Information on EC 2.1.1.187 -

Information on EC 2.1.1.187 - 23S rRNA (guanine745-N1)-methyltransferase:

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EC NUMBER	COMMENTARY
2.1.1.187	-

RECOMMENDED NAME	GeneOntology No.
23S rRNA (guanine745-N1)-methyltransferase	GO:0052911

REACTION	REACTION DIAGRAM	COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
S-adenosyl-L-methionine + guanine745 in 23S rRNA = S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		-	-	-	-
S-adenosyl-L-methionine + guanine745 in 23S rRNA = S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		-	Acinetobacter sp.	Q9AEP4	660492

REACTION TYPE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

PATHWAY	KEGG Link	MetaCyc Link
No entries in this field

SYSTEMATIC NAME	IUBMB Comments
S-adenosyl-L-methionine:23S rRNA (guanine745-N1)-methyltransferase	The enzyme specifically methylates guanine745 at N1 in 23S rRNA.

SYNONYMS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
23S rRNA m1G745 methyltransferase	Escherichia coli	-	-	704259
23S rRNA:m1G745 methyltransferase	Escherichia coli	-	-	441502
ribosomal RNA(m1G)-methylase	Escherichia coli	-	-	440039
RlmA	Escherichia coli	P36999	ambiguous	441501
RlmA(I)	Escherichia coli	-	-	660369
RlmAI	Acinetobacter sp.	Q9AEP4	-	441501
RlmAI	Pseudomonas stutzeri	A4VMZ0	-	441501
RlmAI methyltransferase	Acinetobacter sp.	Q9AEP4	-	660492
rRNA large subunit methyltransferase I	Acinetobacter sp.	Q9AEP4	-	441501
rRNA large subunit methyltransferase I	Pseudomonas stutzeri	A4VMZ0	-	441501
rRNA(m1G)methylase	Escherichia coli	-	-	440039
YebH	Escherichia coli	-	-	704259
RlmAI	Azotobacter vinelandii	C1DSW3	-	441501
RlmAI	Enterobacter aerogenes, Erwinia chrysanthemi	additional information	-	441501
RlmAI	Escherichia coli	P36999	-	441501
RlmAI	Proteus mirabilis, Pseudomonas fluorescens, Pseudomonas putida	additional information	-	441501
RlmAI	Pseudomonas syringae	Q48F48	-	441501
RlmAI	Shewanella putrefaciens	additional information	-	441501
rRNA large subunit methyltransferase I	Azotobacter vinelandii	C1DSW3	-	441501
rRNA large subunit methyltransferase I	Enterobacter aerogenes, Erwinia chrysanthemi	additional information	-	441501
rRNA large subunit methyltransferase I	Escherichia coli	P36999	-	441501
rRNA large subunit methyltransferase I	Proteus mirabilis, Pseudomonas fluorescens, Pseudomonas putida	additional information	-	441501
rRNA large subunit methyltransferase I	Pseudomonas syringae	Q48F48	-	441501
rRNA large subunit methyltransferase I	Shewanella putrefaciens	additional information	-	441501

CAS REGISTRY NUMBER	COMMENTARY
50812-25-4	-

ORGANISM	COMMENTARY	LITERATURE	SEQUENCE CODE	SEQUENCE DB	SOURCE
Acinetobacter sp.	-	660492	Q9AEP4	SwissProt
Acinetobacter sp.	comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII; strain ADP1	441501	Q9AEP4	SwissProt
Escherichia coli	-	440039, 441500, 441502, 660369, 704259	P36999	SwissProt
Pseudomonas stutzeri	comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII	441501	A4VMZ0	UniProt
Azotobacter vinelandii	comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII	441501	C1DSW3	SwissProt
Enterobacter aerogenes	comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII	441501	additional information	-
Erwinia chrysanthemi	comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII	441501	additional information	-
Escherichia coli	comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII	441501	P36999	SwissProt
Proteus mirabilis	comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII	441501	additional information	-
Pseudomonas fluorescens	comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII	441501	additional information	-
Pseudomonas putida	comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII	441501	additional information	-
Pseudomonas syringae	comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII	441501	Q48F48	UniProt
Shewanella putrefaciens	comparative analysis of the RlmAI/RlmAII methyltransferase sequences. Gram-negative sequences align with RlmAI and the Gram-positives sequences with RlmAII	441501	additional information	-

GENERAL INFORMATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
malfunction	Escherichia coli	-	lack of G745 methylation results in reduced rates of protein synthesis and growth. Addition of recombinant plasmid-encoded rrmA to an rrmA-defficient strain remedies these defects	441500
malfunction	Escherichia coli	-	mutant lacking N1-methylation of G745 exhibits increased resistance to viomycin in addition to severe defects of growth characteristics	441502
malfunction	Acinetobacter sp.	Q9AEP4	slow growth of the Acinetobacter rlmAI knockout. The growth defect of the Acinetobacter rlmAI knockout is lost during serial passaging of the strain on agar plates. None of the Acinetobacter or Escherichia coli cells tested has regained their ability to methylate G745, and all are thus pseudorevertants. The putative second-site mutations are possibly not rescuing the lack of G745 methylation, but are perhaps compensating for some other function of RlmAI. Cell growth is not dependent on G745 methylation, and the RlmAI methyltransferase therefore has another primary function	660492
malfunction	Escherichia coli	-	a mutant deficient in N1-methylguanine745 modification shows a 40% decreased growth rate in rich media, a drastic reduction in loosely coupled ribosomes, a 20% decreased polypeptide chain elongation rate, and increased resistance to the ribosome binding antibiotic viomycin	704259

SUBSTRATE	PRODUCT	REACTION DIAGRAM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY/ Substrate	LITERATURE/ Substrate	COMMENTARY/ Product	LITERATURE/ Product	Reversibility r=reversible ir=irreversible ?=not specified
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Escherichia coli	-	-	441500, 704259	-	-	?
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Acinetobacter sp.	Q9AEP4	Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate	441501	-	-	?
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Pseudomonas stutzeri	A4VMZ0	Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas stutzeri shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro	441501	-	-	?
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Pseudomonas syringae	Q48F48	Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas syringae shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro	441501	-	-	?
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Escherichia coli	-	the methylation at guanine745 is confined to Gram-negative bacteria	704259	-	-	?
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Escherichia coli	-	methylated guanines are located in hairpin 35, in domain II of prokaryotic 23S rRNA. RrmA possesses two regions that may be responsible for specific interactions with their target nucleic acid sequences: a putative Zn-finger domain in the N-terminus and the variable domain close to the C-terminus, which indicates that the enzyme exhibits the primary structural organization distinct from other nucleic acid MTases, despite sharing the common catalytic domain	441502	-	-	?
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Escherichia coli	-	methylation of the N1 position of nucleotide G745 in hairpin 35 of Escherichia coli 23S ribosomal RNA. Progressive truncation of the rRNA substrate shows that structures in stem-loops 33, 34 and 35 are required for methylation by RrmA. Multiple contacts between nucleotides in these stem-loops and RrmA are confirmed in footprinting experiments. No other RrmA contact is evident elsewhere in the rRNA. The RrmA contact sites on the rRNA are inaccessible in ribosomal particles and, consistent with this, 50S subunits or 70S ribosomes are not substrates for RrmA methylation. Methylate their target nucleotides only in the free RNA	441500	-	-	?
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Escherichia coli	P36999	Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate	441501	-	-	?
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Shewanella putrefaciens	additional information	Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate	441501	-	-	?
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Azotobacter vinelandii	C1DSW3	Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Azotobacter vinelandii shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro	441501	-	-	?
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Enterobacter aerogenes	additional information	Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Enterobacter aerogenes shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro	441501	-	-	?
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Erwinia chrysanthemi	additional information	Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Erwinia crysanthemi shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro	441501	-	-	?
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Proteus mirabilis	additional information	Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Proteus mirabilis shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro	441501	-	-	?
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Pseudomonas fluorescens	additional information	Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas fluorescens shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro	441501	-	-	?
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Pseudomonas putida	additional information	Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas putida shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro	441501	-	-	?

NATURAL SUBSTRATES	NATURAL PRODUCTS	REACTION DIAGRAM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY SUBSTRATE	LITERATURE (Substrate)	COMMENTARY PRODUCT	LITERATURE (Product)
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Escherichia coli	-	-	441500	-	-
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Acinetobacter sp.	Q9AEP4	Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate	441501	-	-
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Pseudomonas stutzeri	A4VMZ0	Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas stutzeri shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro	441501	-	-
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Pseudomonas syringae	Q48F48	Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas syringae shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro	441501	-	-
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Escherichia coli	-	the methylation at guanine745 is confined to Gram-negative bacteria	704259	-	-
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Escherichia coli	P36999	Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate	441501	-	-
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Shewanella putrefaciens	additional information	Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate	441501	-	-
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Azotobacter vinelandii	C1DSW3	Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Azotobacter vinelandii shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro	441501	-	-
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Enterobacter aerogenes	additional information	Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Enterobacter aerogenes shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro	441501	-	-
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Erwinia chrysanthemi	additional information	Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Erwinia crysanthemi shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro	441501	-	-
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Proteus mirabilis	additional information	Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Proteus mirabilis shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro	441501	-	-
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Pseudomonas fluorescens	additional information	Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas fluorescens shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro	441501	-	-
S-adenosyl-L-methionine + guanine745 in 23S rRNA	S-adenosyl-L-homocysteine + N1-methylguanine745 in 23S rRNA		Pseudomonas putida	additional information	Gram-negative 23 S rRNAs are methylated at G745. 23S rRNA of Gram-positives is methylated at G748. The position of an RNA methylation defines a sharp division between the Gram-negative and Gram-positive bacteria. Specificity of methylation is determined solely by the methyltransferase enzyme and is independent of the origin of the rRNA substrate. Pseudomonas putida shows intrinsic 23S rRNA methylation at G745. No methylation is determined with the recombinant protein in vivo or in vitro	441501	-	-

COFACTOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

METALS and IONS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

INHIBITORS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

ACTIVATING COMPOUND	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

KM VALUE [mM]	KM VALUE [mM] Maximum	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

TURNOVER NUMBER [1/s]	TURNOVER NUMBER MAXIMUM[1/s]	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

kcat/KM VALUE [1/mMs^-1]	kcat/KM VALUE [1/mMs^-1] Maximum	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

Ki VALUE [mM]	Ki VALUE [mM] Maximum	INHIBITOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

IC50 VALUE [mM]	IC50 VALUE [mM] Maximum	INHIBITOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg]	SPECIFIC ACTIVITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

pH OPTIMUM	pH MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
7.5	-	Acinetobacter sp.	Q9AEP4	assay at	441501
7.5	-	Pseudomonas stutzeri	A4VMZ0	assay at	441501
7.5	-	Azotobacter vinelandii	C1DSW3	assay at	441501
7.5	-	Enterobacter aerogenes, Erwinia chrysanthemi	additional information	assay at	441501
7.5	-	Escherichia coli	P36999	assay at	441501
7.5	-	Proteus mirabilis, Pseudomonas fluorescens, Pseudomonas putida	additional information	assay at	441501
7.5	-	Pseudomonas syringae	Q48F48	assay at	441501
7.5	-	Shewanella putrefaciens	additional information	assay at	441501

pH RANGE	pH RANGE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

TEMPERATURE OPTIMUM	TEMPERATURE OPTIMUM MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
37	-	Acinetobacter sp.	Q9AEP4	assay at	441501
37	-	Pseudomonas stutzeri	A4VMZ0	assay at	441501
37	-	Azotobacter vinelandii	C1DSW3	assay at	441501
37	-	Enterobacter aerogenes, Erwinia chrysanthemi	additional information	assay at	441501
37	-	Escherichia coli	P36999	assay at	441501
37	-	Proteus mirabilis, Pseudomonas fluorescens, Pseudomonas putida	additional information	assay at	441501
37	-	Pseudomonas syringae	Q48F48	assay at	441501
37	-	Shewanella putrefaciens	additional information	assay at	441501

TEMPERATURE RANGE	TEMPERATURE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

pI VALUE	pI VALUE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

SOURCE TISSUE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	SOURCE
No entries in this field

LOCALIZATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	GeneOntology No.	LITERATURE	SOURCE
No entries in this field

PDB	SCOP	CATH	ORGANISM
No entries in this field

MOLECULAR WEIGHT	MOLECULAR WEIGHT MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

SUBUNITS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
dimer	Escherichia coli	-	2 * 31500	660369

POSTTRANSLATIONAL MODIFICATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

Crystallization/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
hanging drop vapor diffusion techniques at 22�C, crystal structure of RlmAI at 2.8 A resolution. The crystal structure of RlmAI has a well defined and largely positively charged W-shaped RNA-binding cleft formed by asymmetric dimerization	Escherichia coli	-	660369

pH STABILITY	pH STABILITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

TEMPERATURE STABILITY	TEMPERATURE STABILITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

GENERAL STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

ORGANIC SOLVENT	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

OXIDATION STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

STORAGE STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

Purification/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
-	Acinetobacter sp.	-	441501, 660492
-	Escherichia coli	-	441500, 441501, 660369
partial	Escherichia coli	-	440039
-	Shewanella putrefaciens	additional information	441501

Cloned/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
-	Acinetobacter sp.	Q9AEP4	441501
expression in Escherichia coli	Acinetobacter sp.	Q9AEP4	660492
-	Escherichia coli	-	441500, 441501, 660369
-	Shewanella putrefaciens	additional information	441501

EXPRESSION	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

ENGINEERING	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

Renatured/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

APPLICATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

REF.	AUTHORS	TITLE	JOURNAL	VOL.	PAGES	YEAR	ORGANISM	LINK TO PUBMED	SOURCE
440039	Isaksson, L.A.	Partial purification of ribosomal RNA(m1G)- and rRNA(m2G)-methylases from Escherichia coli and demonstration of some proteins affecting their apparent activity	Biochim. Biophys. Acta	312	122-133	1973	Escherichia coli	PubMed
441500	Hansen, L.H.; Kirpekar, F.; Douthwaite, S.	Recognition of nucleotide G745 in 23 S ribosomal RNA by the RrmA methyltransferase	J. Mol. Biol.	310	1001-1010	2001	Escherichia coli	PubMed
441501	Liu, M.; Douthwaite, S.	Methylation at nucleotide G745 or G748 in 23S rRNA distinguishes gram-negative from gram-positive bacteria	Mol. Microbiol.	44	195-204	2002	Acinetobacter sp., Azotobacter vinelandii, Enterobacter aerogenes, Erwinia chrysanthemi, Escherichia coli, Proteus mirabilis, Pseudomonas fluorescens, Pseudomonas putida, Pseudomonas stutzeri, Pseudomonas syringae, Shewanella putrefaciens	PubMed
441502	Bujnicki, J.M.; Blumenthal, R.M.; Rychlewski, L.	Sequence analysis and structure prediction of 23S rRNA:m1G methyltransferases reveals a conserved core augmented with a putative Zn-binding domain in the N-terminus and family-specific elaborations in the C-terminus	J. Mol. Microbiol. Biotechnol.	4	93-99	2002	Escherichia coli	PubMed
660369	Das, K.; Acton, T.; Chiang, Y.; Shih, L.; Arnold, E.; Montelione, G.T.	Crystal structure of RlmAI: implications for understanding the 23S rRNA G745/G748-methylation at the macrolide antibiotic-binding site	Proc. Natl. Acad. Sci. USA	101	4041-4046	2004	Escherichia coli	PubMed
660492	Liu, M.; Novotny, G.W.; Douthwaite, S.	Methylation of 23S rRNA nucleotide G745 is a secondary function of the RlmAI methyltransferase	RNA	10	1713-1720	2004	Acinetobacter sp.	PubMed
704259	Gustafsson, C.; Persson, B.C.	Identification of the rrmA gene encoding the 23S rRNA m1G745 methyltransferase in Escherichia coli and characterization of an m1G745-deficient mutant	J. Bacteriol.	180	359-365	1998	Escherichia coli	PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 2.1.1.187)

NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM

IUBMB Enzyme Nomenclature

PROSITE Database of protein families and domains

SYSTERS

Protein Mutant Database

InterPro (database of protein families, domains and functional sites)

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