Information on EC 1.3.1.42 -

Information on EC 1.3.1.42 - 12-oxophytodienoate reductase:

Please wait a moment until all data are loaded. This message will disappear when all data are loaded.

Mark a special word or phrase in this record:
Select one or more organisms in this record:

The lowest common taxonomy group for this enzyme is: cellular organisms

Show additional data Do not include text mining results
Include

(text mining) results (more...)
Include

results (AMENDA + additional results, but less precise; more...)

Please login to have access to the AMENDA and FRENDA data

EC NUMBER	COMMENTARY
1.3.1.42	-

RECOMMENDED NAME	GeneOntology No.
12-oxophytodienoate reductase	GO:0016629

REACTION	REACTION DIAGRAM	COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
8-[(1R,2R)-3-oxo-2-{(Z)-pent-2-enyl}cyclopentyl]octanoate + NADP+ = (15Z)-12-oxophyto-10,15-dienoate + NADPH + H+		involved in the conversion of linolenate into jasmonate in Zea mays	-	-	-
8-[(1R,2R)-3-oxo-2-{(Z)-pent-2-enyl}cyclopentyl]octanoate + NADP+ = (15Z)-12-oxophyto-10,15-dienoate + NADPH + H+		-	Solanum lycopersicum	Q9FEW9, Q9FEX0, Q9XG54	656998
8-[(1R,2R)-3-oxo-2-{(Z)-pent-2-enyl}cyclopentyl]octanoate + NADP+ = (15Z)-12-oxophyto-10,15-dienoate + NADPH + H+		catalyzes the reduction of natural (+)-cis-12-oxophytodienoate for the jasmonic acid biosynthesis	Oryza sativa	-	689685

REACTION TYPE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
oxidation	-	-	-	-
redox reaction	-	-	-	-
redox reaction	Oryza sativa	-	-	689685
reduction	-	-	-	-
reduction	Oryza sativa	-	-	689685
reduction	Oryza sativa	Q8H9F1	;	690761
reduction	Solanum lycopersicum	-	;	699588
reduction	Arabidopsis thaliana	Q8GYB8, Q8LAH7, Q9FUP0	; ;	700835

PATHWAY	KEGG Link	MetaCyc Link
jasmonic acid biosynthesis	-	PWY-735

SYSTEMATIC NAME	IUBMB Comments
8-[(1R,2R)-3-oxo-2-{(Z)-pent-2-enyl}cyclopentyl]octanoate:NADP+ 4-oxidoreductase	Involved in the conversion of linolenate into jasmonate in Zea mays.

SYNONYMS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
12-oxo phytodienoic acid reductase	Zea mays	Q49HD7, Q49HD8, Q49HD9, Q49HE0, Q49HE1, Q49HE2, Q49HE3	-	689081
12-oxo-phytodienoate acid reductase	Arabidopsis thaliana	Q9FUP0	-	711596
12-oxo-phytodienoate acid reductase	Chlamydomonas reinhardtii, Medicago truncatula, Oryza sativa, Physcomitrella patens, Picea sitchensis, Populus trichocarpa, Selaginella moellendorffii, Sorghum bicolor, Volvox carteri, Zea mays	-	-	711596
12-oxo-phytodienoic acid reductase	-	-	-	-
12-oxo-phytodienoic acid reductase	Zea mays	-	-	689661
12-oxo-phytodienoic acid reductase	Oryza sativa	Q8H9F1	;	690761
12-oxophytodienoate reductase	Solanum lycopersicum	-	;	699588
12-oxophytodienoate reductase 3	Solanum lycopersicum	-	-	699588
12-oxophytodienoate-10,11-reductase	Corydalis sempervirens	-	-	390775, 390776, 390778
12-oxophytodienoate-10,11-reductase	Arabidopsis sp.	-	-	390776, 390778
12-oxophytodienoate10,11-reductase	Zea mays	Q49HD7, Q49HD8, Q49HD9, Q49HE0, Q49HE1, Q49HE2, Q49HE3	-	689081
morphine reductase	Pseudomonas putida	-	the enzyme is closely related to the isoforms of old yellow enzyme with major differences in the kinetic properties	678118
old yellow enzyme	Saccharomyces carlsbergensis, Saccharomyces cerevisiae	-	-	390775, 390778
OPDA reductase	Arabidopsis thaliana	Q8LAH7	-	390775
OPDA reductase	Oryza sativa	-	-	689685
OPDA reductase	Oryza sativa	Q8H9F1	;	690761
OPDA reductase I	Corydalis sempervirens	-	-	390776
OPR	Arabidopsis sp.	-	-	390778
OPR	Solanum lycopersicum	-	-	390780, 656998
OPR	Arabidopsis thaliana	-	-	656998, 657324, 711596
OPR	Oryza sativa	-	-	657134, 711596
OPR	Oryza sativa	Q8H9F1	;	690761
OPR	Arabidopsis thaliana	Q8GYB8, Q8LAH7, Q9FUP0	-	700835
OPR	Chlamydomonas reinhardtii, Medicago truncatula, Physcomitrella patens, Picea sitchensis, Populus trichocarpa, Selaginella moellendorffii, Sorghum bicolor, Volvox carteri, Zea mays	-	-	711596
OPR-1	Solanum lycopersicum	-	isozyme	684429
OPR-3	Solanum lycopersicum	-	isozyme	684429
OPR1	Arabidopsis thaliana	-	-	390776, 390777, 390778, 390779, 700835
OPR1	Corydalis sempervirens	-	-	390776, 390778, 390779
OPR1	Arabidopsis sp.	-	-	390776, 390778
OPR1	Solanum lycopersicum	-	-	390780, 699588
OPR1	Pisum sativum	Q76FR7, Q76FR8, Q76FR9, Q76FS0, Q76FS1, Q9AVK9	-	656802
OPR1	Zea mays	Q49HE4	isozyme	689081, 689661
OPR1	Oryza sativa	Q8H9F1	-	690761
OPR2	Arabidopsis thaliana	-	-	390777, 390778, 390779, 700835
OPR2	Arabidopsis sp.	-	-	390778
OPR2	Pisum sativum	Q76FR7, Q76FR8, Q76FR9, Q76FS0, Q76FS1, Q9AVK9	-	656802
OPR2	Zea mays	Q49HD7, Q49HD8, Q49HD9, Q49HE0, Q49HE1, Q49HE2, Q49HE3	isozyme	689081
OPR3	Arabidopsis thaliana	-	-	390779, 700835
OPR3	Pisum sativum	Q76FR7, Q76FR8, Q76FR9, Q76FS0, Q76FS1, Q9AVK9	-	656802
OPR3	Zea mays	Q49HD7, Q49HD8, Q49HD9, Q49HE0, Q49HE1, Q49HE2, Q49HE3	isozyme	689081
OPR3	Oryza sativa	Q8H9F1	-	690761
OPR3	Solanum lycopersicum	-	-	699588
OPR3 oxidoreductase	Arabidopsis sp.	-	-	390778
OPR4	Pisum sativum	Q76FR7, Q76FR8, Q76FR9, Q76FS0, Q76FS1, Q9AVK9	-	656802
OPR4	Zea mays	Q49HD7, Q49HD8, Q49HD9, Q49HE0, Q49HE1, Q49HE2, Q49HE3	isozyme	689081
OPR5	Pisum sativum	Q76FR7, Q76FR8, Q76FR9, Q76FS0, Q76FS1, Q9AVK9	-	656802
OPR5	Zea mays	Q49HD7, Q49HD8, Q49HD9, Q49HE0, Q49HE1, Q49HE2, Q49HE3	isozyme	689081
OPR6	Pisum sativum	Q76FR7, Q76FR8, Q76FR9, Q76FS0, Q76FS1, Q9AVK9	-	656802
OPR6	Zea mays	Q49HD7, Q49HD8, Q49HD9, Q49HE0, Q49HE1, Q49HE2, Q49HE3	isozyme	689081
OPR7	Zea mays	Q49HD7, Q49HD8, Q49HD9, Q49HE0, Q49HE1, Q49HE2, Q49HE3	isozyme	689081
OPR7	Oryza sativa	-	reduces both enantiomeric forms of 12-oxo-cis-phythodienoic acid	689685
OPR8	Zea mays	Q49HD7, Q49HD8, Q49HD9, Q49HE0, Q49HE1, Q49HE2, Q49HE3	isozyme	689081
OPRI	Corydalis sempervirens	-	-	390776
OPRII	Corydalis sempervirens	-	-	390776
oxophytodienoate reductase	Arabidopsis thaliana	Q8GYB8, Q8LAH7, Q9FUP0	-	700835
oxophytodienoic acid reductase	Pisum sativum	Q76FR7, Q76FR8, Q76FR9, Q76FS0, Q76FS1, Q9AVK9	-	656802
OYE	Saccharomyces cerevisiae	-	-	390775, 390776, 390778, 390779
phytodienoic acid reductase	Oryza sativa	Q8H9F1	;	690761
phytodienoic acid reductase	Solanum lycopersicum	-	;	699588
phytodienoic acid reductase	Arabidopsis thaliana	Q8GYB8, Q8LAH7, Q9FUP0	-	700835
S64	Pisum sativum	Q76FR7, Q76FR8, Q76FR9, Q76FS0, Q76FS1, Q9AVK9	i.e. isozyme PsOPR2	656802
S64	Pisum sativum	Q9AVK9	-	656976
cyclopentenone reductase	Zea mays	-	-	390772
additional information	Pisum sativum	Q76FR7, Q76FR8, Q76FR9, Q76FS0, Q76FS1, Q9AVK9	isozymes belong to subgroup I of the 12-oxophytodienoate reductase enzyme family	656802

CAS REGISTRY NUMBER	COMMENTARY
101150-03-2	-

ORGANISM	COMMENTARY	LITERATURE	SEQUENCE CODE	SEQUENCE DB	SOURCE
Arabidopsis sp.	-	390776, 390778	-	-
Arabidopsis thaliana	-	390775	Q8LAH7	UniProt
Arabidopsis thaliana	-	390776	-	-
Arabidopsis thaliana	-	711596	Q9FUP0	UniProt
Arabidopsis thaliana	(L.) Heynta wild-type ecotype Columbia(Col-0)	390777, 390778, 390779	-	-
Arabidopsis thaliana	ecotype Col-0, 3 isozymes OPR1-3	656998	-	-
Arabidopsis thaliana	ecotypes Col-0 and Wassilewskija	700835	Q8GYB8, Q8LAH7, Q9FUP0	UniProt
Arabidopsis thaliana	isoform OPR1, expression in Escherichia coli	677040	-	-
Arabidopsis thaliana	isozyme OPR3	657324	-	-
Avena sativa	oat, L. cv Moore	390773	-	-
Bryonia dioica	-	390777	-	-
Bryonia dioica	white bryony	390776	-	-
Chlamydomonas reinhardtii	-	711596	-	-
Corydalis sempervirens	-	390779	-	-
Corydalis sempervirens	rock harlequin	390775, 390776, 390778	-	-
Helianthus annuus	L. cv Sundak	390772, 390773	-	-
Linum usitatissimum	flax, L. cv Linott	390773	-	-
Medicago truncatula	-	711596	-	-
Oryza sativa	-	690761	Q8H9F1	SwissProt
Oryza sativa	-	690761, 711596	-	-
Oryza sativa	cultivar Nipponbare	689685	-	-
Oryza sativa	cv. BL-1, enzyme OsOPR1	657134	Q8H9F1	SwissProt
Physcomitrella patens	-	711596	-	-
Picea sitchensis	-	711596	-	-
Pisum sativum	cv. Midoriusui	656976	Q9AVK9	SwissProt
Pisum sativum	isozyme OPR1; 6 isozymes of enzyme family subgroup I	656802	Q76FS1	SwissProt
Pisum sativum	isozyme OPR2; 6 isozymes of enzyme family subgroup I	656802	Q9AVK9	SwissProt
Pisum sativum	isozyme OPR3; 6 isozymes of enzyme family subgroup I	656802	Q76FS0	SwissProt
Pisum sativum	isozyme OPR4; 6 isozymes of enzyme family subgroup I	656802	Q76FR9	SwissProt
Pisum sativum	isozyme OPR5; 6 isozymes of enzyme family subgroup I	656802	Q76FR8	SwissProt
Pisum sativum	isozyme OPR6; 6 isozymes of enzyme family subgroup I	656802	Q76FR7	SwissProt
Populus trichocarpa	-	711596	-	-
Pseudomonas putida	-	678118	-	-
Saccharomyces carlsbergensis	-	390775, 390778	-	-
Saccharomyces cerevisiae	yeast	390775, 390776, 390778, 390779	-	-
Selaginella moellendorffii	-	711596	-	-
Solanum lycopersicum	-	390780	-	-
Solanum lycopersicum	-	684429, 699588	Q9FEW9, Q9XG54	SwissProt
Solanum lycopersicum	isoform OPR3	676813	Q9FEW9	SwissProt
Solanum lycopersicum	isozyme OPR1; 3 isozymes OPR1-3	656998	Q9XG54	SwissProt
Solanum lycopersicum	isozyme OPR2, 12-oxophytodienoate-like protein; 3 isozymes OPR1-3, 12-oxophytodienoate-like protein	656998	Q9FEX0	SwissProt
Solanum lycopersicum	isozyme OPR3; 3 isozymes OPR1-3	656998	Q9FEW9	SwissProt
Solanum melongena	egg plant	390773	-	-
Sorghum bicolor	-	711596	-	-
Triticum aestivum	wheat, L. cv Thatcher	390773	-	-
Volvox carteri	-	711596	-	-
Zea mays	-	390774, 390776, 390778, 711596	-	-
Zea mays	corn, L. cv. NKPX443 Northrup king	390772, 390773	-	-
Zea mays	OPR1	689081, 689661	Q49HE4	UniProt
Zea mays	OPR2	689081	Q49HE3	UniProt
Zea mays	OPR3	689081	Q49HE2	UniProt
Zea mays	OPR4	689081	Q49HE1	UniProt
Zea mays	OPR5	689081	Q49HE0	UniProt
Zea mays	OPR6	689081	Q49HD9	UniProt
Zea mays	OPR7	689081	Q49HD8	UniProt
Zea mays	OPR8	689081	Q49HD7	UniProt

GENERAL INFORMATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
evolution	Arabidopsis thaliana	Q9FUP0	in plants, OPRs belong to the old yellow enzyme family and form multigene families, phylogenetic analysis, structural evolution and functional divergence among OPR paralogues in plants, 6 major green plant lineages, overview	711596
evolution	Chlamydomonas reinhardtii, Medicago truncatula, Oryza sativa, Physcomitrella patens, Picea sitchensis, Populus trichocarpa, Selaginella moellendorffii, Sorghum bicolor, Volvox carteri, Zea mays	-	in plants, OPRs belong to the old yellow enzyme family and form multigene families, phylogenetic analysis, structural evolution and functional divergence among OPR paralogues in plants, 6 major green plant lineages, overview	711596
physiological function	Arabidopsis thaliana	Q9FUP0	12-oxo-phytodienoic acid reductases are enzymes that catalyze the reduction of double-bonds in alpha,beta-unsaturated aldehydes or ketones and are part of the octadecanoid pathway that converts linolenic acid to jasmonic acid	711596
physiological function	Chlamydomonas reinhardtii, Medicago truncatula, Oryza sativa, Physcomitrella patens, Picea sitchensis, Populus trichocarpa, Selaginella moellendorffii, Sorghum bicolor, Volvox carteri, Zea mays	-	12-oxo-phytodienoic acid reductases are enzymes that catalyze the reduction of double-bonds in alpha,beta-unsaturated aldehydes or ketones and are part of the octadecanoid pathway that converts linolenic acid to jasmonic acid	711596

SUBSTRATE	PRODUCT	REACTION DIAGRAM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY/ Substrate	LITERATURE/ Substrate	COMMENTARY/ Product	LITERATURE/ Product	Reversibility r=reversible ir=irreversible ?=not specified
(15Z)-12-oxophyto-10,15-dienoate + NADPH	8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+		Zea mays	-	-	390773	-	-	-
(15Z)-12-oxophyto-10,15-dienoate + NADPH	8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+		Zea mays	-	-	390772	-	-	?
(15Z)-12-oxophyto-10,15-dienoate + NADPH	8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+		Arabidopsis thaliana	-	-	656998	-	-	?
(15Z)-12-oxophyto-10,15-dienoate + NADPH	8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+		Solanum melongena	-	-	390773	-	-	-
(15Z)-12-oxophyto-10,15-dienoate + NADPH	8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+		Solanum lycopersicum	Q9FEW9, Q9FEX0, Q9XG54	-	656998	-	-	?
(15Z)-12-oxophyto-10,15-dienoate + NADPH	8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+		Pisum sativum	Q9AVK9	-	656976	-	-	?
(15Z)-12-oxophyto-10,15-dienoate + NADPH	8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+		Oryza sativa	Q8H9F1	leads to jasmonate biosynthesis, preference of (-)-cis isomer over the (+)-cis-isomer	657134	-	-	?
(15Z)-12-oxophyto-10,15-dienoate + NADPH	8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+		Arabidopsis thaliana	-	racemic mixture as substrate is accepted by isozyme OPR1	656998	-	-	?
(2E)-3,7-dimethylocta-2,6-dienal + NADH	(3S)-3,7-dimethyloct-6-enal + NAD+		Solanum lycopersicum	Q9FEW9, Q9XG54	90% conversion with OPR-3	684429	-	-	?
(2E)-3,7-dimethylocta-2,6-dienal + NADH	(3S)-3,7-dimethyloct-6-enal + NAD+		Solanum lycopersicum	Q9FEW9, Q9XG54	above 99% conversion with OPR-1	684429	-	-	?
(2E)-3,7-dimethylocta-2,6-dienal + NADPH	(3S)-3,7-dimethyloct-6-enal + NADP+		Solanum lycopersicum	Q9FEW9, Q9XG54	90% conversion with OPR-3	684429	-	-	?
(2E)-3,7-dimethylocta-2,6-dienal + NADPH	(3S)-3,7-dimethyloct-6-enal + NADP+		Solanum lycopersicum	Q9FEW9, Q9XG54	above 99% conversion with OPR-1	684429	-	-	?
(2Z)-2-methylbut-2-enedioic acid + NADH	(2R)-2-methylbutanedioic acid + NAD+		Solanum lycopersicum	Q9FEW9, Q9XG54	above 99% conversion with OPR-1	684429	-	-	?
(2Z)-2-methylbut-2-enedioic acid + NADPH	(2R)-2-methylbutanedioic acid + NADP+		Solanum lycopersicum	Q9FEW9, Q9XG54	above 99% conversion with OPR-1	684429	-	-	?
(9R,13R)-(15Z)-12-oxophyto-10,15-dienoate + NADPH	(9R,13R)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+		Solanum lycopersicum	Q9FEW9, Q9FEX0, Q9XG54	-	656998	-	-	?
(9R,13R)-(15Z)-12-oxophyto-10,15-dienoate + NADPH	(9R,13R)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+		Solanum lycopersicum	Q9FEW9, Q9FEX0, Q9XG54	isozyme OPR1, no activity with the (9S,13S)-(15Z)-12-oxophyto-10,15-dienoate enantiomer	656998	-	-	?
(9R,13R)-(15Z)-12-oxophyto-10,15-dienoate + NADPH	(9R,13R)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+		Solanum lycopersicum	Q9FEW9, Q9FEX0, Q9XG54	isozymes OPR3	656998	-	-	?
(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH	(9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+		Arabidopsis thaliana	-	product is an intermediate in the jasmonate biosynthesis	657324	-	-	?
(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH	(9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+		Solanum lycopersicum	Q9FEW9, Q9FEX0, Q9XG54	product is the direct precursor in the jasmonate biosynthesis	656998	-	-	?
(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH	(9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+		Solanum lycopersicum	Q9FEW9, Q9FEX0, Q9XG54	isozyme OPR3	656998	-	-	?
(9S,13S)-12-oxo-phytodienoic acid + NADPH	3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+		Arabidopsis thaliana	-	-	390776	-	-	?
(9S,13S)-12-oxo-phytodienoic acid + NADPH	3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+		Solanum lycopersicum	Q9FEW9	-	676813	-	-	?
(9S,13S)-12-oxo-phytodienoic acid + NADPH	3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+		Arabidopsis thaliana	-	OPR3 is the isoenzyme relevant for jasmonate biosynthesis	390779	-	-	?
(9S,13S)-12-oxo-phytodienoic acid + NADPH	3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+		Corydalis sempervirens	-	OPRII reduces all 4 OPDA isomers	390776	-	-	?
(9S,13S)-12-oxo-phytodienoic acid + NADPH	3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+		Corydalis sempervirens	-	reductase specific for the natural cis-OPDA enatiomer encoded by OPR2	390779	-	-	?
(9S,13S)-12-oxo-phytodienoic acid + NADPH + H+	3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+		Solanum lycopersicum	-	enzyme is a FMN-dependent oxidoreductase that catalyzes the reduction of the cyclopentenone (9S,13S)-12-oxophytodienoate to the corresponding cyclopentanone in the biosynthesis of the plant hormone jasmonic acid	699588	-	-	?
(9S,13S)-12-oxo-phytodienoic acid + NADPH + H+	?		Solanum lycopersicum	-	FMN-dependent oxidoreductase, biosynthesis of the plant hormone jasmonic acid, the enzyme in plants relates to old yellow enzyme (OYE) from Saccharomyces cerevisiae	699588	-	-	?
(9S,13S)-12-oxophytodienoate + NADPH	3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+		Solanum lycopersicum	Q9FEW9, Q9XG54	-	684429	-	-	?
(9S,13S)-12-oxophytodienoic acid + NADPH + H+	3-2(2'(Z)-pentyl)cyclopentane-1-octanoic acid + NADP+		Arabidopsis thaliana	Q8GYB8, Q8LAH7, Q9FUP0	OPR3 is involved in jasmonic acid biosynthesis	700835	-	-	?
12-oxo-cis-phythodienoic acid + NADPH	cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+		Zea mays	-	-	390778	-	390778	?
12-oxo-cis-phythodienoic acid + NADPH	cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+		Oryza sativa	-	-	689685	-	-	?
12-oxo-cis-phythodienoic acid + NADPH	cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+		Solanum lycopersicum	Q9FEW9, Q9XG54	-	684429	-	-	?
12-oxo-cis-phythodienoic acid + NADPH	cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+		Zea mays	Q49HD7, Q49HD8, Q49HD9, Q49HE0, Q49HE1, Q49HE2, Q49HE3, Q49HE4	-	689081	-	-	?
12-oxo-cis-phythodienoic acid + NADPH	cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+		Zea mays	-	-	689661	-	-	?
12-oxo-cis-phythodienoic acid + NADPH	cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+		Triticum aestivum	-	third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA	390773	-	390773	?
12-oxo-cis-phythodienoic acid + NADPH	cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+		Zea mays	-	third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA	390772	-	-	?
12-oxo-cis-phythodienoic acid + NADPH	cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+		Zea mays	-	third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA	390773	-	390773	?
12-oxo-cis-phythodienoic acid + NADPH	cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+		Zea mays	-	third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA	390774	-	390774	?
12-oxo-cis-phythodienoic acid + NADPH	cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+		Avena sativa	-	third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA	390773	-	390773	?
12-oxo-cis-phythodienoic acid + NADPH	cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+		Helianthus annuus	-	third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA	390772	-	-	?
12-oxo-cis-phythodienoic acid + NADPH	cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+		Helianthus annuus, Solanum melongena, Linum usitatissimum	-	third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA	390773	-	390773	?
12-oxo-cis-phythodienoic acid + NADPH	cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+		Arabidopsis sp.	-	enzyme induced upon wounding	390778	OPC-8:0	390778	-
12-oxo-cis-phythodienoic acid + NADPH	cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+		Arabidopsis thaliana	-	wound-induced gene activation	390778	OPC-8:0	390778	?
12-oxo-cis-phythodienoic acid + NADPH	cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+		Arabidopsis thaliana	-	enzyme of the octadecanoid pathway leading to jasmonic acid	390777	-	-	?
12-oxo-phytodienoic acid + NADPH	3-oxo-2-(2'(Z)-pentenyl)-cyclopentane-1 octanoic acid + NADP+		Oryza sativa	Q8H9F1	enzyme catalyzes a late step in the jasmonic acid biosynthetic pathway	690761	-	-	?
12-oxo-trans-phythodienoic acid + alpha-NADPH	trans-3-oxo-2-(2'-pentenyl)cyclopentaneoctanoic acid + NADP+		Arabidopsis sp.	-	-	390778	-	-	?
12-oxo-trans-phythodienoic acid + beta-NADPH	trans-3-oxo-2-(2'-pentenyl)cyclopentaneoctanoic acid + NADP+		Arabidopsis sp.	-	-	390778	-	-	?
12-oxo-trans-phythodienoic acid + NADPH	t-3-oxo-2-(2'-pentenyl)cyclopentaneoctanoic acid + NADP+		Zea mays	-	-	390774, 390776	-	-	?
12-oxo-trans-phythodienoic acid + NADPH	t-3-oxo-2-(2'-pentenyl)cyclopentaneoctanoic acid + NADP+		Corydalis sempervirens	-	-	390776	-	-	?
15-deoxy-DELTA12,14-prostaglandin J2 + alpha-NADPH	? + alpha-NADPH		Arabidopsis sp.	-	PGJ2, an analogue of OPDA, rates of reaction with alpha-NADPH twenty times lower than those with beta-NADPH	390778	-	-	?
15-deoxy-DELTA12,14-prostaglandin J2 + beta-NADPH	? + NADP+		Arabidopsis sp.	-	PGJ2, an analogue of OPDA	390778	-	-	?
2,6,6-trimethylcyclohex-2-ene-1,4-dione + NADH	(6R)-2,2,6-trimethylcyclohexane-1,4-dione + NAD+		Solanum lycopersicum	Q9FEW9, Q9XG54	77% conversion with OPR-3	684429	-	-	?
2,6,6-trimethylcyclohex-2-ene-1,4-dione + NADH	(6R)-2,2,6-trimethylcyclohexane-1,4-dione + NAD+		Solanum lycopersicum	Q9FEW9, Q9XG54	above 98% conversion with OPR-1	684429	-	-	?
2,6,6-trimethylcyclohex-2-ene-1,4-dione + NADPH	(6R)-2,2,6-trimethylcyclohexane-1,4-dione + NADP+		Solanum lycopersicum	Q9FEW9, Q9XG54	78% conversion with OPR-3	684429	-	-	?
2,6,6-trimethylcyclohex-2-ene-1,4-dione + NADPH	(6R)-2,2,6-trimethylcyclohexane-1,4-dione + NADP+		Solanum lycopersicum	Q9FEW9, Q9XG54	above 98% conversion with OPR-1	684429	-	-	?
2-cyclohexen-1-one + NADH	? + NAD+		Pseudomonas putida	-	-	678118	-	-	?
2-cyclohexen-1-one + NADPH	? + NADP+		Pisum sativum	Q9AVK9	best substrate	656976	-	-	?
2-cyclohexen-1-one + NADPH	? + NADP+		Pisum sativum	Q76FR7, Q76FR8, Q76FR9, Q76FS0, Q76FS1, Q9AVK9	model substrate, isozyme OPR3	656802	-	-	?
2-cyclohexen-1-one + NADPH	? + NADP+		Pisum sativum	Q76FR7, Q76FR8, Q76FR9, Q76FS0, Q76FS1, Q9AVK9	model substrate, isozyme OPR4	656802	-	-	?
2-cyclohexen-1-one + NADPH	? + NADP+		Pisum sativum	Q76FR7, Q76FR8, Q76FR9, Q76FS0, Q76FS1, Q9AVK9	model substrate, isozyme OPR6	656802	-	-	?
2-cyclohexen-1-one + NADPH	? + NADP+		Pisum sativum	Q76FR7, Q76FR8, Q76FR9, Q76FS0, Q76FS1, Q9AVK9	model substrate, isozymes OPR1	656802	-	-	?
2-cyclohexen-1-one + NADPH	? + NADP+		Pisum sativum	Q76FR7, Q76FR8, Q76FR9, Q76FS0, Q76FS1, Q9AVK9	model substrate, isozymes OPR2	656802	-	-	?
2-methylcyclopent-2-en-1-one + NADH	(2S)-2-methylcyclopentanone + NAD+		Solanum lycopersicum	Q9FEW9, Q9XG54	19% conversion with OPR-3	684429	-	-	?
2-methylcyclopent-2-en-1-one + NADH	(2S)-2-methylcyclopentanone + NAD+		Solanum lycopersicum	Q9FEW9, Q9XG54	58% conversion with OPR-1	684429	-	-	?
2-methylcyclopent-2-en-1-one + NADPH	(2S)-2-methylcyclopentanone + NADP+		Solanum lycopersicum	Q9FEW9, Q9XG54	27% conversion with OPR-3	684429	-	-	?
2-methylcyclopent-2-en-1-one + NADPH	(2S)-2-methylcyclopentanone + NADP+		Solanum lycopersicum	Q9FEW9, Q9XG54	45% conversion with OPR-1	684429	-	-	?
3-methyl-1-phenyl-1H-pyrrole-2,5-dione + NADH	(3R)-3-methyl-1-phenylpyrrolidine-2,5-dione + NAD+		Solanum lycopersicum	Q9FEW9, Q9XG54	99% conversion with OPR-1	684429	-	-	?
3-methyl-1-phenyl-1H-pyrrole-2,5-dione + NADH	(3R)-3-methyl-1-phenylpyrrolidine-2,5-dione + NAD+		Solanum lycopersicum	Q9FEW9, Q9XG54	99% conversion with OPR-3	684429	-	-	?
3-methyl-1-phenyl-1H-pyrrole-2,5-dione + NADPH	(3R)-3-methyl-1-phenylpyrrolidine-2,5-dione + NADP+		Solanum lycopersicum	Q9FEW9, Q9XG54	99% conversion with OPR-1	684429	-	-	?
3-methyl-1-phenyl-1H-pyrrole-2,5-dione + NADPH	(3R)-3-methyl-1-phenylpyrrolidine-2,5-dione + NADP+		Solanum lycopersicum	Q9FEW9, Q9XG54	99% conversion with OPR-3	684429	-	-	?
3-methylcyclopent-2-en-1-one + NADH	(3S)-3-methylcyclopentanone + NAD+		Solanum lycopersicum	Q9FEW9, Q9XG54	2% conversion with OPR-3	684429	-	-	?
3-methylcyclopent-2-en-1-one + NADPH	(3S)-3-methylcyclopentanone + NADP+		Solanum lycopersicum	Q9FEW9, Q9XG54	1% conversion with OPR-3	684429	-	-	?
3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+	?		Solanum lycopersicum	-	in vitro, OPR3 reduces the jasmonic acid precursor (9S,13S)-12-oxophytodienoate as well as the enantiomeric (9R,13R)-12-oxophytodienoate, while its isozyme OPR1 is highly selective, accepting only (9R,13R)-12-oxophytodienoate as a substrate, the enzyme in plants relates to old yellow enzyme (OYE) from Saccharomyces cerevisiae	699588	-	-	-
9R,13R-12-oxo-phytodienoic acid + NADPH	? + NADP+		Arabidopsis sp.	-	oxylipin	390778	-	-	?
9S,13R-12-oxo-phytodienoic acid + NADPH	? + NADP+		Arabidopsis thaliana	-	does not utilize 13S-configured isomers	390776	-	-	?
beta-oestradiol + NADPH	? + NADP+		Saccharomyces cerevisiae	-	-	390778	-	-	?
beta-oestradiol + NADPH	? + NADP+		Arabidopsis sp.	-	very low but significant oxidoreductase activity, detected in micromolar quantities	390778	-	-	?
codeinone + NADH	hydrocodone + NAD+		Pseudomonas putida	-	-	678118	-	-	?
cyclohexenone + alpha-NADPH	? + NADP+		Arabidopsis sp.	-	-	390778	-	-	?
morphinone + NADH	hydromorphone + NAD+		Pseudomonas putida	-	-	678118	-	-	?
oxylipin + NADPH	? + NADP+		Solanum lycopersicum	-	-	390780	-	-	?
testosterone + NADPH	? + NADP+		Saccharomyces cerevisiae	-	-	390778	-	-	?
trans-hex-2-enal + NADPH	hexanal + NADP+		Solanum lycopersicum	Q9FEW9	-	676813	-	-	?
[(E)-1-methyl-2-nitroethenyl]benzene + NADH	[(1R)-1-methyl-2-nitroethyl]benzene + NAD+		Solanum lycopersicum	Q9FEW9, Q9XG54	above 99% conversion with OPR-1	684429	-	-	?
[(E)-1-methyl-2-nitroethenyl]benzene + NADH	[(1S)-1-methyl-2-nitroethyl]benzene + NAD+		Solanum lycopersicum	Q9FEW9, Q9XG54	69% conversion with OPR-3	684429	-	-	?
[(E)-1-methyl-2-nitroethenyl]benzene + NADPH	[(1R)-1-methyl-2-nitroethyl]benzene + NADP+		Solanum lycopersicum	Q9FEW9, Q9XG54	above 99% conversion with OPR-1	684429	-	-	?
[(E)-1-methyl-2-nitroethenyl]benzene + NADPH	[(1S)-1-methyl-2-nitroethyl]benzene + NADP+		Solanum lycopersicum	Q9FEW9, Q9XG54	72% conversion with OPR-3	684429	-	-	?
cyclohexenone + beta-NADPH	?		Saccharomyces cerevisiae, Arabidopsis sp.	-	-	390778	-	-	?
additional information	?	-	Arabidopsis sp.	-	cyclohexanone, testosterone and progesterone are no substrates	390778	-	-	-
additional information	?	-	Oryza sativa	Q8H9F1	physiological role of the enzyme	657134	-	-	-
additional information	?	-	Solanum lycopersicum	Q9FEW9, Q9FEX0, Q9XG54	phylogenetic analysis	656998	-	-	-
additional information	?	-	Pisum sativum	Q76FR7, Q76FR8, Q76FR9, Q76FS0, Q76FS1, Q9AVK9	isozyme belongs to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis	656802	-	-	-
additional information	?	-	Pisum sativum	Q76FR7, Q76FR8, Q76FR9, Q76FS0, Q76FS1, Q9AVK9	isozymes belong to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis	656802	-	-	-
additional information	?	-	Solanum lycopersicum	Q9FEW9, Q9FEX0, Q9XG54	phylogenetic analysis, enzyme is involved in octadecanoid biosynthesis which has differential roles for in the local versus the systemic wound response	656998	-	-	-
additional information	?	-	Arabidopsis thaliana	-	phylogenetic analysis, isozyme OPR3 is induced in self-defense signalling, enzyme is involved in octadecanoid and jasmonate biosynthesis with differential roles for in the local versus the systemic wound response, cDNA micro array analysis	656998	-	-	-
additional information	?	-	Pisum sativum	Q9AVK9	suppressor-inducible enzyme activity leads to jasmonate biosynthesis or related compound, which might contribute to compatibility by specific activation of the octadecanoid pathway and inhibition of the phenylpropanoid biosynthetic pathway	656976	-	-	-
additional information	?	-	Pisum sativum	Q76FR7, Q76FR8, Q76FR9, Q76FS0, Q76FS1, Q9AVK9	isozyme OPR5 is inactive	656802	-	-	-
additional information	?	-	Solanum lycopersicum	Q9FEW9, Q9FEX0, Q9XG54	no activity of isozyme OPR2 with (15Z)-12-oxophyto-10,15-dienoate stereoisomers	656998	-	-	-
additional information	?	-	Arabidopsis thaliana	-	stereospecificity of isozymes, overview, isozyme OPR1 shows a broad substrate specificity reducing a wide range of alpha,beta-unsaturated carbonyl compounds	656998	-	-	-
additional information	?	-	Solanum lycopersicum	Q9FEW9, Q9FEX0, Q9XG54	stereospecificity of isozymes, overview, isozyme OPR1 shows a broad substrate specificity reducing a wide range of alpha,beta-unsaturated carbonyl compounds	656998	-	-	-
additional information	?	-	Solanum lycopersicum	Q9FEW9, Q9FEX0, Q9XG54	stereospecificity of isozymes, overview, isozyme OPR3 shows a broad substrate specificity reducing a wide range of alpha,beta-unsaturated carbonyl compounds	656998	-	-	-
additional information	?	-	Solanum lycopersicum	Q9FEW9, Q9XG54	isozyme OPR-1 does not convert 3-methylcyclopent-2-en-1-one	684429	-	-	-
additional information	?	-	Solanum lycopersicum	Q9FEW9, Q9XG54	isozyme OPR-3 does not convert (2Z)-2-methylbut-2-enedioic acid	684429	-	-	-
additional information	?	-	Solanum lycopersicum	-	OPR1-like enzymes are highly selective, accepting only (9R,13R)-12-oxophytodienoate as a substrate	699588	-	-	-
additional information	?	-	Arabidopsis thaliana	Q8GYB8, Q8LAH7, Q9FUP0	OPR1 is able to transform 2,4,6-trinitrotoluene to yield nitro-reduced derivatives, it additionally produces the aromatic ring-reduced products hydride and dihydride Meisenheimer complexes	700835	-	-	-
additional information	?	-	Arabidopsis thaliana	Q8GYB8, Q8LAH7, Q9FUP0	OPR2 is able to transform 2,4,6-trinitrotoluene to yield nitro-reduced derivatives	700835	-	-	-
additional information	?	-	Arabidopsis thaliana	Q8GYB8, Q8LAH7, Q9FUP0	OPR3 is able to transform 2,4,6-trinitrotoluene to yield nitro-reduced derivatives	700835	-	-	-

NATURAL SUBSTRATES	NATURAL PRODUCTS	REACTION DIAGRAM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY SUBSTRATE	LITERATURE (Substrate)	COMMENTARY PRODUCT	LITERATURE (Product)
(15Z)-12-oxophyto-10,15-dienoate + NADPH	8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+		Arabidopsis thaliana	-	-	656998	-	-
(15Z)-12-oxophyto-10,15-dienoate + NADPH	8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+		Solanum lycopersicum	Q9FEW9, Q9FEX0, Q9XG54	-	656998	-	-
(15Z)-12-oxophyto-10,15-dienoate + NADPH	8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+		Oryza sativa	Q8H9F1	leads to jasmonate biosynthesis	657134	-	-
(9R,13R)-(15Z)-12-oxophyto-10,15-dienoate + NADPH	(9R,13R)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+		Solanum lycopersicum	Q9FEW9, Q9FEX0, Q9XG54	-	656998	-	-
(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH	(9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+		Arabidopsis thaliana	-	product is an intermediate in the jasmonate biosynthesis	657324	-	-
(9S,13S)-(15Z)-12-oxophyto-10,15-dienoate + NADPH	(9S,13S)-8-[(1R,2R)-3-oxo-2-((Z)-pent-2-enyl)cyclopentyl]octanoate + NADP+		Solanum lycopersicum	Q9FEW9, Q9FEX0, Q9XG54	product is the direct precursor in the jasmonate biosynthesis	656998	-	-
(9S,13S)-12-oxo-phytodienoic acid + NADPH	3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+		Arabidopsis thaliana	-	-	390776	-	-
(9S,13S)-12-oxo-phytodienoic acid + NADPH	3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+		Arabidopsis thaliana	-	OPR3 is the isoenzyme relevant for jasmonate biosynthesis	390779	-	-
(9S,13S)-12-oxo-phytodienoic acid + NADPH	3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+		Corydalis sempervirens	-	OPRII reduces all 4 OPDA isomers	390776	-	-
(9S,13S)-12-oxo-phytodienoic acid + NADPH	3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+		Corydalis sempervirens	-	reductase specific for the natural cis-OPDA enatiomer encoded by OPR2	390779	-	-
(9S,13S)-12-oxo-phytodienoic acid + NADPH + H+	3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+		Solanum lycopersicum	-	enzyme is a FMN-dependent oxidoreductase that catalyzes the reduction of the cyclopentenone (9S,13S)-12-oxophytodienoate to the corresponding cyclopentanone in the biosynthesis of the plant hormone jasmonic acid	699588	-	-
(9S,13S)-12-oxo-phytodienoic acid + NADPH + H+	?		Solanum lycopersicum	-	FMN-dependent oxidoreductase, biosynthesis of the plant hormone jasmonic acid, the enzyme in plants relates to old yellow enzyme (OYE) from Saccharomyces cerevisiae	699588	-	-
(9S,13S)-12-oxophytodienoic acid + NADPH + H+	3-2(2'(Z)-pentyl)cyclopentane-1-octanoic acid + NADP+		Arabidopsis thaliana	Q8GYB8, Q8LAH7, Q9FUP0	OPR3 is involved in jasmonic acid biosynthesis	700835	-	-
12-oxo-cis-phythodienoic acid + NADPH	cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+		Zea mays	-	-	390778	-	390778
12-oxo-cis-phythodienoic acid + NADPH	cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+		Triticum aestivum	-	third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA	390773	-	390773
12-oxo-cis-phythodienoic acid + NADPH	cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+		Zea mays	-	third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA	390772	-	-
12-oxo-cis-phythodienoic acid + NADPH	cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+		Zea mays	-	third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA	390773	-	390773
12-oxo-cis-phythodienoic acid + NADPH	cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+		Zea mays	-	third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA	390774	-	390774
12-oxo-cis-phythodienoic acid + NADPH	cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+		Avena sativa	-	third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA	390773	-	390773
12-oxo-cis-phythodienoic acid + NADPH	cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+		Helianthus annuus	-	third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA	390772	-	-
12-oxo-cis-phythodienoic acid + NADPH	cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+		Helianthus annuus, Solanum melongena, Linum usitatissimum	-	third enzyme of the biosynthesis pathway of jasmonic acid from linolenic acid, enzyme catalyzes the reduction of a double bond in the cyclopentenone ring of 12-oxo-PDA	390773	-	390773
12-oxo-cis-phythodienoic acid + NADPH	cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+		Arabidopsis sp.	-	enzyme induced upon wounding	390778	OPC-8:0	390778
12-oxo-cis-phythodienoic acid + NADPH	cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+		Arabidopsis thaliana	-	wound-induced gene activation	390778	OPC-8:0	390778
12-oxo-cis-phythodienoic acid + NADPH	cis-3-oxo-2-(2'-pentenyl)cyclopentane-1-octanoic acid + NADP+		Arabidopsis thaliana	-	enzyme of the octadecanoid pathway leading to jasmonic acid	390777	-	-
12-oxo-phytodienoic acid + NADPH	3-oxo-2-(2'(Z)-pentenyl)-cyclopentane-1 octanoic acid + NADP+		Oryza sativa	Q8H9F1	enzyme catalyzes a late step in the jasmonic acid biosynthetic pathway	690761	-	-
3-oxo-2((2Z)-pentenyl)-cyclopentane-1-octanoic acid + NADP+	?		Solanum lycopersicum	-	in vitro, OPR3 reduces the jasmonic acid precursor (9S,13S)-12-oxophytodienoate as well as the enantiomeric (9R,13R)-12-oxophytodienoate, while its isozyme OPR1 is highly selective, accepting only (9R,13R)-12-oxophytodienoate as a substrate, the enzyme in plants relates to old yellow enzyme (OYE) from Saccharomyces cerevisiae	699588	-	-
9R,13R-12-oxo-phytodienoic acid + NADPH	? + NADP+		Arabidopsis sp.	-	oxylipin	390778	-	-
oxylipin + NADPH	? + NADP+		Solanum lycopersicum	-	-	390780	-	-
9S,13R-12-oxo-phytodienoic acid + NADPH	? + NADP+		Arabidopsis thaliana	-	does not utilize 13S-configured isomers	390776	-	-
additional information	?	-	Oryza sativa	Q8H9F1	physiological role of the enzyme	657134	-	-
additional information	?	-	Solanum lycopersicum	Q9FEW9, Q9FEX0, Q9XG54	phylogenetic analysis	656998	-	-
additional information	?	-	Pisum sativum	Q76FR7, Q76FR8, Q76FR9, Q76FS0, Q76FS1, Q9AVK9	isozyme belongs to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis	656802	-	-
additional information	?	-	Pisum sativum	Q76FR7, Q76FR8, Q76FR9, Q76FS0, Q76FS1, Q9AVK9	isozymes belong to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis	656802	-	-
additional information	?	-	Solanum lycopersicum	Q9FEW9, Q9FEX0, Q9XG54	phylogenetic analysis, enzyme is involved in octadecanoid biosynthesis which has differential roles for in the local versus the systemic wound response	656998	-	-
additional information	?	-	Arabidopsis thaliana	-	phylogenetic analysis, isozyme OPR3 is induced in self-defense signalling, enzyme is involved in octadecanoid and jasmonate biosynthesis with differential roles for in the local versus the systemic wound response, cDNA micro array analysis	656998	-	-
additional information	?	-	Pisum sativum	Q9AVK9	suppressor-inducible enzyme activity leads to jasmonate biosynthesis or related compound, which might contribute to compatibility by specific activation of the octadecanoid pathway and inhibition of the phenylpropanoid biosynthetic pathway	656976	-	-
additional information	?	-	Solanum lycopersicum	-	OPR1-like enzymes are highly selective, accepting only (9R,13R)-12-oxophytodienoate as a substrate	699588	-	-

COFACTOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
Flavin	Arabidopsis thaliana	-	dependent on	656998	2D-image
Flavin	Solanum lycopersicum	Q9FEW9, Q9FEX0, Q9XG54	dependent on	656998	2D-image
Flavin	Pseudomonas putida	-	dependent, contains one molar equivalent of noncovalently bound FMN per enzyme subunit	678118	2D-image
FMN	Arabidopsis thaliana	-	one molecule per enzyme monomer	677040	2D-image
NADH	Pseudomonas putida	-	dependent	678118	2D-image
NADH	Solanum lycopersicum	-	;	684429	2D-image
NADP+	Solanum lycopersicum	-	-	699588	2D-image
NADPH	Zea mays	-	preferred reductant	390774	2D-image
NADPH	Pisum sativum	-	-	656802, 656976	2D-image
NADPH	Arabidopsis thaliana	-	-	656998, 657324	2D-image
NADPH	Solanum lycopersicum	Q9FEW9, Q9FEX0, Q9XG54	-	656998	2D-image
NADPH	Oryza sativa	-	-	657134, 689685	2D-image
NADPH	Solanum lycopersicum	Q9FEW9, Q9XG54	;	684429, 699588	2D-image
NADPH	Zea mays	Q49HD7, Q49HD8, Q49HD9, Q49HE0, Q49HE1, Q49HE2, Q49HE3	; ; ; ; ; ; ;	689081	2D-image
NADPH	Zea mays	-	-	689661	2D-image
FMN	Solanum lycopersicum	-	;	699588	2D-image
additional information	Solanum lycopersicum	Q9FEW9	in the dimer interface of enzyme, a sulfate ion is bound which forms hydrogen bonds with two arginines of protomer A and one arginine of loop L6 of the partner protomer B. Sulfate ion may mimic the phosphate group of phosphorylated T364	676813	-

METALS and IONS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
Mg2+	Arabidopsis thaliana	-	-	656998
Mg2+	Solanum lycopersicum	Q9FEW9, Q9FEX0, Q9XG54	-	656998

INHIBITORS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
cortisone	Pseudomonas putida	-	-	678118	2D-image
p-Hydroxybenzaldehyde	Solanum lycopersicum	-	;	699588	2D-image
progesterone	Pseudomonas putida	-	-	678118	2D-image

ACTIVATING COMPOUND	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
2,4,6-trinitrotoluene	Arabidopsis thaliana	Q8GYB8, Q8LAH7, Q9FUP0	expression of oxophytodienoate reductases increases in response to the explosive 2,4,6-trinitrotoluene, 17fold up-regulation of OPR1 in the root; expression of oxophytodienoate reductases increases in response to the explosive 2,4,6-trinitrotoluene, 6fold up-regulation of OPR2 in the root	700835	2D-image
cis-3-hexen-1-yl acetate	Zea mays	Q49HD7, Q49HD8, Q49HD9, Q49HE0, Q49HE1, Q49HE2, Q49HE3	OPR activity almost doubles in cis-3-hexen-1-yl acetate-exposed plants; OPR activity almost doubles in cis-3-hexen-1-yl acetate-exposed plants; OPR activity almost doubles in cis-3-hexen-1-yl acetate-exposed plants; OPR activity almost doubles in cis-3-hexen-1-yl acetate-exposed plants; OPR activity almost doubles in cis-3-hexen-1-yl acetate-exposed plants; OPR activity almost doubles in cis-3-hexen-1-yl acetate-exposed plants; OPR activity almost doubles in cis-3-hexen-1-yl acetate-exposed plants; OPR activity almost doubles in cis-3-hexen-1-yl acetate-exposed plants	689081	2D-image
additional information	Pisum sativum	Q76FR7, Q76FR8, Q76FR9, Q76FS0, Q76FS1, Q9AVK9	isozyme OPR1 is induced by coronatine; isozyme OPR2, i.e. S64, expression is induced by substrate, methyljasmonate, or compatible pathogens, or a suppressor derived from fungus Mycosphaerella pinodes, isozyme OPR2 is induced by coronatine; isozyme OPR4 is induced by coronatine; isozyme OPR6 is induced by coronatine; isozymes OPR3 is slightly induced by coronatine	656802	-
additional information	Pisum sativum	Q9AVK9	enzyme expression is inducible by regulatory suppressor glycopeptides from the fungus Mycosphaerella pinodes, which block the pea defense response induced by elicitors	656976	-
additional information	Zea mays	-	OPR1 is induced by osmotic stress as well as high salt	689661	-

KM VALUE [mM]	KM VALUE [mM] Maximum	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
0.043	-	(15Z)-12-oxophyto-10,15-dienoate	Pisum sativum	Q9AVK9	pH 7.5, 25�C, recombinant enzyme	656976	2D-image
0.035	-	(9S,13S)-12-oxophytodienoic acid	Arabidopsis thaliana	-	-	390779	2D-image
0.045	-	12-oxo-cis-phythodienoic acid	Arabidopsis sp.	-	-	390778	2D-image
0.19	-	12-oxophytodienoic acid	Zea mays	-	-	390774	2D-image
0.055	-	2-cyclohexen-1-one	Pisum sativum	Q9AVK9	pH 7.5, 25�C, recombinant enzyme	656976	2D-image
2.5	-	cyclohexenone	Arabidopsis sp.	-	-	390778	2D-image
4.2	-	NADH	Zea mays	-	-	390774	2D-image
0.013	-	NADPH	Zea mays	-	-	390774	2D-image

TURNOVER NUMBER [1/s]	TURNOVER NUMBER MAXIMUM[1/s]	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
14	-	trans-hex-2-enal	Solanum lycopersicum	Q9FEW9	-	676813	2D-image
additional information	-	additional information	Solanum lycopersicum	Q9FEW9	for oxidative half-reaction, kox of trans-hex-2-2enal is 0.3 per sec	676813	-

kcat/KM VALUE [1/mMs^-1]	kcat/KM VALUE [1/mMs^-1] Maximum	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

Ki VALUE [mM]	Ki VALUE [mM] Maximum	INHIBITOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

IC50 VALUE [mM]	IC50 VALUE [mM] Maximum	INHIBITOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg]	SPECIFIC ACTIVITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
additional information	-	Pisum sativum	Q9AVK9	-	656976
additional information	-	Oryza sativa	Q8H9F1	jasmonic acid deficiency in OPR-interfering RNA plants does not influence resistance to Magnaporthe grisea or the pathogenesis-related gene expressions in these plants; jasmonic acid deficiency in OPR-interfering RNA plants does not influence resistance to Magnaporthe grisea or the pathogenesis-related gene expressions in these plants	690761
additional information	-	Arabidopsis thaliana	Q8GYB8, Q8LAH7, Q9FUP0	OPR1-overexpressing lines have seedling root lengths significantly longer than those of wild-type plants when growing on solid medium containing 2 microM 2,4,6-trinitrotoluene, enhanced tolerance to 2,4,6-trinitrotoluene agrees with that observed in transgenic tobacco, OPR1-overexpressing lines 1.1 and 9.4 show enhanced 2,4,6-trinitrotoluene uptake; OPR2-overexpressing lines have seedling root lengths significantly longer than those of wild-type plants when growing on solid medium containing 2 microM 2,4,6-trinitrotoluene, enhanced tolerance to 2,4,6-trinitrotoluene agrees with that observed in transgenic tobacco	700835

pH OPTIMUM	pH MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
6.8	8	Zea mays	-	-	390774
7.5	8	Corydalis sempervirens	-	OPRII	390776
7.5	-	Pisum sativum	Q76FR7, Q76FR8, Q76FR9, Q76FS0, Q76FS1, Q9AVK9	assay at	656802
7.5	-	Pisum sativum	Q9AVK9	assay at	656976
7.5	-	Arabidopsis thaliana	-	assay at	656998
7.5	-	Solanum lycopersicum	Q9FEW9, Q9FEX0, Q9XG54	assay at	656998
7.5	-	Oryza sativa	Q8H9F1	assay at	657134
7.5	-	Solanum lycopersicum	-	assay at; assay at	699588
8.7	-	Corydalis sempervirens	-	OPRI	390776

pH RANGE	pH RANGE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
5	8.5	Arabidopsis sp.	-	-	390778
5	9	Zea mays	-	-	390774

TEMPERATURE OPTIMUM	TEMPERATURE OPTIMUM MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
25	-	Pisum sativum	Q76FR7, Q76FR8, Q76FR9, Q76FS0, Q76FS1, Q9AVK9	assay at	656802
25	-	Pisum sativum	Q9AVK9	assay at	656976
25	-	Arabidopsis thaliana	-	assay at	656998
25	-	Solanum lycopersicum	Q9FEW9, Q9FEX0, Q9XG54	assay at	656998
25	-	Oryza sativa	Q8H9F1	assay at	657134

TEMPERATURE RANGE	TEMPERATURE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

pI VALUE	pI VALUE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

SOURCE TISSUE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	SOURCE
cell suspension culture	Oryza sativa	Q8H9F1	-	657134
cotyledon	Helianthus annuus, Linum usitatissimum	-	-	390773
epicotyl	Pisum sativum	-	-	656802, 656976
flower	Arabidopsis thaliana	-	-	390777
fruit	Solanum melongena	-	-	390773
kernel	Zea mays	-	-	390774
leaf	Avena sativa, Triticum aestivum	-	-	390773
leaf	Arabidopsis thaliana	-	-	390777, 390778
leaf	Arabidopsis sp.	-	-	390778
leaf	Pisum sativum	-	-	656802, 656976
leaf	Arabidopsis thaliana	-	isozyme OPR3, up-regulation in wounded leaves, low activity in unwounded, systemic leaves	656998
leaf	Zea mays	-	hardly observed expression in leaves	689661
leaf	Oryza sativa	-	-	689685
root	Arabidopsis sp.	-	OPR1 and OPR2, OPR3 poorly expressed	390778
root	Zea mays	-	-	689661
root	Arabidopsis thaliana	Q8GYB8, Q8LAH7, Q9FUP0	;	700835
seedling	Zea mays	-	-	390772, 390773, 390774, 390778, 689661
seedling	Solanum melongena	-	-	390773
seedling	Arabidopsis sp.	-	-	390778
seedling	Arabidopsis thaliana	Q8GYB8, Q8LAH7, Q9FUP0	OPR overexpressing line; OPR overexpressing line; OPR overexpressing line	700835
shoot	Arabidopsis thaliana	-	-	656998
tendril	Bryonia dioica	-	-	390777
mesophyll	Arabidopsis thaliana	-	-	656998
additional information	Pisum sativum	Q76FR7, Q76FR8, Q76FR9, Q76FS0, Q76FS1, Q9AVK9	isozyme gene expression profiles	656802

LOCALIZATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	GeneOntology No.	LITERATURE	SOURCE
chloroplast	Corydalis sempervirens	-	-	9507	390776
cytosol	Corydalis sempervirens	-	-	5829	390776
cytosol	Arabidopsis thaliana	-	isozymes OPR1 and OPR2	5829	656998
cytosol	Solanum lycopersicum	Q9FEW9, Q9FEX0, Q9XG54	isozyme OPR1; isozyme OPR2	5829	656998
peroxisome	Arabidopsis thaliana	-	isozyme OPR3	5777	656998
peroxisome	Solanum lycopersicum	Q9FEW9, Q9FEX0, Q9XG54	isozyme OPR3	5777	656998
peroxisome	Arabidopsis thaliana	Q8GYB8, Q8LAH7, Q9FUP0	OPR3	5777	700835

PDB	SCOP	CATH	ORGANISM
1q45, download	SCOP (1q45)	CATH (1q45)	Arabidopsis thaliana
2g5w, download	SCOP (2g5w)	CATH (2g5w)	Arabidopsis thaliana
2q3o, download	SCOP (2q3o)	CATH (2q3o)	Arabidopsis thaliana
2q3r, download	SCOP (2q3r)	CATH (2q3r)	Arabidopsis thaliana
1icp, download	SCOP (1icp)	CATH (1icp)	Solanum lycopersicum
1icq, download	SCOP (1icq)	CATH (1icq)	Solanum lycopersicum
1ics, download	SCOP (1ics)	CATH (1ics)	Solanum lycopersicum
2hs6, download	SCOP (2hs6)	CATH (2hs6)	Solanum lycopersicum
2hs8, download	SCOP (2hs8)	CATH (2hs8)	Solanum lycopersicum
2hsa, download	SCOP (2hsa)	CATH (2hsa)	Solanum lycopersicum
3hgo, download	SCOP (3hgo)	CATH (3hgo)	Solanum lycopersicum
3hgr, download	SCOP (3hgr)	CATH (3hgr)	Solanum lycopersicum
3hgs, download	SCOP (3hgs)	CATH (3hgs)	Solanum lycopersicum

MOLECULAR WEIGHT	MOLECULAR WEIGHT MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
41170	-	Arabidopsis thaliana	Q8LAH7	predicted from amino acid sequence	390775
42000	-	Corydalis sempervirens	-	OPRI, gel filtration	390776
42500	-	Corydalis sempervirens	-	OPRII, gel filtration	390776
54000	-	Zea mays	-	gel filtration	390774
82200	-	Pseudomonas putida	-	-	678118

SUBUNITS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
?	Oryza sativa	Q8H9F1	x * 41000, recombinant His-tagged enzyme, SDS-PAGE	657134
dimer	Saccharomyces cerevisiae	-	homodimeric protein with one molecule of flavin per monomer	390778
dimer	Solanum lycopersicum	Q9FEW9	concentration-dependent shift from monomer with 40000 Da at 0.022 mM to dimer with 80000 Da at 0.358 mM, dynamic light scattering	676813
dimer	Solanum lycopersicum	-	OPR3 crystallizes as a self-inhibited dimer	699588
homodimer	Pseudomonas putida	-	2 * 41100	678118
monomer	Corydalis sempervirens	-	1 * 42000, OPRI, SDS-PAGE; 1 * 42500, OPRII, SDS-PAGE	390776
monomer	Solanum lycopersicum	-	-	390780
monomer	Solanum lycopersicum	Q9FEW9	concentration-dependent shift from monomer with 40000 Da at 0.022 mM to dimer with 80000 Da at 0.358 mM, dynamic light scattering	676813
additional information	Solanum lycopersicum	Q9FEW9	rapid monomer-dimer equilibrium with a high dissociation constant in vitro	676813

POSTTRANSLATIONAL MODIFICATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

Crystallization/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
10 mg/ml purified recombinant isozyme OPR3 in 100 mM MES, pH 6.0, 100 mM NaCl, 0.3 mM TCEP, hanging drop vapour diffusion method, 0.002 ml protein solution with equal volume of well solution containing 8.75-10.0% monomethyl ether polyethylene glycol 5000, 0.1 M triethanolamine, pH 8.0, 0.275-0.35 M glycine, crystals are harvested into a cryoprotectant solution containing 12% monomethyl ether polyethylene glycol 5000, 0.1 M triethanolamine, pH 8.0, 0.4 M glycine, and 25% PEG 400, X-ray diffraction structure determination and analysis at 2.0 A resolution, molecular replacement	Arabidopsis thaliana	-	657324
one enzyme monomer per asymmetric unit, space group C2221. Enzyme is a member of an alpha/beta barrel fold family of FMN-containing oxidoreductases	Arabidopsis thaliana	-	677040
-	Saccharomyces cerevisiae	-	390778
at 2.0 A resolution, determination of the crystal structures of OPR3 in complex with the ligand p-hydroxybenzaldehyde, structural comparison with the OPR1:(9R,13R)-12-oxophytodienoate complex and further biochemical and mutational analyses reveals that 2 active-site residues, Phe74 and His244 in OPR3 are critical for substrate filtering; OPR1:p-hydroxybenzaldehyde complex at 2.3 A resolution, determination of the crystal structures of OPR1 in complex with the ligand p-hydroxybenzaldehyde, structural comparison with the OPR1:(9R,13R)-12-oxophytodienoate complex and further biochemical and mutational analyses reveals that 2 active-site residues, Tyr78 and Tyr246 in OPR1 are critical for substrate filtering	Solanum lycopersicum	-	699588
native enzyme and mutants E291L and Y364F. Wild-type enzyme crystallizes as an extraordinary self-inhibiting dimer, dimerization is actively driven by the mutual binding of the two L6 loops into the two active sites	Solanum lycopersicum	Q9FEW9	676813
OPR1	Solanum lycopersicum	-	390780

pH STABILITY	pH STABILITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

TEMPERATURE STABILITY	TEMPERATURE STABILITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

GENERAL STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

ORGANIC SOLVENT	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

OXIDATION STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

STORAGE STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

Purification/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
recombinant protein	Arabidopsis sp.	-	390778
by affinity chromatography; by affinity chromatography; by affinity chromatography	Arabidopsis thaliana	Q8GYB8, Q8LAH7, Q9FUP0	700835
OPR1, OPR2 and OPR3	Arabidopsis thaliana	-	390779
recombinant isozyme OPR3 from Escherichia coli by nickel-imino-diacetic acid affinity chromatography	Arabidopsis thaliana	-	657324
recombinant tagged isozymes OPR1-3 from Escherichia coli by affinity chromatography	Arabidopsis thaliana	-	656998
-	Corydalis sempervirens	-	390776, 390779
purified in enzymatically active form	Corydalis sempervirens	-	390775
recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography	Oryza sativa	Q8H9F1	657134
recombinant isozyme from Escherichia coli	Pisum sativum	Q76FR7, Q76FR8, Q76FR9, Q76FS0, Q76FS1, Q9AVK9	656802
-	Saccharomyces cerevisiae	-	390776, 390779
recombinant His-tagged isozyme OPR1 from Escherichia coli by nickel affinity chromatography; recombinant His-tagged isozyme OPR2 from Escherichia coli by nickel affinity chromatography; recombinant His-tagged isozyme OPR3 from Escherichia coli by nickel affinity chromatography	Solanum lycopersicum	Q9FEW9, Q9FEX0, Q9XG54	656998

Cloned/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
c-DNA library, gene OPR3 identified	Arabidopsis sp.	-	390778
-	Arabidopsis thaliana	-	390776
cloning, and DNA sequence determination and analysis, expression of gene At2g06050, encoding isozyme OPR3, in Escherichia coli as N-terminally fusion protein fused to S-tag-His6-maltose binding protein	Arabidopsis thaliana	-	657324
enzyme cloned from its cDNA, open reading frame encodes a 372 amino acids polypeptide, functionally expressed in Escherichia coli	Arabidopsis thaliana	Q8LAH7	390775
expression of the 3 isozymes OPR1-3 as N-terminally GST- or His-tagged enzymes in Escherichia coli	Arabidopsis thaliana	-	656998
OPR DNA and amino acid sequence determination and analysis, comparative genomic analysis of OPR genes, phylogenetic analysis and sequence comparisons, detailed overview	Arabidopsis thaliana	Q9FUP0	711596
OPR1 and OPR2, two closely related genes encodes 12-oxophytodienoic acid-10,11-reductases	Arabidopsis thaliana	-	390778
OPR3 expressed in functional form in Escherichia coli, cloning of OPR2 cDNA by reverse transcriptase-polymerase chain reaction, OPR2 expressed in insect cell cultures of Spodoptera frugiperda with transgenic Autographa californica baculoviruses and overexpressed in transgenic Arabidopsis thaliana	Arabidopsis thaliana	-	390779
recombinant expression in Escherichia coli, RNA interference is used to investigate the effect of knockdown expression of OPR1; recombinant expression in Escherichia coli, RNA interference is used to investigate the effect of knockdown expression of OPR2; recombinant expression of OPR3 in Escherichia coli	Arabidopsis thaliana	Q8GYB8, Q8LAH7, Q9FUP0	700835
subcloning of the OPR genes from a lambdaGEM11 genomic library	Arabidopsis thaliana	-	390777
OPR DNA and amino acid sequence determination and analysis, comparative genomic analysis of OPR genes, phylogenetic analysis and sequence comparisons, detailed overview	Chlamydomonas reinhardtii, Medicago truncatula	-	711596
construction of a cDNA library, phylogenetic analysis, DNA and amino acid sequence determination and analysis, expression as His-tagged enzyme in Escherichia coli strain BL21(DE3)	Oryza sativa	Q8H9F1	657134
expressed in Arabidopsis thaliana through transformation with Agrobacterium tumefaciens strain EHA105	Oryza sativa	-	689685
generating of a jasmonic acid-deficient rice line with suppressed expression of the gene encoding OPR3 with resistance to Magnaporthe grisea by introduction into rice plants by Agrobacterium-mediated transformation, OPR-interfering RNA plants show normal resistance to both an incompatible and a compatible race of Magnaporthe grisea; generating of a jasmonic acid-deficient rice line with suppressed expression of the genes encoding OPR1 with resistance to Magnaporthe grisea by introduction into rice plants by Agrobacterium-mediated transformation, OPR-interfering RNA plants show normal resistance to both an incompatible and a compatible race of Magnaporthe grisea	Oryza sativa	Q8H9F1	690761
OPR DNA and amino acid sequence determination and analysis, comparative genomic analysis of OPR genes, phylogenetic analysis and sequence comparisons, detailed overview	Oryza sativa, Physcomitrella patens, Picea sitchensis	-	711596
cDNA library construction, protein S64, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain BL21(DE3) as His-tagged protein	Pisum sativum	Q9AVK9	656976
construction of a genomic DNA library, identification and DNA sequence comparison of 6 genes of OPR belonging to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis, DNA sequence and gene structure determination and analysis, expression of isozymes in Escherichia coli strain BL21(DE3); construction of a genomic DNA library, identification of 6 genes of OPR belonging to the OPR enzyme family subgroup I, which is not required for jasmonate biosynthesis, DNA sequence and gene structure determination and analysis, expression of isozymes in Escherichia coli strain BL21(DE3)	Pisum sativum	Q76FR7, Q76FR8, Q76FR9, Q76FS0, Q76FS1, Q9AVK9	656802
OPR DNA and amino acid sequence determination and analysis, comparative genomic analysis of OPR genes, phylogenetic analysis and sequence comparisons, detailed overview	Populus trichocarpa	-	711596
expressed in Escherichia coli	Pseudomonas putida	-	678118
cloning and expression of a gene encoding old yellow enzyme	Saccharomyces carlsbergensis	-	390778
OPR DNA and amino acid sequence determination and analysis, comparative genomic analysis of OPR genes, phylogenetic analysis and sequence comparisons, detailed overview	Selaginella moellendorffii	-	711596
expression of His-tagged isozyme OPR1 in Escherichia coli, transient expression of isozyme OPR1 as enhanced-GFP- or RFP-fusion protein in onion epidermal cells for subcellular localization study; expression of His-tagged isozyme OPR2 in Escherichia coli; expression of His-tagged isozyme OPR3 in Escherichia coli, transient expression of isozyme OPR3 as enhanced-GFP- or RFP-fusion protein in onion epidermal cells for subcellular localization study	Solanum lycopersicum	Q9FEW9, Q9FEX0, Q9XG54	656998
OPR3 mutant enzymes are expressed in Escherichia coli	Solanum lycopersicum	-	699588
OPR DNA and amino acid sequence determination and analysis, comparative genomic analysis of OPR genes, phylogenetic analysis and sequence comparisons, detailed overview	Sorghum bicolor, Volvox carteri	-	711596
expressed in Arabidopsis thaliana through transformation with Agrobacterium tumefaciens strain GV3101	Zea mays	-	689661
OPR DNA and amino acid sequence determination and analysis, comparative genomic analysis of OPR genes, phylogenetic analysis and sequence comparisons, detailed overview	Zea mays	-	711596

EXPRESSION	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

ENGINEERING	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
E291L	Solanum lycopersicum	Q9FEW9	sixfold faster turnover than wild-type. Crystallization in same space group as wild-type, but with strikingly different cell constants. Appears as monomer in the crystal	676813
F74Y	Solanum lycopersicum	-	OPR3 mutant, change in substrate specificity, similar increase in stereoselectivity is observed for the mutant as compared to the wild-type enzyme	699588
F74Y/H244Y	Solanum lycopersicum	-	OPR3 double-mutant, reduction of (9S,13S)-12-oxo-phytodienoic acid is slower in the double-mutant as in the wild type enzyme and the single mutants, the protein crystallizes as a monomer with none of the dimer interactions retain	699588
H244Y	Solanum lycopersicum	-	OPR3 mutant, change in substrate specificity, similar increase in stereoselectivity is observed for the mutant as compared to the wild-type enzyme	699588
Y364F	Solanum lycopersicum	Q9FEW9	crystallization as a monomer with none of the dimer interactions retained	676813
additional information	Arabidopsis thaliana	-	construction of isozyme OPR3 mutant plants for subcellular localization study of the isozyme, overview	656998

Renatured/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
No entries in this field

APPLICATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
environmental protection	Arabidopsis thaliana	Q8GYB8, Q8LAH7, Q9FUP0	2,4,6-trinitrotoluene detoxofication, use of plants to remove environmental pollutants; 2,4,6-trinitrotoluene detoxofication, use of plants to remove environmental pollutants; 2,4,6-trinitrotoluene detoxofication, use of plants to remove environmental pollutants	700835

REF.	AUTHORS	TITLE	JOURNAL	VOL.	PAGES	YEAR	ORGANISM	LINK TO PUBMED	SOURCE
390772	Vick, B.A.; Zimmerman, D.C.	Levels of oxygenated fatty acids in young corn and sunflower plants	Plant Physiol.	69	1103-1108	1982	Helianthus annuus, Zea mays	PubMed
390773	Vick, B.A.; Zimmerman, D.C.	Biosynthesis of jasmonic acid by several plant species	Plant Physiol.	75	458-461	1984	Avena sativa, Helianthus annuus, Linum usitatissimum, Solanum melongena, Triticum aestivum, Zea mays	PubMed
390774	Vick, B.A.; Zimmerman, D.C.	Characterization of 12-oxo-phytodienoic acid reductase in corn	Plant Physiol.	80	202-205	1986	Zea mays	PubMed
390775	Schaller, F.; Weiler, E.W.	Molecular cloning and characterization of 12-oxophytodienoate reductase, an enzyme of the octadecanoid signaling pathway from Arabidopsis thaliana. Structural and functional relationship to yeast old yellow enzyme	J. Biol. Chem.	272	28066-28072	1997	Arabidopsis thaliana, Corydalis sempervirens, Saccharomyces carlsbergensis, Saccharomyces cerevisiae	PubMed
390776	Schaller, F.; Hennig, P.; Weiler, E.W.	12-Oxophytodienoate-10,11-reductase: occurrence of two isoenzymes of different specificity against stereoisomers of 12-oxophytodienoic acid	Plant Physiol.	118	1345-1351	1998	Arabidopsis sp., Arabidopsis thaliana, Bryonia dioica, Corydalis sempervirens, Saccharomyces cerevisiae, Zea mays	PubMed
390777	Biesgen, C.; Weiler, E.	Structure and regulation of OPR1 and OPR2, two closely related genes encoding 12-oxophytodienoic acid-10,11-reductases from Arabidopsis thaliana	Planta	208	155-165	1999	Arabidopsis thaliana, Bryonia dioica	PubMed
390778	Costa, C.L.; Arruda, P.; Benedetti, C.E.	An Arabidopsis gene induced by wounding functionally homologous to flavoprotein oxidoreductases	Plant Mol. Biol.	44	61-71	2000	Arabidopsis sp., Arabidopsis thaliana, Corydalis sempervirens, Saccharomyces carlsbergensis, Saccharomyces cerevisiae, Zea mays	PubMed
390779	Schaller, F.; Biesgen, C.; Muessig, C.; Altmann, T.; Weiler, E.W.	12-Oxophytodienoate reductase 3 (OPR3) is the isoenzyme involved in jasmonate biosynthesis	Planta	210	979-984	2000	Arabidopsis thaliana, Corydalis sempervirens, Saccharomyces cerevisiae	PubMed
390780	Breithaupt, C.; Strassner, J.; Breitinger, U.; Huber, R.; Macheroux, P.; Schaller, A.; Clausen, T.	X-ray structure of 12-oxophytodienoate reductase 1 provides structural insight into substrate binding and specificity within the family of OYE	Structure	9	419-429	2001	Solanum lycopersicum	PubMed
656802	Matsui, H.; Nakamura, G.; Ishiga, Y.; Toshima, H.; Inagaki, Y.; Toyoda, K.; Shiraishi, T.; Ichinose, Y.	Structure and expression of 12-oxophytodienoate reductase (subgroup I) genes in pea, and characterization of the oxidoreductase activities of their recombinant products	Mol. Genet. Genomics	271	1-10	2004	Pisum sativum	PubMed
656976	Ishiga, Y.; Funato, A.; Tachiki, T.; Toyoda, K.; Shiraishi, T.; Yamada, T.; Ichinose, Y.	Expression of the 12-oxophytodienoic acid 10,11-reductase gene in the compatible interaction between pea and fungal pathogen	Plant Cell Physiol.	43	1210-1220	2002	Pisum sativum	PubMed
656998	Strassner, J.; Schaller, F.; Frick, U.B.; Howe, G.A.; Weiler, E.W.; Amrhein, N.; Macheroux, P.; Schaller, A.	Characterization and cDNA-microarray expression analysis of 12-oxophytodienoate reductases reveals differential roles for octadecanoid biosynthesis in the local versus the systemic wound response	Plant J.	32	585-601	2002	Arabidopsis thaliana, Solanum lycopersicum	PubMed
657134	Sobajima, H.; Takeda, M.; Sugimori, M.; Kobashi, N.; Kiribuchi, K.; Cho, E.M.; Akimoto, C.; Yamaguchi, T.; Minami, E.; Shibuya, N.; Schaller, F.; Weiler, E.W.; Yoshihara, T.; Nishida, H.; Nojiri, H.; Omori, T.; Nishiyama, M.; Yamane, H.	Cloning and characterization of a jasmonic acid-responsive gene encoding 12-oxophytodienoic acid reductase in suspension-cultured rice cells	Planta	216	692-698	2003	Oryza sativa	PubMed
657324	Malone, T.E.; Madson, S.E.; Wrobel, R.L.; Jeon, W.B.; Rosenberg, N.S.; Johnson, K.A.; Bingman, C.A.; Smith, D.W.; Phillips, G.N., Jr.; Markley, J.L.; Fox, B.G.	X-ray structure of Arabidopsis At2g06050, 12-oxophytodienoate reductase isoform 3	Proteins	58	243-245	2004	Arabidopsis thaliana	-
676813	Breithaupt, C.; Kurzbauer, R.; Lilie, H.; Schaller, A.; Strassner, J.; Huber, R.; Macheroux, P.; Clausen, T.	Crystal structure of 12-oxophytodienoate reductase 3 from tomato: self-inhibition by dimerization	Proc. Natl. Acad. Sci. USA	103	14337-14342	2006	Solanum lycopersicum	PubMed
677040	Fox, B.G.; Malone, T.E.; Johnson, K.A.; Madson, S.E.; Aceti, D.; Bingman, C.A.; Blommel, P.G.; Buchan, B.; Burns, B.; Cao, J.; Cornilescu, C.; Doreleijers, J.; Ellefson, J.; Frederick, R.; Geetha, H.; Hruby, D.; Jeon, W.B.; Kimball, T.; Kunert, J.; Markley, J.L.; Newman, C.; Olson, A.; Peterson, F.C.; Phillips, G.N.; Primm, J.; Ramirez, B.; Rosenberg, N.S.; Runnels, M.; Seder, K.; Shaw, J.; Smith, D.W.; Sreenath, H.; Song, J.; Sussman, M.R.; Thao, S.; Troestler, D.; Tyler, E.; Tyler, R.; Ulrich, E.; Vinarov, D.; Vojtik, F.; Volkman, B.F.; Wesenberg, G.; Wrobel, R.L.; Zhang, J.; Zhao, Q.; Zolnai, Z.	X-ray structure of Arabidopsis At1g77680, 12-oxophytodienoate reductase isoform 1	Proteins	61	206-208	2005	Arabidopsis thaliana	PubMed
678118	Craig, D.H.; Moody, P.C.; Bruce, N.C.; Scrutton, N.S.	Reductive and oxidative half-reactions of morphinone reductase from Pseudomonas putida M10: a kinetic and thermodynamic analysis	Biochemistry	37	7598-7607	1998	Pseudomonas putida	PubMed
684429	Hall, M.; Stueckler, C.; Kroutil, W.; Macheroux, P.; Faber, K.	Asymmetric bioreduction of activated alkenes using cloned 12-oxophytodienoate reductase isoenzymes OPR-1 and OPR-3 from Lycopersicon esculentum (tomato): a striking change of stereoselectivity	Angew. Chem.	46	3934-3937	2007	Solanum lycopersicum	PubMed
689081	Engelberth, J.; Seidl-Adams, I.; Schultz, J.C.; Tumlinson, J.H.	Insect elicitors and exposure to green leafy volatiles differentially upregulate major octadecanoids and transcripts of 12-oxo phytodienoic acid reductases in Zea mays	Mol. Plant Microbe Interact.	20	707-716	2007	Zea mays	PubMed
689661	Gu, D.; Liu, X.; Wang, M.; Zheng, J.; Hou, W.; Wang, G.; Wang, J.	Overexpression of ZmOPR1 in Arabidopsis enhanced the tolerance to osmotic and salt stress during seed germination	Plant Sci.	174	124-130	2008	Zea mays	-
689685	Tani, T.; Sobajima, H.; Okada, K.; Chujo, T.; Arimura, S.; Tsutsumi, N.; Nishimura, M.; Seto, H.; Nojiri, H.; Yamane, H.	Identification of the OsOPR7 gene encoding 12-oxophytodienoate reductase involved in the biosynthesis of jasmonic acid in rice	Planta	227	517-526	2008	Oryza sativa	PubMed
690761	Yara, A.; Yaeno, T.; Hasegawa, M.; Seto, H.; Seo, S.; Kusumi, K.; Iba, K.	Resistance to Magnaporthe grisea in transgenic rice with suppressed expression of genes encoding allene oxide cyclase and phytodienoic acid reductase	Biochem. Biophys. Res. Commun.	376	460-465	2008	Oryza sativa	PubMed
699588	Breithaupt, C.; Kurzbauer, R.; Schaller, F.; Stintzi, A.; Schaller, A.; Huber, R.; Macheroux, P.; Clausen, T.	Structural basis of substrate specificity of plant 12-oxophytodienoate reductases	J. Mol. Biol.	392	1266-1277	2009	Solanum lycopersicum	PubMed
700835	Beynon, E.R.; Symons, Z.C.; Jackson, R.G.; Lorenz, A.; Rylott, E.L.; Bruce, N.C.	The role of oxophytodienoate reductases in the detoxification of the explosive 2,4,6-trinitrotoluene by Arabidopsis	Plant Physiol.	151	253-261	2009	Arabidopsis thaliana	PubMed
711596	Li, W.; Liu, B.; Yu, L.; Feng, D.; Wang, H.; Wang, J.	Phylogenetic analysis, structural evolution and functional divergence of the 12-oxo-phytodienoate acid reductase gene family in plants	BMC Evol. Biol.	9	90	2009	Arabidopsis thaliana, Chlamydomonas reinhardtii, Medicago truncatula, Oryza sativa, Physcomitrella patens, Picea sitchensis, Populus trichocarpa, Selaginella moellendorffii, Sorghum bicolor, Volvox carteri, Zea mays	PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 1.3.1.42)

NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM

IUBMB Enzyme Nomenclature

PROSITE Database of protein families and domains

SYSTERS

Protein Mutant Database

InterPro (database of protein families, domains and functional sites)

All organisms
Arabidopsis sp.
Arabidopsis thaliana
Avena sativa
Bryonia dioica
Chlamydomonas reinhardtii
Corydalis sempervirens
Helianthus annuus
Linum usitatissimum
Medicago truncatula
Oryza sativa
Physcomitrella patens
Picea sitchensis
Pisum sativum
Populus trichocarpa
Pseudomonas putida
Saccharomyces carlsbergensis
Saccharomyces cerevisiae
Selaginella moellendorffii
Solanum lycopersicum
Solanum melongena
Sorghum bicolor
Triticum aestivum
Volvox carteri
Zea mays