Information on EC 2.6.1.41 - D-methionine-pyruvate transaminase:
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The lowest common taxonomy group for this enzyme is: rosids

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EC NUMBERCOMMENTARY
2.6.1.41-

RECOMMENDED NAMEGeneOntology No.
D-methionine-pyruvate transaminaseGO:0047306

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
D-methionine + pyruvate = 4-methylthio-2-oxobutanoate + L-alanine
show the reaction diagram		----

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
amino group transfer----

PATHWAYKEGG LinkMetaCyc Link
No entries in this field

SYSTEMATIC NAMEIUBMB Comments
D-methionine:pyruvate aminotransferaseOxaloacetate can replace pyruvate.

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
aminotransferase, D-methionine----
D-methionine aminotransferase----
D-methionine transaminase----

CAS REGISTRY NUMBERCOMMENTARY
37277-93-3-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Arachis hypogaeapeanut cv. Starr636703--Manually annotated by BRENDA team
Brassica sp.cauliflower636704--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
D-methionine + 2-oxoglutarate4-methylthio-2-oxobutanoate + L-glutamate
show the reaction diagram		Arachis hypogaea-less effective than pyruvate636703-636703?
D-methionine + 2-oxoglutarate4-methylthio-2-oxobutanoate + L-glutamate
show the reaction diagram		Brassica sp.-less effective than pyruvate636704-636704?
D-methionine + oxaloacetate4-methylthio-2-oxobutanoate + L-aspartate
show the reaction diagram		Arachis hypogaea-less effective than pyruvate636703-636703?
D-methionine + oxaloacetate4-methylthio-2-oxobutanoate + L-aspartate
show the reaction diagram		Brassica sp.-less effective than pyruvate636704-636704?
D-methionine + pyruvate4-methylthio-2-oxobutanoate + L-alanine
show the reaction diagram		Arachis hypogaea--636703-636703?
D-methionine + pyruvate4-methylthio-2-oxobutanoate + L-alanine
show the reaction diagram		Brassica sp.--636704-636704?
D-methionine + pyruvate4-methylthio-2-oxobutanoate + L-alanine
show the reaction diagram		Brassica sp.-involved in ethylene synthesis from methionine636704---
L-methionine + pyruvate4-methylthio-2-oxobutanoate + L-alanine
show the reaction diagram		Brassica sp.-one third of activity compared to D-methionine636704-636704?

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
D-methionine + pyruvate4-methylthio-2-oxobutanoate + L-alanine
show the reaction diagram		Brassica sp.-involved in ethylene synthesis from methionine636704--

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
pyridoxal 5'-phosphateArachis hypogaea-required for full enzyme activity636703 2D-image

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
No entries in this field

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
additional information-Arachis hypogaea-10061 units/mg, 1 unit is defined as the quantity of enzyme that produces 1 nanoliter of ethylene per h of dark reaction636703

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
7.5-Brassica sp.--636704
7.8-Arachis hypogaea-assay at636703

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
68Brassica sp.-15% of maximal activity at pH 6.0, 66% of maximal activity at pH 8.0636704

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
25-Brassica sp.--636704
31-Arachis hypogaea-assay at636703

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
embryoArachis hypogaea-germinating636703Manually annotated by BRENDA team
floretBrassica sp.--636704Manually annotated by BRENDA team

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
mitochondrionBrassica sp.--5739636704Manually annotated by BRENDA team

PDBSCOPCATHORGANISM
No entries in this field

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
No entries in this field

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
pyridoxal 5'-phosphate stabilizesArachis hypogaea-636703
reducing agents, such as cysteine, 2-mercaptoethanol or dithiothreitol, are essential for maintaining activity during purificationArachis hypogaea-636703

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
ammonium sulfate, cold precipitation, acetone precipitation, partial purificationArachis hypogaea-636703
partialBrassica sp.-636704

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISMLINK TO PUBMEDSOURCE
636703Durham, J.I.; Morgan, P.W.; Prescott, J.M.; Lyman, C.M.An aminotransferase specific for the D-enantiomorph of methioninePhytochemistry122123-21261973Arachis hypogaea-
636704Mapson, L.W.; March, J.F.; Wardale, D.A.Biosynthesis of ethylene. 4-methylmercapto-2-oxobutyric acid: an intermediate in the formation from methionineBiochem. J.115653-6611969Brassica sp. PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 2.6.1.41)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)