Information on EC 2.5.1.24 - discadenine synthase:
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The lowest common taxonomy group for this enzyme is: Dictyostelium

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EC NUMBERCOMMENTARY
2.5.1.24-

RECOMMENDED NAMEGeneOntology No.
discadenine synthaseGO:0047870

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
S-adenosyl-L-methionine + N6-(DELTA2-isopentenyl)-adenine = S-methyl-5'-thioadenosine + discadenine
show the reaction diagram		----

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
aminocarboxypropyl group transfer----

PATHWAYKEGG LinkMetaCyc Link
No entries in this field

SYSTEMATIC NAMEIUBMB Comments
S-adenosyl-L-methionine:N6-(DELTA2-isopentenyl)-adenine 3-(3-amino-3-carboxypropyl)-transferase-

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
discadenine synthetase----
synthase, discadenine----

CAS REGISTRY NUMBERCOMMENTARY
74082-52-3-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Dictyostelium discoideum-638424, 638425, 638426--Manually annotated by BRENDA team
Dictyostelium mucoroides-638426--Manually annotated by BRENDA team
Dictyostelium purpureum-638426--Manually annotated by BRENDA team
no activity in Dictyostelium minutum-638426--Manually annotated by BRENDA team
no activity in Polysphondylium pallidum-638426--Manually annotated by BRENDA team
no activity in Polysphondylium violaceum-638426--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + N6-(DELTA2-isopentenyl)-adenine5'-methylthioadenosine + discadenine
show the reaction diagram		Dictyostelium discoideum--638426--?
S-adenosyl-L-methionine + N6-(DELTA2-isopentenyl)-adenine5'-methylthioadenosine + discadenine
show the reaction diagram		Dictyostelium mucoroides--638426--?
S-adenosyl-L-methionine + N6-(DELTA2-isopentenyl)-adenine5'-methylthioadenosine + discadenine
show the reaction diagram		Dictyostelium purpureum--638426--?
S-adenosyl-L-methionine + N6-(DELTA2-isopentenyl)-adenine5'-methylthioadenosine + discadenine
show the reaction diagram		Dictyostelium discoideum--638424discadenine is 3-(3-amino-3-carboxypropyl)-N6-(DELTA2-isopentenyl)-adenine638424?
S-adenosyl-L-methionine + N6-(DELTA2-isopentenyl)-adenine5'-methylthioadenosine + discadenine
show the reaction diagram		Dictyostelium discoideum--638425discadenine is 3-(3-amino-3-carboxypropyl)-N6-(DELTA2-isopentenyl)-adenine638425?
S-adenosyl-L-methionine + N6-(DELTA2-isopentenyl)-adenine5'-methylthioadenosine + discadenine
show the reaction diagram		Dictyostelium discoideum-discadenine is produced as an inhibitor of spore germination in the species of cellular slime molds in which in which acrasin is cyclic adenosine 5'-monophosphate638426---
S-adenosyl-L-methionine + N6-(DELTA2-isopentenyl)-adenine5'-methylthioadenosine + discadenine
show the reaction diagram		Dictyostelium discoideum-biosynthesis of discadenine, an inhibitor of spore germination and a potent cytokinin638424---
S-adenosylmethionine + N6-benzyladenine3-(3-amino-3-carboxypropyl)-N6-benzyladenine + 5'-methylthioadenosine
show the reaction diagram		Dictyostelium discoideum--638425-638425?

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
S-adenosyl-L-methionine + N6-(DELTA2-isopentenyl)-adenine5'-methylthioadenosine + discadenine
show the reaction diagram		Dictyostelium mucoroides, Dictyostelium purpureum--638426--
S-adenosyl-L-methionine + N6-(DELTA2-isopentenyl)-adenine5'-methylthioadenosine + discadenine
show the reaction diagram		Dictyostelium discoideum-discadenine is produced as an inhibitor of spore germination in the species of cellular slime molds in which in which acrasin is cyclic adenosine 5'-monophosphate638426--
S-adenosyl-L-methionine + N6-(DELTA2-isopentenyl)-adenine5'-methylthioadenosine + discadenine
show the reaction diagram		Dictyostelium discoideum-biosynthesis of discadenine, an inhibitor of spore germination and a potent cytokinin638424--

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
No entries in this field

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
No entries in this field

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
Ca2+Dictyostelium discoideum--638425 2D-image
Mg2+Dictyostelium discoideum-100 mM, 30% inhibition638425 2D-image
Mn2+Dictyostelium discoideum--638425 2D-image
Zn2+Dictyostelium discoideum-100 mM, 80% inhibition638425 2D-image

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.0007-N6-benzyladenineDictyostelium discoideum--638425 2D-image
0.0185-S-adenosyl-L-methionineDictyostelium discoideum--638425 2D-image

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
0.0123-Dictyostelium discoideum--638425

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
7.5-Dictyostelium discoideum--638424, 638425

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
68.5Dictyostelium discoideum-pH 6.0: about 55% of activity maximum, pH 8.5: about 60% of activity maximum638425

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
35-Dictyostelium discoideum--638425

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
fruiting bodyDictyostelium discoideum--638425Manually annotated by BRENDA team

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
No entries in this field

PDBSCOPCATHORGANISM
No entries in this field

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
82000-Dictyostelium discoideum-gel filtration638425

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
No entries in this field

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-Dictyostelium discoideum-638425

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISMLINK TO PUBMEDSOURCE
638424Taya, Y.; Tanaka, Y.; Nishimura, S.Cell-free biosynthesis of discadenine, a spore germination inhibitor of Dictyostelium discoideumFEBS Lett.89326-3281978Dictyostelium discoideum PubMed
638425Ihara, M.; Tanaka, Y.; Yanagisawa, K.; Taya, Y.; Nishimura, S.Purification and some properties of discadenine synthase from Dictyostelium discoideumBiochim. Biophys. Acta881135-1401986Dictyostelium discoideum-
638426Taya, Y.; Yamada, T.; Nishimura, S.Correlation between acrasins and spore germination inhibitors in cellular slime moldsJ. Bacteriol.143715-7191980Dictyostelium discoideum, Dictyostelium mucoroides, Dictyostelium purpureum, no activity in Dictyostelium minutum, no activity in Polysphondylium pallidum, no activity in Polysphondylium violaceum PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 2.5.1.24)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)