Information on EC 2.7.2.1 - acetate kinase:
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EC NUMBERCOMMENTARY
2.7.2.1-

RECOMMENDED NAMEGeneOntology No.
acetate kinaseGO:0008776

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
ATP + acetate = ADP + acetyl phosphate
show the reaction diagram		propanoate also acts as acceptor, but more slowly---
ATP + acetate = ADP + acetyl phosphate
show the reaction diagram		residue F179 is essential for catalysisMethanosarcina thermophila-659065
ATP + acetate = ADP + acetyl phosphate
show the reaction diagram		roles for the arginine residues Arg241 and Arg91 in transition state stabilization for catalysis but not in nucleotide binding determined, experimental evidence for domain motionMethanosarcina thermophila-690888
ATP + propanoate = ADP + propanoyl phosphate
show the reaction diagram		----
ATP + propanoate = ADP + propanoyl phosphate
show the reaction diagram		-Methanosarcina thermophila-659065

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
phospho group transfer----

PATHWAYKEGG LinkMetaCyc Link
acetate formation from acetyl-CoA I-PWY0-1312
acetylene degradation-P161-PWY
Bifidobacterium shunt-P124-PWY
gallate degradation III (anaerobic)-P3-PWY
lysine fermentation to acetate and butyrate-P163-PWY
methanogenesis from acetate-METH-ACETATE-PWY
mixed acid fermentation-FERMENTATION-PWY
purine nucleotides degradation III (anaerobic)-P164-PWY
purine nucleotides degradation IV (anaerobic)-PWY-5497
pyruvate fermentation to acetate II-PWY-5482
pyruvate fermentation to acetate IV-PWY-5485

SYSTEMATIC NAMEIUBMB Comments
ATP:acetate phosphotransferaseRequires Mg2+ for activity. While purified enzyme from Escherichia coli is specific for acetate [4], others have found that the enzyme can also use propanoate as a substrate, but more slowly [7]. Acetate can be converted into the key metabolic intermediate acetyl-CoA by coupling acetate kinase with EC 2.3.1.8, phosphate acetyltransferase. Both this enzyme and EC 2.7.2.15, propionate kinase, play important roles in the production of propanoate [9].

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
acetate kinase (phosphorylating)----
acetic kinase----
acetokinase----
ACKPropionibacterium acidipropioniciQ5BM21-672708
ACKEthanoligenens harbinenseQ2N1I6-673209
ACKRoseovarius sp.--673750
AK----

CAS REGISTRY NUMBERCOMMENTARY
9027-42-3-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Acholeplasma laidlawiioral strain642176--Manually annotated by BRENDA team
Acholeplasma laidlawii oraloral strain642176--Manually annotated by BRENDA team
Acinetobacter calcoaceticus-642185--Manually annotated by BRENDA team
Ajellomyces capsulatus-677237--Manually annotated by BRENDA team
Aspergillus fumigatus-677237--Manually annotated by BRENDA team
Bacillus subtilis-677237--Manually annotated by BRENDA team
Borrelia burgdorferi-677237--Manually annotated by BRENDA team
Chaetomium globosum-677237--Manually annotated by BRENDA team
Chlamydomonas reinhardtii-677237--Manually annotated by BRENDA team
Clostridium acetobutylicumDSM 1731 and DSM 792642189--Manually annotated by BRENDA team
Clostridium acetobutylicumP262642187--Manually annotated by BRENDA team
Clostridium thermocellumATCC 27405642190--Manually annotated by BRENDA team
Coccidioides immitis-677237--Manually annotated by BRENDA team
Coprinopsis cinerea-677237--Manually annotated by BRENDA team
Cryptococcus neoformans-677237--Manually annotated by BRENDA team
Cupriavidus necator-677237--Manually annotated by BRENDA team
Desulfovibrio vulgarisMiyazaki F.642197--Manually annotated by BRENDA team
Desulfovibrio vulgarissubsp. vulgaris, DSM 644, NCIB strain 8303642186--Manually annotated by BRENDA team
Desulfovibrio vulgaris Miyazaki F.Miyazaki F.642197--Manually annotated by BRENDA team
Emericella nidulans-677237--Manually annotated by BRENDA team
Entamoeba histolytica-677237--Manually annotated by BRENDA team
Enterococcus faecalis-677237--Manually annotated by BRENDA team
Escherichia coli-642166, 642167, 642168, 642169, 642177, 659056--Manually annotated by BRENDA team
Escherichia coliK235, 1100 thi-642182--Manually annotated by BRENDA team
Ethanoligenens harbinensestrain hit B49673209Q2N1I6TrEMBLManually annotated by BRENDA team
Geobacillus stearothermophilus-642198--Manually annotated by BRENDA team
Geobacillus stearothermophilusNCA 1503642172, 642180--Manually annotated by BRENDA team
Gibberella zeae-677237--Manually annotated by BRENDA team
Helicobacter pylori-677237--Manually annotated by BRENDA team
Hypocrea jecorina-677237--Manually annotated by BRENDA team
Lactobacillus acidophilus-677237--Manually annotated by BRENDA team
Lactobacillus delbrueckii-642166--Manually annotated by BRENDA team
Lactobacillus sanfranciscensisDSM20451, same nucleotide sequence accession number in EMBL/GenBank642195Q9F1X7SwissProtManually annotated by BRENDA team
Lactococcus lactis-677237--Manually annotated by BRENDA team
Listeria monocytogenes-677237--Manually annotated by BRENDA team
Magnaporthe grisea-677237--Manually annotated by BRENDA team
Mesoplasma florum-677237--Manually annotated by BRENDA team
Methanosarcina acetivorans-677237--Manually annotated by BRENDA team
Methanosarcina mazei-677237--Manually annotated by BRENDA team
Methanosarcina thermophila-642184, 642188, 642191, 642193, 642194, 642196, 659065, 659856, 677237--Manually annotated by BRENDA team
Methanosarcina thermophila-659446, 690888P38502SwissProtManually annotated by BRENDA team
Moorella thermoacetica-642170--Manually annotated by BRENDA team
Mycoplasma pneumoniae-677237--Manually annotated by BRENDA team
Mycoplasma pneumoniaewild-type strain M129 and mutant strains693619P75245SwissProtManually annotated by BRENDA team
Neurospora crassa-677237--Manually annotated by BRENDA team
Oceanobacillus iheyensis-677237--Manually annotated by BRENDA team
Phaeosphaeria nodorum-677237--Manually annotated by BRENDA team
Phanerochaete chrysosporium-677237--Manually annotated by BRENDA team
Phytophthora ramorum-677237--Manually annotated by BRENDA team
Phytophthora sojae-677237--Manually annotated by BRENDA team
Propionibacterium acidipropionicistrain ATCC 4875672708Q5BM21TrEMBLManually annotated by BRENDA team
Propionibacterium freudenreichiiIFO 12424642181--Manually annotated by BRENDA team
Rhodopseudomonas palustris-642183--Manually annotated by BRENDA team
Roseovarius sp.strain 217673750--Manually annotated by BRENDA team
Salmonella enterica-677237--Manually annotated by BRENDA team
Salmonella enterica subsp. enterica serovar TyphimuriumLT2, wild-type642182--Manually annotated by BRENDA team
Sclerotinia sclerotiorum-677237--Manually annotated by BRENDA team
Spirochaeta sp.-642179--Manually annotated by BRENDA team
Staphylococcus aureus-677237--Manually annotated by BRENDA team
Streptococcus pneumoniae-677237--Manually annotated by BRENDA team
Streptococcus pyogenes-642166--Manually annotated by BRENDA team
Thermotoga maritima-487537--Manually annotated by BRENDA team
Thermotoga maritima-691204Q9WYB1SwissProtManually annotated by BRENDA team
Uncinocarpus reesii-677237--Manually annotated by BRENDA team
Ustilago maydis-677237--Manually annotated by BRENDA team
Veillonella parvula-642171, 642174, 642175--Manually annotated by BRENDA team
Veillonella parvulaATCC 17748642173--Manually annotated by BRENDA team
Veillonella parvulastrain 221 (ATCC 17745)642178--Manually annotated by BRENDA team
Veillonella parvula 221strain 221 (ATCC 17745)642178--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
acetyl phosphate + hydroxylamineacetyl hydroxamate + phosphate
show the reaction diagram		Methanosarcina thermophila--659065--r
acetyl phosphate + hydroxylamineacetyl hydroxamate + phosphate
show the reaction diagram		Methanosarcina thermophilaP38502-659446--r
acetyl phosphate + hydroxylamineacetyl hydroxamate + phosphate
show the reaction diagram		Methanosarcina thermophila--659856--r
acetyl phosphate + hydroxylamineacetyl hydroxamate + phosphate
show the reaction diagram		Thermotoga maritimaQ9WYB1high-energy acetyl phosphate reacts with hydroxylamine forming acetyl hydroxamate and inorganic phosphate691204--?
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Salmonella enterica subsp. enterica serovar Typhimurium--642182-642182r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Staphylococcus aureus, Chlamydomonas reinhardtii, Bacillus subtilis--677237--?
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Escherichia coli--642166-642166r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Escherichia coli--642167-642167r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Escherichia coli--642177-642177r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Escherichia coli--642182-642182r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Geobacillus stearothermophilus--642172-642172r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Geobacillus stearothermophilus--642180-642180r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Geobacillus stearothermophilus--642198-642198r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Emericella nidulans, Neurospora crassa, Entamoeba histolytica, Lactococcus lactis--677237--?
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Rhodopseudomonas palustris--642183-642183r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Acinetobacter calcoaceticus--642185-642185r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Enterococcus faecalis, Streptococcus pneumoniae--677237--?
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Streptococcus pyogenes--642166-642166r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Moorella thermoacetica--642170-642170r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Veillonella parvula--642171-642171r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Veillonella parvula--642173-642173r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Veillonella parvula--642174-642174r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Veillonella parvula--642175-642175r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Veillonella parvula--642178-642178r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Coprinopsis cinerea, Helicobacter pylori--677237--?
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Lactobacillus delbrueckii--642166-642166r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Cupriavidus necator--677237--?
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Acholeplasma laidlawii--642176-642176r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Salmonella enterica--677237--?
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Propionibacterium freudenreichii--642181-642181r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Ustilago maydis, Sclerotinia sclerotiorum, Cryptococcus neoformans--677237--?
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Clostridium acetobutylicum--642189-642189r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Lactobacillus acidophilus--677237--?
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Desulfovibrio vulgaris--642186-642186r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Desulfovibrio vulgaris--642197-642197r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Magnaporthe grisea, Methanosarcina thermophila--677237--?
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Methanosarcina thermophila--642184-642184r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Methanosarcina thermophila--642191-642191r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Methanosarcina thermophila--642194-642194r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Methanosarcina thermophila--642196-642196r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Methanosarcina thermophila--659065--r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Methanosarcina thermophilaP38502-659446--r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Methanosarcina thermophila--659856--r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Hypocrea jecorina, Phanerochaete chrysosporium--677237--?
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Thermotoga maritima--487537-487537r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Aspergillus fumigatus, Mycoplasma pneumoniae, Gibberella zeae, Chaetomium globosum, Listeria monocytogenes--677237--?
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Clostridium thermocellum--642190-642190r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Coccidioides immitis, Methanosarcina mazei, Borrelia burgdorferi, Ajellomyces capsulatus, Phytophthora sojae, Mesoplasma florum--677237--?
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Lactobacillus sanfranciscensisQ9F1X7-642195-642195r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Spirochaeta sp.--642179-642179r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Methanosarcina acetivorans, Phaeosphaeria nodorum, Oceanobacillus iheyensis, Uncinocarpus reesii--677237--?
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Propionibacterium acidipropioniciQ5BM21-672708--?
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Ethanoligenens harbinenseQ2N1I6-673209--?
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Roseovarius sp.--673750--?
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Phytophthora ramorum--677237--?
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Methanosarcina thermophila--690888--r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Thermotoga maritimaQ9WYB1-691204--?
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Mycoplasma pneumoniaeP75245-693619--?
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Geobacillus stearothermophilus-function in the metabolism of pyruvate or synthesis of acetyl-CoA coupling with phosphoacetyltransacetylase642180-642180r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Escherichia coli-acetate kinase/phosphotransacetylase, major role of this two-enzyme sequence is to provide acetyl coenzyme A which may participate in fatty acid synthesis, citrate formation and subsequent oxidation642166-642166r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Lactobacillus sanfranciscensisQ9F1X7key enzyme and responsible for dephosphorylation of acetyl phosphate with the concomitant production of acetate and ATP642195-642195r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Methanosarcina thermophila-function in the initial activation of acetate for conversion to methane and CO2642184-642184r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Methanosarcina thermophila-catalytic mechanism, roles for the arginine residues Arg241 and Arg91 in transition state stabilization for catalysis but not in nucleotide binding determined, experimental evidence for domain motion690888--r
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Thermotoga maritimaQ9WYB1enzyme assay developed that can measure the released phosphate at a nanomolar level691204--?
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Mycoplasma pneumoniaeP75245transcriptional control of the ackA gene693619--?
ATP + butyrateADP + butyryl phosphate
show the reaction diagram		Rhodopseudomonas palustris--642183-642183r
ATP + butyrateADP + butyryl phosphate
show the reaction diagram		Methanosarcina thermophila--659065--r
ATP + butyrateADP + butyryl phosphate
show the reaction diagram		Methanosarcina thermophila-2% of reactivity with acetate659856--r
ATP + ethanolADP + ethyl phosphate
show the reaction diagram		Methanosarcina thermophila-1% of reactivity with acetate659856--r
ATP + formateADP + formyl phosphate
show the reaction diagram		Methanosarcina thermophila-2% of reactivity with acetate659856--r
ATP + glycerolADP + glycerol phosphate
show the reaction diagram		Methanosarcina thermophila-1% of reactivity with acetate659856--r
ATP + glycineADP + glycyl phosphate
show the reaction diagram		Methanosarcina thermophila-1% of reactivity with acetate659856--r
ATP + glycolic acidADP + ?
show the reaction diagram		Methanosarcina thermophila-4% of reactivity with acetate659856--r
ATP + isobutyrateADP + isobutyryl phosphate
show the reaction diagram		Rhodopseudomonas palustris--642183-642183r
ATP + propionateADP + propionyl phosphate
show the reaction diagram		Geobacillus stearothermophilus--642172-642172r
ATP + propionateADP + propionyl phosphate
show the reaction diagram		Rhodopseudomonas palustris--642183-642183r
ATP + propionateADP + propionyl phosphate
show the reaction diagram		Veillonella parvula--642171-642171r
ATP + propionateADP + propionyl phosphate
show the reaction diagram		Veillonella parvula--642173-642173r
ATP + propionateADP + propionyl phosphate
show the reaction diagram		Veillonella parvula--642178-642178r
ATP + propionateADP + propionyl phosphate
show the reaction diagram		Acholeplasma laidlawii--642176-642176r
ATP + propionateADP + propionyl phosphate
show the reaction diagram		Propionibacterium freudenreichii--642181-642181r
ATP + propionateADP + propionyl phosphate
show the reaction diagram		Clostridium acetobutylicum--642189-642189r
ATP + propionateADP + propionyl phosphate
show the reaction diagram		Methanosarcina thermophila--642184-642184r
ATP + propionateADP + propionyl phosphate
show the reaction diagram		Methanosarcina thermophila--659065, 659856--r
ATP + propionateADP + propionyl phosphate
show the reaction diagram		Thermotoga maritima--487537-487537r
ATP + propionateADP + propionyl phosphate
show the reaction diagram		Spirochaeta sp.--642179-642179r
ATP + propionateADP + propionyl phosphate
show the reaction diagram		Clostridium acetobutylicum-40% lower kinase activity than acetate642187-642187r
ATP + propionateADP + propionyl phosphate
show the reaction diagram		Geobacillus stearothermophilus-poor acceptor, 5% relative to acetate at 500 mM642180-642180r
ATP + propionateADP + propionyl phosphate
show the reaction diagram		Escherichia coli-one-tenth of the rate shown with acetate642166-642166r
CTP + acetateCDP + acetyl phosphate
show the reaction diagram		Geobacillus stearothermophilus--642172-642172r
CTP + acetateCDP + acetyl phosphate
show the reaction diagram		Rhodopseudomonas palustris--642183---
CTP + acetateCDP + acetyl phosphate
show the reaction diagram		Acholeplasma laidlawii--642176-642176r
CTP + acetateCDP + acetyl phosphate
show the reaction diagram		Clostridium acetobutylicum--642189-642189r
CTP + acetateCDP + acetyl phosphate
show the reaction diagram		Methanosarcina thermophila--642184-642184r
CTP + acetateCDP + acetyl phosphate
show the reaction diagram		Thermotoga maritima--487537-487537r
CTP + acetateCDP + acetyl phosphate
show the reaction diagram		Spirochaeta sp.--642179-642179r
GTP + acetateGDP + acetyl phosphate
show the reaction diagram		Escherichia coli--642182-642182r
GTP + acetateGDP + acetyl phosphate
show the reaction diagram		Geobacillus stearothermophilus--642172-642172r
GTP + acetateGDP + acetyl phosphate
show the reaction diagram		Geobacillus stearothermophilus--642180-642180r
GTP + acetateGDP + acetyl phosphate
show the reaction diagram		Rhodopseudomonas palustris--642183---
GTP + acetateGDP + acetyl phosphate
show the reaction diagram		Veillonella parvula--642173-642173r
GTP + acetateGDP + acetyl phosphate
show the reaction diagram		Veillonella parvula--642178-642178r
GTP + acetateGDP + acetyl phosphate
show the reaction diagram		Acholeplasma laidlawii--642176-642176r
GTP + acetateGDP + acetyl phosphate
show the reaction diagram		Propionibacterium freudenreichii--642181-642181r
GTP + acetateGDP + acetyl phosphate
show the reaction diagram		Clostridium acetobutylicum--642189-642189r
GTP + acetateGDP + acetyl phosphate
show the reaction diagram		Methanosarcina thermophila--642184-642184r
GTP + acetateGDP + acetyl phosphate
show the reaction diagram		Thermotoga maritima--487537-487537r
GTP + acetateGDP + acetyl phosphate
show the reaction diagram		Spirochaeta sp.--642179-642179r
ITP + acetateIDP + acetyl phosphate
show the reaction diagram		Salmonella enterica subsp. enterica serovar Typhimurium--642182-642182r
ITP + acetateIDP + acetyl phosphate
show the reaction diagram		Escherichia coli--642166-642166r
ITP + acetateIDP + acetyl phosphate
show the reaction diagram		Escherichia coli--642182-642182r
ITP + acetateIDP + acetyl phosphate
show the reaction diagram		Rhodopseudomonas palustris--642183---
ITP + acetateIDP + acetyl phosphate
show the reaction diagram		Veillonella parvula--642173-642173r
ITP + acetateIDP + acetyl phosphate
show the reaction diagram		Veillonella parvula--642178-642178r
ITP + acetateIDP + acetyl phosphate
show the reaction diagram		Acholeplasma laidlawii--642176-642176r
ITP + acetateIDP + acetyl phosphate
show the reaction diagram		Clostridium acetobutylicum--642189-642189r
ITP + acetateIDP + acetyl phosphate
show the reaction diagram		Methanosarcina thermophila--642184-642184r
ITP + acetateIDP + acetyl phosphate
show the reaction diagram		Thermotoga maritima--487537-487537r
TTP + acetateTDP + acetyl phosphate
show the reaction diagram		Veillonella parvula--642178-642178r
TTP + acetateTDP + acetyl phosphate
show the reaction diagram		Methanosarcina thermophila--642184-642184r
UTP + acetateUDP + acetyl phosphate
show the reaction diagram		Geobacillus stearothermophilus--642172-642172r
UTP + acetateUDP + acetyl phosphate
show the reaction diagram		Rhodopseudomonas palustris--642183---
UTP + acetateUDP + acetyl phosphate
show the reaction diagram		Propionibacterium freudenreichii--642181-642181r
UTP + acetateUDP + acetyl phosphate
show the reaction diagram		Clostridium acetobutylicum--642189-642189r
UTP + acetateUDP + acetyl phosphate
show the reaction diagram		Thermotoga maritima--487537-487537r
UTP + acetateUDP + acetyl phosphate
show the reaction diagram		Spirochaeta sp.--642179-642179r
ITP + acetateIDP + acetyl phosphate
show the reaction diagram		Spirochaeta sp.--642179-642179r
additional information?-Salmonella enterica subsp. enterica serovar Typhimurium, Escherichia coli-neither propionic acid nor butyric acid are active as substrates642182---
additional information?-Acholeplasma laidlawii-UTP is no phosphate donor, lactate, succinate, maleate formate and bicarbonate are not acceptors642176---
additional information?-Thermotoga maritima-butyrate, isobutyrate, valerate and isovalerate are no substrates487537---
additional information?-Spirochaeta sp.-isobutyrate, isovalerate, 2-methylbutyrate, butyrate and valerate are no substrates642179---
additional information?-Clostridium acetobutylicum-formate and butyrate are no substrates642189---
additional information?-Geobacillus stearothermophilus-UTP and CTP are active only partially642180---
additional information?-Geobacillus stearothermophilus-formate is no substrate642172---
additional information?-Methanosarcina thermophila-formate is no substrate642184---
additional information?-Propionibacterium freudenreichii-not active toward formate, butyrate, valerate and succinate642181---
additional information?-Veillonella parvula-enzyme does not react with malate, maleate, bicarbonate, butyrate, tartrate, citrate, formate or succinate642173---
additional information?-Escherichia coli-formate, butyrate and various holo and hydroxy derivatives of acetate and propionate are not phosphorylated642166---
additional information?-Clostridium acetobutylicum-butyrate is no substrate642187---

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Salmonella enterica subsp. enterica serovar Typhimurium--642182-642182
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Escherichia coli--642167-642167
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Escherichia coli--642177-642177
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Escherichia coli--642182-642182
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Geobacillus stearothermophilus--642172-642172
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Geobacillus stearothermophilus--642198-642198
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Rhodopseudomonas palustris--642183-642183
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Acinetobacter calcoaceticus--642185-642185
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Streptococcus pyogenes--642166-642166
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Moorella thermoacetica--642170-642170
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Veillonella parvula--642171-642171
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Veillonella parvula--642173-642173
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Veillonella parvula--642174-642174
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Veillonella parvula--642175-642175
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Veillonella parvula--642178-642178
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Lactobacillus delbrueckii--642166-642166
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Acholeplasma laidlawii--642176-642176
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Propionibacterium freudenreichii--642181-642181
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Clostridium acetobutylicum--642189-642189
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Desulfovibrio vulgaris--642186-642186
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Desulfovibrio vulgaris--642197-642197
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Methanosarcina thermophila--642191-642191
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Methanosarcina thermophila--642194-642194
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Methanosarcina thermophila--642196-642196
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Thermotoga maritima--487537-487537
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Clostridium thermocellum--642190-642190
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Spirochaeta sp.--642179-642179
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Methanosarcina thermophila--690888--
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Thermotoga maritimaQ9WYB1-691204--
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Mycoplasma pneumoniaeP75245-693619--
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Geobacillus stearothermophilus-function in the metabolism of pyruvate or synthesis of acetyl-CoA coupling with phosphoacetyltransacetylase642180-642180
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Escherichia coli-acetate kinase/phosphotransacetylase, major role of this two-enzyme sequence is to provide acetyl coenzyme A which may participate in fatty acid synthesis, citrate formation and subsequent oxidation642166-642166
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Lactobacillus sanfranciscensisQ9F1X7key enzyme and responsible for dephosphorylation of acetyl phosphate with the concomitant production of acetate and ATP642195-642195
ATP + acetateADP + acetyl phosphate
show the reaction diagram		Methanosarcina thermophila-function in the initial activation of acetate for conversion to methane and CO2642184-642184

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
ATPPropionibacterium acidipropioniciQ5BM21-672708 2D-image
ATPEthanoligenens harbinenseQ2N1I6-673209 2D-image
ATPRoseovarius sp.--673750 2D-image
ATPMethanosarcina thermophila--677237, 690888 2D-image
ATPAjellomyces capsulatus, Aspergillus fumigatus, Bacillus subtilis, Borrelia burgdorferi, Chaetomium globosum, Chlamydomonas reinhardtii, Coccidioides immitis, Coprinopsis cinerea, Cryptococcus neoformans, Cupriavidus necator, Emericella nidulans, Entamoeba histolytica, Enterococcus faecalis, Gibberella zeae, Helicobacter pylori, Hypocrea jecorina, Lactobacillus acidophilus, Lactococcus lactis, Listeria monocytogenes, Magnaporthe grisea, Mesoplasma florum, Methanosarcina acetivorans, Methanosarcina mazei, Mycoplasma pneumoniae, Neurospora crassa, Oceanobacillus iheyensis, Phaeosphaeria nodorum, Phanerochaete chrysosporium, Phytophthora ramorum, Phytophthora sojae, Salmonella enterica, Sclerotinia sclerotiorum, Staphylococcus aureus, Streptococcus pneumoniae, Uncinocarpus reesii, Ustilago maydis--677237 2D-image

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
Ca2+Thermotoga maritima--487537
Ca2+Geobacillus stearothermophilus--642172
Ca2+Veillonella parvula--642173
Ca2+Veillonella parvula-can replace Mg2+, but less effectively642178
Ca2+Spirochaeta sp.-isoenzyme I642179
Ca2+Propionibacterium freudenreichii--642181
Ca2+Methanosarcina thermophila--642184, 642196
Ca2+Clostridium acetobutylicum--642189
Co2+Thermotoga maritima--487537
Co2+Geobacillus stearothermophilus--642172
Co2+Veillonella parvula-can replace Mg2+642173, 642178
Co2+Spirochaeta sp.--642179
Co2+Propionibacterium freudenreichii--642181
Co2+Methanosarcina thermophila--642184
Co2+Clostridium acetobutylicum--642189
Cu2+Spirochaeta sp.--642179
Mg2+Thermotoga maritima--487537
Mg2+Escherichia coli-absolute requirement at pH 7.4642166
Mg2+Escherichia coli--642167, 642168, 642169
Mg2+Geobacillus stearothermophilus--642172, 642180
Mg2+Veillonella parvula--642173, 642178
Mg2+Acholeplasma laidlawii--642176
Mg2+Spirochaeta sp.--642179
Mg2+Propionibacterium freudenreichii--642181
Mg2+Rhodopseudomonas palustris-absolute requirement642183
Mg2+Clostridium acetobutylicum-strict requirement642187
Mg2+Clostridium thermocellum--642190
Mg2+Lactobacillus sanfranciscensisQ9F1X7-642195
Mg2+Methanosarcina thermophila--642196
Mg2+Desulfovibrio vulgaris--642197
Mg2+Methanosarcina thermophila-binding constants of enzyme variants with ADP measured690888
Mn2+Thermotoga maritima--487537
Mn2+Geobacillus stearothermophilus--642172, 642180
Mn2+Veillonella parvula--642173
Mn2+Spirochaeta sp.--642179
Mn2+Propionibacterium freudenreichii--642181
Mn2+Rhodopseudomonas palustris--642183
Mn2+Methanosarcina thermophila--642184, 642196
Mn2+Clostridium acetobutylicum--642187, 642189
Mn2+Clostridium thermocellum--642190
Ni2+Thermotoga maritima--487537
Ni2+Geobacillus stearothermophilus--642172
Pb2+Veillonella parvula--642173
Zn2+Thermotoga maritima--487537
Zn2+Geobacillus stearothermophilus--642172
Zn2+Spirochaeta sp.--642179
Zn2+Propionibacterium freudenreichii--642181
Mn2+Lactobacillus sanfranciscensisQ9F1X7-642195
additional informationGeobacillus stearothermophilus-Co2+, Ca2+, Cd2+ and Zn2+ can replace Mg2+ or Mn2+ only partially642180
additional informationPropionibacterium freudenreichii-no activity with Cu2+, Ni2+, Hg2+, Ba2+ or Fe2+642181
additional informationMethanosarcina thermophila-no activity with Cu2+, Ni2+ or Zn2+642184
additional informationClostridium acetobutylicum-no activity with Zn2+642189

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
2,4,6-Trinitrobenzene sulfonic acidGeobacillus stearothermophilus--642172 2D-image
5,5'-dithiobis(2-nitrobenzoic acid)Veillonella parvula--642174 2D-image
acetyl phosphateEscherichia coli-product inhibition is noncompetitive versus both acetate and ATP642168 2D-image
acetyl phosphateVeillonella parvula--642173 2D-image
acetyl phosphateAcholeplasma laidlawii--642176 2D-image
acetyl phosphateDesulfovibrio vulgaris-product inhibition642197 2D-image
adenosine 5'-(gamma-thio)triphosphateMethanosarcina thermophila--659446 2D-image
ADPVeillonella parvula--642173 2D-image
ADPAcholeplasma laidlawii--642176 2D-image
BromoacetateVeillonella parvula--642175 2D-image
CrATPEscherichia coli--642168 2D-image
CrGTPEscherichia coli--642169 2D-image
DiethyldicarbonateAcinetobacter calcoaceticus--642185 2D-image
DiethyldicarbonateMethanosarcina thermophila--642193 2D-image
HgCl2Escherichia coli--642166, 642177 2D-image
HgCl2Veillonella parvula--642173 2D-image
HgCl2Acholeplasma laidlawii--642176 2D-image
HgCl2Clostridium acetobutylicum--642189 2D-image
hydroxylamineThermotoga maritimaQ9WYB1inhibits acetate kinase reaction in a nonlinear and noncompetitive fashion, substantial inhibition at concentrations of 704 mM and minimal inhibition at concentrations of 250 microM hydroxylamine691204 2D-image
iodoacetamideVeillonella parvula--642173, 642174 2D-image
iodoacetateVeillonella parvula--642174, 642175 2D-image
IodosobenzoateEscherichia coli--642166 2D-image
K+Acholeplasma laidlawii--642176 2D-image
N-ethylmaleimideVeillonella parvula--642173, 642174 2D-image
N-ethylmaleimideEscherichia coli--642177 2D-image
N-ethylmaleimideMethanosarcina thermophila--642191 2D-image
Na+Acholeplasma laidlawii--642176 2D-image
Na+Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium--642182 2D-image
p-chloromercuribenzoateVeillonella parvula--642173 2D-image
p-chloromercuribenzoateEscherichia coli--642177 2D-image
p-hydroxymercuribenzoateClostridium acetobutylicum--642189 2D-image
p-mercuribenzoateEscherichia coli--642166 2D-image
p-mercuribenzoateVeillonella parvula--642174 2D-image
PhenylglyoxalMethanosarcina thermophila--642194 2D-image
phosphateVeillonella parvula--642173 2D-image
potassium hydroxylamineVeillonella parvula--642173 2D-image
Propionic acidEscherichia coli-competitive inhibitor with respect to acetate642182 2D-image
Propionic acidSalmonella enterica subsp. enterica serovar Typhimurium--642182 2D-image
sodium hydroxylamineVeillonella parvula--642173 2D-image
Li+Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium--642182 2D-image
additional informationGeobacillus stearothermophilus-not inactivated by 5,5'-dithiobis(2-nitrobenzoic acid), tetranitromethane or 2-hydroxy-3-nitro-benzyl bromide642172-
additional informationAcholeplasma laidlawii-not inactivated by N-ethylmaleimide642176-
additional informationAcinetobacter calcoaceticus-5,5'-dithiobis(2-nitrobenzoic acid), p-chloromercuriphenylsulfonate, N-ethylmaleimide and phenylglyoxal does not affect the enzyme activity642185-
additional informationClostridium acetobutylicum-iodoacetate and iodoacetamide does not inhibit642187-
additional informationMethanosarcina thermophila-not inactivated by N-ethylmaleimide642191-

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
alpha-glycerophosphateGeobacillus stearothermophilus--642180 2D-image
D-fructose 1,6-bisphosphateGeobacillus stearothermophilus-activates formation of ADP642180 2D-image
succinateVeillonella parvula-heterotropic allosteric effector, necessary for enzymatic activity in both directions642171 2D-image
succinateVeillonella parvula--642174, 642175 2D-image
succinateVeillonella parvula-heterotropically stimulated by642178 2D-image
D-Glucose 1,6-bisphosphateGeobacillus stearothermophilus--642180 2D-image
additional informationVeillonella parvula-succinate is unnecessary for the activity of this enzyme642173-

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
1.5-acetateMethanosarcina thermophila-wild-type, pH 7.0659065 2D-image
2.3-acetateMethanosarcina thermophila-pH 7.0, 20°C, mutant E97A, produced in E. coli642191 2D-image
2.3-acetateMethanosarcina thermophila--642194 2D-image
2.7-acetateMethanosarcina thermophila-wild-type, pH 7.0659446 2D-image
2.7-acetateMethanosarcina thermophila-wild-type690888 2D-image
2.8-acetateMethanosarcina thermophila-mutant E97D, pH 7.5, 37°C659856 2D-image
4-acetateMethanosarcina thermophila-pH 7.0, 20°C, mutant E97Q, produced in E. coli642191 2D-image
4-acetateMethanosarcina thermophila-pH 7.6, 37°C, mutant R175K642194 2D-image
5.8-acetateMethanosarcina thermophila-R241A/Q43W, double mutant690888 2D-image
7-acetateEscherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium-pH 7.9, 37°C642182 2D-image
7.2-acetateMethanosarcina thermophila-pH 7.0, 20°C, mutant E97D, produced in E. coli642191 2D-image
7.2-acetateMethanosarcina thermophila--642194 2D-image
10-acetateMethanosarcina thermophila-pH 7.0, 37°C, mutant H180R, produced in E. coli642193 2D-image
10.4-acetateMethanosarcina thermophila-mutant F179A, pH 7.0659065 2D-image
11.7-acetateSpirochaeta sp.-pH 7.5, 30°C, isoenzyme I642179 2D-image
12.4-acetateMethanosarcina thermophila-Q43W, mutant690888 2D-image
13-acetateMethanosarcina thermophila-pH 7.0, 37°C, mutant H180D, produced in E. coli642193 2D-image
14-acetateMethanosarcina thermophila-pH 7.0, 37°C, mutant H180Q, produced in E. coli642193 2D-image
15-acetateMethanosarcina thermophila-pH 7.0, 20°C, mutant E334A, produced in E. coli642191 2D-image
15-acetateMethanosarcina thermophila-pH 7.0, 37°C, mutant H184A, produced in E. coli642193 2D-image
16-acetateMethanosarcina thermophila-pH 7.0, 37°C, mutant H123A, produced in E. coli642193 2D-image
17-acetateMethanosarcina thermophila-pH 7.0, 20°C, mutant E385C, produced in E. coli642191 2D-image
17-acetateMethanosarcina thermophila-pH 7.0, 37°C, mutant H180E, H180N, produced in E. coli642193 2D-image
18-acetateMethanosarcina thermophila-pH 7.0, 37°C, unaltered wild-type enzyme, produced in E. coli642193 2D-image
19-acetateMethanosarcina thermophila-pH 7.0, 20°C, unaltered enzyme, produced in E. coli642191 2D-image
19-acetateMethanosarcina thermophila-pH 7.0, 37°C, mutant H180K, produced in E. coli642193 2D-image
19-acetateMethanosarcina thermophila-pH 7.6, 37°C642194 2D-image
19.5-acetateMethanosarcina thermophila-mutant L122A, pH 7.0659065 2D-image
20-acetateMethanosarcina thermophila-pH 7.0, 37°C, mutant H60A, produced in E. coli642193 2D-image
20-acetateMethanosarcina thermophila-pH 7.6, 37°C, mutant R340L642194 2D-image
21.4-acetateMethanosarcina thermophila-mutant V93A, pH 7.0659065 2D-image
22-acetateMethanosarcina thermophila-pH 7.3, 37°C642184 2D-image
22-acetateMethanosarcina thermophila--642191 2D-image
22-acetateMethanosarcina thermophila-pH 7.0, 37°C, authentic wild-type enzyme642193 2D-image
22-acetateMethanosarcina thermophila-wild-type, pH 7.5, 37°C659856 2D-image
24-acetateMethanosarcina thermophila-wild-type, pH 7.0, presence of 200 mM guanidine/HCl659446 2D-image
26-acetateMethanosarcina thermophila-pH 7.0, 20°C, mutant E32A, produced in E. coli642191 2D-image
30-acetateVeillonella parvula-pH 7.4, 37°C642173 2D-image
30-acetateMethanosarcina thermophila-mutant R241A, pH 7.0, presence of 200 mM guanidine/HCl659446 2D-image
32-acetateMethanosarcina thermophila-mutant P232A, pH 7.0659065 2D-image
33-acetateMethanosarcina thermophila-pH 7.0, 37°C, mutant H208A, produced in E. coli642193 2D-image
38.5-acetateAcholeplasma laidlawii-pH 7.4, 25°C642176 2D-image
38.5-acetateSpirochaeta sp.-pH 7.5, 30°C, isoenzyme II642179 2D-image
39.5-acetateMethanosarcina thermophila-mutant V93G, pH 7.0659065 2D-image
40-acetateThermotoga maritima-pH 7.0, 50°C487537 2D-image
40-acetateRhodopseudomonas palustris-carried out at various temperatures642183 2D-image
42-acetateMethanosarcina thermophila-pH 7.6, 37°C, mutant R340K642194 2D-image
44-acetateMethanosarcina thermophila-pH 7.0, 20°C, mutant E385Q, produced in E. coli642191 2D-image
48-acetateLactobacillus sanfranciscensisQ9F1X7pH 7.5, 30°C642195 2D-image
48-acetateMethanosarcina thermophila-pH 7.4, 37°C, wild-type642196 2D-image
48-acetateMethanosarcina thermophila-R91K/Q43W, double mutant690888 2D-image
66-acetateMethanosarcina thermophila-pH 7.0, 37°C, mutant H94A, produced in E. coli642193 2D-image
66-acetateMethanosarcina thermophila--642196 2D-image
70-acetateMethanosarcina thermophila-mutant R91K, pH 7.0659446 2D-image
73-acetateClostridium acetobutylicum-pH 7.3, 30°C642189 2D-image
77-acetateMethanosarcina thermophila-mutant R241K, pH 7.0659446 2D-image
80.9-acetatePropionibacterium freudenreichii-pH 7.5, 30°C642181 2D-image
83-acetateMethanosarcina thermophila-mutant R91A, pH 7.0, presence of 200 mM guanidine/HCl659446 2D-image
86-acetateMethanosarcina thermophila-pH 7.0, 20°C, mutant E385A, produced in E. coli642191 2D-image
90-acetateStreptococcus pyogenes-pH 7.4642166 2D-image
100-acetateVeillonella parvula-pH 7.3, 37°C, 50 M succinate642171 2D-image
111-acetateMethanosarcina thermophila-pH 7.4, 37°C, mutant N211A642196 2D-image
129-acetateMethanosarcina thermophila-pH 7.6, 37°C, mutant R285L642194 2D-image
135-acetateMoorella thermoacetica--642170 2D-image
135-acetateClostridium thermocellum-pH 7.2-8.0, 50°C642190 2D-image
160-acetateClostridium acetobutylicum-pH 7.0, 36°C642187 2D-image
170-acetateVeillonella parvula-pH 7.3, 37°C, 5.0 M succinate642171 2D-image
170-acetateMethanosarcina thermophila-pH 7.4, 37°C, mutant D148A642196 2D-image
179-acetateMethanosarcina thermophila-R241K/Q43W, double mutant690888 2D-image
200-acetateMethanosarcina thermophila-pH 7.4, 37°C, mutant S11A642196 2D-image
206-acetateMethanosarcina thermophila-pH 7.6, 37°C, mutant R285A642194 2D-image
219-acetateMethanosarcina thermophila-pH 7.6, 37°C, mutant R285K642194 2D-image
239-acetateMethanosarcina thermophila-pH 7.4, 37°C, mutant K14R642196 2D-image
250-acetateMethanosarcina thermophila-mutant R91A, pH 7.0659446 2D-image
260-acetateMethanosarcina thermophila-pH 7.6, 37°C, mutant R91K642194 2D-image
266-acetateMethanosarcina thermophila-pH 7.4, 37°C, mutant S10T642196 2D-image
270-acetateMethanosarcina thermophila-mutant R241L, pH 7.0659446 2D-image
300-acetateEscherichia coli-pH 7.4642166 2D-image
330-acetateMethanosarcina thermophila-pH 7.0, 37°C, mutant H90A, produced in E. coli642193 2D-image
333-acetateMethanosarcina thermophila-pH 7.4, 37°C, mutant S12T642196 2D-image
337-acetateMethanosarcina thermophila-pH 7.0, 20°C, mutant E385D, produced in E. coli642191 2D-image
390-acetateMethanosarcina thermophila-pH 7.4, 37°C, mutant D148N642196 2D-image
420-acetateMethanosarcina thermophila-mutant R91L, pH 7.0659446 2D-image
693-acetateMethanosarcina thermophila-pH 7.4, 37°C, mutant N7D642196 2D-image
710-acetateMethanosarcina thermophila-mutant R241A, pH 7.0659446 2D-image
800-acetateVeillonella parvula-pH 7.3, 37°C642175 2D-image
814-acetateMethanosarcina thermophila-pH 7.4, 37°C, mutant S10A642196 2D-image
1573-acetateMethanosarcina thermophila-pH 7.4, 37°C, mutant S11T642196 2D-image
0.0026-acetyl phosphateRhodopseudomonas palustris-carried out at various temperatures642183 2D-image
0.1-acetyl phosphateAcholeplasma laidlawii-pH 7.4, 25°C642176 2D-image
0.15-acetyl phosphateMethanosarcina thermophila-mutant F179A, pH 7.4659065 2D-image
0.16-acetyl phosphateEscherichia coli-pH 7.9, 37°C642182 2D-image
0.16-acetyl phosphateSalmonella enterica subsp. enterica serovar Typhimurium--642182 2D-image
0.2-acetyl phosphateAcholeplasma laidlawii-pH 7.4, 25°C, 0.5 M NaCl642176 2D-image
0.26-acetyl phosphateMethanosarcina thermophila-Q43W690888 2D-image
0.33-acetyl phosphateMethanosarcina thermophila-R241/Q43W690888 2D-image
0.34-acetyl phosphateMethanosarcina thermophila-wild-type, pH 7.4659065 2D-image
0.44-acetyl phosphateThermotoga maritima-pH 7.0, 50°C487537 2D-image
0.44-acetyl phosphatePropionibacterium freudenreichii-pH 7.5, 30°C642181 2D-image
0.47-acetyl phosphateMethanosarcina thermophila-wild-type, pH 7.4659446 2D-image
0.47-acetyl phosphateMethanosarcina thermophila-wild-type690888 2D-image
0.5-acetyl phosphateMethanosarcina thermophila-mutant L122A, pH 7.4659065 2D-image
0.58-acetyl phosphateClostridium acetobutylicum-pH 7.3, 30°C642189 2D-image
0.61-acetyl phosphateMethanosarcina thermophila-mutant R91K, pH 7.4659446 2D-image
0.71-acetyl phosphateMethanosarcina thermophila-mutant V93G, pH 7.4659065 2D-image
0.74-acetyl phosphateMethanosarcina thermophila-mutant V93A, pH 7.4659065 2D-image
0.84-acetyl phosphateMethanosarcina thermophila-mutant R241A, pH 7.4659446 2D-image
0.92-acetyl phosphateMethanosarcina thermophila-mutant R241K, pH 7.4659446 2D-image
1-acetyl phosphateLactobacillus sanfranciscensisQ9F1X7pH 7.5, 30°C642195 2D-image
1.02-acetyl phosphateMethanosarcina thermophila-R241K/Q43W690888 2D-image
1.08-acetyl phosphateMethanosarcina thermophila-R91K/Q43W690888 2D-image
1.19-acetyl phosphateMethanosarcina thermophila-mutant P232A, pH 7.4659065 2D-image
1.2-acetyl phosphateVeillonella parvula-pH 7.4, 37°C642173 2D-image
1.36-acetyl phosphateMethanosarcina thermophila-mutant R91A, pH 7.4659446 2D-image
1.64-acetyl phosphateMethanosarcina thermophila-mutant R241L, pH 7.4659446 2D-image
2.3-acetyl phosphateMethanosarcina thermophila-mutant R91L, pH 7.4659446 2D-image
2.58-acetyl phosphateMethanosarcina thermophila-R91A/Q43W690888 2D-image
3.3-acetyl phosphateVeillonella parvula-pH 7.3, 37°C, 2.0 M succinate642171 2D-image
5-acetyl phosphateEscherichia coli-pH 7.4642166 2D-image
5-acetyl phosphateVeillonella parvula-pH 7.3, 37°C, 0.2 M succinate642171 2D-image
7-acetyl phosphateVeillonella parvula-pH 7.3, 37°C642175 2D-image
0.063-ADPMethanosarcina thermophila-mutant R91A, pH 7.4; mutant R91L, pH 7.4659446 2D-image
0.087-ADPRhodopseudomonas palustris-carried out at various temperatures642183 2D-image
0.098-ADPMethanosarcina thermophila-wild-type, pH 7.4659446 2D-image
0.098-ADPMethanosarcina thermophila-wild-type690888 2D-image
0.147-ADPMethanosarcina thermophila-R91A/Q43W, double mutant690888 2D-image
0.164-ADPMethanosarcina thermophila-R91K/Q43W, double mutant690888 2D-image
0.168-ADPMethanosarcina thermophila-mutant R91K, pH 7.4659446 2D-image
0.173-ADPMethanosarcina thermophila-Q43W, mutant690888 2D-image
0.2-ADPPropionibacterium freudenreichii-pH 7.5, 30°C642181 2D-image
0.21-ADPMethanosarcina thermophila-mutant R241A, pH 7.4659446 2D-image
0.239-ADPMethanosarcina thermophila-R241A/Q43W, double mutant690888 2D-image
0.24-ADPAcholeplasma laidlawii-pH 7.4, 25°C642176 2D-image
0.3-ADPVeillonella parvula-pH 7.4, 37°C642173 2D-image
0.3-ADPLactobacillus sanfranciscensisQ9F1X7pH 7.5, 30°C642195 2D-image
0.5-ADPEscherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium-pH 7.9, 37°C642182 2D-image
0.578-ADPMethanosarcina thermophila-mutant R241K, pH 7.4659446 2D-image
0.692-ADPMethanosarcina thermophila-R241K/Q43W690888 2D-image
0.71-ADPClostridium acetobutylicum-pH 7.3, 30°C642189 2D-image
1.1-ADPVeillonella parvula-pH 7.3, 37°C, 1.0 M succinate642171 2D-image
1.12-ADPDesulfovibrio vulgaris-pH 7.5, 25°C642197 2D-image
1.26-ADPMethanosarcina thermophila-mutant R241L, pH 7.4659446 2D-image
1.3-ADPVeillonella parvula-pH 7.3, 37°C, 0.2 M succinate642171 2D-image
1.3-ADPVeillonella parvula-pH 7.3, 37°C642175 2D-image
1.5-ADPEscherichia coli-pH 7.4642166 2D-image
1.53-ADPAcholeplasma laidlawii-pH 7.4, 25°C, 0.5 M NaCl642176 2D-image
3-ADPThermotoga maritima-pH 7.0, 50°C487537 2D-image
6-ADPClostridium acetobutylicum-pH 7.0, 36°C642187 2D-image
0.016-ATPMethanosarcina thermophila-mutant R91A, pH 7.0659446 2D-image
0.0499-ATPMethanosarcina thermophila-mutant V93A, pH 7.0659065 2D-image
0.0506-ATPMethanosarcina thermophila-mutant F179A, pH 7.0659065 2D-image
0.053-ATPMethanosarcina thermophila-Q43W, mutant690888 2D-image
0.0542-ATPMethanosarcina thermophila-mutant P232A, pH 7.0659065 2D-image
0.0576-ATPMethanosarcina thermophila-mutant L122A, pH 7.0659065 2D-image
0.068-ATPMethanosarcina thermophila-mutant R91A, pH 7.0, presence of 200 mM guanidine/HCl; wild-type, pH 7.0, presence of 200 mM guanidine/HCl659446 2D-image
0.0687-ATPMethanosarcina thermophila-mutant V93G, pH 7.0659065 2D-image
0.07-ATPEscherichia coli-pH 7.9, 37°C642182 2D-image
0.07-ATPSalmonella enterica subsp. enterica serovar Typhimurium--642182 2D-image
0.0713-ATPMethanosarcina thermophila-wild-type, pH 7.0659065 2D-image
0.076-ATPMethanosarcina thermophila-mutant R91K, pH 7.0659446 2D-image
0.08-ATPMethanosarcina thermophila-wild-type, pH 7.0659446 2D-image
0.08-ATPMethanosarcina thermophila-R241A/Q43W, double mutant; wild-type690888 2D-image
0.145-ATPMethanosarcina thermophila-mutant R91L, pH 7.0659446 2D-image
0.22-ATPPropionibacterium freudenreichii-pH 7.5, 30°C642181 2D-image
0.234-ATPMethanosarcina thermophila-R91K/Q43W, double mutant690888 2D-image
0.264-ATPMethanosarcina thermophila-mutant R241A, pH 7.0, presence of 200 mM guanidine/HCl659446 2D-image
0.297-ATPMethanosarcina thermophila-mutant R241A, pH 7.0659446 2D-image
0.3-ATPAcholeplasma laidlawii-pH 7.4, 25°C642176 2D-image
0.37-ATPClostridium acetobutylicum-pH 7.3, 30°C642189 2D-image
0.539-ATPMethanosarcina thermophila-R91A/Q43W, double mutant690888 2D-image
0.6-ATPLactobacillus sanfranciscensisQ9F1X7pH 7.5, 30°C642195 2D-image
0.7-ATPThermotoga maritima-pH 7.0, 50°C487537 2D-image
0.7-ATPMethanosarcina thermophila-pH 7.0, 20°C, mutant E334A, produced in E. coli642191 2D-image
0.7-ATPMethanosarcina thermophila-pH 7.0, 37°C, mutant H180A, produced in E. coli642193 2D-image
0.9-ATPMethanosarcina thermophila-pH 7.6, 37°C, mutant R340L642194 2D-image
0.94-ATPEscherichia coli-pH 7.9, 37°C642182 2D-image
1-ATPMethanosarcina thermophila-pH 7.0, 20°C, unaltered enzyme, produced in E. coli642191 2D-image
1-ATPMethanosarcina thermophila-pH 7.6, 37°C, wild-type and mutant R175K642194 2D-image
1.1-ATPRhodopseudomonas palustris-carried out at various temperatures642183 2D-image
1.1-ATPMethanosarcina thermophila-pH 7.0, 20°C, mutant E32A, produced in E. coli642191 2D-image
1.3-ATPMethanosarcina thermophila-pH 7.6, 37°C, mutant R340K642194 2D-image
1.3-ATPMethanosarcina thermophila-R241K/Q43W, double mutant690888 2D-image
1.4-ATPMethanosarcina thermophila-pH 7.0, 20°C, mutants E97A, E97D, E97Q, produced in E. coli642191 2D-image
1.4-ATPMethanosarcina thermophila-pH 7.0, 37°C, mutant H180R, produced in E. coli642193 2D-image
1.4-ATPMethanosarcina thermophila--642194 2D-image
1.5-ATPMethanosarcina thermophila-pH 7.0, 37°C, mutant H123A, produced in E. coli642193 2D-image
1.5-ATPMethanosarcina thermophila--642196 2D-image
1.6-ATPMethanosarcina thermophila-pH 7.0, 20°C, mutant E385C, produced in E. coli642191 2D-image
1.7-ATPMethanosarcina thermophila-pH 7.0, 37°C, mutant H90A, produced in E. coli642193 2D-image
1.9-ATPMethanosarcina thermophila-pH 7.0, 37°C, mutant H208A, produced in E. coli642193 2D-image
2-ATPVeillonella parvula-pH 7.4, 37°C642173 2D-image
2-ATPMethanosarcina thermophila-pH 7.0, 20°C, mutant E385Q, produced in E. coli642191 2D-image
2-ATPMethanosarcina thermophila-pH 7.0, 37°C, unaltered wild-type enzyme, produced in E. coli642193 2D-image
2.13-ATPMethanosarcina thermophila-pH 7.4, 37°C, wild-type642196 2D-image
2.5-ATPClostridium acetobutylicum-pH 7.0, 36°C642187 2D-image
2.7-ATPMethanosarcina thermophila-pH 7.0, 37°C, mutant H184A, produced in E. coli642193 2D-image
2.8-ATPMethanosarcina thermophila-pH 7.3, 37°C642184 2D-image
2.8-ATPMethanosarcina thermophila--642191 2D-image
2.8-ATPMethanosarcina thermophila-pH 7.0, 37°C, authentic wild-type enzyme642193 2D-image
2.8-ATPMethanosarcina thermophila-pH 7.6, 37°C, recombinant wild-type642194 2D-image
3-ATPMethanosarcina thermophila-pH 7.0, 37°C, mutant H60A, produced in E. coli642193 2D-image
3-ATPMethanosarcina thermophila-pH 7.6, 37°C, mutant R285K, R285L642194 2D-image
3-ATPMethanosarcina thermophila-pH 7.4, 37°C, mutant N211A642196 2D-image
3.1-ATPMethanosarcina thermophila-pH 7.4, 37°C, mutant D148A642196 2D-image
3.2-ATPMethanosarcina thermophila-pH 7.4, 37°C, mutant S10A642196 2D-image
3.4-ATPMethanosarcina thermophila-pH 7.6, 37°C, mutant R241K642194 2D-image
3.62-ATPMethanosarcina thermophila-pH 7.4, 37°C, mutant K14R642196 2D-image
3.7-ATPSpirochaeta sp.-pH 7.5, 30°C, isoenzyme II642179 2D-image
3.7-ATPMethanosarcina thermophila-pH 7.0, 37°C, mutant H180Q, produced in E. coli642193 2D-image
3.7-ATPMethanosarcina thermophila-pH 7.4, 37°C, mutant S12T642196 2D-image
3.8-ATPSpirochaeta sp.-pH 7.5, 30°C, isoenzyme I642179 2D-image
3.9-ATPMethanosarcina thermophila-pH 7.6, 37°C, mutant R91K642194 2D-image
4.3-ATPMethanosarcina thermophila-pH 7.0, 20°C, mutant E385D, produced in E. coli642191 2D-image
4.3-ATPMethanosarcina thermophila-pH 7.4, 37°C, mutant S10T642196 2D-image
4.5-ATPMethanosarcina thermophila--642191 2D-image
4.5-ATPMethanosarcina thermophila-pH 7.4, 37°C, mutant S11A642196 2D-image
4.6-ATPMethanosarcina thermophila-pH 7.0, 37°C, mutant H180N, produced in E. coli642193 2D-image
4.7-ATPMethanosarcina thermophila-pH 7.0, 37°C, mutant H180D, produced in E. coli642193 2D-image
5-ATPMethanosarcina thermophila-pH 7.4, 37°C, mutant N7D, D148E642196 2D-image
5.2-ATPMethanosarcina thermophila-pH 7.4, 37°C, mutant K14A642196 2D-image
5.8-ATPMethanosarcina thermophila-pH 7.0, 37°C, mutant H94A, produced in E. coli642193 2D-image
5.8-ATPMethanosarcina thermophila-pH 7.4, 37°C, mutant S10T642196 2D-image
6-ATPVeillonella parvula-pH 7.3, 37°C642175 2D-image
6-ATPMethanosarcina thermophila-pH 7.6, 37°C, mutant R285A, R91A642194 2D-image
6.2-ATPMethanosarcina thermophila-pH 7.0, 37°C, mutant H180E, produced in E. coli642193 2D-image
6.3-ATPMethanosarcina thermophila-pH 7.0, 37°C, mutant H180K, produced in E. coli642193 2D-image
6.8-ATPMethanosarcina thermophila-pH 7.4, 37°C, mutant S11T642196 2D-image
6.9-ATPMethanosarcina thermophila-pH 7.0, 20°C, mutant E385A, produced in E. coli642191 2D-image
7-ATPMethanosarcina thermophila-pH 7.4, 37°C, mutant N7A642196 2D-image
7.3-ATPMethanosarcina thermophila-pH 7.4, 37°C, mutant S12A642196 2D-image
7.7-ATPMethanosarcina thermophila-pH 7.4, 37°C, mutant E384A642196 2D-image
9-ATPVeillonella parvula-pH 7.3, 37°C, 50 M succinate642171 2D-image
10-ATPVeillonella parvula-pH 7.3, 37°C, 5.0 M succinate642171 2D-image
11.4-ATPMethanosarcina thermophila-mutant R241K, pH 7.0659446 2D-image
17-ATPMethanosarcina thermophila-mutant R241L, pH 7.0659446 2D-image
56-ATPMethanosarcina thermophila-pH 7.0, 37°C, mutant H180A, produced in E. coli642193 2D-image
33.4-ButyrateMethanosarcina thermophila-mutant V93A, pH 7.0659065 2D-image
39-ButyrateMethanosarcina thermophila-wild-type, pH 7.0659065 2D-image
63-ButyrateMethanosarcina thermophila-mutant V93G, pH 7.0659065 2D-image
200-ButyrateRhodopseudomonas palustris-carried out at various temperatures642183 2D-image
1.9-CTPRhodopseudomonas palustris-carried out at various temperatures642183 2D-image
1.1-GTPEscherichia coli-pH 7.9, 37°C642182 2D-image
5.7-GTPRhodopseudomonas palustris-carried out at various temperatures642183 2D-image
420-isobuytyrateRhodopseudomonas palustris-carried out at various temperatures642183 2D-image
0.78-ITPEscherichia coli-pH 7.9, 37°C642182 2D-image
6.2-PropionateMethanosarcina thermophila-mutant V93A, pH 7.0659065 2D-image
10.7-PropionateMethanosarcina thermophila-mutant L122A, pH 7.0659065 2D-image
11-PropionateMethanosarcina thermophila-mutant F179A, pH 7.0659065 2D-image
14.4-PropionateMethanosarcina thermophila-wild-type, pH 7.0659065 2D-image
24-PropionateMethanosarcina thermophila-wild-type, pH 7.5, 37°C659856 2D-image
25-PropionateMethanosarcina thermophila-mutant V93G, pH 7.0659065 2D-image
40-PropionateSpirochaeta sp.-pH 7.5, 30°C, isoenzyme I642179 2D-image
46-PropionateMethanosarcina thermophila-mutant P232A, pH 7.0659065 2D-image
63.3-PropionatePropionibacterium freudenreichii-pH 7.5, 30°C642181 2D-image
133-PropionateSpirochaeta sp.-pH 7.5, 30°C, isoenzyme II642179 2D-image
150-PropionateRhodopseudomonas palustris-carried out at various temperatures642183 2D-image
300-PropionateVeillonella parvula-pH 7.3, 37°C, 50 M succinate642171 2D-image
1000-PropionateVeillonella parvula-pH 7.3, 37°C, 5.0 M succinate642171 2D-image
0.14-Propionyl phosphatePropionibacterium freudenreichii-pH 7.5, 30°C642181 2D-image
1.3-UTPRhodopseudomonas palustris-carried out at various temperatures642183 2D-image
3.7-ITPRhodopseudomonas palustris-carried out at various temperatures642183 2D-image
additional information-additional informationMethanosarcina thermophila-assay methods for both directions of reaction659856-
additional information-additional informationThermotoga maritimaQ9WYB1lower Km of the Thermotoga maritima acetate kinase for acetate measured than that determined by earlier assay methods691204-

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
190-acetateMethanosarcina thermophila-mutant E97D, pH 7.5, 37°C659856 2D-image
280-acetateClostridium acetobutylicum-pH 7.0, 36°C642187 2D-image
1042-acetateMethanosarcina thermophila-wild-type, pH 7.5, 37°C659856 2D-image
12-acetyl phosphateMethanosarcina thermophila-mutant F179A, pH 7.4659065 2D-image
700-acetyl phosphateClostridium acetobutylicum-pH 7.0, 36°C642187 2D-image
875-acetyl phosphateAcholeplasma laidlawii-pH 7.4, 25°C642176 2D-image
1039-acetyl phosphateMethanosarcina thermophila-mutant L122A, pH 7.4659065 2D-image
2396-acetyl phosphateMethanosarcina thermophila-mutant P232A, pH 7.4659065 2D-image
2572-acetyl phosphateMethanosarcina thermophila-mutant V93G, pH 7.4659065 2D-image
2680-acetyl phosphateMethanosarcina thermophila-wild-type, pH 7.4659065 2D-image
3869-acetyl phosphateMethanosarcina thermophila-mutant V93A, pH 7.4659065 2D-image
0.42-ADPMethanosarcina thermophila-mutant R241L, pH 7.4659446 2D-image
2.63-ADPMethanosarcina thermophila-R91A/Q43W, double mutant690888 2D-image
3.5-ADPMethanosarcina thermophila-mutant R91A, pH 7.4659446 2D-image
4.5-ADPMethanosarcina thermophila-mutant R241A, pH 7.4; mutant R241K, pH 7.4659446 2D-image
7.7-ADPMethanosarcina thermophila-mutant R91L, pH 7.4659446 2D-image
10.5-ADPMethanosarcina thermophila-R241K/Q43W, double mutant690888 2D-image
14.6-ADPMethanosarcina thermophila-R241A/Q43W, double mutant690888 2D-image
23-ADPMethanosarcina thermophila-mutant R91K, pH 7.4659446 2D-image
25.7-ADPMethanosarcina thermophila-R91K/Q43W, double mutant690888 2D-image
177-ADPAcholeplasma laidlawii-pH 7.4, 25°C, 0.5 M NaCl642176 2D-image
318-ADPAcholeplasma laidlawii-pH 7.4, 25°C, 0.5 M NaCl642176 2D-image
532-ADPClostridium acetobutylicum-pH 7.0, 36°C642187 2D-image
1000-ADPAcholeplasma laidlawii-pH 7.4, 25°C642176 2D-image
1255-ADPMethanosarcina thermophila-wild-type690888 2D-image
1260-ADPMethanosarcina thermophila-wild-type, pH 7.4659446 2D-image
2650-ADPMethanosarcina thermophila-Q43W, mutant690888 2D-image
0.11-ATPMethanosarcina thermophila-mutant R91A, pH 7.0659446 2D-image
0.22-ATPMethanosarcina thermophila-mutant R91L, pH 7.0659446 2D-image
0.4-ATPMethanosarcina thermophila-R241K/Q43W, double mutant690888 2D-image
0.68-ATPMethanosarcina thermophila-mutant R241A, pH 7.0659446 2D-image
1.3-ATPMethanosarcina thermophila-mutant R241K, pH 7.0659446 2D-image
1.38-ATPMethanosarcina thermophila-mutant R241L, pH 7.0659446 2D-image
1.83-ATPMethanosarcina thermophila-R241A/Q43W, double mutant690888 2D-image
2.07-ATPMethanosarcina thermophila-R91A/Q43W, double mutant690888 2D-image
2.2-ATPMethanosarcina thermophila-mutant F179A, pH 7.0659065 2D-image
3.7-ATPMethanosarcina thermophila-mutant R91K, pH 7.0659446 2D-image
11.8-ATPMethanosarcina thermophila-R91K/Q43W, double mutant690888 2D-image
132-ATPMethanosarcina thermophila-mutant P232A, pH 7.0659065 2D-image
150-ATPMethanosarcina thermophila-mutant L122A, pH 7.0659065 2D-image
238-ATPClostridium acetobutylicum-pH 7.0, 36°C642187 2D-image
268-ATPAcholeplasma laidlawii-pH 7.4, 25°C642176 2D-image
347-ATPMethanosarcina thermophila-mutant V93G, pH 7.0659065 2D-image
699-ATPMethanosarcina thermophila-Q43W, mutant690888 2D-image
844-ATPMethanosarcina thermophila-mutant V93A, pH 7.0659065 2D-image
913-ATPMethanosarcina thermophila-wild-type, pH 7.0659446 2D-image
913-ATPMethanosarcina thermophila-wild-type690888 2D-image
1055-ATPMethanosarcina thermophila-wild-type, pH 7.0659065 2D-image
0.18-ButyrateMethanosarcina thermophila-wild-type, pH 7.0659065 2D-image
42.4-ButyrateMethanosarcina thermophila-mutant V93A, pH 7.0659065 2D-image
294-ButyrateMethanosarcina thermophila-mutant V93G, pH 7.0659065 2D-image
0.37-PropionateMethanosarcina thermophila-mutant F179A, pH 7.0659065 2D-image
6.7-PropionateMethanosarcina thermophila-mutant L122A, pH 7.0659065 2D-image
8.5-PropionateMethanosarcina thermophila-mutant P232A, pH 7.0659065 2D-image
218-PropionateMethanosarcina thermophila-wild-type, pH 7.0659065 2D-image
840-PropionateMethanosarcina thermophila-mutant V93G, pH 7.0659065 2D-image
1029-PropionateMethanosarcina thermophila-mutant V93A, pH 7.0659065 2D-image

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.24-adenosine 5'-(gamma-thio)triphosphateMethanosarcina thermophila-pH 7.0659446 2D-image

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
0.28-Methanosarcina thermophila-mutant D148A642196
1.71-Geobacillus stearothermophilus--642172
2.4-Propionibacterium freudenreichii--642181
3.16-Rhodopseudomonas palustris--642183
3.4-Methanosarcina thermophila-mutant E384A642196
4-Methanosarcina thermophila-mutant E384A642191
9-Methanosarcina thermophila-mutant S10A642196
13-Methanosarcina thermophila-mutant N7A642196
19-Methanosarcina thermophila-mutant S12A642196
19.6-Desulfovibrio vulgaris-isoenzyme AK-II642197
24-Desulfovibrio vulgaris--642186
27.7-Clostridium acetobutylicum--642187
28.3-Desulfovibrio vulgaris-isoenzyme AK-I642197
42.12-Ethanoligenens harbinenseQ2N1I6at 30°C673209
102-Methanosarcina thermophila-mutant K14A642196
205.9-Acholeplasma laidlawii--642176
282-Streptococcus pyogenes--642166
324-Methanosarcina thermophila-mutant S11A642196
355-Veillonella parvula--642174, 642178
375500Veillonella parvula--642171
490-Clostridium thermocellum--642190
539-Methanosarcina thermophila-mutant N211A642196
584-Methanosarcina thermophila-wild-type642196
656-Methanosarcina thermophila--642184
948-Veillonella parvula--642173
1087-Clostridium acetobutylicum--642189
1530-Geobacillus stearothermophilus--642180
additional information-Lactobacillus sanfranciscensisQ9F1X7190 U/mg642195
additional information-Methanosarcina thermophila-catalytic mechanism analyzed in wild-type and mutant variants, binding constants for the nucleotide substrates indicate that Arg241 is involved in transition state stabilization and not directly involved in nucleotide recognition or binding, or in the domain closure required for catalysis, binding constants of the nucleotide substrates for Arg91 suggest that this residue has a role in transition state stabilization, evidence for domain motion dependent upon nucleotide ligand binding presented, suggestion that Arg91 is important for closure of domain I onto domain II for catalysis690888
additional information-Thermotoga maritimaQ9WYB1continuous assay, spectrophotometric quantification of phosphate achieved through measurement of the phosphorylysis of 2-amino-6-mercapto-7-methyl-purine riboside (MESG) to ribose-1-phosphate and 2-amino-6-mercapto-7-methyl-purine (MES) by purine nucleoside phosphorylase, sensitivity of the assay is in the range of 2-150 microM691204
additional information-Mycoplasma pneumoniaeP75245transcription initiation and regulation controlled in a carbon source dependent manner, ackA gene encoding acetate kinase is strongly expressed in the presence of glucose, promoter mapping by primer extension, promoter recognition sites studied by promoter deletion analysis, -35 region seems to be of minor importance693619

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
6.4-Escherichia coli--642167
6.57.1Rhodopseudomonas palustris--642183
77.4Methanosarcina thermophila--642184
78Propionibacterium freudenreichii--642181
78.5Desulfovibrio vulgaris--642197
7-Thermotoga maritima--487537
7.210Veillonella parvula--642173
7.28Clostridium thermocellum--642190
7.3-Geobacillus stearothermophilus--642172, 642180
7.5-Escherichia coli--642166
7.5-Propionibacterium freudenreichii--642181
7.5-Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium--642182
7.5-Lactobacillus sanfranciscensisQ9F1X7-642195
7.5-Thermotoga maritimaQ9WYB1assay at691204
8-Clostridium acetobutylicum-30% activity still observed at pH 5.5642187
8.39Veillonella parvula--642174

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
4.511.5Escherichia coli--642167
5.59Methanosarcina thermophila-about 35% of maximum activity at pH 5.5 and 9.0642184
5.59.5Desulfovibrio vulgaris--642197
610Clostridium thermocellum--642190
68.5Thermotoga maritima-about 50% activity in this range487537
69Veillonella parvula--642175
69.5Geobacillus stearothermophilus--642172
6.58.5Thermotoga maritimaQ9WYB1assay can be performed over pH range of691204
78Propionibacterium freudenreichii-most active between642181

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
37-Thermotoga maritimaQ9WYB1assay at691204
50-Clostridium thermocellum--642190
53-Lactobacillus sanfranciscensisQ9F1X7-642195
55-Propionibacterium freudenreichii--642181
60-Moorella thermoacetica--642170
90-Thermotoga maritima--487537

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
080Lactobacillus sanfranciscensisQ9F1X7-642195
2080Clostridium thermocellum--642190
3070Methanosarcina thermophila--642184
40100Thermotoga maritima-activity increases rapidly above 55°C, enzyme does not lose activity at 80°C upon incubation for 180 min487537

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
4.74.8Methanosarcina thermophila--642184
4.9-Rhodopseudomonas palustris--642183
5.93-Ethanoligenens harbinenseQ2N1I6calculated from amino acid sequence673209

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
No entries in this field

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
cytoplasmMethanosarcina thermophila--5737642184Manually annotated by BRENDA team

PDBSCOPCATHORGANISM
1lrg, downloadSCOP (1lrg)CATH (1lrg)Escherichia coli (strain K12), Escherichia coli O157:H7, Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
1g99, downloadSCOP (1g99)CATH (1g99)Methanosarcina thermophila
1tuu, downloadSCOP (1tuu)CATH (1tuu)Methanosarcina thermophila
1tuy, downloadSCOP (1tuy)CATH (1tuy)Methanosarcina thermophila
3p4i, downloadSCOP (3p4i)CATH (3p4i)Mycobacterium avium (strain 104)
4dq8, downloadSCOP (4dq8)CATH (4dq8)Mycobacterium marinum (strain ATCC BAA-535 / M)
1lp2, downloadSCOP (1lp2)CATH (1lp2)Pasteurella multocida (strain Pm70)
3sk3, downloadSCOP (3sk3)CATH (3sk3)Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
3slc, downloadSCOP (3slc)CATH (3slc)Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
2iir, downloadSCOP (2iir)CATH (2iir)Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
11900-Acholeplasma laidlawii-gel filtration, Sephadex G200642176
43220-Ethanoligenens harbinenseQ2N1I6calculated from amino acid sequence673209
45000-Lactobacillus sanfranciscensisQ9F1X7SDS-PAGE642195
46500-Rhodopseudomonas palustris-gel filtration642183
47000-Rhodopseudomonas palustris-gel filtration642183
48000-Rhodopseudomonas palustris-gel filtration642183
57000-Lactobacillus sanfranciscensisQ9F1X7gel filtration642195
60000-Moorella thermoacetica-gel filtration642170
64000-Acholeplasma laidlawii-sucrose density gradient ultracentrifugation, 0.5 M NaCl642176
66000-Veillonella parvula-gel filtration642173
68000-Salmonella enterica subsp. enterica serovar Typhimurium-gel filtration642182
70000-Escherichia coli-gel filtration642182
71000-Acholeplasma laidlawii-gel filtration, Sephadex G200, 0.5 M NaCl642176
76400-Desulfovibrio vulgaris-isoenzyme AK-I, gel filtration642197
78000-Clostridium acetobutylicum-gel filtration642187
84000-Clostridium thermocellum-gel filtration642190
8700094000Clostridium acetobutylicum-gel filtration, native gel electrophoresis642189
87000-Veillonella parvula-gel filtration642171
88000-Veillonella parvula-gel filtration642174, 642178
89000-Clostridium acetobutylicum-native PAGE642189
90000-Thermotoga maritima-gel filtration487537
94000-Methanosarcina thermophila-gel filtration642184
110000-Spirochaeta sp.-sucrose density gradient centrifugation, isoenzyme I and II642179
125000-Spirochaeta sp.-gel filtration, isoenzyme I and II642179
130000-Acholeplasma laidlawii-sucrose density gradient ultracentrifugation642176
160000170000Geobacillus stearothermophilus-gel filtration642180
160000-Geobacillus stearothermophilus-gel filtration642172
170000-Geobacillus stearothermophilus-sedimentation analysis642172
240000-Propionibacterium freudenreichii-gel filtration642181

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
dimerThermotoga maritima-2 * 44000, alpha2 homodimer, SDS-PAGE487537
dimerVeillonella parvula-2 * 44000, SDS-PAGE642171
dimerVeillonella parvula-2 * 43000, SDS-PAGE642173
dimerVeillonella parvula-2 * 42000, homodimer, SDS-PAGE642174, 642178
dimerAcholeplasma laidlawii-2 * 51000, SDS-PAGE642176
dimerEscherichia coli-2 * 40000, SDS-PAGE642182
dimerSalmonella enterica subsp. enterica serovar Typhimurium-2 * 41200, SDS-PAGE642182
dimerMethanosarcina thermophila-2 * 53000, alpha2 homodimer642184
dimerClostridium acetobutylicum-2 * 42000, SDS-PAGE642187
dimerClostridium acetobutylicum-2 * 43000, SDS-PAGE642189
dimerClostridium thermocellum-2 * 46000, homodimer, SDS-PAGE642190
dimerMethanosarcina thermophila--642191
dimerDesulfovibrio vulgaris-1 * 49300 + 1 * 47800, isoenzyme AK-II, alphaSalphaL heterodimer, SDS-PAGE; 2 * 47800, isoenzyme AK-I, alphaS2 homodimer, SDS-PAGE642197
hexamerPropionibacterium freudenreichii-6 * 41000, SDS-PAGE642181
monomerRhodopseudomonas palustris-1 * 45000, SDS-PAGE642183
tetramerGeobacillus stearothermophilus-4 * 43000, SDS-PAGE642172, 642180

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-Geobacillus stearothermophilus-642172, 642180
-Methanosarcina thermophila-659065
in presence of substrate and transition states analoguesMethanosarcina thermophila-659446
space group C2, unit cell dimensions a = 181 A, b = 67 A, c = 83 A, beta 103 degreesMethanosarcina thermophila-642188

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
510Clostridium thermocellum-quite stable between pH 7.0 to 9.0, only 15% loss in activity at pH 10.0, inactivated rapidly outside this range642190
5.58.5Propionibacterium freudenreichii-stable in this range642181
611Geobacillus stearothermophilus-rather stable at alkaline pH but rapidly inactivated at acidic pH lower than 6.0, about 40% loss of activity at pH 11.0642172
6-Escherichia coli-irreversible inactivated below642167
6.57.8Escherichia coli-enzyme undergoes irreversible inactivation outside this range642166
78Geobacillus stearothermophilus-stable, rapid inactivation at pH values lower than 6.0, rather stable at alkaline pH642180
7.48Veillonella parvula-stable in buffers of pH 7.4 and 8.0, unstable under preincubation at pH 5.5642173

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
3560Propionibacterium freudenreichii-84% remaining activity at 35°C, 49% at 45°C, almost zero at 60°C642181
5070Veillonella parvula-with treatment at 50°C for 20 min 73% enzyme activity retained, treatment by heat at 60°C for 5 min results in 60% loss of activity, 70°C for 5 min results in complete loss of activity642173
55100Thermotoga maritima-almost complete loss about 90% is observed after incubation at 100°C for about 60 min, enzyme does not lose activity upon incubation for 3 h at 100°C in presence of 1 M (NH4)2SO4487537
6080Clostridium thermocellum-retains full activity, stable up to 60°C, loses 55% of its activity at 70°C, completely inactivated after 2 h at 80°C642190
6090Lactobacillus sanfranciscensisQ9F1X7stable for 30 min at 60°C, loses activity after 30 min at 70°C, 10 min at 80°C and 5 min at 90°C642195
60-Rhodopseudomonas palustris-10 min incubation causes a loss in activity of 50%642183
65-Geobacillus stearothermophilus-quite stable up to642172
65-Geobacillus stearothermophilus-quite heat stable642180
70-Methanosarcina thermophila-stable to heating for 15 min, but rapidly inactivated at higher temperatures642184

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
almost no decrease in activity after several freezing and thawing cyclesAcholeplasma laidlawii-642176
ATP and acetate protects against inactivationAcinetobacter calcoaceticus-642185
ATP, ADP and AMP protects against inactivation by N-ethylmaleimideEscherichia coli-642177
destroyed by repeated freezing and thawingEscherichia coli-642182
stabilized during storage by L-cysteineEscherichia coli-642166
ATP or ADP protects the enzyme from inactivationGeobacillus stearothermophilus-642172
all 4 substrates of the forward and reverse reaction protect the enzyme from inactivation by diethyldicarbonateMethanosarcina thermophila-642184
unaffected by repeated freezing and thawingMethanosarcina thermophila-642184
destroyed by repeated freezing and thawingSalmonella enterica subsp. enterica serovar Typhimurium-642182
stabilized against heat inactivation by salts, most effective (NH4)2SO4Thermotoga maritima-487537

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
sensitive to light oxidationGeobacillus stearothermophilus-642172
stable to O2Methanosarcina thermophila-642184
not sensitive to oxygenThermotoga maritima-487537

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-20°C, almost no decrease in activity within 1 year of storageAcholeplasma laidlawii-642176
-20°C, can be stored in 50 mM Tris-HCl, pH 7.4, 0.3 M NaCl and 2 mM dithiothreitol for several months without loss of activityDesulfovibrio vulgaris-642197
-20°C, relatively stable to storage when frozen, remains active for at least 1 yearEscherichia coli-642182
0°C, even mild chilling overnight disrupts the protein conformationEscherichia coli-642167
-196°C, stable for at least 4 months in liquid nitrogenMethanosarcina thermophila-642184
-24°C, can be stored for several months with only a negligible loss in activityRhodopseudomonas palustris-642183
0-4°C, can be stored for some days with only a negligible loss in activityRhodopseudomonas palustris-642183
-20°C, can be stored in 20 mM Tris-HCl, pH 8.0, 2.0 mM MgCl2, 0.32 M NaCl, supplemented with 10% glycerol, without significant loss of activity for several weeksThermotoga maritima-487537
-18°C, buffer containing 0.25 M sucrose, 40% of initial enzyme activity is lost within 2 monthsVeillonella parvula-642173
-45°C, buffer containing 0.25 M sucrose, seems to be more stable than at -18°CVeillonella parvula-642173
4°C, 100 mM KCl, 50 mM disodium succinate, 20 mM TEA, pH 8.3, stable for at least 4 weeksVeillonella parvula-642178

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-Acholeplasma laidlawii-642176
-Acinetobacter calcoaceticus-642185
-Clostridium acetobutylicum-642187, 642189
-Clostridium thermocellum-642190
-Desulfovibrio vulgaris-642186
2 isoenzymes purifiedDesulfovibrio vulgaris-642197
-Escherichia coli-642166, 642167
partialEscherichia coli-642182
using imidazole elution [20 mM Tris-HCl, pH 7.9, 0.5 M NaCl, 10% glycerol, 8 M urea, 1 mM PMSF (pH 7.9)] buffers containing final concentrations of imidazole (100 mM, 200 mM, and 1 M imidazole)Ethanoligenens harbinenseQ2N1I6673209
-Geobacillus stearothermophilus-642172, 642180
recombinant enzymeLactobacillus sanfranciscensisQ9F1X7642195
-Methanosarcina thermophila-642184, 642193, 659856
judged by SDS-PAGEMethanosarcina thermophila-690888
purified wild-type and recombinant enzymes, R175L and R340A are unstable and cannot be purifiedMethanosarcina thermophila-642194
-Moorella thermoacetica-642170
gel filtrationMycoplasma pneumoniaeP75245693619
-Propionibacterium freudenreichii-642181
-Rhodopseudomonas palustris-642183
-Salmonella enterica subsp. enterica serovar Typhimurium-642182
-Streptococcus pyogenes-642166
-Thermotoga maritima-487537
gel filtrationThermotoga maritimaQ9WYB1691204
-Veillonella parvula-642173, 642174, 642178

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
DNA fragment encoding 246 bp of the acetate kinase gene of strain DSM 792 cloned by a PCR-based approachClostridium acetobutylicum-642189
expressed in Escherichia coli BL21(DE3) cellsEthanoligenens harbinenseQ2N1I6673209
ack gene identified by PCR, heterologously expressed in Escherichia coli JM 109Lactobacillus sanfranciscensisQ9F1X7642195
cloned and hyper-expressed in Escherichia coliMethanosarcina thermophila-642196
overexpressed in Escherichia coli BL21(DE3)Methanosarcina thermophila-690888
wild-type and mutant acetate kinase genes subcloned in to the T7-based expression vector pET15bMethanosarcina thermophila-642191, 642194
wild-type and variant acetate kinases are overproduced in Escherichia coli BL21(DE3)Methanosarcina thermophila-642193
expressed in Escherichia coli, reporter system used that allows cloning of promoter fragments in front of a promoterless lacZ gene and insertion in Mycoplasma pneumoniaeMycoplasma pneumoniaeP75245693619

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
D148AMethanosarcina thermophila-site-directed mutagenesis642196
D148EMethanosarcina thermophila-site-directed mutagenesis642196
D148NMethanosarcina thermophila-site-directed mutagenesis642196
E384AMethanosarcina thermophila-site-directed mutagenesis642191, 642196
E384DMethanosarcina thermophila-site-directed mutagenesis642191
E384QMethanosarcina thermophila-site-directed mutagenesis642191
E97DMethanosarcina thermophila-reduction of both Km and kcat-value659856
F179AMethanosarcina thermophila-reduced catalytic efficiency with all substrates tested659065
H123AMethanosarcina thermophila-site-directed mutagenesis642193
H152AMethanosarcina thermophila-site-directed mutagenesis642193
H180AMethanosarcina thermophila-site-directed mutagenesis642193
H180RMethanosarcina thermophila-site-directed mutagenesis642193
H184AMethanosarcina thermophila-site-directed mutagenesis642193
H208AMethanosarcina thermophila-site-directed mutagenesis642193
H60AMethanosarcina thermophila-site-directed mutagenesis642193
H90AMethanosarcina thermophila-site-directed mutagenesis642193
H94AMethanosarcina thermophila-site-directed mutagenesis642193
K14AMethanosarcina thermophila-site-directed mutagenesis642196
K14RMethanosarcina thermophila-site-directed mutagenesis642196
L122AMethanosarcina thermophila-reduced catalytic efficiency with all substrates tested659065
N211AMethanosarcina thermophila-site-directed mutagenesis642196
N7AMethanosarcina thermophila-site-directed mutagenesis642196
P232AMethanosarcina thermophila-reduced catalytic efficiency with all substrates tested659065
Q43WMethanosarcina thermophila-site-directed mutagenesis, single mutant690888
R175KMethanosarcina thermophila-site-directed mutagenesis642194
R241AMethanosarcina thermophila-severe decrease in kinetic parameters659446
R241A/Q43WMethanosarcina thermophila-site-directed mutagenesis, double mutant690888
R241K/Q43WMethanosarcina thermophila-site-directed mutagenesis, double mutant690888
R241LMethanosarcina thermophila-severe decrease in kinetic parameters659446
R285AMethanosarcina thermophila-site-directed mutagenesis642194
R285KMethanosarcina thermophila-site-directed mutagenesis642194
R285LMethanosarcina thermophila-site-directed mutagenesis642194
R340KMethanosarcina thermophila-site-directed mutagenesis642194
R340LMethanosarcina thermophila-site-directed mutagenesis642194
R91AMethanosarcina thermophila-severe decrease in kinetic parameters659446
R91A/Q43WMethanosarcina thermophila-site-directed mutagenesis, double mutant690888
R91KMethanosarcina thermophila-site-directed mutagenesis642194
R91KMethanosarcina thermophila-severe decrease in kinetic parameters659446
R91K/Q43WMethanosarcina thermophila-site-directed mutagenesis, double mutant690888
R91LMethanosarcina thermophila-severe decrease in kinetic parameters659446
S10AMethanosarcina thermophila-site-directed mutagenesis642196
S11AMethanosarcina thermophila-site-directed mutagenesis642196
S12AMethanosarcina thermophila-site-directed mutagenesis642196
V93AMethanosarcina thermophila-improved catalytic efficiency with propionate and butyrate659065
V93GMethanosarcina thermophila-improved catalytic efficiency with propionate and butyrate659065
additional informationEscherichia coli-mutant with no enzymic activity, fails to grow in xylose minimal medium under anaerobic conditions but grows anaerobically with arabinose659056

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
using 1 mol/l urea, 50 mM Tris-HCl, pH 8.0, 10 mM reduced glutathione, 2 mmol GSSG, 1 mM FeCl2, 2 mM dithiothreitol, and 10% glycerol, the renaturation yield is 14.36%Ethanoligenens harbinenseQ2N1I6673209

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
industryClostridium thermocellum-enzyme involved in the wasteful production of acetate during conversion of cellulose to ethanol642190
synthesisMethanosarcina thermophila-protocol for overproduction of enzyme659856

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISMLINK TO PUBMEDSOURCE
487537Bock, A.K.; Glasemacher, J.; Schmidt, R.; Schonheit, P.Purification and characterization of two extremely thermostable enzymes, phosphate acetyltransferase and acetate kinase, from the hyperthermophilic eubacterium Thermotoga maritimaJ. Bacteriol.1811861-18671999Thermotoga maritima PubMed
642166Rose, I.A.Acetate kinaseThe Enzymes, 2nd. Ed. (Boyer, P.D., Lardy, H., Myrbaeck, K., eds.)6115-1181962Escherichia coli, Lactobacillus delbrueckii, Streptococcus pyogenes-
642167Anthony, R.S.; Spector, L.B.Exchange reactions catalyzed by acetate kinaseJ. Biol. Chem.2466129-61351971Escherichia coli PubMed
642168Janson, C.A.; Cleland, W.W.The inhibition of acetate, pyruvate, and 3-phosphogylcerate kinases by chromium adenosine triphosphateJ. Biol. Chem.2492567-25711974Escherichia coli PubMed
642169Janson, C.A.; Cleland, W.W.The specificity of chromium nucleotides as inhibitors of selected kinasesJ. Biol. Chem.2492572-25741974Escherichia coli PubMed
642170Schaupp, A.; Ljungdahl, L.G.Purification and properties of acetate kinase from Clostridium thermoaceticumArch. Microbiol.100121-1291974Moorella thermoacetica PubMed
642171Bowman, C.M.; Valdez, R.O.; Nishimura, J.S.Acetate kinase from Veillonella alcalescens. Regulation of enzyme activity by succinate and substratesJ. Biol. Chem.2513117-31211976Veillonella parvula PubMed
642172Nakajima, H.; Suzuki, K.; Imahori, K.Purification and properties of acetate kinase from Bacillus stearothermophilusJ. Biochem.84193-2031978Geobacillus stearothermophilus PubMed
642173Yoshimura, F.Purification and characterization of acetate kinase from Veillonella alcalescens ATCC 17748Arch. Biochem. Biophys.189424-4321978Veillonella parvula PubMed
642174Griffith, M.J.; Nishimura, J.S.Acetate kinase from Veillonella alcalescens. Purification and physical propertiesJ. Biol. Chem.254442-4461979Veillonella parvula PubMed
642175Griffith, M.J.; Nishimura, J.S.Acetate kinase from Veillonella alcalescens. Regulation by succinate and substratesJ. Biol. Chem.2546698-67021979Veillonella parvula PubMed
642176Kahane, I.; Muhlrad, A.Purification and properties of acetate kinase from Acholeplasma laidlawiiJ. Bacteriol.137764-7721979Acholeplasma laidlawii PubMed
642177Wong, S.S.; Wong, L.J.C.Inactivation of Escherichia coli acetate kinase by N-ethylmaleimide. Protection by substrates and productsBiochim. Biophys. Acta615121-1311980Escherichia coli PubMed
642178Nishimura, J.S.; Griffith, M.J.Acetate kinase from Veilonella alcalescensMethods Enzymol.71311-3161981Veillonella parvula-
642179Harwood, C.S.; Canale-Parola, E.Properties of acetate kinase isozymes and a branched-chain fatty acid kinase from a spirocheteJ. Bacteriol.152246-2541982Spirochaeta sp. PubMed
642180Suzuki, K.; Nakajima, H.; Imahori, K.Acetate kinase from Bacillus stearothermophilusMethods Enzymol.90179-1851982Geobacillus stearothermophilus-
642181Ichikawa, Y.; Mori, N.; Miki, S.; Hosoi, N.; Kitamoto, Y.Purification and properties of acetate kinase from propionibacterium freudenreichiiJ. Ferment. Technol.63143-1491985Propionibacterium freudenreichii-
642182Fox, D.K.; Roseman, S.Isolation and characterization of homogeneous acetate kinase from Salmonella typhimurium and Escherichia coliJ. Biol. Chem.26113487-134971986Escherichia coli, Salmonella enterica subsp. enterica serovar Typhimurium PubMed
642183Vigenschow, H.; Schwarm, H.M.; Knobloch, K.Purification and properties of an acetate kinase from Rhodopseudomonas palustrisBiol. Chem. Hoppe-Seyler367951-9561986Rhodopseudomonas palustris PubMed
642184Aceti, D.J.; Ferry, J.G.Purification and characterization of acetate kinase from acetate-grown Methanosarcina thermophilaJ. Biol. Chem.24915444-154481988Methanosarcina thermophila-
642185Kim, Y.S.; Park, C.Inactivation of Acinetobacter calcoaceticus acetate kinase by diethylpyrocarbonateBiochim. Biophys. Acta956103-1091988Acinetobacter calcoaceticus PubMed
642186Mannens, G.; Slegers, G.; Claeys, A.Purification and immobilization of acetate kinase from Desulfovibrio vulgarisBiotechnol. Lett.10563-5681988Desulfovibrio vulgaris-
642187Diez-Gonzalez, F.; Russell, J.B.; Hunter, J.B.The acetate kinase of Clostridium acetobutylicum strain P262Arch. Microbiol.166418-4201996Clostridium acetobutylicum PubMed
642188Buss, K.A.; Ingram-Smith, C.; Ferry, J.G.; Sanders, D.A.; Hasson, M.S.Crystallization of acetate kinase from Methanosarcina thermophila and prediction of its foldProtein Sci.62659-26621997Methanosarcina thermophila PubMed
642189Winzer, K.; Lorenz, K.; Duerre, P.Acetate kinase from Clostridium acetobutylicum: a highly specific enzyme that is actively transcribed during acidogenesis and solventogenesisMicrobiology1433279-32861997Clostridium acetobutylicum-
642190Lin, W.R.; Peng, Y.; Lew, S.; Lee, C.C.; Hsu, J.J.; Hamel, J.F.; Demain, A.L.Purification and characterization of acetate kinase from Clostridium thermocellumTetrahedron5415915-159251998Clostridium thermocellum-
642191Singh-Wissmann, K.; Ingram-Smith, C.; Miles, R.D.; Ferry, J.G.Identification of essential glutamates in the acetate kinase from Methanosarcina thermophilaJ. Bacteriol.1801129-11341998Methanosarcina thermophila PubMed
642193Ingram-Smith, C.; Barber, R.D.; Ferry, J.G.The role of histidines in the acetate kinase from Methanosarcina thermophilaJ. Biol. Chem.27533765-337702000Methanosarcina thermophila PubMed
642194Singh-Wissmann, K.; Miles, R.D.; Ingram-Smith, C.; Ferry, J.G.Identification of essential arginines in the acetate kinase from Methanosarcina thermophilaBiochemistry393671-36772000Methanosarcina thermophila PubMed
642195Knorr, R.; Ehrmann, M.A.; Vogel, R.F.Cloning, expression, and characterization of acetate kinase from Lactobacillus sanfranciscensisMicrobiol. Res.156267-2772001Lactobacillus sanfranciscensis PubMed
642196Miles, R.D.; Iyer, P.P.; Ferry, J.G.Site-directed mutational analysis of active site residues in the acetate kinase from Methanosarcina thermophilaJ. Biol. Chem.27645059-450642001Methanosarcina thermophila PubMed
642197Yu, L.; Ishida, T.; Ozawa, K.; Akutsu, H.; Horiike, K.Purification and characterization of homo- and hetero-dimeric acetate kinases from the sulfate-reducing bacterium Desulfovibrio vulgarisJ. Biochem.129411-4212001Desulfovibrio vulgaris PubMed
642198Ito, K.; Nakagawa, K.; Murakami, S.; Arakawa, H.; Maeda, M.Highly sensitive simultaneous bioluminescent measurement of acetate kinase and pyruvate phosphate dikinase activities using a firefly luciferase-luciferin reaction and its application to a tandem bioluminescent enzyme immunoassayAnal. Sci.19105-1092003Geobacillus stearothermophilus PubMed
659056Hasona, A.; Kim, Y.; Healy, F.G.; Ingram, L.O.; Shanmugam, K.T.Pyruvate formate lyase and acetate kinase are essential for anaerobic growth of Escherichia coli on xyloseJ. Bacteriol.1867593-76002004Escherichia coli PubMed
659065Ingram-Smith, C.; Gorrell, A.; Lawrence, S.H.; Iyer, P.; Smith, K.; Ferry, J.G.Characterization of the acetate binding pocket in the Methanosarcina thermophila acetate kinaseJ. Bacteriol.1872386-23942005Methanosarcina thermophila PubMed
659446Gorrell, A.; Lawrence, S.H.; Ferry, J.G.Structural and kinetic analyses of arginine residues in the active site of the acetate kinase from Methanosarcina thermophilaJ. Biol. Chem.28010731-107422005Methanosarcina thermophila PubMed
659856Iyer, P.; Ferry, J.G.Acetate kinase from Methanosarcina thermophila, a key enzyme for methanogenesisMethods Biotechnol.17239-2462005Methanosarcina thermophila-
672708Suwannakham, S.; Huang, Y.; Yang, S.T.Construction and characterization of ack knock-out mutants of Propionibacterium acidipropionici for enhanced propionic acid fermentationBiotechnol. Bioeng.94383-3952006Propionibacterium acidipropionici PubMed
673209Ren, N.Q.; Lin, H.L.; Zhang, K.; Zheng, G.X.; Duan, Z.J.; Lin, M.Cloning, expression, and characterization of an acetate kinase from a high rate of biohydrogen bacterial strain Ethanoligenens sp. hit B49Curr. Microbiol.55167-1722007Ethanoligenens harbinense PubMed
673750Baldock, M.I.; Denger, K.; Smits, T.H.; Cook, A.M.Roseovarius sp. strain 217: aerobic taurine dissimilation via acetate kinase and acetate-CoA ligaseFEMS Microbiol. Lett.271202-2062007Roseovarius sp. PubMed
677237Ingram-Smith, C.; Martin, S.R.; Smith, K.S.Acetate kinase: not just a bacterial enzymeTrends Microbiol.14249-2532006Ajellomyces capsulatus, Aspergillus fumigatus, Bacillus subtilis, Borrelia burgdorferi, Chaetomium globosum, Chlamydomonas reinhardtii, Coccidioides immitis, Coprinopsis cinerea, Cryptococcus neoformans, Cupriavidus necator, Emericella nidulans, Entamoeba histolytica, Enterococcus faecalis, Gibberella zeae, Helicobacter pylori, Hypocrea jecorina, Lactobacillus acidophilus, Lactococcus lactis, Listeria monocytogenes, Magnaporthe grisea, Mesoplasma florum, Methanosarcina acetivorans, Methanosarcina mazei, Methanosarcina thermophila, Mycoplasma pneumoniae, Neurospora crassa, Oceanobacillus iheyensis, Phaeosphaeria nodorum, Phanerochaete chrysosporium, Phytophthora ramorum, Phytophthora sojae, Salmonella enterica, Sclerotinia sclerotiorum, Staphylococcus aureus, Streptococcus pneumoniae, Uncinocarpus reesii, Ustilago maydis PubMed
690888Gorrell, A.; Ferry, J.G.Investigation of the Methanosarcina thermophila acetate kinase mechanism by fluorescence quenchingBiochemistry4614170-141762007Methanosarcina thermophila PubMed
691204Mukhopadhyay, S.; Hasson, M.S.; Sanders, D.A.A continuous assay of acetate kinase activity: measurement of inorganic phosphate release generated by hydroxylaminolysis of acetyl phosphateBioorg. Chem.3665-692008Thermotoga maritima PubMed
693619Halbedel, S.; Eilers, H.; Jonas, B.; Busse, J.; Hecker, M.; Engelmann, S.; Stuelke, J.Transcription in Mycoplasma pneumoniae: analysis of the promoters of the ackA and ldh genesJ. Mol. Biol.371596-6072007Mycoplasma pneumoniae PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 2.7.2.1)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)