Information on EC 2.7.7.75 - molybdopterin adenylyltransferase

Information on EC 2.7.7.75 - molybdopterin adenylyltransferase:

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EC NUMBER	COMMENTARY
2.7.7.75	-

RECOMMENDED NAME	GeneOntology No.
molybdopterin adenylyltransferase	-

REACTION	REACTION DIAGRAM	COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
ATP + molybdopterin = diphosphate + adenylyl-molybdopterin		-	Escherichia coli	P0AF03	708986
ATP + molybdopterin = diphosphate + adenylyl-molybdopterin		-	Arabidopsis thaliana	-	710443
ATP + molybdopterin = diphosphate + adenylyl-molybdopterin		-	-	-	-

REACTION TYPE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
nucleotidyl group transfer	Escherichia coli	P0AF03	-	708986
nucleotidyl group transfer	Arabidopsis thaliana	Q39054	-	708983, 710443

PATHWAY	KEGG Link	MetaCyc Link
molybdenum cofactor biosynthesis	-	PWY-6823

SYSTEMATIC NAME	IUBMB Comments
ATP:molybdopterin adenylyltransferase	Catalyses the activation of molybdopterin for molybdenum insertion. In eukaryotes, this reaction is catalysed by the C-terminal domain of a fusion protein that also includes molybdopterin molybdotransferase (EC 2.10.1.1). The reaction requires a divalent cation such as Mg2+ or Mn2+.

SYNONYMS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
Cnx1	Arabidopsis thaliana	Q39054	C-terminal G-domain, gene name	708983, 710443
MogA	Escherichia coli	P0AF03	gene name	708986

CAS REGISTRY NUMBER	COMMENTARY
No entries in this field

ORGANISM	COMMENTARY	LITERATURE	SEQUENCE CODE	SEQUENCE DB	SOURCE
Arabidopsis thaliana	domain G; commentary	708983, 710443	Q39054	SwissProt
Escherichia coli	K-12	708986	P0AF03	SwissProt

GENERAL INFORMATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
metabolism	Escherichia coli	P0AF03	molybdenum cofactor biosynthesis	708986
metabolism	Arabidopsis thaliana	Q39054	molybdenum cofactor biosynthesis	708983, 710443

SUBSTRATE	PRODUCT	REACTION DIAGRAM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY/ Substrate	LITERATURE/ Substrate	COMMENTARY/ Product	LITERATURE/ Product	Reversibility r=reversible ir=irreversible ?=not specified
ATP + molybdopterin	diphosphate + adenylyl-molybdopterin		Arabidopsis thaliana	Q39054	-	708983	-	-	?
ATP + molybdopterin	diphosphate + adenylyl-molybdopterin		Arabidopsis thaliana	-	-	710443	-	-	?
ATP + molybdopterin	diphosphate + adenylyl-molybdopterin		Escherichia coli	P0AF03	-	708986	-	-	?

NATURAL SUBSTRATES	NATURAL PRODUCTS	REACTION DIAGRAM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY SUBSTRATE	LITERATURE (Substrate)	COMMENTARY PRODUCT	LITERATURE (Product)
ATP + molybdopterin	diphosphate + adenylyl-molybdopterin		Arabidopsis thaliana	Q39054	-	708983	-	-
ATP + molybdopterin	diphosphate + adenylyl-molybdopterin		Arabidopsis thaliana	-	-	710443	-	-
ATP + molybdopterin	diphosphate + adenylyl-molybdopterin		Escherichia coli	P0AF03	-	708986	-	-

COFACTOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

METALS and IONS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
Mg2+	Arabidopsis thaliana	-	required	708983
Mg2+	Escherichia coli	P0AF03	required	708986
Mn2+	Arabidopsis thaliana	-	required	708983

INHIBITORS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
diphosphate	Arabidopsis thaliana	-	efficient inhibition at 1-10 mM diphosphate	708983	2D-image

ACTIVATING COMPOUND	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
diphosphate	Arabidopsis thaliana	-	activating by 5 units in the standard reaction set-up	708983	2D-image

KM VALUE [mM]	KM VALUE [mM] Maximum	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
0.062	-	ATP	Arabidopsis thaliana	-	10 mM MgCl2, pH 7.2, temperature not specified in the publication	708983	2D-image

TURNOVER NUMBER [1/s]	TURNOVER NUMBER MAXIMUM[1/s]	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
0.0012	-	ATP	Arabidopsis thaliana	-	10 mM MgCl2, pH 7.2, temperature not specified in the publication	708983	2D-image

kcat/KM VALUE [1/mMs^-1]	kcat/KM VALUE [1/mMs^-1] Maximum	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

Ki VALUE [mM]	Ki VALUE [mM] Maximum	INHIBITOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

IC50 VALUE [mM]	IC50 VALUE [mM] Maximum	INHIBITOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg]	SPECIFIC ACTIVITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

pH OPTIMUM	pH MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

pH RANGE	pH RANGE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

TEMPERATURE OPTIMUM	TEMPERATURE OPTIMUM MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

TEMPERATURE RANGE	TEMPERATURE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

pI VALUE	pI VALUE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

SOURCE TISSUE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	SOURCE
No entries in this field

LOCALIZATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	GeneOntology No.	LITERATURE	SOURCE
No entries in this field

PDB	SCOP	CATH	ORGANISM
No entries in this field

MOLECULAR WEIGHT	MOLECULAR WEIGHT MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

SUBUNITS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

POSTTRANSLATIONAL MODIFICATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

Crystallization/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

pH STABILITY	pH STABILITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

TEMPERATURE STABILITY	TEMPERATURE STABILITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

GENERAL STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

ORGANIC SOLVENT	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

OXIDATION STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

STORAGE STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

Purification/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
Ni-nitrilotriacetic acid matrix	Arabidopsis thaliana	Q39054	708983, 710443

Cloned/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
Cnx1 G-domain in pQE60	Arabidopsis thaliana	Q39054	708983, 710443

EXPRESSION	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

ENGINEERING	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
D486A	Arabidopsis thaliana	-	inactive	708983
D515H	Arabidopsis thaliana	-	inactive	708983
D515H	Arabidopsis thaliana	-	in Cnx1 G-domain, accumulation of molybdopterin	710443
D515N	Arabidopsis thaliana	-	in Cnx1 G-domain, accumulation of molybdopterin	710443
N597L	Arabidopsis thaliana	-	in Cnx1 G-domain, strong decrease in molybdopterin binding	710443
R547E	Arabidopsis thaliana	-	decreased activity	708983
S476A	Arabidopsis thaliana	-	inactive	708983
S573A	Arabidopsis thaliana	-	inactive in absence of pyrophosphatase, decreased actitivity in presence of pyrophosphatase	708983
S583A	Arabidopsis thaliana	-	increased activity	708983
T542A	Arabidopsis thaliana	-	decreased activity	708983
T542D	Arabidopsis thaliana	-	inactive	708983
T542S	Arabidopsis thaliana	-	decreased activity	708983
V557G	Arabidopsis thaliana	-	in Cnx1 G-domain, strong decrease in molybdopterin binding	710443

Renatured/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

APPLICATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

REF.	AUTHORS	TITLE	JOURNAL	VOL.	PAGES	YEAR	ORGANISM (UNIPROT ACCESSION NO.)	LINK TO PUBMED	SOURCE
708983	Llamas, A.; Mendel, R.R.; Schwarz, G.	Synthesis of adenylated molybdopterin: an essential step for molybdenum insertion	J. Biol. Chem.	279	55241-55246	2004	Arabidopsis thaliana, Arabidopsis thaliana (Q39054)	PubMed
708986	Nichols, J.D.; Rajagopalan, K.V.	In vitro molybdenum ligation to molybdopterin using purified components	J. Biol. Chem.	280	7817-7822	2005	Escherichia coli (P0AF03), Escherichia coli (P12281)	PubMed
710443	Kuper, J.; Palmer, T.; Mendel, R.R.; Schwarz, G.	Mutations in the molybdenum cofactor biosynthetic protein Cnx1G from Arabidopsis thaliana define functions for molybdopterin binding, molybdenum insertion, and molybdenum cofactor stabilization	Proc. Natl. Acad. Sci. USA	97	6475-6480	2000	Arabidopsis thaliana (Q39054)	PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 2.7.7.75)

NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM

IUBMB Enzyme Nomenclature

PROSITE Database of protein families and domains

Protein Mutant Database

InterPro (database of protein families, domains and functional sites)