Information on EC 2.7.1.86

Information on EC 2.7.1.86 - NADH kinase:

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EC NUMBER	COMMENTARY
2.7.1.86	-

RECOMMENDED NAME	GeneOntology No.
NADH kinase	GO:0042736

REACTION	REACTION DIAGRAM	COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
ATP + NADH = ADP + NADPH		-	-	-	-

REACTION TYPE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
phospho group transfer	-	-	-	-

PATHWAY	KEGG Link	MetaCyc Link
NAD/NADH phosphorylation and dephosphorylation	-	PWY-5083

SYSTEMATIC NAME	IUBMB Comments
ATP:NADH 2'-phosphotransferase	CTP, ITP, UTP and GTP can also act as phosphate donors (in decreasing order of activity). The enzyme is specific for NADH. Activated by acetate.

SYNONYMS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
ATP-NADH kinase	Saccharomyces cerevisiae	-	phosphorylates both NAD+ and NADH	661696
ATP-NADH kinase	Micrococcus flavus, Micrococcus flavus AKU 0502, Micrococcus flavus ICR 1820, Mycobacterium tuberculosis	-	-	662376
ATP-NADH kinase	Fusarium oxysporum	-	-	706568
ATP:NADH 2'-phosphotransferase	-	-	-	-
ATP:NADH 2'-phosphotransferase	Saccharomyces cerevisiae	P32622	-	695829
ATP:NADH 2'-phosphotransferase	Fusarium oxysporum	-	-	706568
ATP:NADH2 2'-phosphotransferase	-	-	-	-
diphosphopyridine nucleotide (reduced) kinase	Arabidopsis thaliana	-	-	657853
DPNH kinase	-	-	-	-
kinase, reduced nicotinamide adenine dinucleotide (phosphorylating)	-	-	-	-
Mfnk	Micrococcus flavus AKU 0502, Micrococcus flavus, Micrococcus flavus ICR 1820	-	exhibits polyphosphate- and ATP-dependent NADH kinase activities	662376
NAD(H) kinase	Arabidopsis thaliana	-	-	657853
NAD(H)K	Arabidopsis thaliana	Q56YN3	-	676008
NADH kinase	-	-	-	-
NADH kinase	Emericella nidulans	-	-	693998
NADH kinase POS5, mitochondrial	Saccharomyces cerevisiae	-	-	674616
NADH2 kinase	-	-	-	-
NADHK	Arabidopsis thaliana	-	-	657853
NADK-1	Arabidopsis thaliana	Q56YN3	-	676008
NADK1	Arabidopsis thaliana	-	isoform	691390
NADK3	Arabidopsis thaliana	-	-	657853, 676516, 706395
nicotinamide adenine dinucleotide (reduced) kinase	Arabidopsis thaliana	-	-	657853
Pos5	Saccharomyces cerevisiae	-	;	674616
Pos5	Saccharomyces cerevisiae	P32622	-	695829
Pos5p	Saccharomyces cerevisiae	C7GJD6	-	691390
Ppnk	Mycobacterium tuberculosis	-	exhibits polyphosphate- and ATP-dependent NADH kinase activities	662376
reduced diphosphopyridine nucleotide kinase	-	-	-	-
reduced nicotinamide adenine dinucleotide kinase (phosphorylating)	-	-	-	-
Utr1	Saccharomyces cerevisiae	Q06892	-	674616, 695829
YEF1	Saccharomyces cerevisiae	-	-	661696, 674616, 695829
Yef1p	Saccharomyces cerevisiae	-	-	661696
YEL041W	Saccharomyces cerevisiae	-	-	674616

CAS REGISTRY NUMBER	COMMENTARY
62213-39-2	-

ORGANISM	COMMENTARY	LITERATURE	SEQUENCE CODE	SEQUENCE DB	SOURCE
Arabidopsis thaliana	-	691390, 706395	-	-
Arabidopsis thaliana	; first discovery in plants	657853	-	-
Arabidopsis thaliana	ecotype Col	676516	-	-
Arabidopsis thaliana	ecotype Ws-4	676008	Q56YN3	SwissProt
Emericella nidulans	strain CBS 513.88 /FGSC A1513	693998	-	-
Emericella nidulans CBS 513.88 /FGSC A1513	strain CBS 513.88 /FGSC A1513	693998	-	-
Fusarium oxysporum	strain F3	706568	-	-
Fusarium oxysporum F3	strain F3	706568	-	-
Micrococcus flavus	-	662376	-	-
Mycobacterium tuberculosis	strain H37Rv	662376	-	-
Pichia membranifaciens	-	642063	-	-
Saccharomyces cerevisiae	-	642062, 649040, 649041, 661696, 674616, 695829	-	-
Saccharomyces cerevisiae	-	674616, 695829	Q06892	SwissProt
Saccharomyces cerevisiae	-	691390	C7GJD6	UniProt
Saccharomyces cerevisiae	-	695829	P32622	SwissProt
Saccharomyces cerevisiae	strain X2181-1A	642064, 642065, 642066	-	-
Saccharomyces cerevisiae	strain YPH925	705593	-	-
Saccharomyces cerevisiae X2181-1A	strain X2181-1A	642064, 642065, 642066	-	-
Saccharomyces cerevisiae YPH925	strain YPH925	705593	-	-

GENERAL INFORMATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
malfunction	Saccharomyces cerevisiae	-	yeast cells lacking the mitochondrial NADH kinase encoded by POS5 display increased sensitivity to hydrogen peroxide, a slow-growth phenotype, reduced mitochondrial function and increased levels of mitochondrial protein oxidation and mitochondrial DNA mutations	705593

SUBSTRATE	PRODUCT	REACTION DIAGRAM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY/ Substrate	LITERATURE/ Substrate	COMMENTARY/ Product	LITERATURE/ Product	Reversibility r=reversible ir=irreversible ?=not specified
ATP + NAD+	ADP + NADP+		Saccharomyces cerevisiae	-	-	661696	-	-	?
ATP + NAD+	ADP + NADP+		Saccharomyces cerevisiae	Q06892	-	674616	-	-	?
ATP + NAD+	ADP + NADP+		Emericella nidulans	-	-	693998	-	-	?
ATP + NAD+	ADP + NADP+		Arabidopsis thaliana	Q56YN3	-	676008	-	-	?
ATP + NAD+	ADP + NADP+		Saccharomyces cerevisiae	C7GJD6	-	691390	-	-	?
ATP + NAD+	ADP + NADP+		Saccharomyces cerevisiae	-	2fold preference for NADH over NAD+	649040	-	649040	?
ATP + NAD+	ADP + NADP+		Saccharomyces cerevisiae	-	activity with NAD+ is 50fold lower than with NADH	649041	-	-	?
ATP + NAD+	ADP + NADP+		Arabidopsis thaliana	-	NADH is preferred 100fold over NAD+	657853	-	-	?
ATP + NAD+	ADP + NADP+		Emericella nidulans	-	phenotypic effects during overexpression analyzed by simulation of major metabolic fluxes	693998	-	-	?
ATP + NAD+	ADP + NADH		Saccharomyces cerevisiae	P32622	-	695829	-	-	?
ATP + NADH	ADP + NADPH		Saccharomyces cerevisiae	-	-	642064	-	642064	?
ATP + NADH	ADP + NADPH		Saccharomyces cerevisiae	-	-	642065	-	642065	?
ATP + NADH	ADP + NADPH		Saccharomyces cerevisiae	-	-	649041, 661696	-	-	?
ATP + NADH	ADP + NADPH		Saccharomyces cerevisiae	Q06892	-	674616	-	-	?
ATP + NADH	ADP + NADPH		Saccharomyces cerevisiae	-	-	705593	-	-	?
ATP + NADH	ADP + NADPH		Arabidopsis thaliana	-	-	676516	-	-	?
ATP + NADH	ADP + NADPH		Mycobacterium tuberculosis, Micrococcus flavus	-	-	662376	-	-	?
ATP + NADH	ADP + NADPH		Saccharomyces cerevisiae	P32622	-	695829	-	-	?
ATP + NADH	ADP + NADPH		Saccharomyces cerevisiae	-	specific for NADH	642062	-	642062	?
ATP + NADH	ADP + NADPH		Saccharomyces cerevisiae	-	specific for NADH	642066	-	642066	?
ATP + NADH	ADP + NADPH		Pichia membranifaciens	-	specific for NADH	642063	-	642063	?
ATP + NADH	ADP + NADPH		Saccharomyces cerevisiae	-	NAD+ completely ineffective	642066	-	642066	?
ATP + NADH	ADP + NADPH		Saccharomyces cerevisiae	-	2fold preference for NADH over NAD+	649040	-	649040	?
ATP + NADH	ADP + NADPH		Saccharomyces cerevisiae	-	may be involved in maintaining the level of triphosphopyridine nucleotide in the mitochondria	642062	-	642062	?
ATP + NADH	ADP + NADPH		Saccharomyces cerevisiae	-	the enzyme has primarily NADH kinase activity in mitochondria, mitochondrial NADPH is largely provided by the POS5 NADH kinase	649041	-	649041	?
ATP + NADH	ADP + NADPH		Arabidopsis thaliana	-	preferentially phosphorylates reduced NAD+ but can use oxidized NAD+. ATP is preferred phophoryl donor but UTP, GTP, and CTP may substitute with lower activity, NADH is preferred 100fold over NAD+	657853	-	-	?
ATP + NADH	ADP + NADPH		Saccharomyces cerevisiae	Q06892	phosphorylates NADH with 2% of the specific activity of its NAD kinase activity, possesses 33% activity with NADH and ATP compared to the activity obtained with NAD and ATP	674616	-	-	?
ATP + NADH	ADP + NADPH		Arabidopsis thaliana	Q56YN3	shows a 2fold preference for NADH over NAD+	676008	-	-	?
ATP + NADH	ADP + NADPH		Saccharomyces cerevisiae	Q06892	Pos5 is responsible for all mitochondrial NAD/NADH kinase. Transitory exposure of Pos5 to the cytoplasm is required for the viability of utr1 mutants	674616	-	-	?
ATP + NADH	ADP + NADPH		Arabidopsis thaliana	-	isoform NADK1 shows 169% activity compared to NAD+	691390	-	-	?
ATP + NADH	ADP + NADPH		Saccharomyces cerevisiae	C7GJD6	isoform Pos5p shows 167% activity compared to NAD+	691390	-	-	?
ATP + NADH	ADP + NADPH		Arabidopsis thaliana	-	prefers NADH as a substrate, but can also use NAD+ with a much lower efficiency	706395	-	-	?
ATP + NADH	ADP + NADPH		Fusarium oxysporum	-	uses NADH as the preferred diphosphonicotinamide nucleotide donor	706568	-	-	?
ATP + NADH	ADP + NADPH		Pichia membranifaciens H 112	-	specific for NADH	642063	-	642063	?
CTP + NADH	CDP + NADPH		Saccharomyces cerevisiae	-	67% of activity with ATP	642062	-	-	?
CTP + NADH	CDP + NADPH		Arabidopsis thaliana	-	ATP is preferred substrate	657853	-	-	?
GTP + NADH	GDP + NADPH		Saccharomyces cerevisiae	-	45% of activity with ATP	642062	-	-	?
GTP + NADH	GDP + NADPH		Arabidopsis thaliana	-	ATP is preferred substrate	657853	-	-	?
tetrapolyphosphate + NADH	tripolyphosphate + NADPH		Mycobacterium tuberculosis, Micrococcus flavus	-	-	662376	-	-	?
UTP + NADH	UDP + NADPH		Saccharomyces cerevisiae	-	50% of activity with ATP	642062	-	-	?
UTP + NADH	UDP + NADPH		Arabidopsis thaliana	-	ATP is preferred substrate	657853	-	-	?
ITP + NADH	IDP + NADPH		Saccharomyces cerevisiae	-	61% of activity with ATP	642062	-	-	?
additional information	?	-	Saccharomyces cerevisiae	-	POS5 NADH kinase is required for mitochondrial stability with a critical role in detoxification of reactive oxygen species	649040	-	-	-
additional information	?	-	Arabidopsis thaliana	-	diphosphate or polyphosphate is not used as a phosphoryl donor, no substrate: diphosphate, polyphosphate	657853	-	-	-
additional information	?	-	Saccharomyces cerevisiae	P32622	NADH kinase does not increase the rate of xylose consumption	695829	-	-	-
additional information	?	-	Saccharomyces cerevisiae	C7GJD6	does not accept poly(P) as phosphoryl donor	691390	-	-	-
additional information	?	-	Fusarium oxysporum	-	the purified enzyme presents almost no activity against NAD+	706568	-	-	-

NATURAL SUBSTRATES	NATURAL PRODUCTS	REACTION DIAGRAM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY SUBSTRATE	LITERATURE (Substrate)	COMMENTARY PRODUCT	LITERATURE (Product)
ATP + NAD+	ADP + NADP+		Emericella nidulans	-	-	693998	-	-
ATP + NADH	ADP + NADPH		Saccharomyces cerevisiae	-	may be involved in maintaining the level of triphosphopyridine nucleotide in the mitochondria	642062	-	642062
ATP + NADH	ADP + NADPH		Saccharomyces cerevisiae	-	the enzyme has primarily NADH kinase activity in mitochondria, mitochondrial NADPH is largely provided by the POS5 NADH kinase	649041	-	649041
ATP + NADH	ADP + NADPH		Saccharomyces cerevisiae	Q06892	Pos5 is responsible for all mitochondrial NAD/NADH kinase. Transitory exposure of Pos5 to the cytoplasm is required for the viability of utr1 mutants	674616	-	-
additional information	?	-	Saccharomyces cerevisiae	-	POS5 NADH kinase is required for mitochondrial stability with a critical role in detoxification of reactive oxygen species	649040	-	-
additional information	?	-	Saccharomyces cerevisiae	P32622	NADH kinase does not increase the rate of xylose consumption	695829	-	-

COFACTOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
ATP	Micrococcus flavus, Micrococcus flavus AKU 0502, Micrococcus flavus ICR 1820, Mycobacterium tuberculosis	-	-	662376	2D-image
ATP	Saccharomyces cerevisiae	-	-	661696, 674616, 695829, 705593	2D-image
ATP	Arabidopsis thaliana	-	-	676008, 676516, 706395	2D-image
ATP	Fusarium oxysporum	-	-	706568	2D-image
NAD+	Saccharomyces cerevisiae	P32622	;	695829	2D-image
NADH	Arabidopsis thaliana	-	-	691390	2D-image
NADH	Saccharomyces cerevisiae	C7GJD6	-	691390	2D-image

METALS and IONS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
Ca2+	Arabidopsis thaliana	-	8% of the activity with Mg2+; far less active than Mg2+ and Mn2+	657853
Ca2+	Saccharomyces cerevisiae	-	26% activity in the presence of 1mM Ca2+	661696
Co2+	Saccharomyces cerevisiae	-	32% activity in the presence of 1mM Co2+	661696
K+	Fusarium oxysporum	-	K+ is the only monovalent metal ion resulting higher NADH kinase activity by almost 1.5fold	706568
Mg2+	Saccharomyces cerevisiae	-	either Mn2+ or Mg2+ activate at low concentrations. Km: Km: 1.0 mM	642062
Mg2+	Arabidopsis thaliana	-	Mn2+ can substitute for Mg2+ to stabilize the phosphoanhydride bonds of ATP but with only 28% of the activity. Km-value Mg2+, 1.16 mM; required, Km-value 1.16 mM	657853
Mg2+	Saccharomyces cerevisiae	-	100% activity in the presence of 1mM Mg2+	661696
Mg2+	Arabidopsis thaliana	Q56YN3	required for activity	676008
Mn2+	Saccharomyces cerevisiae	-	either Mn2+ or Mg2+ activate at low concentrations	642062
Mn2+	Arabidopsis thaliana	-	28% of the activity with Mg2+; Mn2+ can substitute for Mg2+ to stabilize the phosphoanhydride bonds of ATP but with only 28% of the activity	657853
Zn2+	Arabidopsis thaliana	-	4% of the activity with Mg2+; far less active than Mg2+ and Mn2+	657853
Mn2+	Saccharomyces cerevisiae	-	77% activity in the presence of 1mM Mn2+	661696
additional information	Saccharomyces cerevisiae	-	no activity is detected in the presence of 1 mm Zn2+, Fe2+, Cu2+, Na+, and Li+	661696
additional information	Arabidopsis thaliana	Q56YN3	no significant increase in NAD(H) kinase activity is detected in the presence of Ca2+ /calmodulin	676008

INHIBITORS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
3-acetyl-NAD+	Saccharomyces cerevisiae	-	1 mM, 15% inhibition	642066	2D-image
Ag2+	Fusarium oxysporum	-	1 mM Ag+ lowers the activity to 3%	706568	2D-image
ATP	Saccharomyces cerevisiae	-	above 5 mM	642062	2D-image
ATP	Saccharomyces cerevisiae	-	above 6 mM	642066	2D-image
beta-mercaptoethanol	Fusarium oxysporum	-	slightly inhibits NADH kinase	706568	2D-image
Ca2+	Fusarium oxysporum	-	no activity in the presence of 10 mM	706568	2D-image
Cu2+	Fusarium oxysporum	-	1 mM Cu2+ lowers the activity to 33%	706568	2D-image
diphosphate	Arabidopsis thaliana	-	mixed inhibitor both to NADH and ATP	657853	2D-image
eosin-5-maleimide	Saccharomyces cerevisiae	-	-	642064	2D-image
Fe2+	Fusarium oxysporum	-	no activity in the presence of 10 mM	706568	2D-image
HgCl2	Saccharomyces cerevisiae	-	13% residual activity at 0.1 mM, 6% residual activity at 0.25 mM	661696	2D-image
iodoacetic acid	Saccharomyces cerevisiae	-	5 mM, 87% inhibition, NADH protects	642066	2D-image
Mg2+	Saccharomyces cerevisiae	-	at 10 mM or above	642062	2D-image
NADH	Saccharomyces cerevisiae	-	slight inhibition	661696	2D-image
NADP+	Fusarium oxysporum	-	slightly inhibits NADH kinase	706568	2D-image
NADPH	Saccharomyces cerevisiae	-	slight inhibition	661696	2D-image
NEM	Saccharomyces cerevisiae	-	5 mM, 77% inhibition, NADH protects, ATP not	642066	2D-image
p-chloromercuribenzoate	Arabidopsis thaliana	-	0.01 mM, 42% residual activity	657853	2D-image
PCMB	Saccharomyces cerevisiae	-	-	642064	2D-image
PCMB	Saccharomyces cerevisiae	-	5 mM, 49% inhibition	642066	2D-image
phosphoenolpyruvate	Saccharomyces cerevisiae	-	20 mM, 63% inhibition, reconstituted enzyme. Solubilized enzyme is inhibited 55% by 30 mM	642065	2D-image
phosphoenolpyruvate	Saccharomyces cerevisiae	-	30 mM, 57% inhibition	642066	2D-image
Zn2+	Fusarium oxysporum	-	1 mM Zn2+ lowers the activity to 35%	706568	2D-image
Mn2+	Saccharomyces cerevisiae	-	at 1 mM or above	642062	2D-image
additional information	Fusarium oxysporum	-	NAD+ causes no inhibition of the enzyme	706568	-

ACTIVATING COMPOUND	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
acetate	Saccharomyces cerevisiae	-	high concentrations of carboxylic acid, e.g. acetate required for maximal activity	642062	2D-image
acetate	Saccharomyces cerevisiae	-	maximal enhancement of activity, about 7fold is observed with 100 mM acetate, enzyme reconstituted in liposomes, maximal enhancement of solubilized enzyme with 400 mM acetate	642065	2D-image
acetate	Saccharomyces cerevisiae	-	activates	642066	2D-image
EGTA	Arabidopsis thaliana	Q56YN3	0.5 mM	676008	2D-image

KM VALUE [mM]	KM VALUE [mM] Maximum	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
0.062	-	ATP	Arabidopsis thaliana	-	pH 7.9, 25�C; pH 7.9, 25�C, cosubstrate NADH	657853	2D-image
0.133	-	ATP	Saccharomyces cerevisiae	-	NADH kinase reconstituted in liposomes	642065	2D-image
0.32	-	ATP	Micrococcus flavus	-	wild type enzyme	662376	2D-image
0.47	-	ATP	Micrococcus flavus	-	mutant enzyme G183R	662376	2D-image
1	-	ATP	Saccharomyces cerevisiae	-	pH 7.8, 30�C, in presence of 0.2 M sodium acetate	642062	2D-image
2.1	-	ATP	Saccharomyces cerevisiae	-	solubilized enzyme form	642065	2D-image
2.1	-	ATP	Saccharomyces cerevisiae	-	pH 7.4, 30�C	642066	2D-image
2.59	-	ATP	Fusarium oxysporum	-	-	706568	2D-image
0.759	-	CTP	Arabidopsis thaliana	-	pH 7.9, 25�C, cosubstrate NADH	657853	2D-image
0.461	-	GTP	Arabidopsis thaliana	-	pH 7.9, 25�C, cosubstrate NADH	657853	2D-image
2.39	-	NAD+	Arabidopsis thaliana	-	pH 7.9, 25�C; pH 7.9, 25�C, the specificity constant (vmax/Km) for NADH is 100fold greater than for NAD+	657853	2D-image
0.027	-	NADH	Saccharomyces cerevisiae	-	NADH kinase reconstituted in liposomes	642065	2D-image
0.042	-	NADH	Saccharomyces cerevisiae	-	pH 7.8, 30�C, in presence of 0.2 M sodium acetate	642062	2D-image
0.042	-	NADH	Arabidopsis thaliana	-	pH 7.9, 25�C; pH 7.9, 25�C, the specificity constant (vmax/Km) for NADH is 100fold greater than for NAD+	657853	2D-image
0.105	-	NADH	Saccharomyces cerevisiae	-	solubilized enzyme form	642065	2D-image
0.105	-	NADH	Saccharomyces cerevisiae	-	pH 7.4, 30�C	642066	2D-image
0.13	-	NADH	Fusarium oxysporum	-	-	706568	2D-image
0.35	-	NADH	Micrococcus flavus	-	with tetrapolyphosphate as the phosphate donor, wild type enzyme	662376	2D-image
0.45	-	NADH	Micrococcus flavus	-	with ATP as the phosphate donor, wild type enzyme	662376	2D-image
0.72	-	NADH	Micrococcus flavus	-	with tetrapolyphosphate as the phosphate donor, mutant enzyme G183R	662376	2D-image
1.66	-	NADH	Micrococcus flavus	-	with ATP as the phosphate donor, mutant enzyme G183R	662376	2D-image
2	-	NADH	Saccharomyces cerevisiae	-	in 100 mM Tris-HCl (pH 8.0) at 30�C	661696	2D-image
0.58	-	Tetrapolyphosphate	Micrococcus flavus	-	wild type enzyme	662376	2D-image
0.68	-	Tetrapolyphosphate	Micrococcus flavus	-	mutant enzyme G183R	662376	2D-image
0.156	-	UTP	Arabidopsis thaliana	-	pH 7.9, 25�C, cosubstrate NADH	657853	2D-image

TURNOVER NUMBER [1/s]	TURNOVER NUMBER MAXIMUM[1/s]	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
0.84	-	ATP	Fusarium oxysporum	-	-	706568	2D-image
0.95	-	NADH	Fusarium oxysporum	-	-	706568	2D-image

kcat/KM VALUE [1/mMs^-1]	kcat/KM VALUE [1/mMs^-1] Maximum	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

Ki VALUE [mM]	Ki VALUE [mM] Maximum	INHIBITOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

IC50 VALUE [mM]	IC50 VALUE [mM] Maximum	INHIBITOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg]	SPECIFIC ACTIVITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
0.078	-	Saccharomyces cerevisiae	-	cell extract, at 30�C	661696
0.1	-	Saccharomyces cerevisiae	-	with GTP as phosphate donor	674616
0.22	-	Mycobacterium tuberculosis	-	in the presence of 5 mM tetrapolyphosphate	662376
0.279	-	Saccharomyces cerevisiae	-	-	642062
0.5	-	Saccharomyces cerevisiae	-	with CTP as phosphate donor	674616
0.65	-	Mycobacterium tuberculosis	-	in the presence of 5 mM ATP	662376
1.2	-	Saccharomyces cerevisiae	-	with ATP as phosphate donor	674616
3.04	-	Micrococcus flavus	-	in the presence of 5 mM tetrapolyphosphate	662376
3.4	-	Arabidopsis thaliana	Q56YN3	in the presence of 0.5 mM EGTA, using NAD+ as a substrate	676008
5.75	-	Arabidopsis thaliana	Q56YN3	in the presence of 0.5 mM EGTA, using NADH as a substrate	676008
8.66	-	Micrococcus flavus	-	in the presence of 5 mM ATP	662376
9.475	-	Saccharomyces cerevisiae	-	after 122fold purification, at 30�C	661696
15.1	-	Arabidopsis thaliana	-	NAD+ as substrate, pH 7.9, 25�C	657853
15.6	-	Arabidopsis thaliana	-	pH 7.9, 25�C, substrate NAD+	657853
40.1	-	Arabidopsis thaliana	-	NADH as susbtrate, pH 7.9, 25�C; pH 7.9, 25�C, substrate NADH	657853
729	-	Saccharomyces cerevisiae	-	-	642066
additional information	-	Pichia membranifaciens, Pichia membranifaciens H 112	-	amplification of NADH without the influence of coexisting NAD+, by phosphorylating NADH into NADPH and putting this NAPH through an NADP+-NADPH cycling system	642063
additional information	-	Emericella nidulans	-	gene encoding NADH kinase identified, overexpression using constitutive promoter, physiologcal effects in combination with different aeration rates in glucose batch fermentation, metabolite profiling, relative amounts of intracellular amino and non-amino organic acids compared, enzyme activity significantly correlated with growth activity	693998
additional information	-	Fusarium oxysporum	-	the crude extract shows a specific activity of 0.017 units/mg, the 65.62fold purified enzyme shows a specific activity of 1.11 units/mg	706568

pH OPTIMUM	pH MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
7.5	-	Fusarium oxysporum	-	-	706568
7.9	8.6	Saccharomyces cerevisiae	-	-	642062
7.9	-	Arabidopsis thaliana	-	-	657853
8	-	Saccharomyces cerevisiae	-	NADH kinase reconstituted in liposomes	642065
8.5	-	Saccharomyces cerevisiae	-	-	642066, 661696

pH RANGE	pH RANGE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
6.2	9.9	Saccharomyces cerevisiae	-	pH 6.2: about 35% of maximal activity, pH 9.9: about 50% of maximal activity	642062
6.5	8.5	Fusarium oxysporum	-	less than 30% of the peak activity is displayed in pH 6.5 and 8.5	706568

TEMPERATURE OPTIMUM	TEMPERATURE OPTIMUM MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
45	-	Saccharomyces cerevisiae	-	-	661696
45	-	Fusarium oxysporum	-	-	706568

TEMPERATURE RANGE	TEMPERATURE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

pI VALUE	pI VALUE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
5.46	-	Saccharomyces cerevisiae	-	calculated from amino acid sequence	661696
5.6	-	Fusarium oxysporum	-	isoelectric focusing	706568

SOURCE TISSUE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	SOURCE
cell culture	Arabidopsis thaliana	-	-	657853
cell suspension culture	Arabidopsis thaliana	-	-	657853
flower	Arabidopsis thaliana	-	-	676516
leaf	Arabidopsis thaliana	-	-	657853, 676008, 676516
leaf	Arabidopsis thaliana	-	restricted primarily to leaf vasculature	706395
root	Arabidopsis thaliana	-	-	676516
root primordium	Arabidopsis thaliana	-	restricted to lateral root primordium	706395
silique	Arabidopsis thaliana	-	-	676516
stem	Arabidopsis thaliana	-	-	676516
vascular bundle	Arabidopsis thaliana	-	restricted primarily to leaf vasculature	706395

LOCALIZATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	GeneOntology No.	LITERATURE	SOURCE
cytoplasm	Saccharomyces cerevisiae	-	-	5737	674616
cytoplasm	Arabidopsis thaliana	-	-	5737	676516
cytosol	Saccharomyces cerevisiae	-	-	5829	642062
cytosol	Arabidopsis thaliana	-	putative	5829	657853
cytosol	Saccharomyces cerevisiae	P32622	;	5829	695829
mitochondrion	Pichia membranifaciens, Pichia membranifaciens H 112	-	-	5739	642063
mitochondrion	Saccharomyces cerevisiae	-	the enzyme is localized and functions at the intermembrane space side of the inner membrane	5739	642064
mitochondrion	Saccharomyces cerevisiae	-	localized exclusively in mitochondria	5739	642066
mitochondrion	Saccharomyces cerevisiae	-	enzyme functions exclusively in mitochondria	5739	649040
peroxisome	Arabidopsis thaliana	-	NADK3 localizes to the peroxisomal matrix via a novel type 1 peroxisomal targeting signal	5777	706395
mitochondrion	Saccharomyces cerevisiae	-	-	5739	642062, 649041, 674616, 691390, 695829, 705593
additional information	Arabidopsis thaliana	-	not: mitochondrial	-	657853

PDB	SCOP	CATH	ORGANISM
3afo, download	SCOP (3afo)	CATH (3afo)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)

MOLECULAR WEIGHT	MOLECULAR WEIGHT MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
46000	-	Saccharomyces cerevisiae	C7GJD6	-	691390
49000	-	Emericella nidulans	-	predicted	693998
55900	-	Saccharomyces cerevisiae	-	calculated from amino acid sequence	661696
58000	-	Arabidopsis thaliana	Q56YN3	SDS-PAGE	676008
58000	-	Arabidopsis thaliana	-	isoform NADK1	691390
58250	-	Arabidopsis thaliana	Q56YN3	calculated from amino acid sequence	676008
78000	-	Arabidopsis thaliana	-	gel filtration	657853
156000	-	Fusarium oxysporum	-	gel filtration	706568
160000	-	Saccharomyces cerevisiae	-	gel filtration	642066
480000	-	Saccharomyces cerevisiae	-	gel filtration	661696

SUBUNITS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
dimer	Arabidopsis thaliana	-	2 * 35000, SDS-PAGE	657853
homodimer	Micrococcus flavus, Micrococcus flavus AKU 0502, Micrococcus flavus ICR 1820	-	-	662376
homodimer	Fusarium oxysporum	-	2 * 72000, gel filtration	706568
homooctamer	Saccharomyces cerevisiae	-	8 * 60000, gel filtration	661696
additional information	Saccharomyces cerevisiae	-	presence of 2 or more subunits of different size	642066

POSTTRANSLATIONAL MODIFICATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

Crystallization/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

pH STABILITY	pH STABILITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
5.5	-	Fusarium oxysporum	-	the enzyme is very unstable at pH values higher than 5.5	706568

TEMPERATURE STABILITY	TEMPERATURE STABILITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
30	-	Pichia membranifaciens, Pichia membranifaciens H 112	-	10 min, stable	642063
35	-	Pichia membranifaciens, Pichia membranifaciens H 112	-	10 min, 17% loss of activity	642063
35	-	Saccharomyces cerevisiae	-	5 min, 35% loss of activity	642066
40	-	Pichia membranifaciens, Pichia membranifaciens H 112	-	10 min, 40% loss of activity	642063
50	-	Fusarium oxysporum	-	the enzyme retains 8% of the activity after 1 h at 50�C	706568
54	-	Saccharomyces cerevisiae	-	half of its activity is lost on treatment at 54�C for 10 min	661696
55	-	Saccharomyces cerevisiae	-	5 min, 95% loss of activity	642066

GENERAL STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
ammonium sulfate, 0.2 M protects against denaturation	Saccharomyces cerevisiae	-	642062

ORGANIC SOLVENT	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

OXIDATION STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

STORAGE STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
-30�C, 20 mM Tris-HCl buffer, pH 7.8, 0.2 M ammonium sulfate, 20 mM MgCl2, 2 mM EDTA, stable for 1 month or more	Saccharomyces cerevisiae	-	642066
0-5�C, 20 mM Tris-HCl buffer, pH 7.8, 0.2 M ammonium sulfate, 20 mM MgCl2, 2 mM EDTA, stable for 7 days	Saccharomyces cerevisiae	-	642066
4�C or -20�C, 95% loss of activity after overnight storage	Saccharomyces cerevisiae	-	642062

Purification/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
-	Arabidopsis thaliana	-	657853
HiTrap Chelating HP column chromatography	Arabidopsis thaliana	Q56YN3	676008
Q-Sepharose column chromatography, Sephacryl S300 gel filtration, SP-Sepharose column chromatography, and t-butyl hydrophobic interaction column chromatography	Fusarium oxysporum	-	706568
Butyl-Toyopearl 650M column chromatography	Micrococcus flavus, Micrococcus flavus AKU 0502, Micrococcus flavus ICR 1820, Mycobacterium tuberculosis	-	662376
-	Saccharomyces cerevisiae	-	642062, 642066, 649040
; cobalt chelate affinity column chromatography	Saccharomyces cerevisiae	-	674616
DEAE-Toyopearl column chromatography, Butyl-Toyopearl column chromatography, Ni-chelate AF Toyopearl column chromatography	Saccharomyces cerevisiae	-	661696

Cloned/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
a NADK3-GFP reporter fusion construct is expressed in Arabidopsis suspension-cultured cells	Arabidopsis thaliana	-	706395
expressed in Escherichia coli	Arabidopsis thaliana	Q56YN3	676008
expression in Escherichia coli	Arabidopsis thaliana	-	657853
overexpressed in Aspergillus AR1 derived from the natural isolate A4, pTr04 expression vector	Emericella nidulans	-	693998
expressed in Escherichia coli BL21(DE3)pLysS cells	Micrococcus flavus, Micrococcus flavus AKU 0502, Micrococcus flavus ICR 1820, Mycobacterium tuberculosis	-	662376
expressed in Escherichia coli strain MK746 as a fusion recombinant protein with a V5 epitope and His6 tag at the C terminus	Saccharomyces cerevisiae	-	661696
expressed in Escherichia coli; expression in Escherichia coli as N-terminally His-tagged fusion	Saccharomyces cerevisiae	-	674616
overexpression in Escherichia coli	Saccharomyces cerevisiae	-	649041
overproduction of the POS5 gene product in Escherichia coli	Saccharomyces cerevisiae	-	649040

EXPRESSION	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
transcription of isoform NADK1 is upregulated by 3 and 8fold after treatment of plant cells with 5 mM H2O2 and irradiation, respectively	Arabidopsis thaliana	-	691390
NADK3 activity is first observed in stage 1.0-1.02, most notably in the procambium and vasculature of cotyledons	Arabidopsis thaliana	-	706395
transcription of POS5 is upregulated 3.4fold after treatment for 10-12 min with 2.5 mM Cu2+	Saccharomyces cerevisiae	C7GJD6	691390

ENGINEERING	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
G183R	Micrococcus flavus, Micrococcus flavus AKU 0502, Micrococcus flavus ICR 1820	-	mutant with partially retained NADH kinase activity	662376

Renatured/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

APPLICATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
analysis	Pichia membranifaciens, Pichia membranifaciens H 112	-	the enzyme is useful for amplification of NADH in presence of excess NAD+ and is applicable to sensitive measurement of NAD+ dependent dehydrogenase or ist substrate	642063

REF.	AUTHORS	TITLE	JOURNAL	VOL.	PAGES	YEAR	ORGANISM (UNIPROT ACCESSION NO.)	LINK TO PUBMED	SOURCE
642062	Griffiths, M.M.; Bernofsky, C.	Purification and properties of reduced diphosphopyridine nucleotide kinase from yeast mitochondria	J. Biol. Chem.	247	1473-1478	1972	Saccharomyces cerevisiae	PubMed
642063	Ohno, T.; Suzuki, T.; Horiuchi, T.	Specific amplification of NADH using NADH kinase in a reaction mixture containing excess NAD+	Biosci. Biotechnol. Biochem.	58	976-977	1994	Pichia membranifaciens	-
642064	Iwahashi, Y.; Nakamura, T.	Localization of the NADH kinase in the inner membrane of yeast mitochondria	J. Biochem.	105	916-921	1989	Saccharomyces cerevisiae, Saccharomyces cerevisiae X2181-1A	PubMed
642065	Iwahashi, Y.; Nakamura, T.	Orientation and reactivity of NADH kinase in proteoliposomes	J. Biochem.	105	922-926	1989	Saccharomyces cerevisiae, Saccharomyces cerevisiae X2181-1A	PubMed
642066	Iwahashi, Y.; Hitoshio, A.; Tajima, N.; Nakamura, T.	Characterization of NADH kinase from Saccharomyces cerevisiae	J. Biochem.	105	588-593	1989	Saccharomyces cerevisiae, Saccharomyces cerevisiae X2181-1A	PubMed
649040	Strand, M.K.; Stuart, G.R.; Longley, M.J.; Graziewicz, M.A.; Dominick, O.C.; Copeland, W.C.	POS5 gene of Saccharomyces cerevisiae encodes a mitochondrial NADH kinase required for stability of mitochondrial DNA	Eukaryot. Cell	2	809-820	2003	Saccharomyces cerevisiae	PubMed
649041	Outten, C.E.; Culotta, V.C.	A novel NADH kinase is the mitochondrial source of NADPH in Saccharomyces cerevisiae	EMBO J.	22	2015-2024	2003	Saccharomyces cerevisiae	PubMed
657853	Turner, W.L.; Waller, J.C.; Snedden, W.A.	Identification, molecular cloning and functional characterization of a novel NADH kinase from Arabidopsis thaliana (thale cress)	Biochem. J.	385	217-223	2005	Arabidopsis thaliana	PubMed
661696	Shi, F.; Kawai, S.; Mori, S.; Kono, E.; Murata, K.	Identification of ATP-NADH kinase isozymes and their contribution to supply of NADP(H) in Saccharomyces cerevisiae	FEBS J.	272	3337-3349	2005	Saccharomyces cerevisiae	PubMed
662376	Mori, S.; Kawai, S.; Shi, F.; Mikami, B.; Murata, K.	Molecular conversion of NAD Kinase to NADH kinase through single amino acid residue substitution	J. Biol. Chem.	280	24104-24112	2005	Micrococcus flavus, Mycobacterium tuberculosis	PubMed
674616	Bieganowski, P.; Seidle, H.F.; Wojcik, M.; Brenner, C.	Synthetic lethal and biochemical analyses of NAD and NADH kinases in Saccharomyces cerevisiae establish separation of cellular functions	J. Biol. Chem.	281	22439-22445	2006	Saccharomyces cerevisiae (P21373), Saccharomyces cerevisiae (P32622), Saccharomyces cerevisiae, Saccharomyces cerevisiae (Q06892)	PubMed
676008	Berrin, J.; Brutesco, C.; Montillet, J.; Kazmaier, M.; Pierrugues, O.; Alonso, B.; Roby, D.	Stress induces the expression of AtNADK-1, a gene encoding a NAD(H) kinase in Arabidopsis thaliana	Mol. Genet. Genomics	273	10-19	2005	Arabidopsis thaliana, Arabidopsis thaliana (Q56YN3)	PubMed
676516	Chai, M.F.; Wei, P.C.; Chen, Q.J.; An, R.; Chen, J.; Yang, S.; Wang, X.C.	NADK3, a novel cytoplasmic source of NADPH, is required under conditions of oxidative stress and modulates abscisic acid responses in Arabidopsis	Plant J.	47	665-674	2006	Arabidopsis thaliana	PubMed
691390	Kawai, S.; Murata, K.	Structure and function of NAD kinase and NADP phosphatase: key enzymes that regulate the intracellular balance of NAD(H) and NADP(H)	Biosci. Biotechnol. Biochem.	72	919-930	2008	Arabidopsis thaliana, Arabidopsis thaliana (Q500Y9), Arabidopsis thaliana (Q56YN3), Arabidopsis thaliana (Q9C5W3), Saccharomyces cerevisiae (C7GKE4), Saccharomyces cerevisiae (P21373), Saccharomyces cerevisiae (P32622), Saccharomyces cerevisiae (Q06892), Saccharomyces cerevisiae, Saccharomyces cerevisiae (C7GJD6)	PubMed
693998	Panagiotou, G.; Grotkjaer, T.; Hofmann, G.; Bapat, P.M.; Olsson, L.	Overexpression of a novel endogenous NADH kinase in Aspergillus nidulans enhances growth	Metab. Eng.	11	31-39	2008	Emericella nidulans, Emericella nidulans CBS 513.88 /FGSC A1513	PubMed
695829	Hou, J.; Vemuri, G.N.; Bao, X.; Olsson, L.	Impact of overexpressing NADH kinase on glucose and xylose metabolism in recombinant xylose-utilizing Saccharomyces cerevisiae	Appl. Microbiol. Biotechnol.	82	909-919	2009	Saccharomyces cerevisiae, Saccharomyces cerevisiae (P32622), Saccharomyces cerevisiae (Q06892)	PubMed
705593	Stuart, G.R.; Humble, M.M.; Strand, M.K.; Copeland, W.C.	Transcriptional response to mitochondrial NADH kinase deficiency in Saccharomyces cerevisiae	Mitochondrion	9	211-221	2009	Saccharomyces cerevisiae, Saccharomyces cerevisiae YPH925	PubMed
706395	Waller, J.C.; Dhanoa, P.K.; Schumann, U.; Mullen, R.T.; Snedden, W.A.	Subcellular and tissue localization of NAD kinases from Arabidopsis: compartmentalization of de novo NADP biosynthesis	Planta	231	305-317	2010	Arabidopsis thaliana	PubMed
706568	Panagiotou, G.; Papadakis, M.; Topakas, E.; Olsson, L.; Christakopoulos, P.	Identification of NADH kinase activity in filamentous fungi and structural modelling of the novel enzyme from Fusarium oxysporum	Process Biochem.	43	1114-1120	2008	Fusarium oxysporum, Fusarium oxysporum F3	-

LINKS TO OTHER DATABASES (specific for EC-Number 2.7.1.86)

NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM

IUBMB Enzyme Nomenclature

PROSITE Database of protein families and domains

SYSTERS

Protein Mutant Database

InterPro (database of protein families, domains and functional sites)

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