Information on EC 2.6.1.29 - diamine transaminase:
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The expected taxonomic range for this enzyme is: Amycolatopsis lactamdurans

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EC NUMBERCOMMENTARY
2.6.1.29-

RECOMMENDED NAMEGeneOntology No.
diamine transaminaseGO:0019161

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
an alpha,omega-diamine + 2-oxoglutarate = an omega-aminoaldehyde + L-glutamate
show the reaction diagram		----

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
amino group transfer----

PATHWAYKEGG LinkMetaCyc Link
Arginine and proline metabolism00330 -

SYSTEMATIC NAMEIUBMB Comments
diamine:2-oxoglutarate aminotransferase-

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
amine transaminase----
amine-ketoacid transaminase----
aminotransferase, diamine----
diamine aminotransferase----
diamine-ketoglutaric transaminase----

CAS REGISTRY NUMBERCOMMENTARY
9031-83-8-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Amycolatopsis lactamduransstrain NRRL 3802639953--Manually annotated by BRENDA team
Amycolatopsis lactamdurans NRRLstrain NRRL 3802639953--Manually annotated by BRENDA team
Escherichia coli-639950, 639951--Manually annotated by BRENDA team
Streptomyces clavuligerus-639952--Manually annotated by BRENDA team
Streptomyces clavuligerusstrain NRRL 3585639953--Manually annotated by BRENDA team
Streptomyces clavuligerus NRRL 3585strain NRRL 3585639953--Manually annotated by BRENDA team
Streptomyces glaucescensstrain GLAO639953--Manually annotated by BRENDA team
Streptomyces glaucescens GLAOstrain GLAO639953--Manually annotated by BRENDA team
Streptomyces griseusstrain NRRL 3851639953--Manually annotated by BRENDA team
Streptomyces griseus NRRL 3851strain NRRL 3851639953--Manually annotated by BRENDA team
Streptomyces lividansstrain TK24639953--Manually annotated by BRENDA team
Streptomyces parvulusstrain ISP5048639953--Manually annotated by BRENDA team
Streptomyces parvulus ISP5048strain ISP5048639953--Manually annotated by BRENDA team
Streptomyces phaeochromogenesstrain B2196639953--Manually annotated by BRENDA team
Streptomyces phaeochromogenes B2196strain B2196639953--Manually annotated by BRENDA team
Streptomyces rimosusstrain NRRL 2234639953--Manually annotated by BRENDA team
Streptomyces rimosus NRRL 2234strain NRRL 2234639953--Manually annotated by BRENDA team
Streptomyces venezuelaestrain ISP5230639953--Manually annotated by BRENDA team
Streptomyces venezuelae ISP5230strain ISP5230639953--Manually annotated by BRENDA team
Streptomyces viridochromogenesstrain CUB416639953--Manually annotated by BRENDA team
Streptomyces viridochromogenes CUB416strain CUB416639953--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
(+)-1,4-diaminobutan-2-ol + 2-oxoglutarate?
show the reaction diagram		Escherichia coli-30% of the activity with putrescine639951---
1,6-diaminohexane + 2-oxoglutarate6-aminohexanal + L-glutamate
show the reaction diagram		Escherichia coli--639951-639951?
1,7-diaminoheptane + 2-oxoglutarate7-aminoheptanal + L-glutamate
show the reaction diagram		Escherichia coli-30% of the activity with putrescine639950-639950?
1,7-diaminoheptane + 2-oxoglutarate7-aminoheptanal + L-glutamate
show the reaction diagram		Escherichia coli-30% of the activity with putrescine639951-639951?
4-aminobutanoate + 2-oxoglutarate4-oxobutanoate + L-glutamate
show the reaction diagram		Escherichia coli--639951-639951?
4-aminobutanoate + 2-oxoglutarate4-oxobutanoate + L-glutamate
show the reaction diagram		Escherichia coli-11% of the activity with putrescine639950-639950?
cadaverine + 2-oxoglutarate5-aminopentanal + L-glutamate
show the reaction diagram		Streptomyces griseus, Streptomyces phaeochromogenes, Streptomyces venezuelae, Streptomyces glaucescens, Streptomyces rimosus, Streptomyces lividans, Amycolatopsis lactamdurans, Streptomyces viridochromogenes, Streptomyces parvulus, Streptomyces clavuligerus--639953-639953?
cadaverine + 2-oxoglutarate5-aminopentanal + L-glutamate
show the reaction diagram		Escherichia coli-equally active as putrescine639950-639950?
cadaverine + 2-oxoglutarate5-aminopentanal + L-glutamate
show the reaction diagram		Escherichia coli-equally active as putrescine639951-639951?
cadaverine + 2-oxoglutarate5-aminopentanal + L-glutamate
show the reaction diagram		Streptomyces griseus, Streptomyces phaeochromogenes, Streptomyces venezuelae, Streptomyces glaucescens, Streptomyces rimosus, Streptomyces lividans, Amycolatopsis lactamdurans, Streptomyces viridochromogenes, Streptomyces parvulus, Streptomyces clavuligerus-enzyme catalyzes the second step in lysine catabolism639953-639953?
cadaverine + 2-oxoglutarate5-aminopentanal + L-glutamate
show the reaction diagram		Streptomyces viridochromogenes CUB416-enzyme catalyzes the second step in lysine catabolism639953-639953?
cadaverine + 2-oxoglutarate5-aminopentanal + L-glutamate
show the reaction diagram		Streptomyces viridochromogenes Tu494-enzyme catalyzes the second step in lysine catabolism639953-639953?
cadaverine + 2-oxoglutarate5-aminopentanal + L-glutamate
show the reaction diagram		Streptomyces viridochromogenes Tue494-enzyme catalyzes the second step in lysine catabolism639953-639953?
putrescine + 2-oxoglutarate4-aminobutanal + L-glutamate
show the reaction diagram		Escherichia coli--639950-639950?
putrescine + 2-oxoglutarate4-aminobutanal + L-glutamate
show the reaction diagram		Escherichia coli--639951-639951?
putrescine + pyruvate4-aminobutanal + L-alanine
show the reaction diagram		Escherichia coli--639950-639950?
putrescine + pyruvate4-aminobutanal + L-alanine
show the reaction diagram		Escherichia coli--639951-639951?
cadaverine + 2-oxoglutarate5-aminopentanal + L-glutamate
show the reaction diagram		Streptomyces viridochromogenes strain Tu494-enzyme catalyzes the second step in lysine catabolism639953-639953?
additional information?-Escherichia coli-inactive with oxaloacetate, inactive with 1,3-diaminopropane, lysine, ornithine, spermidine639950---
additional information?-Escherichia coli-inactive with 1,3-diaminopropane, lysine, ornithine, spermidine, inactive with histamine, tyramine, 4-aminobutanol, beta-alanine639951---

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
cadaverine + 2-oxoglutarate5-aminopentanal + L-glutamate
show the reaction diagram		Streptomyces griseus, Streptomyces phaeochromogenes, Streptomyces venezuelae, Streptomyces glaucescens, Streptomyces rimosus, Streptomyces lividans, Amycolatopsis lactamdurans, Streptomyces viridochromogenes, Streptomyces parvulus, Streptomyces clavuligerus, Streptomyces viridochromogenes CUB416, Streptomyces viridochromogenes Tu494, Streptomyces viridochromogenes Tue494, Streptomyces viridochromogenes strain Tu494-enzyme catalyzes the second step in lysine catabolism639953-639953
putrescine + 2-oxoglutarate4-aminobutanal + L-glutamate
show the reaction diagram		Escherichia coli--639950-639950
putrescine + 2-oxoglutarate4-aminobutanal + L-glutamate
show the reaction diagram		Escherichia coli--639951-639951

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
pyridoxal 5'-phosphateEscherichia coli-required639950 2D-image
pyridoxal 5'-phosphateEscherichia coli-purified enzyme normally active without added pyridoxal phosphate, absolute requirement when irradiated with UV light or treated with 2 mM phenylhydrazine and then with Norite639951 2D-image

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
No entries in this field

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
cyanideEscherichia coli-complete inhibition at 0.5 mM639951 2D-image
hydroxylamineEscherichia coli-complete inhibition at 0.5 mM639951 2D-image
SemicarbazideEscherichia coli-complete inhibition at 0.5 mM639951 2D-image

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.88-2-oxoglutarateEscherichia coli-pH 7.6, room temperature639950, 639951 2D-image
2.7-pyruvateEscherichia coli-pH 7.6, room temperature639950, 639951 2D-image

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
0.00064-Amycolatopsis lactamdurans, Streptomyces parvulus, Streptomyces parvulus ISP5048--639953
0.0011-Streptomyces rimosus--639953
0.00116-Streptomyces glaucescens, Streptomyces glaucescens ETH 22794, Streptomyces glaucescens GLA.0, Streptomyces glaucescens GLAO--639953
0.00156-Streptomyces venezuelae, Streptomyces venezuelae DOSf, Streptomyces venezuelae GY1, Streptomyces venezuelae ISP5230, Streptomyces venezuelae P10--639953
0.00167-Streptomyces viridochromogenes, Streptomyces viridochromogenes CUB416, Streptomyces viridochromogenes strain Tu494, Streptomyces viridochromogenes Tu494, Streptomyces viridochromogenes Tue494--639953
0.00192-Streptomyces griseus--639953
0.0021-Streptomyces lividans--639953
0.00238-Streptomyces clavuligerus--639953
0.00248-Streptomyces phaeochromogenes--639953
1.63-Escherichia coli--639950, 639951
additional information-Streptomyces clavuligerus-values with different nitrogen sources639952

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
910Escherichia coli--639950, 639951

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
810.5Escherichia coli-almost completely inactive at pH 7, pH 8 about 40% of maximum activity, pH 10.5 about 20% of maximum activity639950

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
37-Amycolatopsis lactamdurans, Amycolatopsis lactamdurans LC411, Amycolatopsis lactamdurans MA4213, Amycolatopsis lactamdurans NRRL3802, Amycolatopsis lactamdurans NRRL, Streptomyces clavuligerus, Streptomyces glaucescens, Streptomyces glaucescens ETH 22794, Streptomyces glaucescens GLA.0, Streptomyces glaucescens GLAO, Streptomyces griseus, Streptomyces lividans, Streptomyces parvulus, Streptomyces parvulus ISP5048, Streptomyces phaeochromogenes, Streptomyces phaeochromogenes B2196, Streptomyces phaeochromogenes NRRL B-3559, Streptomyces phaeochromogenes RRL B-3559, Streptomyces rimosus 4018, Streptomyces rimosus, Streptomyces rimosus ATCC10970, Streptomyces rimosus K-OK-02, Streptomyces rimosus LG-10, Streptomyces rimosus NRRL 2234, Streptomyces rimosus P, Streptomyces venezuelae, Streptomyces venezuelae DOSf, Streptomyces venezuelae GY1, Streptomyces venezuelae ISP5230, Streptomyces venezuelae P10, Streptomyces viridochromogenes, Streptomyces viridochromogenes CUB416, Streptomyces viridochromogenes strain Tu494, Streptomyces viridochromogenes Tu494, Streptomyces viridochromogenes Tue494-assay at639953

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
No entries in this field

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
No entries in this field

PDBSCOPCATHORGANISM
No entries in this field

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
No entries in this field

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-15ºC, 0.03 M Tris-HCl buffer, pH 7.6, 1 monthEscherichia coli-639951

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
precipitation with ammonium sulphate followed by column chromatographyEscherichia coli-639950, 639951

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISM (UNIPROT ACCESSION NO.)LINK TO PUBMEDSOURCE
639950Kim, K.H.Purification and properties of a diamine alpha-ketoglutarate transaminase from Escherichia coliJ. Biol. Chem.239783-7861964Escherichia coli PubMed
639951Kim, K.H.; Tchen, T.T.Diamine-alpha-ketoglutarate aminotransferase (Escherichia coli)Methods Enzymol.17B812-8151971Escherichia coli-
639952Bascarán, V.; Sánchez, L.; Hardisson, C.; Braña, A.F.Stringent response and initiation of secondary metabolism in Streptomyces clavuligerusJ. Gen. Microbiol.1371625-16341991Streptomyces clavuligerus PubMed
639953Madduri, K.; Stuttard, C.; Vining, L.C.Lysine catabolism in Streptomyces spp. is primarily through cadaverine: beta-lactam producers also make alpha-aminoadipateJ. Bacteriol.171299-3021989Amycolatopsis lactamdurans, Amycolatopsis lactamdurans NRRL, Streptomyces clavuligerus, Streptomyces clavuligerus NRRL 3585, Streptomyces glaucescens, Streptomyces glaucescens GLAO, Streptomyces griseus, Streptomyces griseus NRRL 3851, Streptomyces lividans, Streptomyces parvulus, Streptomyces parvulus ISP5048, Streptomyces phaeochromogenes, Streptomyces phaeochromogenes B2196, Streptomyces rimosus, Streptomyces rimosus NRRL 2234, Streptomyces venezuelae, Streptomyces venezuelae ISP5230, Streptomyces viridochromogenes, Streptomyces viridochromogenes CUB416 PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 2.6.1.29)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)