Information on EC 2.3.1.41 -

Information on EC 2.3.1.41 - beta-ketoacyl-acyl-carrier-protein synthase I:

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EC NUMBER	COMMENTARY
2.3.1.41	-

RECOMMENDED NAME	GeneOntology No.
beta-ketoacyl-acyl-carrier-protein synthase I	GO:0004315

REACTION	REACTION DIAGRAM	COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
an acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		-	-	-	-
an acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		mechanism	Escherichia coli	-	486863, 486864
an acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		structure	Escherichia coli	-	486916
an acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		acetylation of the synthase III-like polyketid synthase at Ser118	Streptomyces peucetius	-	486934
an acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		active site cysteine	Synechocystis sp.	-	486936
an acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		active site cysteine	Streptomyces glaucescens	-	486938
an acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		synthaseIII/FabH: His-Asn-Cys catalytic triad	Escherichia coli, Haemophilus influenzae	-	486939
an acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		synthaseIII/FabH: His-Asn-Cys catalytic triad	Streptococcus pneumoniae	P0A3C5	486939
an acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		active site cysteine; structure of active site, substrate binding pocket, and subunit structure in relation to substrate specificity of the 3 isozyme types	Synechocystis sp.	-	486940
an acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		synthaseII/FabB: His-HIs-Cys catalytic triad; synthaseIII/FabH: His-Asn-Cys catalytic triad	Escherichia coli	-	486941
an acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		mechanism and active site structure and function	Escherichia coli	-	486943
an acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		active site cysteine; mechanism	Rattus norvegicus	-	486944
an acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		active site cysteine	Coriandrum sativum	-	486948
an acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		mechanism, roles of H303, H337, C164	Streptococcus pneumoniae	-	659029
an acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		catalytic active site residues are Cys163 and His333, the active site contains two oxyanion holes, stabilizing the thioester oxyanion of acetylated CoA and of acetylated Cys92, respectively	Escherichia coli	P0A953	659726
an acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		comparison of active sites in isoforms KAS I, KAS II and related enzymes, geometry and catalytic role of equivalent residues	Escherichia coli	-	660470
an acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		beta-ketoacyl-acyl-carrier protein synthase enzyme joins short carbon units to construct fatty acyl chains by a three-step Claisen condensation reaction, the reaction starts with a trans thioesterification of the acyl primer substrate from ACP to the enzyme, subsequently, the donor substrate malonyl-ACP is decarboxylated to form a carbanion intermediate, which in the third step attacks C1 of the primer substrate giving rise to an elongated acyl chain, H298 serves as a catalytic base in the decarboxylation step, the enzyme possesses a Cys-His-His catalytic triad, Lys328 has a dual role in catalysis: its charge influences acyl transfer to the active site Cys, and the steric restraint imposed on H333, as an obligate hydrogen bond donor at Ne, is of critical importance for decarboxylation activity, active sites of the wild-type KAS I, its H298 mutants, and their acyl complexes, overview	Escherichia coli	-	673576
an acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein] = a 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		structure-activity analysis, reaction mechanism	Homo sapiens	Q9NWU1	677030

REACTION TYPE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
Acyl group transfer	-	-	-	-
condensation	Escherichia coli	-	-	486914, 486917, 486918, 486939, 486943
condensation	Brassica napus	-	-	486922
condensation	Streptomyces peucetius	-	-	486934
condensation	Synechocystis sp.	-	-	486936, 486940
condensation	Streptomyces glaucescens	-	-	486938
condensation	Streptococcus pneumoniae	P0A3C5	-	486939
condensation	Rattus norvegicus	-	-	486944
condensation	Staphylococcus aureus	P99159	-	486946
condensation	Cuphea lanceolata	-	-	486947
decarboxylation	Escherichia coli	-	-	486863, 486917, 486943
decarboxylation	Streptomyces glaucescens	-	-	486938
decarboxylation	Rattus norvegicus	-	-	486944
decarboxylation	Staphylococcus aureus	P99159	-	486946

PATHWAY	KEGG Link	MetaCyc Link
7-keto-8-aminopelargonate biosynthesis I	-	PWY-6519
fatty acid biosynthesis initiation II	-	PWY-5966
fatty acid biosynthesis initiation III	-	PWY-5965
fatty acid elongation -- saturated	-	FASYN-ELONG-PWY
mycolate biosynthesis	-	PWYG-321
palmitate biosynthesis I (animals)	-	PWY-5994
palmitate biosynthesis II (bacteria and plants)	-	PWY-5971
palmitoleate biosynthesis I	-	PWY-6282
stearate biosynthesis II (bacteria and plants)	-	PWY-5989
superpathway of fatty acid biosynthesis initiation (E. coli)	-	FASYN-INITIAL-PWY

SYSTEMATIC NAME

IUBMB Comments

acyl-[acyl-carrier protein]:malonyl-[acyl-carrier protein] C-acyltransferase (decarboxylating)

This enzyme is responsible for the chain-elongation step of dissociated (type II) fatty-acid biosynthesis, i.e. the addition of two C atoms to the fatty-acid chain. Escherichia coli mutants that lack this enzyme are deficient in unsaturated fatty acids. The enzyme can use fatty acyl thioesters of ACP (C₂ to C₁₆) as substrates, as well as fatty acyl thioesters of Co-A (C₄ to C₁₆) [4]. The substrate specificity is very similar to that of EC 2.3.1.179, beta-ketoacyl-ACP synthase II, with the exception that the latter enzyme is far more active with palmitoleoyl-ACP (C₁₆Delta9) as substrate, allowing the organism to regulate its fatty-acid composition with changes in temperature [4,5].

SYNONYMS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
3-ketoacyl-ACP synthase	Spinacia oleracea	-	-	486931
3-ketoacyl-ACP synthase	Escherichia coli	-	-	704553
3-ketoacyl-acyl carrier protein synthase	-	-	-	-
3-oxoacyl synthase	Rattus norvegicus	-	-	661000
3-oxoacyl-[acyl-carrier-protein] synthase	-	-	-	-
acyl-malonyl(acyl-carrier-protein)-condensing enzyme	-	-	-	-
beta-ketoacyl ACP synthase I	Escherichia coli	P0A953	-	659726
beta-ketoacyl acyl carrier protein synthase	-	-	-	-
beta-ketoacyl synthase	Rattus norvegicus	-	-	661000
beta-ketoacyl synthetase	-	-	-	-
beta-ketoacyl [ACP] synthase	Homo sapiens	Q9NWU1	-	677030
beta-ketoacyl-ACP synthase	Trypanosoma brucei	Q586W9	-	674882
beta-ketoacyl-ACP synthase	Homo sapiens	Q9NWU1	-	677030
beta-ketoacyl-ACP synthase I	Escherichia coli	-	-	673576
beta-ketoacyl-ACP synthetase	-	-	-	-
beta-ketoacyl-ACP-synthase	Arabidopsis thaliana	Q8L3X9	-	658746
beta-ketoacyl-acyl carrier protein synthetase	-	-	-	-
beta-ketoacyl-CoA synthase-I	Helianthus annuus	-	-	674095
beta-ketoacyl-[acyl carrier protein (ACP)] synthase III	Staphylococcus aureus	-	-	676903
beta-ketoacyl-[acyl carrier protein] synthase	-	-	-	-
beta-ketoacylsynthase	-	-	-	-
condensing enzyme	-	-	-	-
Cys-His-His-type beta-ketoacyl-acyl carrier protein synthase	Escherichia coli	-	-	673576
elongase	Trypanosoma brucei	Q586W9	-	674882
elongating beta-ketoacyl-acyl carrier protein synthase	Plasmodium falciparum	-	-	674777
FabB	Escherichia coli	-	-	704553
FabBF	Plasmodium falciparum	-	-	674777
FabH	Staphylococcus aureus	-	-	676903
fatty acid condensing enzyme	-	-	-	-
KAS	Spinacia oleracea	-	-	486931
KAS	Brassica napus	-	-	486932
KAS	Streptomyces glaucescens	-	-	486933, 486938
KAS	Allium ampeloprasum	-	-	486935
KAS	Synechocystis sp.	-	-	486936, 486940
KAS	Homo sapiens	Q9NWU1	-	677030
KAS I	Escherichia coli	-	-	659726, 673576
synthase, 3-oxoacyl-[acyl-carrier-protein]	-	-	-	-
KCSI	Helianthus annuus	-	-	674095
additional information	Escherichia coli	-	in vertebrates, yeast and Mycobacteria beta-ketoacyl synthase activity is a domain of the multifunctional polypeptide chains of fatty acid synthase EC 2.3.1.85 and EC 2.3.1.86	486863, 486864
additional information	Clostridium kluyveri	-	in vertebrates, yeast and Mycobacteria beta-ketoacyl synthase activity is a domain of the multifunctional polypeptide chains of fatty acid synthase EC 2.3.1.85 and EC 2.3.1.86	486863
additional information	Streptomyces glaucescens	-	enzyme shows also acetyl coenzyme A-acyl-carrier-protein transacylase activity EC 2.3.1.38, 12% compared to beta-keto-[acyl-carrier-protein] synthase activity EC 2.3.1.41	486933
additional information	Streptomyces peucetius	-	gene DpsC, encoding apolyketide synthase, exhibits also beta-keto-[acyl-carrier-protein] synthase III activity, which is restricted for propionyl-[acyl-carrier-protein] as the only [acyl-carrier-protein]-substrate	486934
additional information	Synechocystis sp.	-	3 structural classes of condensing enzymes	486940
additional information	Escherichia coli	P0A953	the enzyme belongs to the superfamily of condensing enzymes which includes thiolases	659726

CAS REGISTRY NUMBER	COMMENTARY
9077-10-5	-

ORGANISM	COMMENTARY	LITERATURE	SEQUENCE CODE	SEQUENCE DB	SOURCE
Allium ampeloprasum	synthase II	486935	-	-
Arabidopsis thaliana	-	658746	Q8L3X9	Swissprot
Arabidopsis thaliana	expression in Escherichia coli	659442	Q8L3X9	Swissprot
Bacillus subtilis	genes fabH1 and fabH2, 2 synthase III isozymes	486937	-	-
Brassica juncea	-	285675	-	-
Brassica napus	cv. Westar	486932	-	-
Brassica napus	synthase I and II	486922, 486923	-	-
Carthamus tinctorius	safflower	285669, 285675	-	-
Clostridium kluyveri	-	486863	-	-
Coriandrum sativum	seed plastid-specific isoform with similarities to synthase I type	486948	-	-
Cuphea lanceolata	synthase IV	486947	-	-
Cuphea lutea	-	285675	-	-
Escherichia coli	-	486863, 486864, 486914, 486915, 486917, 486918, 486939, 660470, 673576	-	-
Escherichia coli	-	659726	P0A953	Uniprot
Escherichia coli	B; synthases I and II	486919	-	-
Escherichia coli	gene fabB	704553	-	-
Escherichia coli	K12 strains UC1, WN1 is a fabF-mutant lacking synthase II, and various mutants, overview; synthases I and II	486916	-	-
Escherichia coli	synthase I	486943	-	-
Escherichia coli	synthase III, gene fabH	486932, 486937	-	-
Escherichia coli	synthases I, II, and III	486941	-	-
Euglena gracilis	Z	285676	-	-
Haemophilus influenzae	-	486939	-	-
Helianthus annuus	-	674095	-	-
Homo sapiens	expression in Escherichia coli, without N-terminal targeting sequence	659451	-	-
Homo sapiens	the human beta-ketoacyl [ACP] synthase from the mitochondrial type II fatty acid synthase	677030	Q9NWU1	SwissProt
Hordeum vulgare	cv. Bonus	486925	-	-
Lactococcus lactis	sp. lactis IL1403	659294	-	-
Mycobacterium tuberculosis	-	659493, 659724	-	-
Mycobacterium tuberculosis	synthase I, i.e. KasA	486942	P63454	SwissProt
Mycobacterium tuberculosis	synthase II, i.e. KasB	486942	-	-
Petroselinum crispum	-	486921	-	-
Pisum sativum	-	285675	-	-
Plasmodium falciparum	FabF/FabH; strain 3D7, gene fabBF, encoding the only elongating beta-ketoacyl-ACP synthase in the organism	674777	-	-
Rattus norvegicus	-	486944, 661000	-	-
Spinacia oleracea	-	486925	-	-
Spinacia oleracea	synthase I and II	285675, 486920, 486924	-	-
Spinacia oleracea	synthase III	486931	-	-
Staphylococcus aureus	-	660466	-	-
Staphylococcus aureus	methicillin-resistant and vancomycin-resistant strains	676903	-	-
Staphylococcus aureus	strain ATCC 35556; synthase III, gene fabH	486946	P99159	GenBank
Streptococcus pneumoniae	-	659029	-	-
Streptococcus pneumoniae	synthase III, gene fabH	486939	P0A3C5	SwissProt
Streptomyces glaucescens	synthase III, gene fabH	486933, 486938	-	-
Streptomyces peucetius	synthase III-like activity	486934	-	-
Synechocystis sp.	isozyme II	486940	-	-
Synechocystis sp.	synthase II	486936	-	-
Trypanosoma brucei	mitochondrial beta-ketoacyl synthase; ssp. brucei, strain 427, procyclic trypanosomes	674882	Q586W9	SwissProt

GENERAL INFORMATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
metabolism	Escherichia coli	-	FabB catalyzes the elongation of cis-3-decenoyl-ACP produced by FabA to generate the first 12-carbon cis-unsaturated fatty acid intermediate, 3-oxo-cis-5-decenoyl-ACP, which is converted to cis-5-dodecenoyl-ACP. FabB is essential in the unsaturated fatty acid, UFA, synthesis pathway, strains lacking FabB are UFA auxotroph, overview. FabB cannot be substituted by FabF	704553

SUBSTRATE	PRODUCT	REACTION DIAGRAM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY/ Substrate	LITERATURE/ Substrate	COMMENTARY/ Product	LITERATURE/ Product	Reversibility r=reversible ir=irreversible ?=not specified
4-hexadecenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxo-6-octadecenoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Coriandrum sativum	-	best substrate, highly specific for, reaction is part of petroselinic acid biosynthesis, synthase IV	486948	-	-	?
acetyl-CoA + malonyl-CoA	3-oxobutyryl-CoA + CoA		Rattus norvegicus	-	-	661000	formation of about 1% triacetic acid lactone	-	?
acetyl-CoA + malonyl-[acyl-carrier protein]	acetoacyl-[acyl-carrier protein] + CO2 + CoA		Haemophilus influenzae, Escherichia coli	-	-	486939	-	486939	?
acetyl-CoA + malonyl-[acyl-carrier protein]	acetoacyl-[acyl-carrier protein] + CO2 + CoA		Streptococcus pneumoniae	P0A3C5	synthase III, best substrate	486939	-	486939	?
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	-	486863, 486864	-	-	?
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	-	486914	-	486914	ir
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	-	486915	-	486915	r
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	-	486919	-	486919	r
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Spinacia oleracea	-	-	285675	-	-	?
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Petroselinum crispum	-	-	486921	-	486921	?
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Streptomyces glaucescens	-	synthase III	486933	-	-	?
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Staphylococcus aureus	P99159	synthase III	486946	-	486946	ir
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Spinacia oleracea	-	synthase III, recombinant enzyme from E. coli is absolutely specific for	486931	-	486931	r
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Spinacia oleracea	-	synthase I	486924	-	-	?
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	acetoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Mycobacterium tuberculosis	P63454	Kas A and KasB	486942	-	-	?
acetyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxobutanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Homo sapiens	-	-	659451	-	-	?
acyl-CoA + [acyl-carrier protein]	acyl-[acyl-carrier protein] + CoA		Escherichia coli	-	tetradecanoyl-CoA and cis-9-hexadecenoyl-CoA are poor substrates	486917	-	486917	r
acyl-CoA + [acyl-carrier protein]	acyl-[acyl-carrier protein] + CoA		Escherichia coli	-	catalyzes fatty acyl transfer between CoA and [acyl-carrier-protein], but no direct transfer to the enzyme as with acyl-[acyl-carrier-protein]	486863	-	-	?
acyl-CoA + [acyl-carrier protein]	acyl-[acyl-carrier protein] + CoA		Escherichia coli	-	catalyzes fatty acyl transfer between CoA and [acyl-carrier-protein], but no direct transfer to the enzyme as with acyl-[acyl-carrier-protein]	486917	-	486917	r
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	-	486937	-	-	?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Arabidopsis thaliana	Q8L3X9	-	659442	-	-	?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	part of non-associated fatty acid synthase system of plants and prokaryotes	486863, 486919	-	-	?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	part of non-associated fatty acid synthase system of plants and prokaryotes	486914	-	-	ir
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	part of non-associated fatty acid synthase system of plants and prokaryotes	486915	-	-	r
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Spinacia oleracea	-	part of non-associated fatty acid synthase system of plants and prokaryotes	486924	-	-	?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Clostridium kluyveri	-	part of non-associated fatty acid synthase system of plants and prokaryotes	486863	-	-	-
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	involved in biosynthesis of saturated fatty acids	486914	-	-	ir
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	involved in biosynthesis of saturated fatty acids	486915, 486917	-	-	r
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Spinacia oleracea	-	involved in biosynthesis of saturated fatty acids	486924	-	-	?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Brassica napus	-	involved in biosynthesis of saturated fatty acids	486922	-	-	r
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Cuphea lanceolata	-	key enzyme for regulation of medium-chain fatty acid synthesis in seeds	486947	-	-	?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Staphylococcus aureus	P99159	initiation of fatty acid biosynthesis, part of fatty acid synthase II	486946	-	-	ir
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli, Brassica napus	-	involved in biosynthesis of unsaturated and saturated fatty acids	486932	-	-	?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Mycobacterium tuberculosis	P63454	part of fatty acid synthase system I and II, involved in cell wall mycolic acids biosynthesis, overview	486942	-	-	?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Bacillus subtilis	-	initiation of fatty acid biosynthesis with acetyl-CoA as a primer, overview substrate specificity	486937	-	-	?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	responsible for chain elongation during de novo fatty acid synthesis	486863	-	-	?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Clostridium kluyveri	-	responsible for chain elongation during de novo fatty acid synthesis	486863	-	-	-
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	involved in biosynthesis of mono-unsaturated long-chain fatty acids	486919	-	-	?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Streptomyces glaucescens	-	initiation of both straight- and branched-chain fatty acid biosynthesis resulting in a wide variety of produced fatty acids in vivo	486933, 486938	-	-	?
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	acyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Plasmodium falciparum	-	C4 to C14 acyl-[ACP]s, the enzyme readily elongates C4:0-through C10:0-PfACPs, has significantly less activity with C12:0- and C14:0-ACPs, and no capacity for elongation of C16:0-ACP and C18:0-ACP	674777	-	-	?
an acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	a 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Helianthus annuus	-	the endoplasmic reticulum enzyme is involved in synthesis of very long chain fatty acids, KCS-I displays a preference for 20:0-CoA, substrate specificity, overview	674095	-	-	?
butanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxohexanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Homo sapiens	-	-	659451	-	-	?
butyryl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	beta-ketohexanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	-	486914	-	-	ir
butyryl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	beta-ketohexanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Streptomyces glaucescens	-	synthase III	486933	-	-	?
butyryl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	beta-ketohexanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Staphylococcus aureus	P99159	synthase III	486946	-	-	?
C10-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	C12-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Plasmodium falciparum	-	-	674777	-	-	?
C12-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	C14-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Plasmodium falciparum	-	-	674777	-	-	?
C14-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	C16-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Plasmodium falciparum	-	-	674777	-	-	?
C4-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	C6-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Plasmodium falciparum	-	-	674777	-	-	?
C6-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	C8-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Plasmodium falciparum	-	best substrate	674777	-	-	?
C8-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	C10-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Plasmodium falciparum	-	-	674777	-	-	?
cis-3-decenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxo-cis-5-dodecenoyl-[acyl-carrier protein] + acyl-carrier protein		Escherichia coli	-	-	486919	-	-	-
cis-3-decenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxo-cis-5-dodecenoyl-[acyl-carrier protein] + acyl-carrier protein		Escherichia coli	-	-	704553	-	-	?
cis-3-decenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxo-cis-5-dodecenoyl-[acyl-carrier protein] + acyl-carrier protein		Escherichia coli	-	key step in the unsaturated fatty acid, UFA, synthesis pathway. FabB is negatively regulated by the FabR protein	704553	the product is not incorporated into the complex lipids of the bacterium	-	?
cis-9-hexadecenoyl-CoA + malonyl-[acyl-carrier protein]	3-oxo-hexadecenoyl-[acyl-carrier protein] + CO2 + CoA		Escherichia coli	-	-	486919	-	-	?
cis-9-hexadecenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxo-cis-11-octadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	-	486863	-	-	?
cis-9-hexadecenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxo-cis-11-octadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	-	486917	-	-	r
cis-9-hexadecenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxo-cis-11-octadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	poor substrate, synthase I	486916	-	-	?
cis-9-hexadecenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxo-cis-11-octadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	poor substrate, synthase I	486919	-	-	r
cis-9-hexadecenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxo-cis-11-octadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	best substrate, synthase II	486916	-	-	?
cis-9-hexadecenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxo-cis-11-octadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	best substrate, synthase II	486919	-	-	r
cis-mono-unsaturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	cis-mono-unsaturated 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	-	486864	-	-	?
cis-mono-unsaturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	cis-mono-unsaturated 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Clostridium kluyveri	-	-	486863	-	-	?
cis-mono-unsaturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	cis-mono-unsaturated 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	not cis-vaccenoyl-[acyl-carrier-protein], i.e. cis-11-octenoyl-[acyl-carrier-protein]	486863, 486916	-	-	?
cis-mono-unsaturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	cis-mono-unsaturated 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	not cis-vaccenoyl-[acyl-carrier-protein], i.e. cis-11-octenoyl-[acyl-carrier-protein]	486917	-	-	r
cis-mono-unsaturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	cis-mono-unsaturated 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	from C-10 to C-16, e.g. cis-5-decenoyl-[acyl-carrier-protein], cis-5-dodecenoyl-[acyl-carrier-protein], cis-7-tetradecenoyl-[acyl-carrier-protein], cis-9-hexenoyl-[acyl-carrier-protein], i.e. palmitoleoyl-[acyl-carrier-protein], poor substrates	486919	-	-	-
cis-mono-unsaturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	cis-mono-unsaturated 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	from C-10 to C-16, e.g. cis-5-decenoyl-[acyl-carrier-protein], cis-5-dodecenoyl-[acyl-carrier-protein], cis-7-tetradecenoyl-[acyl-carrier-protein], cis-9-hexenoyl-[acyl-carrier-protein], i.e. palmitoleoyl-[acyl-carrier-protein], poor substrates	486916	-	-	?
cis-mono-unsaturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	cis-mono-unsaturated 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	from C-10 to C-16, e.g. cis-5-decenoyl-[acyl-carrier-protein], cis-5-dodecenoyl-[acyl-carrier-protein], cis-7-tetradecenoyl-[acyl-carrier-protein], cis-9-hexenoyl-[acyl-carrier-protein], i.e. palmitoleoyl-[acyl-carrier-protein], poor substrates	486917	-	-	r
decanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	dodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	-	486863	-	-	?
decanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	dodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Homo sapiens	-	-	659451	-	-	?
decanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	dodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Spinacia oleracea	-	-	486920	-	-	?
decanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	dodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Mycobacterium tuberculosis	-	-	659493	-	-	?
decanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	dodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Spinacia oleracea	-	synthase I	486924	-	-	?
decanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	dodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Cuphea lanceolata	-	synthase IV	486947	-	-	?
dodecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxotetradecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	-	486863, 486916	-	-	?
dodecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxotetradecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Homo sapiens	-	-	659451	-	-	?
dodecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxotetradecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Spinacia oleracea	-	-	285675	-	-	?
dodecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxotetradecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Mycobacterium tuberculosis	-	-	659493	-	-	?
eicosanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxodocosanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Mycobacterium tuberculosis	-	-	659493	-	-	?
eicosanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxodocosanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Mycobacterium tuberculosis	P63454	Kas A and KasB	486942	-	-	?
hexadecanoyl-CoA + malonyl-[acyl-carrier protein]	3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + CoA		Mycobacterium tuberculosis	P63454	Kas A and KasB, preference for	486942	-	-	?
hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Coriandrum sativum	-	-	486948	-	-	?
hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	not	486863, 486864	-	-	-
hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Spinacia oleracea	-	poor substrate	486920	-	-	?
hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Mycobacterium tuberculosis	P63454	KasA and KasB, preference for	486942	-	-	?
hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Spinacia oleracea	-	only synthase II, best substrate	486924	-	-	?
hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	i.e. palmitoyl-[acyl-carrier-protein]	486916	-	-	?
hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Spinacia oleracea	-	i.e. palmitoyl-[acyl-carrier-protein]	486920, 486924	-	-	?
hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Brassica napus	-	i.e. palmitoyl-[acyl-carrier-protein]	486923	-	-	?
hexadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Spinacia oleracea	-	preferred substrate, synthase III	285675	-	-	?
hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	-	486914	-	-	ir
hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	-	486917	-	-	r
hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Homo sapiens	-	-	659451	-	-	?
hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Spinacia oleracea	-	-	285675	-	-	?
hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Brassica napus	-	-	486923	-	-	?
hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Mycobacterium tuberculosis	-	-	659493	-	-	?
hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Synechocystis sp.	-	-	486936	-	-	?
hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	characterization of hexanoyl-enzyme intermediate	486918	-	-	r
hexanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Spinacia oleracea	-	best substrate, synthase I	486920, 486924	-	-	?
isobutyryl-CoA + malonyl-[acyl-carrier protein]	?		Streptomyces glaucescens	-	synthase III	486933, 486938	-	-	?
isobutyryl-CoA + malonyl-[acyl-carrier protein]	?		Staphylococcus aureus	P99159	synthase III	486946	-	-	?
isobutyryl-CoA + malonyl-[acyl-carrier protein]	?		Streptomyces glaucescens	-	isobutyryl-CoA is a precursor of branched-chain fatty acid synthesis	486938	-	-	?
Malonyl-CoA	Acetyl-CoA + CO2		Escherichia coli	-	decarboxylation reaction	673576	-	-	?
malonyl-[acyl-carrier protein]	acetyl-[acyl-carrier protein] + CO2		Streptomyces glaucescens	-	-	486938	-	-	?
malonyl-[acyl-carrier protein]	acetyl-[acyl-carrier protein] + CO2		Escherichia coli	-	catalyzes malonyl-[acyl-carrier-protein] decarboxylation independent of fatty acyl-[acyl-carrier-protein]	486917	-	486917	?
malonyl-[acyl-carrier protein]	acetyl-[acyl-carrier protein] + CO2		Escherichia coli	-	decarboxylation with 2-3% the rate of beta-ketoacyl-[acyl-carrier-protein]-synthesis	486863	-	486863	?
malonyl-[acyl-carrier protein]	acetyl-[acyl-carrier protein] + CO2		Escherichia coli	-	wild-type synthase I and mutants C163A and C163S	486943	-	486943	?
malonyl-[acyl-carrier protein] + lauroyl-CoA	? + CO2 + [acyl-carrier protein]		Mycobacterium tuberculosis	-	-	659724	-	-	?
malonyl-[acyl-carrier protein] + myristoyl-CoA	? + CO2 + [acyl-carrier protein]		Mycobacterium tuberculosis	-	-	659724	-	-	?
myristoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Homo sapiens	-	-	659451	-	-	?
myristoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Mycobacterium tuberculosis	-	-	659493	-	-	?
octadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoeicosanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Mycobacterium tuberculosis	-	-	659493	-	-	?
octadecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoeicosanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Brassica napus	-	synthase I and II	486923	-	-	?
octanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	-	486914	-	-	ir
octanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	-	486918	-	486918	r
octanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Homo sapiens	-	-	659451	-	-	?
octanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Mycobacterium tuberculosis	-	-	659493	-	-	?
octanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxodecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Synechocystis sp.	-	-	486936	-	-	?
palmitoyl-[acyl-carrier protein] + malonyl-CoA	3-oxostearoyl-[acyl-carrier protein] + CO2 + CoA		Allium ampeloprasum	-	-	486935	-	-	?
palmitoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxooctadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Mycobacterium tuberculosis	-	-	659493	-	-	?
palmitoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxooctanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Homo sapiens	-	-	659451	-	-	?
propionyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	beta-ketopentanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	-	486914	-	-	ir
propionyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	beta-ketopentanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Streptomyces peucetius	-	absolutely specific for	486934	-	-	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	-	486941	-	486941	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	-	486943	-	486943	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	-	486915	-	486915	r
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	-	486918	-	486918	r
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Rattus norvegicus	-	-	486944	-	486944	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Euglena gracilis	-	-	285676	-	285676	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Hordeum vulgare	-	-	486925	-	486925	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Spinacia oleracea	-	-	285675	-	285675	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Spinacia oleracea	-	-	486925	-	486925	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Spinacia oleracea	-	-	486931	-	486931	r
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Pisum sativum	-	-	285675	-	285675	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Petroselinum crispum	-	-	486921	-	486921	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Brassica napus	-	-	486923	-	486923	-
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Brassica napus	-	-	486922	-	486922	r
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Clostridium kluyveri	-	-	486863	-	486863	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Synechocystis sp.	-	-	486936	-	486936	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Synechocystis sp.	-	-	486940	-	486940	r
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Brassica juncea	-	-	285675	-	285675	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Carthamus tinctorius	-	-	285669	-	285669	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Carthamus tinctorius, Cuphea lutea	-	-	285675	-	285675	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Staphylococcus aureus	P99159	-	486946	-	486946	ir
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Allium ampeloprasum	-	-	486935	synthase II, parenchyma and lamina epidermis: palmitoyl-[acyl-carrier-protein] is the only product, sheath epidermis: acyl-[acyl-carrier-protein] up to C22 chain length	486935	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Haemophilus influenzae	-	substrate specificity, overview	486939	-	486939	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Bacillus subtilis	-	substrate specificity, overview	486937	-	486937	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	substrate specificity, overview	486916	-	486916	-
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	substrate specificity, overview	486863	-	486863	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	substrate specificity, overview	486937	-	486937	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	substrate specificity, overview	486939	-	486939	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	substrate specificity, overview	486914	-	486914	ir
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	substrate specificity, overview	486917	-	486917	r
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	substrate specificity, overview	486919	-	486919	r
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Spinacia oleracea	-	substrate specificity, overview	486920	-	486920	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Spinacia oleracea	-	substrate specificity, overview	486924	-	486924	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Mycobacterium tuberculosis	P63454	substrate specificity, overview	486942	-	486942	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Streptomyces glaucescens	-	substrate specificity, overview	486933	-	486933	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Streptomyces glaucescens	-	substrate specificity, overview	486938	-	486938	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Cuphea lanceolata	-	substrate specificity, overview	486947	-	486947	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Streptococcus pneumoniae	P0A3C5	substrate specificity, overview	486939	-	486939	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Cuphea lanceolata	-	preference for medium-chain length [acyl-carrier-protein]-substrates, synthase IV	486947	-	486947	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Streptomyces glaucescens	-	synthase III, broad specificity	486933	-	486933	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Streptomyces glaucescens	-	synthase III, broad specificity	486938	-	486938	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	not hexadecanoyl-[acyl-carrier-protein]	486863	-	486863	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	not hexadecanoyl-[acyl-carrier-protein]	486864	-	486864	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	specific for acyl-[acyl-carrier-protein] thioesters, not acyl-CoA or acyl pantetheine thioesters	486863	-	486863	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	specific for acyl-[acyl-carrier-protein] thioesters, not acyl-CoA or acyl pantetheine thioesters	486864	-	486864	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli, Brassica napus	-	fatty acid composition of Brassica napus wild-type and transgenic plants overexpressing the E. coli synthase III	486932	-	486932	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Mycobacterium tuberculosis	P63454	KasA and KasB, preference for long-chain acyl-[acyl-carrier-protein]s with at least 16 C-atoms	486942	-	486942	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Spinacia oleracea	-	not octodecanoyl-[acyl-carrier-protein], i.e stearoyl-[acyl-carrier-protein]	486920	-	486920	?
saturated acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Spinacia oleracea	-	not octodecanoyl-[acyl-carrier-protein], i.e stearoyl-[acyl-carrier-protein]	486924	-	486924	?
stearoyl-[acyl-carrier protein] + malonyl-CoA	3-oxoeicosanoyl-[acyl-carrier protein] + CO2 + CoA		Allium ampeloprasum	-	only in sheath epidermis	486935	-	-	?
tetradecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	-	486863, 486916	-	-	?
tetradecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Spinacia oleracea	-	-	486920	-	-	-
tetradecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Spinacia oleracea	-	-	285675	-	-	?
tetradecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	poor substrate	486917	-	-	r
tetradecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	synthase I	486943	-	-	?
tetradecanoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxohexadecanoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Spinacia oleracea	-	i.e. myristoyl-[acyl-carrier-protein], only synthase II	486924	-	-	?
malonyl-[acyl-carrier protein] + palmitoyl-CoA	? + CO2 + [acyl-carrier protein]		Mycobacterium tuberculosis	-	-	659724	-	-	?
additional information	?	-	Escherichia coli	-	-	486937, 486939	-	-	-
additional information	?	-	Escherichia coli	-	enzyme also catalyzes a malonyl-CoA-CO2 exchange reaction, overview: substrates and kinetics	486917	-	-	-
additional information	?	-	Streptococcus pneumoniae	P0A3C5	synthase III, FabH, has a preference for straight short-chain CoA primers	486939	-	-	-
additional information	?	-	Escherichia coli	-	synthase I and II have nearly the same substrate specificity	486919	-	-	-
additional information	?	-	Spinacia oleracea	-	synthase I is specific for short-chain fatty acid prolongation while synthase II is active in C18 formation from C16	486924	-	-	-
additional information	?	-	Bacillus subtilis	-	synthase III isozymes also utilize iso- and anteiso-branched-chain acyl-CoA primers as substrates	486937	-	-	-
additional information	?	-	Streptomyces glaucescens	-	malonyl-CoA-CO2 exchange reaction	486933	-	-	-
additional information	?	-	Homo sapiens	-	important role in generation of the lipoic acid precursor octanoyl-acyl-carrier protein	659451	-	-	-
additional information	?	-	Mycobacterium tuberculosis	-	enzyme is able to use Escherichia coli acyl-carrier protein. Enzyme extends longer, physiologically relevant acyl-CoA primers when paired with its native acyl-carrier protein than with Escherichia coli acyl-carrier protein	659493	-	-	-
additional information	?	-	Arabidopsis thaliana	Q8L3X9	no substrate: acetyl-[acyl-carrier protein]	659442	-	-	-
additional information	?	-	Homo sapiens	-	preference for acyl-[acyl-carrier protein] in decreasing order: C12, C10, C6, C8, C4, C2, C14, C16	659451	-	-	-
additional information	?	-	Staphylococcus aureus	-	rank of activity in decreasing order: isobutyryl-CoA, hexanoyl-CoA, butyryl-CoA, isovaleryl-CoA, acetyl-CoA	660466	-	-	-
additional information	?	-	Staphylococcus aureus	-	inhibition of FabH leads to loss of accumulation of acetoacetyl-ACP	676903	-	-	-
additional information	?	-	Trypanosoma brucei	Q586W9	the organism, with the unusual lipid metabolism of the trypanosome, uses a microsomal elongase pathway, ELO, for de novo fatty acid synthesis, a second FAS pathway with a type II synthase is localized to the mitochondrion and is essential for bloodstream and procyclic life cycle stages of the parasite, in vivo precursors of mitochondrial fatty acid synthesis, overview	674882	-	-	-
additional information	?	-	Escherichia coli	-	enzyme active site structure of wild-type and mutants with or without bound C12 or C8, overview	673576	-	-	-
additional information	?	-	Staphylococcus aureus	-	FabH produces C14 long-chain beta-ketoacyl-ACP	676903	-	-	-
additional information	?	-	Homo sapiens	Q9NWU1	KAS catalyzes the C2 fatty acid elongation reaction using a Cys-His-His catalytic triad	677030	-	-	-
additional information	?	-	Trypanosoma brucei	Q586W9	octanoate, the precursor for lipoic acid synthesis, is also produced by the mitochondrial FAS	674882	-	-	-

NATURAL SUBSTRATES	NATURAL PRODUCTS	REACTION DIAGRAM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY SUBSTRATE	LITERATURE (Substrate)	COMMENTARY PRODUCT	LITERATURE (Product)
4-hexadecenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxo-6-octadecenoyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Coriandrum sativum	-	reaction is part of petroselinic acid biosynthesis, synthase IV	486948	-	-
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	-	486937	-	-
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	part of non-associated fatty acid synthase system of plants and prokaryotes	486863, 486919, 486914, 486915	-	-
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Spinacia oleracea	-	part of non-associated fatty acid synthase system of plants and prokaryotes	486924	-	-
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Clostridium kluyveri	-	part of non-associated fatty acid synthase system of plants and prokaryotes	486863	-	-
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	involved in biosynthesis of saturated fatty acids	486914, 486915, 486917	-	-
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Spinacia oleracea	-	involved in biosynthesis of saturated fatty acids	486924	-	-
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Brassica napus	-	involved in biosynthesis of saturated fatty acids	486922	-	-
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Cuphea lanceolata	-	key enzyme for regulation of medium-chain fatty acid synthesis in seeds	486947	-	-
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Staphylococcus aureus	P99159	initiation of fatty acid biosynthesis, part of fatty acid synthase II	486946	-	-
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli, Brassica napus	-	involved in biosynthesis of unsaturated and saturated fatty acids	486932	-	-
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Mycobacterium tuberculosis	P63454	part of fatty acid synthase system I and II, involved in cell wall mycolic acids biosynthesis, overview	486942	-	-
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Bacillus subtilis	-	initiation of fatty acid biosynthesis with acetyl-CoA as a primer, overview substrate specificity	486937	-	-
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli, Clostridium kluyveri	-	responsible for chain elongation during de novo fatty acid synthesis	486863	-	-
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Escherichia coli	-	involved in biosynthesis of mono-unsaturated long-chain fatty acids	486919	-	-
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Streptomyces glaucescens	-	initiation of both straight- and branched-chain fatty acid biosynthesis resulting in a wide variety of produced fatty acids in vivo	486933, 486938	-	-
acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	acyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Plasmodium falciparum	-	-	674777	-	-
an acyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	a 3-oxoacyl-[acyl-carrier protein] + CO2 + [acyl-carrier protein]		Helianthus annuus	-	the endoplasmic reticulum enzyme is involved in synthesis of very long chain fatty acids	674095	-	-
cis-3-decenoyl-[acyl-carrier protein] + malonyl-[acyl-carrier protein]	3-oxo-cis-5-dodecenoyl-[acyl-carrier protein] + acyl-carrier protein		Escherichia coli	-	key step in the unsaturated fatty acid, UFA, synthesis pathway. FabB is negatively regulated by the FabR protein	704553	the product is not incorporated into the complex lipids of the bacterium	-
additional information	?	-	Homo sapiens	-	important role in generation of the lipoic acid precursor octanoyl-acyl-carrier protein	659451	-	-
additional information	?	-	Staphylococcus aureus	-	inhibition of FabH leads to loss of accumulation of acetoacetyl-ACP	676903	-	-
additional information	?	-	Trypanosoma brucei	Q586W9	the organism, with the unusual lipid metabolism of the trypanosome, uses a microsomal elongase pathway, ELO, for de novo fatty acid synthesis, a second FAS pathway with a type II synthase is localized to the mitochondrion and is essential for bloodstream and procyclic life cycle stages of the parasite, in vivo precursors of mitochondrial fatty acid synthesis, overview	674882	-	-

COFACTOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

METALS and IONS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
Mg2+	Streptococcus pneumoniae	-	-	659029

INHIBITORS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
4-phenyl-5-phenylimino-[1,2,4]dithiazolidin-3-one	Staphylococcus aureus	P99159	i.e. HR19; strong, synthase III	486946	2D-image
5-chloro-4-phenyl-[1,2]-dithiol-3-one	Staphylococcus aureus	P99159	complete inhibition at 0.01 nM, synthase III; i.e. HR12	486946	2D-image
acetyl-CoA	Escherichia coli	-	-	486914	2D-image
acyl-CoA	Escherichia coli	-	at high concentrations	486914	2D-image
arsenite	Spinacia oleracea	-	-	285675	2D-image
arsenite	Spinacia oleracea	-	weak at 1 mM	486920	2D-image
arsenite	Spinacia oleracea	-	synthase II and I	486924	2D-image
arsenite	Spinacia oleracea	-	synthase III	486931	2D-image
Butyryl-CoA	Streptomyces glaucescens	-	synthase III mutants C122Aand C122Q: inhibition of decarboxylation activity in presence of	486938	2D-image
C75	Rattus norvegicus	-	a synthetic inhibitor of fatty acid synthase, inhibits also the beta-ketoacyl synthase, not in simple first order rate reaction, C75 is a competitive, irreversible inhibitor of the overall reaction, the beta-ketoacyl synthase activity and the enoyl reductase activity substrates do not protect the thioesterase activity of rat liver FAS from inactivation by C75, but malonyl-CoA and acetyl-CoA protect the FAS beta-ketoacyl synthase reaction from inactivation by C75 in a competitive manner, overview	661000	2D-image
cerulenin	Carthamus tinctorius	-	-	285669	2D-image
cerulenin	Spinacia oleracea	-	antibiotic, protection by hexanoyl-[acyl-carrier-protein], decanoyl-[acyl-carrier-protein], and tetradecanoyl-[acyl-carrier-protein]	486920	2D-image
cerulenin	Brassica napus	-	no inhibition	486923	2D-image
cerulenin	Spinacia oleracea	-	-	486924	2D-image
cerulenin	Allium ampeloprasum	-	synthase II, moderate	486935	2D-image
cerulenin	Synechocystis sp.	-	12-carbon mycotoxin, irreversible inhibition, binding motif; at 0.05 mM: wild-type is completely inactivated, mutant I108F and A193M show only 10% reduced activity	486936	2D-image
cerulenin	Escherichia coli, Haemophilus influenzae	-	-	486939	2D-image
cerulenin	Streptococcus pneumoniae	P0A3C5	-	486939	2D-image
cerulenin	Escherichia coli	-	analysis of the binding mechanism, mimicks the condensation transition state; FabB: inhibition is irreversible	486941	2D-image
cerulenin	Mycobacterium tuberculosis	P63454	Kas A and KasB	486942	2D-image
cerulenin	Staphylococcus aureus	P99159	weak, synthase III	486946	2D-image
cerulenin	Cuphea lanceolata	-	synthase IV	486947	2D-image
cerulenin	Coriandrum sativum	-	strong	486948	2D-image
cerulenin	Arabidopsis thaliana	-	-	658746, 659442	2D-image
cerulenin	Homo sapiens	-	50% inhibition at 0.2 mM	659451	2D-image
cerulenin	Rattus norvegicus	-	specific inhibition of the enzyme	661000	2D-image
cerulenin	Plasmodium falciparum	-	irreversible inhibition, IC50: 0.0158 mM	674777	2D-image
cerulenin	Homo sapiens	Q9NWU1	-	677030	2D-image
iodoacetamide	Escherichia coli	-	no inhibition of malonyl-[acyl-carrier-protein]-decarboxylation	486863	2D-image
iodoacetamide	Escherichia coli	-	-	486864	2D-image
iodoacetamide	Escherichia coli	-	acyl-[acyl-carrier-protein]-derivatives, e.g. acetyl-[acyl-carrier-protein], hexanoyl-CoA, octanoyl-CoA, decanoyl-CoA protect	486914, 486917	2D-image
iodoacetamide	Escherichia coli	-	after incubation with 2-mercaptethanol	486915	2D-image
iodoacetamide	Escherichia coli	-	acyl-[acyl-carrier-protein]-derivatives, e.g. acetyl-[acyl-carrier-protein], hexanoyl-CoA, octanoyl-CoA, decanoyl-CoA protect; synthase I and II	486919	2D-image
iodoacetamide	Rattus norvegicus	-	kinetics	486944	2D-image
N-ethylmaleimide	Clostridium kluyveri, Escherichia coli	-	no inhibition of malonyl-[acyl-carrier-protein]-decarboxylation	486863	2D-image
N-ethylmaleimide	Escherichia coli	-	-	486864	2D-image
N-ethylmaleimide	Escherichia coli	-	acyl-[acyl-carrier-protein]-derivatives protect	486914	2D-image
N-ethylmaleimide	Escherichia coli	-	after incubation with 2-mercaptoethanol	486915	2D-image
N-ethylmaleimide	Spinacia oleracea	-	5 mM	486920, 486924	2D-image
N-ethylmaleimide	Spinacia oleracea	-	-	486931	2D-image
p-chloromercuribenzoate	Spinacia oleracea	-	complete inhibition at 1 mM	486920, 486924	2D-image
platencin	Staphylococcus aureus	-	exhibits a broad-spectrum Gram-positive antibacterial activity through inhibition of fatty acid biosynthesis, targets the two essential proteins, beta-ketoacyl-[acyl carrier protein] synthase II and III, i.e. FabF and FabH, purified FabH IC50: 0.016 mM, overview	676903	2D-image
SB418011	Escherichia coli, Haemophilus influenzae	-	-	486939	2D-image
SB418011	Streptococcus pneumoniae	P0A3C5	strong, synthase III	486939	2D-image
thiolactomycin	Streptomyces glaucescens	-	synthase III	486933	2D-image
thiolactomycin	Escherichia coli	-	synthase III	486939	2D-image
thiolactomycin	Haemophilus influenzae	-	-	486939	2D-image
thiolactomycin	Streptococcus pneumoniae	P0A3C5	synthase III; weak	486939	2D-image
thiolactomycin	Escherichia coli	-	analysis of the binding mechanism, mimics malonyl-[acyl-carrier-protein]; FabB: inhibition is reversible, competitive	486941	2D-image
thiolactomycin	Mycobacterium tuberculosis	P63454	Kas A and KasB	486942	2D-image
thiolactomycin	Staphylococcus aureus	P99159	synthase III; weak	486946	2D-image
thiolactomycin	Plasmodium falciparum	-	competitive inhibition, IC50: 0.0236 mM	674777	2D-image
Urea	Escherichia coli	-	about 50% inhibition at 1 M, complete inactivation at 4 M	486918	2D-image
[Acyl-carrier-protein]	Escherichia coli	-	wild-type synthase I and mutants, not mutants C163S and K328A, overview	486943	2D-image
isobutyryl-CoA	Streptomyces glaucescens	-	synthase III mutants C122Aand C122Q: inhibition of decarboxylation activity in presence of	486938	2D-image
additional information	Helianthus annuus	-	the enzyme shows strong substrate inhibition	674095	-
additional information	Staphylococcus aureus	-	in vivo inhibition assays, no inhibition by platensimycin	676903	-
additional information	Homo sapiens	Q9NWU1	inhibitor binding structure determination	677030	-

ACTIVATING COMPOUND	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
2-mercaptoethanol	Escherichia coli	-	activation	486863, 486864, 486914	2D-image
2-mercaptoethanol	Escherichia coli	-	required in high concentration, 0.5 M	486915	2D-image
2-mercaptoethanol	Escherichia coli	-	required	486918	2D-image
dithiothreitol	Escherichia coli	-	activation	486863	2D-image
dithiothreitol	Escherichia coli	-	activation; can replace 2-mercaptoethanol	486915	2D-image
dithiothreitol	Escherichia coli	-	activation; required	486916	2D-image
dithiothreitol	Escherichia coli	-	required	486918	2D-image
EDTA	Escherichia coli	-	activation	486863, 486916	2D-image
EDTA	Escherichia coli	-	7 mM, stimulates	486915	2D-image
phosphate	Escherichia coli	-	activation, 0.2 M	486864	2D-image

KM VALUE [mM]	KM VALUE [mM] Maximum	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
0.0024	-	acetyl-CoA	Streptomyces glaucescens	-	synthase III	486933	2D-image
0.0062	-	acetyl-CoA	Staphylococcus aureus	P99159	synthase III	486946	2D-image
0.0403	-	acetyl-CoA	Streptococcus pneumoniae	P0A3C5	synthase III	486939	2D-image
0.019	-	acetyl-[acyl-carrier protein]	Escherichia coli	-	pH 7.0	486915	2D-image
0.031	-	acetyl-[acyl-carrier protein]	Escherichia coli	-	synthase I	486919	2D-image
0.04	-	acetyl-[acyl-carrier protein]	Escherichia coli	-	acyl-carrier-protein, fatty acyl transfer between tetradecanoyl-CoA and acyl-carrier-protein	486917	2D-image
0.04	-	acetyl-[acyl-carrier protein]	Escherichia coli	-	synthase II	486919	2D-image
0.05	-	acetyl-[acyl-carrier protein]	Homo sapiens	-	pH 6.8, 37�C	659451	2D-image
0.0039	-	butanoyl-[acyl-carrier protein]	Homo sapiens	-	pH 6.8, 37�C	659451	2D-image
0.00071	-	Butyryl-CoA	Streptomyces glaucescens	-	synthase III	486933	2D-image
0.0023	-	Butyryl-CoA	Staphylococcus aureus	P99159	synthase III	486946	2D-image
0.403	-	C10-[acyl-carrier protein]	Plasmodium falciparum	-	pH 7.5, 37�C, recombinant enzyme	674777	2D-image
0.196	-	C12-[acyl-carrier protein]	Plasmodium falciparum	-	pH 7.5, 37�C, recombinant enzyme	674777	2D-image
0.437	-	C14-[acyl-carrier protein]	Plasmodium falciparum	-	pH 7.5, 37�C, recombinant enzyme	674777	2D-image
0.211	-	C4-[acyl-carrier protein]	Plasmodium falciparum	-	pH 7.5, 37�C, recombinant enzyme	674777	2D-image
0.116	-	C6-[acyl-carrier protein]	Plasmodium falciparum	-	pH 7.5, 37�C, recombinant enzyme	674777	2D-image
0.198	-	C8-[acyl-carrier protein]	Plasmodium falciparum	-	pH 7.5, 37�C, recombinant enzyme	674777	2D-image
0.012	-	cis-3-decenoyl-[acyl-carrier protein]	Escherichia coli	-	synthase I	486919	2D-image
0.014	-	cis-3-decenoyl-[acyl-carrier protein]	Escherichia coli	-	synthase II	486919	2D-image
0.017	-	cis-9-hexadecenoyl-[acyl-carrier protein]	Escherichia coli	-	synthase II	486919	2D-image
0.138	-	cis-9-hexadecenoyl-[acyl-carrier protein]	Escherichia coli	-	synthase I	486919	2D-image
0.0018	-	decanoyl-[acyl-carrier protein]	Homo sapiens	-	pH 6.8, 37�C	659451	2D-image
0.0031	-	decanoyl-[acyl-carrier protein]	Cuphea lanceolata	-	synthase IV	486947	2D-image
0.0133	-	decanoyl-[acyl-carrier protein]	Spinacia oleracea	-	synthase II	486924	2D-image
0.0023	-	dodecanoyl-[acyl-carrier protein]	Mycobacterium tuberculosis	-	pH 7.0, 37�C	659493	2D-image
0.0095	-	dodecanoyl-[acyl-carrier protein]	Homo sapiens	-	pH 6.8, 37�C	659451	2D-image
0.002	-	eicosanoyl-[acyl-carrier protein]	Mycobacterium tuberculosis	P63454	isozyme Kas B	486942	2D-image
0.0025	-	eicosanoyl-[acyl-carrier protein]	Mycobacterium tuberculosis	P63454	isozyme Kas A	486942	2D-image
0.0019	-	hexanoyl-[acyl-carrier protein]	Homo sapiens	-	pH 6.8, 37�C	659451	2D-image
0.0075	-	hexanoyl-[acyl-carrier protein]	Spinacia oleracea	-	-	486920	2D-image
0.00032	-	isobutyryl-CoA	Staphylococcus aureus	P99159	synthase III	486946	2D-image
0.00041	-	isobutyryl-CoA	Streptomyces glaucescens	-	synthase III	486933	2D-image
0.004	-	malonyl-CoA	Spinacia oleracea	-	in presence of [acyl-carrier-protein]	486920	2D-image
0.0055	-	malonyl-CoA	Spinacia oleracea	-	-	486924	2D-image
0.016	-	malonyl-CoA	Spinacia oleracea	-	without [acyl-carrier-protein]	486920	2D-image
0.0037	-	malonyl-[acyl-carrier protein]	Streptomyces glaucescens	-	synthase III	486933	2D-image
0.0135	-	malonyl-[acyl-carrier protein]	Mycobacterium tuberculosis	P63454	isozymes Kas A and KasB; isozymes Kas A and KasB	486942	2D-image
0.0186	-	malonyl-[acyl-carrier protein]	Streptococcus pneumoniae	P0A3C5	synthase III	486939	2D-image
0.0508	-	myristoyl-[acyl-carrier protein]	Homo sapiens	-	pH 6.8, 37�C	659451	2D-image
0.0109	-	octanoyl-[acyl-carrier protein]	Homo sapiens	-	pH 6.8, 37�C	659451	2D-image
0.0014	-	Palmitoyl-[acyl-carrier protein]	Mycobacterium tuberculosis	P63454	isozyme Kas B	486942	2D-image
0.0032	-	Palmitoyl-[acyl-carrier protein]	Mycobacterium tuberculosis	P63454	isozyme Kas A	486942	2D-image
0.0036	-	Palmitoyl-[acyl-carrier protein]	Spinacia oleracea	-	synthase II	486924	2D-image
0.0091	-	tetradecanoyl-[acyl-carrier protein]	Spinacia oleracea	-	-	486920	2D-image
0.0139	-	tetradecanoyl-[acyl-carrier protein]	Spinacia oleracea	-	synthase II	486924	2D-image
0.025	-	malonyl-[acyl-carrier protein]	Escherichia coli	-	pH 7.0	486915	2D-image
additional information	-	additional information	Escherichia coli	-	-	486863, 486864	-
additional information	-	additional information	Escherichia coli	-	kinetic data of synthase I and II at different temperatures with various substrates	486916	-
additional information	-	additional information	Spinacia oleracea	-	-	486920	-
additional information	-	additional information	Rattus norvegicus	-	mutants	486944	-
additional information	-	additional information	Plasmodium falciparum	-	kinetics	674777	-

TURNOVER NUMBER [1/s]	TURNOVER NUMBER MAXIMUM[1/s]	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
91.2	-	butanoyl-[acyl-carrier protein]	Homo sapiens	-	pH 6.8, 37�C	659451	2D-image
1.32	-	butyryl-[acyl-carrier protein]	Plasmodium falciparum	-	pH 7.5, 37�C, recombinant enzyme	674777	2D-image
3.07	-	C10-[acyl-carrier protein]	Plasmodium falciparum	-	pH 7.5, 37�C, recombinant enzyme	674777	2D-image
0.69	-	C12-[acyl-carrier protein]	Plasmodium falciparum	-	pH 7.5, 37�C, recombinant enzyme	674777	2D-image
1.28	-	C14-[acyl-carrier protein]	Plasmodium falciparum	-	pH 7.5, 37�C, recombinant enzyme	674777	2D-image
1.32	-	C4-[acyl-carrier protein]	Plasmodium falciparum	-	pH 7.5, 37�C, recombinant enzyme	674777	2D-image
0.96	-	C6-[acyl-carrier protein]	Plasmodium falciparum	-	pH 7.5, 37�C, recombinant enzyme	674777	2D-image
1.63	-	C8-[acyl-carrier protein]	Plasmodium falciparum	-	pH 7.5, 37�C, recombinant enzyme	674777	2D-image
3.07	-	decanoyl-[acyl-carrier protein]	Plasmodium falciparum	-	pH 7.5, 37�C, recombinant enzyme	674777	2D-image
551	-	decanoyl-[acyl-carrier protein]	Homo sapiens	-	pH 6.8, 37�C	659451	2D-image
0.69	-	dodecanoyl-[acyl-carrier protein]	Plasmodium falciparum	-	pH 7.5, 37�C, recombinant enzyme	674777	2D-image
303	-	dodecanoyl-[acyl-carrier protein]	Homo sapiens	-	pH 6.8, 37�C	659451	2D-image
0.01	-	eicosanoyl-[acyl-carrier protein]	Mycobacterium tuberculosis	P63454	isozyme KasB	486942	2D-image
0.075	-	eicosanoyl-[acyl-carrier protein]	Mycobacterium tuberculosis	P63454	isozyme KasA	486942	2D-image
0.96	-	hexanoyl-[acyl-carrier protein]	Plasmodium falciparum	-	pH 7.5, 37�C, recombinant enzyme	674777	2D-image
353	-	hexanoyl-[acyl-carrier protein]	Homo sapiens	-	pH 6.8, 37�C	659451	2D-image
0.0233	-	malonyl-[acyl-carrier protein]	Mycobacterium tuberculosis	P63454	isozyme KasB	486942	2D-image
0.08	-	malonyl-[acyl-carrier protein]	Mycobacterium tuberculosis	P63454	isozyme KasA	486942	2D-image
6	-	myristoyl-[acyl-carrier protein]	Homo sapiens	-	pH 6.8, 37�C	659451	2D-image
1.63	-	octanoyl-[acyl-carrier protein]	Plasmodium falciparum	-	pH 7.5, 37�C, recombinant enzyme	674777	2D-image
66	-	octanoyl-[acyl-carrier protein]	Homo sapiens	-	pH 6.8, 37�C	659451	2D-image
0.0267	-	Palmitoyl-[acyl-carrier protein]	Mycobacterium tuberculosis	P63454	isozyme KasB	486942	2D-image
0.0883	-	Palmitoyl-[acyl-carrier protein]	Mycobacterium tuberculosis	P63454	isozyme KasA	486942	2D-image
1.28	-	tetradecanoyl-[acyl-carrier protein]	Plasmodium falciparum	-	pH 7.5, 37�C, recombinant enzyme	674777	2D-image

kcat/KM VALUE [1/mMs^-1]	kcat/KM VALUE [1/mMs^-1] Maximum	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

Ki VALUE [mM]	Ki VALUE [mM] Maximum	INHIBITOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
0.01	-	thiolactomycin	Plasmodium falciparum	-	pH 7.5, 37�C, recombinant enzyme	674777	2D-image
16	-	C75	Rattus norvegicus	-	pH 7.0, 22�C, inactivation of the whole reaction of FAS	661000	2D-image
additional information	-	additional information	Rattus norvegicus	-	inhibition kinetics	661000	-

IC50 VALUE [mM]	IC50 VALUE [mM] Maximum	INHIBITOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
0.0158	-	cerulenin	Plasmodium falciparum	-	irreversible inhibition, IC50: 0.0158 mM	674777	2D-image
0.016	-	platencin	Staphylococcus aureus	-	exhibits a broad-spectrum Gram-positive antibacterial activity through inhibition of fatty acid biosynthesis, targets the two essential proteins, beta-ketoacyl-[acyl carrier protein] synthase II and III, i.e. FabF and FabH, purified FabH IC50: 0.016 mM, o	676903	2D-image
0.0236	-	thiolactomycin	Plasmodium falciparum	-	competitive inhibition, IC50: 0.0236 mM	674777	2D-image

SPECIFIC ACTIVITY [µmol/min/mg]	SPECIFIC ACTIVITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
3.4e-06	-	Mycobacterium tuberculosis	P63454	substrate palmitoyl-CoA	486942
6e-06	-	Mycobacterium tuberculosis	P63454	substrate palmitoyl-CoA	486942
3.2e-05	-	Mycobacterium tuberculosis	P63454	substrate palmitoyl-[acyl-carrier-protein]	486942
8.8e-05	-	Mycobacterium tuberculosis	P63454	substrate palmitoyl-[acyl-carrier-protein]	486942
0.0017	-	Mycobacterium tuberculosis	-	substrate hexanoyl-[acyl-carrier protein], pH 7.0, 37�C	659493
0.005	-	Streptococcus pneumoniae	P0A3C5	synthase III	486939
0.0055	-	Mycobacterium tuberculosis	-	substrate octanoyl-[acyl-carrier protein], pH 7.0, 37�C	659493
0.0062	-	Mycobacterium tuberculosis	-	substrate decanoyl-[acyl-carrier protein], pH 7.0, 37�C	659493
0.0072	-	Mycobacterium tuberculosis	-	substrate dodecanoyl-[acyl-carrier protein], pH 7.0, 37�C	659493
0.0083	-	Spinacia oleracea	-	purified enzyme	486920
0.009	-	Mycobacterium tuberculosis	-	substrate eicosanoyl-[acyl-carrier-protein], pH 7.0, 37�C	659493
0.0093	-	Mycobacterium tuberculosis	-	substrate palmitoyl-[acyl-carrier protein], pH 7.0, 37�C	659493
0.0095	-	Mycobacterium tuberculosis	-	substrate myristoyl-[acyl-carrier protein], pH 7.0, 37�C	659493
0.0104	-	Mycobacterium tuberculosis	-	substrate octadecanoyl-[acyl-carrier-protein], pH 7.0, 37�C	659493
0.105	-	Plasmodium falciparum	-	purified recombinant enzyme with substrate C6-[acyl-carrier-protein]	674777
0.403	-	Escherichia coli	-	purified enzyme	486914
0.8	-	Brassica napus	-	purified synthase I	486922
3.22	-	Euglena gracilis	-	-	285676
3.81	-	Escherichia coli	-	purified enzyme	486915
5.5	-	Escherichia coli	-	synthase I	486916
6.3	-	Escherichia coli	-	synthase II	486916
7	-	Spinacia oleracea	-	purified native enzyme	486931
7.5	-	Escherichia coli	-	purified synthase II	486919
8.04	-	Petroselinum crispum	-	partially purified enzyme	486921
9	-	Escherichia coli	-	purified synthase I	486919
9.7	-	Escherichia coli	-	-	486917
14	-	Escherichia coli	-	-	486863
additional information	-	Carthamus tinctorius, Cuphea lutea, Pisum sativum	-	fatty acid synthase activity, malonyl-CoA incorporation	285675
additional information	-	Spinacia oleracea	-	-	285675
additional information	-	Brassica napus	-	-	486922, 486923
additional information	-	Spinacia oleracea	-	-	486924
additional information	-	Spinacia oleracea	-	comparison of activity, recombinant and native enzyme	486931
additional information	-	Brassica napus, Escherichia coli	-	wild-type and transgenic Brassica napus plants, expressing the E. coli synthase III	486932
additional information	-	Bacillus subtilis, Escherichia coli	-	-	486937
additional information	-	Rattus norvegicus	-	mutants	486944
additional information	-	Arabidopsis thaliana	Q8L3X9	enzyme from fresh extract synthesizes prominent amounts of C14 and C16 acyl chains. After storage at -20�C for one or several weeks, enzyme synthesizes predominantly C8 acyl chains	659442
additional information	-	Escherichia coli	-	transfer of [3H]myristate from ACP to wild-type and Lys328 mutant KAS I	673576
additional information	-	Plasmodium falciparum	-	-	674777
additional information	-	Trypanosoma brucei	Q586W9	-	674882
additional information	-	Staphylococcus aureus	-	development of an elongation assay with FabF, EC 2.3.1.179, and FabH	676903

pH OPTIMUM	pH MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
5.5	6.1	Escherichia coli	-	synthase II	486919
6	7.5	Helianthus annuus	-	-	674095
6.3	-	Brassica napus	-	-	486923
6.6	7.7	Streptomyces glaucescens	-	synthase III	486933
6.8	-	Mycobacterium tuberculosis	P63454	isozyme KasA, HEPES buffer	486942
6.8	-	Escherichia coli	-	assay at	486943
6.8	-	Escherichia coli	-	decarboxylation assay at, inactive at pH 4.0-5.0	673576
7	7.8	Escherichia coli	-	-	486863, 486915
7	-	Escherichia coli	-	assay at	486914
7	-	Streptomyces glaucescens	-	assay at	486933
7	-	Bacillus subtilis, Escherichia coli	-	assay at	486937
7	-	Mycobacterium tuberculosis	P63454	isozyme KasB, phosphate buffer	486942
7	-	Rattus norvegicus	-	assay at	661000
7	-	Staphylococcus aureus	-	assay at	676903
7.2	-	Escherichia coli	-	synthase I	486919
7.2	-	Staphylococcus aureus	P99159	synthase III	486946
7.5	-	Coriandrum sativum	-	assay at	486948
7.5	-	Plasmodium falciparum	-	assay at	674777
7.5	-	Trypanosoma brucei	Q586W9	assay at	674882
8	-	Allium ampeloprasum	-	assay at	486935
8.1	8.5	Spinacia oleracea	-	-	486924

pH RANGE	pH RANGE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
5.2	7.6	Escherichia coli	-	synthase II, about half-maximal activity at pH 5.2 and pH 7.6	486919
5.6	7.2	Brassica napus	-	synthase II, about half-maximal activity at pH 5.6 and pH 7.2	486923
5.8	8	Staphylococcus aureus	P99159	synthase III	486946
5.9	6.8	Brassica napus	-	synthase I, about half-maximal activity at pH 5.9 and pH 6.8	486923
6	8.2	Spinacia oleracea	-	-	486920
6.3	8	Escherichia coli	-	synthase I, about half-maximal activity at pH 6.3 and pH 8.0	486919
6.5	8	Escherichia coli	-	-	486863
6.5	8	Escherichia coli	-	sharp drop in activity below pH 6.5 and above pH 8.0	486915
6.8	9.1	Spinacia oleracea	-	-	486924

TEMPERATURE OPTIMUM	TEMPERATURE OPTIMUM MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
20	-	Escherichia coli	-	assay at	486943
22	-	Rattus norvegicus	-	assay at room temperature	661000
22	-	Escherichia coli	-	assay at room temperature	673576
25	-	Escherichia coli	-	assay at	486914, 486915
25	-	Spinacia oleracea	-	assay at	486920
27	-	Escherichia coli	-	assay at	486916
30	-	Escherichia coli	-	assay at	486864
30	-	Petroselinum crispum	-	assay at	486921
30	-	Streptomyces glaucescens	-	assay at	486933
30	-	Staphylococcus aureus	-	assay at	676903
35	-	Euglena gracilis	-	assay at	285676
37	-	Escherichia coli	-	assay at	486916, 486917
37	-	Spinacia oleracea	-	assay at	486920
37	-	Brassica napus	-	assay at	486923
37	-	Bacillus subtilis, Escherichia coli	-	assay at	486937
37	-	Mycobacterium tuberculosis	P63454	;	486942
37	-	Plasmodium falciparum	-	assay at	674777
37	-	Trypanosoma brucei	Q586W9	assay at	674882

TEMPERATURE RANGE	TEMPERATURE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
30	42	Mycobacterium tuberculosis	P63454	30-50% activity at 30 and 42�C; 30-50% activity at 30 and 42�C	486942

pI VALUE	pI VALUE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

SOURCE TISSUE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	SOURCE
brain	Homo sapiens	-	low level	659451
cell suspension culture	Petroselinum crispum	-	-	486921
epidermis	Allium ampeloprasum	-	sheath and lamina, and underlying parenchyma	486935
heart	Homo sapiens	-	most abundant	659451
kernel	Helianthus annuus	-	-	674095
kidney	Homo sapiens	-	-	659451
leaf	Spinacia oleracea	-	-	285675, 486920, 486924, 486925, 486931
leaf	Brassica juncea, Carthamus tinctorius, Cuphea lutea, Pisum sativum	-	-	285675
leaf	Hordeum vulgare	-	-	486925
leaf	Allium ampeloprasum	-	-	486935
liver	Homo sapiens	-	-	659451
lung	Homo sapiens	-	low level	659451
placenta	Homo sapiens	-	low level	659451
seed	Carthamus tinctorius	-	germinating	285669, 285675
seed	Brassica juncea, Spinacia oleracea	-	germinating	285675
seed	Brassica napus	-	germinating	486922, 486923
seed	Cuphea lanceolata	-	embryo	486947
seed	Coriandrum sativum	-	germinating; specific isoform	486948
skeletal muscle	Homo sapiens	-	most abundant	659451
spleen	Homo sapiens	-	low level	659451

LOCALIZATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	GeneOntology No.	LITERATURE	SOURCE
chloroplast	Euglena gracilis	-	-	9507	285676
chloroplast	Hordeum vulgare, Spinacia oleracea	-	stroma	9507	486925
chloroplast	Coriandrum sativum	-	-	9507	486948
cytosol	Escherichia coli	-	-	5829	486864
endoplasmic reticulum membrane	Helianthus annuus	-	-	5789	674095
microsome	Helianthus annuus	-	-	-	674095
microsome	Trypanosoma brucei	Q586W9	enzyme in the fatty acid synthase complex	-	674882
mitochondrion	Arabidopsis thaliana	Q8L3X9	-	5739	659442
mitochondrion	Homo sapiens	-	-	5739	659451
mitochondrion	Trypanosoma brucei	Q586W9	enzyme in the elongase complex	5739	674882
mitochondrion	Homo sapiens	Q9NWU1	beta-ketoacyl [ACP] synthase from the mitochondrial type II fatty acid synthase	5739	677030

PDB	SCOP	CATH	ORGANISM
2ebd, download	SCOP (2ebd)	CATH (2ebd)	Aquifex aeolicus (strain VF5)
1w0i, download	SCOP (1w0i)	CATH (1w0i)	Arabidopsis thaliana
2ix4, download	SCOP (2ix4)	CATH (2ix4)	Arabidopsis thaliana
3lrf, download	SCOP (3lrf)	CATH (3lrf)	Brucella abortus (strain 2308)
3u0e, download	SCOP (3u0e)	CATH (3u0e)	Brucella abortus (strain 2308)
3u0f, download	SCOP (3u0f)	CATH (3u0f)	Brucella abortus (strain 2308)
3kzu, download	SCOP (3kzu)	CATH (3kzu)	Brucella melitensis biotype 1 (strain 16M / ATCC 23456 / NCTC 10094)
4efi, download	SCOP (4efi)	CATH (4efi)	Burkholderia xenovorans (strain LB400)
1b3n, download	SCOP (1b3n)	CATH (1b3n)	Escherichia coli (strain K12)
1dd8, download	SCOP (1dd8)	CATH (1dd8)	Escherichia coli (strain K12)
1ebl, download	SCOP (1ebl)	CATH (1ebl)	Escherichia coli (strain K12)
1ek4, download	SCOP (1ek4)	CATH (1ek4)	Escherichia coli (strain K12)
1f91, download	SCOP (1f91)	CATH (1f91)	Escherichia coli (strain K12)
1fj4, download	SCOP (1fj4)	CATH (1fj4)	Escherichia coli (strain K12)
1fj8, download	SCOP (1fj8)	CATH (1fj8)	Escherichia coli (strain K12)
1g5x, download	SCOP (1g5x)	CATH (1g5x)	Escherichia coli (strain K12)
1h4f, download	SCOP (1h4f)	CATH (1h4f)	Escherichia coli (strain K12)
1hn9, download	SCOP (1hn9)	CATH (1hn9)	Escherichia coli (strain K12)
1hnd, download	SCOP (1hnd)	CATH (1hnd)	Escherichia coli (strain K12)
1hnh, download	SCOP (1hnh)	CATH (1hnh)	Escherichia coli (strain K12)
1hnj, download	SCOP (1hnj)	CATH (1hnj)	Escherichia coli (strain K12)
1hnk, download	SCOP (1hnk)	CATH (1hnk)	Escherichia coli (strain K12)
1kas, download	SCOP (1kas)	CATH (1kas)	Escherichia coli (strain K12)
1mzs, download	SCOP (1mzs)	CATH (1mzs)	Escherichia coli (strain K12)
2aq7, download	SCOP (2aq7)	CATH (2aq7)	Escherichia coli (strain K12)
2aqb, download	SCOP (2aqb)	CATH (2aqb)	Escherichia coli (strain K12)
2buh, download	SCOP (2buh)	CATH (2buh)	Escherichia coli (strain K12)
2bui, download	SCOP (2bui)	CATH (2bui)	Escherichia coli (strain K12)
2byw, download	SCOP (2byw)	CATH (2byw)	Escherichia coli (strain K12)
2byx, download	SCOP (2byx)	CATH (2byx)	Escherichia coli (strain K12)
2byy, download	SCOP (2byy)	CATH (2byy)	Escherichia coli (strain K12)
2byz, download	SCOP (2byz)	CATH (2byz)	Escherichia coli (strain K12)
2bz3, download	SCOP (2bz3)	CATH (2bz3)	Escherichia coli (strain K12)
2bz4, download	SCOP (2bz4)	CATH (2bz4)	Escherichia coli (strain K12)
2eft, download	SCOP (2eft)	CATH (2eft)	Escherichia coli (strain K12)
2gfv, download	SCOP (2gfv)	CATH (2gfv)	Escherichia coli (strain K12)
2gfw, download	SCOP (2gfw)	CATH (2gfw)	Escherichia coli (strain K12)
2gfx, download	SCOP (2gfx)	CATH (2gfx)	Escherichia coli (strain K12)
2gfy, download	SCOP (2gfy)	CATH (2gfy)	Escherichia coli (strain K12)
2gyo, download	SCOP (2gyo)	CATH (2gyo)	Escherichia coli (strain K12)
2vb7, download	SCOP (2vb7)	CATH (2vb7)	Escherichia coli (strain K12)
2vb8, download	SCOP (2vb8)	CATH (2vb8)	Escherichia coli (strain K12)
2vb9, download	SCOP (2vb9)	CATH (2vb9)	Escherichia coli (strain K12)
2vba, download	SCOP (2vba)	CATH (2vba)	Escherichia coli (strain K12)
1h4f, download	SCOP (1h4f)	CATH (1h4f)	Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
2buh, download	SCOP (2buh)	CATH (2buh)	Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
2bui, download	SCOP (2bui)	CATH (2bui)	Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
2byw, download	SCOP (2byw)	CATH (2byw)	Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
2byx, download	SCOP (2byx)	CATH (2byx)	Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
2byy, download	SCOP (2byy)	CATH (2byy)	Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
2byz, download	SCOP (2byz)	CATH (2byz)	Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
2bz3, download	SCOP (2bz3)	CATH (2bz3)	Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
2bz4, download	SCOP (2bz4)	CATH (2bz4)	Escherichia coli O6:H1 (strain CFT073 / ATCC 700928 / UPEC)
2c9h, download	SCOP (2c9h)	CATH (2c9h)	Homo sapiens
2iwy, download	SCOP (2iwy)	CATH (2iwy)	Homo sapiens
2iwz, download	SCOP (2iwz)	CATH (2iwz)	Homo sapiens
3tjm, download	SCOP (3tjm)	CATH (3tjm)	Homo sapiens
1hzp, download	SCOP (1hzp)	CATH (1hzp)	Mycobacterium tuberculosis
1m1m, download	SCOP (1m1m)	CATH (1m1m)	Mycobacterium tuberculosis
1u6e, download	SCOP (1u6e)	CATH (1u6e)	Mycobacterium tuberculosis
1u6s, download	SCOP (1u6s)	CATH (1u6s)	Mycobacterium tuberculosis
2ahb, download	SCOP (2ahb)	CATH (2ahb)	Mycobacterium tuberculosis
2aj9, download	SCOP (2aj9)	CATH (2aj9)	Mycobacterium tuberculosis
2gp6, download	SCOP (2gp6)	CATH (2gp6)	Mycobacterium tuberculosis
2qnx, download	SCOP (2qnx)	CATH (2qnx)	Mycobacterium tuberculosis
2qny, download	SCOP (2qny)	CATH (2qny)	Mycobacterium tuberculosis
2qnz, download	SCOP (2qnz)	CATH (2qnz)	Mycobacterium tuberculosis
2qo0, download	SCOP (2qo0)	CATH (2qo0)	Mycobacterium tuberculosis
2qo1, download	SCOP (2qo1)	CATH (2qo1)	Mycobacterium tuberculosis
2qx1, download	SCOP (2qx1)	CATH (2qx1)	Mycobacterium tuberculosis
2wgd, download	SCOP (2wgd)	CATH (2wgd)	Mycobacterium tuberculosis
2wge, download	SCOP (2wge)	CATH (2wge)	Mycobacterium tuberculosis
2wgf, download	SCOP (2wgf)	CATH (2wgf)	Mycobacterium tuberculosis
2wgg, download	SCOP (2wgg)	CATH (2wgg)	Mycobacterium tuberculosis
3led, download	SCOP (3led)	CATH (3led)	Rhodopseudomonas palustris (strain ATCC BAA-98 / CGA009)
2vkz, download	SCOP (2vkz)	CATH (2vkz)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
2was, download	SCOP (2was)	CATH (2was)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
2wat, download	SCOP (2wat)	CATH (2wat)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
1zow, download	SCOP (1zow)	CATH (1zow)	Staphylococcus aureus (strain MW2)
2gqd, download	SCOP (2gqd)	CATH (2gqd)	Staphylococcus aureus (strain MW2)
1ox0, download	SCOP (1ox0)	CATH (1ox0)	Streptococcus pneumoniae
1oxh, download	SCOP (1oxh)	CATH (1oxh)	Streptococcus pneumoniae
2alm, download	SCOP (2alm)	CATH (2alm)	Streptococcus pneumoniae
2rjt, download	SCOP (2rjt)	CATH (2rjt)	Streptococcus pneumoniae
1tqy, download	SCOP (1tqy)	CATH (1tqy)	Streptomyces coelicolor (strain ATCC BAA-471 / A3(2) / M145)
1mzj, download	SCOP (1mzj)	CATH (1mzj)	Streptomyces lividans
1e5m, download	SCOP (1e5m)	CATH (1e5m)	Synechocystis sp. (strain PCC 6803 / Kazusa)
1ub7, download	SCOP (1ub7)	CATH (1ub7)	Thermus thermophilus
3row, download	SCOP (3row)	CATH (3row)	Xanthomonas campestris pv. campestris (strain ATCC 33913 / NCPPB 528 / LMG 568)
3fk5, download	SCOP (3fk5)	CATH (3fk5)	Xanthomonas oryzae pv. oryzae (strain KACC10331 / KXO85)

MOLECULAR WEIGHT	MOLECULAR WEIGHT MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
38000	-	Streptomyces peucetius	-	synthase III-like polyketid synthase, gel filtration, amino acid sequence determination	486934
56000	-	Spinacia oleracea	-	synthase I, gel filtration	285675, 486920, 486924
57500	-	Spinacia oleracea	-	synthase II, gel filtration	285675, 486924
60000	-	Streptococcus pneumoniae	P0A3C5	synthase III, gel filtration	486939
63000	-	Spinacia oleracea	-	synthase III, gel filtration	486931
66000	-	Escherichia coli	-	equilibrium centrifugation	486863
66000	-	Escherichia coli	-	synthase I, gel filtration	486919
67600	-	Escherichia coli	-	gel filtration	486914
67600	-	Staphylococcus aureus	P99159	gel filtration	486946
70000	-	Petroselinum crispum	-	about, gel filtration	486921
72000	-	Streptomyces glaucescens	-	recombinant enzyme, gel filtration	486933
76000	-	Escherichia coli	-	synthase II, gel filtration	486919
80000	-	Escherichia coli	-	synthase I, sedimentation equilibrium method	486916
85000	-	Escherichia coli	-	synthase II, sedimentation equilibrium method	486916
86700	-	Brassica napus	-	synthase I, gel filtration	486922, 486923
87400	-	Brassica napus	-	synthase II, gel filtration	486923
87800	-	Brassica napus	-	synthase II, gel filtration	486922
90000	-	Hordeum vulgare, Spinacia oleracea	-	gel filtration	486925
118000	-	Euglena gracilis	-	gel filtration	285676
additional information	-	Escherichia coli	-	amino acid composition, synthase I and II	486916
additional information	-	Bacillus subtilis, Escherichia coli	-	amino acid sequence alignment	486937
additional information	-	Escherichia coli, Haemophilus influenzae	-	amino acid sequence alignment	486939
additional information	-	Streptococcus pneumoniae	P0A3C5	amino acid sequence alignment	486939
additional information	-	Escherichia coli	-	synthase I mutants	486943
additional information	-	Staphylococcus aureus	P99159	amino acid sequence alignment	486946
additional information	-	Coriandrum sativum	-	amino acid sequence alignment	486948

SUBUNITS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
?	Bacillus subtilis	-	x * 39800, FabH2, SDS-PAGE; x * 42700, FabH1, SDS-PAGE	486937
?	Arabidopsis thaliana	Q8L3X9	x * 47000, SDS-PAGE	659442
?	Homo sapiens	-	x * 45800, SDS-PAGE, recombinant protein without N-terminal targeting sequence, with His-tag	659451
dimer	Escherichia coli	-	2 * 35000, SDS-PAGE; 2 * 37000, gel filtration after 6 M guanidinium chloride treatment	486863
dimer	Escherichia coli	-	2 * 34000, SDS-PAGE	486864
dimer	Escherichia coli	-	2 * 43000-44000, synthase I, SDS-PAGE; 2 * 44000-45000, synthase II, SDS-PAGE	486916
dimer	Brassica napus	-	2 * 43000, SDS-PAGE	486922, 486923
dimer	Hordeum vulgare	-	2 * 45000, SDS-PAGE; 2 * 46000, alpha2, synthase I, SDS-PAGE	486925
dimer	Spinacia oleracea	-	2 * 46000, SDS-PAGE	486925
dimer	Spinacia oleracea	-	2 * 40500, synthase III, SDS-PAGE	486931
dimer	Streptomyces glaucescens	-	2 * 37000, recombinant synthase III, SDS-PAGE	486933
dimer	Synechocystis sp.	-	crystal structure analysis of subunits	486940
dimer	Staphylococcus aureus	P99159	2 * 37000, recombinant synthase III, SDS-PAGE	486946
dimer	Arabidopsis thaliana	Q8L3X9	crystallization data	658746
dimer	Homo sapiens	Q9NWU1	secondary structure, a dimer with an alpha-beta-alpha-beta-alpha-thiolase fold capped by an alpha-helical region connecting the strands of the N-terminal beta-sheet, crystal structure analysis	677030
monomer	Streptomyces peucetius	-	1 * 38000, synthase III-like polyketid synthase, SDS-PAGE	486934
additional information	Escherichia coli	-	vertebrates, yeast and Mycobacteria fatty acid synthetases contain all individual activities on one or two multifunctional polypeptide chains, plant, Escherichia coli and other prokaryotic fatty acid synthases are non-associated systems of individual enzymes	486863, 486864
additional information	Clostridium kluyveri	-	vertebrates, yeast and Mycobacteria fatty acid synthetases contain all individual activities on one or two multifunctional polypeptide chains, plant, Escherichia coli and other prokaryotic fatty acid synthases are non-associated systems of individual enzymes	486863
additional information	Escherichia coli	-	synthase I mutants	486943
additional information	Escherichia coli	P0A953	comparison of crystal structures of the active sites of Escherichia coli KAS I with human cytosolic acetoacetyl-CoA thiolase and bacterial thiolase, active sites with four highly conserved loop structures, overview	659726
additional information	Escherichia coli	-	comparison of active sites in isoforms KAS I, KAS II and related enzymes	660470
additional information	Escherichia coli	-	analysis of wild-type and mutant active sites with bound substrates at different pH, overview	673576

POSTTRANSLATIONAL MODIFICATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
proteolytic modification	Arabidopsis thaliana	Q8L3X9	20 amino acid mitochondrial targeting sequence	659442
proteolytic modification	Homo sapiens	-	mitochondrial targeting sequence of 38 residues	659451

Crystallization/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
-	Arabidopsis thaliana	Q8L3X9	658746
-	Escherichia coli	-	486863, 486864
comparison of crystal structures of the active sites of Escherichia coli KAS I with human cytosolic acetoacetyl-CoA thiolase and bacterial thiolase, overview	Escherichia coli	P0A953	659726
purified recombinant wild-type and mutant enzymes, free or bound to C8 and C12, respectively, X-ray diffraction structure determination and analysis at 1.8-2.4 A resolution	Escherichia coli	-	673576
structure analysis, three-dimensional model of enzyme complexed with inhibitors thiolactomycin and cerulenin	Escherichia coli	-	486941
purified recombinant enzyme free or complexed with acyl-CoA, hanging drop vapour diffusion method, 0.002 ml of protein solution, containing 0.087 mg of protein with or without 4 mM hexanoyl-CoA, mixed with 0.002 ml of reservoir solution containing 24% w/v PEG 3350 and 0.2 M NH4Cl, 5-8 days at room temperature, rod-shaped single crystals, X-ray diffraction structure determination and analysis at 1.6 A resolution, structure modeling	Homo sapiens	Q9NWU1	677030
in complex with lauroyl-CoA	Mycobacterium tuberculosis	-	659724
-	Staphylococcus aureus	-	660466
-	Streptococcus pneumoniae	-	659029
hanging drop vapour diffusion method, protein solution: 25 mM Tris-HCl, pH 8.0, 300 mM NaCl, 75 mM imidazole, reservoir solution: 0.085 M tri-sodium citrate, 0.17 M ammonium acetate, 22% w/v polyethylene glycol 4000, 15% v/v glycerol as cryo-protectant, 15% w/v 1,2,3-heptanetriol, x-ray structure analysis, three-dimensional model	Synechocystis sp.	-	486940

pH STABILITY	pH STABILITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

TEMPERATURE STABILITY	TEMPERATURE STABILITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
43	-	Escherichia coli	-	t1/2 synthase I: 18 min, t1/2 synthase II: 81 min	486919
100	-	Escherichia coli	-	inactivation after 1 min	486864

GENERAL STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
dithiothreitol stabilizes	Brassica napus	-	486923
proteinase inhibitors stabilize during purification	Brassica napus	-	486923
Triton X-100 facilitates extraction from oil seeds	Brassica napus	-	486923
unstable during purification	Brassica napus	-	486922
2-mercaptoethanol stabilizes	Escherichia coli	-	486914
EDTA stabilizes	Escherichia coli	-	486914
unstable during purification	Escherichia coli	-	486914
freezing and thawing inactivates	Euglena gracilis	-	285676
dithiothreitol stabilizes	Spinacia oleracea	-	486931
glycerol stabilizes	Spinacia oleracea	-	486931
Triton X-100 stabilizes	Spinacia oleracea	-	486931

ORGANIC SOLVENT	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

OXIDATION STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
oxidation by formic acid	Escherichia coli	-	486918

STORAGE STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
0�C, unstable in crude extracts lacking glycerol. Enzyme from fresh extract synthesizes prominent amounts of C14 and C16 acyl chains. After storage at -20�C for one or several weeks, enzyme synthesizes predominantly C8 acyl chains	Arabidopsis thaliana	Q8L3X9	659442
-70� to -20�C, 90% loss of activity within 7 days	Brassica napus	-	486923
-20�C, 90% loss of activity within 1 year	Escherichia coli	-	486864
-20�C, at least 1 month	Escherichia coli	-	486864
-20�C, complete loss of activity in the presence of EDTA and 2-mercaptoethanol	Escherichia coli	-	486914
-20�C, several months	Escherichia coli	-	486915
-20�C, t1/2: 1 month	Spinacia oleracea	-	486920
-70�C, at least 1 month	Spinacia oleracea	-	486924
-80�C, several weeks in the presence of 20% v/v glycerol, 0.1% Triton X-100 and dithiothreitol	Spinacia oleracea	-	486931

Purification/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
recombinant enzyme	Arabidopsis thaliana	Q8L3X9	659442
recombinant His-tagged proteins FabH1 and FabH2 from E. coli	Bacillus subtilis	-	486937
-	Brassica napus	-	486923
synthases I and II	Brassica napus	-	486922
recombinant His-tagged seed-specific isoform from E. coli	Coriandrum sativum	-	486948
recombinant His-tagged synthase IV from E. coli	Cuphea lanceolata	-	486947
-	Escherichia coli	-	486863, 486864, 486914
isolation of hexanoyl-enzyme intermediate complex	Escherichia coli	-	486918
partial	Escherichia coli	-	486915
recombinant synthase I and mutants as His-tagged protein from E. coli	Escherichia coli	-	486943
recombinant synthases I, i.e. FabA, II, i.e. FabB, and III, i.e.FabH	Escherichia coli	-	486941
synthases I and II	Escherichia coli	-	486916, 486919
-	Euglena gracilis	-	285676
recombinant His-tagged protein from E. coli	Haemophilus influenzae	-	486939
native enzyme from kernel microsomes, solubilization and separation from KAS II by treatment with Triton X-100 and anion exchange chromatography	Helianthus annuus	-	674095
recombinant His-tagged enzyme from Escherichia coli strains XL-1 blue and M15	Homo sapiens	Q9NWU1	677030
3 proteins alpha2, alpha,beta and beta2	Hordeum vulgare	-	486925
recombinant His-tagged protein from E. coli; recombinant His-tagged protein from E. coli	Mycobacterium tuberculosis	P63454	486942
partial	Petroselinum crispum	-	486921
recombinant soluble NusA-fusion protein FabBF from Escherichia coli strain BL21(DE3) by and gel filtration	Plasmodium falciparum	-	674777
native fatty acid synthase from liver in several steps including gel filtration as the last step	Rattus norvegicus	-	661000
synthase I	Spinacia oleracea	-	486920
synthase I; synthases II	Spinacia oleracea	-	285675
synthase III, native wild-type and recombinant from E. coli E103S	Spinacia oleracea	-	486931
synthases II	Spinacia oleracea	-	486924
recombinant His-tagged synthase III from E. coli	Staphylococcus aureus	P99159	486946
recombinant His-tagged synthase III from E. coli	Streptococcus pneumoniae	P0A3C5	486939
recombinant from E. coli as His-tagged protein	Streptomyces glaucescens	-	486933
recombinant from E. coli as His-tagged protein; wild-type and mutants	Streptomyces glaucescens	-	486938
synthase III-like polyketid synthase recombinant from Streptomyces lividans	Streptomyces peucetius	-	486934
synthase II, recombinant from E. coli	Synechocystis sp.	-	486936

Cloned/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
complements KAS II defect in Escherichia coli	Arabidopsis thaliana	Q8L3X9	659442
expression of His-tagged FabH1 and FabH2 in Escherichia coli INValphaF'	Bacillus subtilis	-	486937
construction of a cDNA library from developing seeds, DNA sequence analysis, expression as His-tagged enzyme in Escherichia coli	Coriandrum sativum	-	486948
expression of His-tagged synthase IV in Escherichia coli BL21 (DE3)	Cuphea lanceolata	-	486947
expression of synthase I and mutants as His-tagged proteins in Escherichia coli	Escherichia coli	-	486943
synthase III, overexpression in Escherichia coli strain JM101, construction of binary expression vectors for transformation of Brassica napus	Escherichia coli	-	486932
synthases I, II, and III, recombinant expression	Escherichia coli	-	486941
expression as His-tagged protein in Escherichia coli	Haemophilus influenzae	-	486939
expression of the His-tagged enzyme in Escherichia coli strains XL-1 blue and M15	Homo sapiens	Q9NWU1	677030
cDNA cloned into lambda2AB-II	Hordeum vulgare	-	486925
expression of KasA and KasB as His-tagged protein in Escherichia coli; expression of KasA and KasB as His-tagged protein in Escherichia coli	Mycobacterium tuberculosis	P63454	486942
gene fabBF, DNA and amino acid sequence determination and analysis, sequence comparisons, expression of the soluble NusA-fusion protein FabBF in Escherichia coli strain BL21(DE3)	Plasmodium falciparum	-	674777
synthase III, overexpression of His-tagged FabH in Escherichia coli BL21 (DE3)	Staphylococcus aureus	P99159	486946
overexpression of His-tagged FabH in Escherichia coli BL21 (DE3)	Streptococcus pneumoniae	P0A3C5	486939
overexpression of fabH in Escherichia coli as His-tagged protein, synthase III	Streptomyces glaucescens	-	486933
plasmid-based expression of synthase III wild-type and mutants in Streptomyces glaucescens and His-tagged in Escherichia coli	Streptomyces glaucescens	-	486938
expression of synthase III-like polyketid synthase in Streptomyces lividans	Streptomyces peucetius	-	486934
expression in Escherichia coli	Synechocystis sp.	-	486940
expression of wild-type and mutant synthase II in Escherichia coli	Synechocystis sp.	-	486936

EXPRESSION	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

ENGINEERING	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
C163A	Escherichia coli	-	synthase I, site-directed mutagenesis, active site mutant, no activity, but decarboxylates malonyl-[acyl-carrier-protein]	486943
C163S	Escherichia coli	-	synthase I, site-directed mutagenesis, active site mutant, increased activity, decarboxylates malonyl-[acyl-carrier-protein]	486943
D306A	Escherichia coli	-	synthase I, site-directed mutagenesis, active site mutant, increased activity	486943
E309A	Escherichia coli	-	synthase I, site-directed mutagenesis, active site mutant, reduced activity	486943
H298A	Escherichia coli	-	synthase I, site-directed mutagenesis, active site mutant, reduced activity	486943
H298E	Escherichia coli	-	site-directed mutagenesis, the mutant is decarboxylation deficient, residual decarboxylase activity in the range of pH 6.0�8.0, crystal structure determination with the mutant enzyme free or bound to C12, comparison to the wild-type enzyme structure	673576
H298Q	Escherichia coli	-	site-directed mutagenesis, the mutant is completely decarboxylation deficient, crystal structure determination with the mutant enzyme free or bound to C12, comparison to the wild-type enzyme structure	673576
H333A	Escherichia coli	-	synthase I, site-directed mutagenesis, active site mutant, increased activity	486943
K328A	Escherichia coli	-	synthase I, site-directed mutagenesis, active site mutant, reduced activity	486943
K328A	Escherichia coli	-	site-directed mutagenesis, the mutant is completely decarboxylation deficient, crystal structure determination with the mutant enzyme free or bound to C12, comparison to the wild-type enzyme structure	673576
K328E	Escherichia coli	-	site-directed mutagenesis, the mutant is almost completely decarboxylation deficient	673576
K328F	Escherichia coli	-	site-directed mutagenesis, the mutant is completely decarboxylation deficient	673576
K328H	Escherichia coli	-	site-directed mutagenesis, the mutant is completely decarboxylation deficient	673576
K328I	Escherichia coli	-	site-directed mutagenesis, the mutant is almost completely decarboxylation deficient	673576
R161A	Mycobacterium tuberculosis	-	69.6% of wild-type activity	659493
R46A	Mycobacterium tuberculosis	-	7.3% of wild-type activity	659493
R46A/R161A	Mycobacterium tuberculosis	-	0.3% of wild-type activity	659493
T97F	Mycobacterium tuberculosis	-	no enzymic activity	659493
W42A	Mycobacterium tuberculosis	-	21.5% of wild-type activity	659493
W42A/R161A	Mycobacterium tuberculosis	-	0.2% of wild-type activity	659493
C122A	Streptomyces glaucescens	-	site-directed mutagenesis, 75% enhanced production of straight-chain fatty acids relative to branched-chain fatty acids compared to wild-type	486938
C122Q	Streptomyces glaucescens	-	site-directed mutagenesis, 500% enhanced production of straight-chain fatty acids relative to branched-chain fatty acids compared to wild-type	486938
C122S	Streptomyces glaucescens	-	site-directed mutagenesis, enhanced production of straight-chain fatty acids relative to branched-chain fatty acids compared to wild-type	486938
A193M	Synechocystis sp.	-	site-directed mutagenesis, hydrophobic acyl-binding pocket is decreased in size by 2.55fold, 12fold reduced catalytic activity	486936
additional information	Brassica napus	-	transformation of Brassica napus via Agrobacterium tumefaciens infection with binary vector overexpressing Escherichia coli fabH gene, targeted expression in cytoplasm and plastids is seed specific, modified fatty acid profile of seed oil	486932
K328R	Escherichia coli	-	site-directed mutagenesis, the mutant is almost completely decarboxylation deficient, crystal structure determination with the free mutant enzyme, comparison to the wild-type enzyme structure	673576
additional information	Escherichia coli	-	transformation of Brassica napus via Agrobacterium tumefaciens infection with binary vector overexpressing Escherichia coli fabH gene, targeted expression in cytoplasm and plastids is seed specific, modified fatty acid profile of seed oil	486932
additional information	Escherichia coli	-	introduction of temperature-sensitive mutation in fabB into strain K27 by phage P1-mediated transduction using closely linked Tn10 insertion as selectable marker. A fabB mutant strain accumulates less cis-5-dodecenoic acid than the parental wild-type strain. The wild-type strain also accumulates 2.7fold more cis-7-tetradecenoate than the fabB(Ts) strain. The combination of null mutations in fadD and fabA or fabB is synthetically lethal even in the presence of oleic acid supplementation. A temperature-sensitive mutation is used rather than null mutations because a strain having fabB null mutation has an obligate requirement for an unsaturated fatty acid and FabD is required for uptake of the supplement	704553
additional information	Lactococcus lactis	-	in-frame deletion of enzyme active site region, mutant strain retains about 10% enzymic activity and fails to grow in the absence of supplementation with exogenous long-chain fatty acids. Mutant requires only long-chain unsaturated fatty acids, no saturated fatty acids	659294
C161Q	Rattus norvegicus	-	site-directed mutagenesis, active site Cys mutant, no condensation activity, but 377fold increased decarboxylation activity	486944
additional information	Rattus norvegicus	-	triple and quadruple mutants of fatty acid synthase complex including mutation sites in the beta-ketoacyl-[acyl-carrier-protein] synthase. e.g. H293A, H331A, K326A,S581A, kinetics, overview	486944
I108F	Synechocystis sp.	-	site-directed mutagenesis, hydrophobic acyl-binding pocket is decreased in size by 1.75fold, 38fold reduced catalytic activity towards octanoyl-[acyl-carrier-protein] compared to the activity towards hexanoy-[acyl-carrier-protein]	486936
additional information	Trypanosoma brucei	Q586W9	construction of FAS or ELO enzyme-deficient mutant strains by RNA interference or gene disruption, ACP depletion by RNAi or gene knockout reduces lipoic acid levels and drastically decreases protein lipoylation	674882

Renatured/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

APPLICATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
drug development	Homo sapiens	Q9NWU1	the enzyme is a target for antibacterial drugs	677030
pharmacology	Mycobacterium tuberculosis	P63454	involved in biosynthesis of cell wall mycolic acids, therefor target for discovery of anti-tuberculosis agents	486942
drug development	Plasmodium falciparum	-	the enzyme is a target for antibacterial drugs	674777
drug development	Staphylococcus aureus	-	the enzyme is a target for antibacterial drugs	676903
pharmacology	Staphylococcus aureus	P99159	synthase III is a target for drug development against multi-drug resistant strains	486946
medicine	Synechocystis sp.	-	target for the development of drugs for the treatment of cancer and tuberculosis	486936, 486940
nutrition	Synechocystis sp.	-	target for the engineering of plant seed oils	486936, 486940
pharmacology	Synechocystis sp.	-	target for the development of drugs for the treatment of cancer and tuberculosis, involved in biosynthesis of precursors of pharmacological agents	486936, 486940

DISEASE	TITLE OF PUBLICATION	LINK TO PUBMED
Breast Neoplasms	Triclosan inhibits enoyl-reductase of type I fatty acid synthase in vitro and is cytotoxic to MCF-7 and SKBr-3 breast cancer cells.	PubMed
Myocardial Infarction	Condensing enzyme levels in the serum after experimental myocardial infarction.	PubMed
Neoplasms	Application of a flexible synthesis of (5R)-thiolactomycin to develop new inhibitors of type I fatty acid synthase.	PubMed
Tuberculosis	A novel interaction linking the FAS-II and phthiocerol dimycocerosate (PDIM) biosynthetic pathways.	PubMed
Tuberculosis	Acetylene-based analogues of thiolactomycin, active against Mycobacterium tuberculosis mtFabH fatty acid condensing enzyme.	PubMed
Tuberculosis	Biphenyl-based analogues of thiolactomycin, active against Mycobacterium tuberculosis mtFabH fatty acid condensing enzyme.	PubMed
Tuberculosis	Identification of 2-Aminothiazole-4-Carboxylate Derivatives Active against Mycobacterium tuberculosis H(37)R(v) and the beta-Ketoacyl-ACP Synthase mtFabH.	PubMed
Tuberculosis	Inhibition of a Mycobacterium tuberculosis beta-ketoacyl ACP synthase by isoniazid.	PubMed
Tuberculosis	Mycolic acid biosynthesis and enzymic characterization of the beta-ketoacyl-ACP synthase A-condensing enzyme from Mycobacterium tuberculosis.	PubMed
Tuberculosis	Purification and biochemical characterization of the Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthases KasA and KasB.	PubMed
Tuberculosis	X-ray crystal structure of Mycobacterium tuberculosis beta-ketoacyl acyl carrier protein synthase II (mtKasB).	PubMed

REF.	AUTHORS	TITLE	JOURNAL	VOL.	PAGES	YEAR	ORGANISM	LINK TO PUBMED	SOURCE
285669	Shimakata, T.; Stumpf, P.K.	The procaryotic nature of the fatty acid synthetase of developing Carthamus tinctorius L. (Safflower) seeds	Arch. Biochem. Biophys.	217	144-154	1982	Carthamus tinctorius	PubMed
285675	Shimakata, T.; Stumpf, P.K.	The purification and function of acetyl coenzyme A:acyl carrier protein transacylase	J. Biol. Chem.	258	3592-3598	1983	Brassica juncea, Carthamus tinctorius, Cuphea lutea, Pisum sativum, Spinacia oleracea	PubMed
285676	Hendren, R.W.; Bloch, K.	Fatty acid synthetases from Euglena gracilis. Separation of component activities of the ACP-dependent fatty acid synthetase and partial purification of the beta-ketoacyl-ACP synthetase	J. Biol. Chem.	255	1504-1508	1980	Euglena gracilis	PubMed
486863	Vagelos, R.P.	Acyl group transfer (acyl carrier protein)	The Enzymes, 3rd Ed. (Boyer, P.D., ed.)	8	155-199	1973	Clostridium kluyveri, Escherichia coli	-
486864	Prescott, D.J.; Vagelos, P.R.	Acyl carrier protein	Adv. Enzymol. Relat. Areas Mol. Biol.	36	269-311	1972	Escherichia coli	PubMed
486914	Toomey, R.E.; Wakil, S.J.	Studies on the mechanism of fatty acid synthesis. XVI. Preparation and general properties of acyl-malonyl acyl carrier protein-condensing enzyme from Escherichia coli	J. Biol. Chem.	241	1159-1165	1966	Escherichia coli	PubMed
486915	Alberts, A.W.; Majerus, P.W.; Vagelos, P.R.	beta-Ketoacyl acyl carrier protein synthase	Methods Enzymol.	14	57-60	1969	Escherichia coli	-
486916	Garwin, J.L.; Klages, A.L.; Cronan, J.E.	Structural, enzymatic, and genetic studies of beta-ketoacyl-acyl carrier protein synthases I and II of Escherichia coli	J. Biol. Chem.	255	11949-11956	1980	Escherichia coli	PubMed
486917	Alberts, A.W.; Bell, R.M.; Vagelos, P.R.	Acyl carrier protein. XV. Studies of beta-ketoacyl-acyl carrier protein synthetase	J. Biol. Chem.	247	3190-3198	1972	Escherichia coli	PubMed
486918	D'Agnolo, G.; Rosenfeld, I.S.; Vagelos, P.R.	beta-Ketoacyl-acyl carrier protein synthetase. Characterization of the acyl-enzyme intermediate	J. Biol. Chem.	250	5283-5288	1975	Escherichia coli	PubMed
486919	D'Agnolo, G.; Rosenfeld, I.S.; Vagelos, P.R.	Multiple forms of beta-ketoacyl-acyl carrier protein synthetase in Escherichia coli	J. Biol. Chem.	250	5289-5294	1975	Escherichia coli	PubMed
486920	Shimakata, T.; Stumpf, P.L.	Purification and characterization of beta-ketoacyl-[acyl-carrier-protein] synthetase I from Spinacia oleracea leaves	Arch. Biochem. Biophys.	220	39-45	1983	Spinacia oleracea	PubMed
486921	Schuz, R.; Ebel, J.; Hahlbrock, K.	Partial purification of beta-ketoacyl-acyl acrrier protein synthase from a higher plant	FEBS Lett.	140	207-209	1982	Petroselinum crispum	-
486922	MacKintosh, R.W.; Hardie, D.G.; Slabas, A.R.	beta-Ketoacyl-acyl-carrier protein synthase from developing seeds of oilseed rape (Brassica napus)	Biochem. Soc. Trans.	17	686-687	1989	Brassica napus	-
486923	MacKintosh, R.W.; Hardie, D.G.; Slabas, A.R.	A new assay procedure to study the induction of beta-ketoacyl-ACP synthase I and II, and the complete purification of beta-ketoacyl-ACP synthase I from developing seeds of oilseed rape (Brassica napus)	Biochim. Biophys. Acta	1002	114-124	1989	Brassica napus	-
486924	Shimakata, T.; Stumpf, P.L.	Isolation and function of spinach leaf beta-ketoacyl-[acyl-carrier-protein] synthases	Proc. Natl. Acad. Sci. USA	79	5808-5812	1982	Spinacia oleracea	PubMed
486925	Siggaard-Andersen, M.; Kauppinen, S.; von Wettstein-Knowles, P.	Primary structure of a cerulenin-binding beta-ketoacyl-[acyl carrier protein] synthase from barley chloroplasts	Proc. Natl. Acad. Sci. USA	88	4114-4118	1991	Hordeum vulgare, Spinacia oleracea	PubMed
486931	Clough, R.; Matthis, A.L.; Barnum, S.R.; Jaworski, J.G.	Purification and characterization of 3-ketoacyl-acyl carrier protein synthase III from spinach. A condensing enzyme utilizing acetyl-coenzyme A to initiate fatty acid synthesis	J. Biol. Chem.	267	20992-20998	1992	Spinacia oleracea	PubMed
486932	Verwoert, I.I.G.S.; van der Linden, K.H.; Walsh, M.C.; Nijkamp, H.J.J.; Stuitje, A.R.	Modification of Brassica napus seed oil by expression of the Escherichia coli fabH gene, encoding 3-ketoacyl-acyl carrier protein synthase III	Plant Mol. Biol.	27	875-886	1995	Brassica napus, Escherichia coli	PubMed
486933	Han, L.; Lobo, S.; Reynolds, K.A.	Characterization of beta-ketoacyl-acyl carrier protein synthase III from Streptomyces glaucescens and its role in initiation of fatty acid biosynthesis	J. Bacteriol.	180	4481-4486	1998	Streptomyces glaucescens	PubMed
486934	Bao, W.; Sheldon, P.J.; Hutchinson, C.R.	Purification and properties of the Streptomyces peucetius DpsC beta-ketoacyl:acyl carrier protein synthase III that specifies the propionate-starter unit for type II polyketide biosynthesis	Biochemistry	38	9752-9757	1999	Streptomyces peucetius	PubMed
486935	Domergue, F.; Post-Beittenmiller, D.	Biochemical characterization of 3-ketoacyl-acyl carrier protein synthase II from leek epidermis	Biochem. Soc. Trans.	28	610-613	2000	Allium ampeloprasum	PubMed
486936	Val, D.; Banu, G.; Seshadri, K.; Lindqvist, Y.; Dehesh, K.	Re-engineering ketoacyl synthase specificity	Structure	8	565-566	2000	Synechocystis sp.	PubMed
486937	Choi, K.H.; Heath, R.J.; Rock, C.O.	beta-ketoacyl-acyl carrier protein synthase III (FabH) is a determining factor in branched-chain fatty acid biosynthesis	J. Bacteriol.	182	365-370	2000	Bacillus subtilis, Escherichia coli	PubMed
486938	Smirnova, N.; Reynolds, K.A.	Engineered fatty acid biosynthesis in Streptomyces by altered catalytic function of beta-ketoacyl-acyl carrier protein synthase III	J. Bacteriol.	183	2335-2342	2001	Streptomyces glaucescens	PubMed
486939	Khandekar, S.S.; Gentry, D.R.; Van Aller, G.S.; Warren, P.; Xiang, H.; Silverman, C.; Doyle, M.L.; Chambers, P.A.; Konstantinidis, A.K.; Brandt, M.; Daines, R.A.; Lonsdale, J.T.	Identification, substrate specificity, and inhibition of the Streptococcus pneumoniae beta-ketoacyl-acyl carrier protein synthase III (FabH)	J. Biol. Chem.	276	30024-30030	2001	Escherichia coli, Haemophilus influenzae, Streptococcus pneumoniae	PubMed
486940	Moche, M.; Dehesh, K.; Edwards, P.; Lindqvist, Y.	The crystal structure of beta-ketoacyl-acyl carrier protein synthase II from Synechocystis sp. at 1.54.ANG. resolution and its relationship to other condensing enzymes	J. Mol. Biol.	305	491-503	2001	Synechocystis sp.	PubMed
486941	Price, A.C.; Choi, K.H.; Heath, R.J.; Li, Z.; White, S.W.; Rock, C.O.	Inhibition of beta-ketoacyl-acyl carrier protein synthases by thiolactomycin and cerulenin. Structure and mechanism	J. Biol. Chem.	276	6551-6559	2001	Escherichia coli	PubMed
486942	Schaeffer, M.L.; Agnihotri, G.; Volker, C.; Kallender, H.; Brennan, P.J.; Lonsdale, J.T.	Purification and biochemical characterization of the Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthases KasA and KasB	J. Biol. Chem.	276	47029-47037	2001	Mycobacterium tuberculosis	PubMed
486943	McGuire, K.A.; Siggaard-Andersen, M.; Bangera, M.G.; Olsen, J.G.; von Wettstein-Knowles, P.	beta-Ketoacyl-[acyl carrier protein] synthase I of Escherichia coli: aspects of the condensation mechanism revealed by analyses of mutations in the active site pocket	Biochemistry	40	9836-9845	2001	Escherichia coli	PubMed
486944	Witkowski, A.; Joshi, A.K.; Smith, S.	Mechanism of the beta-ketoacyl synthase reaction catalyzed by the animal fatty acid synthase	Biochemistry	41	10877-10887	2002	Rattus norvegicus	PubMed
486946	He, X.; Reynolds, K.A.	Purification, characterization, and identification of novel inhibitors of the beta-ketoacyl-acyl carrier protein synthase III (FabH) from Staphylococcus aureus	Antimicrob. Agents Chemother.	46	1310-1318	2002	Staphylococcus aureus	PubMed
486947	Schutt, B.S.; Abbadi, A.; Loddenkotter, B.; Brummel, M.; Spener, F.	beta-Ketoacyl-acyl carrier protein synthase IV: a key enzyme for regulation of medium-chain fatty acid synthesis in Cuphea lanceolata seeds	Planta	215	847-854	2002	Cuphea lanceolata	PubMed
486948	Mekhedov, S.; Cahoon, E.B.; Ohlrogge, J.	An unusual seed-specific 3-ketoacyl-ACP synthase associated with the biosynthesis of petroselinic acid in coriander	Plant Mol. Biol.	47	507-518	2001	Coriandrum sativum	PubMed
658746	Olsen, J.G.; Rasmussen, A.V.; von Wettstein-Knowles, P.; Henriksen, A.	Structure of the mitochondrial beta-ketoacyl-[acyl carrier protein] synthase from Arabidopsis and its role in fatty acid synthesis	FEBS Lett.	577	170-174	2004	Arabidopsis thaliana	PubMed
659029	Price, A.C.; Rock, C.O.; White, S.W.	The 1.3-Angstrom-resolution crystal structure of beta-ketoacyl-acyl carrier protein synthase II from Streptococcus pneumoniae	J. Bacteriol.	185	4136-4143	2003	Streptococcus pneumoniae	PubMed
659294	Lai, C.Y.; Cronan, J.E.	beta-Ketoacyl-acyl carrier protein synthase III (FabH) is essential for bacterial fatty acid synthesis	J. Biol. Chem.	278	51494-51503	2003	Lactococcus lactis	PubMed
659442	Yasuno, R.; von Wettstein-Knowles, P.; Wada, H.	Identification and molecular characterization of the beta-ketoacyl-[acyl carrier protein] synthase component of the Arabidopsis mitochondrial fatty acid synthase	J. Biol. Chem.	279	8242-8251	2004	Arabidopsis thaliana	PubMed
659451	Zhang, L.; Joshi, A.K.; Hofmann, J.; Schweizer, E.; Smith, S.	Cloning, expression, and characterization of the human mitochondrial beta-ketoacyl synthase. Complementation of the yeast CEM1 knock-out strain	J. Biol. Chem.	280	12422-12429	2005	Homo sapiens	PubMed
659493	Brown, A.K.; Sridharan, S.; Kremer, L.; Lindenberg, S.; Dover, L.G.; Sacchettini, J.C.; Besra, G.S.	Probing the mechanism of the Mycobacterium tuberculosis beta-ketoacyl-ACP synthase III mtFabH: Factors influencing catalysis and substrate specificity	J. Biol. Chem.	280	32539-32547	2005	Mycobacterium tuberculosis	PubMed
659724	Musayev, F.; Sachdeva, S.; Scarsdale, J.N.; Reynolds, K.A.; Wright, H.T.	Crystal structure of a substrate complex of Mycobacterium tuberculosis beta-ketoacyl-acyl carrier protein synthase III (FabH) with lauroyl-coenzyme A	J. Mol. Biol.	346	1313-1321	2005	Mycobacterium tuberculosis	PubMed
659726	Kursula, P.; Sikkilae, H.; Fukao, T.; Kondo, N.; Wierenga, R.K.	High resolution crystal structures of human cytosolic thiolase (CT): A comparison of the active sites of human CT, bacterial thiolase, and bacterial KAS I	J. Mol. Biol.	347	189-201	2005	Escherichia coli	PubMed
660466	Qiu, X.; Choudhry, A.E.; Janson, C.A.; Grooms, M.; Daines, R.A.; Lonsdale, J.T.; Khandekar, S.S.	Crystal structure and substrate specificity of the beta-ketoacyl-acyl carrier protein synthase III (FabH) from Staphylococcus aureus	Protein Sci.	14	2087-2094	2005	Staphylococcus aureus	PubMed
660470	Dawe, J.H.; Porter, C.T.; Thornton, J.M.; Tabor, A.B.	A template search reveals mechanistic similarities and differences in beta-ketoacyl synthases (KAS) and related enzymes	Proteins	52	427-435	2003	Escherichia coli	PubMed
661000	Rendina, A.R.; Cheng, D.	Characterization of the inactivation of rat fatty acid synthase by C75: inhibition of partial reactions and protection by substrates	Biochem. J.	388	895-903	2005	Rattus norvegicus	PubMed
673576	von Wettstein-Knowles, P.; Olsen, J.G.; McGuire, K.A.; Henriksen, A.	Fatty acid synthesis. Role of active site histidines and lysine in Cys-His-His-type beta-ketoacyl-acyl carrier protein synthases	FEBS J.	273	695-710	2006	Escherichia coli	PubMed
674095	Salas, J.J.; Martinez-Force, E.; Garces, R.	Very long chain fatty acid synthesis in sunflower kernels	J. Agric. Food Chem.	53	2710-2716	2005	Helianthus annuus	PubMed
674777	Lack, G.; Homberger-Zizzari, E.; Folkers, G.; Scapozza, L.; Perozzo, R.	Recombinant expression and biochemical characterization of the unique elongating beta-ketoacyl-acyl carrier protein synthase involved in fatty acid biosynthesis of Plasmodium falciparum using natural and artificial substrates	J. Biol. Chem.	281	9538-9546	2006	Plasmodium falciparum	PubMed
674882	Stephens, J.L.; Lee, S.H.; Paul, K.S.; Englund, P.T.	Mitochondrial fatty acid synthesis in Trypanosoma brucei	J. Biol. Chem.	282	4427-4436	2007	Trypanosoma brucei	PubMed
676903	Wang, J.; Kodali, S.; Lee, S.H.; Galgoci, A.; Painter, R.; Dorso, K.; Racine, F.; Motyl, M.; Hernandez, L.; Tinney, E.; Colletti, S.L.; Herath, K.; Cummings, R.; Salazar, O.; Gonzalez, I.; Basilio, A.; Vicente, F.; Genilloud, O.; Pelaez, F.; Jayasuriya, H.; Young, K.; Cully, D.F.; Singh, S.B.	Discovery of platencin, a dual FabF and FabH inhibitor with in vivo antibiotic properties	Proc. Natl. Acad. Sci. USA	104	7612-7616	2007	Staphylococcus aureus	PubMed
677030	Christensen, C.E.; Kragelund, B.B.; von Wettstein-Knowles, P.; Henriksen, A.	Structure of the human beta-ketoacyl [ACP] synthase from the mitochondrial type II fatty acid synthase	Protein Sci.	16	261-272	2007	Homo sapiens	PubMed
704553	Feng, Y.; Cronan, J.E.	Escherichia coli unsaturated fatty acid synthesis: complex transcription of the fabA gene and in vivo identification of the essential reaction catalyzed by FabB	J. Biol. Chem.	284	29526-29535	2009	Escherichia coli	PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 2.3.1.41)

NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM

IUBMB Enzyme Nomenclature

PROSITE Database of protein families and domains

SYSTERS

Protein Mutant Database

InterPro (database of protein families, domains and functional sites)

All organisms
Allium ampeloprasum
Arabidopsis thaliana
Bacillus subtilis
Brassica juncea
Brassica napus
Carthamus tinctorius
Clostridium kluyveri
Coriandrum sativum
Cuphea lanceolata
Cuphea lutea
Escherichia coli
Euglena gracilis
Haemophilus influenzae
Helianthus annuus
Homo sapiens
Hordeum vulgare
Lactococcus lactis
Mycobacterium tuberculosis
Petroselinum crispum
Pisum sativum
Plasmodium falciparum
Rattus norvegicus
Spinacia oleracea
Staphylococcus aureus
Streptococcus pneumoniae
Streptomyces glaucescens
Streptomyces peucetius
Synechocystis sp.
Trypanosoma brucei