Information on EC 2.1.1.238 - mycinamicin VI 2''-O-methyltransferase:
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EC NUMBERCOMMENTARY
2.1.1.238-

RECOMMENDED NAMEGeneOntology No.
mycinamicin VI 2''-O-methyltransferase-

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
S-adenosyl-L-methionine + mycinamicin VI = S-adenosyl-L-homocysteine + mycinamicin III
show the reaction diagram		----
S-adenosyl-L-methionine + mycinamicin VI = S-adenosyl-L-homocysteine + mycinamicin III
show the reaction diagram		-Micromonospora griseorubida-714732

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

PATHWAYKEGG LinkMetaCyc Link
No entries in this field

SYSTEMATIC NAMEIUBMB Comments
S-adenosyl-L-methionine:mycinamicin VI 2''-O-methyltransferaseThe enzyme is involved in the biosynthesis of mycinamicin macrolide antibiotics. Requires Mg2+ for optimal activity.

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
MycEMicromonospora griseorubida--714732, 715050, 715054

CAS REGISTRY NUMBERCOMMENTARY
No entries in this field

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Micromonospora griseorubida-714732, 715054--Manually annotated by BRENDA team
Micromonospora griseorubida-715050Q83WF2UniProtManually annotated by BRENDA team
Micromonospora griseorubida A11725-715050Q83WF2UniProtManually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
malfunctionMicromonospora griseorubida-construction of a MycE disrupion mutant. The disruption mutant mainly produces mycinamicin VI715054
physiological functionMicromonospora griseorubida-the enzyme is involved in the biosynthesis of mycinamicin macrolide antibiotics. Mycinamicins represent a family of macrolide antibiotics with more than twenty members produced by the rare actinomycete Micromonospora griseorubida714732

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
S-adenosyl-L-methionine + mycinamicin VIS-adenosyl-L-homocysteine + mycinamicin III
show the reaction diagram		Micromonospora griseorubida--715054--?
S-adenosyl-L-methionine + mycinamicin VIS-adenosyl-L-homocysteine + mycinamicin III
show the reaction diagram		Micromonospora griseorubida-the enzyme is involved in the biosynthesis of mycinamicin macrolide antibiotics714732, 715054--?
S-adenosyl-L-methionine + mycinamicin VIS-adenosyl-L-homocysteine + mycinamicin III
show the reaction diagram		Micromonospora griseorubida-methylation of the C2''-OH of 6-deoxyallose. MycE possesses high substrate specificity. MycE is incapable of double methylation to generate mycinamicin IV714732--?

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
S-adenosyl-L-methionine + mycinamicin VIS-adenosyl-L-homocysteine + mycinamicin III
show the reaction diagram		Micromonospora griseorubida-the enzyme is involved in the biosynthesis of mycinamicin macrolide antibiotics714732, 715054--

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
No entries in this field

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
Co2+Micromonospora griseorubida-activation is about 50% of the activation obtained by Mg2+714732
Fe2+Micromonospora griseorubida-activation is about 70% of the activation obtained by Mg2+714732
Mg2+Micromonospora griseorubida-optimal activity in presence of 10 mM MgCl2714732
Mn2+Micromonospora griseorubida-activation is about 70% of the activation obtained by Mg2+714732

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
EDTAMicromonospora griseorubida-significantly lowers the activity of MycE even in the presence of 10 mM Mg2+714732 2D-image

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.0264-mycinamicin VIMicromonospora griseorubida-pH 7.4, 30°C714732 2D-image

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.083-mycinamicin VIMicromonospora griseorubida-pH 7.4, 30°C714732 2D-image

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
3.14-mycinamicin VIMicromonospora griseorubida-pH 7.4, 30°C714732312934

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
9-Micromonospora griseorubida--714732

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
50-Micromonospora griseorubida--714732

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
No entries in this field

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
No entries in this field

PDBSCOPCATHORGANISM
No entries in this field

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
?Micromonospora griseorubida-44800, SDS-PAGE714732

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
No entries in this field

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
recombinant N-terminal His6-tagged protein, purified by one-step Ni-NTA agarose chromatographyMicromonospora griseorubida-714732

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
MycE is cloned into pET28b for overexpression in Escherichia coli BL21 (DE3)Micromonospora griseorubida-714732

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
medicineMicromonospora griseorubida-the antibacterial activities of some mycinamicin products against Staphylococcus aureus are higher than those of clinical macrolide antibiotics erythromycin and leucomycin714732

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISMLINK TO PUBMEDSOURCE
714732Li, S.; Anzai, Y.; Kinoshita, K.; Kato, F.; Sherman, D.H.Functional analysis of MycE and MycF, two O-methyltransferases involved in the biosynthesis of mycinamicin macrolide antibioticsChembiochem101297-13012009Micromonospora griseorubida PubMed
715050Anzai, Y.; Saito, N.; Tanaka, M.; Kinoshita, K.; Koyama, Y.; Kato, F.Organization of the biosynthetic gene cluster for the polyketide macrolide mycinamicin in Micromonospora griseorubidaFEMS Microbiol. Lett.218135-1412003Micromonospora griseorubida PubMed
715054Tsukada, S.; Anzai, Y.; Li, S.; Kinoshita, K.; Sherman, D.H.; Kato, F.Gene targeting for O-methyltransferase genes, mycE and mycF, on the chromosome of Micromonospora griseorubida producing mycinamicin with a disruption cassette containing the bacteriophage phi C31 attB attachment siteFEMS Microbiol. Lett.304148-1562010Micromonospora griseorubida PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 2.1.1.238)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)