Information on EC 2.1.2.3 - phosphoribosylaminoimidazolecarboxamide formyltransferase:
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EC NUMBERCOMMENTARY
2.1.2.3-

RECOMMENDED NAMEGeneOntology No.
phosphoribosylaminoimidazolecarboxamide formyltransferaseGO:0004643

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
show the reaction diagram		----
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide = tetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
show the reaction diagram		mechanismHomo sapiens-658026

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
formyl group transfer----
formyl group transferHomo sapiens--673127
formyl group transferThermotoga maritima--689976

PATHWAYKEGG LinkMetaCyc Link
inosine-5'-phosphate biosynthesis I-PWY-6123
inosine-5'-phosphate biosynthesis II-PWY-6124

SYSTEMATIC NAMEIUBMB Comments
10-formyltetrahydrofolate:5'-phosphoribosyl-5-amino-4-imidazole-carboxamide N-formyltransferase-

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
10-formyltetrahydrofolate:5'-phosphoribosyl-5-amino-4-imidazolecarboxamide formyltransferase----
5'-phosphoribosyl-5-amino-4-imidazolecarboxamide formyltransferase----
5-amino-1-ribosyl-4-imidazolecarboxamide 5'-phosphate transformylase----
5-amino-4-imidazolecarboxamide ribonucleotide transformylase----
5-amino-4-imidazolecarboxamide ribotide formyltransferaseHomo sapiens--702155
5-amino-4-imidazolecarboxamide ribotide transformylase----
5-aminoimidazole-4-carboxamide ribonucleotide transformylaseHomo sapiens--673127
5-aminoimidazole-4-carboxamide ribonucleotide transformylaseThermotoga maritima--689976
5-aminoimidazole-4-carboxamide ribonucleotide transformylase (AICAR Tfase)-inosine monophosphate cyclohydrolaseGallus sp.--485755
5-aminoimidazole-4-carboxamide ribonucleotide transformylase/inosine 5'-monophosphate cyclohydrolaseHomo sapiens--485758
5-aminoimidazole-4-carboxamide ribotide transformylase-IMP cyclohydrolaseHomo sapiens-bifunctional enzyme172161, 172163
AICA-ribotide formyltransferasePisum sativum--485745
AICAR formyltransferase----
AICAR formyltransferaseHomo sapiens--702155
AICAR TFaseGallus sp.--485747
AICAR TFaseThermotoga maritima--689976
AICAR transformylase----
AICAR transformylaseHomo sapiens--673127
AICAR transformylaseThermotoga maritima--689976
AICAR transformylase/inosine monophosphate cyclohydrolaseHomo sapiens-bifunctional enzyme catalyzing the last two steps of the purine de novo synthesis pathway652530, 658026, 658284, 659419, 659681
AICAR transformylase/inosine monophosphate cyclohydrolaseGallus gallusP31335bifunctional enzyme catalyzing the last two steps of the purine de novo synthesis pathway657946, 659419
AICAR transformylase/inosine monophosphate cyclohydrolaseaves-bifunctional enzyme catalyzing the last two steps of the purine de novo synthesis pathway658026
AICARFTHomo sapiens--172163, 485759, 702155
AICARFT/IMPCHaseHomo sapiensP31939-172163
aminoimidazolecarboxamide ribonucleotide transformylase----
ATICGallus sp.--485755, 485758, 485759
ATICHomo sapiens--485758, 485759, 658026
ATICaves--658026
IMPCHGallus sp.--485755
PurHThermotoga maritima--689976
TM1249Thermotoga maritima--689976

CAS REGISTRY NUMBERCOMMENTARY
9032-03-5-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Allium cepaonion485745--Manually annotated by BRENDA team
aves-658026--Manually annotated by BRENDA team
Bacillus subtilis-172161, 172163, 485754, 485757--Manually annotated by BRENDA team
Columba sp.pigeon485745--Manually annotated by BRENDA team
Daucus carotacarrot485745--Manually annotated by BRENDA team
Escherichia coli-172161, 172163, 485745, 485756, 485757--Manually annotated by BRENDA team
Escherichia coliwild-type strain 5928, strain PA 15485744--Manually annotated by BRENDA team
Gallus gallus-657946, 659419P31335UniProtManually annotated by BRENDA team
Gallus sp.-485753, 485757--Manually annotated by BRENDA team
Gallus sp.avian172163, 485755, 485758, 485759, 485760--Manually annotated by BRENDA team
Gallus sp.avian; chicken172161--Manually annotated by BRENDA team
Gallus sp.chicken485742, 485745, 485746, 485747, 485748, 485749, 485750--Manually annotated by BRENDA team
Homo sapiens-652530, 659419, 659681P31939SwissProtManually annotated by BRENDA team
Homo sapiens-658026, 658284, 673127, 702155, 705032--Manually annotated by BRENDA team
Homo sapiensbifunctional 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase696946--Manually annotated by BRENDA team
Homo sapienshuman172161, 485750, 485754, 485756, 485757, 485758, 485759, 485760--Manually annotated by BRENDA team
Homo sapienshuman172163P31939SwissProtManually annotated by BRENDA team
Mus musculus-485747, 485749--Manually annotated by BRENDA team
Mus musculusmouse, strain MRL/lpr485750--Manually annotated by BRENDA team
Petroselinum crispumparsley485745--Manually annotated by BRENDA team
Pisum sativumpea, L. var. Alaska485745--Manually annotated by BRENDA team
Saccharomyces cerevisiaeyeast485758--Manually annotated by BRENDA team
Salmonella enterica subsp. enterica serovar Typhimurium-172163, 485754, 485757--Manually annotated by BRENDA team
Spinacia oleraceaspinach485745--Manually annotated by BRENDA team
Thermotoga maritima-689976--Manually annotated by BRENDA team
Trifolium sp.trefoil485745--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
(6R,6S)-10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Homo sapiensP31939-172163-172163r
10-formyl-7,8-dihydropteroylpenta-gamma-glutamate + 5'-phosphoribosyl-5-amino-4-imidazolecarboxamide7,8-dihydropteroylpenta-gamma-glutarate + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Gallus sp.--485749-485749?
10-formyl-8-deazafolate + 5'-phosphoribosyl-5-amino-4-imidazolecarboxamide8-deazafolate + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Gallus sp.--485748-485748?
10-formyl-tetrahydrofolic acid + 5'-phosphoribosyl-5-amino-imidazole-carboxamide5,6,7,8-tetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram		Homo sapiens--702155--?
10-formyldihydrofolic acid pentaglutamate + 5'-phosphoribosyl-5-amino-4-imidazolecarboxamidedihydrofolic acid pentaglutamate + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Gallus sp.--485749-485749?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram		Homo sapiens--658026, 702155--?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram		Homo sapiens-penultimate step of the purine de novo synthesis pathway658026--?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamidetetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram		Gallus gallusP31335-657946--?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamidetetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram		Gallus gallus--659419--?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamidetetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram		Homo sapiensP31939-652530--?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamidetetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram		Homo sapiens--658284, 659419, 659681--?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamidetetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram		aves--658026--?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamidetetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram		Gallus gallusP31335penultimate step of the purine de novo synthesis pathway657946--?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamidetetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram		Gallus gallus-penultimate step of the purine de novo synthesis pathway659419--?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamidetetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram		Homo sapiensP31939penultimate step of the purine de novo synthesis pathway652530--?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamidetetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram		Homo sapiens-penultimate step of the purine de novo synthesis pathway658284, 659419, 659681--?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamidetetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram		aves-penultimate step of the purine de novo synthesis pathway658026--?
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Salmonella enterica subsp. enterica serovar Typhimurium--172163, 485754, 485757--r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Bacillus subtilis--172161, 172163, 485754, 485757--r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Mus musculus--485747, 485749, 485750--r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Escherichia coli--172161, 172163, 485744, 485745, 485756, 485757--r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Homo sapiens--485750, 485756--r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Saccharomyces cerevisiae--485758--r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Spinacia oleracea, Petroselinum crispum, Daucus carota, Allium cepa--485745--r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Gallus sp.--485746, 485747, 485749, 485750--r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Columba sp., Trifolium sp.--485745--r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Gallus sp.-precursors for inosinic acid biosynthesis485742--r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Homo sapiensP31939penultimate and final steps in the de novo purine biosynthesis pathway172163--r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Homo sapiens-penultimate and final steps in the de novo purine biosynthesis pathway485754, 485757, 485758, 485759, 485760--r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Gallus sp.-penultimate and final steps in the de novo purine biosynthesis pathway172163, 485755, 485757, 485758, 485759, 485760--r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Homo sapiens-de novo synthesis of purine nucleotides172161, 485757, 485758--r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Pisum sativum-de novo synthesis of purine nucleotides485745--r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Gallus sp.-de novo synthesis of purine nucleotides172161, 485745, 485753, 485757, 485758--r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Homo sapiens-reduced folate cofactor requiring step of de novo purine biosynthesis485759--r
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Gallus sp.-reduced folate cofactor requiring step of de novo purine biosynthesis485748, 485759--r
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamidetetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
show the reaction diagram		Homo sapiens--673127--?
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamidetetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
show the reaction diagram		Thermotoga maritima--689976--?
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Salmonella enterica subsp. enterica serovar Typhimurium--172163-172163r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Salmonella enterica subsp. enterica serovar Typhimurium--485754-485754r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Salmonella enterica subsp. enterica serovar Typhimurium--485757-485757r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Bacillus subtilis--172161-172161r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Bacillus subtilis--172163-172163r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Bacillus subtilis--485754-485754r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Bacillus subtilis--485757-485757r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Mus musculus--485747-485747r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Mus musculus--485749-485749r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Mus musculus--485750-485750r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Escherichia coli--172161-172161r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Escherichia coli--172163-172163r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Escherichia coli--485744-485744r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Escherichia coli--485745-485745r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Escherichia coli--485756-485756r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Escherichia coli--485757-485757r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Homo sapiens--172161-172161r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Homo sapiensP31939-172163-172163r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Homo sapiens--485750-485750r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Homo sapiens--485754-485754r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Homo sapiens--485756-485756r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Homo sapiens--485757-485757r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Homo sapiens--485758-485758r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Homo sapiens--485759-485759r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Homo sapiens--485760-485760r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Saccharomyces cerevisiae--485758-485758r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Spinacia oleracea, Pisum sativum, Petroselinum crispum, Daucus carota, Allium cepa--485745-485745r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Gallus sp.--172161-172161r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Gallus sp.--172163-172163r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Gallus sp.--485742-485742r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Gallus sp.--485745-485745r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Gallus sp.--485746-485746r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Gallus sp.--485747-485747r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Gallus sp.--485748-485748r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Gallus sp.--485749-485749r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Gallus sp.--485750-485750r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Gallus sp.--485753-485753r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Gallus sp.--485755-485755r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Gallus sp.--485757-485757r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Gallus sp.--485758-485758r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Gallus sp.--485759-485759r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Gallus sp.--485760-485760r
N10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotidetetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Columba sp., Trifolium sp.--485745-485745r
N10-formyltetrahydropteroylglutamate + 5'-phosphoribosyl-5-formamido-4-imidazole carboxamide ribonucleotidetetrahydropteroylglutamate + 5'-phosphoribosyl-5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Gallus sp.--485746-485746?
N10-formyltetrahydropteroylglutamate + 5'-phosphoribosyl-5-formamido-4-imidazole carboxamide ribonucleotidetetrahydropteroylglutamate + 5'-phosphoribosyl-5-formamido-4-imidazole carboxamide
show the reaction diagram		Gallus sp.--485746--?
N10-formyltetrahydropteroylglutamate + 5'-phosphoribosyl-5-formamino-4-imidazole carboxamide ribonucleotidetetrahydropteroylglutamate + 5'-phosphoribosyl-5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Gallus sp.--485746-485746?
N10-formyltetrahydropteroylheptaglutamate + 5'-phosphoribosyl-5-formamino-4-imidazole carboxamide ribonucleotidetetrahydropteroylglutamate + 5'-phosphoribosyl-5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Gallus sp.--485746-485746?
N10-formyltetrahydropteroylhexaglutamate + 5'-phosphoribosyl-5-formamino-4-imidazole carboxamide ribonucleotidetetrahydropteroylglutamate + 5'-phosphoribosyl-5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Gallus sp.--485746-485746?
N10-formyltetrahydropteroylmonoglutamate + 5'-phosphoribosyl-5-formamino-4-imidazole carboxamide ribonucleotidetetrahydropteroylmonoglutamate + 5'-phosphoribosyl-5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Gallus sp.--485746-485746?
N10-formyltetrahydropteroylpentaglutamate + 5'-phosphoribosyl-5-formamino-4-imidazole carboxamide ribonucleotidetetrahydropteroylglutamate + 5'-phosphoribosyl-5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Gallus sp.--485746-485746?
N10-formyltetrahydropteroyltetraglutamate + 5'-phosphoribosyl-5-formamino-4-imidazole carboxamide ribonucleotidetetrahydropteroylglutamate + 5'-phosphoribosyl-5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Gallus sp.--485746-485746?
N10-formyltetrahydropteroyltriglutamate + 5'-phosphoribosyl-5-formamino-4-imidazole carboxamide ribonucleotidetetrahydorpteroylglutamate + 5'-phosphoribosyl-5-formamido-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Gallus sp.--485746-485746?
N5,N10-anhydroformyltetrahydrofolic acid + glycinamide ribonucleotideN10-formyltetrahydrofolic acid + formylglycinamide ribonucleotide
show the reaction diagram		Gallus sp.--485742-485742r
tetrahydrofolic acid + 5-formamido-4-imidazole carboxamide ribonucleotideN10-formyltetrahydrofolic acid + 5-amino-4-imidazole carboxamide ribonucleotide
show the reaction diagram		Gallus sp.--485742-485742r
10-formyltetrahydrofolate + 5-amino-imidazole-4-thiocarboxamide ribonucleotidetetrahydrofolate + 6-mercaptopurine ribonucleotide
show the reaction diagram		Homo sapiens--485756-485756?
additional information?-Escherichia coli-no activity with N5,N10-methenyl analogues485744---
additional information?-Salmonella enterica subsp. enterica serovar Typhimurium-in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene172163, 485754, 485757---
additional information?-Bacillus subtilis-in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene172161, 172163, 485754, 485757---
additional information?-Mus musculus-in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene485747, 485749, 485750---
additional information?-Escherichia coli-in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene172161, 172163, 485744, 485745, 485756, 485757---
additional information?-Homo sapiens-in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene172161---
additional information?-Homo sapiensP31939in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene172163---
additional information?-Homo sapiens-in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene485750, 485754, 485756, 485757, 485758, 485759, 485760---
additional information?-Saccharomyces cerevisiae-in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene485758---
additional information?-Spinacia oleracea, Pisum sativum, Petroselinum crispum, Daucus carota, Allium cepa-in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene485745---
additional information?-Gallus sp.-in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene172161, 172163, 485742, 485745, 485746, 485747, 485748, 485749, 485750, 485753, 485755, 485757, 485758, 485759, 485760---
additional information?-Columba sp., Trifolium sp.-in all organisms studied to date AICARFT activity is accompanied by inosine monophosphate cyclohydrolase located on the same polypeptide encoded by the purH gene485745---
additional information?-Homo sapiens-polymorphisms in 5-aminoimidazole-4-carboxamide ribonucleotide transformylase gene is not associated with recurrent venous thrombosis risk673127---

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram		Homo sapiens-penultimate step of the purine de novo synthesis pathway658026--
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamidetetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram		Gallus gallusP31335penultimate step of the purine de novo synthesis pathway657946--
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamidetetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram		Gallus gallus-penultimate step of the purine de novo synthesis pathway659419--
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamidetetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram		Homo sapiensP31939penultimate step of the purine de novo synthesis pathway652530--
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamidetetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram		Homo sapiens-penultimate step of the purine de novo synthesis pathway658284, 659419, 659681--
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazole-4-carboxamidetetrahydrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazole-4-carboxamide
show the reaction diagram		aves-penultimate step of the purine de novo synthesis pathway658026--
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Salmonella enterica subsp. enterica serovar Typhimurium--172163, 485754, 485757--
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Bacillus subtilis--172161, 172163, 485754, 485757--
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Mus musculus--485747, 485749, 485750--
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Escherichia coli--172161, 172163, 485744, 485745, 485756, 485757--
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Homo sapiens--485750, 485756--
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Saccharomyces cerevisiae--485758--
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Spinacia oleracea, Petroselinum crispum, Daucus carota, Allium cepa--485745--
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Gallus sp.--485746, 485747, 485749, 485750--
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Columba sp., Trifolium sp.--485745--
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Gallus sp.-precursors for inosinic acid biosynthesis485742--
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Homo sapiensP31939penultimate and final steps in the de novo purine biosynthesis pathway172163--
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Homo sapiens-penultimate and final steps in the de novo purine biosynthesis pathway485754, 485757, 485758, 485759, 485760--
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Gallus sp.-penultimate and final steps in the de novo purine biosynthesis pathway172163, 485755, 485757, 485758, 485759, 485760--
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Homo sapiens-de novo synthesis of purine nucleotides172161, 485757, 485758--
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Pisum sativum-de novo synthesis of purine nucleotides485745--
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Gallus sp.-de novo synthesis of purine nucleotides172161, 485745, 485753, 485757, 485758--
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Homo sapiens-reduced folate cofactor requiring step of de novo purine biosynthesis485759--
10-formyltetrahydrofolate + 5'-phosphoribosyl-5-amino-imidazolecarboxamidetetrahdrofolic acid + 5'-phosphoribosyl-5-formamido-4-imidazolecarboxamide
show the reaction diagram		Gallus sp.-reduced folate cofactor requiring step of de novo purine biosynthesis485748, 485759--
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamidetetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
show the reaction diagram		Homo sapiens--673127--
N10-formyltetrahydropteroylglutamate + 5'-phosphoribosyl-5-formamido-4-imidazole carboxamide ribonucleotidetetrahydropteroylglutamate + 5'-phosphoribosyl-5-formamido-4-imidazole carboxamide
show the reaction diagram		Gallus sp.--485746--
10-formyltetrahydrofolate + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamidetetrahydrofolate + 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
show the reaction diagram		Thermotoga maritima--689976--
additional information?-Homo sapiens-polymorphisms in 5-aminoimidazole-4-carboxamide ribonucleotide transformylase gene is not associated with recurrent venous thrombosis risk673127--

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
10-formyldihydrofolateHomo sapiens--485756 2D-image
10-formylfolateHomo sapiens--485756 2D-image
10-formyltetrahydrofolateHomo sapiens--652530, 658026, 658284, 659419, 659681, 673127 2D-image
10-formyltetrahydrofolateGallus gallusP31335-657946, 659419 2D-image
10-formyltetrahydrofolateaves--658026 2D-image
N10-formyltetrahydrofolateGallus sp.--485745, 485747, 485759, 485760 2D-image
N10-formyltetrahydrofolatePisum sativum--485745 2D-image
N10-formyltetrahydrofolateHomo sapiens--485756, 485759, 485760 2D-image
10-formyltetrahydrofolateThermotoga maritima--689976 2D-image
additional informationaves-contains selenomethionine658026-

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
K+Gallus sp.--485745
K+Pisum sativum-essential component485745
K+Gallus sp.-required for maximum enzymatic activity485746
K+Thermotoga maritima-the refined structure includes two K+ ions in the asymmetric unit689976

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
(2RS)-N-[4-{1-(acetamideoxy)-3-(2-amino-3,4-dihydro-4-oxo-quinazolin-6-yl)prop-2-yl}benzoyl]-L-glutamic acidGallus sp.--485753 2D-image
(2RS)-N-[4-{1-(acetoxy)-3-(2-amino-3,4-dihydro-4-oxo-quinazolin-6-yl)prop-2-yl}benzoyl]-L-glutamic acidGallus sp.--485753 2D-image
(2RS)-N-[4-{3-(2-amino-3,4-dihydro-4-oxo-quinazolin-6-yl)-1,1-(dimethoxy)prop-2-yl}benzoyl]-L-glutamic acidGallus sp.--485753 2D-image
(2RS)-N-[4-{3-(2-amino-3,4-dihydro-4-oxo-quinazolin-6-yl)-1-(1,1-bromoacetoxy)prop-2-yl}benzoyl]-L-glutamic acidGallus sp.--485753 2D-image
(2RS)-N-[4-{3-(2-amino-3,4-dihydro-4-oxo-quinazolin-6-yl)-1-(1,1-dimethoxyacetoxy)prop-2-yl}benzoyl]-L-glutamic acidGallus sp.--485753 2D-image
(2RS)-N-[4-{3-(2-amino-3,4-dihydro-4-oxo-quinazolin-6-yl)-1-(ethoxalyoxy) prop-2-yl}benzoyl]-L-glutamic acidGallus sp.--485753 2D-image
(2RS)-N-[4-{3-(2-amino-3,4-dihydro-4-oxo-quinazolin-6-yl)-1-(formyloxy)prop-2-yl}benzoyl]-L-glutamic acidGallus sp.--485753 2D-image
(2RS)-N-[4-{3-(2-amino-3,4-dihydro-4-oxo-quinazolin-6-yl)-1-(hydroxy)prop-2-yl}benzoyl]-L-glutamic acidGallus sp.--485753 2D-image
(2RS)-N-[4-{3-(2-amino-3,4-dihydro-4-oxo-quinazolin-6-yl)-1-(pyruvoyloxy)prop-2-yl}benzoyl]-L-glutamic acidGallus sp.--485753 2D-image
(6S/R)-5,10-dideaza-5,6,7,8,-tetrahydrofolateHomo sapiens--485758, 485759 2D-image
10-formyl-5,6,7,8-tetrahydropteroylpenta-gamma-glutamateGallus sp.--485749 2D-image
10-formyl-5,8,10-trideazafolateGallus sp.--485753 2D-image
10-formylfolic acid pentaglutamateGallus sp.-competitive inhibitor485749 2D-image
2-[5-hydroxy-3-methyl-1-(2-methyl-4-sulfophenyl)-1H-pyrazo]-4-sulfobenzoic acidGallus gallus-Acid Yellow 54, 326203-A, competitive against 10-formyltetrahydrofolate, IC50: 0.012 mM, inhibitor partially blocks the cofactor binding site659419 2D-image
2-[[([4-[2-(2,4-diamino-7H-pyrrolo[2,3-d]pyrimidin-5-yl)ethyl]phenyl]carbonyl)amino]methyl]-3-methylidenebutanedioic acidHomo sapiens-weak inhibition of enzymes of folate metabolism relevant to rheumatoid arthritis therapy such as thymidylate synthase EC 2.1.1.45, two formyltransferases of purine biosynthesis EC 2.1.2.2 and EC 2.1.2.3, and 5,10-methylenetetrahydrofolate reductase EC 1.5.1.20. Potent inhibition of dihydrofolate reductase702155 2D-image
2-[[([4-[2-(2,4-diaminopyrido[3,2-d]pyrimidin-6-yl)ethyl]phenyl]carbonyl)amino]methyl]-3-methylidenebutanedioic acidHomo sapiens-weak inhibition of enzymes of folate metabolism relevant to rheumatoid arthritis therapy such as thymidylate synthase EC 2.1.1.45, two formyltransferases of purine biosynthesis EC 2.1.2.2 and EC 2.1.2.3, and 5,10-methylenetetrahydrofolate reductase EC 1.5.1.20. Potent inhibition of dihydrofolate reductase702155 2D-image
2-[[([4-[2-(2,4-diaminoquinazolin-6-yl)ethyl]-2-fluorophenyl]carbonyl)amino]methyl]-3-methylidenebutanedioic acidHomo sapiens-weak inhibition of enzymes of folate metabolism relevant to rheumatoid arthritis therapy such as thymidylate synthase EC 2.1.1.45, two formyltransferases of purine biosynthesis EC 2.1.2.2 and EC 2.1.2.3, and 5,10-methylenetetrahydrofolate reductase EC 1.5.1.20. Potent inhibition of dihydrofolate reductase702155 2D-image
2-[[([4-[2-(2-amino-4-methylquinazolin-6-yl)ethyl]phenyl]carbonyl)amino]methyl]-3-methylidenebutanedioic acidHomo sapiens-weak inhibition of enzymes of folate metabolism relevant to rheumatoid arthritis therapy such as thymidylate synthase EC 2.1.1.45, two formyltransferases of purine biosynthesis EC 2.1.2.2 and EC 2.1.2.3, and 5,10-methylenetetrahydrofolate reductase EC 1.5.1.20. Potent inhibition of dihydrofolate reductase702155-
2-[[([5-[2-(2,4-diaminoquinazolin-6-yl)ethyl]-2,5-dihydrothiophen-2-yl]carbonyl)amino]methyl]-3-methylidenebutanedioic acidHomo sapiens-weak inhibition of enzymes of folate metabolism relevant to rheumatoid arthritis therapy such as thymidylate synthase EC 2.1.1.45, two formyltransferases of purine biosynthesis EC 2.1.2.2 and EC 2.1.2.3, and 5,10-methylenetetrahydrofolate reductase EC 1.5.1.20. Potent inhibition of dihydrofolate reductase702155 2D-image
4-acetamidophenolGallus sp.--485750 2D-image
4-Amino-10-methylpteroyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamic acidGallus sp.-methotrexate polyglutamate, competitive inhibitor485749 2D-image
4-amino-10-methylpteroylglutamic acidGallus sp.-methotrexate, non-competitive inhibitor172161, 172163, 485749, 485758, 485760 2D-image
4-amino-10-methylpteroylglutamic acidHomo sapiens-methotrexate, non-competitive inhibitor172161, 172163, 485754, 485758, 485760 2D-image
4-amino-4-deoxy-5,8,10-trideazapteroyl-D,L-40-methyleneglutamic acidHomo sapiens-weak inhibition of enzymes of folate metabolism relevant to rheumatoid arthritis therapy such as thymidylate synthase EC 2.1.1.45, two formyltransferases of purine biosynthesis EC 2.1.2.2 and EC 2.1.2.3, and 5,10-methylenetetrahydrofolate reductase EC 1.5.1.20. Potent inhibition of dihydrofolate reductase702155 2D-image
4-N-allyl-5-aminoimidazole-4-carboxamide ribonucleotideHomo sapiens--485756 2D-image
4-N-methyl-5-aminoimidazole-4-carboxamide ribonucleotideHomo sapiens--485756 2D-image
5'-phosphoribosyl-5-formamido-4-imidazolecarboxamideHomo sapiens-product inhibitor485756 2D-image
5-amino-1-beta-D-ribofuranosylimidazole 5'-phosphateHomo sapiens--485756 2D-image
5-amino-1-beta-D-ribofuranosylimidazole-4-carbonitrile 5'-phosphateHomo sapiens--485756 2D-image
5-amino-1-beta-D-ribofuranosylimidazole-4-carboxamidoxime 5'-phosphateHomo sapiens--485756 2D-image
5-amino-1-beta-D-ribofuranosylimidazole-4-carboxylate 5'-phosphateHomo sapiens--485756 2D-image
5-amino-4-nitro-1-beta-D-ribofuranosylimidazole 5'-phosphateHomo sapiens--485756 2D-image
5-amino-4-nitroimidazole ribonucleotideMus musculus--485749 2D-image
5-formyl-5-aminoimidazole-4-carboxamide ribonucleotideMus musculus--485749 2D-image
5-formyl-5-aminoimidazole-4-carboxamide ribonucleotideHomo sapiens--485756 2D-image
7,8-dihydropteroylpenta-gamma-glutamateGallus sp.--485749 2D-image
Acid Yellow 17Gallus gallus-IC50: 0.012 mM659419 2D-image
AMPHomo sapiens--485756 2D-image
AzathioprineGallus sp.-competitive inhibitor485750 2D-image
AzathioprineMus musculus--485750 2D-image
beta-DADFGallus gallus-IC50 125 nM657946 2D-image
BW1540Homo sapiens-sulfamido-bridged 5,8-dideazafolate analogue652530 2D-image
BW2315Homo sapiens-sulfamido-bridged 5,8-dideazafolate analogue652530 2D-image
Cappsin 1Homo sapiens-non-competitive, inhibition of activity by preventing the formation of dimers658284-
Cappsin 2Homo sapiens-some derivatives of Cappepsin are also inhibitory, inhibition of activity by preventing the formation of dimers658284-
Cu2+Gallus sp.--485742 2D-image
dihydrofolic acid pentaglutamateGallus sp.--485749 2D-image
dihydrofolic acid pentaglutamateHomo sapiens--485754 2D-image
folic acid pentaglutamateGallus sp.-competitive inhibitor485749-
IbuprofenGallus sp., Mus musculus-anti-inflammatory drug485750 2D-image
KClHomo sapiens--172161 2D-image
methotrexateGallus sp.--485749 2D-image
methotrexateHomo sapiens-enzyme phosphorylation dampens the methotrexate-mediated transformylase activity inhibition696946 2D-image
N-(4-[3-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-propyl]benzoyl)-L-glutamic acidHomo sapiens-dual inhibition of glycinamide ribonucleotide formyltransferase and, likely, 5-amino-4-imidazole carboxamide ribonucleotide formyltransferase, resulting in potent growth inhibition of human tumor cells KB and IGROV1 that express folate receptors705032 2D-image
N-(4-[4-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-butyl]benzoyl)-L-glutamic acidHomo sapiens-dual inhibition of glycinamide ribonucleotide formyltransferase and, likely, 5-amino-4-imidazole carboxamide ribonucleotide formyltransferase, resulting in potent growth inhibition of human tumor cells KB and IGROV1 that express folate receptors705032 2D-image
N-(4-[5-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-pentyl]benzoyl)-L-glutamic acidHomo sapiens-dual inhibition of glycinamide ribonucleotide formyltransferase and, likely, 5-amino-4-imidazole carboxamide ribonucleotide formyltransferase, resulting in potent growth inhibition of human tumor cells KB and IGROV1 that express folate receptors705032 2D-image
N-(4-[6-(2-amino-4-oxo-3,4-dihydrothieno[2,3-d]pyrimidin-6-yl)-hexyl]benzoyl)-L-glutamic acidHomo sapiens-dual inhibition of glycinamide ribonucleotide formyltransferase and, likely, 5-amino-4-imidazole carboxamide ribonucleotide formyltransferase, resulting in potent growth inhibition of human tumor cells KB and IGROV1 that express folate receptors705032 2D-image
N-(4-[[(2,4-diaminopteridin-6-yl)methyl](methyl)amino]benzoyl)-gamma-glutamyl-gamma-glutamyl-gamma-glutamyl-gamma-glutamylglutamic acidHomo sapiens--702155 2D-image
N-(4-[[(2,4-diaminopteridin-6-yl)methyl](methyl)amino]benzoyl)-gamma-glutamyl-gamma-glutamylglutamic acidHomo sapiens--702155 2D-image
N-(4-[[(2,4-diaminopteridin-6-yl)methyl](methyl)amino]benzoyl)glutamic acidHomo sapiens--702155 2D-image
N-([5-[2-(2,4-diaminoquinazolin-6-yl)ethyl]-2,3-dihydrothiophen-2-yl]carbonyl)-4-methylideneglutamic acidHomo sapiens--702155 2D-image
N-[4-[2-(2,4-diamino-7H-pyrrolo[2,3-d]pyrimidin-5-yl)ethyl]benzoyl]-4-methylideneglutamic acidHomo sapiens--702155 2D-image
N-[4-[2-(2,4-diaminopyrido[3,2-d]pyrimidin-6-yl)ethyl]benzoyl]-4-methylideneglutamic acidHomo sapiens--702155 2D-image
N-[4-[2-(2,4-diaminoquinazolin-6-yl)ethyl]-2-fluorobenzoyl]-4-methylideneglutamic acidHomo sapiens--702155 2D-image
N-[4-[2-(2,4-diaminoquinazolin-6-yl)ethyl]benzoyl]-4-methylideneglutamic acidHomo sapiens--702155 2D-image
N-[4-[2-(2-amino-4-methylquinazolin-6-yl)ethyl]benzoyl]-4-methylideneglutamic acidHomo sapiens--702155 2D-image
N10-formyltetrahydropteroylglutamateEscherichia coli--485744 2D-image
NaproxenGallus sp., Mus musculus-anti-inflammatory drug485750 2D-image
NCI324571-CGallus gallus-IC50: 0.042 mM659419 2D-image
NCI324572-FGallus gallus-IC50: 0.012 mM659419 2D-image
NCI3262203-AGallus gallus-IC50: 0.012 mM659419 2D-image
NCI47729-MGallus gallus-IC50: 0.003 mM659419 2D-image
NSC126445Homo sapiens-IC50: 0.010mM659681 2D-image
NSC170645Homo sapiens-IC50: 0.036 mM659681 2D-image
NSC26699Homo sapiens-IC50: 0.017 mM659681 2D-image
NSC292213Homo sapiens-IC50: 0.009 mM659681 2D-image
NSC30171Homo sapiens-IC50: 0.0006 mM659681 2D-image
NSC321237Homo sapiens-IC50: 0.106 mM659681 2D-image
NSC324571Homo sapiens-IC50: 0.020 mM659681 2D-image
NSC324572Homo sapiens-IC50: 0.012 mM659681 2D-image
NSC324981Homo sapiens-IC50: 0.020 mM659681 2D-image
NSC326203Homo sapiens-IC50: 0.012 mM659681 2D-image
NSC326211Homo sapiens-IC50: 0.204 mM659681 2D-image
NSC37031Homo sapiens-IC50: 0.008 mM659681 2D-image
NSC37173Homo sapiens-IC50: 0.004 mM659681 2D-image
NSC41806Homo sapiens-IC50: 0.055 mM659681 2D-image
NSC45592Homo sapiens-IC50: 0.012 mM659681 2D-image
NSC47729Homo sapiens-IC50: 0.003 mM659681 2D-image
NSC58046Homo sapiens-IC50: 0.008 mM659681 2D-image
NSC7524Homo sapiens-IC50: 0.231 mM659681 2D-image
NSC88915Homo sapiens-IC50: 0.014 mM659681 2D-image
paracetamolGallus sp.--485750 2D-image
pteroylpenta-gamma-glutamateGallus sp.--485749 2D-image
SALOR2Gallus gallus-IC50: 0.0014 mM659419 2D-image
sulphalazineHomo sapiens--485750 2D-image
Thioinosinic acidGallus sp.-competitive inhibitor485750 2D-image
Thioinosinic acidMus musculus--485750 2D-image
tylenolGallus sp.--485750 2D-image
XMPHomo sapiens--485756 2D-image

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
additional informationHomo sapiens-enzyme phosphorylation by anaplastic lymphoma kinase enhances enzymatic activity and dampens the methotrexate-mediated transformylase activity inhibition696946-

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.0602-(6R,6S)-10-formyltetrahydrofolic acidHomo sapiensP31939-172163 2D-image
0.00051-10-formyldihydrofolic acid pentaglutamateGallus sp.--485749 2D-image
0.0043-10-formyltetrahydrofolateGallus sp.-mutant K266R485757 2D-image
0.0076-10-formyltetrahydrofolateGallus sp.-wild-type485757 2D-image
0.01-10-formyltetrahydrofolateHomo sapiens--485759 2D-image
0.0679-10-formyltetrahydrofolateGallus sp.--485748 2D-image
0.2-10-formyltetrahydrofolatePisum sativum--485745 2D-image
0.031-5'-phosphoribosyl-5-amino-imidazolecarboxamidePisum sativum--485745 2D-image
0.0009-5-amino-imidazole-4-thiocarboxamide ribonucleotideHomo sapiens-10-formyldihydrofolate as cofactor485756 2D-image
0.0005-5-aminoimidazole-4-carboxamide ribonucleotideGallus sp.-mutant K266R485757 2D-image
0.0015-5-aminoimidazole-4-carboxamide ribonucleotideHomo sapiens-10-formyldihydrofolate as cofactor485756 2D-image
0.0019-5-aminoimidazole-4-carboxamide ribonucleotideHomo sapiens-10-formyltetrahydrofolate as cofactor485756 2D-image
0.0019-5-aminoimidazole-4-carboxamide ribonucleotideGallus sp.-wild-type485757 2D-image
0.01-5-aminoimidazole-4-carboxamide ribonucleotideHomo sapiens--485756 2D-image
0.0168-5-aminoimidazole-4-carboxamide ribonucleotideHomo sapiensP31939-172163 2D-image
0.0168-5-aminoimidazole-4-carboxamide ribonucleotideHomo sapiens--485760 2D-image
0.00183-N10-formyltetrahydropteroylheptaglutamateGallus sp.-25 mM KCl485746 2D-image
0.00313-N10-formyltetrahydropteroylheptaglutamateGallus sp.-150 mM KCl485746 2D-image
0.00164-N10-formyltetrahydropteroylhexaglutamateGallus sp.-25 mM KCl485746 2D-image
0.00195-N10-formyltetrahydropteroylhexaglutamateGallus sp.-150 mM KCl485746 2D-image
0.00165-N10-formyltetrahydropteroylmonoglutamateGallus sp.-25 mM KCl485746 2D-image
0.353-N10-formyltetrahydropteroylmonoglutamateGallus sp.-150 mM KCl485746 2D-image
0.00206-N10-formyltetrahydropteroylpentaglutamateGallus sp.-150 mM KCl485746 2D-image
0.00256-N10-formyltetrahydropteroylpentaglutamateGallus sp.-25 mM KCl485746 2D-image
0.00099-N10-formyltetrahydropteroyltetraglutamateGallus sp.-25 mM KCl485746 2D-image
0.00107-N10-formyltetrahydropteroyltetraglutamateGallus sp.-150 mM KCl485746 2D-image
0.00595-N10-formyltetrahydropteroyltriglutamateGallus sp.-150 mM KCl485746 2D-image
0.674-N10-formyltetrahydropteroyltriglutamateGallus sp.-25 mM KCl485746 2D-image
0.185-tetrahydrofolateHomo sapiens--485759 2D-image
0.00023-tetrahydrofolic acidGallus sp.--485749 2D-image

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.00137-10-formylfolic acid pentaglutamateGallus sp.--485749 2D-image
0.007-2-[5-hydroxy-3-methyl-1-(2-methyl-4-sulfophenyl)-1H-pyrazo]-4-sulfobenzoic acidGallus gallus-pH 7.5659419 2D-image
0.006-5-amino-1-beta-D-ribofuranosylimidazole 5'-phosphateHomo sapiens--485756 2D-image
0.026-5-amino-1-beta-D-ribofuranosylimidazole-4-carbonitrile 5'-phosphateHomo sapiens--485756 2D-image
0.01-5-amino-1-beta-D-ribofuranosylimidazole-4-carboxylate 5'-phosphateHomo sapiens--485756 2D-image
0.0007-5-amino-4-nitro-1-beta-D-ribofuranosylimidazole 5'-phosphateHomo sapiens--485756 2D-image
4e-05-5-amino-4-nitroimidazole ribonucleotideMus musculus--485749 2D-image
0.0007-5-amino-4-nitroimidazole ribonucleotideHomo sapiens--485756 2D-image
7e-05-5-formyl-5-aminoimidazole-4-carboxamide ribonucleotideMus musculus--485749 2D-image
0.0004-5-formyl-5-aminoimidazole-4-carboxamide ribonucleotideHomo sapiens--485756 2D-image
0.027-AMPHomo sapiens--485756 2D-image
0.09-AzathioprineMus musculus--485750 2D-image
0.12-AzathioprineGallus sp.--485750 2D-image
8e-06-BW1540Homo sapiens--652530 2D-image
6e-06-BW2315Homo sapiens--652530 2D-image
0.003-Cappsin 1Homo sapiens--658284-
0.00014-dihydrofolic acid pentaglutamateGallus sp.--485749 2D-image
8e-05-folic acid pentaglutamateGallus sp.--485749-
0.075-methotrexateGallus sp.--485749 2D-image
0.00315-methotrexate polyglutamateGallus sp.--485749-
1.5e-05-NSC30171Homo sapiens--659681 2D-image
0.039-Thioinosinic acidGallus sp.--485750 2D-image
0.11-Thioinosinic acidMus musculus--485750 2D-image
0.0012-XMPHomo sapiens--485756 2D-image

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.012-2-[5-hydroxy-3-methyl-1-(2-methyl-4-sulfophenyl)-1H-pyrazo]-4-sulfobenzoic acidGallus gallus-Acid Yellow 54, 326203-A, competitive against 10-formyltetrahydrofolate, IC50: 0.012 mM, inhibitor partially blocks the cofactor binding site659419 2D-image
0.012-Acid Yellow 17Gallus gallus-IC50: 0.012 mM659419 2D-image
0.042-NCI324571-CGallus gallus-IC50: 0.042 mM659419 2D-image
0.012-NCI324572-FGallus gallus-IC50: 0.012 mM659419 2D-image
0.012-NCI3262203-AGallus gallus-IC50: 0.012 mM659419 2D-image
0.003-NCI47729-MGallus gallus-IC50: 0.003 mM659419 2D-image
0.01-NSC126445Homo sapiens-IC50: 0.010mM659681 2D-image
0.036-NSC170645Homo sapiens-IC50: 0.036 mM659681 2D-image
0.017-NSC26699Homo sapiens-IC50: 0.017 mM659681 2D-image
0.009-NSC292213Homo sapiens-IC50: 0.009 mM659681 2D-image
0.0006-NSC30171Homo sapiens-IC50: 0.0006 mM659681 2D-image
0.106-NSC321237Homo sapiens-IC50: 0.106 mM659681 2D-image
0.02-NSC324571Homo sapiens-IC50: 0.020 mM659681 2D-image
0.012-NSC324572Homo sapiens-IC50: 0.012 mM659681 2D-image
0.02-NSC324981Homo sapiens-IC50: 0.020 mM659681 2D-image
0.012-NSC326203Homo sapiens-IC50: 0.012 mM659681 2D-image
0.204-NSC326211Homo sapiens-IC50: 0.204 mM659681 2D-image
0.008-NSC37031Homo sapiens-IC50: 0.008 mM659681 2D-image
0.004-NSC37173Homo sapiens-IC50: 0.004 mM659681 2D-image
0.055-NSC41806Homo sapiens-IC50: 0.055 mM659681 2D-image
0.012-NSC45592Homo sapiens-IC50: 0.012 mM659681 2D-image
0.003-NSC47729Homo sapiens-IC50: 0.003 mM659681 2D-image
0.008-NSC58046Homo sapiens-IC50: 0.008 mM659681 2D-image
0.231-NSC7524Homo sapiens-IC50: 0.231 mM659681 2D-image
0.014-NSC88915Homo sapiens-IC50: 0.014 mM659681 2D-image
0.0014-SALOR2Gallus gallus-IC50: 0.0014 mM659419 2D-image
0.000125-beta-DADFGallus gallus-IC50 125 nM657946 2D-image
additional information-N-(4-[[(2,4-diaminopteridin-6-yl)methyl](methyl)amino]benzoyl)glutamic acidHomo sapiens-value above 0.05702155 2D-image
additional information-N-([5-[2-(2,4-diaminoquinazolin-6-yl)ethyl]-2,3-dihydrothiophen-2-yl]carbonyl)-4-methylideneglutamic acidHomo sapiens-value above 0.05702155 2D-image
additional information-N-[4-[2-(2,4-diamino-7H-pyrrolo[2,3-d]pyrimidin-5-yl)ethyl]benzoyl]-4-methylideneglutamic acidHomo sapiens-value above 0.05702155 2D-image
additional information-N-[4-[2-(2,4-diaminopyrido[3,2-d]pyrimidin-6-yl)ethyl]benzoyl]-4-methylideneglutamic acidHomo sapiens-value above 0.05702155 2D-image
additional information-N-[4-[2-(2,4-diaminoquinazolin-6-yl)ethyl]-2-fluorobenzoyl]-4-methylideneglutamic acidHomo sapiens-value above 0.05702155 2D-image
additional information-N-[4-[2-(2,4-diaminoquinazolin-6-yl)ethyl]benzoyl]-4-methylideneglutamic acidHomo sapiens-value above 0.05702155 2D-image
additional information-N-[4-[2-(2-amino-4-methylquinazolin-6-yl)ethyl]benzoyl]-4-methylideneglutamic acidHomo sapiens-value above 0.05702155 2D-image

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
0.00067-Homo sapiens--172161
0.0371-Gallus sp.--485746
0.856-Gallus sp.--485747
1.19-Homo sapiensP31939-172163
additional information-Pisum sativum-1 unit = removal of 0.0001 mM of AICA-ribotide/0.5 ml/20 min at 37°C485745

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
7.4-Pisum sativum--485745

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
610.5Gallus sp.--485757
6.58.5Pisum sativum--485745

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
37-Pisum sativum--485745

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
050Pisum sativum--485745

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
5.49-Thermotoga maritima-calculated isoelectric point689976

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
bloodHomo sapiens--673127Manually annotated by BRENDA team
bulbAllium cepa--485745Manually annotated by BRENDA team
CCRF-CEM cellHomo sapiens-leukemia cell, T lymphoblastoid cell line172161, 172163, 485754Manually annotated by BRENDA team
CCRF-CEM cellHomo sapiens--696946, 702155Manually annotated by BRENDA team
Ehrlich ascites carcinoma cellMus musculus--485747, 485749Manually annotated by BRENDA team
embryoPisum sativum--485745Manually annotated by BRENDA team
HeLa cellHomo sapiens-cervical carcinoma cell line485754Manually annotated by BRENDA team
Hep-G2 cellHomo sapiens-hepatocellular carcinoma cell line485754, 485758Manually annotated by BRENDA team
IGROV-1 cellHomo sapiens--705032Manually annotated by BRENDA team
JB6 cellHomo sapiens--696946Manually annotated by BRENDA team
JURKAT cellHomo sapiens--696946Manually annotated by BRENDA team
KB cellHomo sapiens--705032Manually annotated by BRENDA team
leafDaucus carota, Petroselinum crispum, Spinacia oleracea, Trifolium sp.--485745Manually annotated by BRENDA team
liverGallus sp.--172161, 485745, 485746, 485747, 485748, 485749, 485750Manually annotated by BRENDA team
liverHomo sapiens--172161, 485750, 485754Manually annotated by BRENDA team
liverColumba sp.--485745Manually annotated by BRENDA team
lymphocyteHomo sapiens--485750Manually annotated by BRENDA team
Mac-1 cellHomo sapiens--696946Manually annotated by BRENDA team
MCF-7 cellHomo sapiens-breast cancer cell172161, 172163Manually annotated by BRENDA team
peripheral blood mononuclear cellHomo sapiens, Mus musculus--485750Manually annotated by BRENDA team
petioleSpinacia oleracea, Trifolium sp.--485745Manually annotated by BRENDA team
placentaHomo sapiens--485754, 485758Manually annotated by BRENDA team
rootAllium cepa, Spinacia oleracea, Trifolium sp.--485745Manually annotated by BRENDA team
seedPisum sativum--485745Manually annotated by BRENDA team
seedlingPisum sativum--485745Manually annotated by BRENDA team
SUP-M2 cellHomo sapiens--696946Manually annotated by BRENDA team
Sup-M2-TS cellHomo sapiens--696946Manually annotated by BRENDA team

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
microsomePisum sativum---485745Manually annotated by BRENDA team
mitochondrionPisum sativum--5739485745Manually annotated by BRENDA team
nucleusPisum sativum--5634485745Manually annotated by BRENDA team

PDBSCOPCATHORGANISM
4ehi, downloadSCOP (4ehi)CATH (4ehi)Campylobacter jejuni subsp. jejuni serotype O:2 (strain NCTC 11168)
1g8m, downloadSCOP (1g8m)CATH (1g8m)Gallus gallus
1m9n, downloadSCOP (1m9n)CATH (1m9n)Gallus gallus
1oz0, downloadSCOP (1oz0)CATH (1oz0)Gallus gallus
1thz, downloadSCOP (1thz)CATH (1thz)Gallus gallus
2b1g, downloadSCOP (2b1g)CATH (2b1g)Gallus gallus
2b1i, downloadSCOP (2b1i)CATH (2b1i)Gallus gallus
2iu0, downloadSCOP (2iu0)CATH (2iu0)Gallus gallus
2iu3, downloadSCOP (2iu3)CATH (2iu3)Gallus gallus
1p4r, downloadSCOP (1p4r)CATH (1p4r)Homo sapiens
1pkx, downloadSCOP (1pkx)CATH (1pkx)Homo sapiens
1pl0, downloadSCOP (1pl0)CATH (1pl0)Homo sapiens
3zzm, downloadSCOP (3zzm)CATH (3zzm)Mycobacterium tuberculosis
4a1o, downloadSCOP (4a1o)CATH (4a1o)Mycobacterium tuberculosis

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
49700-Thermotoga maritima-monomer, residues 1-452689976
64430-Homo sapiensP31939cDNA sequencing172163
64450-Homo sapiensP31939electrospray mass spectroscopy172163
65000-Homo sapiens-recombinant enzyme, SDS-PAGE485754
65630-Homo sapiensP31939gel filtration172163

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
dimerGallus sp.--172161, 485758, 485760
dimerHomo sapiens-2 * 64400, deduced from nucleotide sequence of cDNA172161
dimerHomo sapiens--485758, 485760
dimerSaccharomyces cerevisiae--485758
dimerHomo sapiens-crystal structure analysis, monomer contains two functionally independent domains transformylase and cyclohydrolase activities652530
dimerGallus gallus-crystal structure analysis, monomer contains two functionally independent domains transformylase and cyclohydrolase activities657946
dimerHomo sapiens-monomer-dimer equilibrium in solution, dimeric form is required for transformylase activity658026
dimerHomo sapiens-transformylase activity is formed upon dimerization of the enzyme658284
dimerThermotoga maritima--689976
monomerHomo sapiensP319391 * 64425, calculated from amino acid sequence172163
monomerHomo sapiens--485758

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
phosphoproteinHomo sapiens-bifunctional 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase associates with nucleoplasmin-anaplastic lymphoma kinase, and its phosphorylation requires anaplastic lymphoma kinase activity. Phosphorylation enhances enzymatic activity and dampens the methotrexate-mediated transformylase activity inhibition696946

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
crystallized in complex with its inhibitor 2-[5-hydroxy-3-methyl-1-(2-methyl-4-sulfophenyl)-1H-pyrazo]-4-sulfobenzoic acidGallus gallus-659419
recombinant His-tag fusion protein crystallized by sitting drop vapor diffusion method in complex with its inhibitor beta-DADFGallus gallus-657946
ATIC complex crystallized in monoclinic space group P2(1), a = 56.48 A, b = 107.88 A, c = 103.86 A and beta = 91.2°Gallus sp.-485746, 485759, 485760
ATIC complex crystallized in monoclinic space group P2(1), a = 56.48 A, b = 107.88 A, c = 103.86 A and beta = 91.2°; crystallized in a binary complex with methotrexate, single crystal at 96 K to 2.3 A resolution, monoclinic crystals, space group P2(1), with unit-cell dimensions a = 65.17, B = 105.93, c= 103.47 A, beta = 108.27 degreesGallus sp.-485755
-Homo sapiens-485757, 485758
sitting drop vapor diffusion method, co-crystallized in the presence of substrate with BW1540 and BW2315Homo sapiens-652530
the three-dimensional structure of TM1249 is determined to 1.88 A resolution by molecular replacementThermotoga maritima-689976

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
No entries in this field

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
much less stable during storage in maleate buffer than in Tris or phosphate solutionsGallus sp.-485742

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
2°C, if the solution of purified enzyme are stored for 3 or 4 months, a requirement for a specific formyl donor can then be shownGallus sp.-485742

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-Escherichia coli-485744
-Gallus gallus-657946
recombinant protein using His-tagGallus gallus-659419
-Gallus sp.-485742, 485746, 485750, 485760
inosinicase activity copurifiesGallus sp.-485747
partiallyGallus sp.-485745, 485749
recombinant avian ATIC, purified from E. coli expression systemGallus sp.-485755
-Homo sapiens-172161, 172163, 485754, 485759, 702155
recombinant protein using His-tagHomo sapiens-659419
-Mus musculus-485747
-Pisum sativum-485745
using a nickel-chelating resin and a Resource Q columnThermotoga maritima-689976

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
cloned and expressedBacillus subtilis, Escherichia coli-172163
expressed in Escherichia coli as His-tag fusion proteinGallus gallus-657946
expressed in Escherichia coli BL21.DE3 as His-tag fusion proteinGallus gallus-659419
ATICcDNA cloned into a pET28a vector and transformed into Escherichia coli BL21.DE3 for overexpressionGallus sp.-485760
purH gene cloning and sequencing of cDNAGallus sp.-172163
cloned from a hepatoma cDNA library; cloned from a placenta cDNA library, overproduced in Escherichia coliHomo sapiens-485758
cloned from a placenta cDNA library, overproduced in Escherichia coliHomo sapiens-485754
expressed in Escherichia coli BL21.DE3 as His-tag fusion proteinHomo sapiens-659419
native and mutant proteinsHomo sapiens-658026
purH gene, cloning and sequencing of cDNA, expressed in Escherichia coliHomo sapiensP31939172163
cloned and expressedSalmonella enterica subsp. enterica serovar Typhimurium-172163
into the plasmid pMH4 for expression in the Escherichia coli strain GeneHogsThermotoga maritima-689976

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
T116SHomo sapiens-AICAR transformylase/inosine monophosphate cyclohydrolase 346C>G polymorphism673127
additional informationHomo sapiens-several site directed mutants created with mutations in the inosine monophosphate cyclohydrolase domain of the enzyme658026

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
medicineGallus gallus-potential target for antineoplastic chemotherapy659419
medicineGallus sp.--172161, 172163, 485750, 485757, 485758, 485759, 485760
medicineGallus sp.-attractive anticancer target485755
medicineHomo sapiens-target for development of inhibitors with potential use as chemotherapeutic agents172161, 172163, 485754, 485756, 485757, 485758, 485759
medicineHomo sapiens--485750
medicineHomo sapiens-potential target for development of antineoplastic drugs; target for development of inhibitors with potential use as chemotherapeutic agents485760
medicineHomo sapiens-potential target for antineoplastic chemotherapy658284, 659419
medicineHomo sapiens-potential target for antineoplastic drug design659681
medicineMus musculus-animal model of systemic autoimmune disease485750

DISEASETITLE OF PUBLICATIONLINK TO PUBMED
ArthritisAssociation of the 5-aminoimidazole-4-carboxamide ribonucleotide transformylase gene with response to methotrexate in juvenile idiopathic arthritis. PubMed
Arthritis, Juvenile RheumatoidAssociation of the 5-aminoimidazole-4-carboxamide ribonucleotide transformylase gene with response to methotrexate in juvenile idiopathic arthritis. PubMed
Arthritis, RheumatoidEffects of methotrexate on nucleotide pools in normal human T cells and the CEM T cell line. PubMed
Arthritis, RheumatoidEvidence for the hypothesis that 10-formyldihydrofolate is the in vivo substrate for aminoimidazolecarboxamide ribotide transformylase. PubMed
Arthritis, RheumatoidMetabolism-blocked antifolates as potential anti-rheumatoid arthritis agents: 4-amino-4-deoxy-5,8,10-trideazapteroyl-d,l-4'-methyleneglutamic acid (CH-1504) and its analogs. PubMed
Breast NeoplasmsTargeting tumour proliferation with a small-molecule inhibitor of AICAR transformylase homodimerization. PubMed
Leukemia5-Aminoimidazole-4-carboxamide ribotide transformylase-IMP cyclohydrolase from human CCRF-CEM leukemia cells: purification, pH dependence, and inhibitors. PubMed
LeukemiaDual effects of pyrazofurin and 3-deazauridine upon pyrimidine and purine biosynthesis in mouse L1210 leukemia. PubMed
NeoplasmsCrystal structure of avian aminoimidazole-4-carboxamide ribonucleotide transformylase in complex with a novel non-folate inhibitor identified by virtual ligand screening. PubMed
NeoplasmsTargeting tumour proliferation with a small-molecule inhibitor of AICAR transformylase homodimerization. PubMed
Precursor Cell Lymphoblastic Leukemia-LymphomaNovel pyrrolo[2,3-d]pyrimidine antifolate TNP-351: cytotoxic effect on methotrexate-resistant CCRF-CEM cells and inhibition of transformylases of de novo purine biosynthesis. PubMed
PsoriasisOutcomes of methotrexate therapy for psoriasis and relationship to genetic polymorphisms. PubMed

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISMLINK TO PUBMEDSOURCE
172161Szabados, E.; Hindmarsh, E.J.; Phillips, L.; Duggleby, R.G.; Christopherson, R.I.5-Aminoimidazole-4-carboxamide ribotide transformylase-IMP cyclohydrolase from human CCRF-CEM leukemia cells: purification, pH dependence and inhibitorsBiochemistry3314237-142451994Bacillus subtilis, Escherichia coli, Gallus sp., Homo sapiens PubMed
172163Rayl, E.A.; Moroson, B.A.; Beardsley, G.P.The human purH gene product, 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase. Cloning, sequencing, expression, purification, kinetic analysis, and domain mappingJ. Biol. Chem.2712225-22331996Bacillus subtilis, Escherichia coli, Gallus sp., Homo sapiens, Salmonella enterica subsp. enterica serovar Typhimurium PubMed
485742Hartman, S.C.; Buchanan, J.M.Biosynthesis of the purines. XXVI. The Identification of the formyl donors of the transformylation reactionsJ. Biol. Chem.2341812-18161959Gallus sp. PubMed
485744Patrick, T.W.; Crosbie, G.W.Specificity of 4-aminoimidazole-5-carboxamide ribotide transformylase of Escherichia coliBiochem. J.12431-321971Escherichia coli-
485745Iwai, K.; Fujisawa, Y.; Suzuki, N.The accumulation of 5'-phosphoribosyl-5amino-4-imidazolecarboxamide in folate-deficient pea seedlings and the enzymatic reaction in which the compound is involvedAgric. Biol. Chem.36398-4081972Allium cepa, Columba sp., Daucus carota, Escherichia coli, Gallus sp., Petroselinum crispum, Pisum sativum, Spinacia oleracea, Trifolium sp.-
485746Baggott, J.E.; Krumdieck, C.L.Folylpoly-gamma-glutamates as cosubstrates of 10-formyltetrahydrofolate:5'-phosphoribosyl-5-amino-4-imidazole-carboxamide formyltransferaseBiochemistry181037-10411979Gallus sp.-
485747Mueller, W.T.; Benkovic, S.J.On the purification and mechanism of action of 5-aminoimidazole-4-carboxamide-ribonucleotide transformylase from chicken liverBiochemistry20337-3441981Gallus sp., Mus musculus PubMed
485748Smith, G.K.; Mueller, T.; Benkovic, P.A.; Benkovic, S.J.On the cofactor specificity of glycinamide ribonucleotide and 5-aminoimidazole-4-carboxamide ribonucleotide transformylase from chicken liverBiochemistry201241-12451981Gallus sp. PubMed
485749Baggott, J.E.; Vaughn, W.H.; Hudson, B.B.Inhibition of 5-aminoimidazole-4-carboxamide ribotide transformylase, adenosine deaminase and 5'-adenylate deaminase by polyglutamates of methotrexate and oxidized folates and by 5-aminoimidazole-4-carboxamide riboside and ribotideBiochem. J.236193-2001986Gallus sp., Mus musculus PubMed
485750Ha, T.; Morgan, S.L.; Vaughn, W.H; Eto, I.; Baggott, J.E.Detection of inhibition of 5-aminoimidazole-4-carboxamide ribotide transformylase by thioinosinic acid and azathioprine by a new colorimetric assayBiochem. J.272339-3421990Gallus sp., Homo sapiens, Mus musculus PubMed
485753Boger, D.L.; Haynes, N.E.; Warren, M.S.; Gooljarsingh, L.T.; Ramcharan, J.; Kitos, P.A.; Benkovic, S.J.Functionalized analogues of 5,8,10-trideazafolate as potential inhibitors of GAR Tfase or AICAR TfaseBioorg. Med. Chem.51831-18381997Gallus sp. PubMed
485754Sugita, T.; Aya, H.; Ueno, M.; Ishizuka, T.; Kawashima, K.Characterization of molecularly cloned human 5-aminoimidazole-4-carboxamide ribonucleotide transformylaseJ. Biochem.122309-3131997Bacillus subtilis, Homo sapiens, Salmonella enterica subsp. enterica serovar Typhimurium PubMed
485755Reyes, V.M.; Greasley, S.E.; Stura, E.A.; Beardsley, G.P.; Wilson, I.A.Crystallization and preliminary crystallographic investigations of avian 5-aminoimidazole-4-carboxamide ribonucleotide transformylase-inosine monophosphate cyclohydrolase expressed in Escherichia coliActa Crystallogr. Sect. D561051-10542000Gallus sp.-
485756Wall, M.; Shim, J.H.; Benkovic, S.J.Human AICAR transformylase: Role of the 4-Carboxamide of AICAR in binding and catalysisBiochemistry3911303-113112000Escherichia coli, Homo sapiens PubMed
485757Shim, J.H.; Wall, M.; Benkovic, S.J.; Diaz, N.; Suarez, D.; Merz, K.M., Jr.Evaluation of the catalytic mechanism of AICAR transformylase by pH-dependent kinetics, mutagenesis, and quantum chemical calculationsJ. Am. Chem. Soc.1234687-46962001Bacillus subtilis, Escherichia coli, Gallus sp., Homo sapiens, Salmonella enterica subsp. enterica serovar Typhimurium PubMed
485758Vergis, J.M.; Bulock, K.G.; Fleming, K.G.; Beardsley, G.P.Human 5-aminoimidazole-4-carboxamide ribonucleotide transformylase/inosine 5'-monophosphate cyclohydrolase. A bifunctional protein requiring dimerization for transformylase activity but not for cyclohydrolase activityJ. Biol. Chem.2767727-77332001Gallus sp., Homo sapiens, Saccharomyces cerevisiae PubMed
485759Bulock, K.G.; Beardsley, G.P.; Anderson, K.S.The kinetic mechanism of the human bifunctional enzyme ATIC (5-amino-4-imidazolecarboxamide ribonucleotide transformylase/inosine 5'-monophosphate cyclohydrolase): A surprising lack of substrate channelingJ. Biol. Chem.27722168-221742002Gallus sp., Homo sapiens PubMed
485760Wolan, D.W.; Greasley, S.E.; Beardsley, G.P.; Wilson, I.A.Structural insights into the avian AICAR transformylase mechanismBiochemistry4115505-155132002Gallus sp., Homo sapiens PubMed
652530Cheong, C.G.; Wolan, D.W.; Greasley, S.E.; Horton, P.A.; Beardsley, G.P.; Wilson, I.A.Crystal structures of human bifunctional enzyme aminoimidazole-4-carboxamide ribonucleotide transformylase/IMP cyclohydrolase in complex with potent sulfonyl-containing antifolatesJ. Biol. Chem.27918034-180452004Homo sapiens PubMed
657946Wolan, D.W.; Greasley, S.E.; Wall, M.J.; Benkovic, S.J.; Wilson, I.A.Structure of avian AICAR transformylase with a multisubstrate adduct inhibitor beta-DADF identifies the folate binding siteBiochemistry4210904-109142003Gallus gallus PubMed
658026Vergis, J.M.; Beardsley, G.P.Catalytic mechanism of the cyclohydrolase activity of human aminoimidazole carboxamide ribonucleotide formyltransferase/inosine monophosphate cyclohydrolaseBiochemistry431184-11922004aves, Homo sapiens PubMed
658284Capps, K.J.; Humiston, J.; Dominique, R.; Hwang, I.; Boger, D.L.Discovery of AICAR Tfase inhibitors that disrupt requisite enzyme dimerizationBioorg. Med. Chem. Lett.152840-28442005Homo sapiens PubMed
659419Xu, L.; Li, C.; Olson, A.J.; Wilson, I.A.Crystal structure of avian aminoimidazole-4-carboxamide ribonucleotide transformylase in complex with a novel non-folate inhibitor identified by virtual ligand screeningJ. Biol. Chem.27950555-505652004Gallus gallus, Homo sapiens PubMed
659681Li, C.; Xu, L.; Wolan, D.W.; Wilson, I.A.; Olson, A.J.Virtual screening of human 5-aminoimidazole-4-carboxamide ribonucleotide transformylase against the NCI diversity set by use of AutoDock to identify novel nonfolate inhibitorsJ. Med. Chem.476681-66902004Homo sapiens PubMed
673127Gellekink, H.; Blom, H.J.; den Heijer, M.Associations of common polymorphisms in the thymidylate synthase, reduced folate carrier and 5-aminoimidazole-4-carboxamide ribonucleotide transformylase/inosine monophosphate cyclohydrolase genes with folate and homocysteine levels and venous thrombosisClin. Chem. Lab. Med.45471-4762007Homo sapiens PubMed
689976Axelrod, H.L.; McMullan, D.; Krishna, S.S.; Miller, M.D.; Elsliger, M.; Abdubek, P.; Ambing, E.; Astakhova, T.; Carlton, D.; Chiu, H.; Clayton, T.; Duan, L.; Feuerhelm, J.; Grzechnik, S.K.; Hale, J.; Han, G.W.; Haugen, J.; Jaroszewski, L.; Jin, K.K.; Klock, H.E.; Knuth, M.W.; Koesema, E.; Morse, A.T.; Nigoghossian, E.; Okach, L.; Oommachen, S.; Paulsen, J.; Quijano, K.; Reyes, R.; Rife, C.L.; van den Bedem, H.; Weekes, D.; White, A.; Wolf, G.; Xu, Q.; Hodgson, K.O.; Wooley, J.; Deacon. A.M.; Godzik, A.; Lesley, S.A.; Wilson, I.A.Crystal structure of AICAR transformylase IMP cyclohydrolase (TM1249) from Thermotoga maritima at 1.88 ANG. resolutionProteins Struct. Funct. Bioinform.711042-10492008Thermotoga maritima PubMed
696946Boccalatte, F.E.; Voena, C.; Riganti, C.; Bosia, A.; DAmico, L.; Riera, L.; Cheng, M.; Ruggeri, B.; Jensen, O.N.; Goss, V.L.; Lee, K.; Nardone, J.; Rush, J.; Polakiewicz, R.D.; Comb, M.J.; Chiarle, R.; Inghirami, G.The enzymatic activity of 5-aminoimidazole-4-carboxamide ribonucleotide formyltransferase/IMP cyclohydrolase is enhanced by NPM-ALK: new insights in ALK-mediated pathogenesis and the treatment of ALCLBlood1132776-27902009Homo sapiens PubMed
702155McGuire, J.J.; Haile, W.H.Metabolism-blocked antifolates as potential anti-rheumatoid arthritis agents: 4-amino-4-deoxy-5,8,10-trideazapteroyl-D,L-4-methyleneglutamic acid (CH-1504) and its analogsBiochem. Pharmacol.771161-11722009Homo sapiens PubMed
705032Deng, Y.; Zhou, X.; Kugel Desmoulin, S.; Wu, J.; Cherian, C.; Hou, Z.; Matherly, L.H.; Gangjee, A.Synthesis and biological activity of a novel series of 6-substituted thieno[2,3-d]pyrimidine antifolate inhibitors of purine biosynthesis with selectivity for high affinity folate receptors over the reduced folate carrier and proton-coupled folate transpoJ. Med. Chem.522940-29512009Homo sapiens PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 2.1.2.3)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)