Information on EC 2.1.1.107 - uroporphyrinogen-III C-methyltransferase

Information on EC 2.1.1.107 - uroporphyrinogen-III C-methyltransferase:

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EC NUMBER	COMMENTARY
2.1.1.107	-

RECOMMENDED NAME	GeneOntology No.
uroporphyrinogen-III C-methyltransferase	GO:0004851

REACTION	REACTION DIAGRAM	COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
S-adenosyl-L-methionine + precorrin-1 = S-adenosyl-L-homocysteine + precorrin-2		-	-	-	-
S-adenosyl-L-methionine + uroporphyrinogen III = S-adenosyl-L-homocysteine + precorrin-1		-	-	-	-

REACTION TYPE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
methyl group transfer	-	-	-	-

PATHWAY	KEGG Link	MetaCyc Link
Biosynthesis of secondary metabolites	01110	-
Metabolic pathways	01100	-
Porphyrin and chlorophyll metabolism	00860	-

SYSTEMATIC NAME

IUBMB Comments

S-adenosyl-L-methionine:uroporphyrinogen-III C-methyltransferase

This enzyme catalyses two sequential methylation reactions, the first forming precorrin-1 and the second leading to the formation of precorrin-2. It is the first of three steps leading to the formation of siroheme from uroporphyrinogen III. The second step involves an NAD+-dependent dehydrogenation to form sirohydrochlorin from precorrin-2 (EC 1.3.1.76, precorrin-2 dehydrogenase) and the third step involves the chelation of Fe2+ to sirohydrochlorin to form siroheme (EC 4.99.1.4, sirohydrochlorin ferrochelatase). In Saccharomyces cerevisiae, the last two steps are carried out by a single bifunctional enzyme, Met8p. In some bacteria, steps 1-3 are catalysed by a single multifunctional protein called CysG, whereas in Bacillus megaterium, three separate enzymes carry out each of the steps, with SirA being responsible for the above reaction. Also involved in the biosynthesis of cobalamin.

SYNONYMS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
adenosylmethionine-uroporphyrinogen III methyltransferase	-	-	-	-
CobA/HemD	Lactobacillus reuteri	-	-	714579
HemD-CobA	Desulfovibrio vulgaris	-	-	696120
NirE	Pseudomonas aeruginosa	-	-	703663, 719948
S-adenosyl-L-methionine dependent uroporphyrinogen III methylase	-	-	-	-
S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase	Pseudomonas aeruginosa	P95417	-	719948
S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase	Zea mays	-	-	676956
SAM-dependent uroporphyrinogen III methyltransferase	Pseudomonas aeruginosa	-	-	703663
sirohaem synthase	-	-	-	-
SUMT	Pseudomonas denitrificans	-	-	662638
SUMT	Zea mays	-	-	676956
SUMT	Pseudomonas aeruginosa	-	-	703663
SUMT	Geobacillus stearothermophilus, Geobacillus stearothermophilus V	Q6TA16	-	706702
uroporphyrinogen III C-methyltransferase	-	-	-	-
uroporphyrinogen III methylase	-	-	-	-
uroporphyrinogen III methyltransferase	Zea mays	-	-	676956
uroporphyrinogen III methyltransferase	Pseudomonas aeruginosa	-	-	703663
uroporphyrinogen III methyltransferase/synthase	Desulfovibrio vulgaris	-	-	696120
uroporphyrinogen III synthase/methyltransferase	Lactobacillus reuteri	-	-	714579
uroporphyrinogen methyltransferase,	-	-	-	-

CAS REGISTRY NUMBER	COMMENTARY
73665-99-3	-

ORGANISM	COMMENTARY	LITERATURE	SEQUENCE CODE	SEQUENCE DB	SOURCE
Arabidopsis thaliana	genomic DNA, cDNA has accession number L47479	485090	Q96532	SwissProt
Desulfovibrio vulgaris	gene cobA/hemD	696120	-	-
Escherichia coli	-	485083, 485084, 485091, 485092	-	-
Geobacillus stearothermophilus	-	485088	-	-
Geobacillus stearothermophilus	gene cobA	706702	Q6TA16	UniProt
Geobacillus stearothermophilus V	gene cobA	706702	Q6TA16	UniProt
Lactobacillus reuteri	-	714579	-	-
Lactobacillus reuteri CRL 1098	-	714579	-	-
Propionibacterium freudenreichii	-	485087	Q51720	SwissProt
Pseudomonas aeruginosa	-	719948	P95417	UniProt
Pseudomonas aeruginosa	strain PAO1, gene nirE	703663	-	-
Pseudomonas denitrificans	-	485085, 485092, 662638	-	-
Pseudomonas denitrificans	-	485086	P21632	GenBank
Saccharomyces cerevisiae	-	485092	-	-
Salmonella enterica subsp. enterica serovar Typhimurium	-	485092	-	-
Thermus thermophilus	-	660612	-	-
Zea mays	-	485089, 676956	-	-

GENERAL INFORMATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
metabolism	Desulfovibrio vulgaris G200, Desulfovibrio vulgaris Groningen, Desulfovibrio vulgaris Hildenborough ATCC 29579, Desulfovibrio vulgaris Hildenborough, Desulfovibrio vulgaris Marburg, Desulfovibrio vulgaris Miyazaki F., Desulfovibrio vulgaris Miyazaki F, Desulfovibrio vulgaris Miyazaki, Desulfovibrio vulgaris	-	the enzyme is involved in the tetrapyrrole biosynthesis. Uroporphyrinogen III represents the first branch point in the pathway, where the action of enzymes such as HemE, F (N) and G results in the formation of protoporphyrin IX, a precursor of modified tetrapyrroles such as haem and chlorophyll. Alternatively, uroporphyrinogen III can undergo a S-adenosylmethionine-dependent transmethylation positions 2 and 7, by CysG, Met1p or CobA depending on the organism, to generate precorrin-2, a highly unstable yellow dipyrrocorphin, overview	696120
metabolism	Pseudomonas aeruginosa	-	the enzyme is important in the heme d1 biosynthesis, the essential prosthetic group of the dissimilatory nitrite reductase cytochrome cd1 requiring the methylation of the tetrapyrrole precursor uroporphyrinogen III at positions C-2 and C-7, pathway overview	703663
metabolism	Lactobacillus reuteri 121, Lactobacillus reuteri 21, Lactobacillus reuteri ATCC 55739, Lactobacillus reuteri ATCC PTA 6475, Lactobacillus reuteri BR11, Lactobacillus reuteri CRL 1098, Lactobacillus reuteri CRL1098, Lactobacillus reuteri CRL 1100, Lactobacillus reuteri CRL 1101, Lactobacillus reuteri L103, Lactobacillus reuteri L461, Lactobacillus reuteri MEP181R88, Lactobacillus reuteri RT-5, Lactobacillus reuteri TMW1.106, Lactobacillus reuteri	-	uroporphyrinogen III synthase/methyltransferase is a key bifunctional enzyme in the biosynthesis of cobalamin and other tetrapyrrols	714579
physiological function	Geobacillus stearothermophilus, Geobacillus stearothermophilus V	Q6TA16	the role of SUMT methyltransferase in tellurite(ate) detoxification is not related to tellurium volatilization	706702

SUBSTRATE	PRODUCT	REACTION DIAGRAM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY/ Substrate	LITERATURE/ Substrate	COMMENTARY/ Product	LITERATURE/ Product	Reversibility r=reversible ir=irreversible ?=not specified
S-adenosyl-L-methionine + heptacarboxyporphyrinogen III	?		Pseudomonas denitrificans	-	-	485085	-	-	?
S-adenosyl-L-methionine + hexacarboxyporphyrinogen III	?		Pseudomonas denitrificans	-	-	485085	-	-	?
S-adenosyl-L-methionine + pentacarboxyporphyrinogen III	?		Pseudomonas denitrificans	-	-	485085	-	-	?
S-adenosyl-L-methionine + uroporphyrinogen I	?		Pseudomonas denitrificans	-	-	485085	-	-	?
S-adenosyl-L-methionine + uroporphyrinogen I	?		Escherichia coli	-	methylates positions 2 and 7	485083	-	-	?
S-adenosyl-L-methionine + uroporphyrinogen I	?		Propionibacterium freudenreichii	Q51720	methylates positions 2 and 7	485087	-	-	?
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Pseudomonas denitrificans	-	-	485085	produces equal amounts of precorrin-1 and precorrin-2	485085	?
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Propionibacterium freudenreichii	Q51720	-	485087	after prolonged incubation (15 h) also tri- and tetra-methylated compounds formed, third methyl group at position 12	485087	?
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Salmonella enterica subsp. enterica serovar Typhimurium, Escherichia coli, Saccharomyces cerevisiae	-	-	485092	product is precorrin-2	485092	?
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Geobacillus stearothermophilus	-	-	485088	product is precorrin-2	485088	?
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Zea mays	-	-	485089	product is precorrin-2	485089	?
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Pseudomonas denitrificans	-	-	485085	product is precorrin-2	485085	?
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Pseudomonas denitrificans	-	-	485092	product is precorrin-2	485092	?
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Pseudomonas denitrificans	P21632	-	485086	product is precorrin-2	485086	?
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Propionibacterium freudenreichii	Q51720	-	485087	product is precorrin-2	485087	?
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Arabidopsis thaliana	Q96532	-	485090	product is precorrin-2	485090	?
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Escherichia coli	-	methylates positions 2 and 7	485083	product is precorrin-2	485083	?
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Escherichia coli	-	methylates positions 2 and 7	485084	product is precorrin-2	485084	?
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Escherichia coli	-	methylates positions 2 and 7	485091	product is precorrin-2	485091	?
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Escherichia coli	-	methylates positions 2 and 7	485092	product is precorrin-2	485092	?
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Escherichia coli	-	involved in biosynthesis of sirohydrochlorin	485083	product is precorrin-2	485083	?
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Escherichia coli	-	involved in biosynthesis of sirohydrochlorin	485084	product is precorrin-2	485084	?
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Propionibacterium freudenreichii	Q51720	involved in biosynthesis of sirohydrochlorin	485087	product is precorrin-2	485087	?
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Saccharomyces cerevisiae	-	involved in biosynthesis of siroheme	485092	product is precorrin-2	485092	?
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Zea mays	-	involved in biosynthesis of siroheme	485089	product is precorrin-2	485089	?
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Arabidopsis thaliana	Q96532	involved in biosynthesis of siroheme	485090	product is precorrin-2	485090	?
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Escherichia coli	-	involved in biosynthesis of cobalamin	485091	product is precorrin-2	485091	?
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Pseudomonas denitrificans	-	involved in biosynthesis of cobalamin	485085	product is precorrin-2	485085	?
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Pseudomonas denitrificans	P21632	involved in biosynthesis of cobalamin	485086	product is precorrin-2	485086	?
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Propionibacterium freudenreichii	Q51720	involved in biosynthesis of cobalamin	485087	product is precorrin-2	485087	?
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Arabidopsis thaliana	Q96532	no synthesis of cobalamin	485090	product is precorrin-2	485090	?
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Pseudomonas denitrificans SC510	-	involved in biosynthesis of cobalamin	485086	product is precorrin-2	485086	?
S-adenosyl-L-methionine + uroporphyrinogen III	?		Lactobacillus reuteri	-	-	714579	-	-	?
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + precorrin-2		Pseudomonas aeruginosa	P95417	-	719948	-	-	ir
uroporphyrinogen III + S-adenosyl-L-methionine	precorrin-2 + S-adenosyl-L-homocysteine		Pseudomonas aeruginosa	-	-	703663	-	-	?
uroporphyrinogen III + S-adenosyl-L-methionine	precorrin-2 + S-adenosyl-L-homocysteine		Desulfovibrio vulgaris	-	via precorrin-1	696120	-	-	?
uroporphyrinogen III + S-adenosyl-L-methionine	precorrin-2 + S-adenosyl-L-homocysteine		Pseudomonas aeruginosa	-	NirE binds S-adenosyl-L-methionine, although not with the same avidity as reported for other SAM-dependent uroporphyrinogen III methyltransferases involved in siroheme and cobalamin biosynthesis	703663	-	-	?
uroporphyrinogen III + S-adenosyl-L-methionine	precorrin-2 + S-adenosyl-L-homocysteine		Geobacillus stearothermophilus, Geobacillus stearothermophilus V	Q6TA16	the enzyme binds S-adenosyl-L-methionine and catalyzes the production of III methyl uroporphyrinogen in vitro	706702	-	-	?
additional information	?	-	Arabidopsis thaliana	Q96532	no formation of sirohydrochlorin in presence of NAD+ or NADP+	485090	-	-	-
additional information	?	-	Pseudomonas denitrificans	-	uroporphyrin III is not a substrate of the enzyme	485085	-	-	-
additional information	?	-	Desulfovibrio vulgaris	-	uroporphyrinogen III synthase is fused to uroporphyrinogen III methyltransferase in Desulfovibrio vulgaris	696120	-	-	-
additional information	?	-	Pseudomonas aeruginosa	-	at high enzyme concentrations NirE catalyzes an overmethylation of uroporphyrinogen III, resulting in the formation of trimethylpyrrocorphin	703663	-	-	-

NATURAL SUBSTRATES	NATURAL PRODUCTS	REACTION DIAGRAM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY SUBSTRATE	LITERATURE (Substrate)	COMMENTARY PRODUCT	LITERATURE (Product)
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Salmonella enterica subsp. enterica serovar Typhimurium, Escherichia coli	-	-	485092	product is precorrin-2	485092
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Geobacillus stearothermophilus	-	-	485088	product is precorrin-2	485088
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Pseudomonas denitrificans	-	-	485092	product is precorrin-2	485092
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Escherichia coli	-	involved in biosynthesis of sirohydrochlorin	485083	product is precorrin-2	485083
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Escherichia coli	-	involved in biosynthesis of sirohydrochlorin	485084	product is precorrin-2	485084
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Propionibacterium freudenreichii	Q51720	involved in biosynthesis of sirohydrochlorin	485087	product is precorrin-2	485087
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Saccharomyces cerevisiae	-	involved in biosynthesis of siroheme	485092	product is precorrin-2	485092
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Zea mays	-	involved in biosynthesis of siroheme	485089	product is precorrin-2	485089
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Arabidopsis thaliana	Q96532	involved in biosynthesis of siroheme	485090	product is precorrin-2	485090
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Escherichia coli	-	involved in biosynthesis of cobalamin	485091	product is precorrin-2	485091
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Pseudomonas denitrificans	-	involved in biosynthesis of cobalamin	485085	product is precorrin-2	485085
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Pseudomonas denitrificans	P21632	involved in biosynthesis of cobalamin	485086	product is precorrin-2	485086
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Propionibacterium freudenreichii	Q51720	involved in biosynthesis of cobalamin	485087	product is precorrin-2	485087
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Arabidopsis thaliana	Q96532	no synthesis of cobalamin	485090	product is precorrin-2	485090
S-adenosyl-L-methionine + uroporphyrinogen III	S-adenosyl-L-homocysteine + dihydrosirohydrochlorin		Pseudomonas denitrificans SC510	-	involved in biosynthesis of cobalamin	485086	product is precorrin-2	485086
uroporphyrinogen III + S-adenosyl-L-methionine	precorrin-2 + S-adenosyl-L-homocysteine		Pseudomonas aeruginosa	-	-	703663	-	-
uroporphyrinogen III + S-adenosyl-L-methionine	precorrin-2 + S-adenosyl-L-homocysteine		Desulfovibrio vulgaris	-	via precorrin-1	696120	-	-
additional information	?	-	Desulfovibrio vulgaris	-	uroporphyrinogen III synthase is fused to uroporphyrinogen III methyltransferase in Desulfovibrio vulgaris	696120	-	-

COFACTOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
S-adenosyl-L-methionine	Desulfovibrio vulgaris	-	-	696120	2D-image
S-adenosyl-L-methionine	Pseudomonas aeruginosa	-	dependent on	703663	2D-image
S-adenosyl-L-methionine	Geobacillus stearothermophilus, Geobacillus stearothermophilus V	Q6TA16	-	706702	2D-image

METALS and IONS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
Co2+	Pseudomonas denitrificans, Pseudomonas denitrificans CCUG 2519, Pseudomonas denitrificans G3575, Pseudomonas denitrificans SC510 Rif, Pseudomonas denitrificans SC510 Rifr(pXL1149), Pseudomonas denitrificans SC510 RifT, Pseudomonas denitrificans SC510 (RifT)	-	complete inhibition by 0.1 mM, mechanism unclear	485085

INHIBITORS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
S-adenosyl-L-homocysteine	Pseudomonas denitrificans	-	competitive	485085	2D-image
S-adenosyl-L-homocysteine	Pseudomonas aeruginosa	-	product inhibition at concentrations above 0.002 mM	703663	2D-image
S-adenosyl-L-homocysteine	Pseudomonas aeruginosa	P95417	-	719948	2D-image
uroporphyrinogen III	Pseudomonas denitrificans	-	substrate inhibition above 0.002 mM	485085	2D-image
uroporphyrinogen III	Pseudomonas aeruginosa	-	substrate inhibition at concentrations above 0.017 mM	703663	2D-image
additional information	Pseudomonas denitrificans	-	no inhibition by cobalamine or intermediates of synthesis	485085	-

ACTIVATING COMPOUND	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

KM VALUE [mM]	KM VALUE [mM] Maximum	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
0.0042	-	heptacarboxyporphyrinogen III	Pseudomonas denitrificans	-	-	485085	-
0.0047	-	hexacarboxyporphyrinogen III	Pseudomonas denitrificans	-	-	485085	-
0.0034	-	pentacarboxyporphyrinogen III	Pseudomonas denitrificans	-	-	485085	-
0.0063	-	S-adenosyl-L-methionine	Pseudomonas denitrificans	-	-	485085	2D-image
0.0004	-	uroporphyrinogen III	Desulfovibrio vulgaris	-	pH 8.0, 22�C, CobA/HemD	696120	2D-image
0.001	-	uroporphyrinogen III	Pseudomonas denitrificans	-	-	485085	2D-image

TURNOVER NUMBER [1/s]	TURNOVER NUMBER MAXIMUM[1/s]	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
38	-	uroporphyrinogen III	Pseudomonas denitrificans, Pseudomonas denitrificans CCUG 2519, Pseudomonas denitrificans G3575, Pseudomonas denitrificans SC510 Rif, Pseudomonas denitrificans SC510 Rifr(pXL1149), Pseudomonas denitrificans SC510 RifT, Pseudomonas denitrificans SC510 (RifT)	-	-	485085	2D-image

kcat/KM VALUE [1/mMs^-1]	kcat/KM VALUE [1/mMs^-1] Maximum	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

Ki VALUE [mM]	Ki VALUE [mM] Maximum	INHIBITOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
0.00032	-	S-adenosyl-L-homocysteine	Pseudomonas denitrificans, Pseudomonas denitrificans CCUG 2519, Pseudomonas denitrificans G3575, Pseudomonas denitrificans SC510 Rif, Pseudomonas denitrificans SC510 Rifr(pXL1149), Pseudomonas denitrificans SC510 RifT, Pseudomonas denitrificans SC510 (RifT)	-	-	485085	2D-image

IC50 VALUE [mM]	IC50 VALUE [mM] Maximum	INHIBITOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg]	SPECIFIC ACTIVITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
0.000044	-	Pseudomonas aeruginosa	-	recombinant enzyme	703663
0.003	-	Desulfovibrio vulgaris	-	purified recombinant CobA/HemD	696120
0.0062	-	Pseudomonas denitrificans	-	purified enzyme	485085
0.0091	-	Arabidopsis thaliana	Q96532	purified enzyme	485090

pH OPTIMUM	pH MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
7.7	7.8	Pseudomonas denitrificans	-	-	485085
8	-	Desulfovibrio vulgaris	-	assay at	696120
8	-	Pseudomonas aeruginosa	-	assay at	703663

pH RANGE	pH RANGE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

TEMPERATURE OPTIMUM	TEMPERATURE OPTIMUM MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
22	-	Desulfovibrio vulgaris, Desulfovibrio vulgaris G200, Desulfovibrio vulgaris Groningen, Desulfovibrio vulgaris Hildenborough, Desulfovibrio vulgaris Hildenborough ATCC 29579, Desulfovibrio vulgaris Marburg, Desulfovibrio vulgaris Miyazaki, Desulfovibrio vulgaris Miyazaki F., Desulfovibrio vulgaris Miyazaki F	-	assay at room temperature	696120
37	-	Pseudomonas aeruginosa	-	assay at	703663

TEMPERATURE RANGE	TEMPERATURE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

pI VALUE	pI VALUE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

SOURCE TISSUE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	SOURCE
No entries in this field

LOCALIZATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	GeneOntology No.	LITERATURE	SOURCE
chloroplast	Arabidopsis thaliana	Q96532	-	9507	485090
soluble	Propionibacterium freudenreichii	Q51720	-	-	485087
soluble	Thermus thermophilus	-	-	-	660612

PDB	SCOP	CATH	ORGANISM
2ybo, download	SCOP (2ybo)	CATH (2ybo)	Pseudomonas aeruginosa
2ybq, download	SCOP (2ybq)	CATH (2ybq)	Pseudomonas aeruginosa
1s4d, download	SCOP (1s4d)	CATH (1s4d)	Pseudomonas denitrificans
1pjq, download	SCOP (1pjq)	CATH (1pjq)	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
1pjs, download	SCOP (1pjs)	CATH (1pjs)	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
1pjt, download	SCOP (1pjt)	CATH (1pjt)	Salmonella typhimurium (strain LT2 / SGSC1412 / ATCC 700720)
1v9a, download	SCOP (1v9a)	CATH (1v9a)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
1ve2, download	SCOP (1ve2)	CATH (1ve2)	Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)

MOLECULAR WEIGHT	MOLECULAR WEIGHT MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
27000	-	Propionibacterium freudenreichii	Q51720	SDS-PAGE	485087
27610	-	Geobacillus stearothermophilus	-	calculated from DNA sequence	485088
28000	-	Geobacillus stearothermophilus	-	SDS-PAGE	485088
29200	-	Pseudomonas denitrificans	P21632	calculated from DNA sequence	485086
29200	-	Pseudomonas denitrificans SC510	-	calculated from DNA sequence	485086
30000	-	Pseudomonas denitrificans	-	SDS-PAGE	485085
39900	-	Arabidopsis thaliana	Q96532	calculated from DNA sequence	485090
41000	-	Arabidopsis thaliana	Q96532	SDS-PAGE	485090
49930	-	Escherichia coli	-	calculated from DNA-sequence	485084
50000	-	Geobacillus stearothermophilus	-	gel filtration	485088
52000	-	Escherichia coli	-	SDS-PAGE	485084
52000	-	Zea mays	-	gel filtration	676956
56000	-	Pseudomonas denitrificans	-	gel filtration	485085
60000	-	Pseudomonas aeruginosa	-	gel filtration	703663
60000	-	Geobacillus stearothermophilus, Geobacillus stearothermophilus V	Q6TA16	recombinant enzyme, native PAGE and gel filtration	706702
66000	-	Escherichia coli	-	gel filtration	485084
67000	-	Arabidopsis thaliana	Q96532	SDS-PAGE, uncleaved fusion protein	485090
79000	-	Zea mays	-	dynamic light scattering assay	676956

SUBUNITS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
?	Desulfovibrio vulgaris	-	x * 55000, about, recombinant CobA/HemD, SDS-PAGE	696120
?	Lactobacillus reuteri	-	x * 51000, SDS-PAGE	714579
dimer	Pseudomonas denitrificans	-	homodimer, 2 * 30000, SDS-PAGE, gel filtration	485085
dimer	Geobacillus stearothermophilus	-	homodimer, 2 * 27000, SDS-PAGE, gel filtration	485088
dimer	Thermus thermophilus	-	crystallization data, 2 * 26233, calculated	660612
dimer	Pseudomonas denitrificans	-	crystallization data	662638
homodimer	Zea mays	-	2 * 34000, SDS-PAGE	676956
homodimer	Pseudomonas aeruginosa	-	2 * 30000, SDS-PAGE	703663
homodimer	Geobacillus stearothermophilus, Geobacillus stearothermophilus V	Q6TA16	2 * 30000, recombinant enzyme, SDS-PAGE	706702
homodimer	Pseudomonas aeruginosa	P95417	-	719948
monomer	Escherichia coli	-	SDS-PAGE, gel filtration	485084

POSTTRANSLATIONAL MODIFICATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

Crystallization/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
sitting drop vapor diffusion method, using Tris, pH 8.0, 24% (w/v) PEG 6000 for enzyme without substrate, and 0.2 M lithium sulfate, 0.1 M Tris, pH 8.5, 1.26 M ammonium sulfate for enzyme in complex with uroporphyrinogen III	Pseudomonas aeruginosa	P95417	719948
enzyme topology diagramm	Pseudomonas denitrificans, Pseudomonas denitrificans CCUG 2519, Pseudomonas denitrificans G3575, Pseudomonas denitrificans SC510 (RifT), Pseudomonas denitrificans SC510 Rif, Pseudomonas denitrificans SC510 Rifr(pXL1149), Pseudomonas denitrificans SC510 RifT	-	662638
both apoform and with S-adenosyl-L-methionine	Thermus thermophilus	-	660612

pH STABILITY	pH STABILITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

TEMPERATURE STABILITY	TEMPERATURE STABILITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
65	-	Geobacillus stearothermophilus	-	thermostable, 17% of activity remaining after 10 min incubation	485088

GENERAL STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
complete loss of activity when treated with Triton X-100	Arabidopsis thaliana	Q96532	485090

ORGANIC SOLVENT	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

OXIDATION STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

STORAGE STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
4�C, 10 mM Tris, pH 7.7, stable for several weeks	Pseudomonas denitrificans, Pseudomonas denitrificans CCUG 2519, Pseudomonas denitrificans G3575, Pseudomonas denitrificans SC510 (RifT), Pseudomonas denitrificans SC510 Rif, Pseudomonas denitrificans SC510 Rifr(pXL1149), Pseudomonas denitrificans SC510 RifT	-	485085

Purification/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
from recombinant E. coli	Arabidopsis thaliana	Q96532	485090
recombinant CobA/HemD , HemD, and CobA from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Desulfovibrio vulgaris	-	696120
-	Escherichia coli	-	485084, 485091
from recombinant E. coli	Geobacillus stearothermophilus	-	485088
Ni2+-Sepharose column chromatography	Pseudomonas aeruginosa	P95417	719948
recombinant His-tagged NirE from Escherichia coli by metal chelate chromatography and gel filtration to homogeneity	Pseudomonas aeruginosa	-	703663
from recombinant E. coli using His-tag	Saccharomyces cerevisiae	-	485092
recombinant protein	Thermus thermophilus	-	660612
by Ni�NTA column	Zea mays	-	676956

Cloned/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
in Escherichia coli CBK103, amino acids 202-457 responsible for EC 2.1.1.107 activity	Arabidopsis thaliana	Q96532	485090
DNA and amino acid sequence determination and analysis, the uroporphyrinogen III synthase is naturally fused with the methyltransferase, bypassing the need for uroporphyrinogen III decarboxylase activity, overview. Expression of cobA/hemD and isolate hemD or cobA in Escherichia coli strain BL21(DE3)	Desulfovibrio vulgaris	-	696120
complementation experiments with cysteine autotroph strain	Escherichia coli	-	485091
cysG containing plasmid transformed into Escherichia coli CBK103; overexpression of cysG gene	Escherichia coli	-	485084
overexpression of cysG gene	Escherichia coli	-	485083
gene cobA, expression in Escherichia coli strain JM109(DE3), the recombinant enzyme renders the bacteria morre resistant to potassium tellurite and potassium tellurate	Geobacillus stearothermophilus	Q6TA16	706702
in Escherichia coli JM83 and MV1184	Geobacillus stearothermophilus	-	485088
gene cobA, expression in Escherichia coli strain JM109(DE3), the recombinant enzyme renders the bacteria morre resistant to potassium tellurite and potassium tellurate	Geobacillus stearothermophilus V	Q6TA16	706702
expressed in Escherichia coli BL21(DE3) cells	Lactobacillus reuteri, Lactobacillus reuteri 121, Lactobacillus reuteri 21, Lactobacillus reuteri ATCC 55739, Lactobacillus reuteri ATCC PTA 6475, Lactobacillus reuteri BR11, Lactobacillus reuteri CRL 1098, Lactobacillus reuteri CRL 1100, Lactobacillus reuteri CRL 1101, Lactobacillus reuteri CRL1098, Lactobacillus reuteri L103, Lactobacillus reuteri L461, Lactobacillus reuteri MEP181R88, Lactobacillus reuteri RT-5, Lactobacillus reuteri TMW1.106	-	714579
overexpression of cobA gene in Escherichia coli TB1	Propionibacterium freudenreichii	Q51720	485087
recombinant expression of C-terminally His-tagged NirE in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH10B	Pseudomonas aeruginosa	-	703663
50 fold increase of enzyme activity when overexpressed	Pseudomonas denitrificans	-	485085
expression of 5 open reading frames, codon 1120-1959 (cobA) encodes EC 2.1.1.107 activity	Pseudomonas denitrificans	P21632	485086
expression of 5 open reading frames, codon 1120-1959 (cobA) encodes EC 2.1.1.107 activity	Pseudomonas denitrificans SC510	-	485086
EC 2.1.1.107 deficient mutants complemented with cysG from Salmonella typhimurium and Escherichia coli and cobA from Pseudomonas denitrificans, overexpression of yeast gene product in Escherichia coli	Saccharomyces cerevisiae	-	485092
expression in Escherichia coli	Thermus thermophilus	-	660612
Escherichia coli JM109 used for cloning all recombinant plasmids and expressing the pUC18-derived plasmid, Escherichia coli strain S13009 and BL21(DE3) used for expressing the pQE-derived plasmids and the pET-derived plasmid	Zea mays	-	676956
in Escherichia coli CBK103	Zea mays	-	485089

EXPRESSION	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

ENGINEERING	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
D227A	Escherichia coli	-	full activity	485091
D248A	Escherichia coli	-	no transmethylase activity	485091
D303A	Escherichia coli	-	full activity	485091
G224A	Escherichia coli	-	unable to bind S-adenosyl-L-methionine	485091
K270I	Escherichia coli	-	full activity	485091
R298L	Escherichia coli	-	unable to bind S-adenosyl-L-methionine	485091
R309L	Escherichia coli	-	no transmethylase activity	485091
G12A	Geobacillus stearothermophilus, Geobacillus stearothermophilus V	Q6TA16	site-directed mutagenesis of cobA abolishes the tellurite resistance of the mesophilic, heterologous host Escherichia coli and the SUMT activity in vitro. Cells overexpressing SUMT G12A show 7fold less tolerance to K2TeO3 as compared to that exhibit by cells expressing the wild-type methyltransferase	706702
E114Q	Pseudomonas aeruginosa	P95417	the mutant shows strongly reduced activity compared to the wild type enzyme	719948
G189K	Pseudomonas aeruginosa	P95417	the mutant shows increased activity and reduced S-adenosyl-L-methionine-binding ability compared to the wild type enzyme	719948
G189N	Pseudomonas aeruginosa	P95417	the mutant shows increased activity and reduced S-adenosyl-L-methionine-binding ability compared to the wild type enzyme	719948
H161F	Pseudomonas aeruginosa	P95417	the mutant shows reduced activity compared to the wild type enzyme	719948
K102A	Pseudomonas aeruginosa	P95417	the mutant shows no activity and strongly reduced S-adenosyl-L-methionine-binding ability compared to the wild type enzyme	719948
R111K	Pseudomonas aeruginosa	P95417	the mutant shows strongly reduced activity and reduced S-adenosyl-L-methionine-binding ability compared to the wild type enzyme	719948
R149K	Pseudomonas aeruginosa	P95417	the mutant shows no activity compared to the wild type enzyme	719948
R51K	Pseudomonas aeruginosa	P95417	the mutant shows reduced activity and reduced S-adenosyl-L-methionine-binding ability compared to the wild type enzyme	719948
D47N	Pseudomonas denitrificans	-	binds about a quarter the quantity of S-adenosyl-L-methionine compared with wild-type, reaction product is only poxidised precorrin-1	662638
F106A	Pseudomonas denitrificans	-	considerably less soluble than wild-type, no binding of S-adenosyl-L-methionine, no enzymic activity	662638
L49A	Pseudomonas denitrificans	-	binds about half the quantity of S-adenosyl-L-methionine compared with wild-type, reaction products are oxidised forms of both precorrin-1 and precorrin-2	662638
M184A	Pseudomonas denitrificans	-	slight enzymic activity	662638
T130A	Pseudomonas denitrificans	-	considerably less soluble than wild-type, no binding of S-adenosyl-L-methionine	662638
Y183A	Pseudomonas denitrificans	-	considerably less soluble than wild-type, no binding of S-adenosyl-L-methionine, no enzyminc activity	662638
D47N	Pseudomonas denitrificans CCUG 2519	-	binds about a quarter the quantity of S-adenosyl-L-methionine compared with wild-type, reaction product is only poxidised precorrin-1	662638
F106A	Pseudomonas denitrificans CCUG 2519	-	considerably less soluble than wild-type, no binding of S-adenosyl-L-methionine, no enzymic activity	662638
L49A	Pseudomonas denitrificans CCUG 2519	-	binds about half the quantity of S-adenosyl-L-methionine compared with wild-type, reaction products are oxidised forms of both precorrin-1 and precorrin-2	662638
T130A	Pseudomonas denitrificans CCUG 2519	-	considerably less soluble than wild-type, no binding of S-adenosyl-L-methionine	662638
Y183A	Pseudomonas denitrificans CCUG 2519	-	considerably less soluble than wild-type, no binding of S-adenosyl-L-methionine, no enzyminc activity	662638
D47N	Pseudomonas denitrificans G3575	-	binds about a quarter the quantity of S-adenosyl-L-methionine compared with wild-type, reaction product is only poxidised precorrin-1	662638
F106A	Pseudomonas denitrificans G3575	-	considerably less soluble than wild-type, no binding of S-adenosyl-L-methionine, no enzymic activity	662638
L49A	Pseudomonas denitrificans G3575	-	binds about half the quantity of S-adenosyl-L-methionine compared with wild-type, reaction products are oxidised forms of both precorrin-1 and precorrin-2	662638
T130A	Pseudomonas denitrificans G3575	-	considerably less soluble than wild-type, no binding of S-adenosyl-L-methionine	662638
Y183A	Pseudomonas denitrificans G3575	-	considerably less soluble than wild-type, no binding of S-adenosyl-L-methionine, no enzyminc activity	662638
D47N	Pseudomonas denitrificans SC510 (RifT)	-	binds about a quarter the quantity of S-adenosyl-L-methionine compared with wild-type, reaction product is only poxidised precorrin-1	662638
F106A	Pseudomonas denitrificans SC510 (RifT)	-	considerably less soluble than wild-type, no binding of S-adenosyl-L-methionine, no enzymic activity	662638
L49A	Pseudomonas denitrificans SC510 (RifT)	-	binds about half the quantity of S-adenosyl-L-methionine compared with wild-type, reaction products are oxidised forms of both precorrin-1 and precorrin-2	662638
T130A	Pseudomonas denitrificans SC510 (RifT)	-	considerably less soluble than wild-type, no binding of S-adenosyl-L-methionine	662638
Y183A	Pseudomonas denitrificans SC510 (RifT)	-	considerably less soluble than wild-type, no binding of S-adenosyl-L-methionine, no enzyminc activity	662638
D47N	Pseudomonas denitrificans SC510 Rif	-	binds about a quarter the quantity of S-adenosyl-L-methionine compared with wild-type, reaction product is only poxidised precorrin-1	662638
F106A	Pseudomonas denitrificans SC510 Rif	-	considerably less soluble than wild-type, no binding of S-adenosyl-L-methionine, no enzymic activity	662638
L49A	Pseudomonas denitrificans SC510 Rif	-	binds about half the quantity of S-adenosyl-L-methionine compared with wild-type, reaction products are oxidised forms of both precorrin-1 and precorrin-2	662638
T130A	Pseudomonas denitrificans SC510 Rif	-	considerably less soluble than wild-type, no binding of S-adenosyl-L-methionine	662638
Y183A	Pseudomonas denitrificans SC510 Rif	-	considerably less soluble than wild-type, no binding of S-adenosyl-L-methionine, no enzyminc activity	662638
D47N	Pseudomonas denitrificans SC510 Rifr(pXL1149)	-	binds about a quarter the quantity of S-adenosyl-L-methionine compared with wild-type, reaction product is only poxidised precorrin-1	662638
F106A	Pseudomonas denitrificans SC510 Rifr(pXL1149)	-	considerably less soluble than wild-type, no binding of S-adenosyl-L-methionine, no enzymic activity	662638
L49A	Pseudomonas denitrificans SC510 Rifr(pXL1149)	-	binds about half the quantity of S-adenosyl-L-methionine compared with wild-type, reaction products are oxidised forms of both precorrin-1 and precorrin-2	662638
T130A	Pseudomonas denitrificans SC510 Rifr(pXL1149)	-	considerably less soluble than wild-type, no binding of S-adenosyl-L-methionine	662638
Y183A	Pseudomonas denitrificans SC510 Rifr(pXL1149)	-	considerably less soluble than wild-type, no binding of S-adenosyl-L-methionine, no enzyminc activity	662638
D47N	Pseudomonas denitrificans SC510 RifT	-	binds about a quarter the quantity of S-adenosyl-L-methionine compared with wild-type, reaction product is only poxidised precorrin-1	662638
F106A	Pseudomonas denitrificans SC510 RifT	-	considerably less soluble than wild-type, no binding of S-adenosyl-L-methionine, no enzymic activity	662638
L49A	Pseudomonas denitrificans SC510 RifT	-	binds about half the quantity of S-adenosyl-L-methionine compared with wild-type, reaction products are oxidised forms of both precorrin-1 and precorrin-2	662638
T130A	Pseudomonas denitrificans SC510 RifT	-	considerably less soluble than wild-type, no binding of S-adenosyl-L-methionine	662638
additional information	Desulfovibrio vulgaris	-	separation of the individual enzyme activities, uroporphyrinogen III synthase and uroporphyrinogen III methyltransferase, by dissecting the relevant gene to allow the production of two distinct proteins	696120
M186L	Pseudomonas aeruginosa	P95417	the mutant shows strongly reduced activity and reduced S-adenosyl-L-methionine-binding ability compared to the wild type enzyme	719948
additional information	Pseudomonas aeruginosa	-	construction of a nirE transposon mutant, which is not complemented by native cobA encoding the SAM-dependent uroporphyrinogen III methyltransferase involved in cobalamin formation, overview	703663
Y183A	Pseudomonas denitrificans SC510 RifT	-	considerably less soluble than wild-type, no binding of S-adenosyl-L-methionine, no enzyminc activity	662638
additional information	Zea mays	-	deletion mutants, truncated one deleting the C-terminal extra 52 amino acids actively expressed in Escherichia coli, the mature SUMT fusion mutant or the mature SUMT deleting the N-terminal 36 amino acids including glycine-rich region involved directly in SAM binding expressed as an inclusion body in Escherichia coli	676956

Renatured/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

APPLICATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
analysis	Zea mays	-	red fluorescent compounds are associated with the recombinant mature SUMT which are identified as sirohydrochlorin and trimethylpyrrocorphin by spectroscopic analysis, slightly alter the protein secondary structure	676956

REF.	AUTHORS	TITLE	JOURNAL	VOL.	PAGES	YEAR	ORGANISM (UNIPROT ACCESSION NO.)	LINK TO PUBMED	SOURCE
485083	Warren, M.J.; Gonzalez, M.D.; Williams, H.J.; Stolowich, N.J.; Scott, A.I.	Uroporpyrinogen III methylase catalyzes the enzymatic synthesis of sirohydrochlorines II and IV by a clockwise mechanism	J. Am. Chem. Soc.	112	5343-5345	1990	Escherichia coli	-
485084	Warren, M.J.; Roessner, C.A.; Santander, P.J.; Scott, A.I.	The Escherichia coli cysG gene encodes S-adenosylmethionine-dependent uroporphyrinogen III methylase	Biochem. J.	265	725-729	1990	Escherichia coli	PubMed
485085	Blanche, F.; Debussche, L.; Thibaut, D.; Crouzet, J.; Cameron, B.	Purification and characterization of S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase from Pseudomonas denitrificans	J. Bacteriol.	171	4222-4231	1989	Pseudomonas denitrificans	PubMed
485086	Crouzet, J.; Cauchois, L.; Blanche, F.; Debussche, L.; Thibaut, D.; Rouyez, M.C.; Rigault, S.; Mayaux, J.F.; Cameron, B.	Nucleotide sequence of a Pseudomonas denitrificans 5.4-kilobase DNA fragment containing five cob genes and identification of structural genes encoding S-adenosyl-L-methionine: uroporphyrinogen III methyltransferase and cobyrinic acid a,c-diamide synthase	J. Bacteriol.	172	5968-5979	1990	Pseudomonas denitrificans (P21632), Pseudomonas denitrificans	PubMed
485087	Sattler, I.; Roessner, C.A.; Stolowich, N.J.; Hardin, S.H.; Harris-Haller, L.W.; Yokubaitis, N.T.; Murooka, Y.; Hashimoto, Y.; Scott, A.I.	Cloning, sequencing, and expression of the uroporphyrinogen III methyltransferease cobA gene of Propionibacterium freudenreichii (shermanii)	J. Bacteriol.	177	1564-1569	1995	Propionibacterium freudenreichii, Propionibacterium freudenreichii (Q51720)	PubMed
485088	Chou, P.L.; Ohtsuka, M.; Minowa, T.; Yamasato, K.; Sakano, Y.; Matsuzawa, H.; Ohta, T.; Sakai, H.	Reddish Escherichia coli cells caused by overproduction of Bacillus stearothermophilus uroporphyrinogen III methylase: cloning, sequencing, and expression of the gene	Biosci. Biotechnol. Biochem.	59	1817-1824	1995	Geobacillus stearothermophilus	PubMed
485089	Sakakibara, H.; Takei, K.; Sugiyama, T.	Isolation and characterization of a cDNA that encodes maize uroporphyrinogen III methyltransferase, an enzyme involved in the synthesis of siroheme, which is a prosthetic group of nitrite reductase	Plant J.	10	883-892	1996	Zea mays	PubMed
485090	Leustek, T.; Smith, M.; Murillo, M.; Singh, D.P.; Smith, A.G.; Woodcock, S.C.; Awan, S.J.; Warren, M.J.	Siroheme biosynthesis in higher plants. Analysis of an S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase from Arabidopsis thaliana	J. Biol. Chem.	272	2744-2752	1997	Arabidopsis thaliana, Arabidopsis thaliana (Q96532)	PubMed
485091	Woodcock, S.C.; Raux, E.; Levillayer, F.; Thermes, C.; Rambach, A.; Warren, M.J.	Effect of mutations in the transmethylase and dehydrogenase/chelatase domains of siroheme synthase (CysG) on siroheme and cobalamin biosynthesis	Biochem. J.	330	121-129	1998	Escherichia coli	-
485092	Raux, E.; McVeigh, T.; Peters, S.E.; Leustek, T.; Warren, M.J.	The role of Saccharomyces cerevisiae MET1p and MET8p in sirohaem and cobalamin biosynthesis	Biochem. J.	338	701-708	1999	Escherichia coli, Pseudomonas denitrificans, Saccharomyces cerevisiae, Salmonella enterica subsp. enterica serovar Typhimurium	-
660612	Rehse, P.H.; Kitao, T.; Tahirov, T.H.	Structure of a closed-form uroporphyrinogen-III C-methyltransferase from Thermus thermophilus	Acta Crystallogr. Sect. D	61	913-919	2005	Thermus thermophilus	PubMed
662638	Vevodova, J.; Graham, R.M.; Raux, E.; Schubert, H.L.; Roper, D.I.; Brindley, A.A.; Ian Scott, A.; Roessner, C.A.; Stamford, N.P.; Elizabeth Stroupe, M.; Getzoff, E.D.; Warren, M.J.; Wilson, K.S.	Structure/function studies on a S-adenosyl-L-methionine-dependent uroporphyrinogen III C methyltransferase (SUMT), a key regulatory enzyme of tetrapyrrole biosynthesis	J. Mol. Biol.	344	419-433	2004	Pseudomonas denitrificans	PubMed
676956	Fan, J.; Wang, D.; Liang, Z.; Guo, M.; Teng, M.; Niu, L.	Maize uroporphyrinogen III methyltransferase: overexpression of the functional gene fragments in Escherichia coli and one-step purification	Protein Expr. Purif.	46	40-46	2006	Zea mays	PubMed
696120	Lobo, S.A.; Brindley, A.; Warren, M.J.; Saraiva, L.M.	Functional characterization of the early steps of tetrapyrrole biosynthesis and modification in Desulfovibrio vulgaris Hildenborough	Biochem. J.	420	317-325	2009	Desulfovibrio vulgaris, Desulfovibrio vulgaris (Q72DS3)	PubMed
703663	Storbeck, S.; Walther, J.; Mueller, J.; Parmar, V.; Schiebel, H.M.; Kemken, D.; Duelcks, T.; Warren, M.J.; Layer, G.	The Pseudomonas aeruginosa nirE gene encodes the S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferase required for heme d1 biosynthesis	FEBS J.	276	5973-5982	2009	Pseudomonas aeruginosa	PubMed
706702	Araya, M.A.; Tantalean, J.C.; Perez, J.M.; Fuentes, D.E.; Calderon, I.L.; Saavedra, C.P.; Burra, R.; Chasteen, T.G.; Vasquez, C.C.	Cloning, purification and characterization of Geobacillus stearothermophilus V uroporphyrinogen-III C-methyltransferase: evaluation of its role in resistance to potassium tellurite in Escherichia coli	Res. Microbiol.	160	125-133	2009	Geobacillus stearothermophilus, Geobacillus stearothermophilus (Q6TA16), Geobacillus stearothermophilus V (Q6TA16)	PubMed
714579	Vannini, V.; Rodriguez, A.; Vera, J.L.; de Valdez, G.F.; Taranto, M.P.; Sesma, F.	Cloning and heterologous expression of Lactobacillus reuteri uroporphyrinogen III synthase/methyltransferase gene (cobA/hemD): preliminary characterization	Biotechnol. Lett.	33	1625-1632	2011	Lactobacillus reuteri, Lactobacillus reuteri (Q50EH6), Lactobacillus reuteri CRL 1098, Lactobacillus reuteri CRL 1098 (Q50EH6)	PubMed
719948	Storbeck, S.; Saha, S.; Krausze, J.; Klink, B.U.; Heinz, D.W.; Layer, G.	Crystal structure of the heme d1 biosynthesis enzyme NirE in complex with its substrate reveals new insights into the catalytic mechanism of S-adenosyl-L-methionine-dependent uroporphyrinogen III methyltransferases	J. Biol. Chem.	286	26754-26767	2011	Pseudomonas aeruginosa, Pseudomonas aeruginosa (P95417)	PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 2.1.1.107)

NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM

IUBMB Enzyme Nomenclature

PROSITE Database of protein families and domains

SYSTERS

Protein Mutant Database

InterPro (database of protein families, domains and functional sites)

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