of UDP-glucose into the active site. The side chains of Gln109 and Asp137, respectively, serve to anchor the uracil ring and the ribose of UDP-glucose to the protein. The beta-phosphoryl group of the product is predicted to lie within hydrogen bonding distance to the eosilon-nitrogen of Lys202 whereas the carboxylate group of Glu201 is predicted to bridge the 2'- and 3'-hydroxyl groups of the glucosyl moietyEscherichia coliP0AEP3677032
-Helicobacter pylori-660635
uncomplexed apo-protein with open conformation and in complex with UDP-glucose and closed conformation. the central catalyitc domain resembles a Rossman fold and contains key residues. The C-terminal domain forms a left-handed parallel beta-helixLeishmania major-674795
from ammonium sulfate precipitateOryctolagus cuniculus-643736, 643748
from ammonium sulfate precipitateOvis aries-643748
both in solution and crystal, enzyme forms homooctamers. Association of octamers is mediated by left-handed helices in the C-terminal domains forming a toroidal solenoid structure. The catalytic domains do not directly contact each other, consistent with simple Michaelis-Menten kineticsSaccharomyces cerevisiae-675416
detailed comparison between enzyme and thymidylyltransferases; native and seleno-methionine-derivatized proteins, in complex with glucose 1-phosphate, to 2.65 A resolution. Detailed comparison with thymidylyltransferasesSphingomonas elodea-674334
in complex with glucose 1-phosphate, data from an osmium derivative and a selenomethiomine derivativeSphingomonas elodea-671154

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
3-Bos taurus-4°C, 80% loss of activity immediately643730
4-Bos taurus-4°C, t1/2: 2 days643730
5-Bos taurus-86 days, 4°C, 70% loss of activity643730
5.8-Oryctolagus cuniculus-stable643733
68.5Hordeum vulgare subsp. vulgare-47 h, 25°C, 0.1 mg/ml bovine serum albumin, 5% loss of activity. Without bovine serum albumin: 80% or 60% loss of activity within 30 h at pH 6 or pH 6.5, respectively, t1/2: 30 h at pH 7.5 and pH 8.5643739
6.57.5Bos taurus-86 days, 4°C, stable643730
7.59Bos taurus-30 min, stable at 50°C, in 0.25 M potassium phosphate buffer643730
7.8-Oryctolagus cuniculus-rapid inactivation above643733

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
37-Hordeum vulgare subsp. vulgare-30 h, stable at pH 8.0 with 0.1 mg/ml bovine serum albumin, without bovine serum albumin: t1/2: 7 h643739
37-Acanthamoeba castellanii-30 min, stable643745
40-Bifidobacterium bifidum-15 min, 10% loss of activity642817
47-Bifidobacterium bifidum-t1/2: 10 min642817
50-Homo sapiens-15 min, about 10% or 25% loss of activity of enzymes from normal or galactosemic individuals, respectively642814
50-Bos taurus-30 min, stable in 0.25 M potassium phosphate buffer, pH 7.5-9.0643730
50-Homo sapiens-15 min, about 15%, 30% or 25% loss of activity with UDP-glucose, UDP-galactose or UDP-xylose as substrate, respectively643732
50-Mycobacterium tuberculosis H37RvO0557610 min treatment: about 15% activity706591
52-Bos taurus-30 min, 70% loss of activity, 0.25 M potassium phosphate buffer, pH 7.5-9.0643730
56-Homo sapiens-15 min: inactivation; t1/2: 4 min642814
56-Cucumis sativus-2.5 min: 80% loss of activity in crude desalted extracts642819
56-Homo sapiens-15 min: about 65%, 75% or 95% loss of activity with UDP-glucose, UDP-galactose or UDP-xylose as substrate, respectively643732
60-Bifidobacterium bifidum-10 min, inactivation642817
60-Bos taurus-5 min, inactivation643730
additional information-Salmonella enterica subsp. enterica serovar Typhimurium-isozymes of different heat stability643748

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
SH-reagents have no effect on enzyme stabilityBos taurus-643730
reducing agent required for stabilityEntamoeba histolytica-642813
glycerol, 25% v/v stabilizes during purificationEscherichia coli-643738
enzyme requires 2-mercaptoethanol for stabilityHomo sapiens-643736
bovine serum albumin stabilizesHordeum vulgare subsp. vulgare-643739
enzyme becomes labile after affinity chromatography purification, probably due to diaggregationNeurospora crassa-643725
enzyme requires 2-mercaptoethanol for stabilityOryctolagus cuniculus-643736
stable to acid precipitationOryctolagus cuniculus-643733
no reducing agent required for stabilitySolanum tuberosum-643740
stable to dilution to the order of mg/lSolanum tuberosum-643740

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-20°C, crude acetone powder, desiccated storage, 2 yearsBos taurus-643730
PCMB, DTT, iodoacetate or 2-mercaptoethanol does not affect storage stabilityBos taurus-643730
-20°C, crude extract, 25% v/v glycerol, 3-4 weeksEscherichia coli-643738
0°C, unstable upon storageEscherichia coli-643748
-70°C, 50% glycerol, 2 months, no significant loss of activityPseudomonas aeruginosa-663272
4°C, Tris-HCl buffer, pH 7.5, several monthsSolanum tuberosum-643740
-20°C, 20% glycerol, stable at least 6 monthsStreptococcus pneumoniae-661546

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-Acanthamoeba castellanii-643745
immobilized metal ion adsorption chromatography; immobilized metal ion adsorption chromatographyArabidopsis thaliana-702416
metal chelation chromatographyArabidopsis thaliana-706224
-Bifidobacterium bifidum-642818
over 900foldBifidobacterium bifidum-642817
533fold, multiple formsBos taurus-643730
from liverBos taurus-643748
partialBos taurus-643731
from liverCapra hircus-643748
recombinant protein; recombinant proteinCorynebacterium glutamicumQ6M6R3677027
-Cucumis meloQ19TV8676614
partialCucumis sativus-642819
-Dictyostelium discoideum-643748
200foldEntamoeba histolytica-642813
recombinant from plasmid, 22.7foldEscherichia coli-643738
isozyme from Golgi apparatus, 95foldFelis catus-643734
-Helicobacter pylori-660635
from liverHomo sapiens-643748
recombinant wild-type enzyme, partially from Escherichia coli JM109Homo sapiens-643751
-Hordeum vulgare-663047
immobilized metal ion affinity chromatographyHordeum vulgare-702453
native and recombinant wild-type, the latter from Escherichia coli to homogeneityHordeum vulgareQ43772643757
partial, 367foldHordeum vulgare subsp. vulgare-643739
34foldLilium longiflorum-643729
500foldMus musculus-643726
immobilized metal affinity chromatographyMycobacterium tuberculosis H37RvO05576706591
to homogeneityNeurospora crassa-643725
2500fold to homogeneityOryctolagus cuniculus-643733
from liverOryctolagus cuniculus-643748
from liver; preparative sucrose density gradient centrifugationOryctolagus cuniculus-643736
-Oryza sativa-643724
from liverOvis aries-643748
-Pseudomonas aeruginosa-663272
partialRattus norvegicus-643748
salivary glands: 1 enzyme form, liver: 2 enzyme formsRattus norvegicus-643728
-Saccharomyces cerevisiae, Salmonella enterica subsp. enterica serovar Typhimurium-643748
177fold to near homogeneitySolanum tuberosum-643742
243fold to near homogeneitySolanum tuberosum-643740
ammonium sulfate precipitation, hydrophobic interaction chromatography, anion-exchange chromatography (DEAE)Solanum tuberosum-705362
partialSolanum tuberosum-643116
-Sorghum bicolor-643743
-Sphingomonas elodea-660765
-Streptococcus pneumoniae-661546
-Typha latifolia-643727
ammonium sulfate fractionation, ion-exchange chromatography (DEAE)Xanthomonas axonopodis pv. citriQ8PK83702480
ammonium sulfate fractionation, ion-exchange chromatography (DEAE)Xanthomonas campestris pv. campestrisQ4UVD0702480
-Zea mays-643748

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
expression in Agrobacterium tumefaciens; expression in Agrobacterium tumefaciensArabidopsis thalianaP57751, Q9M9P3700488
His-tagged version expressed in Escherichia coli BL21 (DE3); His-tagged version expressed in Escherichia coli BL21 (DE3)Arabidopsis thaliana-702416
His-tagged version expressed in Escherichia coli C41(DE3)Arabidopsis thaliana-706224
; Corynebacterium glutamicumQ6M6R3677027
-Cucumis meloQ19TV8676614
-Escherichia coliP0AEP3677032
galU structural gene, DNA sequence determination and analysis, expression from multicopy plasmidEscherichia coli-643738
expression in chondrocytes with or without co-transfection of HA synthase geneGallus gallus-643756
expression in Escherichia coliHelicobacter pylori-660635
expression of wild-type and mutants in Escherichia coli JM109Homo sapiens-643751
expression in Escherichia coliHordeum vulgare-663047
from cDNA libraries, 11 clones, DNA and amino acid sequence determination and analysisHordeum vulgare-643753
gene Ugp, expression in Escherichia coliHordeum vulgareQ43772643757
His-tag version expressed in Escherichia coli BL21 (DE3)Hordeum vulgare-702453
-Leishmania majorQ4QDU3674571
His-tagged version expressed in Escherichia coli BL21(DE3)Mycobacterium tuberculosis H37RvO05576706591
expression in Escherichia coliPseudomonas aeruginosa-663272
DNA sequence determination and analysis, gene disruption and depletion of activity is performed, overexpression from multicopy plasmid in haploid strains, on glucose 40fold increased enzyme activity leads to 2fold increase in the concentration of glycogen and UDP-D-glucose, on galactose a 40fold increased activity leads to several effects, e.g. 3fold reduction of growth rate, 3-5fold increase in UDP-D-glucose, UDP-D-galactose and alpha-D-galactose 1-phosphate concentrations, a higher sensitivity to calcofluor white and an increase in the degree of protein glycosylationSaccharomyces cerevisiae-643749
expressed in Escherichia coli BL21 starSolanum tuberosum-705362
from cDNA library, DNA sequence determination and analysisSolanum tuberosum-643744
-Sphingomonas elodea-671154
expression in Escherichia coliSphingomonas elodea-660765, 674334
expressed in Lactococcus lactis NZ9000Streptococcus equi subsp. zooepidemicusQ8GQP9701878
expression in Escherichia coliStreptococcus pneumoniae-661546
expressed in Escherichia coli BL21(DE3)Xanthomonas axonopodis pv. citriQ8PK83702480
expressed in Escherichia coli BL21(DE3)Xanthomonas campestris pv. campestrisQ4UVD0702480

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
phosphate starvationArabidopsis thaliana-706224

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
C123SHomo sapiens-site-directed mutagenesis, active enzyme, 7fold increase in Km for magnesium diphosphate, 2fold increased Ki for MgUTP2-, no longer sensitive to SH-reagents, e.g. iodoacetamide643751
H266RHomo sapiens-site-directed mutagenesis, mutant enzyme is active and similar to the wild-type, 4fold decrease in Km and Ki for MgUTP643751
R389HHomo sapiens-site-directed mutagenesis, mutant enzyme is active and similar to the wild-type643751
R391KHomo sapiens-site-directed mutagenesis, no activity, no correct folding643751
R422QHomo sapiens-site-directed mutagenesis, mutant enzyme is active and similar to the wild-type643751
R445HHomo sapiens-site-directed mutagenesis, mutant enzyme is active and similar to the wild-type643751
W218SHomo sapiens-site-directed mutagenesis, mutant enzyme is active and similar to the wild-type, increase in Km643751
W333SHomo sapiens-site-directed mutagenesis, no activity, no correct folding643751
C99SHordeum vulgareQ43772site-directed mutagenesis, half Vmax of wild-type, 12fold higher Km for diphosphate, altered diphosphate binding643757
Ccut-101Hordeum vulgare-101 amino acid residues deleted (exons 16-19, and 1 amino acid residue of exon 15)702453
Ccut-32Hordeum vulgare-32 amino acid residues deleted (exon 18 and exon 19)702453
Ccut-67Hordeum vulgare-67 amino acid residues deleted (exons 17-19)702453
Ccut-8Hordeum vulgare-8 amino acid residues deleted (last exon (exon 19))702453
Delta 1-4Hordeum vulgare-deletion of 4 amino acid residues of the C-terminal domain, possibly involved in oligomerization702453
Delta 1-8Hordeum vulgare-deletion of 8 amino acid residues of the C-terminal domain, possibly involved in oligomerization702453
Delta-NBHordeum vulgare-deletion mutant: amino acid residue 96-100 deleted (essential for catalysis)702453
K183AHordeum vulgare-possibly involved in subunit interaction702453
K260AHordeum vulgare-possibly involved in diphosphate binding702453
K332AHordeum vulgare-possibly involved in subunit interaction702453
Ncut-21Hordeum vulgare-21 amino acid residues deleted (exon 1)702453
Ncut-37Hordeum vulgare-37 amino acid residues deleted (exon 1 and exon 2)702453
Y192AHordeum vulgare-possibly involved in UDP-glucose binding702453
H191LLeishmania major-no residual activity674795
H191NLeishmania major-0.3% of wild-type activity674795
K380DLeishmania major-no residual activity674795
K95ALeishmania major-0.5% of wild-type activity674795
additional informationAeromonas hydrophila-enzyme gene disruption mutant reduces the survival of Aeromonas hydrophila in serum to less than 1%, decreases the ability of strains to adhere and reduces by 1.5 or 2 log units the virulence of Aeromonas serotype O34 strains in a septicaemia model. The mutant shows two types of lipopolysaccharide structures. The first one corresponds to a rough strain having the complete core, but lacking the terminal galactose residue from the LPS-core and 4-amino-4-deoxyarabinose residues from phosphate groups in lipid A. The second one corresponds to a deeply truncated structure with the LPS-core restricted to one 3-deoxy-D-manno-oct-2-ulosonic acid and three L-glycero-D-manno-heptose residues675852
additional informationArabidopsis thaliana-construction of transgenic plants, with increased or deleted expression of enzyme, study of sucrose effects on the enzyme and gene Ugp, respectively643755
K405AHordeum vulgare-possibly involved in subunit interaction702453
additional informationHordeum vulgareQ43772amino acid exchanges in hydrophobic domain643757
L281DLeishmania major-16.3% of wild-type activity674795
additional informationNicotiana tabacum-overexpression of sucrose synthase and of UDP-glucose pyrophosphorylase. Single transgenic plants show significant increases in the height growth. Double-transgene plants demonstrate an additive effect and are even taller than single-transgene parents. Double-transgene plants may show increases in soluble sugar content. Both enzymes may be markers for sink strength676407
D462HOryza sativaA3QQQ3natural mutation of isoform Ugp1, D462 in rice variety B5 is replaced by H462 in rice variety TN1676348
additional informationOryza sativaA3QQQ3isoform Ugp1 silencing by RNAi results in male sterility of plants. Pollen mother cells of silenced plants appear normal before meiosis, but during meiosis, normal callose deposition is disrupted and the degeneration of the tapetum and middle layer is inhibited676438

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
medicineHevea brasiliensis-UDP glucose diphosphorylase is an allergen in natural rubber latex and able to cause latex-fruit allergy syndroms. It may act as a potential pan-allergen in vegetable foods692142
synthesisHordeum vulgare subsp. vulgare-easy available enzyme can be used for synthesis of nucleotide sugars in enzymic glycoconjugate synthesis643752
agricultureNicotiana tabacum-overexpression of sucrose synthase and of UDP-glucose pyrophosphorylase. Single transgenic plants show significant increases in the height growth. Double-transgene plants demonstrate an additive effect and are even taller than single-transgene parents. Double-transgene plants may show increases in soluble sugar content. Both enzymes may be markers for sink strength676407

DISEASETITLE OF PUBLICATIONLINK TO PUBMED
CarcinomaUDPglucose pyrophosphorylase from Ehrlich ascites carcinoma cell--purification and characterization. PubMed
Diabetes MellitusHexose metabolism in pancreatic islets UDP-glucose pyrophosphorylase activity. PubMed
Diabetes Mellitus, Experimental[UDPG pyrophosphorylase and phosphoglucomutase activity in the liver of rats with alloxan diabetes] PubMed
Food HypersensitivityIdentification of latex UDP glucose pyrophosphorylase (Hev b UDPGP) as a novel cause of latex fruit allergy syndrome. PubMed
GalactosemiasUDP-galactose pyrophosphorylase in mice with galactose-1-phosphate uridyltransferase deficiency. PubMed
HypersensitivityIdentification of latex UDP glucose pyrophosphorylase (Hev b UDPGP) as a novel cause of latex fruit allergy syndrome. PubMed
NeoplasmsRole of nuclear glycogen synthase and cytoplasmic UDP glucose pyrophosphorylase in the biosynthesis of nuclear glycogen in HD33 Ehrlich-Lettré ascites tumor cells. PubMed
TuberculosisExpression, purification, and characterization of a functionally active Mycobacterium tuberculosis UDP-glucose pyrophosphorylase. PubMed

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISMLINK TO PUBMEDSOURCE
637084Kalckar, H.M.The role of phosphoglycosyl compounds in the biosynthesis of nucleosides and nucleotidesBiochim. Biophys. Acta12250-2641953Saccharomyces cerevisiae, Saccharomyces fragilis-
642813Lobelle-Rich, P.A.; Reeves, R.E.Separation and characterization of two UTP-utilizing hexose phosphate uridylyltransferases from Entamoeba histolyticaMol. Biochem. Parasitol.7173-1821983Entamoeba histolytica PubMed
642814Chacko, C.M.; McCrone, L.; Nadler, H.L.Uridine diphosphoglucose pyrophosphorylase and uridine diphosphogalactose pyrophosphorylase in human skin fibroblasts derived from normal and galactosemic individualsBiochim. Biophys. Acta268113-1201972Homo sapiens PubMed
642817Lee, L.J.; Kimura, A.; Tochikura, T.Presence of a single enzyme catalyzing the pyrophosphorolysis of UDP-glucose and UDP-galactose in Bifidobacterium bifidumBiochim. Biophys. Acta527301-3041978Bifidobacterium bifidum PubMed
642818Lee, L.J.; Kimura, A.; Tochikura, T.Purification and properties of UDP-glucose (UDP-galactose) pyrophosphorylase from Bifidobacterium bifidumJ. Biochem.86923-9281979Bifidobacterium bifidum PubMed
642819Smart, E.L.; Pharr, D.M.Separation and characteristics of galactose-1-phosphate and glucose-1-phosphate uridyltranferase from fruit peduncles of cucumberPlanta153370-3751981Cucumis sativus-
643116Sowokinos, J.R.Pyrophphosphorylases in Solanum tuberosum, II. Catalytic properties an reguation of ADP-glucose and UDP-glucose pyrophosphorylase activities in potatoesPlant Physiol.68924-9291981Solanum tuberosum PubMed
643724Kimura, S.; Mitsui, T.; Matsuoka, T.; Igaue, I.Purification, characterization and localization of rice UDP-glucose pyrophosphorylasePlant Physiol. Biochem.30683-6931992Oryza sativa-
643725Tovar, J.; Ruíz-Herrera, J.Purification and properties of glucose-1-phosphate uridylyltransferase from Neurospora crassaExp. Mycol.1136-481987Neurospora crassa-
643726Dutta, S.K.UDPglucose pyrophosphorylase from Ehrlich ascites carcinoma cell--purification and characterizationIndian J. Biochem. Biophys.22203-2071985Mus musculus PubMed
643727Hondo, T.; Hara, A.; Funaguma, T.The purification and some properties of the UDP-glucose pyrophosphorylase from pollen of Typha latifolia L.Plant Cell Physiol.2461-691983Typha latifolia-
643728Sadurska, B.; Szymczyk, T.Properties of UDPG pyrophosphorylase from rat salivary glands and liverArch. Oral Biol.23639-6461978Rattus norvegicus PubMed
643729Hopper, J.E.; Dickinson, D.B.Partial purification and sugar nucleotide inhibition of UDP-glucose pyrophosphorylase from Lilium longiflorum pollenArch. Biochem. Biophys.148523-5351972Lilium longiflorum PubMed
643730Aksamit, R.R.; Ebner, K.E.Purification, properties and kinetic analysis of UDP-glucose pyrophosphorylase from bovine mammary tissueBiochim. Biophys. Acta268102-1121972Bos taurus PubMed
643731Hopper, J.E.; Dickinson, D.B.Sugar nucleotide inhibition of UDP-glucose pyrophosphorylase from calf liverBiochim. Biophys. Acta309307-3171973Bos taurus PubMed
643732Turnquist, R.L.; Turnquist, M.M.; Bachmann, R.C.; Hansen, R.G.Uridine diphosphate glucose pyrophosphorylase: differential heat inactivation and further characterization of human liver enzymeBiochim. Biophys. Acta36459-671974Homo sapiens PubMed
643733Bergamini, C.; Signorini, M.; Ferrari, C.; Dallocchio, F.Purification of muscle uridine diphosphoglucose pyrophosphorylase by hydrophobic chromatographyAnal. Biochem.1335-381984Oryctolagus cuniculus-
643734Azzar, G.; Persat, F.; Degiuli, A.; Got, R.Purification of a UTP:D-glucose-1-phosphate uridylyltransferase from Golgi apparatus of cat liver by affinity chromatography on UTP-agaroseAnal. Biochem.142518-5201984Felis catus PubMed
643735Persat, F.; Azzar, G.; Martel, M.B.; Got, R.Properties of uridine diphosphate glucose pyrophosphorylase from Golgi apparatus of liverBiochim. Biophys. Acta749329-3321983Felis catus PubMed
643736Turnquist, R.L.; Gillett, T.A.; Hansen, R.G.Uridine diphosphate glucose pyrophosphorylase. Crystallization and properties of the enzyme from rabbit liver and species comparisonsJ. Biol. Chem.2497695-77001974Bos taurus, Homo sapiens, Oryctolagus cuniculus PubMed
643738Weissborn, A.C.; Liu, Q.; Rumley, M.K.; Kennedy, E.P.UTP: alpha-D-glucose-1-phosphate uridylyltransferase of Escherichia coli: isolation and DNA sequence of the galU gene and purification of the enzymeJ. Bacteriol.1762611-26181994Escherichia coli PubMed
643739Elling, L.; Kula, M.R.Purification of UDP-glucose pyrophosphorylase from germinated barley (malt)J. Biotechnol.34157-1631994Hordeum vulgare subsp. vulgare PubMed
643740Nakano, K.; Omura, Y.; Tagaya, M.; Fukui, T.UDP-glucose pyrophosphorylase from potato tuber: purification and characterizationJ. Biochem.106528-5321989Solanum tuberosum PubMed
643742Sowokinos, J.R.; Spychalla, J.P.; Desborough, S.L.Pyrophosphorylases in Solanum tuberosum. IV. Purification, tissue localization, and physicochemical properties of UDP-glucose pyrophosphorylasePlant Physiol.1011073-10801993Solanum tuberosum PubMed
643743Gustafson, G.L.; Gander, J.E.Uridine diphosphate glucose pyrophosphorylase from Sorghum vulgare. Purification and kinetic propertiesJ. Biol. Chem.2471387-13971972Sorghum bicolor PubMed
643744Katsube, T.; Kazuta, Y.; Mori, H.; Nakano, K.; Tanizawa, K.; Fukui, T.UDP-glucose pyrophosphorylase from potato tuber: cDNA cloning and sequencingJ. Biochem.108321-3261990Solanum tuberosum PubMed
643745Rudick, V.L.; Weisman, R.A.Uridine diphosphate glucose pyrophosphorylase of Acanthamoeba castellanii. Purification, kinetic, and developmental studiesJ. Biol. Chem.2497832-78401974Acanthamoeba castellanii PubMed
643746Nakae, T.; Nikaido, H.Multiple molecular forms of uridine diphosphate glucose pyrophosphorylase from Salmonella typhimurium. I. Catalytic properties of various formsJ. Biol. Chem.2464386-43961971Salmonella enterica subsp. enterica serovar Typhimurium PubMed
643747Dimond, R.L.; Farnsworth, P.A.; Loomis, W.F.Isolation and characterization of mutations affecting UDPG pyrophosphorylase activity in Dictyostelium discoideumDev. Biol.50169-1811976Dictyostelium discoideum PubMed
643748Turnquist, R.L.; Hansen, R.G.Uridine diphosphoryl glucose pyrophosphorylaseThe Enzymes, 3rd. Ed. (Boyer, P.D., ed.)851-711973Acetabularia sp., Beta vulgaris subsp. vulgaris var. altissima, Bombyx mori, Bos taurus, Canis lupus familiaris, Capra hircus, Columba sp., Dictyostelium discoideum, Escherichia coli, Gallus gallus, Homo sapiens, Oryctolagus cuniculus, Ovis aries, Pisum sativum, Rattus norvegicus, Saccharomyces cerevisiae, Salmonella enterica subsp. enterica serovar Typhimurium, Zea mays-
643749Daran, J.M.; Dallies, N.; Thines-Sempoux, D.; Paquet, V.; Francois, J.Genetic and biochemical characterization of the UGP1 gene encoding the UDP-glucose pyrophosphorylase from Saccharomyces cerevisiaeEur. J. Biochem.233520-5301995Saccharomyces cerevisiae PubMed
643750Lightcap, E.S.; Frey, P.A.m-Monothiopyrophosphate as a substrate for inorganic pyrophosphatase and UDP-glucose pyrophosphorylaseArch. Biochem. Biophys.335183-1901996Saccharomyces cerevisiae PubMed
643751Chang, H.Y.; Peng, H.L.; Chao, Y.C.; Duggleby, R.G.The importance of conserved residues in human liver UDPglucose pyrophosphorylaseEur. J. Biochem.236723-7281996Homo sapiens PubMed
643752Elling, L.Kinetic characterization of UDP-glucose pyrophosphorylase from germinated barley (malt)Phytochemistry42955-9601996Hordeum vulgare subsp. vulgare-
643753Eimert, K.; Villand, P.; Kilian, A.; Kleczkowski, L.A.Cloning and characterization of several cDNAs for UDP-glucose pyrophosphorylase from barley (Hordeum vulgare) tissuesGene170227-2321996Hordeum vulgare PubMed
643754Degeest, B.; De Vuyst, L.Correlation of activities of the enzymes a-phosphoglucomutase, UDP-galactose 4-epimerase, and UDP-glucose pyrophosphorylase with exopolysaccharide biosynthesis by Streptococcus thermophilus LY03Appl. Environ. Microbiol.663519-35272000Streptomyces thermophilus PubMed
643755Ciereszko, I.; Johansson, H.; Kleczkowski, L.A.Sucrose and light regulation of a cold-inducible UDP-glucose pyrophosphorylase gene via a hexokinase-independent and abscisic acid-insensitive pathway in ArabidopsisBiochem. J.35467-722001Arabidopsis thaliana PubMed
643756Magee, C.; Nurminskaya, M.; Linsenmayer, T.F.UDP-glucose pyrophosphorylase: up-regulation in hypertrophic cartilage and role in hyaluronan synthesisBiochem. J.360667-6742001Gallus gallus PubMed
643757Martz, F.; Wilczynska, M.; Kleczkowski, L.A.Oligomerization status, with the monomer as active species, defines catalytic efficiency of UDP-glucose pyrophosphorylaseBiochem. J.367295-3002002Hordeum vulgare PubMed
660635Kim, H.; Wu, C.A.; Kim, D.Y.; Han, Y.H.; Ha, S.C.; Kim, C.S.; Suh, S.W.; Kim, K.K.Crystallization and preliminary X-ray crystallographic study of UDP-glucose pyrophosphorylase (UGPase) from Helicobacter pyloriACTA CRYSTALLOGR.SECT.D601447-14492004Helicobacter pylori PubMed
660765Silva, E.; Marques, A.R.; Fialho, A.M.; Granja, A.T.; Sa-Correia, I.Proteins encoded by Sphingomonas elodea ATCC 31461 rmlA and ugpG genes, involved in gellan gum biosynthesis, exhibit both dTDP- and UDP-glucose pyrophosphorylase activitiesAppl. Environ. Microbiol.714703-47122005Sphingomonas elodea PubMed
661546Bonofiglio, L.; Garcia, E.; Mollerach, M.Biochemical characterization of the pneumococcal glucose 1-phosphate uridylyltransferase (GalU) essential for capsule biosynthesisCurr. Microbiol.51217-2212005Streptococcus pneumoniae PubMed
661732Guranowski, A.; de Diego, A.; Sillero, A.; Gunther Sillero, M.A.Uridine 5'-polyphosphates (p4U and p5U) and uridine(5')polyphospho(5')nucleosides (Up(n)Ns) can be synthesized by UTP:glucose-1-phosphate uridylyltransferase from Saccharomyces cerevisiaeFEBS Lett.56183-882004Saccharomyces cerevisiae PubMed
662442Reynolds, T.H.t.; Pak, Y.; Harris, T.E.; Manchester, J.; Barrett, E.J.; Lawrence, J.C., Jr.Effects of insulin and transgenic overexpression of UDP-glucose pyrophosphorylase on UDP-glucose and glycogen accumulation in skeletal muscle fibersJ. Biol. Chem.2805510-55152005Mus musculus PubMed
662716Ciereszko, I.; Johansson, H.; Kleczkowski, L.A.Interactive effects of phosphate deficiency, sucrose and light/dark conditions on gene expression of UDP-glucose pyrophosphorylase in ArabidopsisJ. Plant Physiol.162343-3532005Arabidopsis thaliana PubMed
663047Kleczkowski, L.A.; Martz, F.; Wilczynska, M.Factors affecting oligomerization status of UDP-glucose pyrophosphorylasePhytochemistry662815-28212005Hordeum vulgare PubMed
663272Chang, H.Y.; Huang, H.C.; Lee, J.H.; Peng, H.L.Characterization of a putative Pseudomonas UDPglucose pyrophosphorylaseProc. Nat. Sci. Counc. Repub. China Part B Life Sci.2374-841999Pseudomonas aeruginosa PubMed
663369Hedegaard, J.; Horn, P.; Lametsch, R.; Sondergaard Moller, H.; Roepstorff, P.; Bendixen, C.; Bendixen, E.UDP-glucose pyrophosphorylase is upregulated in carriers of the porcine RN- mutation in the AMP-activated protein kinaseProteomics42448-24542004Sus scrofa PubMed
671154Aragao, D.; Marques, A.R.; Frazao, C.; Enguita, F.J.; Carrondo, M.A.; Fialho, A.M.; Sa-Correia, I.; Mitchell, E.P.Cloning, expression, purification, crystallization and preliminary structure determination of glucose-1-phosphate uridylyltransferase (UgpG) from Sphingomonas elodea ATCC 31461 bound to glucose-1-phosphateActa Crystallogr. Sect. FF62930-9342006Sphingomonas elodea PubMed
673839Meng, M.; Geisler, M.; Johansson, H.; Mellerowicz, E.J.; Karpinski, S.; Kleczkowski, L.A.Differential tissue/organ-dependent expression of two sucrose- and cold-responsive genes for UDP-glucose pyrophosphorylase in PopulusGene389186-1952007Populus tremula x Populus tremuloides PubMed
674334Aragao, D.; Fialho, A.M.; Marques, A.R.; Mitchell, E.P.; Sa-Correia, I.; Frazao, C.The complex of Sphingomonas elodea ATCC 31461 glucose-1-phosphate uridylyltransferase with glucose-1-phosphate reveals a novel quaternary structure, unique among nucleoside diphosphate-sugar pyrophosphorylase membersJ. Bacteriol.1894520-45282007Sphingomonas elodea PubMed
674571Lamerz, A.C.; Haselhorst, T.; Bergfeld, A.K.; von Itzstein, M.; Gerardy-Schahn, R.Molecular cloning of the Leishmania major UDP-glucose pyrophosphorylase, functional characterization, and ligand binding analyses using NMR spectroscopyJ. Biol. Chem.28116314-163222006Leishmania major PubMed
674795Steiner, T.; Lamerz, A.C.; Hess, P.; Breithaupt, C.; Krapp, S.; Bourenkov, G.; Huber, R.; Gerardy-Schahn, R.; Jacob, U.Open and closed structures of the UDP-glucose pyrophosphorylase from Leishmania majorJ. Biol. Chem.28213003-130102007Leishmania major PubMed
675332Seo, M.; Im, E.; Singh, D.; Rajkarnikar, A.; Kwon, H.; Hyun, C.; Suh, J.; Pyun, Y.; Kim, S.Characterization of D-glucose alpha -1-phosphate uridylyltransferase (VldB) and glucokinase (VldC) involved in validamycin biosynthesis of Streptomyces hygroscopicus var. limoneus KCCM 11405J. Microbiol. Biotechnol.161311-13152006Streptomyces hygroscopicus-
675416Roeben, A.; Plitzko, J.M.; Koerner, R.; Boettcher, U.M.; Siegers, K.; Hayer-Hartl, M.; Bracher, A.Structural basis for subunit assembly in UDP-glucose pyrophosphorylase from Saccharomyces cerevisiaeJ. Mol. Biol.364551-5602006Saccharomyces cerevisiae PubMed
675432McCoy, J.G.; Bitto, E.; Bingman, C.A.; Wesenberg, G.E.; Bannen, R.M.; Kondrashov, D.A.; Phillips, G.N.Structure and dynamics of UDP-glucose pyrophosphorylase from Arabidopsis thaliana with bound UDP-glucose and UTPJ. Mol. Biol.366830-8412007Arabidopsis thaliana PubMed
675852Vilches, S.; Canals, R.; Wilhelms, M.; Salo, M.T.; Knirel, Y.A.; Vinogradov, E.; Merino, S.; Tomas, J.M.Mesophilic Aeromonas UDP-glucose pyrophosphorylase (GalU) mutants show two types of lipopolysaccharide structures and reduced virulenceMicrobiology1532393-24042007Aeromonas hydrophila PubMed
676348Chen, R.; Zhao, X.; Shao, Z.; Zhu, L.; He, G.Multiple isoforms of UDP-glucose pyrophosphorylase in ricePhysiol. Plant.129725-7362007Oryza sativa-
676407Coleman, H.D.; Ellis, D.D.; Gilbert, M.; Mansfield, S.D.Up-regulation of sucrose synthase and UDP-glucose pyrophosphorylase impacts plant growth and metabolismPlant Biotechnol. J.487-1012006Nicotiana tabacum PubMed
676438Chen, R.; Zhao, X.; Shao, Z.; Wei, Z.; Wang, Y.; Zhu, L.; Zhao, J.; Sun, M.; He, R.; He, G.Rice UDP-glucose pyrophosphorylase1 is essential for pollen callose deposition and its cosuppression results in a new type of thermosensitive genic male sterilityPlant Cell19847-8612007Oryza sativa PubMed
676614Dai, N.; Petreikov, M.; Portnoy, V.; Katzir, N.; Pharr, D.M.; Schaffer, A.A.Cloning and expression analysis of a UDP-galactose/glucose pyrophosphorylase from melon fruit provides evidence for the major metabolic pathway of galactose metabolism in raffinose oligosaccharide metabolizing plantsPlant Physiol.142294-3042006Cucumis melo PubMed
677027Thoden, J.B.; Holden, H.M.Active site geometry of glucose-1-phosphate uridylyltransferaseProtein Sci.161379-13882007Corynebacterium glutamicum PubMed
677032Thoden, J.B.; Holden, H.M.The molecular architecture of glucose-1-phosphate uridylyltransferaseProtein Sci.16432-4402007Escherichia coli PubMed
692142Conti, A.; Giuffrida, M.G.; Hoffmann-Sommergruber, K.; Wagner, S.; Amato, S.; Mistrello, G.; Asero, R.Identification of latex UDP glucose pyrophosphorylase (Hev b UDPGP) as a novel cause of latex fruit allergy syndromeEur. Ann. Allergy Clin. Immunol.39116-1182007Hevea brasiliensis PubMed
700488Siddique, S.; Endres, S.; Atkins, J.M.; Szakasits, D.; Wieczorek, K.; Hofmann, J.; Blaukopf, C.; Urwin, P.E.; Tenhaken, R.; Grundler, F.M.; Kreil, D.P.; Bohlmann, H.Myo-inositol oxygenase genes are involved in the development of syncytia induced by Heterodera schachtii in Arabidopsis rootsNew Phytol.184457-4722009Arabidopsis thaliana PubMed
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LINKS TO OTHER DATABASES (specific for EC-Number 2.7.7.9)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)