Information on EC 1.2.1.12 -

Information on EC 1.2.1.12 - glyceraldehyde-3-phosphate dehydrogenase (phosphorylating):

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EC NUMBER	COMMENTARY
1.2.1.12	-

RECOMMENDED NAME	GeneOntology No.
glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)	GO:0008943

REACTION	REACTION DIAGRAM	COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH + H+		bi-uni-uni-uni-ping-pong mechanism in which NAD+ and phosphate interact sequentially with the enzyme, followed in turn by the release of 3-phospho-D-glyceroyl phosphate, the addition of D-glyceraldehyde-3-phosphate, and the release of NADH. At pH 7.2, NAD+ binds to the enzyme in a rapid-equilibrium fashion, whereas at pH 8.8 there is rapid-equilibrium addition of both NAD+ and phosphate to the enzyme	Glycine max	-	287920
D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH + H+		B-specific oxidoreductase	Escherichia coli	P0A9B2	287927
D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH + H+		ordered ter bi mechanism characterized by the sequential addition of NAD+, glyceraldehyde 3-phosphate and phosphate to the enzyme and the sequential release of 3-phospho-D-glyceroyl phosphate and NADH from the enzyme	Homo sapiens	-	287945
D-glyceraldehyde 3-phosphate + phosphate + NAD+ = 3-phospho-D-glyceroyl phosphate + NADH + H+		-	-	-	-

REACTION TYPE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
oxidation	-	-	-	-
oxidation	Arabidopsis thaliana	-	-	663144, 673587, 676334
oxidation	Homo sapiens	-	-	671066, 671070, 673629
oxidation	Oryctolagus cuniculus	-	-	672402
oxidation	Spinacia oleracea	-	-	673587, 676334
oxidation	Kluyveromyces marxianus	-	-	674688
Phosphorylation	Solanum tuberosum	Q8LK04	-	675137
Phosphorylation	Rattus norvegicus	-	-	676108
Phosphorylation	Plasmodium falciparum	Q8IKK7	-	677046
redox reaction	-	-	-	-
reduction	-	-	-	-
reduction	Arabidopsis thaliana	-	-	663144, 673587, 676334
reduction	Plasmodium falciparum	-	-	671063
reduction	Homo sapiens	-	-	671066, 671070, 673629
reduction	Oryctolagus cuniculus	-	-	672402
reduction	Spinacia oleracea	-	-	673587, 676334
reduction	Kluyveromyces marxianus	-	-	674688

PATHWAY	KEGG Link	MetaCyc Link
Bifidobacterium shunt	-	P124-PWY
formaldehyde assimilation III (dihydroxyacetone cycle)	-	P185-PWY
gluconeogenesis I	-	GLUCONEO-PWY
glycerol degradation to butanol	-	PWY-7003
glycolysis I	-	GLYCOLYSIS
glycolysis II (from fructose-6P)	-	PWY-5484
glycolysis III (glucokinase)	-	ANAGLYCOLYSIS-PWY
glycolysis IV (plant cytosol)	-	PWY-1042
heterolactic fermentation	-	P122-PWY
sucrose biosynthesis	-	SUCSYN-PWY
xylose degradation IV	-	PWY-6901

SYSTEMATIC NAME	IUBMB Comments
D-glyceraldehyde-3-phosphate:NAD+ oxidoreductase (phosphorylating)	Also acts very slowly on D-glyceraldehyde and some other aldehydes; thiols can replace phosphate.

SYNONYMS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
3-phosphoglyceraldehyde dehydrogenase	-	-	-	-
A4-GAPDH	Arabidopsis thaliana	-	-	673587, 676334
A4-GAPDH	Spinacia oleracea	-	-	676334
A4-glyceraldehyde-3-phosphate dehydrogenase	Arabidopsis thaliana	-	-	673587
AB-GAPDH	Arabidopsis thaliana	-	-	676334
AnBn-GAPDH	Spinacia oleracea	-	-	673587
AsGAPDH	Ascaris suum	Q95YJ4	-	656745
BARS-38	-	-	-	-
CP 17/CP 18	-	-	-	-
cytosolic NAD-dependent glyceraldehyde 3-P dehydrogenase	Arabidopsis thaliana	P25858, Q56WJ4	-	689409
cytosolic NAD-dependent glyceraldehyde 3-P dehydrogenase	Oryctolagus cuniculus, Saccharomyces cerevisiae, Spinacia oleracea	-	-	689409
cytosolic phosphorylating glyceraldehyde-3-phosphate dehydrogenase	Solanum tuberosum	Q8LK04	-	675137
D-glyceraldehyde-3-phosphate dehydrogenase	Panulirus versicolor	P56649	-	654051
D-glyceraldehyde-3-phosphate dehydrogenase	Homo sapiens	-	-	671070, 684120, 684972, 687602
D-glyceraldehyde-3-phosphate dehydrogenase	Plasmodium falciparum	Q8IKK7	-	677046
D-glyceraldehyde-3-phosphate dehydrogenase	Bacillus megaterium	P23722	-	682151
D-glyceraldehyde-3-phosphate dehydrogenase	Mus musculus	-	-	685264, 688791
D-glyceraldehyde-3-phosphate dehydrogenase	Oryctolagus cuniculus	-	-	686305, 687602
D-glyceraldehyde-3-phosphate dehydrogenase	Rattus norvegicus	-	-	686305
D-glyceraldehyde-3-phosphate dehydrogenase	Thermotoga maritima	-	-	686481
D-glyceraldehyde-3-phosphate dehydrogenase	Leishmania mexicana	Q27890	-	687934
D-glyceraldehyde-3-phosphate: NAD+ oxidoreductase (phosphorylating)	Rattus norvegicus	-	-	688949
dehydrogenase, glyceraldehyde phosphate	-	-	-	-
dihydrogenase, glyceraldehyde phosphate	-	-	-	-
G3PD	-	-	-	-
G3PD	Homo sapiens	-	-	673629, 685025
G3PDH	Plasmodium brasilianum	B0F3R1	-	687069
G3PDH	Plasmodium coatneyi	B0F3R2	-	687069
G3PDH	Plasmodium cynomolgi	B0F3R8	-	687069
G3PDH	Plasmodium falciparum	B0F3R3	-	687069
G3PDH	Plasmodium fragile	B0F3R4	-	687069
G3PDH	Plasmodium knowlesi strain H	B0F3R5	-	687069
G3PDH	Plasmodium malariae	B0F3R6	-	687069
G3PDH	Plasmodium reichenowi	B0F3R7	-	687069
G3PDH	Plasmodium vivax	B0F3R9	-	687069
GADPH	Homo sapiens	-	-	697687
GAP1	Staphylococcus aureus	-	-	710757
GapA	Arabidopsis thaliana	-	A4 isoform of GAPDH	663144
GapA	Arabidopsis thaliana	-	-	676334
GapB	Arabidopsis thaliana	-	resulting from the fusion of GapA with the C-terminal half of the regulatory peptide CP12	676334
GAPC	Solanum tuberosum	Q8LK04	-	675137
GapC-1	Arabidopsis thaliana	-	-	700811
GapC1	Arabidopsis thaliana	P25858, Q56WJ4	isozyme	689409
GapC2	Arabidopsis thaliana	P25858, Q56WJ4	isozyme	689409
GAPD	Bos taurus, Canis lupus familiaris, Homo sapiens, Mus musculus, Oryctolagus cuniculus	-	somatic isoenzyme of glyceraldehyde-3-phosphate dehydrogenase	685292
GAPDH	-	-	-	-
GAPDH	Panulirus versicolor	P56649	-	654051
GAPDH	Oryctolagus cuniculus	-	-	654080, 654437, 670846, 672402, 673706, 685334, 685613, 686305, 686481, 686849, 687602, 689409, 713466
GAPDH	Trypanosoma cruzi	-	-	654711, 684403, 684427
GAPDH	Homo sapiens	-	-	654849, 654850, 654926, 671066, 671070, 672330, 673706, 676259, 684120, 684972, 685294, 686080, 686867, 687584, 687602, 688250, 688661, 712640
GAPDH	Pleurodeles waltl	Q8AXB5	-	655270
GAPDH	Xenopus laevis	-	-	655270
GAPDH	Rattus norvegicus	-	-	656072, 674503, 685334, 686305, 688949
GAPDH	Bos taurus	-	-	656594
GAPDH	Ascaris suum	Q95YJ4	-	656745
GAPDH	Arabidopsis thaliana	-	-	663144, 673587, 689409
GAPDH	Cavia porcellus	-	-	670846
GAPDH	Plasmodium falciparum	-	-	671063
GAPDH	Geobacillus stearothermophilus	-	-	672306
GAPDH	Spinacia oleracea	-	-	673587, 676334, 689409
GAPDH	Paracoccidioides brasiliensis	Q8X1X3	-	673986
GAPDH	Lactococcus lactis ssp. cremoris	-	-	674222
GAPDH	Streptococcus pyogenes	P0C0G6	-	674559
GAPDH	Kluyveromyces marxianus	-	-	674688
GAPDH	Mammalia	-	-	675023
GAPDH	Solanum tuberosum	Q8LK04	-	675137
GAPDH	Bacillus megaterium	-	-	679982, 682151
GAPDH	Sardina pilchardus	-	-	684121
GAPDH	Camelus dromedarius	-	-	684127
GAPDH	Mus musculus	-	-	685264, 685334, 688791
GAPDH	Entamoeba histolytica	-	-	686053, 686607, 687842
GAPDH	Thermotoga maritima	-	-	686481
GAPDH	Escherichia coli	-	-	687114
GAPDH	Lactobacillus plantarum	-	-	687344
GAPDH	Lactobacillus crispatus	-	-	687380
GAPDH	Leishmania mexicana	Q27890	-	687934
GAPDH	Neisseria meningitidis	-	-	688953
GAPDH	Saccharomyces cerevisiae	-	-	689409
GAPDH	Lentinus polychrous	D3K1B6	-	710790
GAPDH	Cryptosporidium parvum	-	-	711617
GAPDH1	-	-	-	-
GAPDH1	Staphylococcus aureus	-	-	712766
GAPDH2	-	-	-	-
GAPDS	Rattus norvegicus	-	-	676108
GAPDS	Bos taurus, Canis lupus familiaris, Homo sapiens, Mus musculus, Oryctolagus cuniculus	-	sperm-specific isoenzyme of glyceraldehyde-3-phosphate dehydrogenase	685292
GAPDS	Homo sapiens	O14556	sperm-specific GADPH	685294
GAPN	Neisseria meningitidis	-	-	688953
glyceraldehyde 3-phosphate dehydrogenase	Homo sapiens	-	-	686080
glyceraldehyde 3-phosphate dehydrogenase	Cryptosporidium parvum	-	-	711617
glyceraldehyde phosphate dehydrogenase (NAD)	-	-	-	-
glyceraldehyde-3 phosphate dehydrogenase	Entamoeba histolytica	Q27646	-	686053
glyceraldehyde-3-P-dehydrogenase	-	-	-	-
glyceraldehyde-3-phosphate dehydrogenase	Trypanosoma cruzi	-	-	654711
glyceraldehyde-3-phosphate dehydrogenase	Homo sapiens	-	-	654850, 671066, 672330, 673629, 673706, 676259
glyceraldehyde-3-phosphate dehydrogenase	Arabidopsis thaliana	-	-	663144, 673587, 676334
glyceraldehyde-3-phosphate dehydrogenase	Oryctolagus cuniculus	-	-	670846, 672402, 673706
glyceraldehyde-3-phosphate dehydrogenase	Cavia porcellus	-	-	670846
glyceraldehyde-3-phosphate dehydrogenase	Plasmodium falciparum	-	-	671063, 677046
glyceraldehyde-3-phosphate dehydrogenase	Geobacillus stearothermophilus	-	-	672306
glyceraldehyde-3-phosphate dehydrogenase	Spinacia oleracea	-	-	673587, 676334
glyceraldehyde-3-phosphate dehydrogenase	Paracoccidioides brasiliensis	Q8X1X3	-	673986
glyceraldehyde-3-phosphate dehydrogenase	Lactococcus lactis ssp. cremoris	-	-	674222
glyceraldehyde-3-phosphate dehydrogenase	Rattus norvegicus	-	-	674503, 676108
glyceraldehyde-3-phosphate dehydrogenase	Streptococcus pyogenes	P0C0G6	-	674559
glyceraldehyde-3-phosphate dehydrogenase	Kluyveromyces marxianus	-	-	674688
glyceraldehyde-3-phosphate dehydrogenase	Mammalia	-	-	675023
glyceraldehyde-3-phosphate dehydrogenase	Solanum tuberosum	Q8LK04	-	675137
glyceraldehyde-3-phosphate dehydrogenase (NAD)	-	-	-	-
glyceraldehyde-3-phosphate dehydrogenase 1	Staphylococcus aureus	-	-	710757
GPD	-	-	-	-
Gra3PDH	-	-	-	-
GraP-DH	-	-	-	-
HsGAPDH	Homo sapiens	-	-	671070
kmGAPDH1p	Kluyveromyces marxianus	-	formerly referred to as p37	674688
Larval antigen OVB95	-	-	-	-
Major larval surface antigen	-	-	-	-
NAD+-G-3-P dehydrogenase	-	-	-	-
NAD-dependent glyceraldehyde phosphate dehydrogenase	-	-	-	-
NAD-dependent glyceraldehyde-3-phosphate dehydrogenase	-	-	-	-
NAD-dependent non-phosphorylating glyceraldehyde-3-phosphate dehydrogenase	Neisseria meningitidis	-	-	688953
NAD-dependent phosphorylating glyceraldehyde-3-phosphate dehydrogenase	Neisseria meningitidis	-	-	688953
NAD-G3PDH	-	-	-	-
NADH-glyceraldehyde phosphate dehydrogenase	-	-	-	-
P-37	-	-	-	-
PfGAPDH	Plasmodium falciparum	-	-	671063, 677046
phosphoglyceraldehyde dehydrogenase	-	-	-	-
phosphorylating NAD+-dependent GAPDH	Tetrahymena pyriformis	-	-	688066
Plasmin receptor	-	-	-	-
Plasminogen-binding protein	-	-	-	-
pmGAPDH	Cavia porcellus	-	-	670846
rmGAPDH	Oryctolagus cuniculus	-	-	670846
TDH1	Saccharomyces cerevisiae	-	-	655286
TLAb	-	-	-	-
triose phosphate dehydrogenase	-	-	-	-

CAS REGISTRY NUMBER	COMMENTARY
9001-50-7	-

ORGANISM	COMMENTARY	LITERATURE	SEQUENCE CODE	SEQUENCE DB	SOURCE
Acipenser sp.	-	287964, 287967	-	-
Acipenser transmontanus	-	287942	-	-
Antheraea mylitta	-	695391	-	-
Apis mellifera	-	287971, 287976	-	-
Arabidopsis thaliana	-	656414, 663144, 673587, 676334, 700672, 700811	-	-
Arabidopsis thaliana	ecotype Columbia; isoform GapC1	689409	P25858	UniProt
Arabidopsis thaliana	isoform GapC2	689409	Q56WJ4	UniProt
Arabidopsis thaliana	isoform GAPCp1	700836	Q9SAJ6	UniProt
Arabidopsis thaliana	isoform GAPCp2	700836	Q84WR0	UniProt
Ascaris suum	-	656745	Q95YJ4	Uniprot
Bacillus cereus	-	287967, 287974	-	-
Bacillus coagulans	KU	287944, 287963	-	-
Bacillus coagulans KU	KU	287944	-	-
Bacillus megaterium	-	682151	P23722	SwissProt
Bacillus megaterium	strain DSM319	679982	P23722	SwissProt
Bacillus megaterium DSM319	strain DSM319	679982	P23722	SwissProt
Bacillus subtilis	-	287980	-	-
Bombus appositus	-	287971, 287976	-	-
Bombus nevadensis	-	287971, 287976	-	-
Bombus occidentalis	-	287971, 287976	-	-
Bos taurus	-	287905, 287911, 287967, 287979, 656594, 685292	-	-
Bradyrhizobium japonicum	CB1809	287902	-	-
Bradyrhizobium japonicum CB1809	CB1809	287902	-	-
Caenorhabditis elegans	-	287918, 287928	-	-
Camelus dromedarius	-	684127	-	-
Canis lupus familiaris	-	287967, 685292	-	-
Cavia porcellus	-	670846	-	-
Cryptosporidium parvum	-	697008, 711617	Q7YYQ9	UniProt
Cyanophora paradoxa	-	11542	-	-
Dissostichus mawsoni	-	287978	-	-
Drosophila melanogaster	-	287924	-	-
Dunaliella salina	-	697508	B1PL92	UniProt
Emericella nidulans	-	287915	-	-
Emericella nidulans	overproducing transformant and wild-type strain	287903	-	-
Entamoeba histolytica	-	687842	-	-
Entamoeba histolytica	fragment	686053	Q27646	UniProt
Entamoeba histolytica	fragment; strain HM1	686607	Q27646	UniProt
Entamoeba histolytica HM1	fragment; strain HM1	686607	Q27646	UniProt
Escherichia coli	-	287967, 287977, 287983, 698374, 699988	-	-
Escherichia coli	-	698925	P0A9B2	UniProt
Escherichia coli	enterohemorrhagic strain 86-24h11and enteropathogenic strain E2348/69	687114	-	-
Escherichia coli	wild-type and mutant enzymes N313T, Y317A, Y317G and N313T/Y317G	287927	P0A9B2	UniProt
Euglena gracilis	-	287973	-	-
Felis catus	-	287967	-	-
Gallus gallus	-	287967	-	-
Geobacillus stearothermophilus	-	287900, 287909, 287917, 287926, 287946, 287962, 287967, 287970, 654653, 672306	-	-
Geobacillus stearothermophilus	-	287985, 656119	P00362	Uniprot
Geobacillus stearothermophilus	wild-type and mutant W84F	287947	-	-
Geobacillus stearothermophilus	wild-type enzyme and mutant C149selenocysteine	287984	-	-
Glycine max	-	287902, 287920	-	-
Haloarcula vallismortis	-	287907	-	-
Hippoglossus sp.	halibut	287967	-	-
Homo sapiens	-	287900, 287910, 287913, 287925, 287932, 287945, 287950, 287967, 654849, 654850, 654926, 657393, 671070, 672330, 673629, 673706, 676259, 684120, 684972, 685025, 685292, 686080, 686867, 688250, 688661, 695298, 695602, 695939, 696174, 696824, 698989, 699035, 699592, 700133, 700503, 712640	-	-
Homo sapiens	-	671066, 685294, 687584, 687602, 697687, 698825	P04406	SwissProt
Homo sapiens	-	685294	O14556	UniProt
Huso huso	-	287942	-	-
Hypocrea koningii	under conditions of koningic acid production the koningic-acid-resistant isoenzyme GAPDH I is produced. In peptone-rich medium where non koningic acid is produced the koningic-acid-sensitive isoenzyme GAPDH II is produced in addition to GAPDH II	287908	-	-
Kluyveromyces marxianus	-	674688	-	-
Lactobacillus crispatus	strain ST1	687380	-	-
Lactobacillus plantarum	-	701175	-	-
Lactobacillus plantarum	strain LA 318	687344	-	-
Lactobacillus plantarum LA 318	-	701175	-	-
Lactococcus lactis	-	712937	-	-
Lactococcus lactis ssp. cremoris	MG1363 and relA acid-resistant mutant	674222	-	-
Leishmania mexicana	-	287986	-	-
Leishmania mexicana	-	687934	Q27890	Uniprot
Lentinus polychrous	commonly known as hed kradang	710790	D3K1B6	UniProt
Lentinus polychrous KPM3	commonly known as hed kradang	710790	D3K1B6	UniProt
Lobster	-	287967	-	-
Mammalia	-	675023	-	-
Marsilea quadrifolia	-	287956	O65843	Uniprot
Meleagris gallopavo	-	287967	-	-
Mesocricetus auratus	-	699035	-	-
Mus musculus	-	287957, 287958, 287982, 685264, 685292, 685334, 688791, 699686	-	-
Neisseria meningitidis	strain Z2491	688953	-	-
Neisseria meningitidis Z2491	strain Z2491	688953	-	-
Oplegnathus fasciatus	isoform GAPDH1	698019	B5AAJ5	UniProt
Oplegnathus fasciatus	isoform GAPDH2	698019	B5AAJ6	UniProt
Oryctolagus cuniculus	-	287900, 287904, 287926, 287933, 287934, 287946, 287953, 287957, 287959, 287965, 287967, 287969, 287972, 287978, 287981, 288344, 3247, 654080, 654437, 670846, 672402, 673706, 685292, 685334, 685613, 686305, 686481, 686849, 687602, 689409, 695902, 696421, 698989, 713466	-	-
Palinurus vulgaris	-	287951	-	-
Panulirus versicolor	-	287987	-	-
Panulirus versicolor	-	654051	P56649	Uniprot
Paracoccidioides brasiliensis	-	673986	Q8X1X3	Uniprot
Phenylobacterium immobile E	strain E, ATCC 30005	688066	-	-
Pinus sylvestris	-	287922, 287956	-	-
Pisum sativum	-	287936, 287967	-	-
Plasmodium brasilianum	fragment; strain Peru II	687069	B0F3R1	UniProt
Plasmodium brasilianum Peru II	fragment; strain Peru II	687069	B0F3R1	UniProt
Plasmodium coatneyi	fragment	687069	B0F3R2	UniProt
Plasmodium cynomolgi	fragment; strain Berok	687069	B0F3R8	UniProt
Plasmodium cynomolgi Berok	fragment; strain Berok	687069	B0F3R8	UniProt
Plasmodium falciparum	-	671063	-	-
Plasmodium falciparum	-	677046	Q8IKK7	SwissProt
Plasmodium falciparum	fragment; strain FVO	687069	B0F3R3	UniProt
Plasmodium falciparum FVO	fragment; strain FVO	687069	B0F3R3	UniProt
Plasmodium fragile	fragment; strain Nilgiri	687069	B0F3R4	UniProt
Plasmodium fragile Nilgiri	fragment; strain Nilgiri	687069	B0F3R4	UniProt
Plasmodium knowlesi strain H	fragment	687069	B0F3R5	UniProt
Plasmodium malariae	fragment; strain Uganda I	687069	B0F3R6	UniProt
Plasmodium malariae Uganda I	fragment; strain Uganda I	687069	B0F3R6	UniProt
Plasmodium reichenowi	fragment; strain CDC1	687069	B0F3R7	UniProt
Plasmodium reichenowi CDC1	fragment; strain CDC1	687069	B0F3R7	UniProt
Plasmodium vivax	fragment; strain Salvador I	687069	B0F3R9	UniProt
Plasmodium vivax Salvador	fragment; strain Salvador I	687069	B0F3R9	UniProt
Pleurodeles waltl	-	655270	Q8AXB5	SwissProt
Pseudomonas aeruginosa	-	287919	-	-
Pseudomonas sp.	strain pi9, antarctic psychrophilic bacterium	698639	A7LHM7	UniProt
Pseudomonas sp. pi9	strain pi9, antarctic psychrophilic bacterium	698639	A7LHM7	UniProt
Psithyrus suckleyi	-	287971, 287976	-	-
Rattus norvegicus	-	287913, 287925, 287929, 287960, 287967, 656072, 657393, 685334, 686305, 688949	-	-
Rattus norvegicus	-	696464	P04797	Uniprot
Rattus norvegicus	-	698925	Q9ESV6	UniProt
Rattus norvegicus	a two-hybrid interaction assay is performed, using as bait the mouse Kir6.2 C terminus, for the screening a rat heart cDNA library is used	674503	-	-
Rattus norvegicus	diabetic rat	695595	-	-
Rattus norvegicus	Sprague-Dawley rat	676108	-	-
Saccharomyces cerevisiae	-	287899, 287931, 287937, 287943, 287952, 287954, 287967, 287975, 288344, 655286, 689409	-	-
Saccharomyces cerevisiae	high-affnity cAMP-binding protein I and II	287948	-	-
Sardina pilchardus	European pilchard	684121	-	-
Schistosoma mansoni	-	698033	-	-
Sinapis alba	-	287923	-	-
Solanum tuberosum	-	675137	Q8LK04	SwissProt
Spinacia oleracea	-	287935, 287961, 288344, 673587, 676334, 689409	-	-
Staphylococcus aureus	-	710757, 712766	Q6GIL8	UniProt
Staphylococcus aureus	strain MRSA252, isoform GapA1	695363	Q6GIL8	UniProt
Staphylococcus aureus MRSA252	strain MRSA252, isoform GapA1	695363	Q6GIL8	UniProt
Starmerella bombicola	-	699355	B3FPE3	UniProt
Streptococcus equinus	-	697507	-	-
Streptococcus oralis	-	698256	-	-
Streptococcus pneumoniae	-	655720	Q97NL1	SwissProt
Streptococcus pyogenes	-	674559	P0C0G6	Uniprot
Streptomyces arenae	T�469, contains pentalenolactone-insensitive glyceraldehyde-3-phosphate dehydrogenase under conditions of production of pentalenolactone. In complex medium no pentalenolactone production is observed and a pentalenolactone-sensitive D-glyceraldehyde 3-phosphate dehydrogenase, rather than the insensitive enzyme can be detected	287930	-	-
Sus scrofa	-	287914, 287934, 287966, 287967, 3246	-	-
Tetrahymena pyriformis	strain E, ATCC 30005	688066	-	-
Thermotoga maritima	-	287906, 686481	-	-
Thermus aquaticus	-	287946, 287967	-	-
Thermus thermophilus	-	287926, 287938	-	-
Thermus thermophilus	strain HB 8	287968	-	-
Thermus thermophilus HB 8	strain HB 8	287968	-	-
Trypanosoma brucei	-	287900, 287901	-	-
Trypanosoma cruzi	-	287912, 654711, 684403, 684427, 696735	-	-
Trypanosoma cruzi	-	696747	P22513	Uniprot
Vigna radiata var. radiata	-	287949	-	-
Xanthomonas campestris	GAPDH is constitutively expressed	700056	Q4UY27	UniProt
Xenopus laevis	-	655270	-	-
Zymomonas mobilis	-	287916, 3241	-	-

GENERAL INFORMATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
metabolism	Streptococcus equinus	-	GAPDH is not involved in the control of the glycolytic flux. A strain overproducing GAPDH shows a high NADH-to-NAD+ ratio, but no significant differences in growth rate when grown on glucose or lactose	697507
metabolism	Escherichia coli	-	replacement of Escherichia coli GapA glyceraldehyde 3-phosphate dehydrogenase by Clostridium acetobutylicum GapC glyceraldehyde 3-phosphate dehydrogenase, EC 1.2.1.9 results in significant reduction of flux through the pentose phosphate pathway. Recombinant strains display increased NADPH availability, and consistently higher productivity than parent strains	699988
physiological function	Dunaliella salina	B1PL92	reduction of transcription by 41% and 67% using RNAi leads to transformants with sluggish motility and less active than wild-type	697508
physiological function	Streptococcus oralis	-	interaction of GAPDH with Porphyromonas gingivalis major fimbrae plays an important role in Porphyromonas gingivalis colonization. Amino acid residues 166 to 183 of Streptococcus oralis GAPDH exhibit the strongest binding activity toward rFimA, and the synthetic peptide corresponding to amino acid residues 166 to 183 of GAPDH, peptide DNFGVVEGLMTTIHAYTG inhibits Streptococcus oralis-Porphyromonas gingivalis biofilm formation in a dose-dependent manner. The peptide inhibits interbacterial biofilm formation by several oral streptococci and Porphyromonas gingivalis strains with different types of FimA	698256
physiological function	Homo sapiens	-	GAPDH physically associates with DNA repair enzyme APE1. This interaction allows GAPDH to convert the oxidized species of APE1 to the reduced form, thereby reactivating its endonuclease activity to cleave abasic sites	698825
physiological function	Xanthomonas campestris	Q4UY27	a GAPDH-deficient mutant can still grow in medium with glucose or other sugars as the sole carbon source, and no phosphofructokinase activity is detectable.The mutant can not utilize pyruvate as sole carbon source, whereas the wild-type can. Inactivation of GAPDH results in impairment of bacterial growth and virulence in the host plant, and reduction of intracellular ATP and extracellularpolysaccharide	700056
physiological function	Homo sapiens	-	downregulation of GAPDH using siRNA reduces both macrophage colony stimulating factor CSF-1 mRNA and protein levels, through destabilizing CSF-1 mRNA. CSF-1 mRNA half-lives are decreased by 50% in the presence of GAPDH siRNA. GAPDH associates with a large AU-rich containing regionof CSF-1	700133
physiological function	Homo sapiens	-	GAPDH is a translational suppressor of angiotensin II type 1 receptor expression and mediates the effect of H2O2 on angiotensin II type 1 receptor mRNA	700503
physiological function	Arabidopsis thaliana	-	GAPDH controls generation of H2O2 by the proapoptotic family member Bax and heat shock, which in turn suppresses cell death in yeast and plant cells	700672
physiological function	Arabidopsis thaliana	-	gapc-1 null mutant line shows a delay in growth, morpholigical alterations in siliques, and low seed number. Embryo development is altered, showing abortions and empty embryonic sacs in basal and apical siliques, respectively. Mutant shows a decrease in ATP levels and reduced respiratory rate as well as a decrease in the expression and activity of aconitase and succinate dehydrogenase and reduced levels of pyruvate and several Krebs cycle intermediates, and increased reactive oxygen species levels	700811
physiological function	Arabidopsis thaliana	Q84WR0, Q9SAJ6	double mutants lacking both plastidial isoforms gapcp1 and gapcp2 display a drastic phenotype of arrested root development, dwarfism, and sterility. In spite of their low gene expression level, GAPCp down-regulation leads to altered gene expression and to drastic changes in the sugar and amino acid balance of the plant. GAPCps are important for the synthesis of serine in roots. Serine supplementation to the growth medium rescues root developmental arrest and restores normal levels of carbohydrates and sugar biosynthetic activities in gapcp double mutants; double mutants lacking both plastidial isoforms gapcp1 and gapcp2 display a drastic phenotype of arrested root development, dwarfism, and sterility. In spite of their low gene expression level, GAPCp down-regulation leads to altered gene expression and to drastic changes in the sugar and amino acid balance of the plant. GAPCps are important for the synthesis of serine in roots. Serine supplementation to the growth medium rescues root developmental arrest and restores normal levels of carbohydrates and sugar biosynthetic activities in gapcp double mutants	700836
physiological function	Lactobacillus plantarum	-	the adhesion mechanism of the lactobacilli is in part due to GAPDH binding to human ABO-type blood group antigens expressed on human colonic mucin. After periodate oxidation of colonic mucin, adhesion of Lactobacillus plantarum LA 318 bacterial cells significantly decreases compared to normal human colonic mucin. High binding is observed to A and B group antigens, while binding to H group antigen is lower. No interaction is observed between GAPDH and various monosaccharides. GAPDH binding to the B-trisaccharide biotinyl polymer probe [Gala1-3 (Fuca1-2) Gal-] is significantly higher as compared to B-disaccharide, Lewis D-trisaccharide, 3-fucosyl-N-acetylglucosamine and a-N-acetylneuraminic acid biotinyl polymer-probes	701175
physiological function	Lentinus polychrous	D3K1B6	the GAPDH gene product is a heat shock protein which might be involved in the developmental phase of the Lentinus polychrous	710790

SUBSTRATE	PRODUCT	REACTION DIAGRAM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY/ Substrate	LITERATURE/ Substrate	COMMENTARY/ Product	LITERATURE/ Product	Reversibility r=reversible ir=irreversible ?=not specified
3-phospho-D-glyceroyl phosphate + NADH	D-glyceraldehyde 3-phosphate + phosphate + NAD+		Homo sapiens	-	-	287932	-	-	?
3-phospho-D-glyceroyl phosphate + NADH	D-glyceraldehyde 3-phosphate + phosphate + NAD+		Homo sapiens	-	-	287900, 287913, 287932	-	-	r
3-phospho-D-glyceroyl phosphate + NADH	D-glyceraldehyde 3-phosphate + phosphate + NAD+		Sus scrofa	-	-	287934	-	-	r
3-phospho-D-glyceroyl phosphate + NADH	D-glyceraldehyde 3-phosphate + phosphate + NAD+		Saccharomyces cerevisiae	-	-	287899	-	287899	?
3-phospho-D-glyceroyl phosphate + NADH	D-glyceraldehyde 3-phosphate + phosphate + NAD+		Saccharomyces cerevisiae	-	-	287931	-	287931	r
3-phospho-D-glyceroyl phosphate + NADH	D-glyceraldehyde 3-phosphate + phosphate + NAD+		Saccharomyces cerevisiae	-	-	287937	-	-	r
3-phospho-D-glyceroyl phosphate + NADH	D-glyceraldehyde 3-phosphate + phosphate + NAD+		Bos taurus	-	-	287979	-	-	r
3-phospho-D-glyceroyl phosphate + NADH	D-glyceraldehyde 3-phosphate + phosphate + NAD+		Oryctolagus cuniculus	-	-	287900, 287933, 287934, 287946	-	-	r
3-phospho-D-glyceroyl phosphate + NADH	D-glyceraldehyde 3-phosphate + phosphate + NAD+		Euglena gracilis	-	-	287973	-	-	r
3-phospho-D-glyceroyl phosphate + NADH	D-glyceraldehyde 3-phosphate + phosphate + NAD+		Geobacillus stearothermophilus	-	-	287900, 287909, 287946	-	-	r
3-phospho-D-glyceroyl phosphate + NADH	D-glyceraldehyde 3-phosphate + phosphate + NAD+		Pisum sativum	-	-	287936	-	-	r
3-phospho-D-glyceroyl phosphate + NADH	D-glyceraldehyde 3-phosphate + phosphate + NAD+		Trypanosoma brucei	-	-	287900	-	-	r
3-phospho-D-glyceroyl phosphate + NADH	D-glyceraldehyde 3-phosphate + phosphate + NAD+		Thermotoga maritima	-	-	287906	-	-	r
3-phospho-D-glyceroyl phosphate + NADH	D-glyceraldehyde 3-phosphate + phosphate + NAD+		Streptomyces arenae	-	-	287930	-	-	r
3-phospho-D-glyceroyl phosphate + NADH	D-glyceraldehyde 3-phosphate + phosphate + NAD+		Emericella nidulans	-	enzyme plays a central role in glyconeogenesis	287915	-	-	?
3-phospho-D-glyceroyl phosphate + NADH + H+	D-glyceraldehyde 3-phosphate + phosphate + NAD+		Spinacia oleracea	-	-	673587, 676334	-	-	?
3-phospho-D-glyceroyl phosphate + NADH + H+	D-glyceraldehyde 3-phosphate + phosphate + NAD+		Arabidopsis thaliana	-	-	663144, 673587, 676334	-	-	?
acetaldehyde + phosphate + NAD+	acetyl phosphate + NADH		Neisseria meningitidis	-	-	688953	-	-	-
acetaldehyde + phosphate + NAD+	acetyl phosphate + NADH		Homo sapiens	-	enzyme form E6.6 shows 27% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E6.8 shows 9% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 6% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 0.4% of the activity with D-glyceraldehyde 3-phosphate	287913	-	-	?
acetaldehyde 3-phosphate + NAD+	? + NADH		Neisseria meningitidis	-	-	688953	-	-	?
arsenate + GSH + NAD+ + glyceraldehyde 3-phosphate	arsenite + ?		Homo sapiens, Rattus norvegicus	-	-	657393	-	-	?
butyraldehyde + phosphate + NAD+	butyryl phosphate + NADH		Homo sapiens	-	enzyme form E6.6 shows 10% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E6.8 shows 15% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 12% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 0.9% of the activity with D-glyceraldehyde 3-phosphate	287913	-	-	?
D-glyceraldehyde 3-phosphate + 3-acetylpyridine hypoxanthine nucleotide + phosphate	3-phospho-D-glyceroyl phosphate + ?		Dissostichus mawsoni	-	1.3% of the activity with NAD+	287978	-	-	?
D-glyceraldehyde 3-phosphate + 3-acetylpyridine hypoxanthine nucleotide + phosphate	3-phospho-D-glyceroyl phosphate + ?		Oryctolagus cuniculus	-	3.2% of activity with NAD+	287978	-	-	?
D-glyceraldehyde 3-phosphate + 3-acetylpyridine NAD+ + phosphate	3-phospho-D-glyceroyl phosphate + ?		Saccharomyces cerevisiae	-	-	287937	-	-	?
D-glyceraldehyde 3-phosphate + 3-acetylpyridine NAD+ + phosphate	3-phospho-D-glyceroyl phosphate + ?		Dissostichus mawsoni	-	2.8% of the activity with NAD+	287978	-	-	?
D-glyceraldehyde 3-phosphate + 3-acetylpyridine NAD+ + phosphate	3-phospho-D-glyceroyl phosphate + ?		Oryctolagus cuniculus	-	4.5% of activity with NAD+	287978	-	-	?
D-glyceraldehyde 3-phosphate + arsenate + NAD+	3-phospho-D-glyceroyl arsenate + NADH		Homo sapiens	-	-	287932, 287950	-	-	?
D-glyceraldehyde 3-phosphate + arsenate + NAD+	3-phospho-D-glyceroyl arsenate + NADH		Sus scrofa	-	-	287934	-	-	?
D-glyceraldehyde 3-phosphate + arsenate + NAD+	3-phospho-D-glyceroyl arsenate + NADH		Saccharomyces cerevisiae	-	-	287937	-	-	ir
D-glyceraldehyde 3-phosphate + arsenate + NAD+	3-phospho-D-glyceroyl arsenate + NADH		Bos taurus	-	-	287979	-	-	?
D-glyceraldehyde 3-phosphate + arsenate + NAD+	3-phospho-D-glyceroyl arsenate + NADH		Oryctolagus cuniculus	-	-	287934	-	-	-
D-glyceraldehyde 3-phosphate + arsenate + NAD+	3-phospho-D-glyceroyl arsenate + NADH		Oryctolagus cuniculus	-	-	287969	-	-	ir
D-glyceraldehyde 3-phosphate + arsenate + NAD+	3-phospho-D-glyceroyl arsenate + NADH		Geobacillus stearothermophilus	-	-	287970	-	-	ir
D-glyceraldehyde 3-phosphate + arsenate + NAD+	3-phospho-D-glyceroyl arsenate + NADH		Streptomyces arenae	-	-	287930	-	-	?
D-glyceraldehyde 3-phosphate + arsenate + NAD+	3-phospho-D-glyceroyl arsenate + NADH		Thermus thermophilus	-	the product arseno-3-phosphoglycerate is decomposed readily to 3-phosphoglycerate	287938	-	-	ir
D-glyceraldehyde 3-phosphate + C8-(4-(2,2,6,6-tetramethyl-piperidinyl-1-oxyl))-NAD+ + phosphate	3-phospho-D-glyceroyl phosphate + C8-(4-(2,2,6,6-tetramethyl-piperidinyl-1-oxyl))-NADH		Huso huso	-	-	287942	-	-	?
D-glyceraldehyde 3-phosphate + N6-(2-carboxyethyl)-NAD+ + phosphate	3-phospho-D-glyceroyl phosphate + N6-(2-carboxyethyl)-NADH		Thermus thermophilus, Oryctolagus cuniculus, Geobacillus stearothermophilus	-	-	287926	-	-	?
D-glyceraldehyde 3-phosphate + N6-(4-(2,2,6,6-tetramethyl-piperidinyl-1-oxyl))-NAD+ + phosphate	3-phospho-D-glyceroyl phosphate + N6-(4-(2,2,6,6-tetramethyl-piperidinyl-1-oxy))-NADH		Huso huso	-	-	287942	-	-	?
D-glyceraldehyde 3-phosphate + NAD+	D-3-phosphoglycerate + NADH		Neisseria meningitidis	-	-	688953	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Gallus gallus, Meleagris gallopavo	-	-	287967	-	287967	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Cyanophora paradoxa	-	-	11542	-	11542	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Vigna radiata var. radiata	-	-	287949	-	287949	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Drosophila melanogaster	-	-	287924	-	287924	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Thermus aquaticus	-	-	287946	-	287946	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Thermus aquaticus	-	-	287967	-	287967	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Bacillus subtilis	-	-	287980	-	287980	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Mus musculus	-	-	287957	-	287957	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Mus musculus	-	-	287958	-	287958	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Mus musculus	-	-	287982	-	287982	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Thermus thermophilus	-	-	287926	-	287926	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Thermus thermophilus	-	-	287938	-	287938	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Thermus thermophilus	-	-	287968	-	287968	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Escherichia coli	P0A9B2	-	287927	-	287927	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Escherichia coli	-	-	287967	-	287967	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Escherichia coli	-	-	287977	-	287977	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Escherichia coli	-	-	287983	-	287983	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Homo sapiens	-	-	287910	-	287910	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Homo sapiens	-	-	287925	-	287925	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Homo sapiens	-	-	287945	-	287945	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Homo sapiens	-	-	287950	-	287950	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Homo sapiens	-	-	287967	-	287967	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Homo sapiens	-	-	696174, 712640	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Homo sapiens	-	-	287900	-	287900	r
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Homo sapiens	-	-	287913	-	287913	r
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Homo sapiens	-	-	287932	-	287932	r
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Rattus norvegicus	-	-	287913	-	287913	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Rattus norvegicus	-	-	287925	-	287925	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Rattus norvegicus	-	-	287929	-	287929	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Rattus norvegicus	-	-	287960	-	287960	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Rattus norvegicus	-	-	287967	-	287967	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Sus scrofa	-	-	3246	-	3246	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Sus scrofa	-	-	287914	-	287914	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Sus scrofa	-	-	287966	-	287966	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Sus scrofa	-	-	287967	-	287967	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Sus scrofa	-	-	287934	-	287934	r
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Saccharomyces cerevisiae	-	-	287899	-	287899	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Saccharomyces cerevisiae	-	-	287943	-	287943	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Saccharomyces cerevisiae	-	-	287948	-	287948	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Saccharomyces cerevisiae	-	-	287952	-	287952	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Saccharomyces cerevisiae	-	-	287954	-	287954	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Saccharomyces cerevisiae	-	-	287967	-	287967	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Saccharomyces cerevisiae	-	-	287975	-	287975	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Saccharomyces cerevisiae	-	-	288344	-	288344	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Saccharomyces cerevisiae	-	-	287931	-	287931	r
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Saccharomyces cerevisiae	-	-	287937	-	287937	r
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Bos taurus	-	-	287905	-	287905	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Bos taurus	-	-	287911	-	287911	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Bos taurus	-	-	287967	-	287967	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Bos taurus	-	-	287979	-	287979	r
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Oryctolagus cuniculus	-	-	3247	-	3247	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Oryctolagus cuniculus	-	-	287926	-	287926	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Oryctolagus cuniculus	-	-	287953	-	287953	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Oryctolagus cuniculus	-	-	287957	-	287957	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Oryctolagus cuniculus	-	-	287959	-	287959	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Oryctolagus cuniculus	-	-	287965	-	287965	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Oryctolagus cuniculus	-	-	287967	-	287967	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Oryctolagus cuniculus	-	-	287969	-	287969	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Oryctolagus cuniculus	-	-	287972	-	287972	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Oryctolagus cuniculus	-	-	287978	-	287978	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Oryctolagus cuniculus	-	-	287981	-	287981	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Oryctolagus cuniculus	-	-	288344	-	288344	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Oryctolagus cuniculus	-	-	713466	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Oryctolagus cuniculus	-	-	287900	-	287900	r
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Oryctolagus cuniculus	-	-	287933	-	287933	r
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Oryctolagus cuniculus	-	-	287934	-	287934	r
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Oryctolagus cuniculus	-	-	287946	-	287946	r
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Euglena gracilis	-	-	287973	-	287973	r
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Geobacillus stearothermophilus	-	-	287917	-	287917	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Geobacillus stearothermophilus	-	-	287926	-	287926	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Geobacillus stearothermophilus	-	-	287947	-	287947	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Geobacillus stearothermophilus	-	-	287962	-	287962	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Geobacillus stearothermophilus	-	-	287967	-	287967	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Geobacillus stearothermophilus	-	-	287970	-	287970	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Geobacillus stearothermophilus	-	-	287984	-	287984	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Geobacillus stearothermophilus	P00362	-	287985	-	287985	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Geobacillus stearothermophilus	-	-	654653	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Geobacillus stearothermophilus	-	-	287900	-	287900	r
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Geobacillus stearothermophilus	-	-	287909	-	287909	r
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Geobacillus stearothermophilus	-	-	287946	-	287946	r
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Emericella nidulans	-	-	287903	-	287903	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Emericella nidulans	-	-	287915	-	287915	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Bradyrhizobium japonicum	-	-	287902	-	287902	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Spinacia oleracea	-	-	287935	-	287935	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Spinacia oleracea	-	-	287961	-	287961	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Spinacia oleracea	-	-	288344	-	288344	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Pisum sativum	-	-	287967	-	287967	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Pisum sativum	-	-	287936	-	287936	r
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Glycine max	-	-	287902	-	287902	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Glycine max	-	-	287920	-	287920	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Pseudomonas aeruginosa	-	-	287919	-	287919	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Trypanosoma brucei	-	-	287901	-	287901	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Trypanosoma brucei	-	-	287900	-	287900	r
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Haloarcula vallismortis	-	-	287907	-	287907	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Canis lupus familiaris, Bacillus cereus	-	-	287967	-	287967	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Bacillus cereus	-	-	287974	-	287974	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Xenopus laevis	-	-	655270	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Caenorhabditis elegans	-	-	287918	-	287918	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Caenorhabditis elegans	-	-	287928	-	287928	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Trypanosoma cruzi	-	-	287912	-	287912	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Trypanosoma cruzi	-	-	696735	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Trypanosoma cruzi	P22513	-	696747	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Felis catus	-	-	287967	-	287967	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Bacillus coagulans	-	-	287944	-	287944	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Bacillus coagulans	-	-	287963	-	287963	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Apis mellifera	-	-	287971	-	287971	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Apis mellifera	-	-	287976	-	287976	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Pinus sylvestris	-	-	287922	-	287922	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Pinus sylvestris	-	-	287956	-	287956	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Leishmania mexicana	-	-	287986	-	287986	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Zymomonas mobilis	-	-	3241	-	3241	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Zymomonas mobilis	-	-	287916	-	287916	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Lobster	-	-	287967	-	287967	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Thermotoga maritima	-	-	287906	-	287906	r
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Sinapis alba	-	-	287923	-	287923	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Hypocrea koningii	-	-	287908	-	287908	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Panulirus versicolor	-	-	287987	-	287987	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Streptomyces arenae	-	-	287930	-	287930	r
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Huso huso, Acipenser transmontanus	-	-	287942	-	287942	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Palinurus vulgaris	-	-	287951	-	287951	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Marsilea quadrifolia	O65843	-	287956	-	287956	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Acipenser sp.	-	-	287964	-	287964	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Acipenser sp., Hippoglossus sp.	-	-	287967	-	287967	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Bombus nevadensis	-	-	287971	-	287971	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Bombus nevadensis	-	-	287976	-	287976	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Bombus occidentalis	-	-	287971	-	287971	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Bombus occidentalis	-	-	287976	-	287976	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Bombus appositus	-	-	287971	-	287971	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Bombus appositus	-	-	287976	-	287976	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Psithyrus suckleyi	-	-	287971	-	287971	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Psithyrus suckleyi	-	-	287976	-	287976	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Dissostichus mawsoni	-	-	287978	-	287978	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Pleurodeles waltl	Q8AXB5	-	655270	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Cryptosporidium parvum	Q7YYQ9	-	697008	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Cryptosporidium parvum	-	-	711617	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Pseudomonas sp.	A7LHM7	-	698639	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Staphylococcus aureus	Q6GIL8	-	710757	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Staphylococcus aureus	-	-	712766	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Lentinus polychrous	D3K1B6	-	710790	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Oryctolagus cuniculus	-	hydride transfer is a major rate-limiting step	287904	-	287904	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Bacillus subtilis	-	glycolytic enzyme	287980	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Homo sapiens	-	glycolytic enzyme	287925	-	-	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Homo sapiens	-	glycolytic enzyme	287945	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Emericella nidulans	-	enzyme plays a central role in glycolysis	287915	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Escherichia coli	-	key enzyme of glycolysis	287983	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Haloarcula vallismortis	-	key enzyme of glycolysis	287907	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Huso huso, Acipenser transmontanus	-	key enzyme of glycolysis	287942	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Hypocrea koningii	-	GAPDH I is responsible for glycolysis when koningic acid is produced	287908	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Pinus sylvestris	-	enzyme is involved in cytosolic glycolysis	287922	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Homo sapiens	-	absolute specificity for NAD+	695298	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Lactobacillus plantarum	-	-	687344	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Mus musculus	-	-	685292, 685334, 688791	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Escherichia coli	-	-	687114	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Homo sapiens	-	-	671070, 673629, 684120, 684972, 685025, 685292, 686080, 688250, 688661	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Rattus norvegicus	-	-	676108, 685334, 686305, 688949	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Saccharomyces cerevisiae	-	-	689409	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Bos taurus	-	-	685292	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Oryctolagus cuniculus	-	-	672402, 685292, 685334, 685613, 686305, 686481, 687602, 689409	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Tetrahymena pyriformis	-	-	688066	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Spinacia oleracea	-	-	689409	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Entamoeba histolytica	-	-	687842	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Lactococcus lactis	-	-	712937	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Canis lupus familiaris	-	-	685292	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Neisseria meningitidis	-	-	688953	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Trypanosoma cruzi	-	-	684427	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Trypanosoma cruzi	-	-	684403	-	-	r
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Kluyveromyces marxianus	-	-	674688	-	-	r
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Plasmodium falciparum	-	-	671063	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Leishmania mexicana	Q27890	-	687934	-	-	r
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Camelus dromedarius	-	-	684127	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Thermotoga maritima	-	-	686481	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Lactobacillus crispatus	-	-	687380	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Plasmodium falciparum	Q8IKK7	-	677046	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Homo sapiens	O14556	-	685294	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Homo sapiens	-	-	687602	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Homo sapiens	P04406	-	671066	-	-	r
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Homo sapiens	-	-	687584	-	-	r
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Sardina pilchardus	-	-	684121	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Entamoeba histolytica	Q27646	-	686607	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Entamoeba histolytica	Q27646	-	686053	-	-	r
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Plasmodium falciparum	B0F3R3	-	687069	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Plasmodium reichenowi	B0F3R7	-	687069	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Plasmodium knowlesi strain H	B0F3R5	-	687069	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Plasmodium coatneyi	B0F3R2	-	687069	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Plasmodium cynomolgi	B0F3R8	-	687069	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Plasmodium vivax	B0F3R9	-	687069	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Plasmodium fragile	B0F3R4	-	687069	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Plasmodium brasilianum	B0F3R1	-	687069	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Plasmodium malariae	B0F3R6	-	687069	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Arabidopsis thaliana	P25858, Q56WJ4	-	689409	-	-	?
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Geobacillus stearothermophilus	-	activity assay	672306	-	-	?
D-glyceraldehyde 3-phosphate + poly(ethylene glycol)-bound NAD+ + phosphate	3-phospho-D-glyceroyl phosphate + poly(ethylene glycol)-bound NADH		Thermus thermophilus, Oryctolagus cuniculus, Geobacillus stearothermophilus	-	-	287926	-	-	?
D-glyceraldehyde 3-phosphate + thio-NAD+ + phosphate	3-phospho-D-glyceroyl phosphate + thio-NADH		Dissostichus mawsoni	-	7.2% of the activity with NAD+	287978	-	-	?
D-glyceraldehyde 3-phosphate + thio-NAD+ + phosphate	3-phospho-D-glyceroyl phosphate + thio-NADH		Pisum sativum	-	thionicotinamide adenine dinucleotide is not as effective as NAD+	287936	-	-	?
D-glyceraldehyde 3-phosphate + thio-NAD+ + phosphate	3-phospho-D-glyceroyl phosphate + thio-NADH		Oryctolagus cuniculus	-	9.3% of activity with NAD+	287978	-	-	?
DL-glyceraldehyde + phosphate + NAD+	D-glyceroyl phosphate + NADH		Homo sapiens	-	enzyme form E6.6 shows no activity, enzyme form E6.8 shows 2.5% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 30% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 3.0% of the activity with D-glyceraldehyde 3-phosphate	287913	-	-	?
erythrose 4-phosphate + phosphate + NAD+	? + NADH		Homo sapiens	-	enzyme form E6.6 shows no activity, enzyme form E6.8 shows 1.2% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 25% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 1.5% of the activity with D-glyceraldehyde 3-phosphate	287913	-	-	?
glucose + phosphate + NAD+	? + NADH		Homo sapiens	-	enzyme form E6.6 shows no activity, enzyme form E6.8 shows 0.6% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 6.0% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 0.8% of the activity with D-glyceraldehyde 3-phosphate	287913	-	-	?
p-nitrophenyl acetate + H2O	p-nitrophenol + acetate		Escherichia coli	-	-	287977	-	-	?
propionaldehyde + phosphate + NAD+	propionyl phosphate + NADH		Homo sapiens	-	enzyme form E6.6 shows 33% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E6.8 shows 12% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 10% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 0.8% of the activity with D-glyceraldehyde 3-phosphate	287913	-	-	?
valeraldehyde + phosphate + NAD+	pentanoyl phosphate + NADH		Homo sapiens	-	enzyme form E6.6 shows no activity, enzyme form E6.8 shows 19% of the activity with D-glyceraldehyde 3-phosphate, enzyme form E8.5 shows 18% of the actity with D-glyceraldehyde 3-phosphate, enzyme form E9.0 shows 0.9% of the activity with D-glyceraldehyde 3-phosphate	287913	-	-	?
glyceraldehyde + NAD+	? + NADH		Neisseria meningitidis	-	-	688953	-	-	?
additional information	?	-	Geobacillus stearothermophilus	-	mutant enzyme C149selenocysteine shows selenoperoxidase activity with tert-butyl hydroperoxide and 3-carboxy 4-nitrobenzenethiol. Wild-type enzyme has no peroxidase activity	287984	-	-	-
additional information	?	-	Geobacillus stearothermophilus	-	mutant enzyme L187A/P188S is catalytically active not only with NAD+, as the wild-type enzyme, but also with NADP+	287909	-	-	-
additional information	?	-	Bacillus subtilis	-	wild-type enzyme has no activity with NADP+, the mutant enzyme D32A/L187N shows catalytic efficiency with NADP+ higher than that with NAD+	287980	-	-	-
additional information	?	-	Neisseria meningitidis	-	no activity with NADP+	688953	-	-	-
additional information	?	-	Emericella nidulans	-	the overproducing transformant grows faster and more efficient than the wild-type strain	287903	-	-	-
additional information	?	-	Saccharomyces cerevisiae	-	the isoproteins TLAb-a and TLAb-2 change depending on the cell growth phase, the carbon source and sodium chloride shock	287899	-	-	-
additional information	?	-	Saccharomyces cerevisiae	-	high-affinity binding of cAMP to D-glyceraldehyde 3-phosphate dehydrogenase may significantly reduce intracellular cAMP	287948	-	-	-
additional information	?	-	Caenorhabditis elegans	-	activity of isoenzyme 2 increases during postembryonic development	287928	-	-	-
additional information	?	-	Homo sapiens	-	enzyme is regulated by ATP and by D-glyceraldehyde 3-phosphate	287945	-	-	-
additional information	?	-	Pseudomonas aeruginosa	-	importance of the enzyme in glucose catabolism. Activity is reduced severalfold after growth on gluconeogenic substrates such as citrate compared to growth on glucose	287919	-	-	-
additional information	?	-	Homo sapiens	-	classical glycolytic protein involved exclusively in cytosolic energy production	654849	-	-	-
additional information	?	-	Homo sapiens	-	hypoxia upregulates the glycolytic enzymes glyceraldehyde-3-phosphate dehydrogenase in endothelial cells through a 5'-hypoxic regulatory element. Cell-specific patterns of HIF-1alpha and HIF-2alpha expression lead to cell-specific gene upregulation during hypoxia	654850	-	-	-
additional information	?	-	Saccharomyces cerevisiae	-	NADH-reductive stress in Saccharomyces cerevisiae induces the expression of the minor isoform of glyceraldehyde-3-phosphate dehydrogenase, TDH1	655286	-	-	-
additional information	?	-	Oryctolagus cuniculus	-	oxidation of GAPDH could be the signal for binding with nucleic acids and for change of quarternary structure. Theses events could facilitate GAPDH functioning in DNA reparation and tRNA transportation during oxidative stress	654437	-	-	-
additional information	?	-	Rattus norvegicus	-	plays a role in microtubule dynamics in the early secretory pathway	656072	-	-	-
additional information	?	-	Streptococcus pneumoniae	Q97NL1	the concerted action of both alpha-enolase and GAPDH on the bacterial cell surface might result in an enhancement of the pathogen�s ability to degrade the extracellular matrix and to invade host tissues	655720	-	-	-
additional information	?	-	Bos taurus	-	the enzyme is a GABA(A) receptor kinase linking glycolysis to neuronal inhibition	656594	-	-	-
additional information	?	-	Oryctolagus cuniculus	-	oxidation of SH-groups of the active site of GAPDH enhances its binding with total transfer RNA or with total DNA. Both NAD+ and NADH inhibit GAPDH-RNA or GAPDH-DNA interaction	654437	-	-	-
additional information	?	-	Streptococcus pneumoniae	Q97NL1	the enzyme has a specific plasmin- and plasminogen-binding activity, high affinity for plasmin and significantly lower affinity for plasminogen	655720	-	-	-
additional information	?	-	Lactobacillus crispatus	-	binds plasminogen	687380	-	-	-
additional information	?	-	Mus musculus	-	GAPDH is a Ca2+-dependent substrate of phosphorylase kinase although the rate of phosphorylation is very slow	685264	-	-	-

NATURAL SUBSTRATES	NATURAL PRODUCTS	REACTION DIAGRAM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY SUBSTRATE	LITERATURE (Substrate)	COMMENTARY PRODUCT	LITERATURE (Product)
3-phospho-D-glyceroyl phosphate + NADH	D-glyceraldehyde 3-phosphate + phosphate + NAD+		Emericella nidulans	-	enzyme plays a central role in glyconeogenesis	287915	-	-
3-phospho-D-glyceroyl phosphate + NADH + H+	D-glyceraldehyde 3-phosphate + phosphate + NAD+		Spinacia oleracea	-	-	673587, 676334	-	-
3-phospho-D-glyceroyl phosphate + NADH + H+	D-glyceraldehyde 3-phosphate + phosphate + NAD+		Arabidopsis thaliana	-	-	663144, 673587, 676334	-	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Staphylococcus aureus	Q6GIL8	-	710757	-	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Bacillus subtilis	-	glycolytic enzyme	287980	-	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Homo sapiens	-	glycolytic enzyme	287925, 287945	-	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Emericella nidulans	-	enzyme plays a central role in glycolysis	287915	-	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Escherichia coli	-	key enzyme of glycolysis	287983	-	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Haloarcula vallismortis	-	key enzyme of glycolysis	287907	-	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Huso huso, Acipenser transmontanus	-	key enzyme of glycolysis	287942	-	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Hypocrea koningii	-	GAPDH I is responsible for glycolysis when koningic acid is produced	287908	-	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH		Pinus sylvestris	-	enzyme is involved in cytosolic glycolysis	287922	-	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Homo sapiens	-	-	671070, 673629	-	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Rattus norvegicus	-	-	676108	-	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Oryctolagus cuniculus	-	-	672402	-	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Kluyveromyces marxianus	-	-	674688	-	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Plasmodium falciparum	-	-	671063	-	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Plasmodium falciparum	Q8IKK7	-	677046	-	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Homo sapiens	P04406	-	671066	-	-
D-glyceraldehyde 3-phosphate + phosphate + NAD+	3-phospho-D-glyceroyl phosphate + NADH + H+		Geobacillus stearothermophilus	-	activity assay	672306	-	-
additional information	?	-	Emericella nidulans	-	the overproducing transformant grows faster and more efficient than the wild-type strain	287903	-	-
additional information	?	-	Saccharomyces cerevisiae	-	the isoproteins TLAb-a and TLAb-2 change depending on the cell growth phase, the carbon source and sodium chloride shock	287899	-	-
additional information	?	-	Saccharomyces cerevisiae	-	high-affinity binding of cAMP to D-glyceraldehyde 3-phosphate dehydrogenase may significantly reduce intracellular cAMP	287948	-	-
additional information	?	-	Caenorhabditis elegans	-	activity of isoenzyme 2 increases during postembryonic development	287928	-	-
additional information	?	-	Homo sapiens	-	enzyme is regulated by ATP and by D-glyceraldehyde 3-phosphate	287945	-	-
additional information	?	-	Pseudomonas aeruginosa	-	importance of the enzyme in glucose catabolism. Activity is reduced severalfold after growth on gluconeogenic substrates such as citrate compared to growth on glucose	287919	-	-
additional information	?	-	Homo sapiens	-	classical glycolytic protein involved exclusively in cytosolic energy production	654849	-	-
additional information	?	-	Homo sapiens	-	hypoxia upregulates the glycolytic enzymes glyceraldehyde-3-phosphate dehydrogenase in endothelial cells through a 5'-hypoxic regulatory element. Cell-specific patterns of HIF-1alpha and HIF-2alpha expression lead to cell-specific gene upregulation during hypoxia	654850	-	-
additional information	?	-	Saccharomyces cerevisiae	-	NADH-reductive stress in Saccharomyces cerevisiae induces the expression of the minor isoform of glyceraldehyde-3-phosphate dehydrogenase, TDH1	655286	-	-
additional information	?	-	Oryctolagus cuniculus	-	oxidation of GAPDH could be the signal for binding with nucleic acids and for change of quarternary structure. Theses events could facilitate GAPDH functioning in DNA reparation and tRNA transportation during oxidative stress	654437	-	-
additional information	?	-	Rattus norvegicus	-	plays a role in microtubule dynamics in the early secretory pathway	656072	-	-
additional information	?	-	Streptococcus pneumoniae	Q97NL1	the concerted action of both alpha-enolase and GAPDH on the bacterial cell surface might result in an enhancement of the pathogen�s ability to degrade the extracellular matrix and to invade host tissues	655720	-	-
additional information	?	-	Bos taurus	-	the enzyme is a GABA(A) receptor kinase linking glycolysis to neuronal inhibition	656594	-	-

COFACTOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
3-acetylpyridine hypoxanthine nucleotide	Dissostichus mawsoni	-	1.3% of the activity with NAD+	287978	2D-image
3-acetylpyridine hypoxanthine nucleotide	Oryctolagus cuniculus	-	3.2% of activity with NAD+	287978	2D-image
3-acetylpyridine-NAD+	Saccharomyces cerevisiae	-	-	287937	2D-image
3-acetylpyridine-NAD+	Dissostichus mawsoni	-	2.8% of the activity with NAD+	287978	2D-image
3-acetylpyridine-NAD+	Oryctolagus cuniculus	-	4.5% of activity with NAD+ as cofactor	287978	2D-image
C6-(4-(2,2,6,6-tetramethyl-piperidinyl-1-oxyl))-NAD+	Huso huso	-	can replace NAD+ as cofactor	287942	-
N6-(2-carboxyethyl)-NAD+	Geobacillus stearothermophilus, Oryctolagus cuniculus, Thermus thermophilus	-	can replace NAD+ as cofactor	287926	2D-image
NAD+	Cyanophora paradoxa	-	-	11542	2D-image
NAD+	Saccharomyces cerevisiae	-	-	287899, 287943, 287948, 287952, 287954, 287967, 287975, 288344, 689409	2D-image
NAD+	Trypanosoma brucei	-	-	287900, 287901	2D-image
NAD+	Oryctolagus cuniculus	-	-	287900, 287904, 287933, 287934, 287946, 287953, 287957, 287965, 287967, 287969, 287972, 287978, 287981, 288344, 3247, 654437, 685292, 685334, 685613, 686305, 686481, 687602, 689409, 713466	2D-image
NAD+	Geobacillus stearothermophilus	-	-	287900, 287909, 287917, 287962, 287967, 287984, 654653	2D-image
NAD+	Homo sapiens	-	-	287900, 287910, 287913, 287925, 287932, 287945, 287967, 684120, 684972, 685025, 685292, 686080, 687584, 687602, 688250, 688661, 712640	2D-image
NAD+	Bradyrhizobium japonicum, Glycine max	-	-	287902	2D-image
NAD+	Emericella nidulans	-	-	287903, 287915	2D-image
NAD+	Bos taurus	-	-	287905, 287911, 287967, 287979, 685292	2D-image
NAD+	Thermotoga maritima	-	-	287906, 686481	2D-image
NAD+	Haloarcula vallismortis	-	-	287907	2D-image
NAD+	Hypocrea koningii	-	-	287908	2D-image
NAD+	Trypanosoma cruzi	-	-	287912, 684427, 696735	2D-image
NAD+	Rattus norvegicus	-	-	287913, 287925, 287929, 287960, 287967, 685334, 686305, 688949	2D-image
NAD+	Sus scrofa	-	NAD+-induced protein isomerization is the rate-limiting process for the formation of a catalytically competent holoenzyme	287914	2D-image
NAD+	Zymomonas mobilis	-	-	287916, 3241	2D-image
NAD+	Caenorhabditis elegans	-	-	287918, 287928	2D-image
NAD+	Pseudomonas aeruginosa	-	-	287919	2D-image
NAD+	Glycine max	-	at pH 8.8 there is rapid-equilibrium addition of both NAD+ and phosphate to the enzyme	287920	2D-image
NAD+	Pinus sylvestris	-	-	287922, 287956	2D-image
NAD+	Sinapis alba	-	-	287923	2D-image
NAD+	Drosophila melanogaster	-	-	287924	2D-image
NAD+	Geobacillus stearothermophilus, Oryctolagus cuniculus, Thermus thermophilus	-	poly(ethylene glycol)-bound NAD+ can replace NAD+ as cofactor	287926	2D-image
NAD+	Escherichia coli	-	-	287927, 287967, 287983, 687114	2D-image
NAD+	Streptomyces arenae	-	-	287930	2D-image
NAD+	Saccharomyces cerevisiae	-	activity with D-glyceraldehyde 3-phosphate in presence of 0.0006-0.006 mM NAD+: activity of the monomeric form is negligible or absent, high activity of the tetramer. 0.02-0.06 mM NAD+: markedly higher catalytic activity of the monomer. 2 mM NAD+: specific activities of monomeric and tetrameric forms are similar and equal to 0.06 mM/min. Activity with 3-phospho-D-glyceroyl phosphate: activity of the monomer is considerably higher than that of the tetramer	287931	2D-image
NAD+	Sus scrofa	-	-	287934, 287967, 3246	2D-image
NAD+	Spinacia oleracea	-	-	287935, 288344, 689409	2D-image
NAD+	Pisum sativum	-	contains 0.5-0.7 mol of NAD+ per mol of subunit	287936	2D-image
NAD+	Saccharomyces cerevisiae	-	weak binding of NAD+ to the enzyme	287937	2D-image
NAD+	Thermus thermophilus	-	-	287938, 287968	2D-image
NAD+	Acipenser transmontanus, Huso huso	-	-	287942	2D-image
NAD+	Bacillus coagulans	-	-	287944	2D-image
NAD+	Thermus aquaticus	-	-	287946, 287967	2D-image
NAD+	Geobacillus stearothermophilus	-	enzyme contains 4 equivalents of firmly bound NAD+	287946, 287970	2D-image
NAD+	Geobacillus stearothermophilus	-	effects of NAD+ binding on the luminescence of Trp84 and Trp310. NAD+-induced conformational change is sequential and subtle rearrangement in the structure of unligated subunits might be responsible for the negative cooperative behavior of NAD+ binding	287947	2D-image
NAD+	Vigna radiata var. radiata	-	-	287949	2D-image
NAD+	Homo sapiens	-	contains 3.0-3.5 M of NAD+ bound per mol of tetramer	287950	2D-image
NAD+	Palinurus vulgaris	-	the binding of NAD+ to apoenzyme in solution at 25�C is anticooperative	287951	2D-image
NAD+	Marsilea quadrifolia	O65843	-	287956	2D-image
NAD+	Mus musculus	-	-	287957, 287958, 287982, 685292, 685334	2D-image
NAD+	Oryctolagus cuniculus	-	between three and four molecules of NAD+ bound per tetramer	287959	2D-image
NAD+	Spinacia oleracea	-	contains about 1.8 mol NAD+ per mol of enzyme	287961	2D-image
NAD+	Bacillus coagulans	-	enzyme contains 4 equivalents of firmly bound NAD+	287963	2D-image
NAD+	Acipenser sp.	-	negative cooperativity in nicotinamide adenine dinucleotide binding	287964	2D-image
NAD+	Sus scrofa	-	2-3 mol of tightly bound NAD+	287966	2D-image
NAD+	Bacillus cereus	-	-	287967, 287974	2D-image
NAD+	Canis lupus familiaris	-	-	287967, 685292	2D-image
NAD+	Acipenser sp., Felis catus, Gallus gallus, Hippoglossus sp., Lobster, Meleagris gallopavo, Pisum sativum	-	-	287967	2D-image
NAD+	Bombus appositus, Bombus nevadensis, Bombus occidentalis, Psithyrus suckleyi	-	-	287971, 287976	2D-image
NAD+	Apis mellifera	-	contains 4 mol of NAD+ per mol of enzyme	287971	2D-image
NAD+	Euglena gracilis	-	-	287973	2D-image
NAD+	Apis mellifera	-	-	287976	2D-image
NAD+	Escherichia coli	-	contains less than 0.3 mol of NAD+ per mol of native protein	287977	2D-image
NAD+	Dissostichus mawsoni	-	contains 4 mol of NAD+ per mol of enzyme	287978	2D-image
NAD+	Bacillus subtilis	-	-	287980	2D-image
NAD+	Geobacillus stearothermophilus	-	dimers generated from the tetrameric enzyme are inactive but exhibit cooperativity in NAD+ binding	287985	2D-image
NAD+	Leishmania mexicana	-	-	287986	2D-image
NAD+	Panulirus versicolor	-	-	287987	2D-image
NAD+	Oryctolagus cuniculus	-	the cofactor is bound to two subunits of the tetrameric enzyme, which is consistent with the negative cooperativity of NAD+ binding to this enzyme	654080	2D-image
NAD+	Pleurodeles waltl	Q8AXB5	-	655270	2D-image
NAD+	Xenopus laevis	-	-	655270	2D-image
NAD+	Sardina pilchardus	-	dependent	684121	2D-image
NAD+	Camelus dromedarius	-	dependent	684127	2D-image
NAD+	Trypanosoma cruzi	-	dependent	684403	2D-image
NAD+	Homo sapiens	O14556	;	685294	2D-image
NAD+	Entamoeba histolytica	-	-	686053, 686607, 687842	2D-image
NAD+	Plasmodium brasilianum	B0F3R1	-	687069	2D-image
NAD+	Plasmodium coatneyi	B0F3R2	-	687069	2D-image
NAD+	Plasmodium cynomolgi	B0F3R8	-	687069	2D-image
NAD+	Plasmodium falciparum	B0F3R3	-	687069	2D-image
NAD+	Plasmodium fragile	B0F3R4	-	687069	2D-image
NAD+	Plasmodium knowlesi strain H	B0F3R5	-	687069	2D-image
NAD+	Plasmodium malariae	B0F3R6	-	687069	2D-image
NAD+	Plasmodium reichenowi	B0F3R7	-	687069	2D-image
NAD+	Plasmodium vivax	B0F3R9	-	687069	2D-image
NAD+	Lactobacillus plantarum	-	-	687344	2D-image
NAD+	Lactobacillus crispatus	-	-	687380	2D-image
NAD+	Leishmania mexicana	Q27890	dependent	687934	2D-image
NAD+	Tetrahymena pyriformis	-	dependent	688066	2D-image
NAD+	Mus musculus	-	GAPDH binds four NAD+ molecules per subunit	688791	2D-image
NAD+	Neisseria meningitidis	-	; absolute specificity for NAD+	688953	2D-image
NAD+	Arabidopsis thaliana	P25858, Q56WJ4	-	689409	2D-image
NAD+	Homo sapiens	-	absolute specifity for NAD+	695298	2D-image
NAD+	Staphylococcus aureus	Q6GIL8	-	710757, 712766	2D-image
NAD+	Lentinus polychrous	D3K1B6	-	710790	2D-image
NAD+	Cryptosporidium parvum	-	-	711617	2D-image
NAD+	Lactococcus lactis	-	-	712937	2D-image
NADH	Saccharomyces cerevisiae	-	cofactor	287899, 287931, 287937	2D-image
NADH	Geobacillus stearothermophilus	-	cofactor	287900, 287909, 287946	2D-image
NADH	Homo sapiens	-	cofactor	287900, 287913, 287932	2D-image
NADH	Oryctolagus cuniculus	-	cofactor	287900, 287933, 287934, 287946	2D-image
NADH	Trypanosoma brucei	-	cofactor	287900	2D-image
NADH	Thermotoga maritima	-	cofactor	287906	2D-image
NADH	Streptomyces arenae	-	cofactor	287930	2D-image
NADH	Sus scrofa	-	cofactor	287934	2D-image
NADH	Pisum sativum	-	cofactor	287936	2D-image
NADH	Euglena gracilis	-	cofactor	287973	2D-image
NADH	Bos taurus	-	cofactor	287979	2D-image
NADH	Oryctolagus cuniculus	-	-	654437, 670846, 672402	2D-image
NADH	Arabidopsis thaliana	-	-	663144, 673587, 676334	2D-image
NADH	Cavia porcellus	-	-	670846	2D-image
NADH	Plasmodium falciparum	-	one molecule NAD+ is bound to each of the four subunits of the tetrameric enzyme	671063	2D-image
NADH	Homo sapiens	-	each subunit is bound to one NAD+	671066	2D-image
NADH	Homo sapiens	-	-	671070, 673629, 695298	2D-image
NADH	Geobacillus stearothermophilus	-	-	672306	2D-image
NADH	Spinacia oleracea	-	-	673587, 676334	2D-image
NADH	Lactococcus lactis ssp. cremoris	-	-	674222	2D-image
NADH	Kluyveromyces marxianus	-	-	674688	2D-image
NADH	Rattus norvegicus	-	-	676108	2D-image
NADH	Plasmodium falciparum	Q8IKK7	-	677046	2D-image
NADP+	Bacillus subtilis	-	wild-type enzyme has no activity with NADP+. The mutant enzyme D32A/L187N shows catalytic efficiency with NADP+ higher than that with NAD+. Activity of mutant enzyme D32A with NAD+ is equal to that of the wild-type enzyme. Activity of mutant L187N with NAD+ is higher than that of the wild-type enzyme. Mutant enzymes D32A and L187N also show activity with NADP+, 3-7% of the activity with NAD+	287980	2D-image
NADPH	Spinacia oleracea	-	-	673587	2D-image
Thio-NAD+	Pisum sativum	-	thionicotinamide adenine dinucleotide is not as effective as NAD+	287936	2D-image
Thio-NAD+	Dissostichus mawsoni	-	7.2% of the activity with NAD+	287978	2D-image
Thio-NAD+	Oryctolagus cuniculus	-	9.3% of activity with NAD+	287978	2D-image
Thio-NAD+	Panulirus versicolor	P56649	the coenzyme analogue binds in a non-productive manner, resulting in a disordered thionicotinamide ring and rearranged active-site residues, effective as substrate to replace NAD+	654051	2D-image
C8-(4-(2,2,6,6-tetramethyl-piperidinyl-1-oxyl))-NAD+	Huso huso	-	can replace NAD+ as cofactor	287942	-
additional information	Neisseria meningitidis	-	undetectable activity with NADP+	688953	-

METALS and IONS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
(NH4)2SO4	Thermus thermophilus	-	0.045 M, 10fold activation	287938, 287968
CsCl	Thermus thermophilus	-	activates	287938
CsCl	Thermus thermophilus	-	activates; maximal activation: 20fold	287968
K+	Haloarcula vallismortis	-	KCl, activity is enhanced by increasing concentrations of the salt	287907
K+	Thermus thermophilus	-	KCl, activates	287938
K+	Vigna radiata var. radiata	-	activates to a small extent below 0.1 M	287949
K+	Thermus thermophilus	-	maximal activation: 22fold	287968
LiCl	Haloarcula vallismortis	-	activity is partly enhanced	287907
LiCl	Thermus thermophilus	-	activates	287938
LiCl	Thermus thermophilus	-	maximal activation: 10fold	287968
Na2SO4	Thermus thermophilus	-	up to 5fold activation	287938, 287968
NaCl	Haloarcula vallismortis	-	activity is enhanced by increasing concentrations of the salt	287907
NaCl	Thermus thermophilus	-	up to 5fold activation	287938
NH4Cl	Haloarcula vallismortis	-	activity is enhanced by increasing concentrations of the salt	287907
NH4Cl	Thermus thermophilus	-	0.1 M, 25fold activation	287938, 287968
RbCl	Haloarcula vallismortis	-	activity is enhanced by increasing concentrations of the salt. Optimum activity in 3 M RbCl	287907
Zn	Homo sapiens	-	enzyme form E8.5 contains 0.64 gatoms of zinc per mol of enzyme, enzyme form E9.0 contain 2.76 gatom of zinc per mol of enzyme	287913

INHIBITORS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
(8E,11E)-C15:2-anacardic acid	Trypanosoma cruzi	P22513	-	696747	2D-image
(8E,11E,14E)-C15:3-anacardic acid	Trypanosoma cruzi	P22513	-	696747	2D-image
(E)-2-hydroxy-6-(pentadec-8-en-1-yl)benzoic acid	Trypanosoma cruzi	P22513	-	696747	2D-image
(E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide	Oryctolagus cuniculus	-	treatment with (E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide between 0.001 and 1 mM induces the oligomerization of GAPDH, dithiothreitol reduces (E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide-induced aggregation in a concentration-dependent manner	687602	2D-image
(NH4)2SO4	Haloarcula vallismortis	-	-	287907	2D-image
1-hydroxy-2-oxo-3,3-bis(2-aminoethyl)-1-triazene	Homo sapiens, Oryctolagus cuniculus	-	-	687602	2D-image
2'-deoxy-2'-[(quinolin-7-ylcarbonyl)amino]adenosine	Leishmania mexicana	Q27890	-	687934	2D-image
2,3-diphosphoglycerate	Glycine max	-	-	287920	2D-image
2,3-diphosphoglycerate	Homo sapiens	-	-	287945	2D-image
2-(6-amino-2-methyl-9H-purin-9-yl)-5-(hydroxymethyl)tetrahydrofuran-3,4-diol	Leishmania mexicana	Q27890	-	687934	2D-image
2-(dodec-1-en-1-yl)-6-hydroxybenzoic acid	Trypanosoma cruzi	-	inhibition is not reversed or prevented by addition of Triton X-100. Noncompetitive with respect to both substrate and cofactor	696735	-
2-(hydroxymethyl)-5-[6-[(2-methylphenyl)amino]-9H-purin-9-yl]tetrahydrofuran-3,4-diol	Leishmania mexicana	Q27890	-	687934	2D-image
2-(hydroxymethyl)-5-[6-[(3-methylbutyl)amino]-9H-purin-9-yl]tetrahydrofuran-3,4-diol	Leishmania mexicana	Q27890	-	687934	2D-image
2-(hydroxymethyl)-5-[6-[(3-methylphenyl)amino]-9H-purin-9-yl]tetrahydrofuran-3,4-diol	Leishmania mexicana	Q27890	-	687934	2D-image
2-methyl-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
2-pentadecyl-6-hydroxybenzoic acid	Trypanosoma cruzi	-	inhibition is not reversed or prevented by addition of Triton X-100. Noncompetitive with respect to both substrate and cofactor	696735	-
2-[6-amino-8-(pyrimidin-2-ylsulfanyl)-9H-purin-9-yl]-5-(hydroxymethyl)tetrahydrofuran-3,4-diol	Leishmania mexicana	Q27890	-	687934	2D-image
2-[9-(2-deoxy-2-[[(2,4-dimethoxyphenyl)carbonyl]amino]pentofuranosyl)-9H-purin-6-yl]-2,3-dihydroisoquinoline	Leishmania mexicana	Q27890	-	687934	2D-image
3',4',5',5,7-pentamethoxyflavone	Entamoeba histolytica	Q27646	-	686053	2D-image
3',4'-methylenedioxy-5,6,7-trimethoxyflavone	Entamoeba histolytica	Q27646	-	686053	2D-image
3',5'-AMP	Apis mellifera	-	-	287971	2D-image
3-(1,3-benzodioxol-5-yl)-2-oxo-2H-chromen-6-ylacetate	Entamoeba histolytica	Q27646	-	686053	2D-image
3-(1,3-benzodioxol-5-yl)-2-oxo-2H-chromen-7-ylacetate	Entamoeba histolytica	Q27646	-	686053	2D-image
3-(1,3-benzodioxol-5-yl)-2-oxo-2H-chromen-8-ylacetate	Entamoeba histolytica	Q27646	-	686053	2D-image
3-(1,3-benzodioxol-5-yl)-2H-chromen-2-one	Entamoeba histolytica	Q27646	-	686053	2D-image
3-(1,3-benzodioxol-5-yl)-6-hydroxy-2H-chromen-2-one	Entamoeba histolytica	Q27646	-	686053	2D-image
3-(1,3-benzodioxol-5-yl)-6-nitro-2H-chromen-2-one	Entamoeba histolytica	Q27646	-	686053	2D-image
3-(1,3-benzodioxol-5-yl)-6-[[(1E)-1H-indol-3-ylmethylene]amino]-2H-chromen-2-one	Entamoeba histolytica	Q27646	-	686053	2D-image
3-(1,3-benzodioxol-5-yl)-6-[[(1E)-1H-pyrrol-2-ylmethylene]amino]-2H-chromen-2-one	Entamoeba histolytica	Q27646	-	686053	2D-image
3-(1,3-benzodioxol-5-yl)-6-[[(1E)-2-furylmethylene]amino]-2H-chromen-2-one	Entamoeba histolytica	Q27646	-	686053	2D-image
3-(1,3-benzodioxol-5-yl)-6-[[(1E)-pyridin-2-ylmethylene]amino]-2H-chromen-2-one	Entamoeba histolytica	Q27646	-	686053	2D-image
3-(1,3-benzodioxol-5-yl)-6-[[(1E)-thien-2-ylmethylene]amino]-2H-chromen-2-one	Entamoeba histolytica	Q27646	-	686053	2D-image
3-(1,3-benzodioxol-5-yl)-7-hydroxy-2H-chromen-2-one	Entamoeba histolytica	Q27646	-	686053	2D-image
3-(1,3-benzodioxol-5-yl)-8-hydroxy-2H-chromen-2-one	Entamoeba histolytica	Q27646	-	686053	2D-image
3-(chloroacetyl)-pyridine adenine dinculeotide	Geobacillus stearothermophilus	-	-	287909	2D-image
3-morpholino-sydnonimine	Mus musculus	-	the NO-generating compound inactivates by induction of a covalent binding of NAD+ to the enzyme. The superoxide anion released by 3-morpholino-sydnonimine potentiates the inactivation	287982	2D-image
3-phospho-D-glyceroyl phosphate	Pisum sativum	-	-	287936	2D-image
5'-deoxy-5'-[(diphenylacetyl)amino]adenosine	Leishmania mexicana	Q27890	-	687934	2D-image
5'-deoxy-5'-[[(4'-ethylbiphenyl-4-yl)carbonyl]amino]adenosine	Leishmania mexicana	Q27890	-	687934	2D-image
6-amino-3-(1,3-benzodioxol-5-yl)-2H-chromen-2-one	Entamoeba histolytica	Q27646	-	686053	2D-image
6-phosphogluconate	Pseudomonas aeruginosa	-	-	287919	2D-image
9-(2-aminoethyl)-8-thiophen-2-yl-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(2,4-dichlorophenyl)carbonyl]amino]pentofuranosyl)-N-(3-hydroxynaphthalen-1-yl)-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(2,4-dichlorophenyl)carbonyl]amino]pentofuranosyl)-N-naphthalen-1-yl-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(2,4-dichlorophenyl)carbonyl]amino]pentofuranosyl)-N-naphthalen-2-yl-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(2,4-dihydroxyphenyl)carbonyl]amino]pentofuranosyl)-N-naphthalen-1-yl-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(2,4-dimethoxyphenyl)carbonyl]amino]pentofuranosyl)-N-(3-hydroxynaphthalen-1-yl)-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(2,4-dimethoxyphenyl)carbonyl]amino]pentofuranosyl)-N-naphthalen-1-yl-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(2,4-dimethoxyphenyl)carbonyl]amino]pentofuranosyl)-N-naphthalen-2-yl-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(2-hydroxy-3-methoxyphenyl)carbonyl]amino]pentofuranosyl)-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(3,4-dihydroxyphenyl)carbonyl]amino]pentofuranosyl)-N-(naphthalen-1-ylmethyl)-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(3,4-dimethoxyphenyl)carbonyl]amino]pentofuranosyl)-N-(naphthalen-1-ylmethyl)-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(3,5-dihydroxyphenyl)carbonyl]amino]pentofuranosyl)-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(3,5-dimethoxyphenyl)carbonyl]amino]pentofuranosyl)-N-(2-methylphenyl)-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(3,5-dimethoxyphenyl)carbonyl]amino]pentofuranosyl)-N-(3-methylphenyl)-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(3,5-dimethoxyphenyl)carbonyl]amino]pentofuranosyl)-N-phenyl-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(3,5-dimethoxyphenyl)carbonyl]amino]pentofuranosyl)-N-[(3-methoxynaphthalen-1-yl)methyl]-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(3-ethoxyphenyl)carbonyl]amino]pentofuranosyl)-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(3-hydroxy-4-methoxyphenyl)carbonyl]amino]pentofuranosyl)-N-naphthalen-1-yl-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(3-hydroxyphenyl)carbonyl]amino]pentofuranosyl)-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(3-hydroxyphenyl)carbonyl]amino]pentofuranosyl)-N-(naphthalen-1-ylmethyl)-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(3-hydroxyphenyl)carbonyl]amino]pentofuranosyl)-N-naphthalen-1-yl-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(3-methoxyphenyl)carbonyl]amino]pentofuranosyl)-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(3-methoxyphenyl)carbonyl]amino]pentofuranosyl)-N-(2-methoxyphenyl)-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(3-methoxyphenyl)carbonyl]amino]pentofuranosyl)-N-phenyl-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(3-methoxyphenyl)carbonyl]amino]pentofuranosyl)-N-[(7-methylnaphthalen-1-yl)methyl]-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(4-heptylphenyl)carbonyl]amino]pentofuranosyl)-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(4-hydroxy-3-methoxyphenyl)carbonyl]amino]pentofuranosyl)-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(4-hydroxy-3-methoxyphenyl)carbonyl]amino]pentofuranosyl)-N-naphthalen-1-yl-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(4-methoxyphenyl)carbonyl]amino]pentofuranosyl)-N-(1-phenylethyl)-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(4-methoxyphenyl)carbonyl]amino]pentofuranosyl)-N-(2,5-dimethylphenyl)-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(4-methoxyphenyl)carbonyl]amino]pentofuranosyl)-N-(3,4-dimethylphenyl)-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-deoxy-2-[[(4-methoxyphenyl)carbonyl]amino]pentofuranosyl)-N-(3-hydroxynaphthalen-1-yl)-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-[[(4-chlorophenyl)carbonyl]amino]-2-deoxypentofuranosyl)-N-(3-hydroxynaphthalen-1-yl)-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-[[(4-chlorophenyl)carbonyl]amino]-2-deoxypentofuranosyl)-N-(naphthalen-1-ylmethyl)-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-(2-[[(4-chlorophenyl)carbonyl]amino]-2-deoxypentofuranosyl)-N-naphthalen-1-yl-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-[2-(benzylamino)ethyl]-8-thiophen-2-yl-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-[2-([[2,4-bis(acetyloxy)phenyl]carbonyl]amino)-2-deoxypentofuranosyl]-N-naphthalen-1-yl-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-[2-([[3,4-bis(acetyloxy)phenyl]carbonyl]amino)-2-deoxypentofuranosyl]-N-naphthalen-1-yl-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-[2-([[3-(acetyloxy)phenyl]carbonyl]amino)-2-deoxypentofuranosyl]-N-(naphthalen-1-ylmethyl)-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-[2-([[3-(acetyloxy)phenyl]carbonyl]amino)-2-deoxypentofuranosyl]-N-naphthalen-1-yl-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-[2-([[4-(acetyloxy)-3-methoxyphenyl]carbonyl]amino)-2-deoxypentofuranosyl]-N-(naphthalen-1-ylmethyl)-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-[2-deoxy-2-([[4-(dimethylamino)phenyl]carbonyl]amino)pentofuranosyl]-N-naphthalen-1-yl-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
9-[2-deoxy-2-[(phenylcarbonyl)amino]pentofuranosyl]-8-thiophen-2-yl-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
acetylleucine chloromethyl ketone	Homo sapiens	-	binds to GAPDH to modulate the conformation of the enzyme, the modified enzyme is susceptible to chymotrypsin-like protease activity, cleavage at TRp195-Arg196; irreversible inhibition, enzyme modified by acetylleucine chloromethyl ketone is deduced to be digested at the peptide bond Trp196-Arg196	654926	2D-image
ADP	Apis mellifera	-	-	287971	2D-image
ADP	Escherichia coli	-	inhibition of esterase activity with p-nitrophenyl acetate	287977	2D-image
ADP	Homo sapiens, Rattus norvegicus	-	moderately inhibits arsenate reductase activity	657393	2D-image
ADP-ribose	Panulirus versicolor	P56649	coenzyme analogue with a non-cooperative behaviour of binding, is a potent competitive inhibitor	654051	2D-image
Agaricic acid	Oryctolagus cuniculus	-	0.14 mM, 50% inhibition in absence of NAD+. 2 mM, 35% loss of activity in presence of 0.017 mM NAD+	287900	2D-image
Agaricic acid	Trypanosoma brucei	-	glyoxysomal enzyme: 0.2 mM, 60% loss of activity. Cytosolic enzyme: 2 mM, less than 20% loss of activity	287900	2D-image
alpha-chlorohydrin	Rattus norvegicus	Q9ESV6	the contraceptive activity of alpha-chlorohydrin and its apparent specificity for the sperm isoform in vivo are likely to be due to differences in metabolism to 3-chlorolactaldehyde in spermatozoa and somatic cells	698925	2D-image
AMP	Pseudomonas aeruginosa	-	-	287919	2D-image
AMP	Glycine max	-	-	287920	2D-image
AMP	Apis mellifera	-	-	287971	2D-image
AMP	Escherichia coli	-	inhibition of esterase activity with p-nitrophenyl acetate	287977	2D-image
AMP	Dissostichus mawsoni	-	18 mM, only 5% loss of activity	287978	2D-image
anti-TLAb serum	Saccharomyces cerevisiae	-	-	287899	-
ATP	Pseudomonas aeruginosa	-	-	287919	2D-image
ATP	Homo sapiens	-	noncompetitive inhibitor	287945	2D-image
ATP	Sus scrofa	-	at 0�C, loss of activity. Some of the lost activity is regained upon warming to room temperature	287966	2D-image
ATP	Apis mellifera	-	-	287971	2D-image
ATP	Euglena gracilis	-	10 mM, 50% inhibition	287973	2D-image
ATP	Escherichia coli	-	inhibition of esterase activity with p-nitrophenyl acetate	287977	2D-image
ATP	Dissostichus mawsoni	-	1 mM, 25% loss of activity	287978	2D-image
ATP	Homo sapiens, Rattus norvegicus	-	moderately inhibits arsenate reductase activity	657393	2D-image
ATP	Homo sapiens	-	inhibition is less pronounced for enzyme from sarcoma tissue as compared to normal muscle tissue	696174	2D-image
beta-mercaptoethanol	Neisseria meningitidis	-	strong inhibition at 10 mM	688953	2D-image
cAMP	Euglena gracilis	-	-	287973	2D-image
Cd2+	Camelus dromedarius	-	strong inhibition	684127	2D-image
CdCl2	Thermus thermophilus	-	-	287938, 287968	2D-image
CdCl2	Pleurodeles waltl	Q8AXB5	20 mM, about 30% inhibition. 10 mM, 70% inhibition	655270	2D-image
CdCl2	Xenopus laevis	-	20 mM, about 65% inhibition	655270	2D-image
CGP-3466	Homo sapiens	-	deprenyl-related compound that inhibits the pro-apoptotic activity of GAPDH	671070	2D-image
chalepin	Entamoeba histolytica	Q27646	-	686053	2D-image
chalepin	Entamoeba histolytica	-	natural inhibitor of GAPDH	687842	2D-image
Cu2+	Haloarcula vallismortis	-	1 mM CuCl2, complete inhibition	287907	2D-image
Cu2+	Thermus thermophilus	-	-	287938, 287968	2D-image
Cu2+	Bacillus cereus	-	-	287974	2D-image
Cu2+	Camelus dromedarius	-	-	684127	2D-image
CuCl2	Arabidopsis thaliana	-	1 mM	673587	2D-image
CuSO4	Pleurodeles waltl	Q8AXB5	0.001 mM, 90% inhibition within 2 min	655270	2D-image
CuSO4	Xenopus laevis	-	0.001 mM, 70% inhibition in 2 min	655270	2D-image
D-Glyceraldehyde 3-phosphate	Pisum sativum	-	substrate inhibition	287936	2D-image
D-Glyceraldehyde 3-phosphate	Homo sapiens	-	substrate inhibition	287945	2D-image
D-Glyceraldehyde 3-phosphate	Dissostichus mawsoni	-	substrate inhibition	287978	2D-image
demethylasterriquinone B1	Homo sapiens	-	binding of demethylasterriquinone B1 toGAPDH could disrupt phosphatase acting upon phosphatidylinositol lipids and thereby potentiate insulin signaling via the phosphatidylinositol-3-kinase pathway	688250	2D-image
Diamide	Arabidopsis thaliana	-	1 mM	673587	2D-image
diepoxybutane	Homo sapiens	-	incubation of GAPDH with bis-electrophiles results in inhibition of its catalytic activity, but only at high concentrations of diepoxybutane	686080	2D-image
dithiothreitol	Arabidopsis thaliana	-	DTT	663144	2D-image
dithiothreitol	Neisseria meningitidis	-	strong inhibition at 2 mM	688953	2D-image
Fe2+	Oryctolagus cuniculus	-	in the absence of quercetin, GAPDH can be oxidized by ferrous ions due to the formation of reactive oxygen species according to the following series of reactions	686849	2D-image
ferriprotoporphorin IX	Plasmodium falciparum	Q8IKK7	-	677046	2D-image
ferriprotoporphyrin IX	Plasmodium falciparum	-	-	671063	2D-image
ferriprotoporphyrin IX	Homo sapiens	-	enzyme is partially inactivated through oxidation of critical thiols	673629	2D-image
FK506-binding protein 36	Homo sapiens	-	i.e. FKBP36. The interaction between FKBP36 and GAPDH directly inhibits the catalytic activity of GAPDH. FKBP36 expression causes a significant reduction of the GAPDH level and activity in COS-7 cells. GAPDH is depleted by FKBP36 expression, particularly in the cytosolic fraction	698989	-
FK506-binding protein 36	Oryctolagus cuniculus	-	i.e. FKBP36, forms complexes with glyceraldehyde-3-phosphate dehydrogenase and Hsp90. Both proteins bind independently to different sites of the FKBP36 tetratricopeptide repeat domain. The interaction between FKBP36 and GAPDH directly inhibits the catalytic activity of GAPDH	698989	-
fumarate	Oryctolagus cuniculus	-	approximately 20% of GAPDH activity is lost by incubation with 0.5 mM fumarate for 24 h, and 100% activity is lost in incubations with 500 mM fumarate at 24 h, NADH in the presence or absence of D-glyceraldehyde 3-phosphate significantly accelerates the inactivation of GAPDH by fumarate	686305	2D-image
fumarate	Rattus norvegicus	-	inactivation of GAPDH by fumarate in vitro correlates with formation of S-(2-succinyl)cysteine, in diabetic compared with control rats fumarate and S-(2-succinyl)cysteine concentration increase approximately 5fold, accompanied by an about 25% decrease in GAPDH specific activity	686305	2D-image
glutathione	Arabidopsis thaliana	-	5 mM oxidized glutathione, GSSG, formation of mixed disulfide between glutathione and A4-GAPDH results in the inhibition of enzyme activity	673587	2D-image
glutathione	Spinacia oleracea	-	formation of mixed disulfide between glutathione and GAPDH results in the inhibition of enzyme activity	673587	2D-image
glutathione	Arabidopsis thaliana	P25858, Q56WJ4	inactivation with 10 mM glutathione is reversible upon addition of 20 mM dithiothreitol	689409	2D-image
glutathione	Oryctolagus cuniculus, Saccharomyces cerevisiae, Spinacia oleracea	-	inactivation with 10 mM glutathione is reversible upon addition of 20 mM dithiothreitol	689409	2D-image
gossypol	Trypanosoma brucei	-	-	287900	2D-image
guajaverin	Trypanosoma cruzi	P22513	molecular docking studies. Guajaverin is stabilized by five hydrogen bonds with the amino acids Ser165, Thr226, Arg249, Ser134, and Glu336	696747	2D-image
H2O2	Arabidopsis thaliana	-	-	663144	2D-image
H2O2	Oryctolagus cuniculus	-	-	672402, 687602	2D-image
H2O2	Arabidopsis thaliana	-	1 mM	673587	2D-image
H2O2	Spinacia oleracea	-	-	673587	2D-image
H2O2	Homo sapiens	-	-	673629, 687602, 688661	2D-image
H2O2	Oryctolagus cuniculus	-	GAPDH is oxidized by H2O2 which is likely formed due to the spontaneous dismutation of the superoxide anion that is formed during the autooxidation of quercetin that can result in the oxidation of SH-groups of GAPDH	686849	2D-image
Hg2+	Camelus dromedarius	-	strong inhibition	684127	2D-image
HgCl2	Haloarcula vallismortis	-	1 mM, complete inhibition	287907	2D-image
HgCl2	Thermus thermophilus	-	-	287938, 287968	2D-image
hydrogen peroxide	Tetrahymena pyriformis	-	maximum inhibition is observed at concentrations of 0.2 mM	688066	2D-image
iodoacetamide	Haloarcula vallismortis	-	5 mM, complete inhibition	287907	2D-image
iodoacetamide	Thermus thermophilus	-	-	287938	2D-image
iodoacetamide	Thermus thermophilus	-	0.5 mM, 9% inhibition	287968	2D-image
iodoacetamide	Homo sapiens	-	-	686080	2D-image
iodoacetate	Thermus thermophilus	-	-	287938	2D-image
iodoacetate	Homo sapiens	-	-	287950	2D-image
iodoacetate	Thermus thermophilus	-	0.2 mM, 67% inhibition	287968	2D-image
iodoacetate	Apis mellifera	-	-	287971	2D-image
iodoacetate	Euglena gracilis	-	-	287973	2D-image
iodoacetate	Bacillus cereus	-	-	287974	2D-image
iodoacetate	Escherichia coli	-	-	287977	2D-image
iodoacetate	Lactococcus lactis	-	-	712937	2D-image
K+	Vigna radiata var. radiata	-	0.2-1.0 M	287949	2D-image
K+	Camelus dromedarius	-	-	684127	2D-image
Koningic acid	Hypocrea koningii	-	irreversible inhibition, GAPDH I : 50% inhibition by 1 mM, no effect at 0.1 mM. GAPDH II: 50% inhibition by 0.01 mM. Under conditions of koningic acid production the koningic-acid-resistant isoenzyme GAPDH I is produced. In peptone-rich medium where non koningic acid is produced the koningic-acid-sensitive isoenzyme GAPDH II is produced in addition to GAPDH 1	287908	2D-image
Koningic acid	Homo sapiens, Rattus norvegicus	-	inhibits arsenate reductase activity and activity with D-glyceraldehyde 3-phosphate, phosphate and NAD+	657393	2D-image
mangiferin	Trypanosoma cruzi	P22513	-	696747	2D-image
Mn2+	Camelus dromedarius	-	-	684127	2D-image
N-(1,2,3,4-tetrahydronaphthalen-1-yl)adenosine	Leishmania mexicana	Q27890	-	687934	2D-image
N-(2-[[2-(hydroxymethyl)phenyl]sulfanyl]phenyl)adenosine	Leishmania mexicana	Q27890	-	687934	2D-image
N-(3-acetylnaphthalen-1-yl)-9-(2-deoxy-2-[[(2,4-dichlorophenyl)carbonyl]amino]pentofuranosyl)-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
N-(3-acetylnaphthalen-1-yl)-9-(2-deoxy-2-[[(4-methoxyphenyl)carbonyl]amino]pentofuranosyl)-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
N-(4-acetylnaphthalen-1-yl)-9-(2-deoxy-2-[[(4-methoxyphenyl)carbonyl]amino]pentofuranosyl)-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
N-(4-acetylnaphthalen-1-yl)-9-[2-deoxy-2-([[4-(methylamino)phenyl]carbonyl]amino)pentofuranosyl]-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
N-(diphenylmethyl)adenosine	Leishmania mexicana	Q27890	-	687934	2D-image
N-(naphthalen-2-ylmethyl)-9-pentofuranosyl-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
N-acetylcysteine	Homo sapiens	-	5 mM N-acetylcysteine significantly reduces G3PD activation induced by both H2O2 and ferric protoporphyrin IX	685025	2D-image
N-benzyl-9-(2-deoxy-2-[[(4-methoxyphenyl)carbonyl]amino]pentofuranosyl)-9H-purin-6-amine	Leishmania mexicana	Q27890	-	687934	2D-image
N-cycloheptyladenosine	Leishmania mexicana	Q27890	-	687934	2D-image
N-ethylmaleimide	Oryctolagus cuniculus	-	1 mM completely inactivates the enzyme in 10 min	686305	2D-image
N-[2-(6-amino-8-bromo-9H-purin-9-yl)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-3-yl]benzamide	Leishmania mexicana	Q27890	-	687934	2D-image
N-[2-(6-amino-9H-purin-9-yl)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-3-yl]-1H-benzimidazole-5-carboxamide	Leishmania mexicana	Q27890	-	687934	2D-image
N-[2-(6-amino-9H-purin-9-yl)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-3-yl]-3,4,5-trihydroxybenzamide	Leishmania mexicana	Q27890	-	687934	2D-image
N-[2-(6-amino-9H-purin-9-yl)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-3-yl]benzamide	Leishmania mexicana	Q27890	-	687934	2D-image
N-[2-(6-amino-9H-purin-9-yl)-4-hydroxy-5-(hydroxymethyl)tetrahydrofuran-3-yl]thiophene-2-carboxamide	Leishmania mexicana	Q27890	-	687934	2D-image
N-[2-(6-amino-9H-purin-9-yl)cyclopentyl]-3-bromobenzamide	Leishmania mexicana	Q27890	-	687934	2D-image
N-[2-(6-amino-9H-purin-9-yl)ethyl]-4-methylbenzamide	Leishmania mexicana	Q27890	-	687934	2D-image
Na+	Vigna radiata var. radiata	-	120 mM almost complete inhibition. Cys, GSH and deproteinated crude extract protect against inhibition	287949	2D-image
Na2S4O6	Thermus thermophilus	-	-	287938	-
Na2S4O6	Thermus thermophilus	-	0.1 mM, complete inhibition	287968	-
Na2S4O6	Bacillus cereus	-	-	287974	-
NAD+	Geobacillus stearothermophilus	-	competitive against NADH	287909	2D-image
NAD+	Homo sapiens	-	competitive against NADH	287932	2D-image
NAD+	Pisum sativum	-	substrate inhibition	287936	2D-image
NAD+	Sus scrofa	-	-	287966	2D-image
NADH	Pseudomonas aeruginosa	-	-	287919	2D-image
NADH	Glycine max	-	competitive with respect to NAD+ and phosphate	287920	2D-image
NADH	Homo sapiens	-	competitive against NAD+	287932	2D-image
NADH	Pisum sativum	-	competitive with NAD+ and with D-glyceraldehyde 3-phosphate	287936	2D-image
NADH	Homo sapiens	-	-	287945	2D-image
NADH	Geobacillus stearothermophilus	-	-	287970	2D-image
NADH	Homo sapiens, Rattus norvegicus	-	strongly inhibits arsenate reductase activity	657393	2D-image
NADH	Lactococcus lactis	-	competitive	712937	2D-image
NADP+	Neisseria meningitidis	-	75% inhibition in the presence of 2 mM NADP+	688953	2D-image
NADPH	Neisseria meningitidis	-	-	688953	2D-image
NEM	Apis mellifera	-	-	287971	2D-image
NEM	Euglena gracilis	-	1 mM, complete inhibition	287973	2D-image
NEM	Bacillus cereus	-	-	287974	2D-image
NiCl2	Thermus thermophilus	-	-	287968	2D-image
nitrogen oxide	Tetrahymena pyriformis	-	-	688066	2D-image
NO	Mus musculus	-	inactivation by induction of a covalent binding of NAD+ to the enzyme	287982	2D-image
NO	Tetrahymena pyriformis	-	diminishes GAPDH specific activity by 10%-20%	688066	2D-image
NO3-	Homo sapiens	-	uncompetitive inhibitor with NAD+, dead end inhibitor	287945	2D-image
oxidized glutathione	Arabidopsis thaliana	P25858, Q56WJ4	inactivation, at least partially reversible upon addition of dithiothreitol. Both residues C155 and C159 are found glutathionylated; inactivation, at least partially reversible upon addition of dithiothreitol. Both residues C155 and C159 are found glutathionylated	689409	2D-image
oxidized glutathione	Oryctolagus cuniculus, Spinacia oleracea	-	inactivation, at least partially reversible upon addition of dithiothreitol	689409	2D-image
p-hydroxymercuribenzoate	Geobacillus stearothermophilus	-	-	287970	2D-image
PCMB	Haloarcula vallismortis	-	5 mM, complete inhibition	287907	2D-image
PCMB	Euglena gracilis	-	1 mM, complete inhibition, partially reversed by dithiothreitol; 1 mM, inhibition is partially reversed by dithiothreitol	287973	2D-image
PCMB	Bos taurus	-	-	287979	2D-image
Pentalenolactone	Oryctolagus cuniculus	-	irreversible	287900, 288344	2D-image
Pentalenolactone	Geobacillus stearothermophilus	-	reversible	287900	2D-image
Pentalenolactone	Trypanosoma brucei	-	most potent inhibitor; reversible	287900	2D-image
Pentalenolactone	Haloarcula vallismortis	-	insensitive to	287907	2D-image
Pentalenolactone	Streptomyces arenae	-	pentalenolactone-sensitive enzyme is strongly inhibited, pentalenolactone-insensitive enzyme is not inhibited	287930	2D-image
Pentalenolactone	Saccharomyces cerevisiae	-	-	288344	2D-image
Pentalenolactone	Spinacia oleracea	-	irreversible	288344	2D-image
phosphate	Pisum sativum	-	substrate inhibition	287936	2D-image
pyridoxal 5'-phosphate	Mus musculus	-	inactivation with pseudo-first-order kinetics	287957	2D-image
pyridoxal 5'-phosphate	Oryctolagus cuniculus	-	-	287957	2D-image
quercetin	Trypanosoma cruzi	P22513	molecular docking studies. Quercetin is stabilized by two hydrogen bonds with the amino acids Ala198 and Pro253	696747	2D-image
S-(2-succinyl)cysteine	Rattus norvegicus	-	chemical modification by S-(2-succinyl)cysteine causes irreversible inactivation of glyceraldehyde-3-phosphate dehydrogenase in vitro. In diabetic rats, succination of GAPDH is increased in muscle, and the extent of succination correlates strongly with the decrease in specific activity of the enzyme	695595	2D-image
S-nitrosoglutathione	Arabidopsis thaliana	P25858, Q56WJ4	inactivation, at least partially reversible upon addition of dithiothreitol. Both residues C155 and C159 are found nitrosylated; inactivation, at least partially reversible upon addition of dithiothreitol. Both residues C155 and C159 are found nitrosylated; inactivation with 0.5 mM S-nitrosoglutathione is reversible upon addition of 20 mM dithiothreitol	689409	2D-image
S-nitrosoglutathione	Oryctolagus cuniculus	-	inactivation, at least partially reversible upon addition of dithiothreitol; inactivation with 0.5 mM S-nitrosoglutathione is reversible upon addition of 20 mM dithiothreitol	689409	2D-image
S-nitrosoglutathione	Saccharomyces cerevisiae	-	inactivation with 0.5 mM S-nitrosoglutathione is reversible upon addition of 20 mM dithiothreitol	689409	2D-image
S-nitrosoglutathione	Spinacia oleracea	-	inactivation, at least partially reversible upon addition of dithiothreitol; inactivation with 0.5 mM S-nitrosoglutathione is reversible upon addition of 20 mM dithiothreitol	689409	2D-image
sodium nitroprusside	Oryctolagus cuniculus	-	inhibition in presence of NAD+ is due primarily to active-site nitrosylation, covalent binding of NAD+ through a NO-dependent thiol intermediate	287981	2D-image
sodium nitroprusside	Mus musculus	-	the NO-generating compound inactivates by induction of a covalent binding of NAD+ to the enzyme	287982	2D-image
sodium nitroprusside	Tetrahymena pyriformis	-	maximum inhibition is observed at concentrations of 0.2 mM	688066	2D-image
Sodium tetrathionate	Thermus thermophilus	-	-	287938	2D-image
Sodium tetrathionate	Bacillus cereus	-	-	287974	2D-image
suramin	Trypanosoma brucei	-	cyosolic enzyme: competitive with NAD+. Effect on Km-value and maximal veocity of glyoxysomal enzyme	287900	2D-image
tiliroside	Trypanosoma cruzi	P22513	molecular docking studies. Tiliroside is stabilized by four hydrogen bonds with the amino acids Cys166, Ser134, and Ser110	696747	2D-image
Trinitrobenzenesulfonic acid	Mus musculus	-	inactivation with pseudo-first-order kinetics. D-glyceraldehyde-3-phosphate, NAD+, NADH and 3-phospho-D-glyceroyl phosphate almost completely protect from inactivation	287957	2D-image
Trinitrobenzenesulfonic acid	Oryctolagus cuniculus	-	-	287957	2D-image
Zn2+	Camelus dromedarius	-	-	684127	2D-image
ZnSO4	Pleurodeles waltl	Q8AXB5	20 mM, 70% inhibition	655270	2D-image
ZnSO4	Xenopus laevis	-	20 mM, 50% inhibition	655270	2D-image
monoclonal antibody 8B7	Oryctolagus cuniculus	-	antibody is specific for glyceraldehyde 3-phosphate dehydrogenase. In lysates of Sf21 cells, the antibody inhibits protein translation, possibly due to inhibition of the binding of glyceraldehyde 3-phosphate dehydrogenase to mRNA and tRNA	695902	-
additional information	Geobacillus stearothermophilus	-	the produced type I antibody induces a time-dependent decrease in the activity by 80-90% of the active holoenzyme and 25% of the apoenzyme	672306	-
additional information	Homo sapiens	-	inhibited by binding to the cell membrane	673629	-
additional information	Lactococcus lactis ssp. cremoris	-	the NADH/NAD+ ratio is shown to modulate the in vivo activity; the wild type GADPH is strongly inhibited in vitro by decreased pH values	674222	-
additional information	Homo sapiens	-	stauroporine does not inhibit stimulation of G3PD activity	685025	-
additional information	Mus musculus, Oryctolagus cuniculus, Rattus norvegicus	-	not inhibited by thiorphan and lipopolysaccharide	685334	-
additional information	Homo sapiens	-	not inhibited by DMSO, dibromomethane and 1,2-dibromoethane	686080	-
additional information	Oryctolagus cuniculus	-	succinate has no effect on enzyme activity	686305	-
additional information	Tetrahymena pyriformis	-	H2O2 does not significantly alter GAPDH-specific activity levels in cell free extracts	688066	-
additional information	Neisseria meningitidis	-	NADH, glucose-1-phosphate, AMP, ADP, ATP, and fructose-6-phosphate do not affect kinetic properties of GAPN and no change in cofactor preference from NAD+ to NADP+ in the presence of these metabolic intermediates is detected	688953	-
additional information	Homo sapiens	-	anti-GAPDH immunoglobulin G in the cerebrospinal fluid of patients with multiple sclerosis inhibits GAPDH glycolytic activity (38% or 58% inhibition after incubation of GAPDH with 0.002 or 0.004 mg, respectively)	712640	-
additional information	Lactococcus lactis	-	no inhibition by D-glucose 6-phosphate, D-fructose 6-phosphate, D-fructose 1,6-bisphosphate, dihydroxyacetone phosphate, phosphoenolpyruvate, pyruvate	712937	-

ACTIVATING COMPOUND	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
2,3-Dimercaptopropanol	Bos taurus	-	poor activation	287979	2D-image
2-mercaptoethanol	Haloarcula vallismortis	-	maximal activation at 5 mM, dependent on sulfhydryl compound	287907	2D-image
2-mercaptoethanol	Bos taurus	-	10 mM, 2fold activation of the oxidation of D-glyceraldehyde 3-phosphate	287979	2D-image
acetone	Thermus thermophilus	-	activates	287938	2D-image
Cys	Bos taurus	-	10 mM, 2.8fold activation of the oxidation of D-glyceraldehyde 3-phosphate	287979	2D-image
dithioerythritol	Bos taurus	-	10 mM, 2fold activation of the oxidation of D-glyceraldehyde 3-phosphate	287979	2D-image
DMSO	Trypanosoma cruzi	-	activity is increased about 2fold in the presence of 5% DMSO (v/v)	684403	2D-image
Doxorubicin	Homo sapiens	-	treatment results in increased nuclear localization of expressed wild-type GAPDH, where it protects telomeres against rapid degradation, concomitant with increased resistance to the growth-inhibitory effects of the drug	699592	2D-image
EDTA	Bos taurus	-	poor activation	287979	2D-image
ethanol	Thermus thermophilus	-	30% v/v, 5fold activation	287938, 287968	2D-image
Ferric protoporphyrin IX	Homo sapiens	-	145% increase of activity at 0.04 mM ferric protoporphyrin IX after 30 min exposure to stauroporin	685025	2D-image
gemcitabine	Homo sapiens	-	treatment results in increased nuclear localization of expressed wild-type GAPDH, where it protects telomeres against rapid degradation, concomitant with increased resistance to the growth-inhibitory effects of the drug	699592	2D-image
GSH	Haloarcula vallismortis	-	dependent on sulfhydryl compound	287907	2D-image
GSH	Bos taurus	-	poor activation	287979	2D-image
H2O2	Homo sapiens	-	186% increase of activity at 1 mM H2O2	685025	2D-image
H2O2	Homo sapiens	-	subapoptotic doses lead to strong hyperactivation, related to mild oxidative stress. U937 cells hyperactivate glyceraldehyde 3-phosphate dehydrogenase with the same timing observed for glyceraldehyde 3-phosphate dehydrogenase alterations from apoptogenic doses of H2O2	695602	2D-image
NAD+	Entamoeba histolytica	Q27646	highest activity in the presence of 0.2 mM NAD+ at 30�C	686607	2D-image
NADPH	Pseudomonas aeruginosa	-	slight activation	287919	2D-image
propan-1-ol	Thermus thermophilus	-	activates	287968	2D-image
propan-2-ol	Thermus thermophilus	-	activates	287968	2D-image
tetrabutylammonium chloride	Thermus thermophilus	-	50% activation	287968	2D-image
Triton X-100	Homo sapiens	-	-	685025	2D-image
methanol	Trypanosoma cruzi	-	activity is increased about 6fold in the presence of 5% methanol (v/v)	684403	2D-image
additional information	Bacillus cereus	-	enzyme contains 8 SH groups per tetramer of which 4 are highly reactive and essential for activity	287974	-
additional information	Homo sapiens	-	human TATA-binding protein-associated factor II 68-TEC fusion protein-dependent transcription is enhanced by GAPDH	684972	-
additional information	Homo sapiens	-	GAPDH functions as a coactivator with high selectivity for androgen receptor and enhances androgen receptor transactivation independent of its glycolytic activity	687584	-
additional information	Homo sapiens	-	20% increase of GADPH expression 3 h post concentric exercise	697687	-

KM VALUE [mM]	KM VALUE [mM] Maximum	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
0.01	-	1,3-Diphosphoglyceric acid	Homo sapiens	-	enzyme form E6.6, pH 7	287913	2D-image
0.012	-	1,3-Diphosphoglyceric acid	Homo sapiens	-	enzyme form E8.5, pH 7	287913	2D-image
0.018	-	1,3-Diphosphoglyceric acid	Homo sapiens	-	enzyme form E9.0, pH 7	287913	2D-image
0.158	-	3-acetyl-NAD+	Oryctolagus cuniculus	-	-	287978	2D-image
0.62	-	3-acetyl-NAD+	Dissostichus mawsoni	-	-	287978	2D-image
1.43	-	3-acetylpyridine hypoxanthine nucleotide	Oryctolagus cuniculus	-	-	287978	2D-image
8.15	-	3-acetylpyridine hypoxanthine nucleotide	Dissostichus mawsoni	-	-	287978	2D-image
0.002	-	3-phospho-D-glyceroyl phosphate	Homo sapiens	-	enzyme form E6.8, pH 7	287913	2D-image
0.032	-	3-phospho-D-glyceroyl phosphate	Homo sapiens	-	enzyme form E9.0, pH 9	287913	2D-image
0.034	-	3-phospho-D-glyceroyl phosphate	Geobacillus stearothermophilus	-	-	287900	2D-image
0.035	-	3-phospho-D-glyceroyl phosphate	Homo sapiens	-	enzyme form E8.5, pH 9	287913	2D-image
0.042	-	3-phospho-D-glyceroyl phosphate	Homo sapiens	-	enzyme form E6.6, pH 9	287913	2D-image
0.1	-	3-phospho-D-glyceroyl phosphate	Trypanosoma brucei	-	glyoxysomal enzyme	287900	2D-image
0.13	-	3-phospho-D-glyceroyl phosphate	Oryctolagus cuniculus	-	-	287900	2D-image
0.14	-	3-phospho-D-glyceroyl phosphate	Homo sapiens	-	-	287900	2D-image
0.14	-	3-phospho-D-glyceroyl phosphate	Trypanosoma brucei	-	cytosolic enzyme	287900	2D-image
0.172	-	3-phospho-D-glyceroyl phosphate	Homo sapiens	-	enzyme form E6.8, pH 9	287913	2D-image
0.47	-	3-phospho-D-glyceroyl phosphate	Euglena gracilis	-	-	287973	2D-image
0.31	-	acetaldehyde	Neisseria meningitidis	-	recombinant enzyme, in 50 mM Tricine buffer (pH 8.5), at 25�C	688953	2D-image
1	-	arsenate	Bacillus cereus	-	-	287974	2D-image
1.54	-	arsenate	Bos taurus	-	pH 8.9	287979	2D-image
1.7	-	arsenate	Bos taurus	-	pH 8.0	287979	2D-image
0.2	-	C6-(4-(2,2,6,6-tetramethyl-piperidinyl-1-oxyl))-NAD+	Huso huso	-	-	287942	-
0.061	-	C8-(4-(2,2,6,6-tetramethyl-piperidinyl-1-oxyl))-NAD+	Huso huso	-	-	287942	-
0.0102	-	D-glyceraldehyde	Trypanosoma cruzi	-	in the presence of 5% (v/v) methanol, in TEA-HCl buffer (pH 7.5) at 25�C	684403	2D-image
0.0108	-	D-glyceraldehyde	Trypanosoma cruzi	-	in the presence of 5% (v/v) DMSO, in TEA-HCl buffer (pH 7.5) at 25�C	684403	2D-image
0.0393	-	D-glyceraldehyde	Trypanosoma cruzi	-	in the absence of DMSO and methanol, in TEA-HCl buffer (pH 7.5) at 25�C	684403	2D-image
2.9	-	D-glyceraldehyde	Bos taurus	-	pH 8.0	287979	2D-image
0.0207	-	D-Glyceraldehyde 3-phosphate	Homo sapiens	-	pH 8.5, 25�C	695298	2D-image
0.053	-	D-Glyceraldehyde 3-phosphate	Haloarcula vallismortis	-	-	287907	2D-image
0.07	-	D-Glyceraldehyde 3-phosphate	Homo sapiens	-	-	287900	2D-image
0.081	-	D-Glyceraldehyde 3-phosphate	Euglena gracilis	-	-	287973	2D-image
0.082	-	D-Glyceraldehyde 3-phosphate	Oryctolagus cuniculus	-	-	287900	2D-image
0.087	-	D-Glyceraldehyde 3-phosphate	Homo sapiens	-	pH 7.3, enzyme isolated of sarcoma tissue	696174	2D-image
0.089	-	D-Glyceraldehyde 3-phosphate	Homo sapiens	-	pH 8.0, enzyme isolated of sarcoma tissue	696174	2D-image
0.09	-	D-Glyceraldehyde 3-phosphate	Geobacillus stearothermophilus, Oryctolagus cuniculus	-	-	287900	2D-image
0.09	-	D-Glyceraldehyde 3-phosphate	Oryctolagus cuniculus	-	-	287934	2D-image
0.1	-	D-Glyceraldehyde 3-phosphate	Streptomyces arenae	-	pentalenolactone-insensitive enzyme	287930	2D-image
0.11	-	D-Glyceraldehyde 3-phosphate	Homo sapiens	-	pH 8.8, enzyme isolated of sarcoma tissue	696174	2D-image
0.147	-	D-Glyceraldehyde 3-phosphate	Tetrahymena pyriformis	-	in 50 mM Tricine buffer, pH 8.5, 10 mM sodium arsenate, at 35�C	688066	2D-image
0.149	-	D-Glyceraldehyde 3-phosphate	Homo sapiens	-	pH 7.3, enzyme isolated of healthy patients	696174	2D-image
0.15	-	D-Glyceraldehyde 3-phosphate	Trypanosoma brucei	-	glyoxysomal enzyme	287900	2D-image
0.159	-	D-Glyceraldehyde 3-phosphate	Homo sapiens	-	pH 8.0, enzyme isolated of healthy patients	696174	2D-image
0.16	-	D-Glyceraldehyde 3-phosphate	Homo sapiens	-	pH 8.8, enzyme isolated of healthy patients	696174	2D-image
0.17	-	D-Glyceraldehyde 3-phosphate	Trypanosoma brucei	-	cytosolic enzyme	287900	2D-image
0.21	-	D-Glyceraldehyde 3-phosphate	Camelus dromedarius	-	in 50 mM tricine-NaOH buffer (pH 8.5) with 10 mM sodium arsenate, at 25�C	684127	2D-image
0.23	-	D-Glyceraldehyde 3-phosphate	Dissostichus mawsoni	-	-	287978	2D-image
0.24	-	D-Glyceraldehyde 3-phosphate	Sus scrofa	-	-	287966	2D-image
0.24	-	D-Glyceraldehyde 3-phosphate	Bos taurus	-	pH 8.0	287979	2D-image
0.25	-	D-Glyceraldehyde 3-phosphate	Streptomyces arenae	-	pentalenolactone-sensitive enzyme	287930	2D-image
0.29	-	D-Glyceraldehyde 3-phosphate	Escherichia coli	P0A9B2	mutant N313T/Y317G	287927	2D-image
0.3	-	D-Glyceraldehyde 3-phosphate	Sus scrofa	-	-	287934	2D-image
0.3	-	D-Glyceraldehyde 3-phosphate	Thermus thermophilus	-	-	287938, 287968	2D-image
0.31	-	D-Glyceraldehyde 3-phosphate	Geobacillus stearothermophilus	-	mutant enzyme W48F	287947	2D-image
0.31	-	D-Glyceraldehyde 3-phosphate	Staphylococcus aureus	-	wild type enzyme, pH 8.7, 25�C	712766	2D-image
0.32	-	D-Glyceraldehyde 3-phosphate	Staphylococcus aureus	-	mutant enzyme H178N, pH 8.7, 25�C	712766	2D-image
0.33	-	D-Glyceraldehyde 3-phosphate	Hypocrea koningii	-	GAPDH II	287908	2D-image
0.34	-	D-Glyceraldehyde 3-phosphate	Staphylococcus aureus	-	mutant enzyme C151S/H178N, pH 8.7, 25�C; mutant enzyme C151S, pH 8.7, 25�C	712766	2D-image
0.344	-	D-Glyceraldehyde 3-phosphate	Pinus sylvestris	-	-	287956	2D-image
0.385	-	D-Glyceraldehyde 3-phosphate	Bos taurus	-	pH 8.9	287979	2D-image
0.4	-	D-Glyceraldehyde 3-phosphate	Spinacia oleracea	-	-	287961	2D-image
0.42	-	D-Glyceraldehyde 3-phosphate	Escherichia coli	P0A9B2	mutant N313T	287927	2D-image
0.51	-	D-Glyceraldehyde 3-phosphate	Bacillus cereus	-	-	287974	2D-image
0.54	-	D-Glyceraldehyde 3-phosphate	Hypocrea koningii	-	GAPDH I	287908	2D-image
0.55	-	D-Glyceraldehyde 3-phosphate	Lactococcus lactis	-	30�C, pH 7.2	712937	2D-image
0.6	-	D-Glyceraldehyde 3-phosphate	Saccharomyces cerevisiae	-	-	287937	2D-image
0.762	-	D-Glyceraldehyde 3-phosphate	Cryptosporidium parvum	-	-	697008	2D-image
0.763	-	D-Glyceraldehyde 3-phosphate	Cryptosporidium parvum	-	wild type enzyme, in 50 mM triethanolamine, 50 mM Na2HPO4, 0.2 mM EDTA, pH 8.8, temperature not specified in the publication	711617	2D-image
0.77	-	D-Glyceraldehyde 3-phosphate	Homo sapiens	O14556	in 50 mM glycine, 50 mM potassium phosphate, 5 mM EDTA, pH 9.0	685294	2D-image
0.8	-	D-Glyceraldehyde 3-phosphate	Geobacillus stearothermophilus	-	wild-type enzyme	287947	2D-image
0.8	-	D-Glyceraldehyde 3-phosphate	Geobacillus stearothermophilus	-	mutant enzyme Y46G	287985	2D-image
0.88	-	D-Glyceraldehyde 3-phosphate	Escherichia coli	P0A9B2	mutant Y317A	287927	2D-image
0.89	-	D-Glyceraldehyde 3-phosphate	Escherichia coli	P0A9B2	wild-type enzyme	287927	2D-image
0.9	-	D-Glyceraldehyde 3-phosphate	Geobacillus stearothermophilus	-	mutant enzyme E276G	287985	2D-image
1	-	D-Glyceraldehyde 3-phosphate	Geobacillus stearothermophilus	-	mutant enzyme D186G and mutant enzyme S48G	287985	2D-image
1.1	-	D-Glyceraldehyde 3-phosphate	Geobacillus stearothermophilus	-	wild-type enzyme	287985	2D-image
1.45	-	D-Glyceraldehyde 3-phosphate	Neisseria meningitidis	-	recombinant enzyme, in 50 mM Tricine buffer (pH 8.5), at 25�C	688953	2D-image
2.05	-	D-Glyceraldehyde 3-phosphate	Escherichia coli	P0A9B2	mutant Y317G	287927	2D-image
0.0734	-	D-Glyceraldehyde-3-phosphate	Sardina pilchardus	-	in 50 mM tricine-NaOH buffer (pH 8.5) with 10 mM sodium arsenate, at 25�C	684121	2D-image
0.425	-	D-Glyceraldehyde-3-phosphate	Trypanosoma cruzi	-	free enzyme	684427	2D-image
0.5	-	D-Glyceraldehyde-3-phosphate	Trypanosoma cruzi	-	GAPDH immobilized on an octyl silica column	684427	2D-image
0.1	-	DL-glyceraldehyde	Homo sapiens	-	enzyme form E8.5, pH 7	287913	2D-image
0.119	-	erythrose 4-phosphate	Homo sapiens	-	enzyme form E8.5, pH 7	287913	2D-image
0.127	-	erythrose 4-phosphate	Homo sapiens	-	enzyme form E9.0, pH 7	287913	2D-image
0.0102	-	N6-(2-carboxyethyl)-NAD+	Thermus thermophilus	-	-	287926	2D-image
0.0674	-	N6-(2-carboxyethyl)-NAD+	Oryctolagus cuniculus	-	-	287926	2D-image
0.145	-	N6-(2-carboxyethyl)-NAD+	Geobacillus stearothermophilus	-	-	287926	2D-image
3.2e-05	-	NAD+	Cryptosporidium parvum	-	-	697008	2D-image
3.2e-05	-	NAD+	Cryptosporidium parvum	-	wild type enzyme, in 50 mM triethanolamine, 50 mM Na2HPO4, 0.2 mM EDTA, pH 8.8, temperature not specified in the publication	711617	2D-image
0.00519	-	NAD+	Thermus thermophilus	-	-	287926	2D-image
0.006	-	NAD+	Sus scrofa	-	-	287934	2D-image
0.0066	-	NAD+	Euglena gracilis	-	-	287973	2D-image
0.01	-	NAD+	Homo sapiens	-	enzyme form E8.5, pH 9	287913	2D-image
0.012	-	NAD+	Sus scrofa	-	-	287966	2D-image
0.013	-	NAD+	Oryctolagus cuniculus	-	-	287934	2D-image
0.014	-	NAD+	Hypocrea koningii	-	GAPDH I	287908	2D-image
0.0178	-	NAD+	Homo sapiens	-	pH 8.5, 25�C	695298	2D-image
0.018	-	NAD+	Oryctolagus cuniculus	-	-	287978	2D-image
0.02	-	NAD+	Homo sapiens	-	enzyme form E9.0, pH 9	287913	2D-image
0.022	-	NAD+	Homo sapiens	O14556	in 50 mM glycine, 50 mM potassium phosphate, 5 mM EDTA, pH 9.0	685294	2D-image
0.025	-	NAD+	Camelus dromedarius	-	in 50 mM tricine-NaOH buffer (pH 8.5) with 10 mM sodium arsenate, at 25�C	684127	2D-image
0.027	-	NAD+	Homo sapiens	-	enzyme form E6.8, pH 9	287913	2D-image
0.031	-	NAD+	Bos taurus	-	pH 8.9	287979	2D-image
0.035	-	NAD+	Homo sapiens	-	pH 7.3, enzyme isolated of sarcoma tissue	696174	2D-image
0.036	-	NAD+	Homo sapiens	-	pH 8.0, enzyme isolated of sarcoma tissue	696174	2D-image
0.0386	-	NAD+	Thermus thermophilus	-	poly(ethylene glycol)-bound	287926	2D-image
0.039	-	NAD+	Geobacillus stearothermophilus	-	-	287900	2D-image
0.04	-	NAD+	Trypanosoma brucei	-	cytosolic enzyme	287900	2D-image
0.04	-	NAD+	Geobacillus stearothermophilus	-	mutant enzyme S48G	287985	2D-image
0.04	-	NAD+	Homo sapiens	-	pH 8.8, enzyme isolated of sarcoma tissue	696174	2D-image
0.045	-	NAD+	Escherichia coli	P0A9B2	wild-type enzyme	287927	2D-image
0.046	-	NAD+	Hypocrea koningii	-	GAPDH II	287908	2D-image
0.047	-	NAD+	Homo sapiens	-	pH 8.8, enzyme isolated of healthy patients	696174	2D-image
0.05	-	NAD+	Geobacillus stearothermophilus, Homo sapiens	-	-	287900	2D-image
0.05	-	NAD+	Escherichia coli	P0A9B2	mutant Y317A	287927	2D-image
0.05	-	NAD+	Geobacillus stearothermophilus	-	wild-type enzyme	287985	2D-image
0.05	-	NAD+	Geobacillus stearothermophilus	-	pH 8.0, 25�C, tetrameric enzyme form, wild-type	654653	2D-image
0.0507	-	NAD+	Oryctolagus cuniculus	-	-	287926	2D-image
0.057	-	NAD+	Neisseria meningitidis	-	recombinant enzyme, in the presence of D-glyceraldehyde 3-phosphate, in 50 mM Tricine buffer (pH 8.5), at 25�C	688953	2D-image
0.058	-	NAD+	Escherichia coli	P0A9B2	mutant N313T/Y317G	287927	2D-image
0.06	-	NAD+	Oryctolagus cuniculus	-	-	287900	2D-image
0.06	-	NAD+	Geobacillus stearothermophilus	-	pH 8.0, 25�C, tetrameric enzyme form, mutant Y283V	654653	2D-image
0.062	-	NAD+	Pinus sylvestris	-	-	287956	2D-image
0.062	-	NAD+	Homo sapiens	-	pH 8.0, enzyme isolated of healthy patients	696174	2D-image
0.064	-	NAD+	Tetrahymena pyriformis	-	in 50 mM Tricine buffer, pH 8.5, 10 mM sodium arsenate, at 35�C	688066	2D-image
0.07	-	NAD+	Geobacillus stearothermophilus	-	mutant enzyme E276G	287985	2D-image
0.071	-	NAD+	Homo sapiens	-	pH 7.3, enzyme isolated of healthy patients	696174	2D-image
0.076	-	NAD+	Dissostichus mawsoni	-	-	287978	2D-image
0.0806	-	NAD+	Geobacillus stearothermophilus	-	-	287926	2D-image
0.088	-	NAD+	Neisseria meningitidis	-	recombinant enzyme, in 50 mM Tricine buffer (pH 8.5), in the presence of glyceraldehyde, at 25�C	688953	2D-image
0.092	-	NAD+	Sardina pilchardus	-	in 50 mM tricine-NaOH buffer (pH 8.5) with 10 mM sodium arsenate, at 25�C	684121	2D-image
0.097	-	NAD+	Huso huso	-	-	287942	2D-image
0.1	-	NAD+	Saccharomyces cerevisiae	-	-	287937	2D-image
0.1	-	NAD+	Thermus thermophilus	-	-	287938, 287968	2D-image
0.1	-	NAD+	Bacillus subtilis	-	wild-type enzyme	287980	2D-image
0.1	-	NAD+	Geobacillus stearothermophilus	-	pH 8.0, 25�C, tetrameric enzyme form, mutant W310F	654653	2D-image
0.109	-	NAD+	Neisseria meningitidis	-	recombinant enzyme, in 50 mM Tricine buffer (pH 8.5), in the presence of acetaldehyde, at 25�C	688953	2D-image
0.11	-	NAD+	Streptomyces arenae	-	pentalenolactone-sensitive enzyme	287930	2D-image
0.11	-	NAD+	Geobacillus stearothermophilus	-	mutant enzyme W84F	287947	2D-image
0.12	-	NAD+	Streptomyces arenae	-	pentalenolactone-insensitive enzyme	287930	2D-image
0.143	-	NAD+	Homo sapiens	-	enzyme form E6.6, pH 9	287913	2D-image
0.15	-	NAD+	Geobacillus stearothermophilus	-	wild-type enzyme	287947	2D-image
0.19	-	NAD+	Escherichia coli	P0A9B2	mutant N313T and Y317G	287927	2D-image
0.19	-	NAD+	Bos taurus	-	pH 8.0	287979	2D-image
0.2	-	NAD+	Geobacillus stearothermophilus	-	pH 8.0, 25�C, tetrameric enzyme form, mutant D282G	654653	2D-image
0.2	-	NAD+	Lactococcus lactis	-	30�C, pH 7.2	712937	2D-image
0.23	-	NAD+	Geobacillus stearothermophilus	-	poly(ethylene glycol)-bound NAD+	287926	2D-image
0.25	-	NAD+	Spinacia oleracea	-	-	287961	2D-image
0.258	-	NAD+	Trypanosoma cruzi	-	free enzyme	684427	2D-image
0.27	-	NAD+	Bacillus cereus	-	-	287974	2D-image
0.29	-	NAD+	Geobacillus stearothermophilus	-	pH 8.0, 25�C, tetrameric enzyme form, mutant T34Q/T39S/L43Q	654653	2D-image
0.33	-	NAD+	Geobacillus stearothermophilus	-	mutant enzyme S186G	287985	2D-image
0.45	-	NAD+	Trypanosoma brucei	-	glyoxysomal enzyme	287900	2D-image
0.5	-	NAD+	Bacillus subtilis	-	mutant enzyme D32A	287980	2D-image
0.674	-	NAD+	Trypanosoma cruzi	-	GAPDH immobilized on an octyl silica column	684427	2D-image
0.75	-	NAD+	Bacillus subtilis	-	mutant enzyme D32A/L187N	287980	2D-image
1	-	NAD+	Bacillus subtilis	-	mutant enzyme L187N	287980	2D-image
2.46	-	NAD+	Geobacillus stearothermophilus	-	poly(ethylene glycol)-bound NAD+	287926	2D-image
2.5	-	NAD+	Geobacillus stearothermophilus	-	pH 8.0, 25�C, tetrameric enzyme form, mutant N313T	654653	2D-image
312	-	NAD+	Staphylococcus aureus	-	mutant enzyme C151S/H178N, pH 8.7, 25�C	712766	2D-image
316	-	NAD+	Staphylococcus aureus	-	wild type enzyme, pH 8.7, 25�C	712766	2D-image
320	-	NAD+	Staphylococcus aureus	-	mutant enzyme H178N, pH 8.7, 25�C	712766	2D-image
322	-	NAD+	Staphylococcus aureus	-	mutant enzyme C151S, pH 8.7, 25�C	712766	2D-image
0.0033	-	NADH	Oryctolagus cuniculus	-	-	287934	2D-image
0.007	-	NADH	Trypanosoma brucei	-	cytosolic enzyme	287900	2D-image
0.01	-	NADH	Homo sapiens	-	-	287900	2D-image
0.012	-	NADH	Geobacillus stearothermophilus, Oryctolagus cuniculus	-	-	287900	2D-image
0.02	-	NADH	Trypanosoma brucei	-	glyoxysomal enzyme	287900	2D-image
0.021	-	NADH	Euglena gracilis	-	-	287973	2D-image
0.023	-	NADH	Sus scrofa	-	-	287934, 287966	2D-image
1.7	-	NADP+	Bacillus subtilis	-	mutant enzyme D32A/L187N	287980	2D-image
7.1	-	NADP+	Bacillus subtilis	-	mutant enzyme D32A and L187N	287980	2D-image
0.2	-	phosphate	Hypocrea koningii	-	isoenzyme I and II	287908	2D-image
0.53	-	phosphate	Escherichia coli	P0A9B2	wild-type enzyme	287927	2D-image
1.5	-	phosphate	Saccharomyces cerevisiae	-	-	287937	2D-image
2.54	-	phosphate	Staphylococcus aureus	-	wild type enzyme, pH 8.7, 25�C	712766	2D-image
3.03	-	phosphate	Staphylococcus aureus	-	mutant enzyme H178N, pH 8.7, 25�C	712766	2D-image
3.09	-	phosphate	Staphylococcus aureus	-	mutant enzyme C151S, pH 8.7, 25�C	712766	2D-image
3.4	-	phosphate	Bos taurus	-	pH 8.9	287979	2D-image
3.68	-	phosphate	Staphylococcus aureus	-	mutant enzyme C151S/H178N, pH 8.7, 25�C	712766	2D-image
4	-	phosphate	Homo sapiens	-	pH 8.8, enzyme isolated of healthy patients	696174	2D-image
4.8	-	phosphate	Escherichia coli	P0A9B2	mutant enzyme Y317A	287927	2D-image
6.9	-	phosphate	Escherichia coli	P0A9B2	mutant enzyme N313T/Y317G	287927	2D-image
7	-	phosphate	Geobacillus stearothermophilus	-	mutant enzyme E276G and mutant enzyme S48G	287985	2D-image
7.15	-	phosphate	Bos taurus	-	pH 8.0	287979	2D-image
8	-	phosphate	Geobacillus stearothermophilus	-	mutant enzyme Y46G	287985	2D-image
8.3	-	phosphate	Geobacillus stearothermophilus	-	wild-type enzyme	287947	2D-image
9.6	-	phosphate	Geobacillus stearothermophilus	-	mutant enzyme W84F	287947	2D-image
9.9	-	phosphate	Homo sapiens	-	pH 8.8, enzyme isolated of sarcoma tissue	696174	2D-image
18	-	phosphate	Geobacillus stearothermophilus	-	mutant enzyme D186G	287985	2D-image
20	-	phosphate	Escherichia coli	P0A9B2	mutant enzyme N313T	287927	2D-image
35.1	-	phosphate	Escherichia coli	P0A9B2	mutant enzyme Y317G	287927	2D-image
37	-	phosphate	Geobacillus stearothermophilus	-	wild-type enzyme	287985	2D-image
0.0435	-	Thio-NAD+	Oryctolagus cuniculus	-	-	287978	2D-image
0.235	-	Thio-NAD+	Dissostichus mawsoni	-	-	287978	2D-image
0.2	-	glyceraldehyde	Neisseria meningitidis	-	recombinant enzyme, at 25�C	688953	2D-image
additional information	-	additional information	Geobacillus stearothermophilus	-	-	287909	-
additional information	-	additional information	Glycine max	-	-	287920	-
additional information	-	additional information	Homo sapiens	-	-	287932, 287950	-
additional information	-	additional information	Oryctolagus cuniculus	-	activity depends non-linearly on protein concentration in the range 0.00003-0.003 mM. With increasing concentrations the apparently hyperbolic substrate saturation curves turn into sigmoidal ones	287953	-

TURNOVER NUMBER [1/s]	TURNOVER NUMBER MAXIMUM[1/s]	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
4.03	-	acetaldehyde	Neisseria meningitidis	-	recombinant enzyme, in 50 mM Tricine buffer (pH 8.5), at 25�C	688953	2D-image
71.4	-	D-glyceraldehyde	Trypanosoma cruzi	-	in the absence of DMSO and methanol, in TEA-HCl buffer (pH 7.5) at 25�C	684403	2D-image
118	-	D-glyceraldehyde	Trypanosoma cruzi	-	in the presence of 5% (v/v) DMSO, in TEA-HCl buffer (pH 7.5) at 25�C	684403	2D-image
442	-	D-glyceraldehyde	Trypanosoma cruzi	-	in the presence of 5% (v/v) methanol, in TEA-HCl buffer (pH 7.5) at 25�C	684403	2D-image
0.002	-	D-Glyceraldehyde 3-phosphate	Staphylococcus aureus	-	mutant enzyme C151S/H178N, pH 8.7, 25�C	712766	2D-image
0.05	-	D-Glyceraldehyde 3-phosphate	Staphylococcus aureus	-	mutant enzyme H178N, pH 8.7, 25�C	712766	2D-image
0.09	-	D-Glyceraldehyde 3-phosphate	Staphylococcus aureus	-	mutant enzyme C151S, pH 8.7, 25�C	712766	2D-image
16.7	-	D-Glyceraldehyde 3-phosphate	Saccharomyces cerevisiae	-	-	287937	2D-image
24.51	-	D-Glyceraldehyde 3-phosphate	Neisseria meningitidis	-	recombinant enzyme, in 50 mM Tricine buffer (pH 8.5), at 25�C	688953	2D-image
46	-	D-Glyceraldehyde 3-phosphate	Geobacillus stearothermophilus	-	mutant enzyme W84F	287947	2D-image
50	-	D-Glyceraldehyde 3-phosphate	Spinacia oleracea	-	-	287935	2D-image
70	-	D-Glyceraldehyde 3-phosphate	Geobacillus stearothermophilus	-	-	287947	2D-image
70	-	D-Glyceraldehyde 3-phosphate	Staphylococcus aureus	-	wild type enzyme, pH 8.7, 25�C	712766	2D-image
0.1	-	NAD+	Geobacillus stearothermophilus	-	pH 8.0, 25�C, tetrameric enzyme form, mutant D282G; pH 8.0, 25�C, tetrameric enzyme form, mutant T34Q/T39S/L43Q	654653	2D-image
1	-	NAD+	Geobacillus stearothermophilus	-	pH 8.0, 25�C, tetrameric enzyme form, mutant N313T	654653	2D-image
2.5	-	NAD+	Bacillus subtilis	-	mutant enzyme D32A/L187N	287980	2D-image
51	-	NAD+	Geobacillus stearothermophilus	-	pH 8.0, 25�C, tetrameric enzyme form, mutant W310F	654653	2D-image
65	-	NAD+	Geobacillus stearothermophilus	-	pH 8.0, 25�C, tetrameric enzyme form, wild-type	654653	2D-image
70	-	NAD+	Bacillus subtilis	-	wild-type enzyme and mutant enzyme L187N	287980	2D-image
70	-	NAD+	Lactococcus lactis	-	30�C, pH 7.2	712937	2D-image
73	-	NAD+	Geobacillus stearothermophilus	-	pH 8.0, 25�C, tetrameric enzyme form, mutant Y283V	654653	2D-image
112	-	NAD+	Bacillus subtilis	-	mutant enzyme L187N	287980	2D-image
2	-	NADP+	Bacillus subtilis	-	mutant enzyme D32A	287980	2D-image
8	-	NADP+	Bacillus subtilis	-	mutant enzyme L187N	287980	2D-image
17.7	-	NADP+	Bacillus subtilis	-	mutant enzyme D32A/L187N	287980	2D-image
4.35	-	glyceraldehyde	Neisseria meningitidis	-	recombinant enzyme, in 50 mM Tricine buffer (pH 8.5), at 25�C	688953	2D-image
additional information	-	additional information	Escherichia coli	P0A9B2	-	287927	-
additional information	-	additional information	Geobacillus stearothermophilus	-	-	287985	-

kcat/KM VALUE [1/mMs^-1]	kcat/KM VALUE [1/mMs^-1] Maximum	SUBSTRATE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
No entries in this field

Ki VALUE [mM]	Ki VALUE [mM] Maximum	INHIBITOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
0.004	-	2-(dodec-1-en-1-yl)-6-hydroxybenzoic acid	Trypanosoma cruzi	-	pH 8.9, 25�C, substrate D-glyceraldehyde 3-phosphate	696735	-
0.005	-	2-(dodec-1-en-1-yl)-6-hydroxybenzoic acid	Trypanosoma cruzi	-	pH 8.9, 25�C, substrate NAD+	696735	-
0.002	-	2-pentadecyl-6-hydroxybenzoic acid	Trypanosoma cruzi	-	pH 8.9, 25�C, substrate D-glyceraldehyde 3-phosphate	696735	-
0.004	-	2-pentadecyl-6-hydroxybenzoic acid	Trypanosoma cruzi	-	pH 8.9, 25�C, substrate NAD+	696735	-
0.145	-	iodoacetate	Lactococcus lactis	-	30�C, pH 7.2	712937	2D-image
0.02	-	NADH	Lactococcus lactis	-	30�C, pH 7.2, competitive inhibitor, no effect on the maximum velocity	712937	2D-image

IC50 VALUE [mM]	IC50 VALUE [mM] Maximum	INHIBITOR	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	IMAGE
0.161	-	(8E)-C15:1-anacardic acid	Trypanosoma cruzi	P22513	pH 7.5, 25�C	696747	2D-image
0.069	-	(8E,11E)-C15:2-anacardic acid	Trypanosoma cruzi	P22513	pH 7.5, 25�C	696747	2D-image
0.038	-	(8E,11E,14E)-C15:3-anacardic acid	Trypanosoma cruzi	P22513	pH 7.5, 25�C	696747	2D-image
0.055	-	2-(dodec-1-en-1-yl)-6-hydroxybenzoic acid	Trypanosoma cruzi	-	pH 8.9, 25�C	696735	-
0.028	-	2-pentadecyl-6-hydroxybenzoic acid	Trypanosoma cruzi	-	pH 8.9, 25�C	696735	-
4.6	-	Cd2+	Camelus dromedarius	-	at 25�C	684127	2D-image
22.7	-	Cu2+	Camelus dromedarius	-	at 25�C	684127	2D-image
0.14	-	guajaverin	Trypanosoma cruzi	P22513	pH 7.5, 25�C	696747	2D-image
6.2	-	Hg2+	Camelus dromedarius	-	at 25�C	684127	2D-image
0.075	-	hydrogen peroxide	Tetrahymena pyriformis	-	in 50 mM Tricine buffer, pH 8.5, 10 mM sodium arsenate, at 35�C	688066	2D-image
34.6	-	K+	Camelus dromedarius	-	at 25�C	684127	2D-image
0.149	-	mangiferin	Trypanosoma cruzi	P22513	pH 7.5, 25�C	696747	2D-image
25.8	-	Mn2+	Camelus dromedarius	-	at 25�C	684127	2D-image
0.142	-	quercetin	Trypanosoma cruzi	P22513	pH 7.5, 25�C	696747	2D-image
0.05	-	sodium nitroprusside	Tetrahymena pyriformis	-	in 50 mM Tricine buffer, pH 8.5, 10 mM sodium arsenate, at 35�C	688066	2D-image
0.046	-	tiliroside	Trypanosoma cruzi	P22513	pH 7.5, 25�C	696747	2D-image
14.2	-	Zn2+	Camelus dromedarius	-	at 25�C	684127	2D-image

SPECIFIC ACTIVITY [µmol/min/mg]	SPECIFIC ACTIVITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
0.01	-	Solanum tuberosum	Q8LK04	transgenic line 7, source leaves	675137
0.025	-	Solanum tuberosum	Q8LK04	transgenic line 56, source leaves	675137
0.035	-	Solanum tuberosum	Q8LK04	wild-type, untransformed control, source leaves	675137
0.042	-	Solanum tuberosum	Q8LK04	transgenic line 97, source leaves	675137
0.1	-	Solanum tuberosum	Q8LK04	transgenic line 7, growing tubers; transgenic line 97, growing tubers	675137
0.1767	-	Entamoeba histolytica	Q27646	crude extract, at 30 �C	686607
0.18	-	Solanum tuberosum	Q8LK04	transgenic line 7, sink leaves	675137
0.2	-	Solanum tuberosum	Q8LK04	transgenic line 56, stolons; transgenic line 7, stolons; transgenic line 97, sink leaves; transgenic line 97, stolons	675137
0.25	-	Solanum tuberosum	Q8LK04	transgenic line 97, roots	675137
0.3	-	Solanum tuberosum	Q8LK04	transgenic line 56, growing tubers; transgenic line 7, roots	675137
0.35	-	Solanum tuberosum	Q8LK04	transgenic line 56, sink leaves	675137
0.46	-	Sardina pilchardus	-	crude extract	684121
0.5	-	Solanum tuberosum	Q8LK04	transgenic line 56, roots	675137
0.52	-	Neisseria meningitidis	-	recombinant enzyme from crude extract, in 50 mM Tricine buffer (pH 8.5), at 25�C	688953
0.72	-	Tetrahymena pyriformis	-	cell-free extract, in 50 mM Tricine buffer, pH 8.5, 10 mM sodium arsenate, at 25�C	688066
0.75	-	Solanum tuberosum	Q8LK04	transgenic line 7, stem	675137
0.891	-	Lactococcus lactis ssp. cremoris	-	strain MG1363, pH 6.6	674222
0.9	-	Solanum tuberosum	Q8LK04	wild-type, untransformed control, growing tubers	675137
1.1	-	Solanum tuberosum	Q8LK04	wild-type, untransformed control, roots	675137
1.1	-	Homo sapiens	-	pH 8.5, 25�C	695298
1.2	-	Solanum tuberosum	Q8LK04	wild-type, untransformed control, sink leaves	675137
1.38	-	Lactococcus lactis ssp. cremoris	-	strain MG1363, pH 5.0	674222
1.5	-	Solanum tuberosum	Q8LK04	transgenic line 56, stem; transgenic line 97, stem	675137
1.68	-	Camelus dromedarius	-	crude extract, at 25�C	684127
1.75	-	Solanum tuberosum	Q8LK04	wild-type, untransformed control, stem	675137
1.8	-	Solanum tuberosum	Q8LK04	wild-type, untransformed control, stolons	675137
2.16	-	Neisseria meningitidis	-	crude extract, at 25�C	688953
3.01	-	Lactococcus lactis ssp. cremoris	-	strain MG1363, pH 4.7	674222
3.6	-	Pseudomonas sp.	A7LHM7	23�C, pH 7.5, recombinant His-tagged enzyme	698639
3.92	-	Lactococcus lactis ssp. cremoris	-	relA acid-resistant mutant, pH 6.6	674222
4.313	-	Lactococcus lactis ssp. cremoris	-	strain MG1363, pH 4.4	674222
4.49	-	Lactococcus lactis ssp. cremoris	-	relA acid-resistant mutant, pH 4.7	674222
5.4	-	Thermus thermophilus	-	crystalline enzyme	287938
5.4	-	Thermus thermophilus	-	-	287968
8.6	-	Homo sapiens	-	enzyme form E6.6	287913
13	-	Homo sapiens	-	enzyme form E6.8	287913
16	-	Thermus aquaticus	-	apoenzyme	287946
16.5	-	Thermus aquaticus	-	holoenzyme	287946
25.1	-	Neisseria meningitidis	-	recombinant enzyme after 48.3fold purification, in 50 mM Tricine buffer (pH 8.5), at 25�C	688953
26.4	-	Tetrahymena pyriformis	-	after 36.7fold purifictaion, in 50 mM Tricine buffer, pH 8.5, 10 mM sodium arsenate, at 25�C	688066
26.69	-	Geobacillus stearothermophilus	-	-	287946
30	-	Arabidopsis thaliana	P25858, Q56WJ4	purified isozyme GapC1	689409
33	-	Streptomyces arenae	-	pentalenolactone-insensitive enzyme	287930
33	-	Arabidopsis thaliana	P25858, Q56WJ4	purified GapC2 C159S mutant isozyme	689409
35.75	-	Sardina pilchardus	-	after 77.71fold purification	684121
41.5	-	Haloarcula vallismortis	-	-	287907
44	-	Thermotoga maritima	-	-	287906
45.1	-	Camelus dromedarius	-	after 26.8fold purification, at 25�C	684127
50.5	-	Bos taurus	-	-	287979
60	-	Apis mellifera	-	-	287971
60	-	Arabidopsis thaliana	P25858, Q56WJ4	purified isozyme GapC2	689409
62	-	Homo sapiens	O14556	-	685294
68.1	-	Homo sapiens	-	enzyme from liver	287932
75	-	Sus scrofa	-	enzyme from liver	3246
80	-	Sus scrofa	-	enzyme from muscle	3246
80.4	-	Spinacia oleracea	-	-	287935
90	-	Spinacia oleracea	-	purified enzyme	689409
91.4	-	Bos taurus	-	-	287905
94	-	Oryctolagus cuniculus	-	-	670846
97.9	-	Oryctolagus cuniculus	-	-	287969
98	-	Homo sapiens	-	-	287945
100	-	Oryctolagus cuniculus, Saccharomyces cerevisiae	-	purified enzyme	689409
109	-	Bacillus coagulans	-	-	287963
112	-	Streptomyces arenae	-	pentalenolactone-sensitive enzyme	287930
125	-	Hypocrea koningii	-	-	287908
145	-	Glycine max	-	-	287902
150	-	Sus scrofa	-	-	287966
150	-	Geobacillus stearothermophilus	-	purified enzyme	672306
158	-	Homo sapiens	-	enzyme form E8.5	287913
190	-	Cavia porcellus	-	-	670846
195	-	Bos taurus	-	brain	287911
211	-	Bos taurus	-	muscle	287911
219	-	Bos taurus	-	heart	287911
620	-	Homo sapiens	-	enzyme form E9.0	287913
additional information	-	Geobacillus stearothermophilus, Trypanosoma brucei	-	-	287900
additional information	-	Caenorhabditis elegans	-	-	287928
additional information	-	Pisum sativum	-	-	287936
additional information	-	Saccharomyces cerevisiae	-	-	287937, 287948
additional information	-	Mus musculus	-	-	287958
additional information	-	Spinacia oleracea	-	-	287961
additional information	-	Geobacillus stearothermophilus	-	-	287970
additional information	-	Euglena gracilis	-	-	287973
additional information	-	Bacillus cereus	-	-	287974

pH OPTIMUM	pH MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
7	-	Homo sapiens	-	enzyme form E6.8, two pH optima: pH 7.0 and pH 8.5, with activity between pH 7.5 and pH 8.0 being rather low	287913
7	-	Bos taurus	-	reduction of 3-phospho-D-glyceroyl phosphate, triethanolamine buffer	287979
7.2	7.3	Homo sapiens	-	reaction with 3-phospho-D-glyceroylphosphate	287932
7.2	-	Lactococcus lactis ssp. cremoris	-	activity assay	674222
7.3	8.8	Homo sapiens	-	enzyme isolated from sarcoma tissue, broad	696174
7.4	8.1	Bos taurus	-	reduction of 1,3-diphosphogycerate, Tris buffer	287979
7.4	-	Cavia porcellus, Oryctolagus cuniculus	-	activity assay	670846
7.5	-	Solanum tuberosum	Q8LK04	activity assay	675137
7.8	8.1	Bos taurus	-	oxidation of D-glyceraldehyde 3-phosphate, Tris buffer and triethanolamine buffer	287979
7.8	-	Camelus dromedarius	-	-	684127
7.9	8.9	Euglena gracilis	-	-	287973
7.9	-	Arabidopsis thaliana	-	activity assay	673587
8	8.3	Homo sapiens	-	reaction with D-glyceraldehyde 3-phosphate	287932
8	8.3	Homo sapiens	O14556	-	685294
8	8.5	Trypanosoma brucei	-	-	287901
8	9	Thermotoga maritima	-	oxidation of D-glyceraldehyde 3-phosphate, at 40�C	287906
8	9	Homo sapiens	O14556	-	685294
8	-	Escherichia coli	-	50 mM Tris-chloride buffer and triethanolamine-chloride buffer	287977
8	-	Sardina pilchardus	-	-	684121
8.3	-	Thermus thermophilus	-	-	287938, 287968
8.4	8.8	Sus scrofa	-	-	287934, 287966
8.5	9	Trypanosoma brucei	-	cytosolic enzyme and enzyme from glyoxysomes	287900
8.5	9	Pisum sativum	-	-	287936
8.5	-	Homo sapiens	-	enzyme form E6.8, two pH optima: pH 7.0 and pH 8.5, with activity between pH 7.5 and pH 8.0 being rather low	287913
8.5	-	Saccharomyces cerevisiae	-	phosphorolysis and arsenolysis	287937
8.5	-	Bos taurus	-	oxidation of D-glyceraldehyde 3-phosphate, glycine buffer	287979
8.5	-	Streptococcus pyogenes	P0C0G6	the enzymatic activity of cell-associated Plr-SDH-GAPDH complex is measured	674559
8.5	-	Homo sapiens	-	-	695298
8.6	-	Haloarcula vallismortis	-	-	287907
8.6	-	Bacillus coagulans	-	-	287963
8.6	-	Bacillus cereus	-	-	287974
8.6	-	Homo sapiens	-	enzyme isolated from normal tissue, sharp optimum	696174
8.7	-	Staphylococcus aureus	-	-	712766
8.8	-	Escherichia coli	-	50 mM sodium diphosphate buffer	287977
8.9	9.1	Spinacia oleracea	-	-	287935
8.9	-	Hypocrea koningii	-	GAPDH I and GAPDH II	287908
8.9	-	Oryctolagus cuniculus	-	activity assay	672402
9	10	Neisseria meningitidis	-	-	688953
9.1	-	Apis mellifera	-	-	287971
9.3	-	Pseudomonas aeruginosa	-	-	287919
9.8	-	Homo sapiens	-	enzyme form E8.5, D-glyceraldehyde 3-phosphate	287913
10	-	Geobacillus stearothermophilus	-	-	287970
10	-	Geobacillus stearothermophilus	-	activity assay	672306

pH RANGE	pH RANGE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
5.5	9	Trypanosoma brucei	-	pH 5.5: about 50% of maximal activity, pH 9.0: about 55% of maximal activity, cytosolic enzyme	287901
6	10	Thermotoga maritima	-	pH 6.0: about 50% of maximal activity, pH 10.0: about 60% of maximal activity, oxidation of D-glyceraldehyde 3-phosphate, at 40�C	287906
7	8.5	Camelus dromedarius	-	-	684127
7	8.8	Thermus thermophilus	-	pH 7.0: about 55% of maximal activity, pH 8.8: about 95% of maximal activity	287968
7	9	Trypanosoma brucei	-	about 40% of maximal activity at pH 7.0 and at pH 9.0, glyoxysomal enzyme	287901
7	9.5	Bacillus cereus	-	pH 7.0: about 70% of maximal activity, pH 9.5: about 55% of maximal activity	287974
7.4	9.6	Sus scrofa	-	about 50% of maximal activity at pH 7.4 and at ph 9.6	287934, 287966
7.5	8.5	Escherichia coli	-	about 85% of maximal activity at pH 7.5 and pH 8.5, 50 mM Tris-chloride buffer. About 75% of maximal activity at pH 7.5 and pH 8.5, 50 mM triethanolamine-chloride buffer	287977
7.5	8.5	Sardina pilchardus	-	-	684121
8.3	9.3	Escherichia coli	-	85% of maximal activity at pH 8.3 and pH 9.3, 50 mM sodium diphosphate buffer	287977
8.8	-	Homo sapiens	-	above, sharp decline in activity for enzyme of patients with chronic myeloid leukemia, no similar sharp decline for enzyme of healthy subjects	696174

TEMPERATURE OPTIMUM	TEMPERATURE OPTIMUM MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
25	-	Oryctolagus cuniculus	-	activity assay	672402
25	-	Arabidopsis thaliana	-	activity assay	673587
25	-	Staphylococcus aureus	-	-	712766
28	32	Camelus dromedarius	-	-	684127
30	-	Euglena gracilis	-	-	287973
30	-	Lactococcus lactis ssp. cremoris	-	activity assay	674222
30	-	Sardina pilchardus	-	-	684121
35	-	Tetrahymena pyriformis	-	-	688066
37	-	Streptococcus pyogenes	P0C0G6	the enzymatic activity of cell-associated Plr-SDH-GAPDH complex is measured	674559
43	-	Homo sapiens	-	-	695298
64	-	Neisseria meningitidis	-	-	688953

TEMPERATURE RANGE	TEMPERATURE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
28	32	Sardina pilchardus	-	-	684121
45	-	Oryctolagus cuniculus	-	100% thermoinactivation of GAPDH in the absence and in the presence of GroEL after 80 and 60 min, respectively, at 45�C	672402

pI VALUE	pI VALUE MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
5.6	-	Lentinus polychrous	D3K1B6	calculated from amino acid sequence	710790
6.2	-	Oplegnathus fasciatus	B5AAJ5, B5AAJ6	calculated	698019
6.3	-	Neisseria meningitidis	-	isoelectric focusing	688953
6.4	-	Entamoeba histolytica	Q27646	isoelectric focusing	686607
6.5	-	Ascaris suum	Q95YJ4	-	656745
6.8	-	Paracoccidioides brasiliensis	Q8X1X3	one of two identified isoforms	673986
6.9	-	Pleurodeles waltl	Q8AXB5	isoelectric focusing	655270
7	7.5	Mammalia	-	GAPDHc, localized in the cytosol	675023
7	-	Paracoccidioides brasiliensis	Q8X1X3	one of two identified isoforms	673986
7.1	-	Xenopus laevis	-	isoelectric focusing	655270
7.2	-	Camelus dromedarius	-	isoelectric focusing	684127
7.6	-	Plasmodium falciparum	Q8IKK7	calculated	677046
7.9	-	Sardina pilchardus	-	isoelectric focusing	684121
8.4	-	Oryctolagus cuniculus	-	-	670846
8.6	8.7	Mammalia	-	GAPDHn, localized in the nucleus	675023
8.7	-	Oplegnathus fasciatus	B5AAJ5, B5AAJ6	calculated	698019
8.8	-	Tetrahymena pyriformis	-	isoelectric focusing	688066
9.1	-	Dunaliella salina	B1PL92	calculated	697508

SOURCE TISSUE	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE	SOURCE
A-549 cell	Homo sapiens	-	-	699592
bacteroid	Bradyrhizobium japonicum	-	-	287902
BHK-21 cell	Mesocricetus auratus	-	infected with Japanese encephalitis virus	699035
blood	Mus musculus	-	-	688791
brain	Bos taurus	-	-	287911
brain	Homo sapiens	-	-	287913, 287932
brain	Mus musculus	-	-	688791
brain	Oplegnathus fasciatus	B5AAJ5, B5AAJ6	strong est expression; weak expression	698019
brain cortex	Mus musculus, Rattus norvegicus	-	-	685334
C28/I2 cell	Homo sapiens	-	human juvenile costal chondrocyte cell	684972
cerebellum	Mus musculus, Rattus norvegicus	-	-	685334
cerebral cortex	Bos taurus	-	-	656594
commercial preparation	Oryctolagus cuniculus	-	-	698989
coronary artery smooth muscle	Homo sapiens	-	-	700503
COS-7 cell	Homo sapiens	-	-	684972
Ehrlich ascites carcinoma cell	Mus musculus	-	-	287957
endothelial cell	Homo sapiens	-	lung, microvascular	654850
epithelial cell	Homo sapiens	-	-	673706
erythrocyte	Homo sapiens	-	-	287900, 287910, 287932, 287945, 287950, 657393, 673629, 685025, 688250, 695298, 712640
eye	Bos taurus	-	rod outer segment	287905
eye	Oplegnathus fasciatus	B5AAJ5, B5AAJ6	strong expression; weak expression; weak expression	698019
fibroblast	Homo sapiens	-	in both fetal and senior cells, considerable GAPDH is present in intracellular domains characterized by significantly reduced catalysis. Human cells contain significant intracellular levels of enzymatically inactive GAPDH which is age-independent	654849
fin	Oplegnathus fasciatus	B5AAJ5, B5AAJ6	moderate expression; poor expression	698019
flower	Arabidopsis thaliana	P25858, Q56WJ4	-	689409
fruitbody	Lentinus polychrous	D3K1B6	-	710790
gill	Oplegnathus fasciatus	B5AAJ5, B5AAJ6	moderate expression; weak expression	698019
HCT-116 cell	Homo sapiens	-	-	676259
heart	Bos taurus	-	-	287911
heart	Homo sapiens	-	-	287932
heart	Rattus norvegicus	-	-	674503
heart	Mus musculus	-	-	688791
heart	Oplegnathus fasciatus	B5AAJ5, B5AAJ6	moderate expression; moderate expression	698019
HEK-293 cell	Homo sapiens	-	infected with Japanese encephalitis virus	699035
HEK-293 cell	Homo sapiens	-	-	700503
HEK-293T cell	Homo sapiens	-	-	684972
HeLa cell	Homo sapiens	-	-	684972, 696824
hepatocyte	Rattus norvegicus	-	-	688949
hypodermis	Ascaris suum	Q95YJ4	-	656745
intestinal epithelium	Ascaris suum	Q95YJ4	-	656745
intestine	Oplegnathus fasciatus	B5AAJ5, B5AAJ6	moderate expression; moderate expression	698019
KB cell	Homo sapiens	-	KB cell line ATCC CCL17	673706
kidney	Homo sapiens	-	-	287932
kidney	Mus musculus	-	-	287958, 688791
kidney	Oplegnathus fasciatus	B5AAJ5, B5AAJ6	moderate expression; strong expression	698019
leaf	Spinacia oleracea	-	-	287935, 287961, 689409
leaf	Arabidopsis thaliana	-	expression slowly decreases in the dark and is stable in sucrose-treated leaves	656414
leaf	Solanum tuberosum	Q8LK04	-	675137
leaf	Arabidopsis thaliana	P25858, Q56WJ4	-	689409
leukemic monocyte cell line	Homo sapiens	-	-	686867
liver	Homo sapiens	-	-	287913, 287932, 671066, 685292, 685294, 699592
liver	Rattus norvegicus	-	-	287913, 656072, 657393
liver	Sus scrofa	-	-	287934, 287966, 3246
liver	Mus musculus	-	-	287958, 688791
liver	Bos taurus	-	-	287979
liver	Oplegnathus fasciatus	B5AAJ5, B5AAJ6	poor expression; predominant expression	698019
lung	Homo sapiens	-	-	287932
lung	Homo sapiens	-	microvascular endothelial cell	654850
muscle	Oryctolagus cuniculus	-	-	287900, 287904, 287926, 287933, 287953, 287957, 287959, 287965, 287967, 287969, 287972, 287978, 287981, 288344, 654080, 654437, 670846, 672402, 673706, 685292, 686305, 686481, 686849, 687602, 689409, 696421, 698989
muscle	Homo sapiens	-	-	287900, 287967
muscle	Bos taurus	-	-	287911, 287967
muscle	Sus scrofa	-	-	287914, 287967, 3246
muscle	Caenorhabditis elegans	-	isoenzyme 2 is body-wall muscle specific activity located within the actin-containing I and A zones of the nematode�s sarcomers	287928
muscle	Rattus norvegicus	-	-	287929, 287967, 686305
muscle	Homo sapiens	-	skeletal muscle	287932
muscle	Huso huso	-	-	287942
muscle	Palinurus vulgaris	-	tail muscle	287951
muscle	Mus musculus	-	-	287957, 287958
muscle	Rattus norvegicus	-	skeletal muscle	287960
muscle	Acipenser sp.	-	-	287964, 287967
muscle	Canis lupus familiaris, Felis catus, Gallus gallus, Hippoglossus sp., Lobster, Meleagris gallopavo	-	-	287967
muscle	Apis mellifera	-	flight muscle, located at or near the Z-band cross-striations	287971
muscle	Dissostichus mawsoni	-	skeletal muscle	287978
muscle	Oryctolagus cuniculus	-	of back and hind legs	3247
muscle	Ascaris suum	Q95YJ4	-	656745
muscle	Cavia porcellus	-	-	670846
muscle	Oplegnathus fasciatus	B5AAJ5, B5AAJ6	poor expression; predominant expression	698019
mycelium	Emericella nidulans	-	-	287903
mycelium	Lentinus polychrous	D3K1B6	-	710790
myocyte	Rattus norvegicus	-	ventricular myocytes isolated from adult Sprague-Dawley rats	674503
neuroblastoma cell	Homo sapiens	-	-	687602
neuron	Mus musculus	-	striatal	287982
nodule	Glycine max	-	nitrogen fixing	287902
nodule	Glycine max	-	-	287920
NOSE1 cell	Homo sapiens	-	normal human ovarian surface epithelial cell line	700133
ovarian carcinoma cell	Homo sapiens	-	-	700133
ovary	Ascaris suum	Q95YJ4	-	656745
placenta	Homo sapiens	-	-	671070
seed	Pisum sativum	-	-	287936, 287967
SH-SY5Y cell	Homo sapiens	-	-	687602
silique	Arabidopsis thaliana	P25858, Q56WJ4	-	689409
skeletal muscle	Homo sapiens	-	for structural comparison	671066
skeletal muscle	Sardina pilchardus	-	-	684121
skeletal muscle	Camelus dromedarius	-	-	684127
skeletal muscle	Mus musculus	-	-	685264
skeletal muscle	Oryctolagus cuniculus	-	-	685334, 685613, 695902, 713466
skeletal muscle	Homo sapiens	-	-	697687
sperm	Bos taurus, Canis lupus familiaris, Homo sapiens, Mus musculus, Oryctolagus cuniculus	-	-	685292
sperm	Homo sapiens	O14556	GAPDS exists in sperms bound to the fibrous sheath of the flagellum	685294
sperm	Rattus norvegicus	Q9ESV6	-	698925
spleen	Mus musculus	-	-	688791
spleen	Oplegnathus fasciatus	B5AAJ5, B5AAJ6	poor expression; strong expression	698019
stem	Arabidopsis thaliana	P25858, Q56WJ4	-	689409
tail	Mus musculus	-	-	688791
tail muscle	Panulirus versicolor	P56649	-	654051
testis	Rattus norvegicus	-	-	676108
trophozoite	Entamoeba histolytica	Q27646	-	686607
tuber	Solanum tuberosum	Q8LK04	-	675137
U-937 cell	Homo sapiens	-	-	654926, 686867, 695602
uterus	Ascaris suum	Q95YJ4	-	656745
vastus lateralis	Homo sapiens	-	-	697687
vegetative	Bacillus cereus	-	-	287974
lung	Mus musculus	-	-	688791
additional information	Caenorhabditis elegans	-	isoenzyme 1 is present in all cells	287928
additional information	Ascaris suum	Q95YJ4	ubiquitously expressed in all stages of the organism	656745
additional information	Rattus norvegicus	-	COS7L cells are used for transfection	674503
additional information	Oplegnathus fasciatus	B5AAJ5, B5AAJ6	mRNA is detected ubiquitously in all tissues examined; mRNA is detected ubiquitously in all tissues examined	698019

LOCALIZATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	GeneOntology No.	LITERATURE	SOURCE
cell surface	Streptococcus pneumoniae	Q97NL1	-	9986	655720
cell surface	Paracoccidioides brasiliensis	Q8X1X3	-	9986	673986
cell surface	Streptococcus pyogenes	P0C0G6	in complex with the streptococcal plasmin receptor, Plr, and streptococcal surface dehydrogenase, SDH	9986	674559
cell surface	Lactobacillus crispatus	-	GAPDH attaches to the cell wall at pH 5 but is released to the medium at pH 8	9986	687380
cell wall	Kluyveromyces marxianus	-	associated with the cell wall	5618	674688
chloroplast	Pinus sylvestris	-	organism contains a cytosolic and a nuclear-encoded, plastid specific enzyme	9507	287922
chloroplast	Marsilea quadrifolia	O65843	-	9507	287956
chloroplast	Pinus sylvestris	-	-	9507	287956
chloroplast	Arabidopsis thaliana, Spinacia oleracea	-	-	9507	673587, 676334
chloroplast	Arabidopsis thaliana	Q84WR0, Q9SAJ6	;	9507	700836
cytoplasm	Homo sapiens	-	-	5737	654849, 672330, 684972
cytoplasm	Streptococcus pneumoniae	Q97NL1	-	5737	655720
cytoplasm	Paracoccidioides brasiliensis	Q8X1X3	-	5737	673986
cytoplasm	Plasmodium falciparum	Q8IKK7	-	5737	677046
cytoplasm	Homo sapiens	O14556	;	5737	685294
cytoplasm	Rattus norvegicus	-	cytoplasmic expression is elevated in apoptosis and proliferation	5737	688949
cytoplasm	Rattus norvegicus	P04797	O-GlcNAcylation at residue T227 interrupts the hydrophobic interfaces formed between the enzyme monomers in its terameric state and allows for nucleic translocation of the cytoplasmic enzyme	5737	696464
cytoplasm	Escherichia coli	-	-	5737	698374
cytosol	Cyanophora paradoxa	-	-	5829	11542
cytosol	Trypanosoma brucei	-	-	5829	287900, 287901
cytosol	Bos taurus	-	-	5829	287911, 685292
cytosol	Pinus sylvestris	-	organism contains a cytosolic and a nuclear-encoded, plastid specific enzyme	5829	287922
cytosol	Sinapis alba	-	-	5829	287923
cytosol	Mus musculus	-	-	5829	287982, 685292
cytosol	Rattus norvegicus	-	-	5829	656072, 657393
cytosol	Arabidopsis thaliana	-	isoform GadC-1	5829	656414
cytosol	Oryctolagus cuniculus	-	-	5829	670846, 673706, 685292, 689409
cytosol	Cavia porcellus	-	-	5829	670846
cytosol	Homo sapiens	-	-	5829	673629, 673706, 676259, 684120, 685025, 685292, 699592
cytosol	Mammalia	-	-	5829	675023
cytosol	Solanum tuberosum	Q8LK04	-	5829	675137
cytosol	Sardina pilchardus	-	-	5829	684121
cytosol	Camelus dromedarius	-	-	5829	684127
cytosol	Canis lupus familiaris	-	-	5829	685292
cytosol	Entamoeba histolytica	Q27646	-	5829	686607
cytosol	Plasmodium brasilianum	B0F3R1	-	5829	687069
cytosol	Plasmodium coatneyi	B0F3R2	-	5829	687069
cytosol	Plasmodium cynomolgi	B0F3R8	-	5829	687069
cytosol	Plasmodium falciparum	B0F3R3	-	5829	687069
cytosol	Plasmodium fragile	B0F3R4	-	5829	687069
cytosol	Plasmodium knowlesi strain H	B0F3R5	-	5829	687069
cytosol	Plasmodium malariae	B0F3R6	-	5829	687069
cytosol	Plasmodium reichenowi	B0F3R7	-	5829	687069
cytosol	Plasmodium vivax	B0F3R9	-	5829	687069
cytosol	Homo sapiens	-	protein complex containing GAPDH and androgen receptor in the cytosol	5829	687584
cytosol	Arabidopsis thaliana	P25858, Q56WJ4	;	5829	689409
cytosol	Saccharomyces cerevisiae, Spinacia oleracea	-	-	5829	689409
cytosol	Homo sapiens, Mesocricetus auratus	-	GAPDH colocalizes with viral RNA-dependentRNA polymerase in cells infected with Japanese encephalitis virus. GAPDH remains relatively constant in the cytosol, while increasing at 12 to 24 hours postinfection and decreasing at 36 hours postinfection in the nuclear fraction of infected cells. There is no direct protein-protein interaction; instead, GAPDH binds to the 3' termini of plus- and minus-strand RNAs of Japanese encephalitis virus. GAPDH binds to the minus-strand more efficiently than to the plus-strand of Japanese encephalitis virus RNAs	5829	699035
cytosol	Arabidopsis thaliana	-	-	5829	700811
extracellular	Streptococcus pyogenes	P0C0G6	the Plr-SDH-GAPDH complex is expressed on the bacterial surface and released from cells	-	674559
extracellular	Escherichia coli	-	secreted GAPDH is located on the bacterial surface and released to the culture medium of enterohemorrhagic and enteropathogenic strains, secreted GAPDH remains associated with colonic Caco-2 epithelial cells after adhesion and binds human plasminogen and fribinogen, non-pathogenic Escherichia coli strains do not secrete GAPDH	-	687114
extracellular	Lactobacillus crispatus	-	GAPDH attaches to the cell wall at pH 5 but is released to the medium at pH 8	-	687380
extracellular	Escherichia coli	-	-	-	698374
flagellum	Rattus norvegicus	-	fibrous sheath of the sperm flagellum	19861	676108
glycosome	Leishmania mexicana	-	-	20015	287986
glyoxysome	Trypanosoma brucei	-	-	9514	287900, 287901
membrane	Homo sapiens	-	isolated from human erytrocyt ghosts	16020	287945, 287950
membrane	Homo sapiens	-	-	16020	654849, 672330, 673629, 673706, 676259
membrane	Rattus norvegicus	-	membrane proteins extracted from rat heart	16020	674503
membrane	Mammalia	-	-	16020	675023
membrane	Schistosoma mansoni	-	intact ex vivo larvae and 5 day old in vitro grown larvae express glyceraldehyde 3-phosphate dehydrogenase on their surface membrane	16020	698033
mitochondrion	Homo sapiens	-	enzyme form E6.8, E8.5 and E9.0	5739	287913
nucleus	Homo sapiens	-	minor amounts	5634	654849
nucleus	Homo sapiens	-	-	5634	672330, 676259, 684972, 685292, 686080, 699592
nucleus	Mammalia	-	-	5634	675023
nucleus	Bos taurus, Canis lupus familiaris, Mus musculus, Oryctolagus cuniculus	-	-	5634	685292
nucleus	Homo sapiens	-	protein complex containing GAPDH and androgen receptor in the nucleus	5634	687584
nucleus	Rattus norvegicus	-	nuclear expression of GAPDH increases in apoptosis	5634	688949
nucleus	Arabidopsis thaliana	P25858, Q56WJ4	; of protoplast, both isoforms GapC1 and GapC2; of protoplast, both isoforms GapC1 and GapC2	5634	689409
nucleus	Rattus norvegicus	P04797	O-GlcNAcylation at residue T227 interrupts the hydrophobic interfaces formed between the enzyme monomers in its terameric state and allows for nucleic translocation of the cytoplasmic enzyme	5634	696464
nucleus	Homo sapiens, Mesocricetus auratus	-	GAPDH colocalizes with viral RNA-dependentRNA polymerase in cells infected with Japanese encephalitis virus. GAPDH remains relatively constant in the cytosol, while increasing at 12 to 24 hours postinfection and decreasing at 36 hours postinfection in the nuclear fraction of infected cells. There is no direct protein-protein interaction; instead, GAPDH binds to the 3' termini of plus- and minus-strand RNAs of Japanese encephalitis virus. GAPDH binds to the minus-strand more efficiently than to the plus-strand of Japanese encephalitis virus RNAs	5634	699035
plasma membrane	Bos taurus	-	the enzyme reversibly interacts with a protease-sensitive plasma membrane-specific protein of rad outer segments	5886	287905
plasma membrane	Homo sapiens	-	-	5886	287910
plasma membrane	Bos taurus	-	neuronal	5886	656594
plastid	Arabidopsis thaliana	-	isoform GadCp-1	9536	656414
plastid	Arabidopsis thaliana	Q84WR0, Q9SAJ6	;	9536	700836
protoplast	Arabidopsis thaliana	P25858, Q56WJ4	-	-	689409
mitochondrion	Rattus norvegicus	-	-	5739	287913
additional information	Homo sapiens	-	the subcellular localization of enzyme form E6.6 is uncertain	-	287913

PDB	SCOP	CATH	ORGANISM
3l0d, download	SCOP (3l0d)	CATH (3l0d)	Bartonella henselae
3hja, download	SCOP (3hja)	CATH (3hja)	Borrelia burgdorferi (strain ATCC 35210 / B31 / CIP 102532 / DSM 4680)
1vsu, download	SCOP (1vsu)	CATH (1vsu)	Cryptosporidium parvum
1vsv, download	SCOP (1vsv)	CATH (1vsv)	Cryptosporidium parvum
3cif, download	SCOP (3cif)	CATH (3cif)	Cryptosporidium parvum
1dc3, download	SCOP (1dc3)	CATH (1dc3)	Escherichia coli (strain K12)
1dc4, download	SCOP (1dc4)	CATH (1dc4)	Escherichia coli (strain K12)
1dc5, download	SCOP (1dc5)	CATH (1dc5)	Escherichia coli (strain K12)
1dc6, download	SCOP (1dc6)	CATH (1dc6)	Escherichia coli (strain K12)
1gad, download	SCOP (1gad)	CATH (1gad)	Escherichia coli (strain K12)
1gae, download	SCOP (1gae)	CATH (1gae)	Escherichia coli (strain K12)
1s7c, download	SCOP (1s7c)	CATH (1s7c)	Escherichia coli (strain K12)
2vyn, download	SCOP (2vyn)	CATH (2vyn)	Escherichia coli (strain K12)
2vyv, download	SCOP (2vyv)	CATH (2vyv)	Escherichia coli (strain K12)
1dbv, download	SCOP (1dbv)	CATH (1dbv)	Geobacillus stearothermophilus
1gd1, download	SCOP (1gd1)	CATH (1gd1)	Geobacillus stearothermophilus
1npt, download	SCOP (1npt)	CATH (1npt)	Geobacillus stearothermophilus
1nq5, download	SCOP (1nq5)	CATH (1nq5)	Geobacillus stearothermophilus
1nqa, download	SCOP (1nqa)	CATH (1nqa)	Geobacillus stearothermophilus
1nqo, download	SCOP (1nqo)	CATH (1nqo)	Geobacillus stearothermophilus
2dbv, download	SCOP (2dbv)	CATH (2dbv)	Geobacillus stearothermophilus
2gd1, download	SCOP (2gd1)	CATH (2gd1)	Geobacillus stearothermophilus
3cmc, download	SCOP (3cmc)	CATH (3cmc)	Geobacillus stearothermophilus
3dbv, download	SCOP (3dbv)	CATH (3dbv)	Geobacillus stearothermophilus
4dbv, download	SCOP (4dbv)	CATH (4dbv)	Geobacillus stearothermophilus
1gpd, download	SCOP (1gpd)	CATH (1gpd)	Homarus americanus
4gpd, download	SCOP (4gpd)	CATH (4gpd)	Homarus americanus
1u8f, download	SCOP (1u8f)	CATH (1u8f)	Homo sapiens
1znq, download	SCOP (1znq)	CATH (1znq)	Homo sapiens
2feh, download	SCOP (2feh)	CATH (2feh)	Homo sapiens
3gpd, download	SCOP (3gpd)	CATH (3gpd)	Homo sapiens
3h9e, download	SCOP (3h9e)	CATH (3h9e)	Homo sapiens
3pfw, download	SCOP (3pfw)	CATH (3pfw)	Homo sapiens
2i5p, download	SCOP (2i5p)	CATH (2i5p)	Kluyveromyces marxianus
1a7k, download	SCOP (1a7k)	CATH (1a7k)	Leishmania mexicana
1gyp, download	SCOP (1gyp)	CATH (1gyp)	Leishmania mexicana
1gyq, download	SCOP (1gyq)	CATH (1gyq)	Leishmania mexicana
1i32, download	SCOP (1i32)	CATH (1i32)	Leishmania mexicana
1i33, download	SCOP (1i33)	CATH (1i33)	Leishmania mexicana
1cf2, download	SCOP (1cf2)	CATH (1cf2)	Methanothermus fervidus
1j0x, download	SCOP (1j0x)	CATH (1j0x)	Oryctolagus cuniculus
3e5r, download	SCOP (3e5r)	CATH (3e5r)	Oryza sativa subsp. japonica
3e6a, download	SCOP (3e6a)	CATH (3e6a)	Oryza sativa subsp. japonica
3v1y, download	SCOP (3v1y)	CATH (3v1y)	Oryza sativa subsp. japonica
1crw, download	SCOP (1crw)	CATH (1crw)	Panulirus versicolor
1dss, download	SCOP (1dss)	CATH (1dss)	Panulirus versicolor
1ihx, download	SCOP (1ihx)	CATH (1ihx)	Panulirus versicolor
1ihy, download	SCOP (1ihy)	CATH (1ihy)	Panulirus versicolor
1szj, download	SCOP (1szj)	CATH (1szj)	Panulirus versicolor
1ywg, download	SCOP (1ywg)	CATH (1ywg)	Plasmodium falciparum
2b4r, download	SCOP (2b4r)	CATH (2b4r)	Plasmodium falciparum
2b4t, download	SCOP (2b4t)	CATH (2b4t)	Plasmodium falciparum
2vyn, download	SCOP (2vyn)	CATH (2vyn)	Rattus norvegicus
2vyv, download	SCOP (2vyv)	CATH (2vyv)	Rattus norvegicus
3pym, download	SCOP (3pym)	CATH (3pym)	Saccharomyces cerevisiae (strain ATCC 204508 / S288c)
3hq4, download	SCOP (3hq4)	CATH (3hq4)	Staphylococcus aureus (strain MRSA252)
3k73, download	SCOP (3k73)	CATH (3k73)	Staphylococcus aureus (strain MRSA252)
3k9q, download	SCOP (3k9q)	CATH (3k9q)	Staphylococcus aureus (strain MRSA252)
3ksd, download	SCOP (3ksd)	CATH (3ksd)	Staphylococcus aureus (strain MRSA252)
3ksz, download	SCOP (3ksz)	CATH (3ksz)	Staphylococcus aureus (strain MRSA252)
3kv3, download	SCOP (3kv3)	CATH (3kv3)	Staphylococcus aureus (strain MRSA252)
3l4s, download	SCOP (3l4s)	CATH (3l4s)	Staphylococcus aureus (strain MRSA252)
3l6o, download	SCOP (3l6o)	CATH (3l6o)	Staphylococcus aureus (strain MRSA252)
3lc1, download	SCOP (3lc1)	CATH (3lc1)	Staphylococcus aureus (strain MRSA252)
3lc2, download	SCOP (3lc2)	CATH (3lc2)	Staphylococcus aureus (strain MRSA252)
3lc7, download	SCOP (3lc7)	CATH (3lc7)	Staphylococcus aureus (strain MRSA252)
3lvf, download	SCOP (3lvf)	CATH (3lvf)	Staphylococcus aureus (strain MRSA252)
1b7g, download	SCOP (1b7g)	CATH (1b7g)	Sulfolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2)
1hdg, download	SCOP (1hdg)	CATH (1hdg)	Thermotoga maritima (strain ATCC 43589 / MSB8 / DSM 3109 / JCM 10099)
1cer, download	SCOP (1cer)	CATH (1cer)	Thermus aquaticus
2g82, download	SCOP (2g82)	CATH (2g82)	Thermus aquaticus
1vc2, download	SCOP (1vc2)	CATH (1vc2)	Thermus thermophilus
3sth, download	SCOP (3sth)	CATH (3sth)	Toxoplasma gondii
2x0n, download	SCOP (2x0n)	CATH (2x0n)	Trypanosoma brucei brucei
1k3t, download	SCOP (1k3t)	CATH (1k3t)	Trypanosoma cruzi
1ml3, download	SCOP (1ml3)	CATH (1ml3)	Trypanosoma cruzi
1qxs, download	SCOP (1qxs)	CATH (1qxs)	Trypanosoma cruzi
3dmt, download	SCOP (3dmt)	CATH (3dmt)	Trypanosoma cruzi
3ids, download	SCOP (3ids)	CATH (3ids)	Trypanosoma cruzi

MOLECULAR WEIGHT	MOLECULAR WEIGHT MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
36000	-	Arabidopsis thaliana	-	monomer, determined by SDS-PAGE	663144
36000	-	Plasmodium falciparum	-	monomer	671063
36000	-	Oryctolagus cuniculus	-	monomer, determined by SDS-PAGE	672402
36000	-	Homo sapiens	-	subunit, SDS-PAGE	684120
36000	-	Escherichia coli	-	SDS-PAGE	687114
36000	-	Homo sapiens, Oryctolagus cuniculus	-	SDS-PAGE, native enzyme	687602
36000	-	Tetrahymena pyriformis	-	subunit, SDS-PAGE	688066
36140	-	Spinacia oleracea	-	theoretical mass of the A subunit	673587
36200	-	Lentinus polychrous	D3K1B6	calculated from amino acid sequence	710790
36600	-	Plasmodium falciparum	Q8IKK7	monomer	677046
37000	-	Homo sapiens	-	-	672330
37000	-	Rattus norvegicus	-	endogenously expressed in COS7L cells, immunoblot	674503
37000	-	Camelus dromedarius	-	SDS-PAGE	684127
37000	-	Entamoeba histolytica	Q27646	ADP-ribosylated enzyme subunit, SDS-PAGE	686607
38000	-	Homo sapiens	-	determined by SDS-PAGE and Western Blot analysis	676259
39000	-	Paracoccidioides brasiliensis	Q8X1X3	determined by SDS-PAGE and Western Blot analysis	673986
39360	-	Spinacia oleracea	-	theoretical mass of the B subunit	673587
40000	-	Homo sapiens, Oryctolagus cuniculus	-	SDS-PAGE	673706
40000	-	Lactobacillus plantarum	-	SDS-PAGE	687344
58000	-	Homo sapiens	O14556	full length subunit	685294
70000	-	Oryctolagus cuniculus	-	SDS-PAGE, treatment of GAPDH with (E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide at 0.01 mM for 10 min at 37�C results in formation of three bands (66, 68, and 76 kDa) corresponding roughly to a dimer of 70000 Da	687602
98000	-	Homo sapiens	-	enzyme form E6.6, gel filtration	287913
120000	-	Homo sapiens	-	enzyme form E8.5, gel filtration	287913
120000	-	Arabidopsis thaliana	-	tetramer, determined by gel filtration	663144
128000	-	Thermus thermophilus	-	equilibrium sedimentation	287968
130000	-	Plasmodium falciparum	-	tetramer, estimated by gel filtration	671063
130000	-	Kluyveromyces marxianus	-	tetramer, determined by gel filtration	674688
131000	-	Caenorhabditis elegans	-	gel filtration	287928
133000	-	Homo sapiens	-	enzyme form E9.0, gel filtration	287913
135000	-	Thermus thermophilus	-	gel filtration	287968
137000	-	Euglena gracilis	-	gel filtration	287973
138600	-	Euglena gracilis	-	sucrose density gradient centrifugation	287973
140000	150000	Trypanosoma brucei	-	sucrose density gradient centrifugation	287901
140000	-	Apis mellifera	-	gel filtration	287971
140500	-	Euglena gracilis	-	equilibrium sedimentation	287973
142000	-	Cyanophora paradoxa	-	gel filtration	11542
142000	-	Bos taurus	-	gel filtration	287911
142000	-	Homo sapiens	-	enzyme form E6.8, gel filtration	287913
142000	-	Homo sapiens	-	equilibrium sedimentation	287932
142000	-	Dissostichus mawsoni	-	low speed equilibrium sedimentation	287978
142000	-	Bos taurus	-	gel filtration	287979
142000	-	Oryctolagus cuniculus	-	-	670846
144000	-	Homo sapiens	-	gel filtration	287950
144000	-	Oryctolagus cuniculus	-	tetramer, calculated	672402
145000	148000	Sus scrofa	-	equilibrium sedimentation, velocity sedimentation	287934
145000	-	Pisum sativum	-	velocity sedimentation	287936
145000	-	Saccharomyces cerevisiae	-	gel filtration	287937
146000	-	Camelus dromedarius	-	non-denaturing PAGE	684127
146800	-	Bacillus coagulans	-	equilibrium sedimentation	287963
147000	-	Glycine max	-	gel filtration	287902
148000	-	Thermotoga maritima	-	equilibrium sedimentation	287906
148000	-	Sus scrofa	-	equilibrium sedimentation	287966
148700	-	Geobacillus stearothermophilus	-	equilibrium sedimentation	287970
150000	-	Spinacia oleracea	-	-	287961
150000	-	Zymomonas mobilis	-	gel filtration	3241
150000	-	Homo sapiens	O14556	N-terminally truncated enzyme after trypsin-treatment, Blue Native PAGE	685294
150000	-	Mus musculus	-	SDS-PAGE	688791
150000	-	Homo sapiens	-	PAGE	695298
152000	-	Hypocrea koningii	-	gel filtration	287908
180000	-	Streptomyces arenae	-	gel filtration, pentalenolactone-sensitive enzyme	287930
180000	-	Spinacia oleracea	-	-	287935
180000	-	Saccharomyces cerevisiae	-	gel filtration	287948
190000	-	Haloarcula vallismortis	-	gel filtration	287907
200000	-	Neisseria meningitidis	-	SDS-PAGE	688953
208000	-	Neisseria meningitidis	-	native PAGE slab gels with varying acrylamide concentrations of 5-10% (w/v) in the absence of SDS	688953
209200	-	Neisseria meningitidis	-	calculated from amino acid sequence	688953
290000	-	Streptomyces arenae	-	gel filtration, pentalenolactone-insensitive enzyme	287930
315000	-	Pseudomonas aeruginosa	-	non-denaturing PAGE	287919
additional information	-	Rattus norvegicus	-	unusually low His content compared to other mammalian muscle acetaldehyde phosphate dehydrogenases	287960
additional information	-	Spinacia oleracea	-	Cys content is higher than that found in analogous enzymes	287961
additional information	-	Geobacillus stearothermophilus	-	the enzyme possesses precise 222 symmetry. Pairs of active sites are linked through a flexible polypeptide loop which probably mediates the structural changes giving rise to cooperative effects. Three additional salt bridges made by each subunit to others would make a major contribution to thermostability of the tetramer	287962
additional information	-	Bacillus cereus	-	high content of beta-structure, 45%	287974
additional information	-	Apis mellifera, Bombus appositus, Bombus nevadensis, Bombus occidentalis, Psithyrus suckleyi	-	comparison of structural properties	287976

SUBUNITS	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
?	Saccharomyces cerevisiae	-	x * 36000, SDS-PAGE	287899
?	Bos taurus	-	x * 38000, SDS-PAGE	287905
?	Zymomonas mobilis	-	x * 41000, SDS-PAGE	287916
?	Pinus sylvestris	-	x * 36000, SDS-PAGE	287922
?	Drosophila melanogaster	-	x * 35500 + x * 37000, SDS-PAGE	287924
?	Mus musculus	-	x * 37000, SDS-PAGE	287958
?	Spinacia oleracea	-	x * 14000 + x * 37000, SDS-PAGE	287961
?	Escherichia coli	-	x * 35000, high speed equilibrium sedimentation after treatment with 5 M guanidine hydrochloride containing 0.01 M dithiothreitol	287977
?	Ascaris suum	Q95YJ4	x * 40000, SDS-PAGE	656745
?	Bacillus megaterium	P23722	x * 35914, calculated from sequence	682151
?	Staphylococcus aureus	Q6GIL8	x * 37600, calculated, x * 37500, SDS-PAGE	695363
?	Antheraea mylitta	-	x * 37600, calculated and SDS-PAGE	695391
?	Oryctolagus cuniculus	-	x * 37000, SDS-PAGE	695902
?	Dunaliella salina	B1PL92	x * 40270, calculated	697508
?	Oplegnathus fasciatus	B5AAJ5, B5AAJ6	x * 35960, calculated; x * 35990, calculated	698019
?	Pseudomonas sp.	A7LHM7	x * 36100, calculated	698639
?	Starmerella bombicola	B3FPE3	x * 35673, calculated	699355
dimer	Homo sapiens	-	1 * 58000 + 1 * 61000, enzyme form E6.6, SDS-PAGE	287913
dimer	Kluyveromyces marxianus	-	the final crystallographic model consists of a dimer	674688
dimer	Entamoeba histolytica	-	x-ray crystallography	687842
heteromer	Arabidopsis thaliana	-	GAPDH-AnBn heteromer of GapA and GapB subunits	676334
heterotetramer	Spinacia oleracea	-	A2B2-GAPDH, light conformation, B subunits are almost identical to A subunits, expect for the presence of a C-terminal extension containing a pair of cysteines, which is the target of TRX regulation	673587
homotetramer	Arabidopsis thaliana	-	-	663144
homotetramer	Plasmodium falciparum	-	-	671063
homotetramer	Homo sapiens	-	-	671066
homotetramer	Arabidopsis thaliana	-	A4-GAPDH	673587
homotetramer	Arabidopsis thaliana, Spinacia oleracea	-	GAPDH-A4, homotetramer GapA subunits	676334
homotetramer	Sardina pilchardus	-	4 * 37000, denaturing SDS-PAGE; 4 * 38500, non-denaturing PAGE	684121
homotetramer	Camelus dromedarius	-	4 * 36500, non-denaturing PAGE; 4 * 37000, SDS-PAGE	684127
homotetramer	Homo sapiens	O14556	4 * 40000, N-terminally truncated enzyme after trypsin treatment, SDS-PAGE	685294
homotetramer	Entamoeba histolytica	Q27646	-	686053
homotetramer	Mus musculus	-	4 * 37000, SDS-PAGE	688791
homotetramer	Neisseria meningitidis	-	4 * 50000, SDS-PAGE; 4 * 51000, native PAGE slab gels with varying acrylamide concentrations of 5-10% (w/v) in the absence of SDS	688953
homotetramer	Staphylococcus aureus	Q6GIL8	x-ray crystallography	710757, 712766
homotetramer	Cryptosporidium parvum	-	x-ray crystallography	711617
homotetramer	Oryctolagus cuniculus	-	-	713466
multimer	Spinacia oleracea	-	A8B8-GAPDH, dark conformation, B subunits are almost identical to A subunits, expect for the presence of a C-terminal extension containing a pair of cysteines, which is the target of TRX regulation	673587
octamer	Streptomyces arenae	-	8 * 43000, pentalenolactone-insensitive enzyme, gel filtration	287930
tetramer	Cyanophora paradoxa	-	4 * 36000, SDS-PAGE	11542
tetramer	Trypanosoma brucei	-	4 * 33500, cytosolic enzyme, SDS-PAGE; 4 * 38000, glyoxysomal enzyme, SDS-PAGE	287901
tetramer	Glycine max	-	4 * 37000, SDS-PAGE	287902
tetramer	Thermotoga maritima	-	4 * 37000, SDS-PAGE	287906
tetramer	Haloarcula vallismortis	-	4 * 40000, SDS-PAGE	287907
tetramer	Hypocrea koningii	-	4 * 38000, GAPDH I and GAPDH II, SDS-PAGE	287908
tetramer	Bos taurus	-	4 * 35000, SDS-PAGE	287911
tetramer	Homo sapiens	-	4 * 29500, enzyme form E8.5, SDS-PAGE; 4 * 33000, enzyme form E9.5, SDS-PAGE; x * 36000 + x * 38000, enzyme form E6.8, SDS-PAGE	287913
tetramer	Caenorhabditis elegans	-	4 * 38500, SDS-PAGE	287928
tetramer	Streptomyces arenae	-	4 * 43000, pentalenolactone-sensitive enzyme, gel filtration	287930
tetramer	Oryctolagus cuniculus	-	-	287934, 287946, 670846, 672402
tetramer	Sus scrofa	-	4 * 38000, glyoxysomal enzyme, SDS-PAGE	287934
tetramer	Pisum sativum	-	4 * 36000-37000, SDS-PAGE	287936
tetramer	Saccharomyces cerevisiae	-	4 * 35000, SDS-PAGE	287937
tetramer	Thermus thermophilus	-	4 * 33500, SDS-PAGE	287938
tetramer	Geobacillus stearothermophilus	-	4 * 36000, SDS-PAGE	287946, 287970
tetramer	Thermus aquaticus	-	4 * 36000, SDS-PAGE	287946
tetramer	Saccharomyces cerevisiae	-	4 * 37000, SDS-PAGE	287948
tetramer	Homo sapiens	-	4 * 36000, SDS-PAGE	287950, 695298
tetramer	Bacillus coagulans	-	4 * 36000, SDS-PAGE	287963
tetramer	Sus scrofa	-	4 * 38000, SDS-PAGE	287966
tetramer	Thermus thermophilus	-	4 * 33700, SDS-PAGE	287968
tetramer	Euglena gracilis	-	4 * 37000, SDS-PAGE	287973
tetramer	Bacillus cereus	-	4 * 36000, SDS-PAGE	287974
tetramer	Zymomonas mobilis	-	4 * 40000-42000, SDS-PAGE	3241
tetramer	Pleurodeles waltl	Q8AXB5	4 * 37000, SDS-PAGE	655270
tetramer	Xenopus laevis	-	4 * 35000, SDS-PAGE	655270
tetramer	Cavia porcellus	-	-	670846
tetramer	Geobacillus stearothermophilus	-	-	672306
tetramer	Homo sapiens	-	-	673629, 688250
tetramer	Kluyveromyces marxianus	-	determined by gel filtration	674688
tetramer	Plasmodium falciparum	Q8IKK7	-	677046
tetramer	Oryctolagus cuniculus	-	sedimentation analysis	685613
monomer	Homo sapiens, Oryctolagus cuniculus	-	1 * 36000, SDS-PAGE	687602
additional information	Saccharomyces cerevisiae	-	subunit interactions are involved in regulation of activity	287931
additional information	Saccharomyces cerevisiae	-	immobilized enzyme can exist as a trimer	287943
additional information	Oryctolagus cuniculus	-	enzyme exists as an equilibrium mixture of different oligomeric states. Rapid equilibrium between monomer - dimer - tetramer, the tetramer is inactive	287953
additional information	Geobacillus stearothermophilus	-	dimers generated from the tetrameric enzyme are inactive but exhibit cooperativity in NAD+ binding	287985
additional information	Arabidopsis thaliana	P25858, Q56WJ4	enzyme can bind top a partial gene sequence of NADP-dependent malated dehydrogenase EC 1.2.1.37; enzyme can bind top a partial gene sequence of NADP-dependent malated dehydrogenase EC 1.2.1.37	689409
additional information	Homo sapiens	-	recombinant glyceraldehyde 3-phosphate dehydrogenase binds to hepatitis B virus regulatory element RNA in vitro and inhibits hepatitis B virus regulatory element function. Overexpression of glyceraldehyde 3-phosphate dehydrogenase depresses the expression of hepatitis B virus antigen	695939
additional information	Oryctolagus cuniculus	-	non-native forms of GAPDH obtained by cold denaturation, oxidation of the enzyme, or its unfolding in guanidine hydrochloride efficiently bind to soluble amyloid-beta peptide (1-42) yielding a stable complex. Native tetrameric GAPDH does not interact with soluble amyloid-beta peptide (1-42), neither non-native forms of GAPDH interact with aggregated amyloid-beta peptide (1-42)	696421
additional information	Streptococcus oralis	-	interaction of GAPDH with Porphyromonas gingivalis major fimbrae plays an important role in Porphyromonas gingivalis colonization. Amino acid residues 166 to 183 of Streptococcus oralis GAPDH exhibit the strongest binding activity toward rFimA, and the synthetic peptide corresponding to amino acid residues 166 to 183 of GAPDH, peptide DNFGVVEGLMTTIHAYTG inhibits Streptococcus oralis-Porphyromonas gingivalis biofilm formation in a dose-dependent manner. The peptide inhibits interbacterial biofilm formation by several oral streptococci and Porphyromonas gingivalis strains with different types of FimA	698256
additional information	Homo sapiens	-	recombinant GAPDH binds directly with high affinity to a single-stranded oligonucleotide comprising three telomeric DNA repeats. Nucleotides T1, G5, and G6 of the TTAGGG repeat are essential for binding.The stoichiometry of the interaction is 2:1 DNA:GAPDH, and GAPDH appears to form a high-molecular-weight complex when bound to the oligonucleotide	699592

POSTTRANSLATIONAL MODIFICATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
glutathionylation	Arabidopsis thaliana	-	-	673587
phosphorylation	Homo sapiens	-	-	688661
side-chain modification	Homo sapiens	-	dimethylation, deamidation, and methionine oxidation	688661
glycoprotein	Kluyveromyces marxianus	-	N-glycosylated	674688
ribosylation	Entamoeba histolytica	Q27646	mono-ADP-ribosylation does not affect GADPH activity	686607
additional information	Escherichia coli	-	GAPDH of enterohemorrhagic and enteropathogenic Escherichia coli-strains is ADP-ribosylated either in the cytoplasm or in the extracellular medium. GAPDH catalyzes its own modification involving residue C149 at the active site, reaction requires NAD+	698374
additional information	Homo sapiens	-	4-hydroxy-2-nonenal-modified GAPDH is degraded by cathepsin G	686867
phosphoprotein	Mammalia	-	suggested for enzymatic mechanismn which modifiy GAPDHc to GAPDHn	675023
additional information	Oryctolagus cuniculus	-	posttranslational modification of the enzyme with NADH is stimulated by thiols, possibly through superoxide, and is independent of NO	287965
proteolytic modification	Pinus sylvestris	-	the precursor has a MW of 40000 Da, the processed polypeptide has a MW of 36000 Da	287922
additional information	Rattus norvegicus	P04797	O-GlcNAcylation at residue T227 interrupts the hydrophobic interfaces formed between the enzyme monomers in its terameric state and allows for nucleic translocation of the cytoplasmic enzyme	696464
phosphoprotein	Rattus norvegicus	-	phosphorylated by protein kinase Ciota /lambda	656072
additional information	Tetrahymena pyriformis	-	posttranslational modification of the enzyme by nitrogen oxide	688066

Crystallization/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
resolution to 2.2 A. space group I222	Antheraea mylitta	-	695391
-	Apis mellifera	-	287971
-	Bacillus coagulans	-	287963
crystalline enzyme does not contain NAD+	Bos taurus	-	287979
GAPDH in the apo and holo (enzyme in complex with NAD+) state, hanging drop vapor diffusion method, using 16-20% (w/v) PEG 3000 and 0.2 M NaCl in 0.1 M HEPES, pH 7.5	Cryptosporidium parvum	-	711617
structure in absence and presence of NAD+ and structure of the ternary enzyme-cofoactor-substrate complex to 2.0 A resolution. The active site can accomodate the substrate in multiple conformations at multiple locations during the initial encounter. the C-3 phosphate group clearly prefers the so-called new phosphate site for initial binding	Cryptosporidium parvum	-	697008
GADPH in complex wit NAD+, computational modelling	Entamoeba histolytica	-	687842
-	Escherichia coli	-	287983
after addition of NAD+ to the apoprotein	Escherichia coli	-	287977
structure determination by formation of soluble recombinant rat sperm glyceraldehyde-3-phosphate dehydrogenase as a heterotetramer with the Escherichia coli glyceraldehyde-3-phosphate dehydrogenase in a ratio of 1:3. Glyceraldehyde 3-phosphate binds in the Ps pocket in the active site of the sperm enzyme subunit in the presence of NAD	Escherichia coli	P0A9B2	698925
-	Geobacillus stearothermophilus	-	287909, 287962, 287970
hanging-drop vapor-diffusion method, enzyme in complex with NAD+ and D-glyceraldehyde 3-phosphate. C149A GAPDH and C149S GAPDH ternary complexes are obtained by soaking the crystals of the corresponding binary complexes (enzyme -NAD+) in a solution containing D-glyceraldehyde 3-phosphate	Geobacillus stearothermophilus	-	656119
holoenzyme and apoenzyme	Geobacillus stearothermophilus	-	287946
structure at 1.8 A resolution	Geobacillus stearothermophilus	-	287917
-	Homo sapiens	-	287932
a high-resolution structure of 1.75 A is reported	Homo sapiens	-	671070
the structure is presented at 2.5 A resolution	Homo sapiens	-	671066
diffraction data to 2.3 A resolution are collected	Kluyveromyces marxianus	-	674688
-	Leishmania mexicana	-	287986
-	Oryctolagus cuniculus	-	287969
sitting-drop vapour-diffusion method, crystallized in space group P2(1)2(1)2(1) with a tetramer in the asymmetric unit, crystals solved at 2.4 A	Oryctolagus cuniculus	-	654080
-	Palinurus vulgaris	-	287951
enzyme carrying a fluorescent NAD+ derivative at 2.7 A resolution	Panulirus versicolor	-	287987
structures of D-glyceraldehyde-3-phosphate dehydrogenase complexed with coenzyme analogues ADPribose and thio-NAD+. The crystals of both GAPDH complexes belong to the same space group C2 as holeenzyme or apoenzyme, but with different unit-cell parameters	Panulirus versicolor	P56649	654051
crystals of tag-free protein diffracts X-rays to 2.6 A resolution, of the His-tagged protein to 4.0 A	Plasmodium falciparum	-	671063
the crystal structure is solved at 2.25 A resolution	Plasmodium falciparum	Q8IKK7	677046
structure determination by formation of soluble recombinant rat sperm glyceraldehyde-3-phosphate dehydrogenase as a heterotetramer with the Escherichia coli glyceraldehyde-3-phosphate dehydrogenase in a ratio of 1:3. Refinement to 2.2 A for the holo enzyme, to 2.4 A for the complex with glyceraldehyde 3-phosphate. Glyceraldehyde 3-phosphate binds in the Ps pocket in the active site of the sperm enzyme subunit in the presence of NAD	Rattus norvegicus	Q9ESV6	698925
-	Saccharomyces cerevisiae	-	287975
hanging drop vapor diffusion method, using 0.1 M Tris-HCl pH 8.2, 28% (w/v) PEG 3350, at 25�C	Staphylococcus aureus	-	710757
to 2.0 A resolution. Space group P21	Staphylococcus aureus	Q6GIL8	695363
wild type holo and apoenzymes and mutant enzymes C151S, C151G and C151S/H178N in complex with NAD+ and D-glyceraldehyde 3-phosphate, hanging drop vapor diffusion method, the wild-type holoenzyme and apoenzyme are crystallized from 0.1 M Tris-HCl, pH 8.5, and 20% (w/v) PEG 4000 at 4�C and from 0.1 M Tris-HCl, pH 8.2, and 32% (w/v) PEG 3350 at 25�C, respectively. Crystals of active-site mutants in complex with NAD+ (C151S, C151G, and C151S/H178N) grow at 25�C from 0.1 M Tris-HCl, pH 8.5, and 28% (w/v) PEG 4000, from 0.1 M Tris-HCl, pH 8.2, and 30% (w/v) PEG 4000, and from 0.1 M Tris-HCl, pH 8.5, and 30% (w/v) PEG 4000, respectively, while mutant H178N in complex with NAD+ crystallizes from 0.1 M Tris-HCl, pH 8.2, and 30% (w/v) PEG 4000 at 4�C	Staphylococcus aureus	-	712766
-	Sus scrofa	-	287934, 287966
-	Thermotoga maritima	-	287906
-	Thermus thermophilus	-	287938, 287968
hanging drop vapour diffusion method, enzyme in complex with the covalently bound GAP analogue, 3-(p-nitrophenoxycarboxyl)-3-ethylene propyl dihydroxyphosphonate, at an improved resolution of 2.0-2.5 A	Trypanosoma cruzi	-	654711

pH STABILITY	pH STABILITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
4.5	11	Saccharomyces cerevisiae	-	rapid inactivation below pH 4.5 and above pH 11.0	287937
5.6	-	Lactobacillus crispatus	-	GAPDH interacts with lipoteichoic acids at acidic pH values but not at pH values higher than 5.6	687380
8	-	Glycine max	-	very labile, especially above pH 8.0	287920
8.5	-	Saccharomyces cerevisiae	-	27�C, half-life: 2 days	287937

TEMPERATURE STABILITY	TEMPERATURE STABILITY MAXIMUM	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
15	60	Sardina pilchardus	-	pre-incubation for 10 min at temperatures varying between 15�C and 30�C does not irreversibly affect the enzyme activity, thermal inactivation occurs above 35�C and results in total activity loss at 60�C	684121
15	70	Camelus dromedarius	-	pre-incubation for 10 min at temperatures varying between 20 and 32�C does not irreversibly affect the enzyme activity, thermal inactivation occurs above 35�C and results in total activity loss at 70�C	684127
15	-	Pseudomonas aeruginosa	-	5 min, stable	287919
27	-	Saccharomyces cerevisiae	-	pH 8.5, half-life: 2 days	287937
35	-	Dissostichus mawsoni	-	30 min, stable up to	287978
43	-	Rattus norvegicus	-	30 min, 50% inactivation, irreversible	287929
45	-	Oryctolagus cuniculus	-	GAPDH is inactivated at 45�C, alpha-crystallin accelerates the thermal inactivation of GAPDH at 45�C and reduces thermostability of the enzyme, while GroEL does not affect thermal inactivation and denaturation of GAPDH	713466
50	-	Saccharomyces cerevisiae	-	pH 7.5, half-life: 3 h	287937
50	-	Bacillus cereus	-	pH 7.5, 60 min, stable	287974
55	-	Pseudomonas aeruginosa	-	5 min, complete loss of activity	287919
55	-	Bacillus coagulans	-	5 min, 96-98% loss of activity. No inactivation if the ionic strength is increased to 1.8 by addition of NaCl or (NH4)2SO4	287963
55	-	Dissostichus mawsoni	-	30 min, 50% loss of activity	287978
58	-	Oryctolagus cuniculus	-	pH 7.0, 20 min, 50% inactivation	287946
60	70	Geobacillus stearothermophilus	-	retains structural integrity and enzymatic activity up to	287962
60	-	Bacillus cereus	-	pH 7.5, 30 min, about 40% loss of activity	287974
61.6	-	Oryctolagus cuniculus	-	thermal unfolding of GAPDH is characterized by sharp thermal transition with a maximum at 61.6�C, GAPDH stability is diminished in the presence of alpha-crystallin (0.4 mg/ml)	685613
62	-	Geobacillus stearothermophilus	-	maximum of thermal transition peak of tetrameric form of mutant apoenzyme N313T	654653
64.8	-	Geobacillus stearothermophilus	-	maximum of thermal transition peak of dimeric mutant apoenzyme T34Q/T39S/L43Q; maximum of thermal transition peak of tetrameric mutant apoenzyme T34Q/T39S/L43Q	654653
65.4	-	Geobacillus stearothermophilus	-	maximum of thermal transition peak of dimeric mutant apoenzyme Y46G/S48G	654653
65.5	-	Geobacillus stearothermophilus	-	maximum of thermal transition peak of dimeric mutant apoenzyme Y46G/R52G	654653
66	-	Oryctolagus cuniculus	-	melting temperature at 66�C	686481
68.4	-	Geobacillus stearothermophilus	-	maximum of thermal transition peak of tetrameric mutant apoenzyme Y283V	654653
70	-	Bacillus cereus	-	loss of activity within a few min	287974
70.3	-	Geobacillus stearothermophilus	-	maximum of thermal transition peak of tetrameric mutant apoenzyme D283G	654653
70.7	-	Geobacillus stearothermophilus	-	maximum of thermal transition peak of tetrameric mutant apoenzyme W310F	654653
71	-	Geobacillus stearothermophilus	-	maximum of thermal transition peak of dimeric mutant apoenzyme D282G	654653
73.5	-	Geobacillus stearothermophilus	-	maximum of thermal transition peak of dimeric mutant apoenzyme Y283V	654653
75	-	Geobacillus stearothermophilus	-	pH 7.0, 20 min, 50% inactivation	287946
78.5	-	Geobacillus stearothermophilus	-	maximum of thermal transition peak of tetrameric form of wild-type apoenzyme	654653
80	102	Thermotoga maritima	-	the enzyme is fully active at temperatures near 80�C but has very low activity at room temperature, the melting temperature is at 102�C	686481
85	-	Thermus thermophilus	-	pH 8.5, protein concentration 2.1 mg/ml, 10 min, stable	287968
90	-	Thermus thermophilus	-	slow inactivation	287938
90	-	Thermus thermophilus	-	pH 8.5, protein concentration 2.1 mg/ml, 10 min, about 20% loss of acticity	287968
90	-	Geobacillus stearothermophilus	-	10 min, about 10% inactivation	287970
95	-	Thermotoga maritima	-	t1/2 at pH 5.0: 25 min, t1/2 at pH 6.0: more than 120 min, t1/2 at pH 7.5: 50 min	287906
95	-	Thermus thermophilus	-	pH 8.5, protein concentration 2.1 mg/ml, 10 min, about 85% loss of acticity	287968
100	-	Thermotoga maritima	-	t1/2: above 120 min at pH 6	287906
100	-	Thermus aquaticus	-	pH 7.0, 20 min, 50% inactivation	287946
109	-	Thermotoga maritima	-	transition temperature	287906
additional information	-	Thermotoga maritima	-	incubation at 70-85�C results in an apparent activation	287906
additional information	-	Haloarcula vallismortis	-	temperature of 50% inactivation increases by 15�C, when KCl concentration is increased from 1 M to 4 M or when 1 M KCl is replaced by 1 M (NH4)2SO4	287907
additional information	-	Geobacillus stearothermophilus	-	three salt bridges made by each subunit to others would make a major contribution to thermostability of the tetramer	287962

GENERAL STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
8 M urea, 30�C, 0.05 M phosphate buffer, 50% loss of activity after 1 min	Bacillus coagulans	-	287963
5.0 M guanidine-HCl, rapid and irreversible inactivation at 30�C	Geobacillus stearothermophilus	-	287970
8.0 M urea, unusually resistant at 30�C, 40�C and 50�C. Rapid inactivation at 55�C and at 60�C	Geobacillus stearothermophilus	-	287970
very stable in 4 M KCl. Inactivated within 12 h in 0.5 M KCl, 50 mM Tris-HCl, pH 8.0, at 25�C	Haloarcula vallismortis	-	287907
acetylleucine chloromethyl ketone binds to GAPDH to modulate the conformation of the enzyme, the modified enzyme is susceptible to chymotrypsin-like protease activity, cleavage at TRp195-Arg196	Homo sapiens	-	654926
binding to erythrocyte membranes stabilizes the enzyme at 4�C	Homo sapiens	-	287950
alpha-crystallin accelerates the thermal inactivation of GAPDH, while GroEL does not affect thermal inactivation and denaturation of GAPDH	Oryctolagus cuniculus	-	713466
freezing in liquid nitrogen results in partial loss of activity	Oryctolagus cuniculus	-	287933
5 M, 0�C, 1 h, complete loss of activity, 5 mM NAD+ protects	Sus scrofa	-	287966
1% SDS, fully active	Thermus thermophilus	-	287938
8 M urea, 40% inactivation	Thermus thermophilus	-	287938, 287968
repeated freezing and thawing brings about 10-20% inactivation	Thermus thermophilus	-	287968
rapid loss of activity without protection by a thiol	Zymomonas mobilis	-	3241

ORGANIC SOLVENT	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
No entries in this field

OXIDATION STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
stable after reactivation and desalting for at least 2 h when incubated in the presence of 20 mM dithiothreitol, but less stable when both, NAD+ and the reductant, are omitted in the pre-incubation assays, under this condition the enzymes seem to be very sensitive towards traces of oxygen in the media, but upon addition of 20 mM dithiothritol after 1 h, the full activity is restored	Arabidopsis thaliana	P25858, Q56WJ4	689409
stable after reactivation and desalting for at least 2 h when incubated in the presence of 20 mM dithiothreitol, but less stable when both, NAD+ and the reductant, are omitted in the pre-incubation assays, under this condition the enzymes seem to be very sensitive towards traces of oxygen in the media, but upon addition of 20 mM dithiothritol after 1 h, the full activity is restored	Oryctolagus cuniculus, Saccharomyces cerevisiae, Spinacia oleracea	-	689409
the purified enzyme remains mostly unmodified or without any sign of degradation after exposure to NO or H2O2	Tetrahymena pyriformis	-	688066

STORAGE STABILITY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
2�C, stable for several months	Apis mellifera	-	287971
4�C, in 67% saturated ammonium sulfate solution containing EDTA and dithiothreitol, stable for months	Geobacillus stearothermophilus	-	287970
-20�C, 50 mM potassium phosphate buffer, pH 7.4, stable for at least 2 weeks	Glycine max	-	287902
at -20�C storage after dialysis following the first (NH4)2SO4 fractionation step during purification, normal muscle and leukocyte enzyme is stable. Enzyme from chronic myeloid leukemia patients and sarcoma tissue loses about 80% of activity.	Homo sapiens	-	696174
-20�C	Lactococcus lactis	-	712937
4�C, crystalline suspension in 67% saturated ammonium sulfate containing EDTA, KCN, and dithioerythritol is stable for many months	Oryctolagus cuniculus	-	287969
5�C, stable for several months	Oryctolagus cuniculus	-	287933
0-4�C, no substantial loss of activity after several months	Pisum sativum	-	287936
-80�C, HEPES buffer, pH 7.25, 500 mM NaCl, 5% glycerol, 2 mM DTT, 0.025% sodium azide	Plasmodium falciparum	Q8IKK7	677046
4�C, 1 mM EDTA, 75% glycerol, stable	Pseudomonas aeruginosa	-	287919
-20�C, 30% of the original activity remains after 6 months	Spinacia oleracea	-	287935
4�C, pentalenolactone-insensitive enzyme stable up to 6 months. Pentalenolactone-sensitive enzyme loses activity with a half-life of 7 days	Streptomyces arenae	-	287930
-20�C, stable for at least 2 years	Thermus thermophilus	-	287938, 287968
-80�C, triethanolamine (100 mM, pH 7.5), containing 1.0 mM beta-mercaptoethanol, 1.0 mM EDTA, 30 mM sodium arsenate heptahydrate, 1.0 mM phenylmethylsulfonyl fluoride, 50 mM NAD+, 1.0 mM pepstatin, 1.0 mM leupeptin, 0.5 M KCl, and glycerol 10% (v/v), 8 months, 1% loss of activity	Trypanosoma cruzi	-	684427
25�C, free enzyme in Tris-HCl (50 mM, pH 8.6), containing 1.0 mM beta-mercaptoethanol, 30 mM sodium arsenate heptahydrate, and 1.0 mM EDTA, 99% of the enzymatic activity is retained after 24 h and 57% after 120 h	Trypanosoma cruzi	-	684427
4�C, free enzyme in buffer 8, 55% of the enzymatic activity is retained after dialysis and after 48 h the calculated activity has fallen to 7%	Trypanosoma cruzi	-	684427
4�C, free enzyme in Tris-HCl (50 mM, pH 8.6), containing 1.0 mM beta-mercaptoethanol, 30 mM sodium arsenate heptahydrate, and 1.0 mM EDTA, 55% of the enzymatic activity is retained after dialysis and after 48 h the calculated activity has fallen to 25%	Trypanosoma cruzi	-	684427

Purification/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
-	Apis mellifera	-	287971
-	Arabidopsis thaliana	-	673587
ammonium sulfate precipitation and Q-Sepharose column chromatography	Arabidopsis thaliana	P25858, Q56WJ4	689409
purification protocol comprises two anion-exchange chromatographic steps and removing of the His tag	Arabidopsis thaliana	-	663144
histidine fusion protein in Escherichia coli TOP 10 strain	Ascaris suum	Q95YJ4	656745
-	Bacillus cereus	-	287974
-	Bacillus coagulans	-	287963
active-site CNBr peptide	Bacillus coagulans	-	287944
-	Bos taurus	-	287905, 287979
hydrophobic chromatography on immobilized colchicine	Bos taurus	-	287911
isoenzyme 1 and 2	Caenorhabditis elegans	-	287928
ammonium sulfate fractionation and Blue Sepharose column chromatography	Camelus dromedarius	-	684127
Ni-NTA column chromatography, SP Sepharose column chromatography, and Superdex 200 gel filtration	Cryptosporidium parvum	-	711617
-	Cyanophora paradoxa	-	11542
-	Dissostichus mawsoni	-	287978
-	Drosophila melanogaster	-	287924
-	Escherichia coli	-	287983
glutathione Sepharose 4B resin column chromatography	Escherichia coli	-	687114
-	Euglena gracilis	-	287973
-	Geobacillus stearothermophilus	-	287946, 287947, 287970
mutant enzyme C149selenocysteine	Geobacillus stearothermophilus	-	287984
using gel filtration on a AcA-34 column, and a Q-Sepharose and phenyl-Sepharose column	Geobacillus stearothermophilus	-	672306
-	Glycine max	-	287902
-	Haloarcula vallismortis	-	287907
-	Homo sapiens	-	287932, 287950, 654926
4 enzyme forms: E6.6, E6.8, E8.5 and E9.0	Homo sapiens	-	287913
analytical centrifugation	Homo sapiens	-	687584
by affinity chromatography on a Hi-Trap chelating column charged with nickel sulfate, the His tag is cleaved and the enzyme is purified by an additional gel filtration step	Homo sapiens	-	671066
DEAE-Sepharose column chromatography, ammonium sulfate precipitation, phenyl-Sepharose 6 column chromatography, and Superose 6 gel filtration	Homo sapiens	-	686867
from normal leukocytes of healthy subjects, leukocytes of chronic myeloid leukemia patients and from sarcoma tissue	Homo sapiens	-	696174
GST-fusion proteins are expressed in Escherichia coli and subsequently purified by absorption to glutathione-Sepharose beads, GST is seperated by digestion with thrombin protease	Homo sapiens	-	676259
Ni-NTA agarose resin chromatography and Hi-Load 16/60 Superdex gel filtration	Homo sapiens	-	687602
Ni2+-NTA agarose resin chromatography	Homo sapiens	-	684972
phenyl Sepharose column chromatography	Homo sapiens	O14556	685294
red blood cell cytosol is prepared by hypotonic shock or freeze-thawing cycles, membranes are prepared by hypotonic lysis	Homo sapiens	-	673629
use of immunoaffinity column chromatography	Homo sapiens	-	695298
using Ni-NTA resin and a HiTrap Blue column, the His tag is removed	Homo sapiens	-	671070
using a nickel-nitrilo-triacetic acid-agarose column	Kluyveromyces marxianus	-	674688
NAD affinity chromatography	Lactobacillus crispatus	-	687380
DEAE-Toyopearl column chromatography and hydroxyapatite column chromatography	Lactobacillus plantarum	-	687344
-	Lactococcus lactis	-	712937
use of glyceraldehyde-3-phosphate dehydrogenase-depleted human erythrocyte ghosts as specific high affinity adsorbents for the purification	Mus musculus	-	287958
ammonium sulfate precipitation, DEAE-cellulose DE-52 column chromatography, and Cibacron Blue Sepharose column chromatography	Neisseria meningitidis	-	688953
-	Oryctolagus cuniculus	-	287969
affinity column chromatography and Sephadex G-100 gel filtration	Oryctolagus cuniculus	-	685613
ammonium sulfate precipitation and Q-Sepharose column chromatography	Oryctolagus cuniculus	-	689409
GAPDH is subjected to gel filtration on a Sephadex G-100 column	Oryctolagus cuniculus	-	672402
large-scale purification	Oryctolagus cuniculus	-	287972
Ni-NTA agarose resin chromatography and Hi-Load 16/60 Superdex gel filtration	Oryctolagus cuniculus	-	687602
purified on a Ni-nitrilotriacetic acid resin column	Paracoccidioides brasiliensis	Q8X1X3	673986
-	Pisum sativum	-	287936
purified on Superflow NTA nickel resin and gel chromatography, Superdex 75 26/60	Plasmodium falciparum	Q8IKK7	677046
the filtered supernatant of the centrifuged cell lysate is applied onto 3 ml Talon resin, the His-tag is removed, further purification by gel filtration	Plasmodium falciparum	-	671063
-	Pleurodeles waltl	Q8AXB5	655270
radiommunoprecipitation with protein agarose A/G bead	Rattus norvegicus	-	686305
-	Saccharomyces cerevisiae	-	287937, 287975
ammonium sulfate precipitation and Q-Sepharose column chromatography	Saccharomyces cerevisiae	-	689409
high-affinity cAMP-binding protein I and II	Saccharomyces cerevisiae	-	287948
isoenzyme TLAb-1 and TLAb-2	Saccharomyces cerevisiae	-	287899
ammonium sulfate fractionation and Blue Sepharose column chromatography	Sardina pilchardus	-	684121
-	Spinacia oleracea	-	287935, 287961
ammonium sulfate precipitation and Q-Sepharose column chromatography	Spinacia oleracea	-	689409
native GAPDH is purified from spinach chloroplasts	Spinacia oleracea	-	673587
Ni-Sepharose column chromatography and Superdex 75 gel filtration	Staphylococcus aureus	-	710757
recombinant protein is purified using glutathione-Sepharose 4B and PreScission protease	Streptococcus pyogenes	P0C0G6	674559
-	Streptomyces arenae	-	287930
-	Sus scrofa	-	287934, 287966, 3246
ammonium sulfate precipitation and DEAE-cellulose DE-52 column chromatography	Tetrahymena pyriformis	-	688066
-	Thermotoga maritima	-	287906
-	Thermus aquaticus	-	287946
-	Thermus thermophilus	-	287938, 287968
cytosolic isoenzyme	Trypanosoma brucei	-	287900
-	Trypanosoma cruzi	-	684427
-	Vigna radiata var. radiata	-	287949
-	Xenopus laevis	-	655270

Cloned/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
expression in Escherichia coli	Antheraea mylitta	-	695391
expressed in Escherichia coli BL21(DE3)pLysS cells	Arabidopsis thaliana	P25858, Q56WJ4	689409
for expression in Escherichia coli BL21DE	Arabidopsis thaliana	-	673587
into the pET28a vector for expression in Escherichia coli BL21DE3 cells, amplificatied in Escherichia coli HB101 cells	Arabidopsis thaliana	-	663144
expressed as a histidine fusion protein in Escherichia coli TOP 10 strain	Ascaris suum	Q95YJ4	656745
expression in Escherichia coli	Bacillus megaterium	P23722	679982
determination of complete nucleotide sequence of the coding as well as the noncoding flanking regions of the gene	Caenorhabditis elegans	-	287918
expressed in Escherichia coli	Cryptosporidium parvum	-	711617
expression in Escherichia coli	Cryptosporidium parvum	-	697008
genome contains two unlinked genes for D-glyceraldehyde 3-phosphate dehydrogenase: one of them encodes a protein of 37000 Da and the other a protein of 35500 Da	Drosophila melanogaster	-	287924
expression in Escherichia coli	Dunaliella salina	B1PL92	697508
-	Emericella nidulans	-	287915
-	Escherichia coli	-	698374
expressed in Escherichia coli strain BL21(DE3)	Escherichia coli	-	687114
cloned into the pBluescript vector for expression in Escherichia coli GM-109 cells	Geobacillus stearothermophilus	-	672306
expression of wild-type and mutant enzyme W84F in Escherichia coli	Geobacillus stearothermophilus	-	287947
-	Homo sapiens	-	287925
expressed in Escherichia coli	Homo sapiens	-	684972
expressed in Escherichia coli strainW3CG and in HeLa cells	Homo sapiens	-	687602
expressed in Escherichia coli TRG8 cells	Homo sapiens	-	686080
expressed in HEK-293T cells	Homo sapiens	-	688661
expressed in MCF-7 cells	Homo sapiens	-	684120
expressed in prostate cancer cell lines PC-3 and LNCaP	Homo sapiens	-	687584
expression in 293Tcells and HepG2.2.15 cells	Homo sapiens	-	695939
expression in COS-7 cell	Homo sapiens	-	698989
expression in Escherichia coli	Homo sapiens	-	699592
into a pGEX-5X-3 and a pGFP-C2 vector	Homo sapiens	-	676259
subcloned into a pET14b vector for expression in Escherichia coli BL21DE3 cells	Homo sapiens	-	671070
subcloned into the pET15b vector for expression in Escherichia coli cells	Homo sapiens	-	671066
cloned into the pQE32 vector for expression in Escherichia coli M15 cells	Kluyveromyces marxianus	-	674688
expressed in Escherichia coli JM109 cells	Lentinus polychrous	D3K1B6	710790
expressed in Escherichia coli XL1-blue cells transformed with pNeis2	Neisseria meningitidis	-	688953
expressed in Escherichia coli strain W3CG and in HeLa cells	Oryctolagus cuniculus	-	687602
expression in sf21 cell	Oryctolagus cuniculus	-	695902
into the TOPO pET100 expression vector for transformation of Escherichia coli XL1-Blue competent cells	Paracoccidioides brasiliensis	Q8X1X3	673986
expression in Escherichia coli	Pinus sylvestris	-	287956
nuclear-encoded, plastid specific enzyme	Pinus sylvestris	-	287922
into the pET14b vector for expression in Escherichia coli BL21 Star cells	Plasmodium falciparum	Q8IKK7	677046
into the pET15b vector for expression in Escherichia coli BL21DE3 cells	Plasmodium falciparum	-	671063
-	Pleurodeles waltl	Q8AXB5	655270
expression in Escherichia coli	Pseudomonas sp.	A7LHM7	698639
-	Rattus norvegicus	-	287925, 698925
expression in INS-1 cells	Rattus norvegicus	P04797	696464
the rat cDNA library is cloned in the target vector pTRG, Escherichia coli is used as a reporter strain	Rattus norvegicus	-	674503
-	Saccharomyces cerevisiae	-	287952, 287954
-	Sinapis alba	-	287923
a 1200 bp fragment of the phosphorylating GAPC encoding cDNA is cloned in the antisense orientation under control of the CaMV 35S promoter into the plasmid pBinAR for transformation of Agrobacterium tumefaciens	Solanum tuberosum	Q8LK04	675137
-	Staphylococcus aureus	Q6GIL8	695363
expressed in Escherichia coli M15 cells	Staphylococcus aureus	-	710757
a 1008 bp DNA fragment from the plr-sdh-gapdh gene is ligated into the vector pGEX-6P-1 for expression of the protein in Escherichia coli BL212DE3 cells	Streptococcus pyogenes	P0C0G6	674559
-	Trypanosoma cruzi	-	684427
two genes for glyceraldehyde-3-phosphate dehydrogenase	Trypanosoma cruzi	-	287912
-	Xenopus laevis	-	655270
-	Zymomonas mobilis	-	287916

EXPRESSION	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
isoform GAPC-1, expression is strong and ubiquitous, insensitive to dark treatments, and unaffected by sucrose; isoform GAPCp-1, expression is ubiquitous, but at lower level than expression of cytosolic isoform GAPC-1. Expression in leaf slowly decreases in the dark and is stable in sucrose-treated leaves	Arabidopsis thaliana	-	656414
GAPDH transcript levels are greatly increased by H2O2 treatment	Arabidopsis thaliana	-	700672
GAPDH-small interfering RNA knockdown sensitizes the cells to methyl methane sulfonate and bleomycin, which generate lesions that are repaired by APE1, but cells show normal sensitivity to 254-nm UV. GAPDH knockdown cells exhibit an increased level of spontaneous abasic sites in the genomic DNA as a result of diminished APE1 endonuclease activity	Homo sapiens	-	698825
24 h after irradiation at 5 gray, nuclear GAPDH levels increase 2.6fold, whereas total GAPDH increases only 1.2fold. Knockdown of GAPDH by siRNA leads to sensitization to X-ray-induced cell death	Homo sapiens	-	696824
heat (45�C) and cold (10�C) treatments for 6 h increase the expression level of GAPDH gene approximately 6fold, whereas ethanol (6% (v/v)) and salt (1 M) treatments for 6 h increase the expression of the GAPDH gene about 3fold, as compared to the control condition	Lentinus polychrous	D3K1B6	710790
transcription of GAPDH1 is affected by neither bacterial like Escherichia coli, Edwardsiella tarda, Vibrio anguillarum or Streptococcus iniae nor viral such as rockbream iridovirus, RBIV challenges	Oplegnathus fasciatus	B5AAJ5, B5AAJ6	698019
mRNA expression of rbGAPDH2 is up to 25fold up-regulated in liver resulting from bacterial infections andmore than sixfold upregulated in liver and up to fivefold in kidney upon viral infection	Oplegnathus fasciatus	B5AAJ5, B5AAJ6	698019

ENGINEERING	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
C153S	Arabidopsis thaliana	-	mutant, having only one cysteine in the catalytic site, is oxidized and glutathionylated similarly to the wild-type enzyme	673587
D32A	Bacillus subtilis	-	activity of mutant enzyme D32A with NAD+ is equal to that of the wild-type enzyme, mutant enzyme also shows activity with NADP+, about 3% of the activity with NAD+	287980
D32A/L187N	Bacillus subtilis	-	wild-type enzyme has no activity with NADP+, the mutant enzyme D32A/L187N shows catalytic efficiency with NADP+ higher than that with NAD+	287980
L187N	Bacillus subtilis	-	activity of mutant L187N with NAD+ is higher than that of the wild-type enzyme, mutant enzyme also shows activity with NADP+, about 7% of the activity with NAD+	287980
C153S	Cryptosporidium parvum	-	about 450fold decrease in activity	697008
C153S	Cryptosporidium parvum	-	the specific activity of the mutant enzyme is approximately 450fold lower than that of the wild type enzyme	711617
N313T/Y317G	Escherichia coli	P0A9B2	dissociation constant for NAD+ is 300times higher than that of the wild-type enzyme. Conformational equilibrium between the syn and the anti forms with a preference for the anti conformer	287927
Y317A	Escherichia coli	P0A9B2	dissociation constant for NAD+ is 5times higher than that of the wild-type enzyme. Wild-type syn orientation of bound nicotinamide remains unchanged	287927
Y317G	Escherichia coli	P0A9B2	dissociation constant for NAD+ is 13times higher than that of the wild-type enzyme. Wild-type syn orientation of bound nicotinamide remains unchanged	287927
C149A	Geobacillus stearothermophilus	-	mutant enzyme displays no significant dehydrogenase activity	656119
C149S	Geobacillus stearothermophilus	-	low but significant phosphorylating dehydrogenase activity	656119
C149selenocysteine	Geobacillus stearothermophilus	-	mutant enzyme has selenoperoxidase activity	287984
D186G	Geobacillus stearothermophilus	-	behavior in NAD+ binding is similar to that of the wild type enzyme	287985
D186G/E276G	Geobacillus stearothermophilus	-	positive cooperativity in binding the coenzyme NAD+	287985
D282G	Geobacillus stearothermophilus	-	enzyme exists as dimer and tetramer, the tetramer is inactive, the dimer is slightly active, 650fold decrease in turnover number for NAD+, 5.8fold increase in Km-value for NAD+ compared to wild-type enzyme	654653
E276G	Geobacillus stearothermophilus	-	behavior in NAD+ binding is similar to that of the wild type enzyme	287985
L187A/P188S	Geobacillus stearothermophilus	-	the mutant is catalytically active not only with NAD+, as the wild-type enzyme, but also with NADP+	287909
N313T	Geobacillus stearothermophilus	-	mutant enzyme with a drastic decrease in thermostability, weakening of cooperative interactions between the catalytic and the cofactor domains and an inefficient binding of NAD+, mutant enzyme exists only as tetramer, 65fold decrease in turnover number for NAD+, 50fold increase in Km-value for NAD+ compared to wild-type enzyme	654653
T34Q/T39S/L43Q	Geobacillus stearothermophilus	-	drastic decrease in thermostability, inefficient NAD+ binding, enzyme exists as dimer and tetramer, the tetramer is inactive, the dimer is slightly active, 650fold decrease in turnover number for NAD+, 4fold increase in Km-value for NAD+ compared to wild-type enzyme	654653
W310F	Geobacillus stearothermophilus	-	mutant enzyme with a drastic decrease in thermostability, mutant enzyme exists only as tetramer, 2fold increase of Km-value for NAD+, 1.3fold decrease in turnover number compared to wild-type enzyme	654653
W84F	Geobacillus stearothermophilus	-	slightly lower Km-values for NAD+ and glyceraldehyde 3-phosphate, slightly higher Km-value for phosphate. The construction of the mutant permitts the identification of the individual fluorescence and phosphorescence characteristics of the two Trp residues W84 and W310 in the native enzyme	287947
Y283V	Geobacillus stearothermophilus	-	mutant enzyme with a drastic decrease in thermostability, dimeric form is inactive, KM-value and turnover-number of tetramer are nearly identical to that of the wild-type	654653
Y46G	Geobacillus stearothermophilus	-	behavior in NAD+ binding is similar to that of the wild type enzyme	287985
Y46G/R52G	Geobacillus stearothermophilus	-	inactive mutant enzyme, only exists as dimer	654653
Y46G/S48G	Geobacillus stearothermophilus	-	positive cooperativity in binding the coenzyme NAD+	287985
Y46G/S48G	Geobacillus stearothermophilus	-	inactive mutant enzyme, only exists as dimer	654653
C149A	Homo sapiens	-	mutant has almost completely lost the ability to bind telomere. Upon expression in A-549 cells, mutant localizes to the nucleus but is unable to confer any significant protection of telomeres against chemotherapy-induced degradation or growth inhibition	699592
C152G	Homo sapiens	-	mutant retains the ability to interact with but is unable to reactivate DNA repair enzyme APE1	698825
C156G	Homo sapiens	-	mutant retains the ability to interact with but is unable to reactivate DNA repair enzyme APE1	698825
A229V	Mus musculus	-	exhibits 50-55% residual activity in blood compared to the wild type enzyme	688791
C281W	Mus musculus	-	exhibits 50-55% residual activity in blood compared to the wild type enzyme	688791
G166D	Mus musculus	-	exhibits 50-55% residual activity in blood compared to the wild type enzyme	688791
V239I	Mus musculus	-	exhibits 50-55% residual activity in blood compared to the wild type enzyme	688791
Y327N	Mus musculus	-	exhibits 50-55% residual activity in blood compared to the wild type enzyme	688791
C149S	Oryctolagus cuniculus	-	treatment of with (E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide at 0.1 mM leads to low levels of aggregation (5% of wild type)	687602
C149S/C281S	Oryctolagus cuniculus	-	mutant shows a complete absence of aggregation in the presence of (E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide	687602
C153S	Oryctolagus cuniculus	-	aggregation can be detected at low concentrations of (E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide (0.001 mM) and is enhanced at higher concentrations	687602
C244A	Oryctolagus cuniculus	-	aggregation can be detected at low concentrations of (E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide (0.001 mM) and is enhanced at higher concentrations	687602
C281S	Oryctolagus cuniculus	-	the levels of aggregation in C281S are reduced to 45% of wild type at 0.1 mM (E)-4-ethyl-2-[(E)-hydroxyimino]-5-nitro-3-hexenamide	687602
T227A	Rattus norvegicus	P04797	mutation at site of O-GlcNAcylation. Mutation induces the cytoplasmic accumulation of glyceraldehyde 3-phosphate dehydrogenase	696464
C151/H178N	Staphylococcus aureus	-	the rate of the forward reaction is decreased by 47000 times compared to the wild type enzyme with similar affinity for the substrate and the coenzyme	712766
C151G	Staphylococcus aureus	-	the mutant is completely inactive	712766
C151S	Staphylococcus aureus	-	the mutant shows drastically reduced kcat values compared to the wild type enzyme	712766
H178N	Staphylococcus aureus	-	the mutant shows no significant difference in the Km values of D-glyceraldehyde 3-phosphate, phosphate, and NAD+ compared to the wild type enzyme, however, the mutation results in the reduction of kcat of oxidative phosphorylation by 1400times	712766
C159S	Arabidopsis thaliana	P25858, Q56WJ4	the mutant C159S of the isozyme GapC2 shows decreased specific activity	689409
additional information	Arabidopsis thaliana	-	GAPC-1 antisense line shows a delay in growth, morpholigical alterations in siliques, and low seed number. Embryo development is altered, showing abortions and empty embryonic sacs in basal and apical siliques, respectively. Mutant shows a decrease in the expression and activity of aconitase and succinate dehydrogenase and reduced levels of pyruvate and several Krebs cycle intermediates, and increased reactive oxygen species levels	700811
C149A	Escherichia coli	-	mutation abolishes NAD+-dependent ADP-ribosylation of the enzyme	698374
additional information	Escherichia coli	-	replacement of Escherichia coli GapA glyceraldehyde 3-phosphate dehydrogenase by Clostridium acetobutylicum GapC glyceraldehyde 3-phosphate dehydrogenase, EC 1.2.1.9 results in significant reduction of flux through the pentose phosphate pathway. Recombinant strains display increased NADPH availability, and consistently higher productivity than parent strains	699988
D32A	Homo sapiens	-	mutant is unable to bind NAD+, is enzymatically inactive and has almost completely lost the ability to bind telomere. Upon expression in A-549 cells, mutant localizes to the nucleus but is unable to confer any significant protection of telomeres against chemotherapy-induced degradation or growth inhibition	699592
additional information	Homo sapiens	-	mutant htt shows co-localization of GAPDH with N-terminus of huntingtin aggregates	684120
I308S	Mus musculus	-	exhibits 50-55% residual activity in blood compared to the wild type enzyme	688791
additional information	Mus musculus	-	mutants Gapdhm1Neu (Y91stop truncated protein of 89 amino acids), Gapdhm2Neu (truncated/altered protein of 9 amino acids), and Gapdhm8Neu (truncated/altered protein of 73 amino acids) exhibit 50-55% residual activity in blood compared to the wild type enzyme	688791

Renatured/COMMENTARY	ORGANISM	UNIPROT ACCESSION NO.	LITERATURE
GroEL-assisted reactivation after denaturation of GAPDH in the presence of guanidine hydrochloride	Oryctolagus cuniculus	-	672402
lysis buffer contains 6 M guanidine hydrochloride, during purification bound proteins are eluted with native elution buffer to refold the protein	Paracoccidioides brasiliensis	Q8X1X3	673986

APPLICATION	ORGANISM	UNIPROT ACCESSION NO.	COMMENTARY	LITERATURE
medicine	Homo sapiens	-	functions are of chemotherapeutic interest, structure is a necessity for structure-based drug design	671066
medicine	Homo sapiens	-	target for the design of antitrypanosomatid and anti-apoptosis drugs	671070
medicine	Homo sapiens	-	pathogenesis of Porphyromonas gingivalis involves interaction with oral mucosal epithelial cells, mediated by binding glyceraldehyde-3-phosphate dehydrogenase	673706
medicine	Homo sapiens	-	action as a selective insulin receptor modulator	688250
medicine	Homo sapiens	-	glyceraldehyde 3-phosphate dehydrogenase may be involved in the postranscriptional regulation of hepatitis B virus. Recombinant glyceraldehyde 3-phosphate dehydrogenase binds to hepatitis B virus regulatory element RNA in vitro and inhibits hepatitis B virus regulatory element function. Overexpression of glyceraldehyde 3-phosphate dehydrogenase depresses the expression of hepatitis B virus antigen	695939
medicine	Homo sapiens	-	glyceraldehyde 3-phosphate dehydrogenase from malignant sources shows altered properties compared with enzyme from healthy subjects	696174
medicine	Homo sapiens	-	among 256 ovarian and fallopian tube cancer specimens, GAPDH is regulated, with almost 50% of specimens having no GAPDH staining.Low GAPDH staining is associated with a low macrophage colony stimulating factor CSF-1 score	700133
medicine	Mus musculus	-	in experimental autoimmune encephalomyelitis, the proportion of S-nitrosylated neurofilament proteins, NMDA receptors, alpha/beta-tubulin, beta-actin, and GAPDH is increased. Neuronal specific enolase is the major S-nitrosylated protein in acute experimental autoimmune encephalomyelitis	699686
medicine	Oryctolagus cuniculus	-	pathogenesis of Porphyromonas gingivalis involves interaction with oral mucosal epithelial cells, mediated by binding glyceraldehyde-3-phosphate dehydrogenase	673706
medicine	Oryctolagus cuniculus	-	non-native forms of GAPDH may be involved in the formation of amyloid structures during Alzheimer's disease, binding to soluble amyloid-beta peptide	696421
medicine	Paracoccidioides brasiliensis	Q8X1X3	the pathogenic fungus causes paracoccidioidomycosis, GAPDH is reported as an adhesin, which can be related to fungus adhesion and invasion	673986
medicine	Plasmodium brasilianum	B0F3R1	G3PDH is a potential target for antimalarial drug development	687069
medicine	Plasmodium coatneyi	B0F3R2	G3PDH is a potential target for antimalarial drug development	687069
medicine	Plasmodium cynomolgi	B0F3R8	G3PDH is a potential target for antimalarial drug development	687069
medicine	Plasmodium falciparum	-	potential antimalarial target	671063
medicine	Plasmodium falciparum	Q8IKK7	crystallography may provide structural detail for the development of lead compounds and drug design of novel antimalarials	677046
medicine	Plasmodium falciparum	B0F3R3	G3PDH is a potential target for antimalarial drug development	687069
medicine	Plasmodium fragile	B0F3R4	G3PDH is a potential target for antimalarial drug development	687069
medicine	Plasmodium knowlesi strain H	B0F3R5	G3PDH is a potential target for antimalarial drug development	687069
medicine	Plasmodium malariae	B0F3R6	G3PDH is a potential target for antimalarial drug development	687069
medicine	Plasmodium reichenowi	B0F3R7	G3PDH is a potential target for antimalarial drug development	687069
medicine	Plasmodium vivax	B0F3R9	G3PDH is a potential target for antimalarial drug development	687069
medicine	Rattus norvegicus	-	in diabetic rats, modification of GAPDH by S-(2-succinyl)cysteine is increased in muscle, and the extent of succination correlates strongly with the decrease in specific activity of the enzyme	695595
pharmacology	Schistosoma mansoni	-	lung-stage schistosomula immunofluorescence reactivity is diminished following antiserum absorption with reconbinant glyceraldehyde 3-phosphate dehydrogenase. Discussion of glyceraldehyde 3-phosphate dehydrogenase as a candidate vaccine antigen	698033
medicine	Streptococcus pyogenes	P0C0G6	the Plr-SDH-GAPDH complex participates in invasive streptococcal infections	674559

DISEASE	TITLE OF PUBLICATION	LINK TO PUBMED
6-phosphofructokinase deficiency	Iodoacetate inhibition of glyceraldehyde-3-phosphate dehydrogenase as a model of human myophosphorylase deficiency (McArdle's disease) and phosphofructokinase deficiency (Tarui's disease).	PubMed
Acidosis	Effects of ketoacidosis on rat apolipoprotein A1 gene expression: a link with acidosis but not with ketones.	PubMed
Acidosis	Effects of treatment with pyruvate and tromethamine in experimental myocardial ischemia.	PubMed
Acidosis, Lactic	Regulation of glyceraldehyde 3-phosphate dehydrogenase expression by metformin in HepG2 cells.	PubMed
Acquired Immunodeficiency Syndrome	Haemophilus influenzae uses the surface protein E to acquire human plasminogen and to evade innate immunity.	PubMed
ACTH-Secreting Pituitary Adenoma	Expression of the pituitary transcription factor Ptx-1, but not that of the trans-activating factor prop-1, is reduced in human corticotroph adenomas and is associated with decreased alpha-subunit secretion.	PubMed
Acute Kidney Injury	The modifier protein of glyceraldehyde-3-phosphate dehydrogenase reduces free radical-mediated renal damage in a rat model of acute kidney injury.	PubMed
Adenocarcinoma	Association of glyceraldehyde-3-phosphate dehydrogenase expression with cell motility and metastatic potential of rat prostatic adenocarcinoma.	PubMed
Adenocarcinoma	Inactivation of metabolic enzymes by photo-treatment with zinc meta N-methylpyridylporphyrin.	PubMed
Adenocarcinoma	Increased expression of glycolysis-associated genes in oncogene-transformed and growth-accelerated states.	PubMed
Adenocarcinoma	Increased glyceraldehyde-3-phosphate dehydrogenase gene expression in human pancreatic adenocarcinoma.	PubMed
Adenocarcinoma	The cytotoxic ribonuclease onconase targets RNA interference (siRNA).	PubMed
Adenoma	Bim-23244, a somatostatin receptor subtype 2- and 5-selective analog with enhanced efficacy in suppressing growth hormone (GH) from octreotide-resistant human GH-secreting adenomas.	PubMed
Adenoma	Histochemical profile of mouse hepatocellular adenomas and carcinomas induced by a single dose of diethylnitrosamine.	PubMed
Alkalosis	Effects of ketoacidosis on rat apolipoprotein A1 gene expression: a link with acidosis but not with ketones.	PubMed
alpha-Thalassemia	A New 5' Terminal Murine GAPDH Exon Identified Using 5'RACE LaNe.	PubMed
Alzheimer Disease	Association and heterogeneity at the GAPDH locus in Alzheimer's disease.	PubMed
Alzheimer Disease	Decrease of dehydrogenase activity of cerebral glyceraldehyde-3-phosphate dehydrogenase in different animal models of Alzheimer's disease.	PubMed
Alzheimer Disease	Exploring the association of glyceraldehyde-3-phosphate dehydrogenase gene and Alzheimer disease.	PubMed
Alzheimer Disease	Isolation of glyceraldehyde 3-phosphate dehydrogenase (Gapdh) cDNA from the distal half of mouse chromosome 16: further indication of a link between Alzheimer's disease and glycolysis.	PubMed
Alzheimer Disease	Oxidatively Modified Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH) and Alzheimer's Disease: Many Pathways to Neurodegeneration.	PubMed
Alzheimer Disease	Quantifying mRNA in postmortem human brain: influence of gender, age at death, postmortem interval, brain pH, agonal state and inter-lobe mRNA variance.	PubMed
Alzheimer Disease	Reduction of glyceraldehyde-3-phosphate dehydrogenase activity in Alzheimer's disease and in Huntington's disease fibroblasts.	PubMed
Alzheimer Disease	Subcellular alteration of glyceraldehyde-3-phosphate dehydrogenase in Alzheimer's disease fibroblasts.	PubMed
Amyotrophic Lateral Sclerosis	Determination of protein and RNA expression levels of common housekeeping genes in a mouse model of neurodegeneration.	PubMed
Anaphylaxis	Glyceraldehyde-3-phosphate dehydrogenase as a major allergen in rambutan-induced anaphylaxis.	PubMed
Anemia	Increased membrane activity of glyceraldehyde 3-phosphate dehydrogenase in erythrocytes of patients with homozygous sickle cell anaemia.	PubMed
Anemia	[Activity of the enzymes phosphoglyceraldehyde dehydrogenase, lactate dehydrogenase and glucose-6-phosphate dehydrogenase in the erythrocytes in deficiency anemia]	PubMed
Anemia, Sickle Cell	Increased membrane activity of glyceraldehyde 3-phosphate dehydrogenase in erythrocytes of patients with homozygous sickle cell anaemia.	PubMed
Anthrax	Surface localized and extracellular Glyceraldehyde-3-phosphate dehydrogenase of Bacillus anthracis is a plasminogen binding protein.	PubMed
Arthritis	Oxidation of articular cartilage glyceraldehyde-3-phosphate dehydrogenase (G3PDH) occurs in vivo during carrageenin-induced arthritis.	PubMed
Avitaminosis	Vitamin B6 deficiency causes activation of RNA polymerase and general enhancement of gene expression in rat liver.	PubMed
Azoospermia	Quantification of cyclin A1 and glyceraldehyde-3-phosphate dehydrogenase expression in testicular biopsies of infertile patients by fluorescence real-time RT-PCR.	PubMed
Bacterial Infections	Determination of internal controls for quantitative real time RT-PCR analysis of the effect of Edwardsiella tarda infection on gene expression in turbot (Scophthalmus maximus).	PubMed
Bacterial Infections	Inhibition of IL-10 production by maternal antibodies against Group B Streptococcus GAPDH confers immunity to offspring by favoring neutrophil recruitment.	PubMed
beta-Thalassemia	Analysis of Erythrocyte and Platelet Membrane Proteins in Various Forms of beta-Thalassemia.	PubMed
Breast Neoplasms	Cell-surface cytokeratin 8 is the major plasminogen receptor on breast cancer cells and is required for the accelerated activation of cell-associated plasminogen by tissue-type plasminogen activator.	PubMed
Breast Neoplasms	Characterization of transport protein expression in multidrug resistance-associated protein (Mrp) 2-deficient rats.	PubMed
Breast Neoplasms	Comparative expression of fibroblast growth factor mRNAs in benign and malignant breast disease.	PubMed
Breast Neoplasms	Glyceraldehyde-3-phosphate dehydrogenase as a surface associated antigen on human breast cancer cell lines MACL-1 and MGSO-3.	PubMed
Breast Neoplasms	Glyceraldehyde-3-phosphate dehydrogenase gene expression in human breast cancer.	PubMed
Breast Neoplasms	Glycolaldehyde induces apoptosis in a human breast cancer cell line.	PubMed
Breast Neoplasms	Measurement of mRNA levels in tumor xenografts with quantitative autoradiography and in situ hybridization.	PubMed
Breast Neoplasms	Parallel assessment of circulatory cell-free DNA by PCR and nucleosomes by ELISA in breast tumors.	PubMed
Breast Neoplasms	Up-regulation of glyceraldehyde-3-phosphate dehydrogenase gene expression by HIF-1 activity depending on Sp1 in hypoxic breast cancer cells.	PubMed
Breast Neoplasms	Visualization of the plasmin receptor on sections of human mammary carcinoma cells.	PubMed
Brucellosis	Brucella abortus L7/L12 recombinant protein induces strong Th1 response in acute brucellosis patients.	PubMed
Burkitt Lymphoma	The mode of actions of glyceraldehyde-3-phosphate dehydrogenase identified as an immunoglobulin production stimulating factor.	PubMed
Candidiasis	Synthetic glycopeptide vaccines combining beta-mannan and peptide epitopes induce protection against candidiasis.	PubMed
Candidiasis, Invasive	Evaluation of the usefulness of anti-glyceraldehyde-3-phosphate dehydrogenase antibodies as a treatment for invasive candidiasis in a murine model.	PubMed
Carcinoma	Alteration of glyceraldehyde-3-phosphate dehydrogenase activity and messenger RNA content by androgen in human prostate carcinoma cells.	PubMed
Carcinoma	Antisense oligodeoxynucleotide of glyceraldehyde-3-phosphate dehydrogenase gene inhibits cell proliferation and induces apoptosis in human cervical carcinoma cell lines.	PubMed
Carcinoma	Chromatin-associated proteins HMGB1/2 and PDIA3 trigger cellular response to chemotherapy-induced DNA damage.	PubMed
Carcinoma	Comparative proteomic analysis of esophageal squamous cell carcinoma.	PubMed
Carcinoma	Cytokeratin 8 released by breast carcinoma cells in vitro binds plasminogen and tissue-type plasminogen activator and promotes plasminogen activation.	PubMed
Carcinoma	Enhanced gene expression in breast cancer cells in vitro and tumors in vivo.	PubMed
Carcinoma	Enzymes of carbohydrate metabolism in human breast carcinoma: relationship with serum hormones.	PubMed
Carcinoma	Evidence for the presence of a critical histidine residue at the active site in glyceraldehyde-3-phosphate dehydrogenase of Ehrlich ascites carcinoma cells.	PubMed
Carcinoma	Expression of human prostatic acid phosphatase and prostate specific antigen genes in neoplastic and benign tissues.	PubMed
Carcinoma	Gene expression profiles in thyroid carcinomas.	PubMed
Carcinoma	Glyceraldehyde 3-phosphate dehydrogenase depletion induces cell cycle arrest and resistance to antimetabolites in human carcinoma cell lines.	PubMed
Carcinoma	Glyceraldehyde-3-phosphate dehydrogenase from Ehrlich ascites carcinoma cells its possible role in the high glycolysis of malignant cells.	PubMed
Carcinoma	Hepatocellular glycogenosis and related pattern of enzymatic changes during hepatocarcinogenesis.	PubMed
Carcinoma	Histochemical profile of mouse hepatocellular adenomas and carcinomas induced by a single dose of diethylnitrosamine.	PubMed
Carcinoma	Identification of a critical lysine residue at the active site in glyceraldehyde-3-phosphate dehydrogenase of Ehrlich ascites carcinoma cell. Comparison with the rabbit muscle enzyme.	PubMed
Carcinoma	Increased glyceraldehyde-3-phosphate dehydrogenase expression in renal cell carcinoma identified by RNA-based, arbitrarily primed polymerase chain reaction.	PubMed
Carcinoma	Interaction of cytotoxic antibiotic dactylarin with glycolytic thiol enzymes in Ehrlich ascites carcinoma cells.	PubMed
Carcinoma	Purification of the plasmin receptor from human carcinoma cells and comparison to alpha-enolase.	PubMed
Carcinoma	Reversible oxidation of glyceraldehyde 3-phosphate dehydrogenase thiols in human lung carcinoma cells by hydrogen peroxide.	PubMed
Carcinoma	Solubilization of the plasmin receptor from human carcinoma cells.	PubMed
Carcinoma	Specific inhibition of glyceraldehyde-3-phosphate dehydrogenase by koningic acid (heptelidic acid).	PubMed
Carcinoma	The bioenergetic signature of cancer: a marker of tumor progression.	PubMed
Carcinoma	The prognostic value of tetranectin immunoreactivity and plasma tetranectin in patients with ovarian cancer.	PubMed
Carcinoma	Visualization of the plasmin receptor on carcinoma cells.	PubMed
Carcinoma	Visualization of the plasmin receptor on sections of human mammary carcinoma cells.	PubMed
Carcinoma	[Proteomic identification and comparison of differentiation-related proteins in gastric carcinoma cell lines]	PubMed
Carcinoma, Ehrlich Tumor	Interaction of calcyclin and its cyanogen bromide fragments with annexin II and glyceraldehyde 3-phosphate dehydrogenase.	PubMed
Carcinoma, Ehrlich Tumor	Properties of the glyceraldehyde-3-phosphate dehydrogenase from Ehrlich ascites tumor cells.	PubMed
Carcinoma, Hepatocellular	Comparison of glyceraldehyde-3-phosphate dehydrogenase and 28s-ribosomal RNA gene expression in human hepatocellular carcinoma.	PubMed
Carcinoma, Hepatocellular	Glyceraldehyde-3-phosphate dehydrogenase mRNA expression in hepatocellular carcinoma.	PubMed
Carcinoma, Hepatocellular	Glyceraldehyde-3-Phosphate Dehydrogenase: A Promising Target for Molecular Therapy in Hepatocellular Carcinoma.	PubMed
Carcinoma, Hepatocellular	Human hepatocellular carcinoma in a mouse model: assessment of tumor response to percutaneous ablation by using glyceraldehyde-3-phosphate dehydrogenase antagonists.	PubMed
Carcinoma, Hepatocellular	Insulin stimulates glyceraldehyde-3-phosphate dehydrogenase gene expression through cis-acting DNA sequences.	PubMed
Carcinoma, Hepatocellular	Targets of 3-Bromopyruvate, a New, Energy Depleting, Anticancer Agent.	PubMed
Carcinoma, Non-Small-Cell Lung	Choice of endogenous control for gene expression in nonsmall cell lung cancer.	PubMed
Carcinoma, Renal Cell	Increased glyceraldehyde-3-phosphate dehydrogenase expression in renal cell carcinoma identified by RNA-based, arbitrarily primed polymerase chain reaction.	PubMed
Carcinoma, Squamous Cell	Comparative proteomic analysis of esophageal squamous cell carcinoma.	PubMed
Cardiomyopathy, Dilated	Identification and expression of delta-isoforms of the multifunctional Ca2+/calmodulin-dependent protein kinase in failing and nonfailing human myocardium.	PubMed
Cataract	Revival of inactive glyceraldehyde 3-phosphate dehydrogenase in human cataract lenses by reduction.	PubMed
Cataract	Thioredoxin, thioredoxin reductase, and alpha-crystallin revive inactivated glyceraldehyde 3-phosphate dehydrogenase in human aged and cataract lens extracts.	PubMed
Chagas Disease	3D QSAR studies on binding affinities of coumarin natural products for glycosomal GAPDH of Trypanosoma cruzi.	PubMed
Chagas Disease	Development and characterization of an immobilized enzyme reactor (IMER) based on human glyceraldehyde-3-phosphate dehydrogenase for on-line enzymatic studies.	PubMed
Chagas Disease	Discovery of novel Trypanosoma cruzi glyceraldehyde-3-phosphate dehydrogenase inhibitors.	PubMed
Chagas Disease	Expression, purification and kinetic characterization of His-tagged glyceraldehyde-3-phosphate dehydrogenase from Trypanosoma cruzi.	PubMed
Chagas Disease	Trypanosoma cruzi glycosomal glyceraldehyde-3-phosphate dehydrogenase: structure, catalytic mechanism and targeted inhibitor design.	PubMed
Choriocarcinoma	Nuclear beta-catenin and Ki-67 expression in choriocarcinoma and its pre-malignant form.	PubMed
Colorectal Neoplasms	[Expression of osteopontin mRNA and its protein in colorectal cancer and liver metastatic tissues]	PubMed
Congenital Hypothyroidism	Glyceraldehyde-3-phosphate dehydrogenase activity in developing brain during experimental cretinism.	PubMed
Connective Tissue Diseases	Glyceraldehyde 3-phosphate dehydrogenase is a novel autoantigen leading autoimmune responses to proliferating cell nuclear antigen multiprotein complexes in lupus patients.	PubMed
Contracture	Metabolic myopathy produced by acute inhibition of glyceraldehyde-3-phosphate dehydrogenase with ortho-iodosobenzoic acid.	PubMed
Contracture	Metabolic myopathy produced by dinitrofluorobenzene inhibition of creatine phosphokinase.	PubMed
Cystic Fibrosis	Molecular and functional heterogeneity of cholangiocytes from rat liver after bile duct ligation.	PubMed
Dehydration	Antioxidative defense system, pigment composition, and photosynthetic efficiency in two wheat cultivars subjected to drought	PubMed
Dengue	Recombinant dengue virus-like particles from Pichia pastoris: efficient production and immunological properties.	PubMed
Dental Caries	Validation of reference genes for quantitative measurement of immune gene expression in shrimp.	PubMed
Dermatitis, Atopic	Relative importance of IL-4 and IL-13 in lesional skin of atopic dermatitis.	PubMed
Diabetes Complications	Methylglyoxal can modify GAPDH activity and structure.	PubMed
Diabetes Complications	Role of glyceraldehyde 3-phosphate dehydrogenase in the development and progression of diabetic retinopathy.	PubMed
Diabetes Complications	The pathogenesis of diabetic complications: the role of DNA injury and poly(ADP-ribose) polymerase activation in peroxynitrite-mediated cytotoxicity.	PubMed
Diabetes Mellitus, Type 2	Metabolism of [1,3-(13)C]glycerol-1,2,3-tris(methylsuccinate) and glycerol-1,2,3-tris(methyl[2,3-(13)C]succinate) in hepatocytes from Goto-Kakizaki rats.	PubMed
Diabetic Angiopathies	Role of nitrosative stress in the pathogenesis of diabetic vascular dysfunction.	PubMed
Diabetic Retinopathy	Glyceraldehyde 3 phosphate dehydrogenase in retinal microvasculature: Implications for the development and progression of diabetic retinopathy.	PubMed
Diabetic Retinopathy	Role of glyceraldehyde 3-phosphate dehydrogenase in the development and progression of diabetic retinopathy.	PubMed
Down Syndrome	Increased glyceraldehyde 3-phosphate dehydrogenase levels in the brain of patients with Down's syndrome.	PubMed
Down Syndrome	[Rapid diagnosis of Down's syndrome by multiplex real-time fluorescence relative quantitative PCR]	PubMed
Encephalitis, Japanese	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) interaction with 3' ends of Japanese encephalitis virus RNA and colocalization with the viral NS5 protein.	PubMed
Fatty Liver	Peroxisome proliferator-activated receptor agonist treatment of alcohol-induced hepatic insulin resistance.	PubMed
Fetal Diseases	Maternal diabetes in vivo and high glucose in vitro diminish GAPDH activity in rat embryos.	PubMed
Fibroma	Regulation of proliferation and platelet-derived growth factor expression in palmar fibromatosis (Dupuytren contracture) by mechanical strain.	PubMed
Ganglioneuroblastoma	Expression of the smg p25A (a ras p21-like GTP-binding protein) gene in human neuroblastoma cell lines and tumor tissues.	PubMed
Ganglioneuroblastoma	Real-time analysis of tyrosine hydroxylase gene expression: a sensitive and semiquantitative marker for minimal residual disease detection of neuroblastoma.	PubMed
Ganglioneuroma	Real-time analysis of tyrosine hydroxylase gene expression: a sensitive and semiquantitative marker for minimal residual disease detection of neuroblastoma.	PubMed
Glioma	Histone deacetylase inhibitor 4-phenylbutyrate suppresses GAPDH mRNA expression in glioma cells.	PubMed
Glioma	Quantitative reverse transcription-PCR measurement of tissue factor mRNA in glioma.	PubMed
Glomerulonephritis	A prolonged course of Group A streptococcus-associated nephritis: a mild case of dense deposit disease (DDD)?	PubMed
Glomerulonephritis	Elevated urinary plasmin activity resistant to alpha2-antiplasmin in acute poststreptococcal glomerulonephritis.	PubMed
Glomerulonephritis	Glomerular plasmin-like activity in relation to nephritis-associated plasmin receptor in acute poststreptococcal glomerulonephritis.	PubMed
Glomerulonephritis	Group A streptococcal antigen in the glomeruli of children with Henoch-Schönlein nephritis.	PubMed
Glomerulonephritis	Increased mRNA expression of metalloproteinase-9 in peripheral blood monocytes from patients with immunoglobulin A nephropathy.	PubMed
Glomerulonephritis	Is the nephritogenic antigen in post-streptococcal glomerulonephritis pyrogenic exotoxin B (SPE B) or GAPDH?	PubMed
Glomerulonephritis	Localization of nephritis-associated plasmin receptor in acute poststreptococcal glomerulonephritis.	PubMed
Glomerulonephritis	Nephritis-associated plasmin receptor and acute poststreptococcal glomerulonephritis: characterization of the antigen and associated immune response.	PubMed
Glomerulonephritis	Sequence and expression of NAPlr is conserved among group A streptococci isolated from patients with acute poststreptococcal glomerulonephritis (APSGN) and non-APSGN.	PubMed
Glomerulonephritis	The potential role for nephritis-associated plasmin receptor in acute poststreptococcal glomerulonephritis.	PubMed
Glomerulonephritis, IGA	Increased mRNA expression of metalloproteinase-9 in peripheral blood monocytes from patients with immunoglobulin A nephropathy.	PubMed
Glomerulonephritis, Membranoproliferative	A case of idiopathic membranoproliferative glomerulonephritis with a transient glomerular deposition of nephritis-associated plasmin receptor antigen.	PubMed
Glomerulonephritis, Membranous	Increased mRNA expression of metalloproteinase-9 in peripheral blood monocytes from patients with immunoglobulin A nephropathy.	PubMed
Glomerulonephritis, Membranous	Th2 cytokines increase and stimulate B cells to produce IgG4 in idiopathic membranous nephropathy.	PubMed
Glomerulosclerosis, Focal Segmental	Increased mRNA expression of metalloproteinase-9 in peripheral blood monocytes from patients with immunoglobulin A nephropathy.	PubMed
glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) deficiency	A simple screening procedure for glucose phosphate isomerase, phosphofructokinase, aldolase and glyceraldehyde-3-phosphate dehydrogenase deficiencies.	PubMed
glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) deficiency	Plastidial glyceraldehyde-3-phosphate dehydrogenase deficiency leads to altered root development and affects the sugar and amino Acid balance in Arabidopsis.	PubMed
glyceraldehyde-3-phosphate dehydrogenase (phosphorylating) deficiency	Study of a kindred with hereditary spherocytosis and glyceraldehyde-3-phosphate dehydrogenase deficiency.	PubMed
Glycogen Storage Disease Type V	Iodoacetate inhibition of glyceraldehyde-3-phosphate dehydrogenase as a model of human myophosphorylase deficiency (McArdle's disease) and phosphofructokinase deficiency (Tarui's disease).	PubMed
Heart Arrest	Selection of reference genes for quantitative real-time PCR in a rat asphyxial cardiac arrest model.	PubMed
Heart Failure	Expression of proinflammatory cytokines in the failing human heart: comparison of recent-onset and end-stage congestive heart failure.	PubMed
Heart Failure	Proteomic analysis of left ventricular remodeling in an experimental model of heart failure.	PubMed
Heart Failure	Reduced activity of enzymes coupling ATP-generating with ATP-consuming processes in the failing myocardium.	PubMed
Hemochromatosis	Localization of iron metabolism-related mRNAs in rat liver indicate that HFE is expressed predominantly in hepatocytes.	PubMed
Hepatitis	Specific interaction between the hepatitis delta virus RNA and glyceraldehyde 3-phosphate dehydrogenase: an enhancement on ribozyme catalysis.	PubMed
Hepatitis A	Functional significance of the interaction of hepatitis A virus RNA with glyceraldehyde 3-phosphate dehydrogenase (GAPDH): opposing effects of GAPDH and polypyrimidine tract binding protein on internal ribosome entry site function.	PubMed
Hepatitis A	Interaction of glyceraldehyde-3-phosphate dehydrogenase with secondary and tertiary RNA structural elements of the hepatitis A virus 3' translated and non-translated regions.	PubMed
Hepatitis A	Specific interaction of glyceraldehyde 3-phosphate dehydrogenase with the 5'-nontranslated RNA of hepatitis A virus.	PubMed
Hepatitis B	A yeast system for stable expression of hepatitis B surface antigen.	PubMed
Hepatitis B	Controlled fed-batch fermentation of recombinant Saccharomyces cerevisiae to produce hepatitis B surface antigen.	PubMed
Hepatitis B	Evaluation of Aspergillus niger as host for virus-like particle production, using the hepatitis B surface antigen as a model.	PubMed
Hepatitis B	Expression of hepatitis B surface antigen in Saccharomyces cerevisiae utilizing glyceraldeyhyde-3-phosphate dehydrogenase promoter of Pichia pastoris.	PubMed
Hepatitis B	Identification of glyceraldehyde-3-phosphate dehydrogenase as a cellular protein that binds to the hepatitis B virus posttranscriptional regulatory element.	PubMed
Hepatitis B	Phosphorylation of the hepatitis B virus core protein by glyceraldehyde-3-phosphate dehydrogenase protein kinase activity.	PubMed
Hepatitis B	Production of hepatitis B virion-like particles in yeast.	PubMed
Hepatitis B	Role of glyceraldehyde-3-phosphate dehydrogenase binding to hepatitis B virus posttranscriptional regulatory element in regulating expression of HBV surface antigen.	PubMed
Hepatitis C	Human hepatic glyceraldehyde-3-phosphate dehydrogenase binds to the poly(U) tract of the 3' non-coding region of hepatitis C virus genomic RNA.	PubMed
Huntington Disease	Alteration of nuclear glyceraldehyde-3-phosphate dehydrogenase structure in Huntington's disease fibroblasts.	PubMed
Huntington Disease	Glyceraldehyde 3-phosphate dehydrogenase abnormality in metabolically stressed Huntington disease fibroblasts.	PubMed
Huntington Disease	Neuronal death in cultured murine cortical cells is induced by inhibition of GAPDH and triosephosphate isomerase.	PubMed
Huntington Disease	Overexpression and nuclear accumulation of glyceraldehyde-3-phosphate dehydrogenase in a transgenic mouse model of Huntington's disease.	PubMed
Huntington Disease	Reduction of glyceraldehyde-3-phosphate dehydrogenase activity in Alzheimer's disease and in Huntington's disease fibroblasts.	PubMed
Hypercholesterolemia	Effects and mechanism of turmeric vasorelaxation of the thoracic aorta in hypercholesterolemic rats.	PubMed
Hyperglycemia	Hyperglycemia-induced mitochondrial superoxide overproduction activates the hexosamine pathway and induces plasminogen activator inhibitor-1 expression by increasing Sp1 glycosylation.	PubMed
Hyperglycemia	Sirtuin 1-mediated cellular metabolic memory of high glucose via the LKB1/AMPK/ROS pathway and therapeutic effects of metformin.	PubMed
Hypertension	Influence of pressure overload and ACE inhibitor therapy on constitutive protein mRNA expression in the spontaneously hypertensive rat.	PubMed
Hypotension	[Does nitric oxide stress exist?]	PubMed
Infarction, Middle Cerebral Artery	Glyceraldehyde-3-phosphate dehydrogenase versus toluidine blue as a marker for infarct volume estimation following permanent middle cerebral artery occlusion in mice.	PubMed
Infection	Changes in the contents of metabolites and enzyme activities in rice plants responding to Rhizoctonia solani Kuhn infection: activation of glycolysis and connection to phenylpropanoid pathway.	PubMed
Infection	Differential effects of rabies and borna disease viruses on immediate-early- and late-response gene expression in brain tissues.	PubMed
Infection	Effect of acute infectious disease on human isometric muscle endurance.	PubMed
Infection	Effects of a diabetogenic strain of encephalomyocarditis (EMC) virus on protein synthesis in mouse islets of Langerhans.	PubMed
Infection	Evaluation of reference genes for real-time PCR studies of Brazilian Somalis sheep infected by gastrointestinal nematodes.	PubMed
Infection	Evaluation of the suitability of six host genes as internal control in real-time RT-PCR assays in chicken embryo cell cultures infected with infectious bursal disease virus.	PubMed
Infection	Evaluation of the usefulness of anti-glyceraldehyde-3-phosphate dehydrogenase antibodies as a treatment for invasive candidiasis in a murine model.	PubMed
Infection	Glyceraldehyde-3-phosphate dehydrogenase of Edwardsiella tarda has protective antigenicity against Vibrio anguillarum in Japanese flounder.	PubMed
Infection	Immunisation with Salmonella typhimurium-delivered glyceraldehyde-3-phosphate dehydrogenase protects mice against challenge infection with Echinococcus multilocularis eggs.	PubMed
Infection	Mucosal immunisation with novel Streptococcus pneumoniae protein antigens enhances bacterial clearance in an acute mouse lung infection model.	PubMed
Infection	Quantitative detection for low levels of Helicobacter pylori infection in experimentally infected mice by real-time PCR.	PubMed
Infection	Role of secreted glyceraldehyde-3-phosphate dehydrogenase in the infection mechanism of enterohemorrhagic and enteropathogenic Escherichia coli: interaction of the extracellular enzyme with human plasminogen and fibrinogen.	PubMed
Infection	Secreted glyceraldehyde-3-phosphate dehydrogenase as a broad spectrum vaccine candidate against microbial infection in aquaculture.	PubMed
Infection	Small interfering RNAs that deplete the cellular translation factor eIF4H impede mRNA degradation by the virion host shutoff protein of herpes simplex virus.	PubMed
Infection	The major parasite surface antigen associated with human resistance to schistosomiasis is a 37-kD glyceraldehyde-3P-dehydrogenase.	PubMed
Infection	Translational control of viral and host protein synthesis during the course of herpes simplex virus type 1 infection: evidence that initiation of translation is the limiting step.	PubMed
Infection	Using host 28S ribosomal RNA as a housekeeping gene for quantitative real-time reverse transcription-PCR (qRT-PCR) in virus-infected animal cells.	PubMed
Infection	Vaccine efficacy of recombinant GAPDH of Edwardsiella tarda against Edwardsiellosis.	PubMed
Infection	VACCINE-INDUCED PROTECTION AGAINST MURINE SCHISTOSOMIASIS MANSONI WITH LARVAL EXCRETORY-SECRETORY ANTIGENS AND PAPAIN OR TYPE-2 CYTOKINES.	PubMed
Infertility	Approaches to post-testicular contraception.	PubMed
Infertility, Male	Genome-wide profiling of gene expression in the epididymis of alpha-chlorohydrin-induced infertile rats using an oligonucleotide microarray.	PubMed
Infertility, Male	Glyceraldehyde 3-phosphate dehydrogenase-S, a sperm-specific glycolytic enzyme, is required for sperm motility and male fertility.	PubMed
Infertility, Male	The plastidial glyceraldehyde-3-phosphate dehydrogenase is critical for viable pollen development in Arabidopsis.	PubMed
Inflammatory Bowel Diseases	5-aminosalicylic acid prevents oxidant mediated damage of glyceraldehyde-3-phosphate dehydrogenase in colon epithelial cells.	PubMed
Influenza, Human	On the mechanism of phenotypic conversion of human cervical adenocarcinoma HeLa cells surviving infection by influenza B virus: Potential implications for biological management of adenocarcinomas.	PubMed
Insulin Resistance	Submicromolar concentrations of palmitoyl-CoA specifically thioesterify cysteine 244 in glyceraldehyde-3-phosphate dehydrogenase inhibiting enzyme activity: a novel mechanism potentially underlying fatty acid induced insulin resistance.	PubMed
Intellectual Disability	A New 5' Terminal Murine GAPDH Exon Identified Using 5'RACE LaNe.	PubMed
Intestinal Volvulus	Cloning, characterization and DNA immunization of an Onchocerca volvulus glyceraldehyde-3-phosphate dehydrogenase (Ov-GAPDH).	PubMed
Leukemia	Characterization of an immortalized oviduct cell line from the cynomolgus monkey (Macaca fascicularis).	PubMed
Leukemia	Structure/function in neuroprotection and apoptosis.	PubMed
Leukemia, Myelogenous, Chronic, BCR-ABL Positive	Molecular characterization of tumor associated glyceraldehyde-3-phosphate dehydrogenase.	PubMed
Liver Cirrhosis	Down-regulation of hepatic and renal 11 beta-hydroxysteroid dehydrogenase in rats with liver cirrhosis.	PubMed
Liver Cirrhosis	High serum glyceraldehyde-3-phosphate dehydrogenase levels in patients with liver cirrhosis.	PubMed
Lung Neoplasms	Choice of endogenous control for gene expression in nonsmall cell lung cancer.	PubMed
Lung Neoplasms	Enhanced expression of a glyceraldehyde-3-phosphate dehydrogenase gene in human lung cancers.	PubMed
Lung Neoplasms	Similarity between glyceraldehyde-3-phosphate dehydrogenase and a 37,000-dalton protein which is abundantly expressed in human lung cancers.	PubMed
Lyme Disease	A glyceraldehyde-3-phosphate dehydrogenase homolog in Borrelia burgdorferi and Borrelia hermsii.	PubMed
Lymphoma	Molecular characterization of BCL6 breakpoints in primary diffuse large B-cell lymphomas of the central nervous system identifies GAPD as novel translocation partner.	PubMed
Macular Degeneration	Studies on the mechanism of early onset macular degeneration in cynomolgus (Macaca fascicularis) monkeys. I. Abnormal concentrations of two proteins in the retina.	PubMed
Macular Degeneration	Studies on the mechanism of early onset macular degeneration in cynomolgus monkeys. II. Suppression of metallothionein synthesis in the retina in oxidative stress.	PubMed
Malaria	Crystal structure of glyceraldehyde-3-phosphate dehydrogenase from Plasmodium falciparum at 2.25 A resolution reveals intriguing extra electron density in the active site.	PubMed
Mastitis	Characterization of two proteins of Staphylococcus aureus isolated from bovine clinical mastitis with homology to glyceraldehyde-3-phosphate dehydrogenase.	PubMed
Melanoma	Gene expression profiling of melanocytic lesions.	PubMed
Melanoma	Glyceraldehyde-3-phosphate dehydrogenase expression is altered by hypoxia in melanoma cells and primary human melanocytes.	PubMed
Melanoma	Ribosomal 18S RNA prevails over glyceraldehyde-3-phosphate dehydrogenase and beta-actin genes as internal standard for quantitative comparison of mRNA levels in invasive and noninvasive human melanoma cell subpopulations.	PubMed
Melanoma	Sensitivity to extrinsically supplied interferon and the endogenous expression of interferon in melanoma cell lines.	PubMed
Melanoma	The normalization of gene expression data in melanoma: investigating the use of glyceraldehyde 3-phosphate dehydrogenase and 18S ribosomal RNA as internal reference genes for quantitative real-time PCR.	PubMed
Microphthalmos	Brachyury-related transcription factor Tbx2 and repression of the melanocyte-specific TRP-1 promoter.	PubMed
Motor Neuron Disease	Effects of gene therapy on muscle 18S rRNA expression in mouse model of ALS.	PubMed
Multiple Sclerosis	Inhibition of glyceraldehyde-3-phosphate dehydrogenase activity by antibodies present in the cerebrospinal fluid of patients with multiple sclerosis.	PubMed
Muscular Atrophy	Proteomic analysis of muscle affected by motor neuron degeneration: The wobbler mouse model of amyotrophic lateral sclerosis.	PubMed
Muscular Diseases	Metabolic myopathy produced by acute inhibition of glyceraldehyde-3-phosphate dehydrogenase with ortho-iodosobenzoic acid.	PubMed
Muscular Disorders, Atrophic	Spinocerebellar ataxia type-1 and spinobulbar muscular atrophy gene products interact with glyceraldehyde-3-phosphate dehydrogenase.	PubMed
Myoclonic Epilepsies, Progressive	Glyceraldehyde 3-phosphate dehydrogenase and endothelin-1 immunoreactivity is associated with cerebral white matter damage in dentatorubral-pallidoluysian atrophy.	PubMed
Myoclonic Epilepsies, Progressive	Tissue transglutaminase-catalyzed formation of high-molecular-weight aggregates in vitro is favored with long polyglutamine domains: a possible mechanism contributing to CAG-triplet diseases.	PubMed
Nasal Polyps	Expression of neutrophil gelatinase-associated lipocalin in nasal polyps.	PubMed
Neoplasm Metastasis	Phosphoglycerate mutase-derived polypeptide inhibits glycolytic flux and induces cell growth arrest in tumor cell lines.	PubMed
Neoplasms	A novel gene signature for molecular diagnosis of human prostate cancer by RT-qPCR.	PubMed
Neoplasms	A reverse transcription-quantitative competitive polymerase chain reaction (RT-qcPCR) technique to measure cytokine gene expression in domestic mammals.	PubMed
Neoplasms	Antisense oligodeoxynucleotide of glyceraldehyde-3-phosphate dehydrogenase gene inhibits cell proliferation and induces apoptosis in human cervical carcinoma cell lines.	PubMed
Neoplasms	Cancer abolishes the tissue type-specific differences in the phenotype of energetic metabolism.	PubMed
Neoplasms	Comparative proteomic analysis of esophageal squamous cell carcinoma.	PubMed
Neoplasms	Correlative histochemistry of some enzymes of carbohydrate metabolism in preneoplastic and neoplastic lesions in the rat liver.	PubMed
Neoplasms	Cytokine mRNA quantification in duodenal mucosa from dogs with chronic enteropathies by real-time reverse transcriptase polymerase chain reaction.	PubMed
Neoplasms	Cytokine profiles in eye muscle tissue and orbital fat tissue from patients with thyroid-associated ophthalmopathy.	PubMed
Neoplasms	Decreased expression of cytochromes P450 1A2, 2E1, and 3A4 and drug transporters Na+-taurocholate-cotransporting polypeptide, organic cation transporter 1, and organic anion-transporting peptide-C correlates with the progression of liver fibrosis in chronic hepatitis C patients.	PubMed
Neoplasms	Detection and quantitation of circulating cancer cells in the peripheral blood of lung cancer patients.	PubMed
Neoplasms	Development and validation of cytokine quantitative, real time RT-PCR assays for characterization of Asian elephant immune responses.	PubMed
Neoplasms	Development of a microarray for studying porcine cytokine production in blood mononuclear cells and intestinal biopsies.	PubMed
Neoplasms	Differential display and integrin alpha 6 messenger RNA overexpression in hepatocellular carcinoma.	PubMed
Neoplasms	Enhanced gene expression in breast cancer cells in vitro and tumors in vivo.	PubMed
Neoplasms	Enhancement of hammerhead ribozyme catalysis by glyceraldehyde-3-phosphate dehydrogenase.	PubMed
Neoplasms	Enzyme activities at the surface of intact Ehrlich tumor cells with albumin in the isotonic assay medium.	PubMed
Neoplasms	Expression of estrogen receptor (ER) subtypes and ERbeta isoforms in colon cancer.	PubMed
Neoplasms	Expression of the pituitary transcription factor Ptx-1, but not that of the trans-activating factor prop-1, is reduced in human corticotroph adenomas and is associated with decreased alpha-subunit secretion.	PubMed
Neoplasms	Expression of the smg p25A (a ras p21-like GTP-binding protein) gene in human neuroblastoma cell lines and tumor tissues.	PubMed
Neoplasms	Functional and molecular characterization of glioblastoma multiforme-derived cancer stem cells.	PubMed
Neoplasms	Gene expression profiles in thyroid carcinomas.	PubMed
Neoplasms	Genes of glycolysis are ubiquitously overexpressed in 24 cancer classes.	PubMed
Neoplasms	Glyceraldehyde 3-phosphate dehydrogenase depletion induces cell cycle arrest and resistance to antimetabolites in human carcinoma cell lines.	PubMed
Neoplasms	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) induces cancer cell senescence by interacting with telomerase RNA component.	PubMed
Neoplasms	Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) is pyruvylated during 3-bromopyruvate mediated cancer cell death.	PubMed
Neoplasms	Glyceraldehyde-3-phosphate dehydrogenase and other enzymatic activity in normal mouse lung and in lung tumors.	PubMed
Neoplasms	Glyceraldehyde-3-phosphate dehydrogenase binds to the AU-Rich 3' untranslated region of colony-stimulating factor-1 (CSF-1) messenger RNA in human ovarian cancer cells: possible role in CSF-1 posttranscriptional regulation and tumor phenotype.	PubMed
Neoplasms	Glyceraldehyde-3-phosphate dehydrogenase is a microtubule binding protein in a human colon tumor cell line.	PubMed
Neoplasms	Glyceraldehyde-3-Phosphate Dehydrogenase: A Promising Target for Molecular Therapy in Hepatocellular Carcinoma.	PubMed
Neoplasms	Glyceraldehyde-3-phosphate, a glycolytic intermediate, plays a key role in controlling cell fate via inhibition of caspase activity.	PubMed
Neoplasms	HDM2-binding partners: interaction with translation elongation factor EF1alpha.	PubMed
Neoplasms	High bad and bcl-xL gene expression and combined bad, bcl-xL, bax and bcl-2 mRNA levels: molecular predictors for survival of stage 2 soft tissue sarcoma patients.	PubMed
Neoplasms	High level of ezrin mRNA expression in an osteosarcoma biopsy sample with lung metastasis.	PubMed
Neoplasms	Human hepatocellular carcinoma in a mouse model: assessment of tumor response to percutaneous ablation by using glyceraldehyde-3-phosphate dehydrogenase antagonists.	PubMed
Neoplasms	Identification of an additional hypoxia responsive element in the glyceraldehyde-3-phosphate dehydrogenase gene promoter.	PubMed
Neoplasms	Identification of underexpressed genes in early- and late-stage primary ovarian tumors by suppression subtraction hybridization.	PubMed
Neoplasms	Inactivation of glyceraldehyde-3-phosphate dehydrogenase of human malignant cells by methylglyoxal.	PubMed
Neoplasms	Increased glyceraldehyde-3-phosphate dehydrogenase gene expression in human cervical cancers.	PubMed
Neoplasms	Interaction of calcyclin and its cyanogen bromide fragments with annexin II and glyceraldehyde 3-phosphate dehydrogenase.	PubMed
Neoplasms	Involvement of glyceraldehyde-3-phosphate dehydrogenase in tumor necrosis factor-related apoptosis-inducing ligand-mediated death of thyroid cancer cells.	PubMed
Neoplasms	Isolation and characterization of complementary DNA clones corresponding to genes induced in mouse epidermis in vivo by tumor promoters.	PubMed
Neoplasms	Modulation of caspase-independent cell death leads to resensitization of imatinib mesylate-resistant cells.	PubMed
Neoplasms	Modulatory effect of mycobacterium cell wall extract (Regressin) on lymphocyte blastogenic activity and macrophage cytokine gene transcription in swine.	PubMed
Neoplasms	Molecular characterization of tumor associated glyceraldehyde-3-phosphate dehydrogenase.	PubMed
Neoplasms	Molecular diagnosis of sentinel lymph node metastases in cervical cancer using squamous cell carcinoma antigen.	PubMed
Neoplasms	Novel insight into the role of GAPDH playing in tumor.	PubMed
Neoplasms	Overexpression of osteopontin in hepatocellular carcinoma.	PubMed
Neoplasms	Partial volume rat lung irradiation: Temporal fluctuations of in-field and out-of-field DNA damage and inflammatory cytokines following irradiation.	PubMed
Neoplasms	Persistence of multiple tumor-specific T-cell clones is associated with complete tumor regression in a melanoma patient receiving adoptive cell transfer therapy.	PubMed
Neoplasms	Phosphoglycerate mutase-derived polypeptide inhibits glycolytic flux and induces cell growth arrest in tumor cell lines.	PubMed
Neoplasms	Potassium channel-blockers as therapeutic agents to interfere with bone resorption of periodontal disease.	PubMed
Neoplasms	Properties of the glyceraldehyde-3-phosphate dehydrogenase from Ehrlich ascites tumor cells.	PubMed
Neoplasms	Protein and gene expression of tumor-associated antigen RCAS1 in esophageal squamous cell carcinoma.	PubMed
Neoplasms	Quantitation of O6-methylguanine-DNA methyltransferase gene messenger RNA in gliomas by means of real-time RT-PCR and clinical response to nitrosoureas.	PubMed
Neoplasms	Quantitation of vascular endothelial growth factor mRNA levels in human breast tumors and metastatic lymph nodes.	PubMed
Neoplasms	Quantitative real-time RT-PCR measurement of cytokine mRNA expression in the skin of normal cats and cats with allergic skin disease.	PubMed
Neoplasms	Removing the cells from adult bone marrow derived stem cell therapy does not eliminate cardioprotection.	PubMed
Neoplasms	Retinoic acid receptors alpha, beta and gamma, and cellular retinol binding protein-I expression in breast fibrocystic disease and cancer.	PubMed
Neoplasms	Role of endogenous nitric oxide in TNF-alpha and IL-1beta generation in hepatic ischemia-repefusion.	PubMed
Neoplasms	Selection of candidate housekeeping controls in tomato plants using EST data.	PubMed
Neoplasms	Solubilization of the plasmin receptor from human carcinoma cells.	PubMed
Neoplasms	Telomerase RNA as a detection marker in the serum of breast cancer patients.	PubMed
Neoplasms	The prognostic value of tetranectin immunoreactivity and plasma tetranectin in patients with ovarian cancer.	PubMed
Neoplasms	Thioredoxin, a regulator of gene expression.	PubMed
Neoplasms	Treatment of inflammatory bowel disease in a rodent model with the intestinal growth factor glucagon-like peptide-2.	PubMed
Neoplasms	Two-stage hepatectomy with effective perioperative chemotherapy does not induce tumor growth or growth factor expression in liver metastases from colorectal cancer.	PubMed
Neoplasms	Upregulation of glyceraldehyde-3-phosphate dehydrogenase mRNA in the spleen of tumor-bearing mice.	PubMed
Neoplasms	[Proteomic analysis of morphine rabbit myocardium with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry]	PubMed
Nephritis, Hereditary	Expression of type IV c

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Acipenser sp.
Acipenser transmontanus
Antheraea mylitta
Apis mellifera
Arabidopsis thaliana
Ascaris suum
Bacillus cereus
Bacillus coagulans
Bacillus coagulans KU
Bacillus megaterium
Bacillus megaterium DSM319
Bacillus subtilis
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Bombus nevadensis
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Bos taurus
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Bradyrhizobium japonicum CB1809
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Canis lupus familiaris
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Escherichia coli
Euglena gracilis
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Glycine max
Haloarcula vallismortis
Hippoglossus sp.
Homo sapiens
Huso huso
Hypocrea koningii
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Lactobacillus crispatus
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Lactobacillus plantarum LA 318
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Leishmania mexicana
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Lentinus polychrous KPM3
Lobster
Mammalia
Marsilea quadrifolia
Meleagris gallopavo
Mesocricetus auratus
Mus musculus
Neisseria meningitidis
Neisseria meningitidis Z2491
Oplegnathus fasciatus
Oryctolagus cuniculus
Palinurus vulgaris
Panulirus versicolor
Paracoccidioides brasiliensis
Phenylobacterium immobile E
Pinus sylvestris
Pisum sativum
Plasmodium brasilianum
Plasmodium brasilianum Peru II
Plasmodium coatneyi
Plasmodium cynomolgi
Plasmodium cynomolgi Berok
Plasmodium falciparum
Plasmodium falciparum FVO
Plasmodium fragile
Plasmodium fragile Nilgiri
Plasmodium knowlesi strain H
Plasmodium malariae
Plasmodium malariae Uganda I
Plasmodium reichenowi
Plasmodium reichenowi CDC1
Plasmodium vivax
Plasmodium vivax Salvador
Pleurodeles waltl
Pseudomonas aeruginosa
Pseudomonas sp.
Pseudomonas sp. pi9
Psithyrus suckleyi
Rattus norvegicus
Saccharomyces cerevisiae
Sardina pilchardus
Schistosoma mansoni
Sinapis alba
Solanum tuberosum
Spinacia oleracea
Staphylococcus aureus
Staphylococcus aureus MRSA252
Starmerella bombicola
Streptococcus equinus
Streptococcus oralis
Streptococcus pneumoniae
Streptococcus pyogenes
Streptomyces arenae
Sus scrofa
Tetrahymena pyriformis
Thermotoga maritima
Thermus aquaticus
Thermus thermophilus
Thermus thermophilus HB 8
Trypanosoma brucei
Trypanosoma cruzi
Vigna radiata var. radiata
Xanthomonas campestris
Xenopus laevis
Zymomonas mobilis