Information on EC 2.6.1.73 - methionine-glyoxylate transaminase:
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The lowest common taxonomy group for this enzyme is: Brassica

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EC NUMBERCOMMENTARY
2.6.1.73-

RECOMMENDED NAMEGeneOntology No.
methionine-glyoxylate transaminaseGO:0050094

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
L-methionine + glyoxylate = 4-methylthio-2-oxobutanoate + glycine
show the reaction diagram		L-glutamate can also act as donor---

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
amino group transfer----

PATHWAYKEGG LinkMetaCyc Link
No entries in this field

SYSTEMATIC NAMEIUBMB Comments
L-methionine:glyoxylate aminotransferaseL-Glutamate can also act as donor.

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
aminotransferase, methionine-glyoxylate----
methionine-glyoxylate aminotransferase----
MGAT----

CAS REGISTRY NUMBERCOMMENTARY
116155-75-0-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Brassica carinatavar. R-4218636989, 636990--Manually annotated by BRENDA team
Brassica napusvar. Topas636990--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
L-glutamine + 4-methylsulfanyl-2-oxobutanoate2-oxoglutarate + L-methionine
show the reaction diagram		Brassica napus, Brassica carinata--636990--?
L-glutamine + 4-methylsulfanyl-2-oxobutanoate2-oxoglutarate + L-methionine
show the reaction diagram		Brassica carinata-187% of activity compared to glyoxylate636989--?
L-glutamine + glyoxylate2-oxoglutarate + glycine
show the reaction diagram		Brassica napus, Brassica carinata--636990--?
L-glutamine + glyoxylate2-oxoglutarate + glycine
show the reaction diagram		Brassica carinata-as good as L-methionine636989--?
L-histidine + glyoxylate3-(1H-imidazol-4-yl)-2-oxopropanoate + glycine
show the reaction diagram		Brassica carinata-16% of activity compared to L-methionine636989--?
L-homoserine + glyoxylate4-hydroxy-2-oxobutanoate + glycine
show the reaction diagram		Brassica carinata-56% of activity compared to L-methionine636989--?
L-methionine + 2-oxoglutarate4-methylsulfanyl-2-oxobutanoate + L-glutamate
show the reaction diagram		Brassica carinata-19% of activity compared to glyoxylate636989--?
L-methionine + 4-methylsulfanyl-2-oxobutanoate4-methylsulfanyl-2-oxobutanoate + L-methionine
show the reaction diagram		Brassica napus, Brassica carinata--636990--?
L-methionine + 4-methylsulfanyl-2-oxobutanoate4-methylsulfanyl-2-oxobutanoate + L-methionine
show the reaction diagram		Brassica carinata-90% of activity compared to glyoxylate636989--?
L-methionine + glyoxylate4-methylsulfanyl-2-oxobutanoate + glycine
show the reaction diagram		Brassica napus--636990--?
L-methionine + glyoxylate4-methylsulfanyl-2-oxobutanoate + glycine
show the reaction diagram		Brassica carinata--636989-636989?
L-methionine + glyoxylate4-methylsulfanyl-2-oxobutanoate + glycine
show the reaction diagram		Brassica carinata--636990--?
L-methionine + oxaloacetate4-methylsulfanyl-2-oxobutanoate + L-aspartate
show the reaction diagram		Brassica carinata--636989--?
L-methionine + oxaloacetate4-methylsulfanyl-2-oxobutanoate + L-aspartate
show the reaction diagram		Brassica carinata-24% of activity compared to glyoxylate636990---
L-methionine + pyruvate4-methylsulfanyl-2-oxobutanoate + L-alanine
show the reaction diagram		Brassica carinata-35% of activity compared to glyoxylate636989--?
L-methionine sulfone + glyoxylate4-methylsulfonyl-2-oxobutanoate + glycine
show the reaction diagram		Brassica carinata-29% of activity compared to L-methionine636989--?
L-methionine sulfoxide + glyoxylate4-methylsulfinyl-2-oxobutanoate + glycine
show the reaction diagram		Brassica carinata-54% of activity compared to L-methionine636989--?
S-methyl-L-cysteine + glyoxylate3-methylsulfanyl-2-oxopropanoate + glycine
show the reaction diagram		Brassica carinata-49% of activity compared to L-methionine636989--?
L-methionine sulfoximine + glyoxylate(S-methylsulfonimidoyl)-2-oxobutanoate + glycine
show the reaction diagram		Brassica carinata-35% of activity compared to L-methionine636989--?
additional information?-Brassica napus, Brassica carinata-function in the deamination of methionine or production of methionine in the methionine-cycle636990---
additional information?-Brassica carinata-involved in the biosynthesis of methionine-derived glucosinolates636989, 636990---

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
additional information?-Brassica napus, Brassica carinata-function in the deamination of methionine or production of methionine in the methionine-cycle636990--
additional information?-Brassica carinata-involved in the biosynthesis of methionine-derived glucosinolates636989, 636990--

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
No entries in this field

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
No entries in this field

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
0.06-Brassica carinata-isoenzyme MGAT 3, 30°C, pH 8.2636990
0.066-Brassica napus-MGAT, single enzyme 30°C, pH 8.2636990
0.18-Brassica carinata-isoenzyme MGAT 2 30°C, pH 8.2636990
0.6-Brassica carinata-isoenzyme MGAT 1, overall activity without separation of isoenzymes 30°C, pH 8.2636990

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
No entries in this field

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
30-Brassica carinata-assay at636989, 636990
30-Brassica napus-assay at636990

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
leafBrassica carinata--636989, 636990Manually annotated by BRENDA team
leafBrassica napus--636990Manually annotated by BRENDA team

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
No entries in this field

PDBSCOPCATHORGANISM
No entries in this field

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
230000290000Brassica carinata, Brassica napus-gel filtration636990

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
?Brassica carinata-x * 49000, SDS-PAGE636990
?Brassica napus-x * 51000, SDS-PAGE636990

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
No entries in this field

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-60°C, desalted enzyme preparationBrassica carinata, Brassica napus-636990

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
purification on Mono Q results in isolation of three isoenzymes, MGAT 1, MGAT 2 and MGAT 3; var. R-4218, partialBrassica carinata-636990
var. R-4218, partialBrassica carinata-636989
var. Topas, partialBrassica napus-636990

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISMLINK TO PUBMEDSOURCE
636989Glover, J.R.; Chapple, C.C.S.; Rothwell, S.; Tober, I.; Ellis, B.E.Allylglucosinolate biosynthesis in Brassica carinataPhytochemistry271345-13481988Brassica carinata-
636990Chapple, C.C.S.; Glover, J.R.; Ellis, B.E.Purification and characterization of methionine:glyoxylate aminotransferase from Brassica carinata and Brassica napusPlant Physiol.941887-18961990Brassica carinata, Brassica napus PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 2.6.1.73)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)