Information on EC 2.5.1.85 - all-trans-nonaprenyl diphosphate synthase [geranylgeranyl-diphosphate specific]:
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The expected taxonomic range for this enzyme is: Eukaryota

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EC NUMBERCOMMENTARY
2.5.1.85-

RECOMMENDED NAMEGeneOntology No.
all-trans-nonaprenyl diphosphate synthase [geranylgeranyl-diphosphate specific]GO:0052924

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
geranylgeranyl diphosphate + 5 isopentenyl diphosphate = 5 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram		-Arabidopsis thaliana-637968
geranylgeranyl diphosphate + 5 isopentenyl diphosphate = 5 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram		----

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

PATHWAYKEGG LinkMetaCyc Link
Biosynthesis of secondary metabolites01110 -
nonaprenyl diphosphate biosynthesis II-PWY-6520
Terpenoid backbone biosynthesis00900 -

SYSTEMATIC NAMEIUBMB Comments
geranylgeranyl-diphosphate:isopentenyl-diphosphate transtransferase (adding 5 isopentenyl units)Geranylgeranyl diphosphate is preferred over farnesyl diphosphate as allylic substrate [1]. The plant Arabidopsis thaliana has two different enzymes that catalyse this reaction. SPS1 contributes to the biosynthesis of the ubiquinone side-chain while SPS2 supplies the precursor of the plastoquinone side-chains [2].

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
At-SPS1Arabidopsis thaliana--637968, 658312
At-SPS2Arabidopsis thaliana--658312
HbSDSHevea brasiliensis--676675
solanesyl diphosphate synthaseArabidopsis thaliana-; 660147
solanesyl-diphosphate synthaseTrypanosoma cruziQ964Q8-674727
SPPSTrypanosoma cruziQ964Q8-674727
SPS1Arabidopsis thaliana--660147
Sps2Arabidopsis thaliana--660147
TCSPPSTrypanosoma cruziQ964Q8-674727

CAS REGISTRY NUMBERCOMMENTARY
83745-07-7-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Arabidopsis thaliana-637968, 658312, 660147Q8S948SwissProtManually annotated by BRENDA team
Arabidopsis thaliana-658312, 660147Q76FS5SwissProtManually annotated by BRENDA team
Hevea brasiliensis-676675--Manually annotated by BRENDA team
Trypanosoma cruzi-674727Q964Q8SwissProtManually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
(2E,6E)-farnesyl diphosphate + 6 isopentenyl diphosphate6 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram		Trypanosoma cruziQ964Q8-674727--?
(2E,6E)-farnesyl diphosphate + 6 isopentenyl diphosphate6 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram		Arabidopsis thalianaQ76FS5At-SPS2 prefers geranylgeranyl diphosphate to farnesyl diphosphate as the allylic substrate658312the main product is nonaprenyl diphosphate, considerable amounts of intermediates, such as geranylgeranyl diphosphate are formed-?
(2E,6E)-farnesyl diphosphate + 6 isopentenyl diphosphate6 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram		Arabidopsis thalianaQ8S948geranylgeranyl diphosphate is preferred over farnesyl diphosphate as allylic substrate637968the main product is nonaprenyl diphosphate, considerable amounts of geranylgeranyl diphosphate are formed-?
(2E,6E)-farnesyl diphosphate + 6 isopentenyl diphosphate6 diphosphate + nonaprenyl diphosphate
show the reaction diagram		Hevea brasiliensis-17% of the activity with geranylgeranyl diphosphate676675--?
dimethylallyl diphosphate + 8 isopentenyl diphosphate8 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram		Trypanosoma cruziQ964Q8low activity674727--?
geranyl diphosphate + 7 isopentenyl diphosphate7 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram		Trypanosoma cruziQ964Q8-674727--?
geranyl diphosphate + 7 isopentenyl diphosphate7 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram		Hevea brasiliensis-39% of the activity with geranylgeranyl diphosphate676675--?
geranylgeranyl diphosphate + 5 isopentenyl diphosphate5 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram		Hevea brasiliensis-geranylgeranyl diphosphate is the preferred allylic substrate676675--?
geranylgeranyl diphosphate + 5 isopentenyl diphosphate5 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram		Trypanosoma cruziQ964Q8geranylgeranyl diphosphate is the preferred allylic substrate674727--?
geranylgeranyl diphosphate + 5 isopentenyl diphosphate5 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram		Arabidopsis thaliana-At-SPS1 contributes to the biosynthesis of the plastoquinone side-chain in Arabidopsis thaliana660147--?
geranylgeranyl diphosphate + 5 isopentenyl diphosphate5 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram		Arabidopsis thalianaQ76FS5At-SPS1 contributes to the biosynthesis of the ubiquinone side-chain in Arabidopsis thaliana658312--?
geranylgeranyl diphosphate + 5 isopentenyl diphosphate5 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram		Arabidopsis thaliana-At-SPS1 contributes to the biosynthesis of the ubiquinone side-chain in Arabidopsis thaliana660147--?
geranylgeranyl diphosphate + 5 isopentenyl diphosphate5 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram		Arabidopsis thalianaQ76FS5At-SPS2 supplies the precursor of the plastoquinone side-chain in Arabidopsis thaliana658312--?
geranylgeranyl diphosphate + 5 isopentenyl diphosphate5 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram		Trypanosoma cruziQ964Q8the enzyme is involved in synthesis of ubiquinone-9674727--?
geranylgeranyl diphosphate + 5 isopentenyl diphosphate5 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram		Arabidopsis thalianaQ76FS5At-SPS2 prefers geranylgeranyl diphosphate to farnesyl diphosphate as the allylic substrate658312a large amount of nonaprenyl diphosphate is formed-?
geranylgeranyl diphosphate + 5 isopentenyl diphosphate5 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram		Arabidopsis thalianaQ8S948geranylgeranyl diphosphate is preferred over farnesyl diphosphate as allylic substrate637968nonaprenyl diphosphate is almost exclusively formed-?
additional information?-Arabidopsis thalianaQ76FS5no activity with dimethylallyl diphosphate and geranyl diphosphate, recombinant His6-tagged At-SPS2 fusion protein and truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus)658312---
additional information?-Arabidopsis thalianaQ8S948no activity with farnesyl diphosphate and dimethylallyl diphosphate637968---

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
geranylgeranyl diphosphate + 5 isopentenyl diphosphate5 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram		Arabidopsis thaliana-At-SPS1 contributes to the biosynthesis of the plastoquinone side-chain in Arabidopsis thaliana660147--
geranylgeranyl diphosphate + 5 isopentenyl diphosphate5 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram		Arabidopsis thalianaQ76FS5At-SPS1 contributes to the biosynthesis of the ubiquinone side-chain in Arabidopsis thaliana658312--
geranylgeranyl diphosphate + 5 isopentenyl diphosphate5 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram		Arabidopsis thaliana-At-SPS1 contributes to the biosynthesis of the ubiquinone side-chain in Arabidopsis thaliana660147--
geranylgeranyl diphosphate + 5 isopentenyl diphosphate5 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram		Arabidopsis thalianaQ76FS5At-SPS2 supplies the precursor of the plastoquinone side-chain in Arabidopsis thaliana658312--
geranylgeranyl diphosphate + 5 isopentenyl diphosphate5 diphosphate + all-trans-nonaprenyl diphosphate
show the reaction diagram		Trypanosoma cruziQ964Q8the enzyme is involved in synthesis of ubiquinone-9674727--

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
No entries in this field

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
Mg2+Trypanosoma cruziQ964Q8optimal level of activity obtained at 0.5-1 mM. No activation by Mn2+674727

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.00689-(2E,6E)-farnesyl diphosphateArabidopsis thaliana-pH 8.0, 30°C, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus)658312 2D-image
5.73-(2E,6E)-farnesyl diphosphateArabidopsis thaliana-pH 8.0, 30°C637968 2D-image
0.00459-farnesyl diphosphateTrypanosoma cruziQ964Q8pH 7.4, 37°C674727 2D-image
0.00512-farnesyl diphosphateArabidopsis thaliana-pH 8.0, 30°C, His6-tagged At-SPS2 fusion protein658312 2D-image
0.0548-geranyl diphosphateTrypanosoma cruziQ964Q8pH 7.4, 37°C674727 2D-image
0.000565-geranylgeranyl diphosphateArabidopsis thaliana-pH 8.0, 30°C, His6-tagged At-SPS2 fusion protein658312 2D-image
0.000843-geranylgeranyl diphosphateArabidopsis thaliana-pH 8.0, 30°C, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus), truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus)658312 2D-image
0.00707-geranylgeranyl diphosphateTrypanosoma cruziQ964Q8pH 7.4, 37°C674727 2D-image
1.61-geranylgeranyl diphosphateArabidopsis thaliana-pH 8.0, 30°C637968 2D-image
0.0192-isopentenyl diphosphateTrypanosoma cruziQ964Q8pH 7.4, 37°C, cosubstrate: geranyl diphosphate674727 2D-image
0.0243-isopentenyl diphosphateTrypanosoma cruziQ964Q8pH 7.4, 37°C, cosubstrate: geranylgeranyl diphosphate674727 2D-image
0.0289-isopentenyl diphosphateArabidopsis thaliana-pH 8.0, 30°C, cosubstrate: geranylgeranyl diphosphate, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus)658312 2D-image
0.0307-isopentenyl diphosphateTrypanosoma cruziQ964Q8pH 7.4, 37°C, cosubstrate: farnesyl diphosphate674727 2D-image
0.0377-isopentenyl diphosphateArabidopsis thaliana-pH 8.0, 30°C, cosubstrate: geranylgeranyl diphosphate, His6-tagged At-SPS2 fusion protein658312 2D-image
0.182-isopentenyl diphosphateArabidopsis thaliana-pH 8.0, 30°C, cosubstrate: farnesyl diphosphate, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus)658312 2D-image
0.248-isopentenyl diphosphateArabidopsis thaliana-pH 8.0, 30°C, cosubstrate: farnesyl diphosphate, His6-tagged At-SPS2 fusion protein658312 2D-image
20-isopentenyl diphosphateArabidopsis thaliana-pH 8.0, 30°C, cosubstrate: geranylgeranyl diphosphate637968 2D-image
151-isopentenyl diphosphateArabidopsis thaliana-pH 8.0, 30°C, cosubstrate: farnesyl diphosphate637968 2D-image

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.032-(2E,6E)-farnesyl diphosphateTrypanosoma cruziQ964Q8pH 7.4, 37°C674727 2D-image
3.77-(2E,6E)-farnesyl diphosphateArabidopsis thaliana-pH 8.0, 30°C, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus)658312 2D-image
29.7-farnesyl diphosphateArabidopsis thaliana-pH 8.0, 30°C637968 2D-image
0.065-geranyl diphosphateTrypanosoma cruziQ964Q8pH 7.4, 37°C674727 2D-image
0.087-geranylgeranyl diphosphateTrypanosoma cruziQ964Q8pH 7.4, 37°C674727 2D-image
2.33-geranylgeranyl diphosphateArabidopsis thaliana-pH 8.0, 30°C, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus), truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus)658312 2D-image
21-geranylgeranyl diphosphateArabidopsis thaliana-pH 8.0, 30°C637968 2D-image
0.043-isopentenyl diphosphateTrypanosoma cruziQ964Q8pH 7.4, 37°C, cosubstrate: farnesyl diphosphate674727 2D-image
0.131-isopentenyl diphosphateTrypanosoma cruziQ964Q8pH 7.4, 37°C, cosubstrate: geranyl diphosphate674727 2D-image
0.217-isopentenyl diphosphateTrypanosoma cruziQ964Q8pH 7.4, 37°C, cosubstrate: geranylgeranyl diphosphate674727 2D-image
1.72-isopentenyl diphosphateArabidopsis thaliana-pH 8.0, 30°C, cosubstrate: geranylgeranyl diphosphate, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus)658312 2D-image
2.83-isopentenyl diphosphateArabidopsis thaliana-pH 8.0, 30°C, cosubstrate: farnesyl diphosphate, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus)658312 2D-image
14.7-isopentenyl diphosphateArabidopsis thaliana-pH 8.0, 30°C, cosubstrate: farnesyl diphosphate637968 2D-image
15-isopentenyl diphosphateArabidopsis thaliana-pH 8.0, 30°C, cosubstrate: geranylgeranyl diphosphate637968 2D-image

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.00701-(2E,6E)-farnesyl diphosphateTrypanosoma cruziQ964Q8pH 7.4, 37°C674727263972
5.19-(2E,6E)-farnesyl diphosphateArabidopsis thaliana-pH 8.0, 30°C637968263972
547-(2E,6E)-farnesyl diphosphateArabidopsis thaliana-pH 8.0, 30°C, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus)658312263972
0.00119-geranyl diphosphateTrypanosoma cruziQ964Q8pH 7.4, 37°C67472710850
0.012-geranylgeranyl diphosphateTrypanosoma cruziQ964Q8pH 7.4, 37°C67472710855
13.1-geranylgeranyl diphosphateArabidopsis thaliana-pH 8.0, 30°C63796810855
2770-geranylgeranyl diphosphateArabidopsis thaliana-pH 8.0, 30°C, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus), truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus)65831210855
0.0014-isopentenyl diphosphateTrypanosoma cruziQ964Q8pH 7.4, 37°C, cosubstrate: farnesyl diphosphate67472711832
0.00685-isopentenyl diphosphateTrypanosoma cruziQ964Q8pH 7.4, 37°C, cosubstrate: geranyl diphosphate67472711832
0.00893-isopentenyl diphosphateTrypanosoma cruziQ964Q8pH 7.4, 37°C, cosubstrate: geranylgeranyl diphosphate67472711832
0.0968-isopentenyl diphosphateArabidopsis thaliana-pH 8.0, 30°C, cosubstrate: farnesyl diphosphate63796811832
0.752-isopentenyl diphosphateArabidopsis thaliana-pH 8.0, 30°C, cosubstrate: geranylgeranyl diphosphate63796811832
15.5-isopentenyl diphosphateArabidopsis thaliana-pH 8.0, 30°C, cosubstrate: farnesyl diphosphate, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus)65831211832
595-isopentenyl diphosphateArabidopsis thaliana-pH 8.0, 30°C, cosubstrate: geranylgeranyl diphosphate, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus)65831211832

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
8-Arabidopsis thaliana--637968

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
6.01-Trypanosoma cruziQ964Q8calculated from sequence674727

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
epimastigoteTrypanosoma cruziQ964Q8-674727Manually annotated by BRENDA team
latexHevea brasiliensis-high activity676675Manually annotated by BRENDA team
leafArabidopsis thaliana-mRNA level is 11fold higher in leaf than in root; mRNA level is 5fold higher in leaf than in root658312Manually annotated by BRENDA team
leafHevea brasiliensis--676675Manually annotated by BRENDA team
rootArabidopsis thaliana-mRNA level is 11fold higher in leaf than in root; mRNA level is 5fold higher in leaf than in root658312Manually annotated by BRENDA team
rootHevea brasiliensis-low activity676675Manually annotated by BRENDA team
stemHevea brasiliensis-young676675Manually annotated by BRENDA team

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
chloroplastArabidopsis thaliana-At-SPS2 is transported into chloroplasts9507658312Manually annotated by BRENDA team
chloroplastArabidopsis thaliana--9507660147Manually annotated by BRENDA team
endoplasmic reticulumArabidopsis thaliana-At-SPS15783658312Manually annotated by BRENDA team
endoplasmic reticulumArabidopsis thaliana--5783660147Manually annotated by BRENDA team
glycosomeTrypanosoma cruziQ964Q8-20015674727Manually annotated by BRENDA team

PDBSCOPCATHORGANISM
No entries in this field

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
108000-Arabidopsis thaliana-gel filtration637968

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
?Arabidopsis thaliana-x * 46044, calculated from sequence658312
?Trypanosoma cruziQ964Q8x * 39000, calculated from sequence674727
?Hevea brasiliensis-x * 60000, His-tagged fusion protein, SDS-PAGE676675
dimerArabidopsis thaliana-2 * 46600, His6-tagged protein, calculated from sequence637968

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
40-Arabidopsis thaliana-pH 8.0, 10 minm, stable below637968

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-Arabidopsis thaliana-637968
recombinant His6-tagged At-SPS2 fusion protein partially purified to about 10% purity, truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus) is purified to about 90% purityArabidopsis thaliana-658312
-Trypanosoma cruziQ964Q8674727

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
expression in Escherichia coli, His6-tagged At-SPS2 fusion protein and the truncated from of At-SPS2 (At-SPS2DELTA, in which the 30 N-terminal residues of the putative signal-peptide are removed and, instead the His6 tag is attached to the N-terminus)Arabidopsis thaliana-658312
heterologous expression of either SPS1 allows the generation of UQ-9 in a decaprenyl diphosphate synthase-defective strain of fission yeast and also in wild-type Escherichia coli; heterologous expression of SPS1 allows the generation of UQ-9 in a decaprenyl diphosphate synthase-defective strain of fission yeast and also in wild-type Escherichia coliArabidopsis thaliana-660147
heterologously expressed in Escherichia coli as as His6-tagged fusion proteinArabidopsis thaliana-637968
expression in Escherichia coliHevea brasiliensis-676675
expression of TcSPPS is able to complement an Escherichia coli ispB mutantTrypanosoma cruziQ964Q8674727

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISM (UNIPROT ACCESSION NO.)LINK TO PUBMEDSOURCE
637968Hirooka, K.; Bamba, T.; Fukusaki, E.I.; Kobayashi, A.Cloning and kinetic characterization of Arabidopsis thaliana solanesyl diphosphate synthaseBiochem. J.370679-6862003Arabidopsis thaliana, Arabidopsis thaliana (Q8S948) PubMed
658312Hirooka, K.; Izumi, Y.; An, C.I.; Nakazawa, Y.; Fukusaki, E.; Kobayashi, A.Functional analysis of two solanesyl diphosphate synthases from Arabidopsis thalianaBiosci. Biotechnol. Biochem.69592-6012005Arabidopsis thaliana, Arabidopsis thaliana (Q76FS5), Arabidopsis thaliana (Q8S948) PubMed
660147Jun, L.; Saiki, R.; Tatsumi, K.; Nakagawa, T.; Kawamukai, M.Identification and subcellular localization of two solanesyl diphosphate synthases from Arabidopsis thalianaPlant Cell Physiol.451882-18882004Arabidopsis thaliana (Q76FS5), Arabidopsis thaliana (Q8S948) PubMed
674727Ferella, M.; Montalvetti, A.; Rohloff, P.; Miranda, K.; Fang, J.; Reina, S.; Kawamukai, M.; Bua, J.; Nilsson, D.; Pravia, C.; Katzin, A.; Cassera, M.B.; Aslund, L.; Andersson, B.; Docampo, R.; Bontempi, E.J.A solanesyl-diphosphate synthase localizes in glycosomes of Trypanosoma cruziJ. Biol. Chem.28139339-393482006Trypanosoma cruzi, Trypanosoma cruzi (Q964Q8) PubMed
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LINKS TO OTHER DATABASES (specific for EC-Number 2.5.1.85)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)