Information on EC 2.4.1.247 - beta-D-galactosyl-(1->4)-L-rhamnose phosphorylase:
   PRINT
Please wait a moment until all data are loaded. This message will disappear when all data are loaded.
Mark a special word or phrase in this record:  
Select one or more organisms in this record:

The lowest common taxonomy group for this enzyme is: Clostridium phytofermentans

Show additional data Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

Please login to have access to the AMENDA and FRENDA data

EC NUMBERCOMMENTARY
2.4.1.247-

RECOMMENDED NAMEGeneOntology No.
beta-D-galactosyl-(1->4)-L-rhamnose phosphorylase-

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
beta-D-galactosyl-(1->4)-L-rhamnose + phosphate = L-rhamnose + alpha-D-galactose 1-phosphate
show the reaction diagram		----

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

PATHWAYKEGG LinkMetaCyc Link
No entries in this field

SYSTEMATIC NAMEIUBMB Comments
beta-D-galactosyl-(1->4)-L-rhamnose:phosphate 1-alpha-D-galactosyltransferaseThe enzyme from Clostridium phytofermentans is also active towards towards beta-D-galactosyl derivatives of L-mannose, L-lyxose, D-glucose, 2-deoxy-D-glucose, and D-galactose in this order. Differs from 1,3-beta-galactosyl-N-acetylhexosamine phosphorylase (EC 2.4.1.211) in being active towards L-rhamnose and inactive towards N-acetyl hexosamine derivatives.

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
cphy1920 proteinClostridium phytofermentansA9KHK4-693235
D-galactosyl-beta-1,4-L-rhamnose phosphorylaseClostridium phytofermentansA9KHK4-693235
GalRhaPClostridium phytofermentansA9KHK4-693235

CAS REGISTRY NUMBERCOMMENTARY
No entries in this field

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Clostridium phytofermentansbacterium possesses three GalHexNAcP homologous genes, cphy1920 gene; anaerobic cellulolytic bacterium693235A9KHK4UniProtManually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
2-deoxy-D-glucose + alpha-D-galactose 1-phosphateD-galactosyl-beta-1,3-2-deoxy-D-glucose + phosphate
show the reaction diagram		Clostridium phytofermentansA9KHK4when D-glucose derivatives are used as acceptors, reaction products are beta-1,3-galactosides693235--r
alpha-D-galactose 1-phosphate + L-rhamnoseD-galactosyl-beta-1,4-L-rhamnose + phosphate
show the reaction diagram		Clostridium phytofermentansA9KHK4highest activity693235--r
D-galactose + alpha-D-galactose 1-phosphateD-galactosyl-beta-1,3-D-galactose + phosphate
show the reaction diagram		Clostridium phytofermentansA9KHK4-693235--r
D-glucose + alpha-D-galactose 1-phosphateD-galactosyl-beta-1,3-D-glucose + phosphate
show the reaction diagram		Clostridium phytofermentansA9KHK4when D-glucose derivatives are used as acceptors, reaction products are beta-1,3-galactosides693235--r
L-lyxose + alpha-D-galactose 1-phosphateD-galactosyl-beta-1,4-L-lyxose + phosphate
show the reaction diagram		Clostridium phytofermentansA9KHK4-693235--r
L-mannose + alpha-D-galactose 1-phosphateD-galactosyl-beta-1,4-L-mannose + phosphate
show the reaction diagram		Clostridium phytofermentansA9KHK4-693235--r
additional information?-Clostridium phytofermentansA9KHK4phosphorylyzes neither galacto-N-biose (D-Gal-beta1,3-D-GalNAc) nor lacto-N-biose I(D-Gal-beta1,3-D-GlcNAc), thus indicating that Cphy1920 protein is not GalHexNAcP693235---

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
No entries in this field

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
No entries in this field

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
No entries in this field

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
290-2-deoxy-D-glucoseClostridium phytofermentansA9KHK4-693235 2D-image
610-D-galactoseClostridium phytofermentansA9KHK4-693235 2D-image
7.9-D-galactosyl-beta-1,4-L-rhamnoseClostridium phytofermentansA9KHK4-693235 2D-image
460-D-glucoseClostridium phytofermentansA9KHK4-693235 2D-image
220-L-LyxoseClostridium phytofermentansA9KHK4-693235 2D-image
78-L-MannoseClostridium phytofermentansA9KHK4-693235 2D-image
2.4-L-rhamnoseClostridium phytofermentansA9KHK4-693235 2D-image

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
282-deoxy-D-glucoseClostridium phytofermentansA9KHK4-693235 2D-image
24-D-galactoseClostridium phytofermentansA9KHK4-693235 2D-image
45-D-galactosyl-beta-1,4-L-rhamnoseClostridium phytofermentansA9KHK4-693235 2D-image
85-D-glucoseClostridium phytofermentansA9KHK4-693235 2D-image
120-L-LyxoseClostridium phytofermentansA9KHK4-693235 2D-image
170-L-MannoseClostridium phytofermentansA9KHK4-693235 2D-image
74-L-rhamnoseClostridium phytofermentansA9KHK4-693235 2D-image

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
67Clostridium phytofermentansA9KHK4optimum activity at neutral pH693235

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
No entries in this field

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
cytosolClostridium phytofermentansA9KHK4proteins are predicted not to possess N-terminal signal peptides5829693235Manually annotated by BRENDA team

PDBSCOPCATHORGANISM
No entries in this field

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
80000-Clostridium phytofermentansA9KHK4SDS-PAGE693235
84230-Clostridium phytofermentansA9KHK4calculated693235

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
37-Clostridium phytofermentansA9KHK4protein is thermostable up to 37°C693235

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-Clostridium phytofermentansA9KHK4693235

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
expressed in Escherichia coli BL21(DE3)Clostridium phytofermentansA9KHK4693235

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
synthesisClostridium phytofermentansA9KHK4suitable catalyst for practical syntheses of oligosaccharides693235

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISMLINK TO PUBMEDSOURCE
693235Nakajima, M.; Nishimoto, M.; Kitaoka, M.Characterization of three beta-galactoside phosphorylases from clostridium phytofermentans: discovery of D-galactosyl-beta1,4-L-rhamnose phosphorylaseJ. Biol. Chem.28419220-192272009Clostridium phytofermentans PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 2.4.1.247)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)