Information on EC 2.7.1.76 - deoxyadenosine kinase:
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EC NUMBERCOMMENTARY
2.7.1.76-

RECOMMENDED NAMEGeneOntology No.
deoxyadenosine kinaseGO:0004136

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
ATP + deoxyadenosine = ADP + dAMP
show the reaction diagram		----
ATP + deoxyadenosine = ADP + dAMP
show the reaction diagram		-Bacillus subtilis-640561
ATP + deoxyadenosine = ADP + dAMP
show the reaction diagram		random sequential substrate additionLactobacillus acidophilus-641950

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
phospho group transfer----

PATHWAYKEGG LinkMetaCyc Link
No entries in this field

SYSTEMATIC NAMEIUBMB Comments
ATP:deoxyadenosine 5'-phosphotransferaseDeoxyguanosine can also act as acceptor. Possibly identical with EC 2.7.1.74 deoxycytidine kinase.

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
dAdo/dCyd kinaseBacillus subtilis--640561
dAKMycoplasma mycoides--672159, 675428
dAKBacillus cereusQ0H0H5-674219
dAKDictyostelium discoideumQ54YL2-675444
dCKHomo sapiens--660855, 662985
dCyd kinase/dAdo kinase ILactobacillus acidophilus--640566
deoxycytidine kinase/deoxyadenosine kinase, dCK/dAKLactobacillus acidophilusP0C1F9-640567
deoxyguanosine kinase/deoxyadenosine kinase, dGK/dAKLactobacillus acidophilusP0C1F9-640567
dGuo kinase/dAdo kinase IILactobacillus acidophilus--640566
purine-deoxyribonucleoside kinase----
kinase, deoxyadenosine (phosphorylating)----
additional informationRattus norvegicus-enzyme may be identical with EC 2.7.1.20, i.e. adenosine kinase641089
additional informationBos taurus-may be identical with EC 2.7.1.113, i.e. deoxyguanosine kinase641974
additional informationHomo sapiens-enzyme may be identical with EC 2.7.1.20, i.e. adenosine kinase641987

CAS REGISTRY NUMBERCOMMENTARY
37278-12-9-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Acholeplasma laidlawii-641988--Manually annotated by BRENDA team
Bacillus cereus-674219Q0H0H5TrEMBLManually annotated by BRENDA team
Bacillus subtilisgene yaaF, yaaF encodes an enzyme with specificity for deoxyadenosine and deoxycytidine640561--Manually annotated by BRENDA team
Bos taurus-641977, 641981--Manually annotated by BRENDA team
Bos tauruscalf641974--Manually annotated by BRENDA team
Dictyostelium discoideum-675444--Manually annotated by BRENDA team
Dictyostelium discoideum-675444Q54YL2TrEMBLManually annotated by BRENDA team
Homo sapiens-641937, 641987, 660855, 662985, 694483--Manually annotated by BRENDA team
Homo sapiensnewborn641099--Manually annotated by BRENDA team
Lactobacillus acidophilus-640565, 641989P0C1F9SwissProtManually annotated by BRENDA team
Lactobacillus acidophilus-641950, 641980--Manually annotated by BRENDA team
Lactobacillus acidophilusstrain R-26640566--Manually annotated by BRENDA team
Lactobacillus acidophilusstrain R-26640567P0C1F9SwissProtManually annotated by BRENDA team
Mus musculus-641984--Manually annotated by BRENDA team
Mycoplasma arginini-641988--Manually annotated by BRENDA team
Mycoplasma mycoides-675428Q93IG4UniProtManually annotated by BRENDA team
Mycoplasma mycoidessubspecies mycoides SC641988--Manually annotated by BRENDA team
Mycoplasma mycoidessubspecies Mycoplasma mycoides mycoides675428--Manually annotated by BRENDA team
Mycoplasma mycoidessubspecies Mycoplasma mycoides mycoides strain SC672159--Manually annotated by BRENDA team
Mycoplasma pneumoniae-641988--Manually annotated by BRENDA team
Rattus norvegicus-641089, 641976--Manually annotated by BRENDA team
Rhodobacter sphaeroides R-26strain R-26640567P0C1F9SwissProtManually annotated by BRENDA team
Solanum tuberosum-676722--Manually annotated by BRENDA team
Staphylococcus aureus-690459Q0H0H0UniProtManually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
2'-deoxyadenosine-3'-fluoro-3'-triphosphate + 2'-deoxyadenosine2'-deoxyadenosine-3'-fluoro-3'-diphosphate + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram		Homo sapiens--662985--?
2'-deoxyadenosine-3'-fluoro-3'-triphosphate + 2'-deoxycytidine2'-deoxyadenosine-3'-fluoro-3'-diphosphate + 2'-deoxycytidine 5'-phosphate
show the reaction diagram		Homo sapiens--662985--?
2'-deoxyadenosine-3'-fluoroara-3'-triphosphate + 2'-deoxyadenosine2'-deoxyadenosine-3'-fluoroara-3'-diphosphate + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram		Homo sapiens-low activity662985--?
2'-deoxyadenosine-3'-fluoroara-3'-triphosphate + 2'-deoxycytidine2'-deoxyadenosine-3'-fluoroara-3'-diphosphate + 2'-deoxycytidine 5'-phosphate
show the reaction diagram		Homo sapiens-low activity662985--?
2'-deoxyadenosine-3'-triphosphate + 2'-deoxyadenosine2'-deoxyadenosine-3'-diphosphate + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram		Homo sapiens--662985--?
2'-deoxyadenosine-3'-triphosphate + 2'-deoxycytidine2'-deoxyadenosine-3'-diphosphate + 2'-deoxycytidine 5'-phosphate
show the reaction diagram		Homo sapiens--662985--?
3'-deoxyadenosine 2'-triphosphate + 2'-deoxyadenosine3'-deoxyadenosine-2'-diphosphate + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram		Homo sapiens--662985--?
3'-deoxyadenosine 2'-triphosphate + 2'-deoxycytidine3'-deoxyadenosine-2'-diphosphate + 2'-deoxycytidine 5'-phosphate
show the reaction diagram		Homo sapiens--662985--?
3'-deoxyadenosine-3'-fluoro-2'-triphosphate + 2'-deoxyadenosine3'-deoxyadenosine-3'-fluoro-2'-diphosphate + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram		Homo sapiens--662985--?
3'-deoxyadenosine-3'-fluoro-2'-triphosphate + 2'-deoxycytidine3'-deoxyadenosine-3'-fluoro-2'-diphosphate + 2'-deoxycytidine 5'-phosphate
show the reaction diagram		Homo sapiens--662985--?
3'-deoxyadenosine-3'-fluoroxylo-2'-triphosphate + 2'-deoxyadenosine3'-deoxyadenosine-3'-fluoroxylo-2'-diphosphate + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram		Homo sapiens--662985--?
3'-deoxyadenosine-3'-fluoroxylo-2'-triphosphate + 2'-deoxycytidine3'-deoxyadenosine-3'-fluoroxylo-2'-diphosphate + 2'-deoxycytidine 5'-phosphate
show the reaction diagram		Homo sapiens--662985--?
ATP + 1-beta-D-arabinofuranosyl cytosineADP + 1-beta-D-arabinofuranosyl cytosine 5'-phosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG43% relative activity compared to deoxyadenosine675428--?
ATP + 1-beta-D-arabinofuranosyl cytosineADP + 1-beta-D-arabinofuranosyl cytosine 5'-phosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG43% of the activity with 2'-deoxyadenosine675428--?
ATP + 1-beta-D-arabinofuranosyl uracilADP + 1-beta-D-arabinofuranosyl uracil 5'-phosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG40.3% of the activity with 2'-deoxyadenosine675428--?
ATP + 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamideADP + 1-beta-D-ribofuranosyl-1,2,4-triazole-3-carboxamide 5'-monophosphate
show the reaction diagram		Rattus norvegicus-trivial name ribavirin641976-641976?
ATP + 2',2'-difluoro-2'-deoxycytidineADP + 2',2'-difluoro-2'-deoxycytidine monophosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG420% relative activity compared to deoxyadenosine675428--?
ATP + 2',2'-difluoro-2'-deoxycytidineADP + 2',2'-difluoro-2'-deoxycytidine 5'-phosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG420% of the activity with 2'-deoxyadenosine675428--?
ATP + 2',2'-difluoro-2'-deoxyguanosineADP + 2',2'-difluoro-2'-deoxyguanosine monophosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG424% relative activity compared to deoxyadenosine675428--?
ATP + 2',2'-difluoro-2'-deoxyguanosineADP + 2',2'-difluoro-2'-deoxyguanosine 5'-phosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG424% of the activity with 2'-deoxyadenosine675428--?
ATP + 2',2'-difluorodeoxycytidineADP + 2',2'-difluorodeoxycytidine 5'-phosphate
show the reaction diagram		Staphylococcus aureusQ0H0H0-690459--?
ATP + 2',2'-difluorodeoxycytidineADP + 2',2'-difluorodeoxycytidine 5'-phosphate
show the reaction diagram		Bacillus cereusQ0H0H5enzyme activates 2',2'-difluorodesoxycytidine in vivo674219--?
ATP + 2',3'-dideoxyadenosineADP + 2',3'-dideoxyadenosine monophosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG41.5% relative activity compared to deoxyadenosine675428--?
ATP + 2',3'-dideoxyadenosineADP + 2',3'-dideoxyadenosine 5'-monophosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG41.5% of the activity with 2'-deoxyadenosine675428--?
ATP + 2',3'-dideoxycytidineADP + 2',3'-dideoxycytidine 5'-monophosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG40.1% of the activity with 2'-deoxyadenosine675428--?
ATP + 2',3'-dideoxyguanosineADP + 2',3'-dideoxyguanosine 5'-monophosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG40.5% of the activity with 2'-deoxyadenosine675428--?
ATP + 2',3'-dideoxyinoosineADP + 2',3'-dideoxyinosine 5'-monophosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG43.9% of the activity with 2'-deoxyadenosine675428--?
ATP + 2',3'-dideoxyinosineADP + 2',3'-dideoxyinosine 5'-phosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG43.9% relative activity compared to deoxyadenosine675428--?
ATP + 2'-deoxyadenosineADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram		Homo sapiens--662985--?
ATP + 2'-deoxyadenosineADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram		Homo sapiens--660855--ir
ATP + 2'-deoxyadenosineADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram		Dictyostelium discoideumQ54YL2-675444--?
ATP + 2'-deoxyadenosineADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram		Bacillus cereusQ0H0H5-674219--?
ATP + 2'-deoxyadenosineADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram		Staphylococcus aureusQ0H0H0-690459--?
ATP + 2'-deoxyadenosineADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG4-675428--?
ATP + 2'-deoxycytidineADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram		Homo sapiens--662985--?
ATP + 2'-deoxycytidineADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram		Homo sapiens--660855--ir
ATP + 2'-deoxycytidineADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram		Bacillus cereusQ0H0H5-674219--?
ATP + 2'-deoxycytidineADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram		Staphylococcus aureusQ0H0H0-690459--?
ATP + 2'-deoxycytidineADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG481% of the activity with 2'-deoxyadenosine675428--?
ATP + 2'-deoxyguanosineADP + 2'-deoxyguanosine 5'-phosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG497% of the activity with 2'-deoxyadenosine675428--?
ATP + 2'-deoxyinosineADP + 2'-deoxyinosine 5'-phosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG4116% of the activity with 2'-deoxyadenosine675428--?
ATP + 2'-deoxythymidineADP + 2'-deoxythymidine 5'-phosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG40.01% of the activity with 2'-deoxyadenosine675428--?
ATP + 2'-deoxyuridineADP + 2'-deoxyuridine 5'-phosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG40.9% of the activity with 2'-deoxyadenosine675428--?
ATP + 2'-fluoro-arabinosyl adenineADP + 2'-fluoro-arabinosyl adenine monophosphate
show the reaction diagram		Dictyostelium discoideumQ54YL2i.e. fludarabine675444--?
ATP + 2'-fluoro-arabinosyl adenineADP + 2'-fluoro-arabinosyl adenine monophosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG415% relative activity compared to deoxyadenosine675428--?
ATP + 2'-fluoro-arabinosyl adenineADP + 2'-fluoro-arabinosyl adenine 5'-monophosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG415% of the activity with 2'-deoxyadenosine675428--?
ATP + 2-chloro-2'-deoxyadenosineADP + 2-chloro-2'-deoxyadenosine monophosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG4111% relative activity compared to deoxyadenosine675428--?
ATP + 2-chloro-2'-deoxyadenosineADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG4111% of the activity with 2'-deoxyadenosine675428--?
ATP + 9-beta-D-arabinofuranosyl adenineADP + 9-beta-D-arabinofuranosyl adenine 5'-monophosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG477% relative activity compared to deoxyadenosine675428--?
ATP + 9-beta-D-arabinofuranosyl adenineADP + 9-beta-D-arabinofuranosyl adenine 5'-phosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG477% of the activity with 2'-deoxyadenosine675428--?
ATP + 9-beta-D-arabinofuranosyl guanineADP + 9-beta-D-arabinofuranosyl guanine 5'-monophosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG46% relative activity compared to deoxyadenosine675428--?
ATP + 9-beta-D-arabinofuranosyl guanineADP + 9-beta-D-arabinofuranosyl guanine 5'-phosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG46% of the activity with 2'-deoxyadenosine675428--?
ATP + adenosineADP + AMP
show the reaction diagram		Homo sapiens--641987-641987?
ATP + adenosineADP + AMP
show the reaction diagram		Rattus norvegicus-kinetic data suggest that different catalytic sites of deoxyadenosine kinase or a different enzyme are responsible for deoxyadenosine and adenosine kinase activities641976-641976?
ATP + adenosineADP + AMP
show the reaction diagram		Mycoplasma mycoidesQ93IG47.4% relative activity compared to deoxyadenosine675428--?
ATP + adenosineADP + adenosine 5'-monophosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG47.4% of the activity with 2'-deoxyadenosine675428--?
ATP + cytidineADP + CMP
show the reaction diagram		Bos taurus-approx. 18% of activity with deoxyadenosine641981-641981?
ATP + cytidineADP + cytidine 5'-monophosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG40.1% of the activity with 2'-deoxyadenosine675428--?
ATP + deoxyadenosineADP + dAMP
show the reaction diagram		Mus musculus--641984-641984?
ATP + deoxyadenosineADP + dAMP
show the reaction diagram		Homo sapiens--641937-641937?
ATP + deoxyadenosineADP + dAMP
show the reaction diagram		Rattus norvegicus--641089-641089?
ATP + deoxyadenosineADP + dAMP
show the reaction diagram		Bos taurus--641977-641977?
ATP + deoxyadenosineADP + dAMP
show the reaction diagram		Bos taurus--641981-641981?
ATP + deoxyadenosineADP + dAMP
show the reaction diagram		Solanum tuberosum--676722--?
ATP + deoxyadenosineADP + dAMP
show the reaction diagram		Acholeplasma laidlawii--641988-641988?
ATP + deoxyadenosineADP + dAMP
show the reaction diagram		Lactobacillus acidophilus--641980-641980?
ATP + deoxyadenosineADP + dAMP
show the reaction diagram		Mycoplasma mycoides--641988-641988?
ATP + deoxyadenosineADP + dAMP
show the reaction diagram		Mycoplasma mycoides--672159--?
ATP + deoxyadenosineADP + dAMP
show the reaction diagram		Mycoplasma pneumoniae, Mycoplasma arginini--641988-641988?
ATP + deoxyadenosineADP + dAMP
show the reaction diagram		Lactobacillus acidophilusP0C1F9-640567-640567?
ATP + deoxyadenosineADP + dAMP
show the reaction diagram		Bacillus cereusQ0H0H5-674219--?
ATP + deoxyadenosineADP + dAMP
show the reaction diagram		Dictyostelium discoideumQ54YL2-675444--?
ATP + deoxyadenosineADP + dAMP
show the reaction diagram		Mycoplasma mycoidesQ93IG4100% activity675428--?
ATP + deoxyadenosineADP + dAMP
show the reaction diagram		Bos taurus-transfers a phospho group from specific nucleoside 5'-triphosphate donors to 5'-position of deoxyadenosine, no activity with adenosine and guanosine641974-641974?
ATP + deoxyadenosineADP + dAMP
show the reaction diagram		Rattus norvegicus-no activity with cytidine, uridine, guanosine, deoxyguanosine and thymidine641976-641976?
ATP + deoxyadenosineADP + dAMP
show the reaction diagram		Lactobacillus acidophilus-enzyme bears two separate but interacting active sites for deoxyadenosine and deoxycytidine kinase activity641950-641950?
ATP + deoxyadenosineADP + dAMP
show the reaction diagram		Homo sapiens-enzyme has both adenosine kinase and deoxyadenosine kinase activity641987-641987-
ATP + deoxyadenosineADP + dAMP
show the reaction diagram		Lactobacillus acidophilus-enzyme exists in two heterodimeric complexes, complex I: deoxycytidine/deoxyadenosine kinase and complex II: deoxyguanosine/deoxyadenosine kinase640566-640566?
ATP + deoxyadenosineADP + 5'-dAMP
show the reaction diagram		Homo sapiens-key anabolic enzyme for activation of purine and pyrimidine deoxyribonucleosides as well as cytosine arabinoside and other anti-tumour drugs641937-641937?
ATP + deoxyadenosineADP + 5'-dAMP
show the reaction diagram		Bos taurus-involved in biosynthesis of nucleoside monophosphates from preformed deoxyribonucleosides641977-641977?
ATP + deoxyadenosineADP + 5'-dAMP
show the reaction diagram		Lactobacillus acidophilusP0C1F9dGK/dAK plays an essential role in generating the dexyribonucleotide precursors, dGTP and dATP, for DNA metabolism640567-640567?
ATP + deoxyadenosineADP + 5'-dAMP
show the reaction diagram		Mus musculus-involved in nucleoside metabolism641984-641984?
ATP + deoxycytidineADP + dCMP
show the reaction diagram		Lactobacillus acidophilus--641950-641950?
ATP + deoxycytidineADP + dCMP
show the reaction diagram		Mycoplasma mycoides--641988-641988?
ATP + deoxycytidineADP + dCMP
show the reaction diagram		Mycoplasma mycoides--672159--?
ATP + deoxycytidineADP + dCMP
show the reaction diagram		Bacillus cereusQ0H0H5-674219--?
ATP + deoxycytidineADP + dCMP
show the reaction diagram		Bos taurus-approx. 12% of activity with deoxyadenosine641981-641981?
ATP + deoxycytidineADP + dCMP
show the reaction diagram		Homo sapiens-deoxycytidine kinase is a multisubstrate enzyme that also phosphorylates deoxyadenosine, it exists in different conformational states with different substrate kinetic properties641937-641937?
ATP + deoxycytosineADP + dCMP
show the reaction diagram		Mycoplasma mycoidesQ93IG481% relative activity compared to deoxyadenosine675428--?
ATP + deoxyguanosineADP + dGMP
show the reaction diagram		Bos taurus--641974-641974?
ATP + deoxyguanosineADP + dGMP
show the reaction diagram		Bos taurus--641981-641981?
ATP + deoxyguanosineADP + dGMP
show the reaction diagram		Mycoplasma mycoides--672159--?
ATP + deoxyguanosineADP + dGMP
show the reaction diagram		Mycoplasma mycoides-approx. 40% of activity with deoxyadenosine641988-641988?
ATP + deoxyguanosineADP + dGMP
show the reaction diagram		Mycoplasma mycoidesQ93IG497% relative activity compared to deoxyadenosine675428--?
ATP + deoxyinosineADP + dIMP
show the reaction diagram		Mycoplasma mycoidesQ93IG4116% relative activity compared to deoxyadenosine675428--?
ATP + deoxyuridineADP + dUMP
show the reaction diagram		Mycoplasma mycoidesQ93IG40.9% relative activity compared to deoxyadenosine675428--?
ATP + fludarabineADP + fludarabine monophosphate
show the reaction diagram		Dictyostelium discoideumQ54YL2-675444--?
ATP + gemcitabineADP + ?
show the reaction diagram		Bacillus cereusQ0H0H5-674219--?
ATP + guanosineADP + GMP
show the reaction diagram		Mycoplasma mycoidesQ93IG45.5% relative activity compared to deoxyadenosine675428--?
ATP + guanosineADP + guanosine 5'-monophosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG45.5% of the activity with 2'-deoxyadenosine675428--?
ATP + inosineADP + IMP
show the reaction diagram		Mycoplasma mycoidesQ93IG45.9% relative activity compared to deoxyadenosine675428--?
ATP + inosineADP + inosine monophosphate
show the reaction diagram		Mycoplasma mycoidesQ93IG45.9% of the activity with 2'-deoxyadenosine675428--?
ATP + penciclovirADP + ?
show the reaction diagram		Mycoplasma mycoidesQ93IG41.4% relative activity compared to deoxyadenosine675428--?
ATP + thymidineADP + thymidinee 5'-phosphate
show the reaction diagram		Dictyostelium discoideumQ54YL2-675444--?
CTP + 2'-deoxyadenosineCDP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram		Dictyostelium discoideumQ54YL2CTP, 3% of the activity with ATP675444--?
CTP + deoxyadenosineCDP + dAMP
show the reaction diagram		Mycoplasma mycoides--641988-641988?
CTP + deoxyadenosineCDP + dAMP
show the reaction diagram		Mycoplasma mycoides--672159--?
CTP + deoxyadenosineCDP + dAMP
show the reaction diagram		Mycoplasma mycoidesQ93IG457% activity compared to ATP675428--?
dATP + deoxyadenosinedADP + dAMP
show the reaction diagram		Mycoplasma mycoidesQ93IG42% activity compared to ATP675428--?
dCTP + deoxyadenosinedCDP + dAMP
show the reaction diagram		Mycoplasma mycoidesQ93IG4106% activity compared to ATP675428--?
dTTP + deoxyadenosinedTDP + dAMP
show the reaction diagram		Bos taurus-75% of activity with ATP641981-641981?
dTTP + deoxyadenosinedTDP + dAMP
show the reaction diagram		Mycoplasma mycoidesQ93IG444% activity compared to ATP675428--?
GTP + 2'-deoxyadenosineGDP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram		Bacillus subtilis--640561--?
GTP + 2'-deoxyadenosineGDP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram		Dictyostelium discoideumQ54YL2GTP, 14% of the activity with ATP675444--?
GTP + 2'-deoxycytidineGDP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram		Bacillus subtilis--640561--?
GTP + deoxyadenosineGDP + dAMP
show the reaction diagram		Bos taurus--641981-641981?
triphosphate + 2'-deoxycytidinediphosphate + 2'-deoxycytidine 5'-phosphate
show the reaction diagram		Homo sapiens--660855--?
TTP + deoxyadenosineTDP + dAMP
show the reaction diagram		Mycoplasma mycoides--641988-641988?
TTP + deoxyadenosineTDP + dAMP
show the reaction diagram		Mycoplasma mycoides--672159--?
UTP + 2'-deoxyadenosineUDP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram		Dictyostelium discoideumQ54YL2UTP, 2% of the activity with ATP675444--?
UTP + deoxyadenosineUDP + dAMP
show the reaction diagram		Mycoplasma mycoides--641988-641988?
UTP + deoxyadenosineUDP + dAMP
show the reaction diagram		Mycoplasma mycoides--672159--?
UTP + deoxyadenosineUDP + dAMP
show the reaction diagram		Bos taurus-90% of activity with ATP641981-641981?
UTP + deoxyadenosineUDP + dAMP
show the reaction diagram		Mycoplasma mycoidesQ93IG422% activity compared to ATP675428--?
GTP + deoxyadenosineGDP + dAMP
show the reaction diagram		Mycoplasma mycoides-10% of activity with ATP641988-641988?
additional information?-Homo sapiens-no activity with diphosphate as phosphate donor, no activity with triphosphate and 2'-deoxyadenosine as substrates660855---
additional information?-Bacillus subtilis-substrate specificity, enzyme is specific for deoxyadenosine and deoxycytidine640561---
additional information?-Dictyostelium discoideumQ54YL2cannot efficiently phosphorylate any other native deoxyribonucleoside than deoxyadenosine675444---
additional information?-Mycoplasma mycoidesQ93IG4less than 1% activity with dGTP, deoxyuridine, deoxythymidine, 1-beta-D-arabinofuranosyl uracil, 2',3'-dideoxyguanosine, 2',3'-dideoxycytidine, cytidine, acyclovir, ganciclovir, 2'-deoxy-2'-fluoro-1-beta-D-arabinofuranosyl-5-iodouracil, and 5-fluoro-2'-deoxyuridine675428---
additional information?-Dictyostelium discoideumQ54YL2enzyme cannot effectively phopshorylate any other native deoxyribonucleodie besides 2'-deoxyadenosine675444---

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
ATP + 2'-deoxyadenosineADP + 2'-deoxyadenosine 5'-phosphate
show the reaction diagram		Homo sapiens--662985, 660855--
ATP + 2'-deoxycytidineADP + 2'-deoxycytidine 5'-phosphate
show the reaction diagram		Homo sapiens--662985, 660855--
ATP + deoxyadenosineADP + dAMP
show the reaction diagram		Dictyostelium discoideumQ54YL2-675444--
ATP + deoxyadenosineADP + 5'-dAMP
show the reaction diagram		Homo sapiens-key anabolic enzyme for activation of purine and pyrimidine deoxyribonucleosides as well as cytosine arabinoside and other anti-tumour drugs641937-641937
ATP + deoxyadenosineADP + 5'-dAMP
show the reaction diagram		Bos taurus-involved in biosynthesis of nucleoside monophosphates from preformed deoxyribonucleosides641977-641977
ATP + deoxyadenosineADP + 5'-dAMP
show the reaction diagram		Lactobacillus acidophilusP0C1F9dGK/dAK plays an essential role in generating the dexyribonucleotide precursors, dGTP and dATP, for DNA metabolism640567-640567
ATP + deoxyadenosineADP + 5'-dAMP
show the reaction diagram		Mus musculus-involved in nucleoside metabolism641984-641984

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
2'-deoxyadenosine-3'-fluoro-3'-triphosphateHomo sapiens-62% of the activity with ATP and deoxycytidine, 1.5% of the activity with ATP and deoxyadenosine662985-
2'-deoxyadenosine-3'-fluoroara-3'-triphosphateHomo sapiens-59% of the activity with ATP and deoxycytidine, 1% of the activity with ATP and deoxyadenosine662985-
2'-deoxyadenosine-3'-triphosphateHomo sapiens-73% of the activity with ATP and deoxycytidine, 6% of the activity with ATP and deoxyadenosine662985-
3'-deoxyadenosine-2'-triphosphateHomo sapiens-97% of the activity with ATP and deoxycytidine, 60% of the activity with ATP and deoxyadenosine662985-
3'-deoxyadenosine-3'-fluoro-2'-triphosphateHomo sapiens-66% of the activity with ATP and deoxycytidine, 4% of the activity with ATP and deoxyadenosine662985-
3'-deoxyadenosine-3'-fluoroxylo-2'-triphosphateHomo sapiens-62% of the activity with ATP and deoxycytidine, 4% of the activity with ATP and deoxyadenosine662985-
ATPHomo sapiens--660855, 662985 2D-image
ATPMycoplasma mycoides--672159, 675428 2D-image
ATPBacillus cereusQ0H0H5-674219 2D-image
ATPDictyostelium discoideumQ54YL2-675444 2D-image
ATPSolanum tuberosum--676722 2D-image
GTPBacillus subtilis--640561 2D-image
additional informationHomo sapiens-triphosphate is active with 2'-deoxycytidine, 40% of the activity with ATP, not with 2'-deoxyadenosine, no activity with diphosphate as phosphate donor660855-

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
Ca2+Bos taurus-activation, can replace Mg2+641981
Mg2+Bacillus subtilis-required for activity640561
Mg2+Lactobacillus acidophilus-required for activity641950
Mg2+Bos taurus-required for activity641974, 641981
Mg2+Rattus norvegicus-required for activity641976
Mg2+Homo sapiens--660855, 662985
Mn2+Bos taurus-activation, can replace Mg2+641981

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
5'-amino-5'-deoxyadenosineHomo sapiens--694483 2D-image
adenine arabinosideLactobacillus acidophilus--641950 2D-image
adenosineLactobacillus acidophilus--641950 2D-image
adenosineRattus norvegicus--641976 2D-image
CHAPSHomo sapiens-at critical micelle concentration641937 2D-image
dADPBos taurus-0.05 mM, 70% inhibition641974 2D-image
dAMPBos taurus-0.2 mM, 36% inhibition641974 2D-image
dATPLactobacillus acidophilus--640565 2D-image
dATPLactobacillus acidophilus-kinetic, mechanism, protects corresponding active site against trypsin inactivation640566 2D-image
dATPLactobacillus acidophilusP0C1F90.5 mM, 90% inhibition of recombinant dAK, end product inhibition640567 2D-image
dATPBos taurus-0.05 mM, 68% inhibition641974 2D-image
dATPMycoplasma mycoidesQ93IG4feedback inhibitor675428 2D-image
dCDPBos taurus-0.001 mM, 60% inhibition641974 2D-image
dCMPBos taurus-0.01 mM, 88% inhibition641974 2D-image
dCTPLactobacillus acidophilus-kinetic, mechanism, protects corresponding active site against trypsin inactivation, enhances inhibition by dATP640566 2D-image
dCTPBos taurus-0.0005 mM, 59% inhibition641974 2D-image
dCTPMycoplasma mycoidesQ93IG4feedback inhibitor675428 2D-image
deoxyadenosineRattus norvegicus-substrate ribavirin641976 2D-image
deoxyguanosineBos taurus-substrate deoxyadenosine641974 2D-image
dGDPBos taurus-0.2 mM, 46% inhibition641974 2D-image
dGMPBos taurus-0.5 mM, 32% inhibition641974 2D-image
dGTPLactobacillus acidophilus-kinetic, mechanism, protects corresponding active site against trypsin inactivation, enhances inhibition by dATP640566 2D-image
dGTPBos taurus-0.2 mM, 64% inhibition641974 2D-image
diphosphateHomo sapiens-competitive inhibition660855 2D-image
p-chloromercuribenzoateRattus norvegicus-0.0007 mM, 50% inhibition of ribavirin and deoxadenosine kinase activity641976 2D-image
ribavirinRattus norvegicus-deoxyadenosine as substrate641976 2D-image
TrypsinLactobacillus acidophilus-substrates protect, end-products, i.e. dATP, dGTP or dCTP, protect corresponding active sites640566-
Hg2+Bos taurus-0.01 mM, 25% inhibition, 0.1 mM, almost complete inhibition641981 2D-image
additional informationBos taurus-not inhibited by adenosine641974-

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
2'-O-CyclocytidineLactobacillus acidophilus-activation641950 2D-image
5-Fluoro-2'-deoxycytidineLactobacillus acidophilus-0.4 mM, 275% activation of deoxyadenosine phosphorylation, half-maximal activation at 0.028 mM641950 2D-image
cytosine arabinosideLactobacillus acidophilus-activation641950 2D-image
DeoxycytidineLactobacillus acidophilus-30 mM, 584% activation of deoxyadenosine phosphorylation, half-maximal activation at 0.1 mM641950 2D-image
deoxycytidine monophosphateLactobacillus acidophilus-10 mM, 409% activation of deoxyadenosine phosphorylation, half-maximal activation at 1.6 mM641950 2D-image
deoxyguanosineLactobacillus acidophilusP0C1F90.1 mM, 5fold activation of recombinant dAK640567 2D-image
deoxyguanosineLactobacillus acidophilus-5 mM, 330% activation of deoxyadenosine phosphorylation, half-maximal activation at 2.2 mM641950 2D-image

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.0335-2',2'-difluorodeoxycytidineBacillus cereusQ0H0H5ratio kcat/Km is 5100 per s and M674219 2D-image
0.192-2',2'-difluorodesoxycytidineStaphylococcus aureusQ0H0H0pH 8.0690459 2D-image
0.0227-2'-deoxyadenosineDictyostelium discoideumQ54YL2-675444 2D-image
0.0292-2'-deoxyadenosineStaphylococcus aureusQ0H0H0pH 8.0690459 2D-image
0.0332-2'-deoxyadenosineBacillus cereusQ0H0H5-674219 2D-image
0.027-2'-deoxycytidineBacillus cereusQ0H0H5-674219 2D-image
0.0344-2'-deoxycytidineStaphylococcus aureusQ0H0H0pH 8.0690459 2D-image
0.146-2'-fluoro-arabinosyl adenineDictyostelium discoideumQ54YL2-675444 2D-image
0.007-ATPHomo sapiens-pH 7.5, 37°C, with deoxycytidine660855 2D-image
0.011-ATPMycoplasma mycoides-pH 7.6, 37°C, recombinant enzyme641988 2D-image
0.016-ATPMycoplasma mycoides-with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2672159 2D-image
0.06-ATPHomo sapiens-pH 7.5, 37°C, with deoxyadenosine660855 2D-image
0.021-CTPMycoplasma mycoides-with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2672159 2D-image
0.022-CTPMycoplasma mycoides-pH 7.6, 37°C, recombinant enzyme641988 2D-image
0.0028-deoxyadenosineLactobacillus acidophilus-pH 8.0, 20°C, recombinant dAK640565 2D-image
0.0049-deoxyadenosineHomo sapiens-pH 7.4, 37°C, CCRF-CEM leukemia cells641987 2D-image
0.0075-deoxyadenosineLactobacillus acidophilus-pH 8.0, 20°C, R79K mutant dAK640565 2D-image
0.01-deoxyadenosineMycoplasma mycoides-with ATP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2672159 2D-image
0.012-deoxyadenosineMycoplasma mycoides-pH 7.6, 37°C, recombinant enzyme641988 2D-image
0.018-deoxyadenosineMycoplasma mycoides-with CTP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2672159 2D-image
0.0227-deoxyadenosineDictyostelium discoideumQ54YL2-675444 2D-image
0.029-deoxyadenosineHomo sapiens-pH 7.4, 37°C, erythrocyte641987 2D-image
0.0332-deoxyadenosineBacillus cereusQ0H0H5in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS674219 2D-image
0.5-deoxyadenosineRattus norvegicus-pH 7.5, 37°C641976 2D-image
5-deoxyadenosineBos taurus-pH 7.8, 37°C641974 2D-image
0.027-DeoxycytidineBacillus cereusQ0H0H5in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS674219 2D-image
0.121-DeoxycytidineMycoplasma mycoides-with ATP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2672159 2D-image
0.146-DeoxycytidineMycoplasma mycoides-with CTP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2672159 2D-image
0.18-DeoxycytidineMycoplasma mycoides-pH 7.6, 37°C, recombinant enzyme641988 2D-image
0.019-deoxyguanosineMycoplasma mycoides-with CTP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2672159 2D-image
0.02-deoxyguanosineMycoplasma mycoides-with ATP as phosphoryl donor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2672159 2D-image
0.021-deoxyguanosineMycoplasma mycoides-pH 7.6, 37°C, recombinant enzyme641988 2D-image
0.146-fludarabineDictyostelium discoideumQ54YL2-675444 2D-image
0.0335-gemcitabineBacillus cereusQ0H0H5in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS674219 2D-image
0.11-MgATP2-Lactobacillus acidophilus-pH 8.0, 20°C, R79K:dGK mutant dAK640565 2D-image
0.15-MgATP2-Lactobacillus acidophilus-pH 8.0, 20°C, R79K mutant dAK640565 2D-image
3.2-ribavirinRattus norvegicus-pH 7.5, 37°C641976 2D-image
0.004-thymidineDictyostelium discoideumQ54YL2; 675444 2D-image
0.074-TriphosphateHomo sapiens-pH 7.5, 37°C, with deoxycytidine660855 2D-image
0.063-TTPMycoplasma mycoides-pH 7.6, 37°C, recombinant enzyme641988 2D-image
0.077-TTPMycoplasma mycoides-with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2672159 2D-image
0.135-UTPMycoplasma mycoides-with deoxyadenosine as phosphoryl acceptor, at room temperature in 0.01 M HEPES (pH 7.4) containing 0.15 M NaCl and 0.002 M MgCl2672159 2D-image
0.18-UTPMycoplasma mycoides-pH 7.6, 37°C, recombinant enzyme641988 2D-image
0.44-MgATP2-Lactobacillus acidophilus-pH 8.0, 20°C, recombinant dAK640565 2D-image
additional information-additional informationHomo sapiens-kinetic study641937-
additional information-additional informationLactobacillus acidophilus-kinetic study641950-
additional information-additional informationHomo sapiens-the reaction with triphosphate follows strict Michaelis-Menten kinetics, while ATP exhibits negative cooperativity660855-

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.19-2',2'-difluorodeoxycytidineStaphylococcus aureusQ0H0H0pH 8.0690459 2D-image
1.82-2'-deoxyadenosineStaphylococcus aureusQ0H0H0pH 8.0690459 2D-image
3.7-2'-deoxyadenosineDictyostelium discoideumQ54YL2-675444 2D-image
0.56-2'-deoxycytidineStaphylococcus aureusQ0H0H0pH 8.0690459 2D-image
3.3-2'-fluoro-arabinosyl adenineDictyostelium discoideumQ54YL2-675444 2D-image
0.84-ATPMycoplasma mycoides-pH 7.6, 37°C, recombinant enzyme641988 2D-image
0.62-CTPMycoplasma mycoides-pH 7.6, 37°C, recombinant enzyme641988 2D-image
1.22-deoxyadenosineBacillus cereusQ0H0H5in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS674219 2D-image
1.48-deoxyadenosineMycoplasma mycoides-pH 7.6, 37°C, recombinant enzyme641988 2D-image
3.7-deoxyadenosineDictyostelium discoideumQ54YL2-675444 2D-image
0.55-DeoxycytidineBacillus cereusQ0H0H5in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS674219 2D-image
1.23-DeoxycytidineMycoplasma mycoides-pH 7.6, 37°C, recombinant enzyme641988 2D-image
0.61-deoxyguanosineMycoplasma mycoides-pH 7.6, 37°C, recombinant enzyme641988 2D-image
3.3-fludarabineDictyostelium discoideumQ54YL2-675444 2D-image
0.17-gemcitabineBacillus cereusQ0H0H5in 50 mM Tris/HCl, pH 8.0, 10 mM dithiothreitol, 2.5 mM ATP, 2.5 mM MgCl2, 3 mg/ml of bovine serum albumin and 0.5 mM CHAPS674219 2D-image
0.0081-thymidineDictyostelium discoideumQ54YL2-675444 2D-image
8.1-thymidineDictyostelium discoideumQ54YL2-675444 2D-image
1.64-TTPMycoplasma mycoides-pH 7.6, 37°C, recombinant enzyme641988 2D-image
0.36-UTPMycoplasma mycoides-pH 7.6, 37°C, recombinant enzyme641988 2D-image

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.035-adenosineRattus norvegicus-pH 7.5, 37°C, substrate deoxyadenosine641976 2D-image
0.057-adenosineRattus norvegicus-pH 7.5, 37°C, substrate ribavirin641976 2D-image
0.001-dATPLactobacillus acidophilus-pH 8.0, 20°C, competitive vs. ATP640565 2D-image
0.0014-dATPLactobacillus acidophilus-pH 8.0, 20°C, noncompetitive vs. deoxadenosine640565 2D-image
0.97-deoxyadenosineRattus norvegicus-pH 7.5, 37°C, substrate ribavirin641976 2D-image
0.08-diphosphateHomo sapiens-pH 7.5, 37°C, with deoxyadenosine, versus ATP660855 2D-image
0.31-diphosphateHomo sapiens-pH 7.5, 37°C, with deoxycytidine, versus ATP or triphosphate660855 2D-image
2.5-ribavirinRattus norvegicus-pH 7.5, 37°C, substrate deoxyadenosine641976 2D-image

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
4.5e-06-Solanum tuberosum-in 30 mM HEPES-NaOH buffer (pH 7.6), 10 mM MgCl2, 1 mM DTT, 3.75 mM ATP and 0.055 mM deoxyadenosine676722
0.007-Homo sapiens-purified recombinant enzyme, substrates deoxycytidine and ATP660855
0.027-Homo sapiens-substrate 2'-deoxycytidine and ATP662985
0.06-Rattus norvegicus-substrate ribavarin641976
0.073-Bos taurus--641981
0.43-Homo sapiens-purified recombinant enzyme, substrates deoxyadenosine and ATP660855
0.54-Homo sapiens-substrate 2'-deoxyadenosine and ATP662985
2.3-Bacillus subtilis-dAdo/dCyd kinase in crude recombinant Escherichia coli cell extract, substrate 2'-deoxyadenosine or 2'-deoxycytidine640561
additional information-Bos taurus-0.00006 mmol/ml min641974
additional information-Mus musculus-activity increases 5.4fold between days 7 and 9 of mouse embryonic development641984

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
7.5-Rattus norvegicus-with deoxyadenosine and ribavirin as substrates641976
7.5-Bos taurus-broad optimum, 10% difference in activity between pH 6.5 and pH 8.5641981
7.5-Homo sapiens-assay at660855
8.5-Bacillus subtilis-assay at640561

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
5.78.8Bos taurus-approx. half-maximal activity at pH 5.7, approx. 80% of maximal activity at pH 8.8641981
69Rattus norvegicus-approx. 30% of maximal activity at pH 6.0 and pH 9.0, respectively641976

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
20-Lactobacillus acidophilus-assay at640566, 641950
37-Bacillus subtilis-assay at640561
37-Bos taurus-assay at641974
37-Rattus norvegicus-assay at641976
37-Bos taurus-assay at641977, 641981
37-Homo sapiens-assay at660855
44-Homo sapiens--641937

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
2750Homo sapiens-approx. half-maximal activity at 27°C and 50°C641937

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
brainHomo sapiens-low activity641099Manually annotated by BRENDA team
CCRF-CEM cellHomo sapiens--641987Manually annotated by BRENDA team
embryoMus musculus--641984Manually annotated by BRENDA team
erythrocyteHomo sapiens-very low activity641987Manually annotated by BRENDA team
granulocyteHomo sapiens-peripheral from adult, very low activity641099Manually annotated by BRENDA team
heartHomo sapiens-low activity641099Manually annotated by BRENDA team
kidneyHomo sapiens-low activity641099Manually annotated by BRENDA team
liverRattus norvegicus--641089, 641976Manually annotated by BRENDA team
liverHomo sapiens--641099Manually annotated by BRENDA team
lungHomo sapiens-low activity641099Manually annotated by BRENDA team
lymphocyteHomo sapiens-peripheral from adult641099Manually annotated by BRENDA team
small intestineHomo sapiens-low activity641099Manually annotated by BRENDA team
spleenHomo sapiens--641099Manually annotated by BRENDA team
spleenHomo sapiens-leukemic641937Manually annotated by BRENDA team
thymocyteHomo sapiens-suspension culture694483Manually annotated by BRENDA team
thymusHomo sapiens-highest activity641099Manually annotated by BRENDA team
thymusBos taurus--641974, 641977, 641981Manually annotated by BRENDA team
tuberSolanum tuberosum--676722Manually annotated by BRENDA team

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
solubleRattus norvegicus---641976Manually annotated by BRENDA team

PDBSCOPCATHORGANISM
2jaq, downloadSCOP (2jaq)CATH (2jaq)Mycoplasma mycoides subsp. mycoides SC

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
26200-Bacillus cereusQ0H0H5SDS-PAGE674219
50000-Mycoplasma mycoides-gel filtration641988
50000-Mycoplasma mycoidesQ93IG4Superdex 75 column gel filtration675428
63000-Bos taurus-gel filtration641981

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
?Dictyostelium discoideumQ54YL2x * 28600, calculated675444
?Staphylococcus aureusQ0H0H0x * 26400, calculated690459
dimerLactobacillus acidophilus-1 * 27200 + 1 * 29000, heterodimers in quarternary structure, SDS-PAGE640566
dimerMycoplasma mycoides-2 * 27000, His-tagged enzyme, SDS-PAGE641988
dimerMycoplasma mycoidesQ93IG42 * 25000, Superdex 75 column gel filtration675428
additional informationLactobacillus acidophilus-both kinase complex I, i.e. dCyd kinase/dAdo kinase I, and kinase complex II, i.e. dGuo kinase/dAdo kinase II, are heterodimers carrying distinct phosphorylation sites for two deoxynucleotide substrates on separate subunits640566
additional informationLactobacillus acidophilusP0C1F9dGK/dAK is composed of 2 nonidentical subunits of approx. 26000 Da640567

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
three-dimensional structural model based on crystal structure of Mycoplasma mycoides deoxyadenosine kinaseDictyostelium discoideumQ54YL2675444
in complex with deoxyadenosine 5'-triphosphate and 2'-deoxycytidine 5'-triphosphate, as well as with products 2'-deoxycytidine 5'-phosphate and 2'-deoxycytidine 5'-diphosphate. Both deoxyadenosine 5'-triphosphate and 2'-deoxycytidine 5'-triphosphate bind to the enzyme in a feedback-inhibitory manner with the deoxyribonucleoside part in the deoxyribonucleoside binding site and the triphosphates in the P-loop. Superposition with human counterparts deoxyguanosine kinase and deoxycytidine kinase. Overall structures are very similar with a few amino acid alterations in the proximity of the active site; sitting drop vapour diffusion method using 24% (w/v) PEG 3350 and 0.2 M KSCNMycoplasma mycoidesQ93IG4675428

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
4-Homo sapiens-half-life of deoxyadenosine kinase activity: 1.5 days641937
37-Homo sapiens-t1/2: 2 h, 1 mM ATP, 0.5 mM dTTP, 1 mM deoxyadenosine or 0.25 3-[(3-cholamidopropyl)dimethylamino]-1-propanesulfonate stabilize641937
42-Rattus norvegicus-first-order rate constant of inactivation: in the absence of dithiothreitol, 14.2/min, in the presence of 10 mM dithiothreitol, 6.82, substrate ribavirin641976
48-Homo sapiens-inactivation of deoxyadenosine kinase activity641937
50-Homo sapiens-80% loss of activity641937
additional information-Homo sapiens-temperature stability of deoxyadenosine kinase activity differs appreciably from deoxycytidine kinase activity641937
additional information-Rattus norvegicus-dithiothreitol protects against heat inactivation641976

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
glycerol, 50%, or dithiothreitol stabilizesBos taurus-641974
stability of deoxyadenosine kinase activity differs appreciably from deoxycytidine kinase activityHomo sapiens-641937
dATP stabilizesLactobacillus acidophilus-640566
dithiothreitol protects against heat inactivation, glycerol and dithiothreitol increase stability during storageRattus norvegicus-641976

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-20°C, 60% glycerol, at least 3 months, slight loss of activityBos taurus-641981
4°C, t1/2: 84 h with and t1/2: 32 h without addition of bovine serum albuminHomo sapiens-641937
ATP, dTTP, deoxyadenosine or CHAPS stabilizes at 4°CHomo sapiens-641937
dATP stabilizes during storageLactobacillus acidophilus-640566

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
glutathione Sepharose FF column chromatography; recombinant proteinBacillus cereusQ0H0H5674219
affinity chromatography on Cibacron Blue F3G-ABos taurus-641977
streptomycin sulfate, protamine sulfate, ammonium sulfate, Sephadex G-150Bos taurus-641974, 641981
affinity chromatographyLactobacillus acidophilus-640566, 641980
recombinant dGK/dAK, dATP-Sepharose affinity chromatographyLactobacillus acidophilusP0C1F9640567
recombinant His-tagged enzyme, metal affinity chromatographyMycoplasma mycoides-641988
Superdex 75 column gel filtrationMycoplasma mycoidesQ93IG4675428
ammonium sulfate, Sephadex G-100, DEAE-cellulose, Sephadex G-75Rattus norvegicus-641976

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
expressed in Escherichia coli; expression in Escherichia coliBacillus cereusQ0H0H5674219
gene yaaF of the yaaG/yaaF operon, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coliBacillus subtilis-640561
expression in Escherichia coliDictyostelium discoideumQ54YL2675444
expression of dGK/dAK in Escherichia coliLactobacillus acidophilusP0C1F9640567
expression in Escherichia coliMycoplasma mycoides-641988
-Staphylococcus aureusQ0H0H0690459

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
D78ALactobacillus acidophilus-mutation on both subunits of dGK/dAK, 0.2% of wild-type dAK activity640565
D78ELactobacillus acidophilus-mutation on both subunits of dGK/dAK, 0.2% of wild-type dAK activity640565
D78NLactobacillus acidophilus-mutation on both subunits of dGK/dAK, 0.2% of wild-type dAK activity640565
D84ALactobacillus acidophilus-mutation in dGK of dAK/dGK, increase of dAK activity641989
D84ELactobacillus acidophilus-mutation in dGK of dAK/dGK, increase of dAK activity641989
D84NLactobacillus acidophilus-mutation in dGK of dAK/dGK, increase of dAK activity641989
R79kLactobacillus acidophilus-2 types of mutants, one bears the mutation on both the dAK and dGK subunits while the other, called R79K:dGK, has the mutation on the dGK subunit only, strong increase in dAK activity, activation of dAK by deoxyguanosine is nearly eliminated in the case of R79K:dGK, while for the tandem mutated R79K mutant a 30% inhibition is observed, inhibition by dATP is reduced640565

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
medicineHomo sapiens-thymocytes lacking adenosine deaminase activity accumulate intracellular dATP and undergo apoptosis. Inhibition of both deoxycytidine kinase and adenosine kinase completely abrogates accumulation of dATP and significantly reduces the induction of apoptosis694483

DISEASETITLE OF PUBLICATIONLINK TO PUBMED
No entries in this field

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISMLINK TO PUBMEDSOURCE
640561Andersen, R.B.; Neuhard, J.Deoxynucleoside kinases encoded by the yaaG and yaaF genes of Bacillus subtilis. Substrate specificity and kinetic analysis of deoxyguanosine kinase with UTP as the preferred phosphate donorJ. Biol. Chem.2765518-55242001Bacillus subtilis PubMed
640565Hong, Y.S.; Ma, G.T.; Ives, D.H.Directed mutagenesis of deoxyguanosine site at arginine 79 up-regulates turnover on deoxyadenosine kinase subunit of heterodimeric enzyme from Lactobacillus acidophilus R26J. Biol. Chem.2706602-66061995Lactobacillus acidophilus PubMed
640566Ikeda, S.; Ma, G.T.; Ives, D.H.Heterodimeric deoxynucleoside kinases of Lactobacillus acidophilus R-26: functional assignment of subunits using limited proteolysis controlled by end-product inhibitorsBiochemistry335328-53341994Lactobacillus acidophilus PubMed
640567Ma, G.T.; Hong, Y.S.; Ives, D.H.Cloning and expression of the heterodimeric deoxyguanosine kinase/deoxyadenosine kinase of Lactobacillus acidophilus R-26J. Biol. Chem.2706595-66011995Lactobacillus acidophilus PubMed
641089Drabikowska, A.K.; Halec, L.; Shugar, D.Purification and properties of adenosine kinase from rat liver: separation from deoxyadenosine kinase activityZ. Naturforsch. C4034-411985Rattus norvegicus-
641099Carson, D.A.; Kaye, J.; Seegmiller, J.E.Lymphospecific toxicity in adenosine deaminase deficiency and purine nucleoside phosphorylase deficiency: possible role of nucleoside kinase(s)Proc. Natl. Acad. Sci. USA745677-56811977Homo sapiens PubMed
641937Kierdaszuk, B.; Eriksson, S.Selective inactivation of the deoxyadenosine phosphorylating activity of pure human deoxycytidine kinase: stabilization of different forms of the enzyme by substrates and biological detergentsBiochemistry294109-41141990Homo sapiens PubMed
641950Deibler, R.D.; Reznik, R.B.; Ives, D.H.Deoxynucleoside kinases from Lactobacilli. Separate interacting sites for deoxycytidine and deoxyadenosineJ. Biol. Chem.2528240-82441977Lactobacillus acidophilus PubMed
641974Krygier, V.; Momparler, R.L.The regulatory properties of deoxyadenosine kinaseBiochim. Biophys. Acta161578-5801968Bos taurus PubMed
641976Streeter, D.G.; Simon, L.N.; Robins, R.K.; Miller, J.P.The phosphorylation of ribavirin by deoxyadenosine kinase from rat liver. Differentiation between adenosine and deoxyadenosine kinaseBiochemistry134543-45491974Rattus norvegicus PubMed
641977Baxter, A.; Carswell, L.M.; Durham, J.P.Purification and detection of deoxyribonucleoside kinases [proceedings]Biochem. Soc. Trans.5979-9811977Bos taurus PubMed
641980Ikeda, S.; Swenson, R.P.; Ives, D.H.Amino-terminal nucleotide-binding sequences of a Lactobacillus deoxynucleoside kinase complex isolated by novel affinity chromatographyBiochemistry278648-86521988Lactobacillus acidophilus PubMed
641981Krygier, V.; Momparler, R.L.Mammalian deoxynucleoside kinases. II. Deoxyadenosine kinase: purification and propertiesJ. Biol. Chem.2462745-27511971Bos taurus PubMed
641984Jenuth, J.P.; Mably, E.R.; Snyder, F.F.Modeling of purine nucleoside metabolism during mouse embryonic development. Relative routes of adenosine, deoxyadenosine, and deoxyguanosine metabolismBiochem. Cell Biol.74219-2251996Mus musculus PubMed
641987Szabados, E.; Duggleby, R.G.; Christopherson, R.I.Metabolism of adenosine and deoxyadenosine by human erythrocytes and CCRF-CEM leukemia cellsInt. J. Biochem. Cell Biol.281405-14141996Homo sapiens PubMed
641988Wang, L.; Westberg, J.; Bolske, G.; Eriksson, S.Novel deoxynucleoside-phosphorylating enzymes in mycoplasmas: evidence for efficient utilization of deoxynucleosidesMol. Microbiol.421065-10732001Acholeplasma laidlawii, Mycoplasma arginini, Mycoplasma mycoides, Mycoplasma pneumoniae PubMed
641989Park, I.; Kim, E.A.; Bang, K.S.; Kim, S.H.; Kim, G.N.; Lee, M.K.; Kil, J.O.Protein engineering of deoxynucleoside kinase from Lactobacillus acidophilus: effect of site-directed mutagenesis on microbial growthJ. Food Sci. Nutr.679-812001Lactobacillus acidophilus-
660855Krawiec, K.; Kierdaszuk, B.; Shugar, D.Inorganic tripolyphosphate (PPP(i)) as a phosphate donor for human deoxyribonucleoside kinasesBiochem. Biophys. Res. Commun.301192-1972003Homo sapiens PubMed
662985Krawiec, K.; Kierdaszuk, B.; Kalinichenko, E.N.; Rubinova, E.B.; Mikhailopulo, I.A.; Eriksson, S.; Munch-Petersen, B.; Shugar, D.Ability of adenosine-2'(3')-deoxy-3'(2')-triphosphates and related analogues to replace ATP as phosphate donor for all human and Drosphila melanogaster deoxyribonucleoside kinasesNucleosides Nucleotides Nucleic Acids221525-15292003Homo sapiens-
672159Pol, E.; Wang, L.Kinetic mechanism of deoxyadenosine kinase from Mycoplasma determined by surface plasmon resonance technologyBiochemistry45513-5222006Mycoplasma mycoides PubMed
674219Sandrini, M.P.; Clausen, A.R.; On, S.L.; Aarestrup, F.M.; Munch-Petersen, B.; Piskur, J.Nucleoside analogues are activated by bacterial deoxyribonucleoside kinases in a species-specific mannerJ. Antimicrob. Chemother.60510-5202007Bacillus cereus PubMed
675428Welin, M.; Wang, L.; Eriksson, S.; Eklund, H.Structure-function analysis of a bacterial deoxyadenosine kinase reveals the basis for substrate specificityJ. Mol. Biol.3661615-16232007Mycoplasma mycoides PubMed
675444Sandrini, M.P.B.; Soederbom, F.; Mikkelsen, N.E.; Piskur, J.Dictyostelium discoideum salvages purine deoxyribonucleosides by highly specific bacterial-like deoxyribonucleoside kinasesJ. Mol. Biol.369653-6642007Dictyostelium discoideum PubMed
676722Katahira, R.; Ashihara, H.Profiles of purine biosynthesis, salvage and degradation in disks of potato (Solanum tuberosum L.) tubersPlanta225115-1262006Solanum tuberosum PubMed
690459Sandrini, M.P.; Shannon, O.; Clausen, A.R.; Bjoerck, L.; Piskur, J.Deoxyribonucleoside kinases activate nucleoside antibiotics in severely pathogenic bacteriaAntimicrob. Agents Chemother.512726-27322007Staphylococcus aureus PubMed
694483Joachims, M.L.; Marble, P.; Knott-Craig, C.; Pastuszko, P.; Blackburn, M.R.; Thompson, L.F.Inhibition of deoxynucleoside kinases in human thymocytes prevents dATP accumulation and induction of apoptosisNucleosides Nucleotides Nucleic Acids27816-8202008Homo sapiens PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 2.7.1.76)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)