Information on EC 2.7.1.4 - fructokinase:
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EC NUMBERCOMMENTARY
2.7.1.4-

RECOMMENDED NAMEGeneOntology No.
fructokinaseGO:0008865

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
ATP + D-fructose = ADP + D-fructose 6-phosphate
show the reaction diagram		----

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
phospho group transfer----

PATHWAYKEGG LinkMetaCyc Link
galactose degradation IV-PWY-6693
heterolactic fermentation-P122-PWY
mannitol cycle-PWY-6531
sucrose degradation I-SUCUTIL-PWY
sucrose degradation III-PWY-621
sucrose degradation IV-PWY-5384
sucrose degradation VI (anaerobic)-PWY-3801

SYSTEMATIC NAMEIUBMB Comments
ATP:D-fructose 6-phosphotransferase-

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
D-fructokinase----
D-fructose(D-mannose)kinase----
FK----
FK1Oryza sativaA2YQL4isoenzyme preferentially expressed under aerobic conditions675666
FK1Solanum tuberosumP37829-676452
FK2Oryza sativaA2YQL4isoenzyme preferentially expressed under anaerobic conditions675666
FRKArabidopsis thaliana-terminology of subtypes641509
FRKBeta vulgarisQ42645terminology of subtypes641509
FRKCamellia japonica, Gleditsia triacanthos, Hordeum vulgare, Lilium longiflorum, Persea americana, Pisum sativum-terminology of subtypes641509
FRKSolanum lycopersicumO04897, Q42896terminology of subtypes; terminology of subtypes641509
FRKSolanum tuberosumP37829terminology of subtypes641509
FRKSpinacia oleracea, Zea mays-terminology of subtypes641509
FRKRattus norvegicus--674062
FRKLeuconostoc pseudomesenteroidesQ8KMM1-674964
Frk1Solanum lycopersicum--676709
Frk2Solanum lycopersicum--676709
Frk3Solanum lycopersicum--676709
Frk4Solanum lycopersicum--676709
fructokinaseClostridium tyrobutyricum--702719
fructokinaseVicia faba--706108
kinase, fructo- (phosphorylating)----
putative fructokinaseLeuconostoc pseudomesenteroidesQ8KMM1-674964
sacK1Lactobacillus plantarum--671484

CAS REGISTRY NUMBERCOMMENTARY
9030-51-7-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Arabidopsis thaliana-641501, 641509--Manually annotated by BRENDA team
Beta vulgarissugar beet641509Q42645SwissProtManually annotated by BRENDA team
Bifidobacterium longuminduction by D-fructose, repression by D-glucose, expressed in Escherichia coli662020--Manually annotated by BRENDA team
Camellia japonicacamellia641509--Manually annotated by BRENDA team
Capsicum annuumpepper641503--Manually annotated by BRENDA team
Catharanthus roseus-641494--Manually annotated by BRENDA team
Clostridium acetobutylicumexpression in Corynebacterium glutamicum661794--Manually annotated by BRENDA team
Clostridium tyrobutyricumstrain ZJU 8235702719--Manually annotated by BRENDA team
Clostridium tyrobutyricum ZJUstrain ZJU 8235702719--Manually annotated by BRENDA team
Escherichia coliK-12641492--Manually annotated by BRENDA team
Gleditsia triacanthoshoney locust641509--Manually annotated by BRENDA team
Glycine max-641486--Manually annotated by BRENDA team
Hordeum vulgare-641509--Manually annotated by BRENDA team
Hordeum vulgareL. cv. Brant, barley641498--Manually annotated by BRENDA team
Klebsiella pneumoniae-641492--Manually annotated by BRENDA team
Lactobacillus plantarumstrain WCFS1671484--Manually annotated by BRENDA team
Lactococcus lactisfructokinase I: induced specifically by growth on sucrose641483--Manually annotated by BRENDA team
Leuconostoc mesenteroides-641481--Manually annotated by BRENDA team
Leuconostoc pseudomesenteroidesstrain ATCC12291674964Q8KMM1TrEMBLManually annotated by BRENDA team
Leuconostoc pseudomesenteroides ATCC12291strain ATCC12291674964Q8KMM1TrEMBLManually annotated by BRENDA team
Lilium longiflorumlily641509--Manually annotated by BRENDA team
Mycobacterium phlei-641478--Manually annotated by BRENDA team
Nicotiana tabacumcultivar Samsun672498--Manually annotated by BRENDA team
Oryza sativa-675666--Manually annotated by BRENDA team
Oryza sativa-675666A2YQL4SwissProtManually annotated by BRENDA team
Oryza sativaisoform OsFKI, expression in Escherichia coli663041Q0JGZ6SwissprotManually annotated by BRENDA team
Oryza sativaisoform OsFKII, expression in Escherichia coli663041Q0J8G4SwissprotManually annotated by BRENDA team
Persea americanaavocado641509--Manually annotated by BRENDA team
Pisum sativum-641509--Manually annotated by BRENDA team
Pisum sativumL. var. Progress641487--Manually annotated by BRENDA team
Pisum sativumpea641477, 641479, 641482--Manually annotated by BRENDA team
Pseudomonas sp.KN-21641507--Manually annotated by BRENDA team
Pseudomonas sp. KN-21KN-21641507--Manually annotated by BRENDA team
Rattus norvegicus-674062--Manually annotated by BRENDA team
Rhizobium leguminosarumbv.trifolii641500--Manually annotated by BRENDA team
Saccharum sp.var N19, isoforms FRK1 and FRK2663153, 663170--Manually annotated by BRENDA team
Schistosoma mansonimale worms641480--Manually annotated by BRENDA team
Solanum lycopersicum-641502, 641505, 641506, 641509, 641511, 676709--Manually annotated by BRENDA team
Solanum lycopersicum-641509O04897, Q42896SwissProtManually annotated by BRENDA team
Solanum lycopersicumisoform FRK3663167Q6VWJ5SwissprotManually annotated by BRENDA team
Solanum lycopersicumMill.641508, 641510--Manually annotated by BRENDA team
Solanum tuberosum-640238, 640239, 641484, 641499--Manually annotated by BRENDA team
Solanum tuberosum-641509P37829SwissProtManually annotated by BRENDA team
Solanum tuberosumcultivars Desiree and Record676452P37829SwissProtManually annotated by BRENDA team
Solanum tuberosumcv. Record641512--Manually annotated by BRENDA team
Spinacia oleraceaspinach641509--Manually annotated by BRENDA team
Streptomyces violaceoruber-641495, 641496, 641497--Manually annotated by BRENDA team
Thermococcus litoralisexpression in Escherichia coli with C-terminal His-tag661678--Manually annotated by BRENDA team
Vibrio alginolyticus-641492--Manually annotated by BRENDA team
Vicia faba-706108--Manually annotated by BRENDA team
Zea mays-641485, 641488, 641509--Manually annotated by BRENDA team
Zea maysisoform FRK1, recombinant protein with His6-tag663163Q6XZ79SwissprotManually annotated by BRENDA team
Zea maysisoform FRK2, recombinant protein with His6-tag663163Q6XZ78SwissprotManually annotated by BRENDA team
Zymomonas mobilis-641057, 641504--Manually annotated by BRENDA team
Zymomonas mobilisexpression in Escherichia coli641493--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
metabolismClostridium tyrobutyricum-sucrose metabolism702719

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Escherichia coli--641492-641492?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Rattus norvegicus--674062--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Hordeum vulgare--641498, 641509--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Spinacia oleracea--641509--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Pisum sativum--641477-641477?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Pisum sativum--641479, 641482--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Pisum sativum--641482-641482?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Pisum sativum--641487, 641509--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Zea mays--641485, 641488, 641509--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Solanum tuberosum--640238, 640239, 641484, 641499, 641512--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Nicotiana tabacum--672498--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Glycine max--641486--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Arabidopsis thaliana--641501, 641509--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Lactococcus lactis--641483-641483?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Pseudomonas sp.--641507--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Mycobacterium phlei--641478--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Leuconostoc mesenteroides--641481--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Klebsiella pneumoniae--641492-641492?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Solanum lycopersicum--641505---
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Solanum lycopersicum--641502, 641506--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Solanum lycopersicumO04897, Q42896-641509--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Solanum lycopersicum--641510, 641511, 676709--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Capsicum annuum--641503--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Schistosoma mansoni--641480-641480?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Streptomyces violaceoruber--641495, 641496--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Zymomonas mobilis--641057, 641493, 641504--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Lilium longiflorum--641509--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Oryza sativaA2YQL4-675666--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Rhizobium leguminosarum--641500--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Persea americana--641509--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Catharanthus roseus--641494--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Vibrio alginolyticus--641492-641492?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Saccharum sp.--663170--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Gleditsia triacanthos--641509--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Beta vulgarisQ42645-641509--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Solanum tuberosumP37829-641509, 676452--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Solanum tuberosum--676452--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Camellia japonica--641509--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Oryza sativaQ0J8G4, Q0JGZ6-663041--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Zea maysQ6XZ78, Q6XZ79-663163--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Solanum lycopersicumQ6VWJ5-663167--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Leuconostoc pseudomesenteroidesQ8KMM1-674964--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Bifidobacterium longum-highly specific for D-fructose662020--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Solanum tuberosum-higher affinity for ATP than for other NTPs640239--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Pisum sativum-specific for fructose641487--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Glycine max-specific for fructose641486-641486?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Streptomyces violaceoruber-specific for fructose641497-641497?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Zymomonas mobilis-specific for fructose641057-641057?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Catharanthus roseus-specific for fructose641494--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Solanum tuberosum-best phosphate donor for isozymes FK-II and FK-III641484--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Zymomonas mobilis-specific for fructose and ATP641057-641057?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Streptomyces violaceoruber-inducible enzyme641496, 641497--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Hordeum vulgare, Spinacia oleracea, Pisum sativum, Zea mays, Arabidopsis thaliana, Lilium longiflorum, Persea americana, Gleditsia triacanthos-fructose metabolism641509--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Beta vulgarisQ42645fructose metabolism641509--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Solanum tuberosumP37829fructose metabolism641509--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Solanum lycopersicumO04897, Q42896fructose metabolism641509--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Camellia japonica-fructose metabolism641509--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Solanum tuberosum-sucrose to starch synthetic pathway641512--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Solanum lycopersicum-sucrose to starch synthetic pathway641511--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Saccharum sp.-highly specific for D-fructose, both isoforms663153--?
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Thermococcus litoralis-highly specific for D-fructose, minor products are D-fructose 2-phosphate and D-fructose-3 phosphate661678--?
ATP + D-glucoseADP + D-glucose 6-phosphate
show the reaction diagram		Pisum sativum--641487---
ATP + D-glucoseADP + D-glucose 6-phosphate
show the reaction diagram		Oryza sativaA2YQL4-675666--?
ATP + D-glucoseADP + D-glucose 6-phosphate
show the reaction diagram		Pisum sativum-low activity641477--?
ATP + D-glucoseADP + D-glucose 6-phosphate
show the reaction diagram		Solanum tuberosum-isoenzyme FK-I and FK-II less than 5% of the activity with fructose, FK-III no activity641484--?
ATP + D-glucoseADP + D-glucose 6-phosphate
show the reaction diagram		Saccharum sp.-isoform FRK1, 1.6% of activity with D-fructose, isoform FRK2, 7.8% of activity with D-fructose663153--?
ATP + D-mannoseADP + D-mannose 6-phosphate
show the reaction diagram		Lactococcus lactis--641483-641483?
ATP + D-mannoseADP + D-mannose 6-phosphate
show the reaction diagram		Pisum sativum-low activity641477--?
ATP + D-mannoseADP + D-mannose 6-phosphate
show the reaction diagram		Leuconostoc mesenteroides-D-fructose and D-mannose phosphorylated at equivalent rates641481-641481?
ATP + D-mannoseADP + D-mannose 6-phosphate
show the reaction diagram		Solanum tuberosum-isoenzyme FK-I and FK-II low activity, less than 5% of D-fructose, FK-III no activity641484--?
ATP + D-mannoseADP + D-mannose 6-phosphate
show the reaction diagram		Saccharum sp.-isoform FRK1, 2.2% of activity with D-fructose, isoform FRK2, 2.4% of activity with D-fructose663153--?
CTP + D-fructoseCDP + D-fructose 6-phosphate
show the reaction diagram		Solanum tuberosum--641484---
CTP + D-fructoseCDP + D-fructose 6-phosphate
show the reaction diagram		Solanum tuberosum--640239--?
CTP + D-fructoseCDP + D-fructose 6-phosphate
show the reaction diagram		Arabidopsis thaliana--641501--?
CTP + D-fructoseCDP + D-fructose 6-phosphate
show the reaction diagram		Zymomonas mobilis-no activity641057---
CTP + D-fructoseCDP + D-fructose 6-phosphate
show the reaction diagram		Zymomonas mobilis-very low activity641493--?
CTP + D-fructoseCDP + D-fructose 6-phosphate
show the reaction diagram		Streptomyces violaceoruber-30% of the activity with ATP641497--?
CTP + D-fructoseCDP + D-fructose 6-phosphate
show the reaction diagram		Pseudomonas sp.-40% of the activity with ATP641507--?
CTP + D-fructoseCDP + D-fructose 6-phosphate
show the reaction diagram		Zea mays-18% of the activity with ATP641485--?
CTP + D-fructoseCDP + D-fructose 6-phosphate
show the reaction diagram		Catharanthus roseus-higher activity than with ATP641494--?
CTP + D-fructoseCDP + D-fructose 6-phosphate
show the reaction diagram		Zea mays-7.4times higher activity than with ATP641488--?
CTP + D-fructoseCDP + D-fructose 6-phosphate
show the reaction diagram		Bifidobacterium longum-6% of activity with ATP662020--?
CTP + D-fructoseCDP + D-fructose 6-phosphate
show the reaction diagram		Thermococcus litoralis-16% of activity with ATP661678--?
CTP + D-fructoseCDP + D-fructose 6-phosphate
show the reaction diagram		Saccharum sp.-isoform FRK1, 35% of activity with ATP, isoform FRK2, 83% of activity with ATP663170--?
D-fructose + ATPD-fructose 6-phosphate + ADP
show the reaction diagram		Vicia faba--706108--?
D-fructose + ATPD-fructose 6-phosphate + ADP
show the reaction diagram		Clostridium tyrobutyricum--702719--?
dATP + D-fructosedADP + D-fructose 6-phosphate
show the reaction diagram		Lactococcus lactis--641483--?
dATP + D-fructosedADP + D-fructose 6-phosphate
show the reaction diagram		Streptomyces violaceoruber-80% of the activity with ATP641497--?
dATP + D-fructosedADP + D-fructose 6-phosphate
show the reaction diagram		Pseudomonas sp.-90% of the activity with ATP641507--?
GTP + D-fructoseGDP + D-fructose 6-phosphate
show the reaction diagram		Zea mays--641485--?
GTP + D-fructoseGDP + D-fructose 6-phosphate
show the reaction diagram		Solanum tuberosum--640239--?
GTP + D-fructoseGDP + D-fructose 6-phosphate
show the reaction diagram		Arabidopsis thaliana--641501--?
GTP + D-fructoseGDP + D-fructose 6-phosphate
show the reaction diagram		Lactococcus lactis--641483--?
GTP + D-fructoseGDP + D-fructose 6-phosphate
show the reaction diagram		Solanum lycopersicum--641505---
GTP + D-fructoseGDP + D-fructose 6-phosphate
show the reaction diagram		Solanum lycopersicum--641506, 641510--?
GTP + D-fructoseGDP + D-fructose 6-phosphate
show the reaction diagram		Pseudomonas sp.-30% of the activity with ATP641507--?
GTP + D-fructoseGDP + D-fructose 6-phosphate
show the reaction diagram		Streptomyces violaceoruber-50% of the activity with ATP641497--?
GTP + D-fructoseGDP + D-fructose 6-phosphate
show the reaction diagram		Solanum tuberosum-best phosphate donor for isozyme FK-I641484--?
GTP + D-fructoseGDP + D-fructose 6-phosphate
show the reaction diagram		Zea mays-3fold higher activity than with ATP641488--?
GTP + D-fructoseGDP + D-fructose 6-phosphate
show the reaction diagram		Bifidobacterium longum-23% of activity with ATP662020--?
GTP + D-fructoseGDP + D-fructose 6-phosphate
show the reaction diagram		Thermococcus litoralis-62% of activity with ATP661678--?
GTP + D-fructoseGDP + D-fructose 6-phosphate
show the reaction diagram		Saccharum sp.-isoform FRK1, 23% of activity with ATP, isoform FRK2, 135% of activity with ATP663170--?
ITP + D-fructoseIDP + D-fructose 6-phosphate
show the reaction diagram		Lactococcus lactis--641483--?
ITP + D-fructoseIDP + D-fructose 6-phosphate
show the reaction diagram		Zymomonas mobilis--641057--?
ITP + D-fructoseIDP + D-fructose 6-phosphate
show the reaction diagram		Streptomyces violaceoruber-60% of the activity with ATP641497--?
ITP + D-fructoseIDP + D-fructose 6-phosphate
show the reaction diagram		Pseudomonas sp.-25% of the activity with ATP641507--?
ITP + D-fructoseIDP + D-fructose 6-phosphate
show the reaction diagram		Thermococcus litoralis-73% of activity with ATP661678--?
TTP + D-fructoseTDP + D-fructose 6-phosphate
show the reaction diagram		Arabidopsis thaliana--641501--?
TTP + D-fructoseTDP + D-fructose 6-phosphate
show the reaction diagram		Bifidobacterium longum-0.18% of activity with ATP662020--?
UTP + D-fructoseUDP + D-fructose 6-phosphate
show the reaction diagram		Hordeum vulgare--641498--?
UTP + D-fructoseUDP + D-fructose 6-phosphate
show the reaction diagram		Solanum tuberosum--641484---
UTP + D-fructoseUDP + D-fructose 6-phosphate
show the reaction diagram		Solanum tuberosum--640239--?
UTP + D-fructoseUDP + D-fructose 6-phosphate
show the reaction diagram		Arabidopsis thaliana--641501--?
UTP + D-fructoseUDP + D-fructose 6-phosphate
show the reaction diagram		Lactococcus lactis--641483--?
UTP + D-fructoseUDP + D-fructose 6-phosphate
show the reaction diagram		Solanum lycopersicum--641505---
UTP + D-fructoseUDP + D-fructose 6-phosphate
show the reaction diagram		Solanum lycopersicum--641506--?
UTP + D-fructoseUDP + D-fructose 6-phosphate
show the reaction diagram		Zymomonas mobilis--641057--?
UTP + D-fructoseUDP + D-fructose 6-phosphate
show the reaction diagram		Zea maysQ6XZ78, Q6XZ79-663163--?
UTP + D-fructoseUDP + D-fructose 6-phosphate
show the reaction diagram		Streptomyces violaceoruber-20% of the activity with ATP641497--?
UTP + D-fructoseUDP + D-fructose 6-phosphate
show the reaction diagram		Zea mays-14% of the activity with ATP641485--?
UTP + D-fructoseUDP + D-fructose 6-phosphate
show the reaction diagram		Pseudomonas sp.-80% of the activity with ATP641507--?
UTP + D-fructoseUDP + D-fructose 6-phosphate
show the reaction diagram		Catharanthus roseus-higher activity than with ATP641494--?
UTP + D-fructoseUDP + D-fructose 6-phosphate
show the reaction diagram		Thermococcus litoralis-16% of activity with ATP661678--?
UTP + D-fructoseUDP + D-fructose 6-phosphate
show the reaction diagram		Saccharum sp.-isoform FRK1, 39% of activity with ATP, isoform FRK2, 84% of activity with ATP663170--?
ITP + D-fructoseIDP + D-fructose 6-phosphate
show the reaction diagram		Bifidobacterium longum-78% of activity with ATP662020--?
additional information?-Pisum sativum--641487---
additional information?-Zymomonas mobilis-no activity with GTP641057---
additional information?-Pisum sativum-no substrate: L-sorbose641477---
additional information?-Schistosoma mansoni-no substrate: L-sorbose641480---
additional information?-Lactococcus lactis-very low activity as phosphate donors: dGTP, TTP, XTP641483---
additional information?-Solanum tuberosum-no substrate: D-mannose640239---
additional information?-Solanum lycopersicum-no substrate: D-mannose641510---
additional information?-Zymomonas mobilis-no substrate: D-mannose641057---
additional information?-Solanum tuberosum-no substrate: D-glucose640238, 640239, 641499---
additional information?-Solanum lycopersicum-no substrate: D-glucose641510---
additional information?-Zymomonas mobilis-no substrate: D-glucose641057---
additional information?-Catharanthus roseus-no substrate: D-glucose641494---
additional information?-Pisum sativum-discussion of possible significance of the enzyme in plant carbohydrate metabolism641479---
additional information?-Thermococcus litoralis-no substrate: ADP, GDP, phosphoenolpyruvate, diphosphate661678---
additional information?-Zea maysQ6XZ78, Q6XZ79no substrate: glucose663163---
additional information?-Solanum lycopersicumQ6VWJ5no substrate: glucose663167---

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Escherichia coli--641492--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Hordeum vulgare--641498--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Pisum sativum--641477, 641479, 641482, 641487--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Zea mays--641485, 641488--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Solanum tuberosum--640238, 640239, 641484, 641499--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Glycine max--641486--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Arabidopsis thaliana--641501--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Lactococcus lactis--641483--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Pseudomonas sp.--641507--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Mycobacterium phlei--641478--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Leuconostoc mesenteroides--641481--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Klebsiella pneumoniae--641492--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Solanum lycopersicum--641502, 641506--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Solanum lycopersicumO04897, Q42896-641509--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Solanum lycopersicum--641510--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Capsicum annuum--641503--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Schistosoma mansoni--641480--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Streptomyces violaceoruber--641495--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Zymomonas mobilis--641057, 641493, 641504--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Rhizobium leguminosarum--641500--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Catharanthus roseus--641494--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Vibrio alginolyticus--641492--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Streptomyces violaceoruber-inducible enzyme641496, 641497--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Hordeum vulgare, Spinacia oleracea, Pisum sativum, Zea mays, Arabidopsis thaliana, Lilium longiflorum, Persea americana, Gleditsia triacanthos-fructose metabolism641509--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Beta vulgarisQ42645fructose metabolism641509--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Solanum tuberosumP37829fructose metabolism641509--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Solanum lycopersicumO04897, Q42896fructose metabolism641509--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Camellia japonica-fructose metabolism641509--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Solanum tuberosum-sucrose to starch synthetic pathway641512--
ATP + D-fructoseADP + D-fructose 6-phosphate
show the reaction diagram		Solanum lycopersicum-sucrose to starch synthetic pathway641511--
additional information?-Pisum sativum-discussion of possible significance of the enzyme in plant carbohydrate metabolism641479--

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
AMPZymomonas mobilis-inhibitory at low and stimulatory at high ATP concentration641057 2D-image
ATPNicotiana tabacum--672498 2D-image
ATPRattus norvegicus--674062 2D-image
ATPLeuconostoc pseudomesenteroidesQ8KMM1-674964 2D-image
ATPOryza sativaA2YQL4; 675666 2D-image
ATPSolanum tuberosumP37829-676452 2D-image
ATPSolanum lycopersicum--676709 2D-image

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
Ca2+Catharanthus roseus-can partially replace Mg2+ or Mn2+ in activation641494
Ca2+Rhizobium leguminosarum-can partially replace Mg2+ or Mn2+ in activation641500
Cd2+Lactococcus lactis-can partially replace Mg2+ in activation641483
Cd2+Rhizobium leguminosarum-can partially replace Mg2+ in activation641500
Co2+Lactococcus lactis-can partially replace Mg2+ in activation641483
Co2+Streptomyces violaceoruber-can partially replace Mg2+ in activation641495, 641497
Co2+Rhizobium leguminosarum-can partially replace Mg2+ in activation641500
Co2+Thermococcus litoralis-43.7% of activity with Mg2+661678
Cs+Solanum tuberosum-no activation641484
Cs+Glycine max-slight stimulation641486
Cs+Pisum sativum-slight stimulation641487
Fe2+Lactococcus lactis-can partially replace Mg2+ in activation641483
K+Pisum sativum-required641479
K+Solanum tuberosum-strong activation of isozyme FK-III, lower activation of FK-I and FK-II641484
K+Glycine max-monovalent cation required for maximal activity, K+ most effective641486
K+Pisum sativum-stimulates641487
K+Hordeum vulgare-stimulates641498
K+Arabidopsis thaliana-activates around 10-20 mM641501
Li+Solanum tuberosum-weak activation of FK-I, no activation of FK-II and FK-III641484
Li+Glycine max-slight stimulation641486
Li+Pisum sativum-slight stimulation641487
Mg2+Solanum tuberosum-maximal activation when total Mg2+ concentration is about 1.5 mM higher than the concentration of ATP640239
Mg2+Pisum sativum-required641479
Mg2+Lactococcus lactis-Km: 0.53 mM; obligate requirement for divalent metal ion, maximal activity with 10 mM Mg2+, other cations can partially replace Mg2+: Co2+, Fe2+, Mn2+, Ni2+, Zn2+, Cd2+641483
Mg2+Glycine max-at 1 mM ATP maximal activity with 1.5 mM MgCl2, higher concentrations inhibit; Km: 0.095 mM for MgATP2-641486
Mg2+Pisum sativum-Km: 0.06 mM for MgATP2-; maximal activity when ATP and MgCl2 are present at approximately equal concentrations, inhibition by excess of either MgCl2 or ATP; required641487
Mg2+Catharanthus roseus-Mg2+ or Mn2+ required641494
Mg2+Streptomyces violaceoruber-required641495
Mg2+Streptomyces violaceoruber-activation by free Mg2+641497
Mg2+Rhizobium leguminosarum-required641500
Mg2+Arabidopsis thaliana--641501
Mg2+Capsicum annuum-required641503
Mg2+Pseudomonas sp.--641507
Mg2+Solanum lycopersicum-required641511
Mg2+Thermococcus litoralis--661678
Mn2+Pisum sativum-can partially replace Mg2+ in activation641479
Mn2+Lactococcus lactis--641483
Mn2+Glycine max-can partially replace Mg2+ in activation; inhibition above 0.5 mM at 1 mM ATP641486
Mn2+Catharanthus roseus-Mn2+ or Mg2+ required641494
Mn2+Rhizobium leguminosarum-can partially replace Mg2+ in activation641500
Mn2+Pseudomonas sp.-can partially replace Mg2+ in activation641507
Na+Pisum sativum-can partially replace K+ in activation641479
Na+Glycine max-slight stimulation641486
Na+Pisum sativum-slight stimulation641487
NH4+Pisum sativum-can partially replace K+ in activation641479
NH4+Glycine max-can partially replace K+ in activation641486
NH4+Pisum sativum-stimulates641487
Ni2+Lactococcus lactis-can partially replace Mg2+ in activation641483
Ni2+Thermococcus litoralis-8.7% of activity with Mg2+661678
Rb+Pisum sativum-can partially replace K+ in activation641479
Rb+Glycine max-can partially replace K+ in activation641486
Rb+Pisum sativum-stimulates641487
Sr2+Thermococcus litoralis-1% of activity with Mg2+661678
Zn2+Lactococcus lactis-can partially replace Mg2+ in activation641483
Mn2+Thermococcus litoralis-65.2% of activity with Mg2+661678
additional informationLactococcus lactis-no activation by Ba2+; no activation by Ca2+; no activation by Cu2+; no activation by Sr2+641483
additional informationStreptomyces violaceoruber-no activation by Ca2+; no activation by Cd2+641495
additional informationStreptomyces violaceoruber-no activation by Cd2+; no activation by Cu2+; no activation by Mn2+641497
additional informationRhizobium leguminosarum-no activation by Ba2+; no activation by Cu2+; no activation by Fe2+; no activation by Zn2+641500
additional informationThermococcus litoralis-not active: Ca2+, Zn2+661678

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
ADPSolanum tuberosum-competitive to ATP640239 2D-image
ADPZymomonas mobilis--641057 2D-image
ADPZea mays--641485 2D-image
ADPCatharanthus roseus--641494 2D-image
ADPArabidopsis thaliana-inhibitory at concentrations higher than 10 mM, complete inactivation above 25 mM641501 2D-image
ADPSolanum lycopersicum-inhibition is dependent on fructose concentration641505, 641506 2D-image
ADPSolanum lycopersicum--641510 2D-image
AMPZymomonas mobilis-inhibitory at low and stimulatory at high ATP concentration641057 2D-image
AMPCatharanthus roseus--641494 2D-image
AMPArabidopsis thaliana-milder effect than ADP641501 2D-image
ATPZymomonas mobilis--641057 2D-image
ATPPisum sativum--641487 2D-image
ATPArabidopsis thaliana-substrate inhibition above 5 mM641501 2D-image
ATPBifidobacterium longum-inhibitory above 12 mM662020 2D-image
ATPOryza sativaQ0J8G4, Q0JGZ650 mM, almost complete inhibition; 50 mM, almost complete inhibition663041 2D-image
beta,gamma-methyleneadenosine 5'-triphosphatePisum sativum--641482 2D-image
Ca2+Capsicum annuum-slightly inhibitory at 0.1 mM641503 2D-image
CTPSolanum lycopersicum-above 1 mM641510 2D-image
D-fructoseSolanum tuberosum-substrate inhibition640239 2D-image
D-fructoseZymomonas mobilis-weak641057 2D-image
D-fructosePisum sativum-excess, inhibition at pH 8.2, no inhibition at pH 6.6641479 2D-image
D-fructosePisum sativum-D-isomer; hyperbolic-noncompetitive with respect to both substrates641482 2D-image
D-fructoseSolanum tuberosum-isoenzyme FK-I: substrate inhibition at 4-8 mM, FK-II: substrate inhibition above 1 mM, FK-III: no substrate inhibition641484 2D-image
D-fructoseZea mays--641485 2D-image
D-fructoseGlycine max-above 0.4 mM at pH 8.2, no substrate inhibition at pH 6.6; substrate inhibition641486 2D-image
D-fructosePisum sativum--641487 2D-image
D-fructoseStreptomyces violaceoruber--641497 2D-image
D-fructoseHordeum vulgare-severe inhibition with fructokinase-Ia and-Ib, slight with fructokinase II; substrate inhibition641498 2D-image
D-fructoseSolanum tuberosum-above 2 mM641499 2D-image
D-fructoseArabidopsis thaliana-substrate inhibition above 5 mM641501 2D-image
D-fructoseSolanum lycopersicum-inhibition of Frk2 but not of Frk1 at high concentrations641502 2D-image
D-fructoseSolanum lycopersicum-above 5 mM641505 2D-image
D-fructoseSolanum lycopersicum-above 0.5 mM641506 2D-image
D-fructoseSolanum lycopersicum-inhibition of FKIII, but not of FKII641510 2D-image
D-fructoseSolanum lycopersicum-at physiological cytosolic concentrations641511 2D-image
D-fructoseOryza sativaQ0J8G4, Q0JGZ650 mM, almost complete inhibition663041 2D-image
D-fructoseSaccharum sp.-isoform FRK2, inhibitory above 0.1 mM, 50% inhibition at 0.23 mM663153 2D-image
D-fructoseZea maysQ6XZ78, Q6XZ79above 0.5 mM; above 1 mM663163 2D-image
D-fructoseSolanum lycopersicumQ6VWJ5-663167 2D-image
D-fructoseSaccharum sp.-isoform FRK2, above 0.1 mM, with substrate ATP663170 2D-image
D-fructose 6-phosphateSolanum tuberosum--640239 2D-image
D-fructose 6-phosphatePisum sativum-25% inhibition at 6 mM641487 2D-image
D-fructose 6-phosphateSolanum lycopersicum-weak, non-competitive641505 2D-image
D-galactoseSchistosoma mansoni-30% inhibition at 10 mM641480 2D-image
D-glucosamineSchistosoma mansoni-65% inhibition at 10 mM641480 2D-image
D-glucoseZymomonas mobilis--641057 2D-image
D-glucoseSchistosoma mansoni-70% inhibition at 10 mM641480 2D-image
D-glucosePisum sativum-no inhibition641487 2D-image
D-glucose 6-phosphateZymomonas mobilis--641057 2D-image
D-glucose 6-phosphatePisum sativum-25% inhibition at 6 mM641487 2D-image
D-mannoseSchistosoma mansoni-59% inhibition at 10 mM641480 2D-image
EDTALactococcus lactis--641483 2D-image
GTPSolanum lycopersicum-above 1 mM641510 2D-image
L-sorbosePisum sativum--641482 2D-image
Mg2+Pisum sativum-free, linear-uncompetitive641482 2D-image
Mg2+Pisum sativum-inhibition by excess of either MgCl2 or ATP641487 2D-image
Mg2+Streptomyces violaceoruber-inhibition above 1.5 mM MgCl2 at 1 mM ATP641495 2D-image
Mg2+Capsicum annuum-slightly inhibitory above 5 mM641503 2D-image
Mg2+Solanum lycopersicum-inhibition of FK1 above 1 mM, only little inhibition of FDIII, no inhibition of FKII641510 2D-image
Mg2+Solanum lycopersicum-inhibitory above 2 mM641511 2D-image
MgADP-Pisum sativum--641479, 641482 2D-image
MgADP-Solanum tuberosum--641484 2D-image
MgADP-Glycine max--641486 2D-image
MgADP-Streptomyces violaceoruber-competitive641497 2D-image
MgATP2-Pisum sativum-inhibition at pH 6.6641479 2D-image
MgATP2-Pisum sativum-linear-uncompetitive with respect to fructose641482 2D-image
MgATP2-Streptomyces violaceoruber--641497 2D-image
UDPCatharanthus roseus--641494 2D-image
UMPCatharanthus roseus--641494 2D-image
Mn2+Glycine max-can partially replace Mg2+ in activation, inhibition above 0.5 mM at 1 mM ATP641486 2D-image
additional informationSolanum lycopersicumQ6VWJ5not inhibitory: D-glucose, and Mg2+ up to 10 mM663167-

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
cysteinePisum sativum-activates641477 2D-image

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.024-ATPZea maysQ6XZ78, Q6XZ79pH 8.5, 30°C663163 2D-image
0.025-ATPSolanum tuberosum-FK 2, pH 8640239 2D-image
0.026-ATPSolanum tuberosum-FK 1, pH 8640239 2D-image
0.05-ATPCatharanthus roseus-with D-fructose as cosubstrate, pH 7.4, 30°C641494 2D-image
0.062-ATPSolanum tuberosum-isoenzyme FK-I, 25°C, pH 8.5641484 2D-image
0.087-ATPSolanum tuberosum-isoenzyme FK-II, 25°C, pH 8.5641484 2D-image
0.11-ATPSolanum lycopersicum-FKI, pH 8, 25°C641506 2D-image
0.13-ATPSolanum lycopersicum-recombinant enzyme, pH 8, 25°C641505 2D-image
0.14-ATPSaccharum sp.-isoform FRK1, pH 8.0, 25°C663170 2D-image
0.15-ATPSolanum lycopersicum-FKII, pH 8, 25°C641506 2D-image
0.16-ATPZymomonas mobilis-; pH 7.4, 30°C641057 2D-image
0.18-ATPSaccharum sp.-isoform FRK2, pH 8.0, 25°C663170 2D-image
0.187-ATPZea maysQ6XZ78, Q6XZ79pH 8.5, 30°C663163 2D-image
0.24-ATPSolanum tuberosum-FK 3, pH 8640239 2D-image
0.279-ATPSolanum tuberosum-isoenzyme FK-III, 25°C, pH 8.5641484 2D-image
0.59-ATPLactococcus lactis--641483 2D-image
0.7-ATPZea mays-30°C, pH 8.5641485 2D-image
0.7-ATPPseudomonas sp.-pH 7.6, 45°C641507 2D-image
0.756-ATPBifidobacterium longum--662020 2D-image
0.81-ATPThermococcus litoralis-pH 7.5, 80°C661678 2D-image
0.156-CTPSolanum tuberosum-isoenzyme FK-I, 25°C, pH 8.5641484 2D-image
0.158-CTPSolanum tuberosum-isoenzyme FK-III, 25°C, pH 8.5641484 2D-image
0.268-CTPSolanum tuberosum-isoenzyme FK-II, 25°C, pH 8.5641484 2D-image
0.006-D-fructoseHordeum vulgare-fructokinase Ib, pH 7.6, 25°C641498 2D-image
0.012-D-fructoseHordeum vulgare-fructokinase II, pH 7.6, 25°C641498 2D-image
0.018-D-fructoseSaccharum sp.-isoform FRK2, pH 8.0663153 2D-image
0.028-D-fructoseSaccharum sp.-isoform FRK1, pH 8.0663153 2D-image
0.028-D-fructoseSaccharum sp.-isoform FRK1, pH 8.0, 25°C663170 2D-image
0.041-D-fructoseSolanum tuberosum-isoenzyme FK-I, 25°C, pH 8.5641484 2D-image
0.054-D-fructoseSolanum lycopersicum-Frk2, pH 7.5, 37°C641502 2D-image
0.055-D-fructoseGlycine max-30°C, pH 6.6641486 2D-image
0.057-D-fructosePisum sativum--641479 2D-image
0.06-D-fructosePisum sativum-30°C, pH 8641487 2D-image
0.064-D-fructoseSolanum tuberosum-FK 1, pH 8640239 2D-image
0.068-D-fructoseBeta vulgarisQ42645-641509 2D-image
0.07-D-fructoseZymomonas mobilis-with ATP as cosubstrate, pH 7.4, 30°C641057 2D-image
0.074-D-fructoseSaccharum sp.-isoform FRK2, pH 8.0, 25°C663170 2D-image
0.075-D-fructoseSolanum lycopersicumQ6VWJ5-663167 2D-image
0.077-D-fructoseGlycine max-30°C, pH 8.2641486 2D-image
0.085-D-fructoseSpinacia oleracea-FK1641509 2D-image
0.09-D-fructoseSolanum tuberosum-FK 2, pH 8640239 2D-image
0.1-D-fructoseSolanum tuberosum-FK 3, pH 8640239 2D-image
0.109-D-fructoseZea maysQ6XZ78, Q6XZ79pH 8.5, 30°C663163 2D-image
0.116-D-fructoseSolanum tuberosum-isoenzyme FK-II, 25°C, pH 8.5641484 2D-image
0.12-D-fructoseHordeum vulgare-fructokinase Ia, pH 7.6, 25°C641498 2D-image
0.128-D-fructoseSolanum tuberosum-isoenzyme FK-III, 25°C, pH 8.5641484 2D-image
0.13-D-fructoseZea mays-30°C, pH 8.5641485 2D-image
0.13-D-fructoseCatharanthus roseus-with UTP as cosubstrate, pH 7.4, 30°C641494 2D-image
0.131-D-fructoseSolanum lycopersicum-FKI, pH 8, 25°C641506 2D-image
0.14-D-fructosePersea americana-FRK1641509 2D-image
0.14-D-fructoseSpinacia oleracea-FK2641509 2D-image
0.159-D-fructoseZea maysQ6XZ78, Q6XZ79pH 8.5, 30°C663163 2D-image
0.18-D-fructoseLilium longiflorum--641509 2D-image
0.18-D-fructoseSolanum lycopersicum-pH 7.5641511 2D-image
0.19-D-fructoseGleditsia triacanthos--641509 2D-image
0.201-D-fructoseSolanum lycopersicum-FKII, pH 8, 25°C641506 2D-image
0.22-D-fructoseSolanum lycopersicum-recombinant enzyme, pH 8, 25°C641505 2D-image
0.3-D-fructoseCamellia japonica--641509 2D-image
0.31-D-fructoseLactococcus lactis-pH 7.5, 22°C641483 2D-image
0.31-D-fructoseRhizobium leguminosarum-pH 8, 25°C641500 2D-image
0.34-D-fructoseOryza sativaQ0J8G4, Q0JGZ6pH 7.5, 37°C663041 2D-image
0.4-D-fructoseLeuconostoc mesenteroides-pH 6.9, 25°C641481 2D-image
0.5-D-fructoseStreptomyces violaceoruber--641497 2D-image
0.54-D-fructoseZymomonas mobilis-with UTP as cosubstrate, pH 7.4, 30°C641057 2D-image
0.739-D-fructoseBifidobacterium longum-pH 6.5, 50°C662020 2D-image
1-D-fructosePisum sativum-Km below 1 mM, pH 7.5, 30°C641477 2D-image
1.14-D-fructoseSolanum tuberosum-after expression in yeast, pH 7.5, 37°C641499 2D-image
1.25-D-fructoseZymomonas mobilis-with ITP as cosubstrate, pH 7.4, 30°C641057 2D-image
1.3-D-fructoseSolanum lycopersicum-Frk1, pH 7.5, 37°C641502 2D-image
2.3-D-fructoseThermococcus litoralis-pH 7.5, 80°C661678 2D-image
3.3-D-fructoseOryza sativaQ0J8G4, Q0JGZ6pH 7.5, 37°C663041 2D-image
0.14-D-glucosePisum sativum-30°C, pH 8641487 2D-image
0.9-D-GTPSolanum lycopersicum-FKII, pH 8, 25°C641506 2D-image
0.4-D-mannoseLeuconostoc mesenteroides-pH 6.9, 25°C641481 2D-image
0.1-fructosePersea americana-FRK2641509 2D-image
0.7-fructosePseudomonas sp.-pH 7.6, 45°C641507 2D-image
0.132-GTPSolanum tuberosum-isoenzyme FK-I, 25°C, pH 8.5641484 2D-image
0.172-GTPSolanum tuberosum-isoenzyme FK-III, 25°C, pH 8.5641484 2D-image
0.251-GTPSolanum tuberosum-isoenzyme FK-II, 25°C, pH 8.5641484 2D-image
0.45-GTPSolanum lycopersicum-recombinant enzyme, pH 8, 25°C641505 2D-image
0.69-GTPSolanum lycopersicum-FKI, pH 8, 25°C641506 2D-image
0.25-ITPZymomonas mobilis-; pH 7.4, 30°C641057 2D-image
0.06-MgATP2-Pisum sativum-30°C, pH 8641487 2D-image
0.1-MgATP2-Pisum sativum--641479 2D-image
0.15-UTPCatharanthus roseus-with D-fructose as cosubstrate, pH 7.4, 30°C641494 2D-image
0.222-UTPZea maysQ6XZ78, Q6XZ79pH 8.5, 30°C663163 2D-image
0.228-UTPSolanum tuberosum-isoenzyme FK-III, 25°C, pH 8.5641484 2D-image
0.243-UTPSolanum tuberosum-isoenzyme FK-I, 25°C, pH 8.5641484 2D-image
0.26-UTPZymomonas mobilis-; pH 7.4, 30°C641057 2D-image
0.321-UTPSolanum tuberosum-isoenzyme FK-II, 25°C, pH 8.5641484 2D-image
0.491-UTPZea maysQ6XZ78, Q6XZ79pH 8.5, 30°C663163 2D-image
1.18-UTPSolanum lycopersicum-FKI, pH 8, 25°C641506 2D-image
1.51-UTPSolanum lycopersicum-FKII, pH 8, 25°C641506 2D-image
1.86-UTPSolanum lycopersicum-recombinant enzyme, pH 8, 25°C641505 2D-image
0.2-MgATP2-Streptomyces violaceoruber--641497 2D-image
additional information-additional informationZymomonas mobilis--641057-
additional information-additional informationSchistosoma mansoni--641480-
additional information-additional informationPisum sativum--641487-

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
494-D-fructoseZea maysQ6XZ78, Q6XZ79pH 8.5, 30°C663163 2D-image
8897-D-fructoseZea maysQ6XZ78, Q6XZ79pH 8.5, 30°C663163 2D-image

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.12-ADPSolanum lycopersicum-at 3 mM fructose, pH 8, 25°C641505 2D-image
0.41-ADPSolanum lycopersicum-at 0.5 mM fructose, pH 8, 25°C641505 2D-image
0.0161-D-fructoseSaccharum sp.-isoform FRK2, pH 8.0, 25°C663170 2D-image
0.16-D-fructoseSaccharum sp.-isoform FRK2, pH 8.0663153 2D-image
1.39-D-fructoseSolanum lycopersicum-FKII, pH 8, 25°C641506 2D-image
1.42-D-fructoseZea mays-FK 1, 30°C, pH 8.5641485 2D-image
1.82-D-fructoseSolanum lycopersicum-FKI, pH 8, 25°C641506 2D-image
2-D-fructoseSolanum lycopersicum-pH 7.5641511 2D-image
2.08-D-fructoseZea mays-FK 2, 30°C, pH 8.5641485 2D-image
3-D-fructoseSolanum lycopersicum-pH 8, 25°C641505 2D-image
5.7-D-fructoseSolanum tuberosum-FK 1, pH 8640239 2D-image
6-D-fructoseSolanum tuberosum-FK 2, pH 8640239 2D-image
21-D-fructoseSolanum tuberosum-FK 3, pH 8640239 2D-image
1.3-D-fructose 6-phosphateSolanum tuberosum-FK 1, pH 8640239 2D-image
0.16-MgATP2-Pisum sativum-pH 8, 30°C641482 2D-image
0.2-MgATP2-Streptomyces violaceoruber--641497 2D-image

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
0.00746-Nicotiana tabacum-at 25°C672498
0.094-Pisum sativum--641487
0.8-Solanum tuberosum-FK 3640238
0.84-Bifidobacterium longum-substrate D-fructose, pH 6.5, 50°C662020
1.8-Bifidobacterium longum-substrate ATP, pH 6.5, 50°C662020
2.7-Pisum sativum--641482
10.7-Solanum tuberosum-FK 1640238
10.8-Rhizobium leguminosarum--641500
11.2-Glycine max--641486
12.7-Solanum tuberosum--641484
18.83-Saccharum sp.-isoform FRK1, pH 8.0, 25°C663170
20.2-Solanum tuberosum-FK 2640238
22.59-Solanum lycopersicum--641505
24.2-Solanum lycopersicum-FKII641506
25-Solanum lycopersicum-FKI641506
40-Streptomyces violaceoruber--641495, 641497
44.05-Saccharum sp.-isoform FRK1, pH 8.0, 25°C663170
51.8-Hordeum vulgare-fructokinase Ia and Ib641498
60.3-Hordeum vulgare-fructokinase II641498
76-Leuconostoc mesenteroides--641481
112-Lactococcus lactis--641483
240-Zymomonas mobilis-S69A mutant641504
250-Zymomonas mobilis-wild-type641504
730-Thermococcus litoralis-substrate D-fructose, pH 7.5, 80°C661678
835-Zea maysQ6XZ78, Q6XZ79pH 8.5, 30°C663163
920-Thermococcus litoralis-substrate ATP, pH 7.5, 80°C661678
additional information-Zea mays--641488
additional information-Catharanthus roseus--641494
additional information-Streptomyces violaceoruber--641497
additional information-Leuconostoc pseudomesenteroidesQ8KMM10.49 units/mg at 30°C and pH 5.0674964

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
6-Bifidobacterium longum--662020
6.58Pisum sativum--641477
6.9-Leuconostoc mesenteroides-D-mannose641481
78Lactococcus lactis--641483
78.5Leuconostoc mesenteroides-D-fructose641481
7.2-Catharanthus roseus--641494
7.4-Zymomonas mobilis--641057
7.58Thermococcus litoralis--661678
7.58.5Mycobacterium phlei--641478
7.59.5Zea mays-broad with maximum at pH 8.5641485
7.6-Pseudomonas sp.--641507
7.88.5Solanum tuberosum--640239
89.5Solanum lycopersicum--641506
8-Pisum sativum--641487
8-Hordeum vulgare--641498
8-Rhizobium leguminosarum--641500
8-Solanum lycopersicum--641505
8-Saccharum sp.-isoforms FRK1 and FRK2663170
8.2-Pisum sativum--641479
8.2-Glycine max--641486
8.5-Solanum tuberosum--641484
8.810Streptomyces violaceoruber-lengthy plateau in the alkaline zone641497

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
58Bifidobacterium longum-marked decrease in activity below and above662020
68.5Catharanthus roseus-pH 6: about 55% of activity maximum, pH 8.5: about 35% of activity maximum641494
69Thermococcus litoralis--661678
6-Saccharum sp.-30% loss of activity, both isoform FRK1 and FRK2663170
6.79.3Solanum tuberosum-pH 6.7: about 50% of activity maximum, pH 9.3: about 60% of activity maximum640239
7.48.4Pisum sativum-more than 90% of activity maximum at pH 7.4 and pH 8.4641487
7.49.3Glycine max-more than 90% of activity maximum at pH 7.4 and pH 9.3641486
7.59Saccharum sp.-both isoform FRK1 and FRK2663170
10-Saccharum sp.-40% loss of activity, both isoform FRK1 and FRK2663170

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
25-Solanum tuberosum-assay at641484
25-Hordeum vulgare-assay at641498
30-Pisum sativum-assay at641482
30-Glycine max-assay at641486
30-Pisum sativum-assay at641487
30-Zea mays-assay at641488
30-Thermococcus litoralis-about 1% of activity at 80°C661678
45-Pseudomonas sp.--641507
50-Bifidobacterium longum--662020
80-Thermococcus litoralis--661678
100-Thermococcus litoralis-11.7% of activity at 80°C661678

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
4.65-Rhizobium leguminosarum-IsoGel agarose641500
4.8-Solanum tuberosum-three polypeptides, one-dimensional isoelectric focusing641484
4.87-Zea maysQ6XZ78, Q6XZ79calculated663163
5.34-Zea maysQ6XZ78, Q6XZ79calculated663163
5.44-Solanum lycopersicumQ6VWJ5calculated663167
5.65.9Lactococcus lactis-polypeptide 1, pre-cast Ampholine PAGplates at 10°C641483
5.666.17Solanum lycopersicum-six closely grouped peptides, two-dimensional isoelectric focusing/SDS-PAGE641505
6.076.55Solanum lycopersicum-4 components, FKII, two-dimensional isoelectric focusing/SDS-PAGE641506
6.426.55Solanum lycopersicum-2 components, FKI, two-dimensional isoelectric focusing /SDS-PAGE641506

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
apical budSolanum lycopersicumQ6VWJ5high level663167Manually annotated by BRENDA team
B islet cellRattus norvegicus--674062Manually annotated by BRENDA team
caryopsisOryza sativaA2YQL4; 675666Manually annotated by BRENDA team
cell cultureCatharanthus roseus--641494Manually annotated by BRENDA team
cotyledonGleditsia triacanthos--641509Manually annotated by BRENDA team
fruitSolanum lycopersicum--641505, 641506, 641510, 641511Manually annotated by BRENDA team
fruitSolanum lycopersicum-during early seed development641508Manually annotated by BRENDA team
ileumRattus norvegicus--674062Manually annotated by BRENDA team
internodeSaccharum sp.--663153, 663170Manually annotated by BRENDA team
kernelZea mays--641485, 641488Manually annotated by BRENDA team
leafSolanum tuberosum-high activity: isoenzyme FK3, very low activity: FK1, FK2640238Manually annotated by BRENDA team
leafSpinacia oleracea--641509Manually annotated by BRENDA team
leafSolanum lycopersicumQ6VWJ5high level663167Manually annotated by BRENDA team
leafNicotiana tabacum--672498Manually annotated by BRENDA team
leafSolanum tuberosumP37829-676452Manually annotated by BRENDA team
leafSolanum lycopersicum--676709Manually annotated by BRENDA team
liverRattus norvegicus--674062Manually annotated by BRENDA team
myceliumStreptomyces violaceoruber--641497Manually annotated by BRENDA team
noduleGlycine max--641486Manually annotated by BRENDA team
pancreasRattus norvegicus--674062Manually annotated by BRENDA team
pancreatic isletRattus norvegicus--674062Manually annotated by BRENDA team
parotid glandRattus norvegicus--674062Manually annotated by BRENDA team
pericarpSolanum lycopersicum--641502, 641510, 641511Manually annotated by BRENDA team
pollenCapsicum annuum--641503Manually annotated by BRENDA team
pollenCamellia japonica, Lilium longiflorum--641509Manually annotated by BRENDA team
rootArabidopsis thaliana-FRK1641501Manually annotated by BRENDA team
rootArabidopsis thaliana--641509Manually annotated by BRENDA team
rootBeta vulgarisQ42645-641509Manually annotated by BRENDA team
rootVicia faba-meristem cell706108Manually annotated by BRENDA team
seedPisum sativum-green641477Manually annotated by BRENDA team
seedPisum sativum--641479, 641482, 641487Manually annotated by BRENDA team
seedOryza sativaQ0J8G4, Q0JGZ6immature; immature663041Manually annotated by BRENDA team
seedlingOryza sativaA2YQL4; 675666Manually annotated by BRENDA team
shootArabidopsis thaliana-FRK2641501Manually annotated by BRENDA team
tuberSolanum tuberosum-high activity: isoenzyme FK1, FK2, very low activity: FK3640238Manually annotated by BRENDA team
tuberSolanum tuberosum--640239, 641484, 641512, 676452Manually annotated by BRENDA team
mesocarpPersea americana--641509Manually annotated by BRENDA team
additional informationSolanum lycopersicumQ6VWJ5expressed in all organs examined663167Manually annotated by BRENDA team

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
chloroplastNicotiana tabacum--9507672498Manually annotated by BRENDA team
chloroplast stromaNicotiana tabacum--9570672498Manually annotated by BRENDA team
cytoplasmGlycine max--5737641486Manually annotated by BRENDA team
cytosolSolanum lycopersicum--5829676709Manually annotated by BRENDA team
plastidSolanum lycopersicum--9536676709Manually annotated by BRENDA team
solublePisum sativum---641477Manually annotated by BRENDA team
solubleHordeum vulgare---641498Manually annotated by BRENDA team
solubleCapsicum annuum---641503Manually annotated by BRENDA team
solubleZea maysQ6XZ78, Q6XZ79; -663163Manually annotated by BRENDA team

PDBSCOPCATHORGANISM
1xc3, downloadSCOP (1xc3)CATH (1xc3)Bacillus subtilis (strain 168)
3lm9, downloadSCOP (3lm9)CATH (3lm9)Bacillus subtilis (strain 168)
3ohr, downloadSCOP (3ohr)CATH (3ohr)Bacillus subtilis (strain 168)
3pl2, downloadSCOP (3pl2)CATH (3pl2)Corynebacterium glutamicum (strain ATCC 13032 / DSM 20300 / JCM 1318 / LMG 3730 / NCIMB 10025)
3hj6, downloadSCOP (3hj6)CATH (3hj6)Halothermothrix orenii (strain H 168 / OCM 544 / DSM 9562)
3ubo, downloadSCOP (3ubo)CATH (3ubo)Rhizobium meliloti (strain 1021)
1tyy, downloadSCOP (1tyy)CATH (1tyy)Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
1tz3, downloadSCOP (1tz3)CATH (1tz3)Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
1tz6, downloadSCOP (1tz6)CATH (1tz6)Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
3c8u, downloadSCOP (3c8u)CATH (3c8u)Silicibacter sp. (strain TM1040)
2v78, downloadSCOP (2v78)CATH (2v78)Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
2var, downloadSCOP (2var)CATH (2var)Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
3ljs, downloadSCOP (3ljs)CATH (3ljs)Xylella fastidiosa (strain Temecula1 / ATCC 700964)
3lki, downloadSCOP (3lki)CATH (3lki)Xylella fastidiosa (strain Temecula1 / ATCC 700964)

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
2000080000Streptomyces violaceoruber-glycerol gradient equilibrium sedimentation in the presence of MgATP2-641496
20000-Streptomyces violaceoruber-glycerol gradient equilibrium sedimentation in presence of 10 mM fructose or 10 mM MgCl2641495
34000-Klebsiella pneumoniae-SDS-PAGE641492
36500-Rhizobium leguminosarum-SDS-PAGE641500
37000-Hordeum vulgare-fructokinase-Ia, and fructokinase-1b, gel filtration641498
39000-Escherichia coli-SDS-PAGE641492
44000-Lactococcus lactis-gel filtration641483
47000-Leuconostoc mesenteroides-sucrose density gradient centrifugation641481
54300-Mycobacterium phlei--641478
58000-Thermococcus litoralis-gel filtration661678
59000-Zea mays-gel filtration641485
60000-Lactococcus lactis-nondenaturing equilibrium ultracentrifugation641483
69000-Saccharum sp.-gel filtration, both isoform FRK1 and FRK2663170
70000-Solanum tuberosum-gel filtration641484
70000-Beta vulgarisQ42645native enzyme641509
72000-Pisum sativum-gel filtration641482
72400-Solanum lycopersicum-gel filtration641505
73000-Hordeum vulgare-fructokinase II, gel filtration641498
73000-Solanum lycopersicum-gel filtration641506
75000-Pseudomonas sp.-gel filtration641507
80000-Streptomyces violaceoruber-glycerol gradient equilibrium sedimentation in presence of 10 mM MgATP2-641495
84000-Glycine max-gel filtration641486
85000-Persea americana-native enzyme641509
102000-Solanum tuberosum-FK 1, gel filtration640238
105000-Solanum tuberosum-FK 1, gel filtration640238
118000-Solanum tuberosum-FK 1, gel filtration640238
additional information-Escherichia coli, Klebsiella pneumoniae, Vibrio alginolyticus-nucleotide sequence641492
additional information-Zymomonas mobilis-nucleotide sequence641493

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
?Bifidobacterium longum-x * 32500, calculated, x * 35000, SDS-PAGE662020
?Saccharum sp.-x * 33000, SDS-PAGE, isoforms FRK1 and FRK2663153
?Zea maysQ6XZ78, Q6XZ79x * 34700, SDS-PAGE; x * 35500, SDS-PAGE663163
?Solanum lycopersicumQ6VWJ5x * 41440, calculated663167
dimerLactococcus lactis-2 * 32000, SDS-PAGE after complete reduction with 2-mercaptoethanol641483
dimerSolanum tuberosum-2 * 36000, SDS-PAGE641484
dimerSolanum lycopersicum-2 * 35000, SDS-PAGE641505, 641506
dimerPseudomonas sp.-2 * 37000, SDS-PAGE641507
dimerBeta vulgarisQ426452 * 35400, SDS-PAGE641509
dimerSolanum lycopersicumO04897, Q42896-641509
dimerThermococcus litoralis-2 * 35000, SDS-PAGE, 2 * 36221, calculated for His-tagged protein661678
dimerSaccharum sp.-2 * 33000, SDS-PAGE, both isoform FRK1 and FRK2663170

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
hanging drop vapor diffusion methodZymomonas mobilis-641504

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
6-Streptomyces violaceoruber-1 h, 50% loss of activity641497
6.58Pisum sativum-maximal stability641477
6.58.5Pseudomonas sp.-stable641507

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
640Bifidobacterium longum-gradual decrease of activtiy in this range662020
3739Bifidobacterium longum-15 min, 70% residual activity662020
60-Bifidobacterium longum-15 min, complete loss of activity662020
80-Thermococcus litoralis-4 h, stable661678

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
no significant loss of activity after lyophilization and reconstitution of the enzymeLactococcus lactis-641483
stable after repeated freezing, at -20°C, and thawingLactococcus lactis-641483
freezing and thawing after elution from the first DEAE-cellulose column results in 30% loss of activityPisum sativum-641487
metal binding agents protectPisum sativum-641477
increased heat lability in presence of fructoseStreptomyces violaceoruber-641495
DTT, 10 mM, necessary to stabilize activityZea mays-641488

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
0°C, 0.2 (NH4)2SO4, pH 7.0, stable for several monthsLeuconostoc mesenteroides-641481
-18°C, stable for at least 2 monthsPisum sativum-641487
4°C, 50% loss of activity after 24 hPisum sativum-641487
5°C, stable for 6 weeks at pH 8Pseudomonas sp.-641507
-20°C, in presence of Mg2+ and dithiothreitol, 25% loss of activity after 6 monthsStreptomyces violaceoruber-641497
2°C, pH 7, in presence of Mg2+ and dithioerythritol, 25% loss of activity after 2 weeksStreptomyces violaceoruber-641497
-80°C, PBS buffer, stable for at least 3 monthsThermococcus litoralis-661678

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
recombinant proteinBifidobacterium longum-662020
partialCatharanthus roseus-641494
partial, 3 forms: fructokinase-Ia,-Ib,-IIHordeum vulgare-641498
-Lactococcus lactis-641483
-Leuconostoc mesenteroides-641481
-Mycobacterium phlei-641478
Sephadex G25 column chromatography; Sephadex G25 column chromatographyOryza sativaA2YQL4675666
-Pisum sativum-641477
homogeneityPisum sativum-641482
isoenzyme fructokinase IIIPisum sativum-641487
-Pseudomonas sp.-641507
-Rhizobium leguminosarum-641500
isoforms FRK1 and FRK2Saccharum sp.-663170
-Schistosoma mansoni-641480
-Solanum lycopersicum-641505
400fold, FKI and FKIISolanum lycopersicum-641506
partialSolanum lycopersicum-641511
3 forms: FK-I, FK-II and FK-IIISolanum tuberosum-641484
FK1, FK2, FK3Solanum tuberosum-640238, 640239
400foldStreptomyces violaceoruber-641497
partialStreptomyces violaceoruber-641495
isoenzyme FK-I, FK-IIZea mays-641485
purification of UTP-dependent enzyme: CTP, GTP and UTP are more effective as phosphate donors than ATP, organism also has a nonspecific fructokinase and a third fairly specific activity for ATPZea mays-641488
2-step purification leads to highly purified enzymeZymomonas mobilis-641504

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-Escherichia coli, Klebsiella pneumoniae-641492
expressed in Escherichia coli strain XL1-BlueLeuconostoc pseudomesenteroidesQ8KMM1674964
-Rhizobium leguminosarum-641500
-Solanum lycopersicum-641502, 641505, 641508
-Solanum tuberosum-641499
-Thermococcus litoralis-661678
-Vibrio alginolyticus-641492
-Zymomonas mobilis-641504
expression of Zymomonas mobilis gene in Escherichia coliZymomonas mobilis-641493

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
S69AZymomonas mobilis-more thermostable than wild-type641504
additional informationLeuconostoc pseudomesenteroidesQ8KMM1mutant BPT143 shows highly reduced fructokinase activity674964

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
synthesisClostridium acetobutylicum-enzyme expression strategy for enhancing amino acid production661794

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISMLINK TO PUBMEDSOURCE
640238Renz, A.; Merlo, L.; Stitt, M.Partial purification from potato tubers of three fructokinases and three hexokinases which show differing organ and developmental specificityPlanta190156-1651993Solanum tuberosum-
640239Renz, A.; Stitt, M.Substrate specificity and product inhibition of different forms of fructokinases and hexokinases in developing potato tubersPlanta190166-1751993Solanum tuberosum-
641057Doelle, H.W.Kinetic characteristics and regulatory mechanisms of glucokinase and fructokinase from Zymomonas mobilisEur. J. Appl. Microbiol. Biotechnol.14241-2461982Zymomonas mobilis-
641477Medina, A.; Sols, A.A specific fructokinase in peaBiochim. Biophys. Acta19378-3791956Pisum sativum PubMed
641478Pastuszak, I.; Szymona, M.Isolation of a specific ATP:D-fructose 6-phosphotransferase from Mycobacterium phleiAnn. Univ. Mariae Curie-Sklodowska Sect. D361-111981Mycobacterium phlei PubMed
641479Turner, J.F.; Harrison, D.D.; Copeland, L.Fructokinase (fraction IV) of pea seedsPlant Physiol.60666-6691977Pisum sativum PubMed
641480Bueding, E.; MacKinnon, J.A.Hexokinases of Schistosoma mansoniiJ. Biol. Chem.215495-5061955Schistosoma mansoni PubMed
641481Anderson, R.L.; Sapico, V.L.D-fructose (D-mannose) kinaseMethods Enzymol.42C39-431975Leuconostoc mesenteroides-
641482Copeland, L.; Stone, S.R.; Turner, J.F.Kinetic studies of fructokinase I of pea seedsArch. Biochem. Biophys.233748-7601984Pisum sativum PubMed
641483Thompson, J.; Sackett, D.L.; Donkersloot, J.A.Purification and properties of fructokinase I from Lactococcus lactis. Localization of scrK on the sucrose-nisin transposon Tn5306J. Biol. Chem.26622626-226331991Lactococcus lactis PubMed
641484Gardner, A.; Davies, H.V.; Burch, L.R.Purification and properties of fructokinase from developing tubers of potato (Solanum tuberosum L.)Plant Physiol.100178-1831992Solanum tuberosum PubMed
641485Doehlert, D.C.Separation and characterization of four hexose kinases from developing maize kernelsPlant Physiol.891042-10481989Zea mays PubMed
641486Copeland, L.; Morell, M.Hexose kinases from the plant cytosolic fraction of soybean nodulesPlant Physiol.79114-1171985Glycine max PubMed
641487Copeland, L.; Harrison, D.D.; Turner, J.F.Fructokinase (fraction III) of pea seedsPlant Physiol.62291-2941978Pisum sativum PubMed
641488Doehlert, D.C.Fructokinases from developing maize kernels differ in their specificity for nucleoside triphosphatesPlant Physiol.93353-3551990Zea mays PubMed
641492Aulkemeyer, P.; Ebner, R.M.; Hellenmann, G.; Jahreis, K.; Schmid, K.; Wrieden, S.; Lengeler, J.W.Molecular analysis of two fructokinases involved in sucrose metabolism of enteric bacteriaMol. Microbiol.52913-29221991Escherichia coli, Klebsiella pneumoniae, Vibrio alginolyticus PubMed
641493Zembrzuski, B.; Chilco, P.; Liu, X.L.; Liu, J.; Conway, T.; Scopes, R.Cloning, sequencing, and expression of the Zymomonas mobilis fructokinase gene and structural comparison of the enzyme with other hexose kinasesJ. Bacteriol.1743455-34601992Zymomonas mobilis PubMed
641494Yamashita, Y.; Ashihara, H.Characterization of hexokinase and fructokinase from suspension-cultured Catharanthus roseus cellsZ. Naturforsch.C43827-8341988Catharanthus roseus-
641495Sabater, B.; Delafuente, G.Kinetic properties and related changes of molecular weight in a fructokinase from Streptomyces violaceoruberBiochim. Biophys. Acta377258-2701975Streptomyces violaceoruber PubMed
641496Martin, M.; Sabater, B.Cell-free synthesis of catalytically active fructokinase directed by RNA from Streptomyces violaceoruber grown with fructoseJ. Gen. Microbiol.126203-2101981Streptomyces violaceoruber-
641497Sabater, B.; Sebastian, J.; Asensio, C.Identification and properties of an inducible and highly specific fructokinase from Streptomyces violaceoruberBiochim. Biophys. Acta284414-4201972Streptomyces violaceoruber PubMed
641498Baysdorfer, C.; Kremer, D.F.; Sicher, R.C.Partial purification and characterization of fructokinase activity from barley leavesJ. Plant Physiol.134156-1611989Hordeum vulgare-
641499Dai, N.; Schaffer, A.; Petreikov, M.; Granot, D.Potato (Solanum tuberosum L.) fructokinase expressed in yeast exhibits inhibition by fructose of both in vitro enzyme activity and rate of cell proliferationPlant Sci.128191-1971997Solanum tuberosum-
641500Fennington, G.J., Jr.; Hughes, T.A.The fructokinase from Rhizobium leguminosarum biovar trifolii belongs to group I fructokinase enzymes and is encoded separately from other carbohydrate metabolism enzymesMicrobiology142321-3301996Rhizobium leguminosarum PubMed
641501Gonzali, S.; Pistelli, L.; De Bellis, L.; Alpi, A.Characterization of two Arabidopsis thaliana fructokinasesPlant Sci.1601107-11142001Arabidopsis thaliana PubMed
641502Kanayama, Y.; Granot, D.; Dai, N.; Petreikov, M.; Schaffer, A.; Powell, A.; Bennett, A.B.Tomato fructokinases exhibit differential expression and substrate regulationPlant Physiol.11785-901998Solanum lycopersicum PubMed
641503Karni, L.; Aloni, B.Fructokinase and hexokinase from pollen grains of bell pepper (Capsicum annuum L.): possible role in pollen germination under conditions of high temperature and CO2 enrichmentAnn. Bot.90607-6122002Capsicum annuum PubMed
641504King, K.; Phan, P.; Rellos, P.; Scopes, R.K.Overexpression, purification, and generation of a thermostable variant of Zymomonas mobilis fructokinaseProtein Expr. Purif.7373-3761996Zymomonas mobilis PubMed
641505Martinez-Barajas, E.; Krohn, B.M.; Stark, D.M.; Randall, D.D.Purification and characterization of recombinant tomato fruit (Lycopersicon esculentum Mill.) fructokinase expressed in Escherichia coliProtein Expr. Purif.1141-461997Solanum lycopersicum PubMed
641506Martinez-Barajas, E.; Randall, D.D.Purification and characterization of fructokinase from developing tomato (Lycopersicon esculentum) fruitsPlanta199451-4581996Solanum lycopersicum-
641507Murao, S.; Nakatani, A.; Kaneda, N.Isolation and characterization of fructokinase from Pseudomonas sp. KN-21Biosci. Biotechnol. Biochem.591798-18001995Pseudomonas sp.-
641508Odanaka, S.; Bennett, A.B.; Kanayama, Y.Distinct physiological roles of fructokinase isozymes revealed by gene-specific suppression of Frk1 and Frk2 expression in tomatoPlant Physiol.1291119-11262002Solanum lycopersicum PubMed
641509Pego, J.V.; Smeekens, S.C.Plant fructokinases: a sweet family get-togetherTrends Plant Sci.5531-5362000Arabidopsis thaliana, Beta vulgaris, Camellia japonica, Gleditsia triacanthos, Hordeum vulgare, Lilium longiflorum, Persea americana, Pisum sativum, Solanum lycopersicum, Solanum tuberosum, Spinacia oleracea, Zea mays PubMed
641510Petreikov, M.; Dai, N.; Granot, D.; Schaffer, A.A.Characterization of native and yeast-expressed tomato fruit fructokinase enzymesPhytochemistry58841-8472001Solanum lycopersicum PubMed
641511Schaffer, A.A.; Petreikov, M.Inhibition of fructokinase and sucrose synthase by cytosolic levels of fructose in young tomato fruit undergoing transient starch synthesisPhysiol. Plant.101800-8061997Solanum lycopersicum-
641512Viola, R.Hexose metabolism in disks excised from developing potato (Solanum tuberosum L.) tubers. II. Estimations of fluxes in vivo and evidence that fructokinase catalyzes a near rate-limiting reactionPlanta198186-1961996Solanum tuberosum-
661678Qu, Q.; Lee, S.J.; Boos, W.Molecular and biochemical characterization of a fructose-6-phosphate-forming and ATP-dependent fructokinase of the hyperthermophilic archaeon Thermococcus litoralisExtremophiles8301-3082004Thermococcus litoralis PubMed
661794Moon, M.W.; Kim, H.J.; Oh, T.K.; Shin, C.S.; Lee, J.S.; Kim, S.J.; Lee, J.K.Analyses of enzyme II gene mutants for sugar transport and heterologous expression of fructokinase gene in Corynebacterium glutamicum ATCC 13032FEMS Microbiol. Lett.244259-2662005Clostridium acetobutylicum PubMed
662020Caescu, C.I.; Vidal, O.; Krzewinski, F.; Artenie, V.; Bouquelet, S.Bifidobacterium longum requires a fructokinase (Frk; ATP:D-fructose 6-phosphotransferase, EC 2.7.1.4) for fructose catabolismJ. Bacteriol.1866515-65252004Bifidobacterium longum PubMed
663041Jiang, H.; Dian, W.; Liu, F.; Wu, P.Isolation and characterization of two fructokinase cDNA clones from ricePhytochemistry6247-522003Oryza sativa PubMed
663153Hoepfner, S.W.; Botha, F.C.Expression of fructokinase isoforms in the sugarcane culmPlant Physiol. Biochem.41741-7472003Saccharum sp.-
663163Zhang, S.; Nichols, S.E.; Dong, J.G.Cloning and characterization of two fructokinases from maizePlant Sci.1651051-10582003Zea mays-
663167German, M.A.; Asher, I.; Petreikov, M.; Dai, N.; Schaffer, A.A.; Granot, D.Cloning, expression and characterization of LeFRK3, the fourth tomato (Lycopersicon esculentum Mill.) gene encoding fructokinasePlant Sci.166285-2912004Solanum lycopersicum-
663170Hoepfner, S.W.; Botha, F.C.Purification and characterization of fructokinase from the culm of sugarcanePlant Sci.167645-6542004Saccharum sp.-
671484Saulnier, D.M.; Molenaar, D.; de Vos, W.M.; Gibson, G.R.; Kolida, S.Identification of prebiotic fructooligosaccharide metabolism in Lactobacillus plantarum WCFS1 through microarraysAppl. Environ. Microbiol.731753-17652007Lactobacillus plantarum PubMed
672498Sindelarova, M.; Sindelar, L.; Wilhelmova, N.; Prochazkova, D.Changes in key enzymes of viral-RNA biosynthesis in chloroplasts from PVY and TMV infected tobacco plantsBiol. Plant.49471-4742005Nicotiana tabacum-
674062Giroix, M.H.; Jijakli, H.; Courtois, P.; Zhang, Y.; Sener, A.; Malaisse, W.J.Fructokinase activity in rat liver, ileum, parotid gland, pancreas, pancreatic islet, B and non-B islet cell homogenatesInt. J. Mol. Med.17517-5222006Rattus norvegicus PubMed
674964Helanto, M.; Aarnikunnas, J.; von Weymarn, N.; Airaksinen, U.; Palva, A.; Leisola, M.Improved mannitol production by a random mutant of Leuconostoc pseudomesenteroidesJ. Biotechnol.116283-2942005Leuconostoc pseudomesenteroides PubMed
675666Guglielminetti, L.; Morita, A.; Yamaguchi, J.; Loreti, E.; Perata, P.; Alpi, A.Differential expression of two fructokinases in Oryza sativa seedlings grown under aerobic and anaerobic conditionsJ. Plant Res.119351-3562006Oryza sativa PubMed
676452Davies, H.V.; Shepherd, L.V.; Burrell, M.M.; Carrari, F.; Urbanczyk-Wochniak, E.; Leisse, A.; Hancock, R.D.; Taylor, M.; Viola, R.; Ross, H.; McRae, D.; Willmitzer, L.; Fernie, A.R.Modulation of fructokinase activity of potato (Solanum tuberosum) results in substantial shifts in tuber metabolismPlant Cell Physiol.461103-11152005Solanum tuberosum PubMed
676709Damari-Weissler, H.; Kandel-Kfir, M.; Gidoni, D.; Mett, A.; Belausov, E.; Granot, D.Evidence for intracellular spatial separation of hexokinases and fructokinases in tomato plantsPlanta2241495-15022006Solanum lycopersicum PubMed
702719Jiang, L.; Cai, J.; Wang, J.; Liang, S.; Xu, Z.; Yang, S.T.Phosphoenolpyruvate-dependent phosphorylation of sucrose by Clostridium tyrobutyricum ZJU 8235: evidence for the phosphotransferase transport systemBiores. Technol.101304-3092010Clostridium tyrobutyricum PubMed
706108Polit, J.T.; Ciereszko, I.In situ activities of hexokinase and fructokinase in relation to phosphorylation status of root meristem cells of Vicia faba during reactivation from sugar starvationPhysiol. Plant.135342-3502009Vicia faba PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 2.7.1.4)
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NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
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Protein Mutant Database
InterPro (database of protein families, domains and functional sites)