Information on EC 1.14.13.39 - nitric-oxide synthase:
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EC NUMBERCOMMENTARY
1.14.13.39-

RECOMMENDED NAMEGeneOntology No.
nitric-oxide synthaseGO:0051767

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+
show the reaction diagram		The enzyme in brain, but not that induced in lung or liver by endotoxin, requires Ca2+. The stoichiometry is not clear, but may involve a two-electron and a one-electron oxidation step---
L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+
show the reaction diagram		cytochrome P-450 enzymeMus musculus, Rattus norvegicus-440192
L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+
show the reaction diagram		cytochrome P-450 enzyme; possible mechanismMus musculus, Rattus norvegicus-440198
L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+
show the reaction diagram		alignment of heme-binding domain amino acid sequences of NOSMus musculus-440217
L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+
show the reaction diagram		dimerization is required, activation of NO-synthesis by enabling electron transfer between the reductase and the oxygenase domains, isolated monomers are inactiveMus musculus-440221
L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+
show the reaction diagram		structure-function study of macrophage enzymeMus musculus-440223
L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+
show the reaction diagram		subunits align in head-to-head manner with oxygenase domains interacting to form a dimer and reductase domains existing as independent extensionsMus musculus-440225
L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+
show the reaction diagram		isoform I and II are regulated by Ca2+/calmodulin, isoform II is Ca2+-independent, but requires calmodulin, inducible by cytokinesBos taurus, Homo sapiens, Mus musculus, Rattus norvegicus, Sus scrofa-440226
L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+
show the reaction diagram		relation between structure, function and binding of prosthetic groups during dissociation, unfolding and renaturationMus musculus-440238
L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+
show the reaction diagram		analysis of catalytic site by Fourier transform infrared spectroscopy, extent of modulation of vibrational modes upon photolysis of CO compoundBos taurus-658116
L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+
show the reaction diagram		analysis of transition states via stopped-flow spectroscopyBacillus subtilis-659336
L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+
show the reaction diagram		analysis of transition states via stopped-flow spectroscopyMus musculusP29477659336
L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+
show the reaction diagram		the key structural features that are involved in the substrate and active site interaction are highly conserved, e.g. residues Pro214, Glu235, Trp234, Tyr237, Asn246, and Gln129, substrate binding structure and mechanism, overviewBacillus anthracis-671709
L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+
show the reaction diagram		reaction mechanism, the reaction of the enzyme with oxygen is fast and takes place within several steps, separated by ephemeral intermediates, two short-lived oxy-compounds OxyI and OxyIIHomo sapiens, Rattus norvegicus-671728
L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+
show the reaction diagram		reaction mechanism, NO release and transport, overview, NO formation through sequential electron transfer and the oxidation of L-arginine, in the catalytic process, FMN, FAD, and tetrahydrobiopterin are required as coenzymes, and the presence of Ca2+/calmodulin can aid the electron transfer, isozymes NOS1 and NOS3 perform a similar reaction mechanismHomo sapiens-673804
L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+
show the reaction diagram		interaction and electrontransfer between enzyme domains and bound cofactors calmodulin, FMN, FAD, tetrahydrobiopterin, heme, and NADPH, overviewHomo sapiensP29474674558
L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+
show the reaction diagram		interaction and electrontransfer between enzyme domains and bound cofactors calmodulin, FMN, FAD, tetrahydrobiopterin, heme, and NADPH, overviewMus musculus-674558
L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+
show the reaction diagram		interaction and electrontransfer between enzyme domains and bound cofactors calmodulin, FMN, FAD, tetrahydrobiopterin, heme, and NADPH, overviewRattus norvegicusP29476674558
L-arginine + n NADPH + n H+ + m O2 = citrulline + nitric oxide + n NADP+
show the reaction diagram		reaction and kinetic mechanismBacillus subtilis, Staphylococcus aureus-687572

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
oxidation----
redox reaction----
reduction----

PATHWAYKEGG LinkMetaCyc Link
citrulline-nitric oxide cycle-PWY-4983
nitric oxide biosynthesis (plants)-PWY-6845

SYSTEMATIC NAMEIUBMB Comments
L-arginine,NADPH:oxygen oxidoreductase (nitric-oxide-forming)The enzyme in brain, but not that induced in lung or liver by endotoxin, requires Ca2+. The stoichiometry is not clear, but may involve a two-electron and a one-electron oxidation step.

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
bacterial nitric oxide synthaseBacillus subtilis, Staphylococcus aureus--684957
bacterial nitric-oxide synthaseBacillus subtilis--687579, 687703
bacterial nitric-oxide synthaseBacillus anthracis--687703
bNOSBacillus subtilis--684957, 687579, 687703
bNOSStaphylococcus aureus--684957
bNOSBacillus anthracis--687703
cb-NOSHomo sapiens, Rattus norvegicus, Sus scrofa-isoform I, constitutive, from brain440226
cytokine inducible NOSHomo sapiens--698461
DNOSDrosophila melanogaster--657741, 685094, 685095
e-NOSHomo sapiens-endothelial isoform III440236
e-NOSBos taurus-endothelial isoform III440240
EC-NOSBos taurus, Homo sapiens, Mus musculus-isoform III, in endothelial cells440226
endothelial nitric oxide synthaseHomo sapiens--671728, 673002, 673804, 674558, 697688, 697713, 699457, 700937
endothelial nitric oxide synthaseRattus norvegicus--671728
endothelial nitric oxide synthaseRattus norvegicus-isoform695396
endothelial nitric oxide synthaseBos taurus--696280
endothelial nitric oxide synthaseMus musculus--701000, 701194
endothelial nitric-oxide synthaseHomo sapiens--674451
endothelial nitric-oxide synthaseRattus norvegicus--687615
endothelial nitric-oxide synthaseBos taurus--687734, 698884
endothelial NO synthaseHomo sapiens--697713
endothelial NOSRattus norvegicus--685867
endothelial NOSOreochromis mossambicus x Oreochromis niloticus--687358
endothelial NOSBos taurus--687734
endothelial NOSMus musculus--688600
endothelial NOSHomo sapiens-isoform696110
endothelial NOSHomo sapiens--698461
endothelium-derived relaxation factor-forming enzyme----
endothelium-derived relaxing factor synthase----
eNOSHomo sapiens--671728, 673002, 674451, 674558, 696110, 697688, 697713, 698461, 699457, 700937
eNOSRattus norvegicus--671728, 685867, 687615
eNOSOreochromis mossambicus x Oreochromis niloticus--687358
eNOSBos taurus--687734, 696280, 698884
eNOSMus musculus--688600, 701000, 701194
eNOSRattus norvegicus-isoform695396
i-NOSMus musculus-isoform II, inducible440226, 440236, 440238
i-NOSHomo sapiens, Rattus norvegicus-isoform II, inducible440226, 440236
i-NOSRattus norvegicus-isoform698343
inducible nitric oxide synthaseRattus norvegicus--671728, 677252, 685867, 696643, 697765, 698484
inducible nitric oxide synthaseHomo sapiens--671728, 699439
inducible nitric oxide synthaseMus musculus--672016, 674558
inducible nitric oxide synthaseBos taurusQ9BDQ7-690183
inducible nitric oxide synthaseRattus norvegicus-isoform695396
inducible nitric-oxide synthaseMus musculus--684317, 687548, 688600, 698939
inducible NO synthaseRattus norvegicus--685867, 695853
inducible NO synthaseHomo sapiens--700432, 700490
inducible NOSMus musculus--687291, 688600
inducible NOSOreochromis mossambicus x Oreochromis niloticus--687358
inducible NOSHomo sapiens-isoform696110
inducible NOSRattus norvegicus-isoform698343
iNOSNicotiana tabacum--658405
iNOSRattus norvegicus--671728, 677252, 685867, 695853, 696643, 697765, 698484
iNOSHomo sapiens--671728, 696110, 698461, 699439, 700432
iNOSMus musculus--672016, 674558, 684317, 687291, 687548, 688600, 698939
iNOSOreochromis mossambicus x Oreochromis niloticus--687358
iNOSBos taurusQ9BDQ7-690183
iNOSRattus norvegicus-isoform695396
iNOSHomo sapiens-isoform700490
mitochondrial NO synthaseHomo sapiens--700432
mitochondrial-specific nitric oxide synthaseRattus norvegicus--699997
mtNOSMus musculus--658793
mtNOSRattus norvegicus--671278, 699997
mtNOSHomo sapiens--700432
n-NOSHomo sapiens, Rattus norvegicus-isoform I, neuronal enzyme440226, 440236
n-NOSSus scrofa-isoform I, neuronal enzyme440226
n-NOSRattus norvegicus-isoform698343
NADPH-diaphorase----
neuronal nitric oxide synthaseRattus norvegicus--671728, 672363, 674558, 685120, 686293, 697765, 699996, 700433, 711646
neuronal nitric oxide synthaseHomo sapiens--671728, 673680, 673804, 699457, 711779
neuronal nitric oxide synthaseCricetulus griseus--675520
neuronal nitric oxide synthaseMus musculus--686293
neuronal nitric-oxide synthaseRattus norvegicus--687615, 698852
neuronal nitric-oxide synthaseBos taurus--687734
neuronal NO synthaseHomo sapiens--660662, 700432, 700490
neuronal NO synthaseRattus norvegicus--695853
neuronal NOSOreochromis mossambicus x Oreochromis niloticus--687358
neuronal NOSRattus norvegicus--687697, 697042
neuronal NOSBos taurus--687734
neuronal NOSHomo sapiens-isoform696110
neuronal NOSRattus norvegicus-isoform698343
neuronal NOSHomo sapiens--698461
nitric oxid synthaseTriatoma infestans--686010
nitric oxide synthase----
nitric oxide synthaseRattus norvegicus--671278, 672524, 675257, 677252
nitric oxide synthaseLeishmania amazonensis--671631
nitric oxide synthaseBacillus anthracis--671709
nitric oxide synthaseMus musculus--672524, 673662, 687291
nitric oxide synthaseHomo sapiens--672524
nitric oxide synthaseRana pipiens--672844
nitric oxide synthaseGanoderma applanatum--673971
nitric oxide synthaseDrosophila melanogaster--685094
nitric oxide synthaseHomo sapiens--695513
nitric oxide synthase-like proteinBacillus anthracis--671709
nitric oxide synthetase----
nitric-oxide synthaseDrosophila melanogaster--685095
nitric-oxide synthaseMammalia--701646
nNOSRattus norvegicus--659257, 671728, 672363, 674558, 685120, 687615, 695853, 697042, 697765, 698852, 699996, 700433, 711646
nNOSHomo sapiens--660662, 671728, 673680, 696110, 698461, 699457, 700432, 711779
nNOSCricetulus griseus--675520
nNOSOreochromis mossambicus x Oreochromis niloticus--687358
nNOSBos taurus--687734
nNOSHomo sapiens-isoform700490
nNOSalphaMus musculus, Rattus norvegicus--686293
NO synthase----
NO synthaseRattus norvegicus--675257, 711646
NO synthase type IHomo sapiens-nNOS695513
NO synthase type IIHomo sapiens-iNOS695513
NO synthase type IIIHomo sapiens-eNOS695513
NOSLeishmania amazonensis--671631
NOSBacillus anthracis--671709
NOSMus musculus--672524, 673662, 687291
NOSRattus norvegicus--672524, 675257, 687697
NOSHomo sapiens--672524
NOSRana pipiens--672844
NOSGanoderma applanatum--673971
NOSTriatoma infestans--686010
NOSOreochromis mossambicus x Oreochromis niloticus--687358
NOSBacillus subtilis, Staphylococcus aureus--687572
NOS IRattus norvegicus--659257
NOS-2Homo sapiens--699439
NOS1Homo sapiens--673804
NOS1Rattus norvegicus-isozyme697412
NOS1Mammalia-isoform1701646
NOS2Mus musculus--687727, 693776
NOS2Mammalia-isoform2701646
NOS3Homo sapiens--673002, 673804, 674451
NOS3Rattus norvegicus-isozyme697412
NOS3Mammalia-isoform3701646
synthetase, nitric oxide----

CAS REGISTRY NUMBERCOMMENTARY
125978-95-2-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Aplysia californica-659646--Manually annotated by BRENDA team
Bacillus anthracis-687703--Manually annotated by BRENDA team
Bacillus anthracispathogenic bacterium responsible for causing anthrax671709--Manually annotated by BRENDA team
Bacillus subtilis-659336, 684957, 687572, 687579, 687703--Manually annotated by BRENDA team
Bos taurus-440203, 440214, 440226, 440227, 440229, 440235--Manually annotated by BRENDA team
Bos taurus-658116, 696280, 698884P29473UniProtManually annotated by BRENDA team
Bos taurus3 isoforms exist in bovine aortic endothelial cells differentiated by substrate specificity and Ca2+-dependence440204--Manually annotated by BRENDA team
Bos taurusendothelial isoform440240--Manually annotated by BRENDA team
Bos taurusfragment of iNOS690183Q9BDQ7UniProtManually annotated by BRENDA team
Bos taurusisozymes eNOS and nNOS687734--Manually annotated by BRENDA team
Cricetulus griseus-675520--Manually annotated by BRENDA team
Drosophila melanogaster-685094, 685095--Manually annotated by BRENDA team
Drosophila melanogasterexpression in Escherichia coli657741--Manually annotated by BRENDA team
Entamoeba histolytica-658664--Manually annotated by BRENDA team
Felis catusmongrel cat440224--Manually annotated by BRENDA team
Ganoderma applanatumshelf fungus, collected from forest in Darjeeling, East Sikkim, India673971--Manually annotated by BRENDA team
Homo sapiens-440194, 440205, 440215, 440216, 440218, 440226, 440233, 440236, 660371, 660662, 660685, 671728, 672524, 673002, 673680, 674451, 684277, 696110, 697713, 698461, 700432, 711779--Manually annotated by BRENDA team
Homo sapiens-695513, 696110, 699457, 700432, 700490P29475UniProtManually annotated by BRENDA team
Homo sapiens-695513, 696110, 699457, 700937P29474UniProtManually annotated by BRENDA team
Homo sapiens-695513, 699439, 700432, 700490P35228UniProtManually annotated by BRENDA team
Homo sapiens-697688Q62600UniProtManually annotated by BRENDA team
Homo sapiensexpression in Pichia pastoris660022--Manually annotated by BRENDA team
Homo sapiensinducible440219, 440237--Manually annotated by BRENDA team
Homo sapiensisozyme eNOS674558P29474UniProtManually annotated by BRENDA team
Homo sapiensisozymes NOS1 and NOS3673804--Manually annotated by BRENDA team
Homo sapiensneuronal enzyme, full-length enzyme, FAD-FMN-domain, FAD-NADPH-domain657670--Manually annotated by BRENDA team
Leishmania amazonensisconstitutive enzyme671631--Manually annotated by BRENDA team
Mammalia-701646--Manually annotated by BRENDA team
Mus musculus-440192, 440193, 440195, 440198, 440201, 440209, 440217, 440222, 440225, 440226, 440234, 440236, 658793, 672016, 687291, 687548--Manually annotated by BRENDA team
Mus musculus-658119, 660371, 693776, 698939P29477UniProtManually annotated by BRENDA team
Mus musculus-701000P70313UniProtManually annotated by BRENDA team
Mus musculus3 isoforms exist in mouse macrophages differentiated by substrate specificity and Ca2+-dependence440204--Manually annotated by BRENDA team
Mus musculusC57/BL 6J mice687727--Manually annotated by BRENDA team
Mus musculusC57BL/6 wild-type mice684317--Manually annotated by BRENDA team
Mus musculuscytokine-inducible in macrophage440200, 440206, 440221, 440223, 440238--Manually annotated by BRENDA team
Mus musculuscytokine-inducible in macrophage440219P29477UniProtManually annotated by BRENDA team
Mus musculusdifferent splice isoforms of nNOS, e.g. splicing variant nNOSalpha686293--Manually annotated by BRENDA team
Mus musculusinducible enzyme672524P29477UniProtManually annotated by BRENDA team
Mus musculusinducible oxygenase domain659336P29477UniProtManually annotated by BRENDA team
Mus musculusisozyme iNOS674558--Manually annotated by BRENDA team
Mus musculusisozyme NOS1673662--Manually annotated by BRENDA team
Mus musculusmale and female C57BL/6J mice688600--Manually annotated by BRENDA team
Mus musculusstrain C57BL/6440239--Manually annotated by BRENDA team
Mus musculustight-skin 1 mouse strain701194P70313UniProtManually annotated by BRENDA team
Nicotiana tabacum-658405--Manually annotated by BRENDA team
Oreochromis mossambicus x Oreochromis niloticushybrid tilapia, three isozymes eNOS, iNOS and nNOS687358--Manually annotated by BRENDA team
Oryctolagus cuniculus-661659--Manually annotated by BRENDA team
Ovis aries-440231--Manually annotated by BRENDA team
Rana pipiens-672844--Manually annotated by BRENDA team
Rattus norvegicus-440191, 440192, 440198, 440207, 440208, 440210, 440212, 440213, 440220, 440226, 658119, 659257, 659330, 671728, 672363, 685120, 687615, 697765, 699997--Manually annotated by BRENDA team
Rattus norvegicus-660371, 687697, 695853, 697042, 697412, 698343, 698852, 699996P29476UniProtManually annotated by BRENDA team
Rattus norvegicus-695396, 695853, 696643, 698343, 698484Q06518UniProtManually annotated by BRENDA team
Rattus norvegicus-695396, 697412Q62600UniProtManually annotated by BRENDA team
Rattus norvegicus-700433P29475UniProtManually annotated by BRENDA team
Rattus norvegicus2 isoforms; Sprague-Dawley440197--Manually annotated by BRENDA team
Rattus norvegicus2 isoforms; Wistar strain440190, 440202--Manually annotated by BRENDA team
Rattus norvegicusdifferent splice isoforms of nNOS, e.g. splicing variant nNOSalpha686293--Manually annotated by BRENDA team
Rattus norvegicusinducible enzyme672524--Manually annotated by BRENDA team
Rattus norvegicusisozyme nNOS674558P29476UniProtManually annotated by BRENDA team
Rattus norvegicusmale711646--Manually annotated by BRENDA team
Rattus norvegicusmale Sprague-Dawley rats, isozyme iNOS677252Q06518UniProtManually annotated by BRENDA team
Rattus norvegicusmale Sprague-Dawley rats, isozymes nNOS and iNOS675257--Manually annotated by BRENDA team
Rattus norvegicusneuronal enzyme659638P29476UniProtManually annotated by BRENDA team
Rattus norvegicusSprague-Dawley440196, 440203, 440228, 440230, 440236--Manually annotated by BRENDA team
Rattus norvegicusWistar and Wistar-Kyoto rats, and 1 month-old, prehypertensive/spontaneously hypertensive rats671278--Manually annotated by BRENDA team
Rattus norvegicusWistar rats685867--Manually annotated by BRENDA team
Rattus norvegicusWistar strain440232--Manually annotated by BRENDA team
Staphylococcus aureus-684957, 687572--Manually annotated by BRENDA team
Staphylococcus aureusinduced by methanol658767--Manually annotated by BRENDA team
Sus scrofa-440199, 440211, 440226--Manually annotated by BRENDA team
Triatoma infestans-686010--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
physiological functionRattus norvegicus-the brain neuronal NOS and inducible NOS are respectively involved in the bombesin-induced secretion of noradrenaline and adrenaline from the adrenal medulla697765
physiological functionHomo sapiens-eNOS selectively activates N-Ras but not K-Ras on the Golgi complex of T cells engaged with antigen-presenting cells by S-nitrosylation at Cys118700937
physiological functionMus musculusP70313si-RNA mediated knockdown of eNOS leads to a striking increase in AMP-activated protein kinase phosphorylation, homozygot eNOS knockout mice show a marked increase in AMP-activated protein kinase phosphorylation in liver and lung compared to wild type mice701000

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
1,4-bis-[[2-(dimethylamino-N-oxide)ethyl]amino]-5,8-dihydroxyanthracene-9,10-dione + NADPH1-[[2-(dimethylamino-N-oxide)ethyl]amino]-4-[[2-(dimethylamino)ethyl]amino]-5,8-dihydroxyanthracene-9,10-dione + ?
show the reaction diagram		Homo sapiens--699439--ir
1-butyl-2-hydroxyguanidine + NADPH + O2? + NO + NADP+
show the reaction diagram		Rattus norvegicusP29476-659638--?
1-[[2-(dimethylamino-N-oxide)ethyl]amino]-4-[[2-(dimethylamino)ethyl]amino]-5,8-dihydroxyanthracene-9,10-dione + NADPH1,4-bis[[2-(dimethylamino)ethyl]amino]-5,8-dihydroxyanthracene-9,10-dione + ?
show the reaction diagram		Homo sapiens--699439--ir
2,6-dichlorophenolindophenol + NADPH + O2? + NO + NADP+
show the reaction diagram		Rattus norvegicus--440210---
2,6-dichlorophenolindophenol + NADPH + O2? + NO + NADP+
show the reaction diagram		Sus scrofa--440199---
2,6-dichlorophenolindophenol + NADPH + O2? + NO + NADP+
show the reaction diagram		Drosophila melanogaster-best substrate, about 10-fold increase in activity in presence of calmodulin657741--?
2-hydroxy-1-(4-hydroxyphenyl)guanidine + NADPH + O2? + NO + NADP+
show the reaction diagram		Rattus norvegicusP29476-659638--?
2-hydroxy-1-isopropylguanidine + NADPH + O2? + NO + NADP+
show the reaction diagram		Rattus norvegicusP29476-659638--?
adriamycin + NADPH + O2? + NO + NADP+
show the reaction diagram		Homo sapiens--657670--?
ferricyanide + NADPH + O2ferrocyanide + NADP+ + H2O
show the reaction diagram		Bos taurus--440240--?
ferricyanide + NADPH + O2ferrocyanide + NO + NADP+
show the reaction diagram		Drosophila melanogaster--657741--?
ferricytochrome c + NADPH + O2ferrocytochrome c + NO + NADP+
show the reaction diagram		Drosophila melanogaster--657741--?
L-Ala-L-Arg + NADPH + O2?
show the reaction diagram		Mus musculus, Bos taurus-endothelial microsomes, macrophage440204--?
L-Arg-L-Arg + NADPH + O2?
show the reaction diagram		Mus musculus, Bos taurus-endothelial, microsomes, macrophage440204--?
L-Arg-L-Arg-L-Arg + NADPH + O2?
show the reaction diagram		Mus musculus, Bos taurus-endothelial microsomes440204--?
L-Arg-L-Phe + NADPH + O2?
show the reaction diagram		Mus musculus, Bos taurus--440204--?
L-arginine + H2O2 + tetrahydrobiopterin? + NO + NADP+
show the reaction diagram		Drosophila melanogaster--657741--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Staphylococcus aureus--684957, 687572--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Bacillus subtilis--684957, 687572, 687579--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Mus musculus--684317, 686293, 687291, 687548, 687727--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Homo sapiens--684277, 696110, 697713, 700432--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Rattus norvegicus--686293, 687615, 687697--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Bos taurus--687734--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Triatoma infestans--686010--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Bos taurusQ9BDQ7-690183--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Rattus norvegicus--695853, 696643, 698484--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Bos taurus--696280, 698884--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Rattus norvegicus--697412, 698852, 699996--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Homo sapiens--700490, 699457--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Rattus norvegicusP29475-700433--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Mus musculusP70313-701000, 701194--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Bacillus subtilis-overall reaction687579--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Homo sapiens-NO from acetylsalicylic acid-activated enzyme is involved in thrombolysis, overview684277--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Bos taurusQ9BDQ7NO is an important signalling molecule, released by numerous cells, that acts in many tissues to regulate a diverse range of physiological and biological processes, including neurotransmission, immune defence and the regulation of apoptosis. NO plays a major role in the killing of intracellular pathogens as part of the innate immune response690183--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Drosophila melanogaster-overall reaction, overview685094--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Drosophila melanogaster-regulatory mechanism, overview685095--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Rattus norvegicus-complete reaction, during Arg hydroxylation, H4B acts as a one-electron donor and is then presumed to redox cycle, i.e. be reduced back to H4B, within NOS before further catalysis can proceed. Calmodulin-dependent reduction of a tetrahydrobiopterin radical, mechanism involving the NOS flavoprotein domain, reaction scheme, overview687697--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Rattus norvegicus-complete reaction, oxygen stoichiometry, effects of substrate/cofactor binding on the endothelial NOS isoform, eNOS, overview687615--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Drosophila melanogaster-conserved residue Arg1329 of nNOS enables bound NADPH to stabilize the FMN-shielded conformation, overview685095--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Mus musculus-NOS catalyzes the formation of NO via a consecutive two-step reaction. In the first step, L-arginine is converted to N-hydroxy-L-arginine, in the second step, N-hydroxy-L-arginine is further converted to citrulline and nitric oxide, two different mechanisms, overview. During catalysis, mediated by calcium/calmodulin, electrons flow from NADPH through FAD and FMN in the reductase domain of one subunit of the homodimer to the oxygenase domain of the other subunit, substrate-ligand interaction in the Fe2+-O2 complex, overview687291--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Staphylococcus aureus, Bacillus subtilis-overall reaction, the interactions between heme-bound NO and the substrates are finely tuned by the geometry of the Fe-ligand structure, overview684957--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Staphylococcus aureus, Bacillus subtilis-overall reaction, two-step oxidation of L-arginine using an O2-dependent mechanism, detailed overview687572--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Mus musculus-substrate and product binding analysis687548--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Bos taurus-the electron transfer between cofactors FMN and FAD is reversible687734--?
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Rattus norvegicus-conversion of L-arginine to citrulline and nitric oxide takes place in two steps with N(G)-hydroxy-L-arginine as an intermediate product699997--?
L-arginine + NADPH + H+ + O2Nomega-hydroxy-L-arginine + NADP+ + H2O
show the reaction diagram		Staphylococcus aureus-first half reaction via intermediate Nomega-hydroxy-L-arginine684957, 687572--?
L-arginine + NADPH + H+ + O2Nomega-hydroxy-L-arginine + NADP+ + H2O
show the reaction diagram		Bacillus subtilis-first half reaction via intermediate Nomega-hydroxy-L-arginine684957, 687572, 687579--?
L-arginine + NADPH + H+ + O2Nomega-hydroxy-L-arginine + NADP+ + H2O
show the reaction diagram		Rattus norvegicus-first half reaction via intermediate Nomega-hydroxy-L-arginine687615, 687697--?
L-arginine + NADPH + H+ + O2Nomega-hydroxy-L-arginine + NADP+ + H2O
show the reaction diagram		Drosophila melanogaster-first half reaction via intermediate Nomega-hydroxy-L-arginine with consecutive appearance of heme-dioxy, ferric heme-NO, and ferric heme species, overview685094--?
L-arginine + NADPH + H+ + O2Nomega-hydroxy-L-arginine + NADP+ + H2O
show the reaction diagram		Homo sapiens-first half reaction698461--ir
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Staphylococcus aureus--658767--?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus--440193-440193?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus--440195-440195?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus--440200-440200?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus--440201-440201?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus--440204-440204?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus--440206-440206?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus--440209-440209?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus--440217-440217?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculusP29477-440219--?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus--440225-440225?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus--440226-440226?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus--440234-440234?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus--440236-440236?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus--440238-440238?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus--440239-440239?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Homo sapiens--440194-440194?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Homo sapiens--440205-440205?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Homo sapiens--440216-440216?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Homo sapiens--440218-440218?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Homo sapiens--440219-440219?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Homo sapiens--440226-440226?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Homo sapiens--440233-440233?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Homo sapiens--440236-440236?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Homo sapiens--440237-440237?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Homo sapiens--660022--?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Rattus norvegicus--440190-440190?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Rattus norvegicus--440191-440191?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Rattus norvegicus--440197-440197?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Rattus norvegicus--440202-440202?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Rattus norvegicus--440203-440203?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Rattus norvegicus--440207-440207?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Rattus norvegicus--440213-440213?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Rattus norvegicus--440226-440226?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Rattus norvegicus--440228-440228?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Rattus norvegicus--440230-440230?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Rattus norvegicus--440232-440232?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Rattus norvegicus--440236-440236?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Rattus norvegicus--659257--?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Rattus norvegicusP29476-659638--?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Sus scrofa--440211-440211?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Sus scrofa--440226-440226?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Bos taurus--440203-440203?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Bos taurus--440204-440204?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Bos taurus--440214-440214?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Bos taurus--440226-440226?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Bos taurus--440227-440227?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Bos taurus--440229-440229?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Bos taurus--440235-440235?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Bos taurus--440240-440240?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Ovis aries--440231-440231?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Nicotiana tabacum--658405--?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Entamoeba histolytica--658664--?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Felis catus--440224-440224?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Homo sapiens--440215the product is a guanylyl-cyclase-relaxing factor, that is identical with nitric oxide or a NO-releasing compound440215?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Rattus norvegicus--440196the product is a guanylyl-cyclase-relaxing factor, that is identical with nitric oxide or a NO-releasing compound440196?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Rattus norvegicus--440210the product is a guanylyl-cyclase-relaxing factor, that is identical with nitric oxide or a NO-releasing compound440210?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Sus scrofa--440199the product is a guanylyl-cyclase-relaxing factor, that is identical with nitric oxide or a NO-releasing compound440199?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus, Rattus norvegicus--440192probably via Nomega-hydroxy-L-arginine440192?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Rattus norvegicus--440208guanidino-nitrogen of L-arginine is oxidized to form NO and citrulline440208?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus-the overall reaction proceeds via 2 partial reactions: reaction 1 converts L-arginine into L-Ngamma-hydroxyarginine, reaction 2 converts L-Ngamma-hydroxyarginine into citrulline and nitric oxide440221-440221?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Rattus norvegicus-the overall reaction proceeds via 2 partial reactions: reaction 1 converts L-arginine into L-Ngamma-hydroxyarginine, reaction 2 converts L-Ngamma-hydroxyarginine into citrulline and nitric oxide440220-440220ir
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus-capacity to synthesize NO only through dimerization and binding of heme and tetrahydrobiopterin440223-440223?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculusP29477dimeric structure is required for enzyme activity440219-440219?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus-dimeric structure is required for enzyme activity440221-440221?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus-tetrahydrobiopterin is absolutely required for partial reaction 1440222-440222?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus, Rattus norvegicus-specific for NADPH, 5-electron oxidation of L-arginine440198-440198?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus, Homo sapiens, Rattus norvegicus, Sus scrofa, Bos taurus-physiological functions and pathophysiology of the isoforms440226--?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Homo sapiens-a cytokine-inducible, calcium independent and a constitutive, calcium dependent form440194-440194?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus-acts as signal molecule for neurotransmission, vasorelaxation, and cytotoxity440192, 440200--?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Rattus norvegicus-acts as signal molecule for neurotransmission, vasorelaxation, and cytotoxity440192--?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus-enzyme of mammalian immune, cardiovascular and neural systems, synthesizing the free radical nitric oxide or a NO-releasing product440201--?
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus, Rattus norvegicus-soluble cytochrome P-450 enzyme in eukaryotes440198--?
L-arginine + NADPH + O2L-citrulline + NO + NADP+ + H2O
show the reaction diagram		Cricetulus griseus--675520--?
L-arginine + NADPH + O2L-citrulline + NO + NADP+ + H2O
show the reaction diagram		Mus musculusP29477-672524--?
L-arginine + NADPH + O2L-citrulline + NO + NADP+ + H2O
show the reaction diagram		Mus musculus--673662, 674558--?
L-arginine + NADPH + O2L-citrulline + NO + NADP+ + H2O
show the reaction diagram		Homo sapiens--671728, 672524, 673804--?
L-arginine + NADPH + O2L-citrulline + NO + NADP+ + H2O
show the reaction diagram		Homo sapiensP29474-674558--?
L-arginine + NADPH + O2L-citrulline + NO + NADP+ + H2O
show the reaction diagram		Rattus norvegicus--671278, 671728, 672363, 672524--?
L-arginine + NADPH + O2L-citrulline + NO + NADP+ + H2O
show the reaction diagram		Rattus norvegicusP29476-674558--?
L-arginine + NADPH + O2L-citrulline + NO + NADP+ + H2O
show the reaction diagram		Rattus norvegicus--675257--?
L-arginine + NADPH + O2L-citrulline + NO + NADP+ + H2O
show the reaction diagram		Rattus norvegicusQ06518-677252--?
L-arginine + NADPH + O2L-citrulline + NO + NADP+ + H2O
show the reaction diagram		Leishmania amazonensis--671631--?
L-arginine + NADPH + O2L-citrulline + NO + NADP+ + H2O
show the reaction diagram		Bacillus anthracis--671709--?
L-arginine + NADPH + O2L-citrulline + NO + NADP+ + H2O
show the reaction diagram		Rattus norvegicus-mitochondrial nitric oxide production is involved in modulation of several organelle functions, such as transmembrane potential and matrix pH, inhibition of respiration by competitive inhibition with oxygen in cytochrome c oxidase, inhibition of ATP synthesis, permeability transition pore (PTP) opening, apoptosis and cell death, overview671278--?
L-arginine + NADPH + O2L-citrulline + NO + NADP+ + H2O
show the reaction diagram		Bacillus anthracis-the enzyme forms a five-coordinate, high-spin complex with L-arginine and analogues, e.g. N-hydroxy-L-arginine671709--?
L-arginine + NADPH + O2 + tetrahydrobiopterincitrulline + NO + NADP+ + ?
show the reaction diagram		Drosophila melanogaster--657741--?
L-arginine + NADPH + O2 + tetrahydrobiopterincitrulline + NO + NADP+ + ?
show the reaction diagram		Mus musculusP29477-658119--?
L-arginine + NADPH + O2 + tetrahydrobiopterincitrulline + NO + NADP+ + ?
show the reaction diagram		Rattus norvegicus--440196---
L-arginine + NADPH + O2 + tetrahydrobiopterincitrulline + NO + NADP+ + ?
show the reaction diagram		Rattus norvegicus--658119--?
L-homoarginine + NADPH + O2?
show the reaction diagram		Rattus norvegicus-poor substrate440190--?
L-homoarginine + NADPH + O2?
show the reaction diagram		Mus musculus, Bos taurus-constitutive endothelial membrane-bound and inducible soluble macrophage enzyme440204--?
menadione + NADPH + O2? + NO + NADP+
show the reaction diagram		Homo sapiens--657670--?
N-hydroxy-L-arginine + H2O2 + tetrahydrobiopterin? + NADP+
show the reaction diagram		Drosophila melanogaster--657741--?
N-hydroxy-L-arginine + NADPH + O2? + NO + NADP+
show the reaction diagram		Bacillus subtilis--659336--?
N-hydroxy-L-arginine + NADPH + O2? + NO + NADP+
show the reaction diagram		Mus musculusP29477-659336--?
N-hydroxy-L-arginine + NADPH + O2? + NO + NADP+
show the reaction diagram		Rattus norvegicus--659330--?
N-hydroxy-L-arginine + NADPH + O2? + NO + NADP+
show the reaction diagram		Rattus norvegicusP29476-659638--?
Ngamma-hydroxy-L-arginine + H2O2citrulline + Ndelta-cyanoornithine + NO2- + NO3-
show the reaction diagram		Mus musculus-tetrahydrobiopterin-free440222NO2-/NO3- as aerobic decomposition products from NO-440222?
Ngamma-hydroxy-L-arginine + NADPH + O2citrulline + NADP+ + NO
show the reaction diagram		Mus musculus--440193-440193?
Ngamma-hydroxy-L-arginine + NADPH + O2citrulline + NADP+ + NO
show the reaction diagram		Mus musculus--440223--?
Ngamma-hydroxy-L-arginine + NADPH + O2citrulline + NADP+ + NO
show the reaction diagram		Bos taurus--440240--?
Ngamma-hydroxy-L-arginine + NADPH + O2citrulline + NADP+ + NO
show the reaction diagram		Mus musculus, Bos taurus-best substrate440204--?
Ngamma-hydroxy-L-arginine + NADPH + O2citrulline + NADP+ + NO
show the reaction diagram		Mus musculus-substrate is intermediate between reaction 1 and 2 to form citrulline and NO from L-arginine440222-440222?
Ngamma-hydroxy-L-arginine + NADPH + O2citrulline + NADP+ + NO
show the reaction diagram		Rattus norvegicus-substrate is intermediate between reaction 1 and 2 to form citrulline and NO from L-arginine440220--ir
Ngamma-hydroxy-L-arginine + NADPH + O2citrulline + NADP+ + NO
show the reaction diagram		Mus musculus-reaction is possible without tetrahydrobiopterin, can also use H2O2 instead of NADPH and O2440222-440222?
nitroblue tetrazolium + NADPHnitroblue tetrazolium-flavazone + NADP+
show the reaction diagram		Sus scrofa--440199---
nitroblue tetrazolium + NADPHnitroblue tetrazolium-flavazone + NADP+
show the reaction diagram		Rattus norvegicus-NADPH-diaphorase reaction440196-440196?
Nomega-hydroxy-L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Drosophila melanogaster-second half reaction via intermediate Nomega-hydroxy-L-arginine685094---
Nomega-hydroxy-L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Staphylococcus aureus-second half reaction via intermediate Nomega-hydroxy-L-arginine687572---
Nomega-hydroxy-L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Bacillus subtilis-second half reaction via intermediate Nomega-hydroxy-L-arginine684957, 687572, 687579---
Nomega-hydroxy-L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Rattus norvegicus-second half reaction via intermediate Nomega-hydroxy-L-arginine687615, 687697---
Nomega-hydroxy-L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Staphylococcus aureus-second half reaction via intermediate Nomega-hydroxy-L-arginine, FeII and FeII-NO complexes bind Nomega-hydroxy-L-arginine, overview684957---
Nomega-hydroxy-L-arginine + NADPH + H+ + O2L-citrulline + NADP+ + NO + H2O
show the reaction diagram		Homo sapiens-second half reaction698461--?
oxidized cytochrome c + NADPH + O2reduced cytochrome c + NADP+ + H2O
show the reaction diagram		Mus musculusP29477-440219--?
oxidized cytochrome c + NADPH + O2reduced cytochrome c + NADP+ + H2O
show the reaction diagram		Mus musculus--440225--?
oxidized cytochrome c + NADPH + O2reduced cytochrome c + NADP+ + H2O
show the reaction diagram		Homo sapiens--440216, 440219--?
oxidized cytochrome c + NADPH + O2reduced cytochrome c + NADP+ + H2O
show the reaction diagram		Bos taurus--440240, 696280--?
oxidized cytochrome c + NADPH + O2reduced cytochrome c + NADP+ + H2O
show the reaction diagram		Mus musculus-wild-type and mutants440234--?
oxidized cytochrome c + NADPH + O2reduced cytochrome c + NADP+ + H2O
show the reaction diagram		Sus scrofa-reaction is enhanced by addition of calmodulin at 0.0002 mM440199--?
peroxynitrite + 4-hydroxyphenylacetic acid + NADPH + H+4-hydroxyl-3-nitro-phenylacetic acid + NADP+ + H2O
show the reaction diagram		Mus musculus-oxidation and nitration, although H4B binding seems unable to affect iNOSoxy capacity to activate peroxynitrite decomposition, the binding of Arg and citrulline at the distal side of the heme pocket drastically reduces peroxynitrite activation687548product dimers--
mitomycin c + NADPH + O2? + NO + NADP+
show the reaction diagram		Homo sapiens--657670--?
additional information?-Sus scrofa-the reductase domain has a broad substrate specificity, catalyzes a moderate Ca2+/calmodulin independent hydroxylation when the enzyme is reconstituted with purified P-450440199---
additional information?-Bos taurus-enzyme shows also superoxide formation activity, uneffected by L-arginine, inhibited by tetrahydrobiopterin and diphenyleneiodonium440229---
additional information?-Mus musculus-Ngamma-hydroxylation is the first step of the reaction, Ngamma-hydroxy-L-arginine being an intermediate in the L-arginine to NO pathway440193---
additional information?-Rattus norvegicus-the enzyme exhibits NADPH-diaphorase activity, uncoupled from nitric oxide synthase activity440196---
additional information?-Homo sapiens-enzyme can also Ca2+/calmodulin-dependently produce superoxide in absence of tetrahydropterin and in depletion of L-arginine, which is inhibited by tetrahydropterin, cyanide and imidazole440218---
additional information?-Mus musculus-dimeric enzyme and subunits are equivalent in catalyzing electron transfer from NADPH to cytochrome c, dichlorophenolindiphenol, and ferricyanide, D-arginine is no substrate440223---
additional information?-Rattus norvegicus-D-arginine is no substrate440190---
additional information?-Bos taurus-enzyme shows also superoxide formation activity440229---
additional information?-Homo sapiens-NO represents the endogenous activator of soluble guanylyl cyclase440215---
additional information?-Rattus norvegicus-NO represents the endogenous activator of soluble guanylyl cyclase440196, 440210---
additional information?-Sus scrofa-NO represents the endogenous activator of soluble guanylyl cyclase440199---
additional information?-Homo sapiens-neuronal NO synthase may be involved in the pathogenesis of acute lung injury after smoke inhalation injury followed by bacterial instillation in the airway660662---
additional information?-Homo sapiens-Pseudomonas aeruginosa stimulates expression of inducible nitric oxide synthase by A-549 cells. NO may be the mediator of epithelial damage caused by Pseudomonas aeruginosa660685---
additional information?-Mus musculus-calmodulin-controlled isoforms are signal generators, overview674558---
additional information?-Homo sapiensP29474calmodulin-controlled isoforms are signal generators, overview674558---
additional information?-Rattus norvegicusP29476calmodulin-controlled isoforms are signal generators, overview674558---
additional information?-Rattus norvegicus-caveolin-1 is a prominent NOS-interacting protein in rat polymorphonuclear neutrophils675257---
additional information?-Homo sapiens-cell-specific gene regulation mechanism of the endothelial isozyme in the vascular endothelium involving endothelial-specific promoter, binding sites for AP-1, high affinity Sp1-binding sites and GATA promoter sites, and several, e.g. octameric, transcriptional regulators, epigenetic regulatory mechanisms in vascular endothelial cell-specific gene expression, genetic, endothelial-specific regulation model, overview673002---
additional information?-Ganoderma applanatum-crude, boiled or ethanolic and dried extracts of Ganoderma applanatum show antioxidant activity, inhibition of lipid peroxidation, and potent hydroxylradical scavenging activity, overview673971---
additional information?-Homo sapiens-genetic regulation, mechanism, eNOS expression is controlled by both histone acetylation and lysine 4 methylation of histone H3 at eNOS proximal promoter regions, overview674451---
additional information?-Rattus norvegicusQ06518NO is implicated in the pathogenesis of liver cirrhosis, overview677252---
additional information?-Cricetulus griseus-postsynaptic density 95 proteins mediate the complex formation of neuronal nitric oxide synthase and N-methyl-D-aspartate receptors675520---
additional information?-Bacillus anthracis-the enzyme is involved in a multi-turnover process that results in NO as a product, NO is important in various pathological and physiological processes, NO produced by Bacillus anthracis may also have a pivotal pathophysiological role in anthrax infection671709---
additional information?-Leishmania amazonensis-the enzyme might be involved in the infectivity and/or escaping mechanism of the parasite671631---
additional information?-Mus musculus-both oxyFMN and oxygenase domain activity are measured by following H2O2-supported oxidation of Nomega-hydroxy-L-Arg, L-NOHA, overview674558---
additional information?-Homo sapiensP29474both oxyFMN and oxygenase domain activity are measured by following H2O2-supported oxidation of Nomega-hydroxy-L-Arg, L-NOHA, overview674558---
additional information?-Rattus norvegicusP29476both oxyFMN and oxygenase domain activity are measured by following H2O2-supported oxidation of Nomega-hydroxy-L-Arg, L-NOHA, overview674558---
additional information?-Homo sapiens-the enzyme interacts with Vac14, the activator of the PtdIns(3)P 5-kinase PIKfyve, the beta-finger independent interaction is based on an internal motif, sequence -G-D-H-L-D-, for PDZ recognition, PDZ domains are protein interaction modules found in single or multiple copies in a variety of proteins involved in multiprotein signaling complexes, interaction study with wild-type and mutant Vac14 proteins, binding is not abolished by deletion of the last five amino acids, but is abolished with deletions of the last 53 or last 10 residues of Vac14, overview673680---
additional information?-Drosophila melanogaster-dNOS participates in essential developmental and behavioral aspects of the fruit fly685095---
additional information?-Drosophila melanogaster-Drosophila dNOS is a more efficient and active NO synthase than the mammalian NOS enzymes, which may allow it to function more broadly in cell signaling and immune functions in the fruit fly685094---
additional information?-Rattus norvegicus-eNOS uncoupling is known to be controlled by substrate/cofactor availability, and the uncoupled reactions play important roles under various physiological/pathological conditions, such as atherosclerosis and septic shock687615---
additional information?-Rattus norvegicus-increased iNOS expression due to ethanol intake is responsible for gender differences in the vascular effects elicited by chronic ethanol consumption, while ovarian hormones do not play a role, overview685867---
additional information?-Mus musculus-inducible nitric-oxide synthase-derived NO contributes to the pathophysiology of intestinal inflammation in the colon684317---
additional information?-Mus musculus-iNOS modulates endothelin-1-dependent release of prostacyclin and inhibition of platelet aggregation ex vivo in the mouse, overview688600---
additional information?-Mus musculus-nitric-oxide synthase 2 interacts with CD74 and inhibits its cleavage by caspase during dendritic cell development687727---
additional information?-Bacillus subtilis, Bacillus anthracis-the bacterial enzyme, bNOS, lacks an essential reductase domain, that supplies electrons during NO biosynthesis, and is thus limited with respect to a pool of available redox partners, but does produce NO in living cells and accomplish this task by hijacking available cellular redox partners that are not normally committed to NO production, bacterial reductase also supports NO synthesis by the oxygenase domain of mammalian NOS expressed in Escherichia coli, overview687703---
additional information?-Mus musculus-the enzyme exclusively performs the nitric oxide synthesis, an essential biological mediator, and of peroxynitrite, a well known cytotoxic agent involved innumerouspathophysiological processes, NOSs have the unique ability to both produce and activate peroxynitrite, overview687548---
additional information?-Rattus norvegicus-three unique structural elements are involved in the catalytic suppression of NOS: an autoinhibitory element in the FMN binding module, a CD2A loop in the connecting subdomain, and a C-terminal extension or tail, the C-terminal tail of nNOS is a regulatory element that suppresses nNOS activities in the absence of bound calmodulin, it may help stabilize the FMN-shielded conformation by holding the FMN module up against the FNR module as required for inter-flavin electron transfer, mechanism, overview685120---
additional information?-Drosophila melanogaster-a oxygenase domain of dNOS complex with ferrous heme-NO is relatively unreactive toward O2685094---
additional information?-Bos taurus-endothelial NOS has a 6fold lower NO synthesis activity and 6-16fold lower cytochrome c reductase activity than neuronal NOS due to a significantly different electron transfer capacities, substrate specificity and mechanism, oveview687734---
additional information?-Mus musculus-interaction between peroxynitrite and the oxygenase domain of inducible NOS687548---
additional information?-Staphylococcus aureus, Bacillus subtilis-mechanisms of oxygen activation by NOSs, overview687572---
additional information?-Rattus norvegicus-nitric-oxide synthases are catalytically self-sufficient flavo-heme enzymes that generate NO from L-arginine and display an utilization of their tetrahydrobiopterin cofactor, overview687697---
additional information?-Bacillus subtilis-the bacterial NOS enzymes have no attached flavoprotein domain to reduce their heme and so must rely on unknown bacterial proteins for electrons687579---
additional information?-Rattus norvegicus-the reduced recombinant trunaction mutant nNOSr performs autooxidation in presence of NADPH, interactions, overview685120---
additional information?-Mus musculusP70313eNOS is an important negative regulator of AMP-activated protein kinase phosphorylation and intracellular H2O2 generation in endothelial cells701000---
additional information?-Homo sapiens-NOS has also nitrite reductase activity, the release of free nitric oxide from anoxic nitrite solutions at 0.015 mM is specific to the eNOS isoform and does not occur with the nNOS or iNOS isoforms696110---

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Staphylococcus aureus--684957, 687572--
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Bacillus subtilis--684957, 687572, 687579--
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Mus musculus--684317, 686293, 687291, 687548, 687727--
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Rattus norvegicus--686293, 687615, 687697--
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Bos taurus--687734--
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Triatoma infestans--686010--
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Homo sapiens-NO from acetylsalicylic acid-activated enzyme is involved in thrombolysis, overview684277--
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Bos taurusQ9BDQ7NO is an important signalling molecule, released by numerous cells, that acts in many tissues to regulate a diverse range of physiological and biological processes, including neurotransmission, immune defence and the regulation of apoptosis. NO plays a major role in the killing of intracellular pathogens as part of the innate immune response690183--
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Drosophila melanogaster-overall reaction, overview685094--
L-arginine + NADPH + H+ + O2citrulline + nitric oxide + NADP+ + H2O
show the reaction diagram		Drosophila melanogaster-regulatory mechanism, overview685095--
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculusP29477-440219--
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus--440239--
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Homo sapiens--440219--
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Rattus norvegicus--440190--
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Bos taurus--440229-440229
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus, Homo sapiens, Rattus norvegicus, Sus scrofa, Bos taurus-physiological functions and pathophysiology of the isoforms440226--
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Homo sapiens-a cytokine-inducible, calcium independent and a constitutive, calcium dependent form440194-440194
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus-acts as signal molecule for neurotransmission, vasorelaxation, and cytotoxity440192, 440200--
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Rattus norvegicus-acts as signal molecule for neurotransmission, vasorelaxation, and cytotoxity440192--
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus-enzyme of mammalian immune, cardiovascular and neural systems, synthesizing the free radical nitric oxide or a NO-releasing product440201--
L-arginine + NADPH + O2citrulline + NO + NADP+
show the reaction diagram		Mus musculus, Rattus norvegicus-soluble cytochrome P-450 enzyme in eukaryotes440198--
L-arginine + NADPH + O2L-citrulline + NO + NADP+ + H2O
show the reaction diagram		Cricetulus griseus--675520--
L-arginine + NADPH + O2L-citrulline + NO + NADP+ + H2O
show the reaction diagram		Mus musculusP29477-672524--
L-arginine + NADPH + O2L-citrulline + NO + NADP+ + H2O
show the reaction diagram		Mus musculus--673662, 674558--
L-arginine + NADPH + O2L-citrulline + NO + NADP+ + H2O
show the reaction diagram		Homo sapiens--671728, 672524, 673804--
L-arginine + NADPH + O2L-citrulline + NO + NADP+ + H2O
show the reaction diagram		Homo sapiensP29474-674558--
L-arginine + NADPH + O2L-citrulline + NO + NADP+ + H2O
show the reaction diagram		Rattus norvegicus--671728, 672363, 672524--
L-arginine + NADPH + O2L-citrulline + NO + NADP+ + H2O
show the reaction diagram		Rattus norvegicusP29476-674558--
L-arginine + NADPH + O2L-citrulline + NO + NADP+ + H2O
show the reaction diagram		Rattus norvegicus--675257--
L-arginine + NADPH + O2L-citrulline + NO + NADP+ + H2O
show the reaction diagram		Rattus norvegicusQ06518-677252--
L-arginine + NADPH + O2L-citrulline + NO + NADP+ + H2O
show the reaction diagram		Leishmania amazonensis--671631--
L-arginine + NADPH + O2L-citrulline + NO + NADP+ + H2O
show the reaction diagram		Bacillus anthracis--671709--
L-arginine + NADPH + O2L-citrulline + NO + NADP+ + H2O
show the reaction diagram		Rattus norvegicus-mitochondrial nitric oxide production is involved in modulation of several organelle functions, such as transmembrane potential and matrix pH, inhibition of respiration by competitive inhibition with oxygen in cytochrome c oxidase, inhibition of ATP synthesis, permeability transition pore (PTP) opening, apoptosis and cell death, overview671278--
additional information?-Bos taurus-enzyme shows also superoxide formation activity440229--
additional information?-Homo sapiens-NO represents the endogenous activator of soluble guanylyl cyclase440215--
additional information?-Rattus norvegicus-NO represents the endogenous activator of soluble guanylyl cyclase440196, 440210--
additional information?-Sus scrofa-NO represents the endogenous activator of soluble guanylyl cyclase440199--
additional information?-Homo sapiens-neuronal NO synthase may be involved in the pathogenesis of acute lung injury after smoke inhalation injury followed by bacterial instillation in the airway660662--
additional information?-Homo sapiens-Pseudomonas aeruginosa stimulates expression of inducible nitric oxide synthase by A-549 cells. NO may be the mediator of epithelial damage caused by Pseudomonas aeruginosa660685--
additional information?-Mus musculus-calmodulin-controlled isoforms are signal generators, overview674558--
additional information?-Homo sapiensP29474calmodulin-controlled isoforms are signal generators, overview674558--
additional information?-Rattus norvegicusP29476calmodulin-controlled isoforms are signal generators, overview674558--
additional information?-Rattus norvegicus-caveolin-1 is a prominent NOS-interacting protein in rat polymorphonuclear neutrophils675257--
additional information?-Homo sapiens-cell-specific gene regulation mechanism of the endothelial isozyme in the vascular endothelium involving endothelial-specific promoter, binding sites for AP-1, high affinity Sp1-binding sites and GATA promoter sites, and several, e.g. octameric, transcriptional regulators, epigenetic regulatory mechanisms in vascular endothelial cell-specific gene expression, genetic, endothelial-specific regulation model, overview673002--
additional information?-Ganoderma applanatum-crude, boiled or ethanolic and dried extracts of Ganoderma applanatum show antioxidant activity, inhibition of lipid peroxidation, and potent hydroxylradical scavenging activity, overview673971--
additional information?-Homo sapiens-genetic regulation, mechanism, eNOS expression is controlled by both histone acetylation and lysine 4 methylation of histone H3 at eNOS proximal promoter regions, overview674451--
additional information?-Rattus norvegicusQ06518NO is implicated in the pathogenesis of liver cirrhosis, overview677252--
additional information?-Cricetulus griseus-postsynaptic density 95 proteins mediate the complex formation of neuronal nitric oxide synthase and N-methyl-D-aspartate receptors675520--
additional information?-Bacillus anthracis-the enzyme is involved in a multi-turnover process that results in NO as a product, NO is important in various pathological and physiological processes, NO produced by Bacillus anthracis may also have a pivotal pathophysiological role in anthrax infection671709--
additional information?-Leishmania amazonensis-the enzyme might be involved in the infectivity and/or escaping mechanism of the parasite671631--
additional information?-Drosophila melanogaster-dNOS participates in essential developmental and behavioral aspects of the fruit fly685095--
additional information?-Drosophila melanogaster-Drosophila dNOS is a more efficient and active NO synthase than the mammalian NOS enzymes, which may allow it to function more broadly in cell signaling and immune functions in the fruit fly685094--
additional information?-Rattus norvegicus-eNOS uncoupling is known to be controlled by substrate/cofactor availability, and the uncoupled reactions play important roles under various physiological/pathological conditions, such as atherosclerosis and septic shock687615--
additional information?-Rattus norvegicus-increased iNOS expression due to ethanol intake is responsible for gender differences in the vascular effects elicited by chronic ethanol consumption, while ovarian hormones do not play a role, overview685867--
additional information?-Mus musculus-inducible nitric-oxide synthase-derived NO contributes to the pathophysiology of intestinal inflammation in the colon684317--
additional information?-Mus musculus-iNOS modulates endothelin-1-dependent release of prostacyclin and inhibition of platelet aggregation ex vivo in the mouse, overview688600--
additional information?-Mus musculus-nitric-oxide synthase 2 interacts with CD74 and inhibits its cleavage by caspase during dendritic cell development687727--
additional information?-Bacillus subtilis, Bacillus anthracis-the bacterial enzyme, bNOS, lacks an essential reductase domain, that supplies electrons during NO biosynthesis, and is thus limited with respect to a pool of available redox partners, but does produce NO in living cells and accomplish this task by hijacking available cellular redox partners that are not normally committed to NO production, bacterial reductase also supports NO synthesis by the oxygenase domain of mammalian NOS expressed in Escherichia coli, overview687703--
additional information?-Mus musculus-the enzyme exclusively performs the nitric oxide synthesis, an essential biological mediator, and of peroxynitrite, a well known cytotoxic agent involved innumerouspathophysiological processes, NOSs have the unique ability to both produce and activate peroxynitrite, overview687548--
additional information?-Rattus norvegicus-three unique structural elements are involved in the catalytic suppression of NOS: an autoinhibitory element in the FMN binding module, a CD2A loop in the connecting subdomain, and a C-terminal extension or tail, the C-terminal tail of nNOS is a regulatory element that suppresses nNOS activities in the absence of bound calmodulin, it may help stabilize the FMN-shielded conformation by holding the FMN module up against the FNR module as required for inter-flavin electron transfer, mechanism, overview685120--
additional information?-Mus musculusP70313eNOS is an important negative regulator of AMP-activated protein kinase phosphorylation and intracellular H2O2 generation in endothelial cells701000--

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
(6R)-5,6,7,8-tetrahydro-L-biopterinHomo sapiens-; ; 696110 2D-image
(6R)-tetrahydrobiopterinMus musculus, Rattus norvegicus--658119 2D-image
(6R)-tetrahydrobiopterinBacillus anthracis-enzyme-bound671709 2D-image
(6R)-tetrahydrobiopterinDrosophila melanogaster-required685094 2D-image
2',3'-Dialdehyde analogue of NADPHRattus norvegicus-activation, can substitute for NADPH at low concentrations, inhibitory at concentrations of 40times the apparent Km-value or after prolonged incubation440196 2D-image
2,6-dichlorophenolindophenolSus scrofa-activation440199 2D-image
5,6,7,8-tetrahydro-L-biopterinMus musculus-enhances initial rate of NO-formation440193 2D-image
5,6,7,8-tetrahydro-L-biopterinHomo sapiens-stimulates 4fold at 0.001 mM440194 2D-image
5,6,7,8-tetrahydro-L-biopterinMus musculus-activity is correlated directly to bound biopterin concentration; enzyme purified in absence of biopterin contains substoichiometric concentration, if purified in presence of biopterin it contains 1 mol biopterin per mol MW 130000 subunit440195 2D-image
5,6,7,8-tetrahydro-L-biopterinRattus norvegicus-i.e. (6R)-2-amino-4-hydroxy-6-(L-erythro-1,2-dihydroxypropyl)-5,6,7,8-tetrahydropteridine, 6R-isomer, requirement, biopteroflavoprotein, 1 mol tetrahydrobiopterin per mol enzyme dimer440196 2D-image
5,6,7,8-tetrahydro-L-biopterinMus musculus--440198, 440201, 440236, 440239, 659336, 701000, 701194 2D-image
5,6,7,8-tetrahydro-L-biopterinRattus norvegicus--440198, 440203, 440213, 440230, 440236, 659330, 696643, 698484, 698852, 699996, 700433 2D-image
5,6,7,8-tetrahydro-L-biopterinSus scrofa--440199, 440211 2D-image
5,6,7,8-tetrahydro-L-biopterinBos taurus--440203, 440240, 696280, 698884 2D-image
5,6,7,8-tetrahydro-L-biopterinHomo sapiens--440205, 440236, 697713, 698461 2D-image
5,6,7,8-tetrahydro-L-biopterinMus musculus-presumably tightly enzyme-bound440206 2D-image
5,6,7,8-tetrahydro-L-biopterinRattus norvegicus-required440207, 440208, 440220 2D-image
5,6,7,8-tetrahydro-L-biopterinBos taurus-stimulates 9fold440214 2D-image
5,6,7,8-tetrahydro-L-biopterinHomo sapiens-required440215 2D-image
5,6,7,8-tetrahydro-L-biopterinHomo sapiens-absolute requirement, recombinant from Pichia pastoris440216 2D-image
5,6,7,8-tetrahydro-L-biopterinMus musculus-required440221 2D-image
5,6,7,8-tetrahydro-L-biopterinMus musculus-required; required for the first partial reaction, formation of NG-hydroxy-L-arginine440222 2D-image
5,6,7,8-tetrahydro-L-biopterinMus musculus-0.19 mol bound per mol of dimer; required440223 2D-image
5,6,7,8-tetrahydro-L-biopterinFelis catus-required440224 2D-image
5,6,7,8-tetrahydro-L-biopterinBos taurus-not required for activity440227 2D-image
5,6,7,8-tetrahydro-L-biopterinBos taurus-stimulates440229, 440235 2D-image
5,6,7,8-tetrahydro-L-biopterinOvis aries--440231 2D-image
5,6,7,8-tetrahydro-L-biopterinMus musculus-only wild-type440234 2D-image
5,6,7,8-tetrahydro-L-biopterinMus musculus-0.04 mol per mol of subunit440238 2D-image
5,6,7,8-tetrahydro-L-biopterinDrosophila melanogaster--657741 2D-image
5,6,7,8-tetrahydro-L-biopterinNicotiana tabacum--658405 2D-image
5,6,7,8-tetrahydro-L-biopterinBacillus subtilis--659336 2D-image
5,6,7,8-tetrahydro-L-biopterinRattus norvegicus-0.003 mM; 0.003 mM697412 2D-image
CalmodulinRattus norvegicus-activation, potent stimulator of purified, not crude, enzyme preparation440191 2D-image
CalmodulinRattus norvegicus-NADPH-diaphorase activity of the enzyme is Ca2+/calmodulin independent440196 2D-image
CalmodulinSus scrofa-the enzyme bears a Ca2+/calmodulin dependent FAD and FMN containing reductase domain which transfers electrons from NADPH to a variety of acceptors440199 2D-image
CalmodulinMus musculus-murine macrophage enzyme is Ca2+/calmodulin independent440200, 440201 2D-image
CalmodulinRattus norvegicus--440202, 440203, 440230, 440236, 675257, 696643, 698484, 698852, 700433 2D-image
CalmodulinBos taurus--440203 2D-image
CalmodulinHomo sapiens--440205, 440215, 440236, 697713, 698461 2D-image
CalmodulinRattus norvegicus-rat neutrophil enzyme is calmodulin independent440208, 440213 2D-image
CalmodulinBos taurus-Ca2+/calmodulin stimulates cytochrome c reductase activity440214 2D-image
CalmodulinHomo sapiens-dependent on, endothelial enzyme440216 2D-image
CalmodulinHomo sapiens-Ca2+/calmodulin is required for superoxide formation in absence of tetrahydropterin440218 2D-image
CalmodulinMus musculus-required440221, 440223, 440225 2D-image
CalmodulinFelis catus-required440224 2D-image
CalmodulinBos taurus-required440227 2D-image
CalmodulinRattus norvegicus-required440228 2D-image
CalmodulinOvis aries-required440231 2D-image
CalmodulinMus musculus-15fold stimulation of cytochrome c reduction of wild-type and mutants C415A and C415H; Ca2+/calmodulin stimulates cytochrome c reductase activity440234 2D-image
CalmodulinBos taurus-dependent on, endothelial enzyme440235 2D-image
CalmodulinMus musculus--440236, 701000, 701194 2D-image
CalmodulinHomo sapiens-no stimulation with exogenous calmodulin, inducible isoform from liver440237 2D-image
CalmodulinBos taurus-enzyme-bound is required, supplemented stimulates440240 2D-image
CalmodulinBacillus anthracis-enzyme-bound, the binding sequence links the two enzyme domains671709 2D-image
CalmodulinDrosophila melanogaster-dependent on685094 2D-image
CalmodulinDrosophila melanogaster--685095 2D-image
CalmodulinRattus norvegicus-dependent on687697 2D-image
CalmodulinHomo sapiens-; ; 696110 2D-image
CalmodulinBos taurus-maximum calmodulin-dependent activity is measured at 1.5 mM CaCl2, phosphorylation within an autoinhibitory domain in endothelial nitric oxide synthase reduces the Ca2+ concentrations required for calmodulin to bind and activate the enzyme696280 2D-image
CalmodulinRattus norvegicus-30 nM; 30 nM697412 2D-image
CalmodulinBos taurus-in the absence of calmodulin, the wild type enzyme activity is less than 15% of the maximum calmodulin-dependent values698884 2D-image
cytochrome cSus scrofa-activation440199 2D-image
cytochrome cDrosophila melanogaster--685095 2D-image
cytochrome cRattus norvegicus--687615 2D-image
cytochrome cBos taurus--687734 2D-image
FADMus musculus, Rattus norvegicus-FAD containing flavoprotein440192 2D-image
FADMus musculus-no activation by the addition of exogenous FAD; tightly enzyme-bound440193 2D-image
FADMus musculus-FAD containing flavoprotein; tightly enzyme-bound440195 2D-image
FADRattus norvegicus-1 mol FAD per mol enzyme dimer440196 2D-image
FADSus scrofa-the enzyme bears Ca2+/calmodulin dependent FAD and FMN containing reductase domain which transfers electrons from NADPH to a variety of acceptors440199 2D-image
FADMus musculus-2.2 mol FAD per mol of enzyme dimer; non-covalently bound FAD440201 2D-image
FADRattus norvegicus-absolute requirement for FAD440202 2D-image
FADMus musculus-1 mol per mol of enzyme subunit; FAD containing flavoprotein; slight activation by exogeneous FAD440206 2D-image
FADRattus norvegicus--440207, 440230, 674558, 675257 2D-image
FADRattus norvegicus-required440208 2D-image
FADMus musculus--440209, 440222, 440225, 440238, 440239, 674558 2D-image
FADHomo sapiens-required440215, 673804 2D-image
FADHomo sapiens-0.56 mol per mol of recombinant enzyme440216 2D-image
FADMus musculus-required440221 2D-image
FADMus musculus-0.49 mol per mol of dimer440223 2D-image
FADFelis catus--440224 2D-image
FADBos taurus-required440227 2D-image
FADBos taurus-major source of superoxide production in absence of tetrahydrobiopterin440229 2D-image
FADMus musculus-wild-type and mutant C415H contain1 mol per mol of subunit440234 2D-image
FADHomo sapiens-no flavin440237 2D-image
FADBos taurus--440240, 687734 2D-image
FADNicotiana tabacum--658405 2D-image
FADLeishmania amazonensis--671631 2D-image
FADBacillus anthracis-enzyme-bound671709 2D-image
FADHomo sapiensP29474-674558 2D-image
FADDrosophila melanogaster--685095 2D-image
FADMus musculus-required for catalysis687291 2D-image
FADBos taurusQ9BDQ7binding site sequence, overview690183 2D-image
FlavodoxinBacillus subtilis-reduced YkuN and YkuP containing FMN, YkuN is more efficient in supporting bsNOS catalysis, Km for YkuN is 0.0016 mM, for YkuP 0.022 mM, overview687579-
flavodoxin IBos taurusQ9BDQ7binding site sequence, overview690183-
FMNMus musculus, Rattus norvegicus-FMN containing flavoprotein440192 2D-image
FMNMus musculus-no activation by the addition of exogenous FMN; tightly enzyme-bound440193 2D-image
FMNMus musculus-FMN containing flavoprotein; tightly enzyme-bound440195 2D-image
FMNRattus norvegicus-1 mol FMN per mol enzyme dimer440196 2D-image
FMNSus scrofa-the enzyme bears Ca2+/calmodulin dependent FAD and FMN containing reductase domain which transfers electrons from NADPH to a variety of acceptors440199 2D-image
FMNMus musculus-1.1 mol FMN per mol enzyme dimer440201 2D-image
FMNMus musculus-1 mol per mol of enzyme subunit; FMN containing flavoprotein440206 2D-image
FMNMus musculus--440209, 440222, 440225, 440238, 440239 2D-image
FMNHomo sapiens-required440215, 673804 2D-image
FMNHomo sapiens-0.79 mol per mol of recombinant enzyme440216 2D-image
FMNMus musculus-required440221 2D-image
FMNMus musculus-0.71 mol per mol of dimer; required440223 2D-image
FMNFelis catus--440224 2D-image
FMNBos taurus-required440227 2D-image
FMNMus musculus-wild-type and mutant C415H contain 0.8 and 0.9 mol per mol of subunit, respectively440234 2D-image
FMNHomo sapiens-no flavin440237 2D-image
FMNBos taurus--440240, 687734 2D-image
FMNLeishmania amazonensis--671631 2D-image
FMNBacillus anthracis-enzyme-bound671709 2D-image
FMNHomo sapiensP29474FMN/heme electron transfer, FMN is capable of serving as a one electron heme reductant674558 2D-image
FMNMus musculus-FMN/heme electron transfer, FMN is capable of serving as a one electron heme reductant674558 2D-image
FMNRattus norvegicusP29476FMN/heme electron transfer, FMN is capable of serving as a one electron heme reductant674558 2D-image
FMNRattus norvegicus--675257 2D-image
FMNDrosophila melanogaster-regulation of the FMN module conformational equilibrium, overview685095 2D-image
FMNRattus norvegicus-an inverse correlation exists between FMN shielding and the cytochrome c reductase activity685120 2D-image
FMNMus musculus-required for catalysis687291 2D-image
FMNH2Rattus norvegicus--687697 2D-image
hemeRattus norvegicus--658119, 659257, 687697 2D-image
hemeMus musculus--658119 2D-image
hemeDrosophila melanogaster--685094 2D-image
hemeRattus norvegicus-an inverse correlation exists between FMN shielding and the cytochrome c reductase activity685120 2D-image
hemeTriatoma infestans--686010 2D-image
hemeMus musculus-the heme is coordinated by a cysteine residue on the proximal side, and the substrates, Arg or N-hydroxy-L-arginine, bind above the heme iron atom in the distal pocket, while the cofactor, tetrahydrobiopterin, binds along the side of the heme687291 2D-image
hemeBacillus subtilis, Staphylococcus aureus-frequencies of electron transfer, overview687572 2D-image
hemeBacillus subtilis--687579 2D-image
heme bBacillus subtilis, Staphylococcus aureus-bound, quantitative determination684957 2D-image
NADP+Bos taurus-binding mechanism687734 2D-image
NADPHRattus norvegicus--440190, 440191, 440192, 440198, 440203, 440208, 440213, 440220, 440228, 440230, 440236, 658119, 659257, 659330, 671278, 671728, 672363, 672524, 674558, 675257, 677252, 686293, 687615, 687697, 696643, 698484, 698852, 699996, 699997, 700433 2D-image
NADPHMus musculus--440192, 440193, 440195, 440198, 440200, 440201, 440206, 440209, 440217, 440219, 440221, 440222, 440225, 440234, 440236, 440238, 440239, 658119, 659336, 672016, 672524, 673662, 674558, 684317, 686293, 687548, 687727, 688600, 701000, 701194 2D-image
NADPHHomo sapiens--440194, 440205, 440216, 440218, 440219, 440236, 671728, 672524, 674558, 684277, 697713, 698461, 699439 2D-image
NADPHRattus norvegicus-requirement, specific for, NADPH-diaphorase activity requires higher NADPH concentrations than nitric oxide formation440196 2D-image
NADPHRattus norvegicus-dependent on440197, 440202, 440207 2D-image
NADPHSus scrofa--440199, 440211 2D-image
NADPHBos taurus--440203, 440214, 440227, 440229, 440235, 696280, 698884 2D-image
NADPHBos taurus, Mus musculus-NADPH-dependent dioxygenase440204 2D-image
NADPHRattus norvegicus-crude preparation requires only NADPH as cofactor; dependent on440210 2D-image
NADPHHomo sapiens-dependent on440215 2D-image
NADPHMus musculus-at high concentration inhibits dimer reconstitution from subunits; dependent on440223 2D-image
NADPHFelis catus-dependent on440224 2D-image
NADPHOvis aries--440231 2D-image
NADPHDrosophila melanogaster--657741, 685094 2D-image
NADPHNicotiana tabacum--658405 2D-image
NADPHBacillus subtilis--659336, 684957, 687572, 687579 2D-image
NADPHAplysia californica--659646 2D-image
NADPHLeishmania amazonensis--671631 2D-image
NADPHBacillus anthracis--671709 2D-image
NADPHRana pipiens--672844 2D-image
NADPHHomo sapiens-required673804 2D-image
NADPHGanoderma applanatum--673971 2D-image
NADPHCricetulus griseus--675520 2D-image
NADPHStaphylococcus aureus--684957, 687572 2D-image
NADPHDrosophila melanogaster-dependent on685095 2D-image
NADPHRattus norvegicus-binding structure of NADP(H) to wild-type and truncation mutant enzyme lacking parts of the C-terminus, overview685120 2D-image
NADPHTriatoma infestans--686010 2D-image
NADPHMus musculus-required for catalysis687291 2D-image
NADPHBos taurus-binding mechanism687734 2D-image
NADPHBos taurusQ9BDQ7binding site sequence, overview690183 2D-image
NADPHRattus norvegicus-; 695853 2D-image
NADPHHomo sapiens-; ; 696110, 700432 2D-image
NADPHRattus norvegicus-1 mM; 1 mM697412 2D-image
NADPHHomo sapiens-; 699457, 700490 2D-image
Nitroblue tetrazoliumSus scrofa-activation440199 2D-image
tetrahydrobiopterinHomo sapiens, Rattus norvegicus-oxidation product of BH4 is a protonated BH3 radical, key role of BH4 in protonation of Fe(II)-O2-, overview671728 2D-image
tetrahydrobiopterinMus musculus--672016 2D-image
tetrahydrobiopterinHomo sapiens-required673804 2D-image
tetrahydrobiopterinRattus norvegicus--675257, 687697 2D-image
tetrahydrobiopterinMus musculus-the cofactor tetrahydrobiopterin binds along the side of the heme687291 2D-image
tetrahydrobiopterinMus musculus-binding analysis687548 2D-image

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
Ca2+Rattus norvegicus-requirement, brain, not liver or lung440190
Ca2+Rattus norvegicus--440191, 440203, 440210, 440236, 675257
Ca2+Homo sapiens--440194, 440205, 440236
Ca2+Sus scrofa-the enzyme bears Ca2+/calmodulin dependent cytochrome P-450 reductase activity which catalyzes cytochrome c reduction440199
Ca2+Mus musculus-slight activation440201
Ca2+Rattus norvegicus-cerebellum enzyme is Ca2+-dependent440202
Ca2+Bos taurus--440203
Ca2+Bos taurus, Mus musculus-constitutive endothelial and inducible membrane bound macrophage enzyme are strictly Ca2+-dependent; insoluble enzyme; macrophage enzyme440204
Ca2+Rattus norvegicus-required, calmodulin independent440208
Ca2+Sus scrofa-activation only in the presence of calmodulin440211
Ca2+Bos taurus-activation only in the presence of calmodulin440214
Ca2+Homo sapiens-absolutely dependent on440215
Ca2+Homo sapiens-Ca2+/calmodulin is required for citrulline/NO formation and for superoxide formation in absence of tetrahydropterin440218
Ca2+Felis catus-required, calmodulin independent440224
Ca2+Bos taurus-absolutely dependent on440227
Ca2+Rattus norvegicus-required440228, 440230
Ca2+Ovis aries-required440231
Ca2+Rattus norvegicus-cerebellum enzyme is Ca2+-dependent; retina enzyme is dependent on Ca2+440232
Ca2+Mus musculus--440234, 440236
Ca2+Bos taurus-required440235, 440240
Ca2+Homo sapiens-no stimulation with exogenous Ca2+, inducible isoform from liver440237
Ca2+Nicotiana tabacum-required658405
Ca2+Staphylococcus aureus-required658767
Ca2+Aplysia californica--659646
Ca2+Leishmania amazonensis--671631
Ca2+Homo sapiens-activates684277
Ca2+Drosophila melanogaster-dependent on685094
Ca2+Drosophila melanogaster-required685095
Ca2+Triatoma infestans--686010
Ca2+Mus musculus-required for catalysis687291
Ca2+Oreochromis mossambicus x Oreochromis niloticus-activates both isozymes687358
Ca2+Rattus norvegicus-required by eNOS, activates687615
Ca2+Rattus norvegicus-dependent on687697
Ca2+Bos taurus-required for calmodulin to bind and activate the enzyme, maximum calmodulin-dependent activity is measured at 1.5 mM CaCl2696280
Ca2+Rattus norvegicus-low levels of Ca2+ stimulate nNOS activity699996
Ca2+Rattus norvegicus-stimulation of mtNOS can be achieved by elevating mitochondrial Ca2+ (0.01-0.1 mM)699997
Ca2+Homo sapiens-the raise of intracellular calcium levels increases nNOS dephosphorylation and enzymatic activity700432
Ca2+Mammalia-dependent, NOS1 and NOS3701646
Ca2+/calmodulinHomo sapiens-activate the electron transfer673804
Ca2+/calmodulinHomo sapiensP29474calmodulin activates electron transfer from NADPH through three reductase domains to the oxygenase domain, controls constitutive isoforms through regulation of electrontransfer between NADPH and heme674558
Ca2+/calmodulinMus musculus-calmodulin activates electron transfer from NADPH through three reductase domains to the oxygenase domain, controls constitutive isoforms through regulation of electrontransfer between NADPH and heme674558
Ca2+/calmodulinRattus norvegicusP29476calmodulin activates electron transfer from NADPH through three reductase domains to the oxygenase domain, controls constitutive isoforms through regulation of electron transfer between NADPH and heme674558
Fe2+Homo sapiens, Rattus norvegicus--671728
Fe2+Bacillus subtilis, Staphylococcus aureus-a heme enzyme, spectral comparison of the FeIII-NO and FeII-NO complexes of the bacterial NOSs, FeIII-NO complexes lack change in Fe-N-O frequencies upon (6R) 5,6,7,8-tetrahydro-L-biopterin binding to bacterial NOSs, overview. In the FeIII-NO complexes, both L-arginine and NOHA induced the Fe-N-O bending mode at nearly the same frequency as a result of a steric interaction between the substrates and the heme-bound NO, while in FeII-NO complexes the the Fe-N-O bending mode is no observed684957
Fe2+Drosophila melanogaster-a heme enzyme685094
Fe2+Drosophila melanogaster-required685095
Fe2+Rattus norvegicus-in the heme cofactor685120
Fe2+Triatoma infestans--686010
Fe2+Mus musculus-in the heme cofactor, substrate-ligand interaction in the Fe2+-O2 complex, overview687291
Fe2+Bacillus subtilis, Staphylococcus aureus-a heme enzyme, frequencies of electron transfer, overview687572
Fe2+Bacillus subtilis-a heme enzyme687579
Fe2+Rattus norvegicus-a cytochrome P450 enzyme687615
Fe2+Rattus norvegicus-a heme enzyme687697
IronMus musculus, Rattus norvegicus-2 mol iron-protoporphyrin IX per mol enzyme dimer, the heme-iron is ferric, EPR-and light absorbance spectroscopy440192
IronMus musculus-mouse macrophage enzyme: cytochrome P-450 type hemoprotein; protoporphyrin IX heme440198
IronRattus norvegicus-protoporphyrin IX heme440198
IronHomo sapiens-0.8 mol per mol of subunit; heme-iron440216
IronMus musculus-naturally occuring neuronal mutant with a 105-amino acid deletion in the heme-binding domain as a result of in-frame mutation by specific alternative splicing, contains heme, but shows no L-arginine and NADPH-dependent citrulline-forming activity in presence of Ca2+-promoted calmodulin, the heme coordination geometry is highly abnormal440217
IronHomo sapiens--440218
IronRattus norvegicus--440220
IronMus musculus-heme-iron440221
IronMus musculus-protoporphyrin IX heme440222, 440225
IronMus musculus-0.9-1.2 mol heme per mol of dimer; heme-iron; protoporphyrin IX heme; required440223
IronHomo sapiens-0.88 mol per mol of recombinant enzyme monomer440233
IronMus musculus-heme ligand is bound via C415440234
IronMus musculus-0.83 mol per mol of subunit; protoporphyrin IX heme440238
IronBos taurus-heme-iron440240
IronMus musculus-low-spin heme iron, L-arginine aud tetrahydrobiopterin bind and stabilize heme iron in five-coordinate high-spin state658119
IronRattus norvegicus-low-spin heme iron, L-arginine and tetrahydrobiopterin bind and stabilize heme iron in five-coordinate high-spin state658119
IronRattus norvegicus-ferric heme659257
IronRattus norvegicus-heme659330
IronBacillus anthracis-as heme iron in protoporphyrin IX /heme, heme–substrate interactions and heme-transitions, overview671709
IronMus musculus-heme iron672016
IronHomo sapiensP29474heme iron, FMN/heme electron transfer674558
IronMus musculus-heme iron, FMN/heme electron transfer674558
IronRattus norvegicusP29476heme iron, FMN/heme electron transfer674558
Mg2+Oreochromis mossambicus x Oreochromis niloticus-activates both isozymes687358
O2Homo sapiens-oxygen tension influences the activity673804
ZincHomo sapiens-0.43 mol per mol of subunit440216
Zn2+Mus musculus-bound by Cys104, Cys109, and Cys194 of isozyme iNOS oxygenase domain674558

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
(4S)-N-(4-amino-5-[aminoethyl]aminopentyl)-N''-nitroguanidineHomo sapiens-; 699457 2D-image
1,5,6,7-tetrahydro-2H-azepin-2-iminesRattus norvegicus--672524 2D-image
1-phenylimidazoleBos taurus-reversible inhibition of endothelial enzyme, competitive versus L-arginine and tetrahydrobiopterin, no inhibition of cytochrome c reduction440235 2D-image
2',3'-Dialdehyde of NADPHRattus norvegicus-at concentrations of 40times the apparent Km-value or after prolonged incubation, independent of Ca2+/calmodulin, L-arginine or tetrahydrobiopterin, NADPH prevents inhibition, the NADPH-diaphorase activity of the enzyme is less sensitive than the nitric oxide synthase activity440196 2D-image
2-aminopyridine derivativesMus musculusP29477highly selective inhibitors672524-
3,4-dihydro-1-isoquinolinaminesMus musculusP29477-672524-
3-bromo-7-nitroindazoleHomo sapiens-nNOS-specific inhibitor, complete inhibition at 0.01 mM696110 2D-image
3-[cis-4'-[(6''-aminopyridin-2''-yl)methyl]pyrrolidin-3'-ylamino]propan-1-olHomo sapiens-; 699457 2D-image
4-(3-amino-propoxy)-1-benzopyran-2-one hydrochloric acid saltHomo sapiens-IC50: 0.0076 mM672524 2D-image
4-(3-amino-propoxy)-1-benzopyran-2-one hydrochloric acid saltMus musculusP29477IC50: 0.0119 mM672524 2D-image
4-(3-amino-propoxy)-1-benzopyran-2-one hydrochloric acid saltRattus norvegicus-IC50: 0.0091 mM672524 2D-image
4-(3-amino-propoxy)-6-chloro-1H-quinolin-2-one trifluoroacetic acid saltMus musculusP29477IC50: 410 nM, pharmacokinetic profile672524 2D-image
4-(3-dimethylamino-propoxy)-1-benzopyran-2-one hydrochloric acid saltHomo sapiens-IC50: 0.004 mM672524 2D-image
4-(3-dimethylamino-propoxy)-1-benzopyran-2-one hydrochloric acid saltMus musculusP29477IC50: 0.01 mM672524 2D-image
4-(3-dimethylamino-propoxy)-1-benzopyran-2-one hydrochloric acid saltRattus norvegicus-IC50: 0.01 mM672524 2D-image
4-(3-dimethylamino-propoxy)-1H-quinolin-2-oneHomo sapiens-IC50: 0.0026 mM672524 2D-image
4-(3-dimethylamino-propoxy)-1H-quinolin-2-oneMus musculusP29477IC50: 0.0104 mM672524 2D-image
4-(3-dimethylamino-propoxy)-1H-quinolin-2-oneRattus norvegicus-IC50: 0.010 mM672524 2D-image
5,6,7,8-tetrahydrobiopterinRattus norvegicus-quenches the uncoupled reactions and results in much less reactive oxygen species formation, whereas the presence of redox-incompetent 7,8-dihydrobiopterin demonstrates little quenching effect687615 2D-image
6(R,S)-methyl-5-deazatetrahydropterinMus musculus--440195 2D-image
6-chloro-4-(3-aminopropoxy)-1-benzopyran-2-one trifluoroacetic acid saltHomo sapiens-IC50: 90 nM, pharmacokinetic profile672524 2D-image
6-chloro-4-(3-aminopropoxy)-1-benzopyran-2-one trifluoroacetic acid saltMus musculusP29477IC50: 60 nM, pharmacokinetic profile672524 2D-image
6-chloro-4-(3-aminopropoxy)-1-benzopyran-2-one trifluoroacetic acid saltRattus norvegicus-IC50: 0.00056 mM, pharmacokinetic profile672524 2D-image
6-chloro-4-(3-dimethylamino-propoxy)-1-benzopyran-2-one hydrochloric acid saltHomo sapiens-IC50: 0.0041 mM672524 2D-image
6-chloro-4-(3-dimethylamino-propoxy)-1-benzopyran-2-one hydrochloric acid saltMus musculusP29477IC50: 0.0012 mM672524 2D-image
6-chloro-4-(3-dimethylamino-propoxy)-1-benzopyran-2-one hydrochloric acid saltRattus norvegicus-IC50: 0.008 mM672524 2D-image
6-chloro-4-(3-methylamino-propoxy)-1-benzopyran-2-one trifluoroacetic acid saltHomo sapiens-IC50: 0.00011mM672524 2D-image
6-chloro-4-(3-methylamino-propoxy)-1-benzopyran-2-one trifluoroacetic acid saltMus musculusP29477IC50: 0.00025mM672524 2D-image
6-chloro-4-(3-methylamino-propoxy)-1-benzopyran-2-one trifluoroacetic acid saltRattus norvegicus-IC50: 0.00053mM672524 2D-image
6-n-propyl-2-thyouracilRattus norvegicusP294750.1 mg 6-n-propyl-2-thyouracil decreases nNOS activity to 45% compared to control700433 2D-image
7-nitroindazoleOvis aries-weak inhibition440231 2D-image
7-nitroindazoleBos taurus-reversible inhibition of endothelial enzyme, competitive versus tetrahydrobiopterin, no inhibition of cytochrome c reduction440235 2D-image
7-nitroindazoleHomo sapiens--660662 2D-image
7-nitroindazoleRattus norvegicus--671278 2D-image
7-nitroindazoleRattus norvegicus-neuronal NOS inhibitor697765 2D-image
7-nitroindazoleRattus norvegicus-inhibits the neuronal NOS in vivo and reduces L-DOPA-induced dyskinesias in a rat model of parkinsonism. The rats show a lack of tolerance for the anti-dyskinetic effects711646 2D-image
A-23187Rattus norvegicus-high levels of A-23187 inhibit nNOS activity699996 2D-image
agmatineHomo sapiens-at lower concentration than the Ki value agmatine leads to time-, concentration-, NADPH- and calmodulin-dependent inhibition of the neuronal enzyme in presence of calmodulin; causes an increase in NADPH oxidase activity of the enzyme440233 2D-image
AminoguanidineNicotiana tabacum--658405 2D-image
AminoguanidineStaphylococcus aureus-0.01 mM, about 40% residual activity658767 2D-image
AR-C102222Mus musculusP294771,2-dihydro-4-quinazolinamine derivative672524 2D-image
AR-C102222Rattus norvegicus-1,2-dihydro-4-quinazolinamine derivative672524 2D-image
AR-C85016Mus musculusP294771,2-dihydro-4-quinazolinamine derivative672524 2D-image
AR-C85016Rattus norvegicus-1,2-dihydro-4-quinazolinamine derivative672524 2D-image
AR-R17477Homo sapiens, Mus musculus, Rattus norvegicus--660371 2D-image
Ca2+Rattus norvegicus-preincubation at 37°C leads to time-dependent inhibition of the enzyme440228 2D-image
Ca2+Rattus norvegicus-high levels of Ca2+ inhibit nNOS activity699996 2D-image
CalcineurinRattus norvegicus--440228-
CalmidazoliumRattus norvegicus-calmodulin antagonist440197 2D-image
CalmidazoliumHomo sapiens--440205 2D-image
CalmidazoliumRattus norvegicus-in absence of calmodulin440232 2D-image
CalmidazoliumMus musculus-calmodulin antagonist; complete inhibition440239 2D-image
carbon monoxideRattus norvegicus-carbon monoxide down-regulates iNOS activity by reducing its expression level or by inhibiting its activity by converting it to an inactive P420 form, the presence of dithiothreitol, L-Arg, or H4B partially inhibits the iNOSP450 to iNOSP420 conversion, whereas the presence of both L-Arg and 5,6,7,8-tetrahydro-L-biopterin completely prevents the transition698484 2D-image
COMus musculus--440192, 440198, 440222 2D-image
CORattus norvegicus--440192 2D-image
CORattus norvegicus-partially purified rat cerebellum enzyme440198 2D-image
CO/O2Mus musculus-80%:20%, mixture440198-
cyanideHomo sapiens-heme-blocker inhibits superoxide formation after pretreatment of the enzyme440218 2D-image
cyanideRattus norvegicus-pretreatment440220 2D-image
Di-2-thienyliodoniumMus musculus-competitive, irreversible, complete, time and temperature dependent inhibition440209 2D-image
dimethylarginineMammalia--701646-
diphenylene iodoniumMus musculus-competitive, irreversible, complete, time and temperature dependent inhibition440209 2D-image
diphenylene iodoniumBos taurus-inhibition of superoxide production of recombinant isoform III440229 2D-image
EDTARattus norvegicus-brain enzyme440190 2D-image
EDTAMus musculus-no inhibition440201 2D-image
EDTAFelis catus-brain enzyme440224 2D-image
EDTABos taurus-inhibits at concentrations above 0.01 mM440240 2D-image
EGTALeishmania amazonensis--671631 2D-image
ethylene glycol bis(beta-amino-ethylether)-N,N,N',N'-tetraacetic acidMus musculus-i.e. EGTA, complete inhibition of cytosolic enzyme, partial inhibition of particulate enzyme440201, 440204 2D-image
ethylene glycol bis(beta-amino-ethylether)-N,N,N',N'-tetraacetic acidBos taurus-i.e. EGTA, complete inhibition of cytosolic enzyme, partial inhibition of particulate enzyme440204 2D-image
ethylene glycol bis(beta-amino-ethylether)-N,N,N',N'-tetraacetic acidRattus norvegicus-i.e. EGTA, complete inhibition of cytosolic enzyme, partial inhibition of particulate enzyme440207 2D-image
ethylene glycol bis(beta-amino-ethylether)-N,N,N',N'-tetraacetic acidFelis catus--440224 2D-image
Gly-methyl-L-arginineOreochromis mossambicus x Oreochromis niloticus-inhibition of the isozymes in absence or presence of L-arginine687358 2D-image
H2O2Homo sapiens-alters heme group, decrease in activity440233 2D-image
imidazoleHomo sapiens-heme-blocker inhibits superoxide formation after pretreatment of the enzyme440218 2D-image
imidazoleBos taurus-inhibition of the endothelial enzyme, competitive versus L-arginine, no inhibition of cytochrome c reduction440235 2D-image
imidazoleBacillus anthracis-the enzyme forms a sixcoordinate low-spin complex with inhibitor imidazole, interaction analysis671709 2D-image
IodoniumdiphenylMus musculus-competitive, irreversible, complete, time and temperature dependent inhibition440209 2D-image
L-arginineMus musculus-inhibits peroxynitrite activation687548 2D-image
L-arginineRattus norvegicus-L-arginine strongly stimulates oxygen consumption of eNOS and inhibits that of nNOS687615 2D-image
L-arginine methyl esterMus musculusP70313-701000 2D-image
L-Asn-methyl-L-arginineOreochromis mossambicus x Oreochromis niloticus-inhibition of the isozymes in absence or presence of L-arginine687358 2D-image
L-canavanineRattus norvegicus-liver enzyme, slight inhibition of brain enzyme440190 2D-image
L-canavanineOvis aries-not inhibitory440231 2D-image
L-N-methylarginineHomo sapiens-NOS inhibitor, complete inhibition at 0.5 mM; NOS inhibitor, complete inhibition at 0.5 mM; NOS inhibitor, complete inhibition at 0.5 mM696110 2D-image
L-N6-(1-iminoethyl)lysine dihydrochlorideAplysia californica-5 mM, 78% inhibition659646 2D-image
L-NG-nitro-arginine-methylesterOryctolagus cuniculus--661659-
L-Nomega-nitroarginine-(4R)-amino-L-proline amideHomo sapiens-; 699457 2D-image
L-Nomega-nitroarginine-2,4-L-diaminobutyramideHomo sapiens-; 699457 2D-image
L-omega-monomethyl L-arginineHomo sapiensQ62600potent competitive eNOS inhibitor, complete inhibition at 10 mM697688 2D-image
L-thiocitrullineAplysia californica-5 mM, above 95% inhibition659646 2D-image
L-thiocitrullineMus musculusP29477no isozyme specificity672524 2D-image
N(G),N(G)-dimethyl-L-arginineHomo sapiens-asymmetric dimethyl arginine660685 2D-image
N(G)-nitroarginine methyl esterMus musculusP70313-701194 2D-image
N-(4-aminobutyl)-5-chloro-2-naphthalene sulfonamideRattus norvegicus--440191 2D-image
N-(6-Aminohexyl)-1-naphthalene sulfonamideRattus norvegicus--440191 2D-image
N-(6-aminohexyl)-5-chloro-1-naphthalene sulfonamideFelis catus-calmodulin antagonist above 0.01 mM; i.e. W-7440224 2D-image
N-iminoethyl-L-lysineMus musculusP29477no isozyme specificity672524 2D-image
N-iminoethyl-L-ornithineMus musculusP29477no isozyme specificity672524 2D-image
N-monomethyl-L-arginineStaphylococcus aureus-0.01 mM, about 55% residual activity658767 2D-image
N-nitro-L-arginine methyl esterEntamoeba histolytica-1 mM, about 50% residual activity658664 2D-image
N-nitro-L-arginine methyl esterStaphylococcus aureus-0.01 mM, about 40% residual activity658767 2D-image
N-nitro-L-arginine methyl esterAplysia californica-0.5 mM, 65% inhibition659646 2D-image
N-nitro-L-arginine methyl esterHomo sapiens-competitive NOS inhibitor697713 2D-image
N-[(1,3-benzodioxol-5-yl)methyl]-1-[2-(1H-imidazol-1-yl)pyrimidin-4-yl]-4-(methoxycarbonyl)-piperazine-2-acetamideMus musculusP29477inhibition of dimer formation in vivo and in vitro, efficiency is dependent on enzyme source440219-
N1-[cis-4'-[(6''-amino-4''-methylpyridin-2''-yl)methyl]pyrrolidin-3'-yl]-N2-(4'-chlorobenzyl)ethane-1,2-diamineHomo sapiens-; 699457 2D-image
N1-[cis-4'-[(6''-aminopyridin-2''-yl)methyl]pyrrolidin-3'-yl]ethane-1,2-diamineHomo sapiens-; 699457 2D-image
N1-[trans-4'-[(6''-amino-4''-methylpyridin-2''-yl)methyl]pyrrolidin-3'-yl]-N2-(3'-chlorobenzyl)ethane-1,2-diamineHomo sapiens-; 699457 2D-image
NG-methyl arginineBacillus anthracis-specific inhibition671709 2D-image
NG-methyl-L-arginineMus musculusP29477no isozyme specificity672524 2D-image
Ng-monomethy-L-arginineMammalia--701646 2D-image
NG-Nitro-L-arginineMus musculusP29477no isozyme specificity672524 2D-image
NG-Nitro-L-arginineRattus norvegicus-complete inhibition at 1 mM, non-selective NOS inhibitor; complete inhibition at 1 mM, non-selective NOS inhibitor697412 2D-image
Ngamma,Ngamma-dimethyl-L-arginineBos taurus--440227 2D-image
Ngamma,Ngamma-dimethyl-L-arginineNicotiana tabacum--658405 2D-image
Ngamma-amino-L-arginineRattus norvegicus--440197 2D-image
Ngamma-hydroxy-Ngamma-methyl-L-arginineMus musculus-preincubation at 37°C leads to irreversible inactivation, substrates protect440193 2D-image
Ngamma-iminoethyl-L-ornithineRattus norvegicus-competitive inhibitor440210 2D-image
Ngamma-monomethyl-L-arginineRattus norvegicus--440190, 440197, 440203 2D-image
Ngamma-monomethyl-L-arginineBos taurus--440203, 440204, 440227 2D-image
Ngamma-monomethyl-L-arginineMus musculus--440204 2D-image
Ngamma-monomethyl-L-arginineHomo sapiens--440205, 440215 2D-image
Ngamma-monomethyl-L-arginineRattus norvegicus-not D-isomer, strong, competitive440210 2D-image
Ngamma-monomethyl-L-arginineBos taurus-inhibits citrulline formation, not cytochrome c reduction440214 2D-image
Ngamma-monomethyl-L-arginineFelis catus--440224 2D-image
Ngamma-monomethyl-L-arginineRattus norvegicus-slightly440230 2D-image
Ngamma-monomethyl-L-arginineOvis aries-slightly440231 2D-image
Ngamma-monomethyl-L-arginineHomo sapiens-endothelial and neuronal isoforms: reversible inhibition; L-arginine protects against enzyme inactivation, thus inactivation occurs at or near active site440236 2D-image
Ngamma-monomethyl-L-arginineMus musculus-inducible isoform: after preincubation irreversible, time- and concentration-dependent inactivation, without preincubation reversible inhibition; L-arginine protects against enzyme inactivation, thus inactivation occurs at or near active site440236 2D-image
Ngamma-monomethyl-L-arginineRattus norvegicus-endothelial and neuronal isoforms: reversible inhibition; in presence of tetrahydrobiopterin 0.004 mM the neuronal isoform is inactivated; L-arginine protects against enzyme inactivation, thus inactivation occurs at or near active site440236 2D-image
Ngamma-nitro-L-arginineBos taurus--440203, 440204, 440227 2D-image
Ngamma-nitro-L-arginineRattus norvegicus--440203 2D-image
Ngamma-nitro-L-arginineMus musculus--440204 2D-image
Ngamma-nitro-L-arginineHomo sapiens--440205 2D-image
Ngamma-nitro-L-arginineRattus norvegicus-competitive inhibitor440210 2D-image
Ngamma-nitro-L-arginineBos taurus-inhibits citrulline formation, not cytochrome c reduction440214 2D-image
Ngamma-nitro-L-arginineFelis catus--440224 2D-image
Ngamma-nitro-L-arginineOvis aries--440231 2D-image
Ngamma-nitro-L-arginineHomo sapiens-irreversible inactivation of neuronal and endothelial isoform after preincubation, unaffected by tetrahydrobiopterin; L-arginine protects against enzyme inactivation, thus inactivation occurs at or near active site440236 2D-image
Ngamma-nitro-L-arginineMus musculus-L-arginine protects against enzyme inactivation, thus inactivation occurs at or near active site; reversible inhibitor of inducible isoform from macrophage440236 2D-image
Ngamma-nitro-L-arginineRattus norvegicus-irreversible inactivation of neuronal and endothelial isoform after preincubation, unaffected by tetrahydrobiopterin; L-arginine protects against enzyme inactivation, thus inactivation occurs at or near active site440236 2D-image
Ngamma-nitro-L-arginine methyl esterHomo sapiens-only L-isomer, inhibits NO and citrulline production from L-arginine as well as superoxide formation in absence of tetrahydropterin440218 2D-image
Ngamma-nitro-L-arginine methyl esterBos taurus--440227 2D-image
Ngamma-nitro-L-arginine methyl esterRattus norvegicus-very slightly, only L-isomer and in presence of tetrahydrobiopterin and NADPH440230 2D-image
Ngamma-nitro-L-arginine methyl esterOvis aries--440231 2D-image
Ngamma-nitro-L-arginine methyl esterBos taurus-complete inhibition440240 2D-image
Ngamma-nitro-L-arginine methyl esterLeishmania amazonensis--671631 2D-image
Ngamma-nitro-L-arginine methyl esterRattus norvegicus-nearly complete inhibition at 0.5 mM672363 2D-image
Nitroblue tetrazoliumRattus norvegicus-potent non-competitive inhibitor, partially reversible by tetrahydrobiopterin440196 2D-image
Nitroblue tetrazoliumSus scrofa--440199 2D-image
Nitroblue tetrazoliumBos taurus--440214 2D-image
NOOvis aries-feedback inhibition440231 2D-image
Nomega-nitro-L-arginine methyl esterRattus norvegicus-nonselective NOS inhibitor697765 2D-image
Nomega-nitro-L-arginine methyl esterRattus norvegicus--699996 2D-image
NXN-188Homo sapiens-a dual-action oral therapeutic being developed for the treatment of acute migraine. The pharmacological mechanism of action of NXN-188 involves inhibition of both the neuronal nitric oxide synthase enzyme isoform and affinity for serotonin receptors. Clinical studies and pharmacokinetics, detailed overview711779-
PINRattus norvegicus-human protein enzyme inhibitor, recombinantly expressed in Escherichia coli, the recombinant CREB-binding protein-bound inhibitor protein is purified by calmodulin affinity and inhibits the enzyme to a high extent at 0.001 mM672363-
S-ethylisothioureaAplysia californica-5 mM, 85% inhibition659646 2D-image
S-ethylisothioureaRattus norvegicus-inducible NOS inhibitor697765 2D-image
tetrahydrobiopterinMus musculus-inhibits peroxynitrite activation687548 2D-image
thiocoumarinHomo sapiens-IC50: 0.018 mM672524 2D-image
thiocoumarinMus musculusP29477weak inhibitor672524 2D-image
TrifluoperazineRattus norvegicus--440191, 440228 2D-image
TrifluoperazineMus musculus-no inhibitor of macrophage enzyme440195 2D-image
TrifluoperazineHomo sapiens-inhibition in the presence of Ca2+, reversible by calmodulin440205 2D-image
TrifluoperazineRattus norvegicus-no inhibitor of macrophage enzyme440207 2D-image
TrifluoperazineBos taurus-inhibits cytochrome c reductase activity440214 2D-image
TrifluoperazineRattus norvegicus-in absence of calmodulin440232 2D-image
W7 hydrochlorideAplysia californica-5 mM, 50% inhibition659646 2D-image
methylisothioureaStaphylococcus aureus-0.01 mM, about 80% residual activity658767 2D-image
additional informationRattus norvegicus-no inhibitor of NADPH-diaphorase activity: methotrexate440196-
additional informationMus musculus-the macrophage enzyme is not inhibited by calmodulin antagonists (N-4-aminobutyl-), (N-6-aminohexyl)-5-chloro-2-naphthalene sulfonamide440201-
additional informationRattus norvegicus-the macrophage enzyme is not inhibited by calmodulin antagonists (N-4-aminobutyl-), (N-6-aminohexyl)-5-chloro-2-naphthalene sulfonamide; the macrophage enzyme is not inhibited by calmodulin antagonists (N-6-aminohexyl)-1-naphthalene sulfonamide440207, 440208-
additional informationBos taurus-Ngamma,Ngamma'-dimethyl-L-arginine has no inhibitory effect440227-
additional informationMus musculus-not inhibitory: N-nitro-L-arginine methyl ester, or specific inhibitor of inducible NOS, 1400W658793-
additional informationAplysia californica-not inhibitory: N-nitro-D-arginine methyl ester at 0.5 mM659646-
additional informationHomo sapiens-no inhibition by 4-(3-amino-propoxy)-6-chloro-1H-quinolin-2-one trifluoroacetic acid salt672524-
additional informationMus musculusP29477inhibitory activity for coumarin derivatives, inhibitor screening, overview672524-
additional informationRattus norvegicus-no inhibition by 4-(3-amino-propoxy)-6-chloro-1H-quinolin-2-one trifluoroacetic acid salt and thiocoumarin672524-
additional informationRana pipiens-fibroblast growth factor-2 treatment up-regulates the enzyme in tectume, but down-regulates it in the optic nerve, overview672844-
additional informationRattus norvegicus-ethanol intake reduces eNOS and increases iNOS protein levels, while mRNA levels remain unaffected in female rat aorta685867-
additional informationMus musculus, Rattus norvegicus-inhibition of PSD-95/nNOS interaction by the nNOSalpha beta-finger antibody686293-
additional informationMus musculus-although H4B binding seems unable to affect iNOSoxy capacity to activate peroxynitrite decomposition, the binding of Arg and citrulline at the distal side of the heme pocket drastically reduces peroxynitrite activation687548-
additional informationRattus norvegicus-anthrax lethal factor potentially cleaves the regions (L191-Q192 and D264-N265) close to the NH2-terminus of neuronal nitric oxide synthase; inducible nitric oxide synthase is resistant to anthrax lethal factor-mediated cleavage695853-
additional informationHomo sapiens-interleukin-1 induces the degradation of isozyme iNOS, iNOS protein levels in osteoarthritic chondrocytes decreases to 45.4% of the control upon treatment with interleukin-1 for 15 min, and are further reduced to 41.5% when the treatment period is extended to 2 h; tissue necrosis factor-alpha induces nNOS disappearance, interleukin-1 induces the degradation of nNOS700490-
additional informationMus musculusP70313there is decreased eNOS activity in tight-skin 1-mouse skin tissue701194-

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
(6R,S)-Methyl-tetrahydropterinMus musculus-activation in the absence of biopterin, not as effective as tetrahydrobiopterin440195 2D-image
A-23187Rattus norvegicus-low levels of A-23187 stimulate nNOS activity699996 2D-image
Acetylsalicylic acidHomo sapiens-maximal activation at 0.004 mM684277 2D-image
Ca2+/calmodulinHomo sapiens-activate the electron transfer673804-
Ca2+/calmodulinHomo sapiensP29474calmodulin activates electron transfer from NADPH through three reductase domains to the oxygenase domain, controls constitutive isoforms through regulation of electrontransfer between NADPH and heme674558-
Ca2+/calmodulinMus musculus-calmodulin activates electron transfer from NADPH through three reductase domains to the oxygenase domain, controls constitutive isoforms through regulation of electrontransfer between NADPH and heme674558-
Ca2+/calmodulinRattus norvegicusP29476calmodulin activates electron transfer from NADPH through three reductase domains to the oxygenase domain, controls constitutive isoforms through regulation of electron transfer between NADPH and heme674558-
CalmodulinDrosophila melanogaster-substrate 2,6-dichlorophenolindophenol, about 10fold increase in activity in presence of calmodulin657741 2D-image
CalmodulinNicotiana tabacum-required658405 2D-image
CalmodulinStaphylococcus aureus-required658767 2D-image
CalmodulinAplysia californica--659646 2D-image
CalmodulinHomo sapiens--660022 2D-image
CalmodulinRattus norvegicus-activates the neuronal NOS by binding and inhibiting the suppression through the C-terminal tail of the enzyme, overview685120 2D-image
CalmodulinMus musculus-required for catalysis687291 2D-image
CalmodulinRattus norvegicus-activates O2 consumption of eNOS687615 2D-image
dithiothreitolRattus norvegicus-requirement440207 2D-image
dithiothreitolMus musculus--440234, 440236, 440238 2D-image
dithiothreitolBos taurus--440235, 440240 2D-image
dithiothreitolHomo sapiens, Rattus norvegicus--440236 2D-image
interferon gammaMus musculus-activates440201, 440238-
L-arginineRattus norvegicus-L-arginine strongly stimulates oxygen consumption of eNOS and inhibits that of nNOS, nonhydrolyzable L-arginine analogues are not stimulatory687615 2D-image
tetrahydrobiopterinHomo sapiens--660022 2D-image
tetrahydrobiopterinBacillus subtilis, Staphylococcus aureus--687572 2D-image
thyroxinRattus norvegicusP294750.001 mg thyroxin significantly increases nNOS activity and nNOS protein level to 153% compared to control700433-
lipopolysaccharideMus musculus-from Escherichia coli, activates440238 2D-image
additional informationHomo sapiens-neopterin derivatives are completely inactive and do not bind to the enzyme440194, 440215-
additional informationRana pipiens-fibroblast growth factor-2 teratment up-regulates the enzyme in tectume, but down-regultes it in the optic nerve, overview672844-
additional informationRattus norvegicusQ06518hepatic isozyme iNOS expression is induced in chronic liver cirrhosis early stages677252-
additional informationBos taurusQ9BDQ7iNOS is induced by pathogens and their components, e.g. induction in alveolar macropages by infection with the intracellular pathogen Mycobacterium bovis690183-
additional informationHomo sapiens-short-term (20-30 min) treatment of endothelial cells with the synthetic NO donor (Z)-1-[2-(2-aminoethyl)-N-(2-aminoethyl)amino](diazen-1-ium-1,2-diolate) increases the Ser1177 phosphorylation of the constitutively expressed endothelial NOS and the production of endogenous NO generated by eNOS from L-arginine, the phosphorylation of eNOS is Akt-dependent and completely reverted by the phosphatidylinositol-3 kinase inhibitor LY-294002697713-

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.0008-2',3'-dialdehyde NADPHRattus norvegicus--440196 2D-image
0.012-adriamycinHomo sapiens-FAD-FMN domain, absence of calmodulin657670 2D-image
0.021-adriamycinHomo sapiens-full-length enzyme, absence of calmodulin657670 2D-image
0.043-adriamycinHomo sapiens-full-length enzyme, presence of calmodulin657670 2D-image
0.05-adriamycinHomo sapiens-FAD-NADPH domain657670 2D-image
0.078-adriamycinHomo sapiens-FAD-FMN domain, presence of calmodulin657670 2D-image
4e-06-CalmodulinBos taurus--440214 2D-image
0.0080.0084dichlorophenolindophenolSus scrofa--440199 2D-image
0.0080.0084dichlorophenolindophenolRattus norvegicus-with L-arginine440210 2D-image
0.0019-L-arginineHomo sapiens-mutant nNOS D597N/M336V699457 2D-image
0.0020.0028L-arginineRattus norvegicus--440191, 440203 2D-image
0.0020.0028L-arginineRattus norvegicus-NADPH440196 2D-image
0.0020.0028L-arginineMus musculus--440201 2D-image
0.0020.0028L-arginineBos taurus--440203 2D-image
0.0021-L-arginineBos taurus--440240 2D-image
0.0022-L-arginineRattus norvegicus-22°C, wild type658119 2D-image
0.0023-L-arginineBos taurus--440203 2D-image
0.0023-L-arginineMus musculus-22°C, wild type658119 2D-image
0.0024-L-arginineRattus norvegicus-22°C, mutant W678F658119 2D-image
0.0025-L-arginineRattus norvegicus-22°C, mutant W678A658119 2D-image
0.0028-L-arginineMus musculus--440201 2D-image
0.0029-L-arginineMus musculus-isoform III440226 2D-image
0.00330.0043L-arginineRattus norvegicus--440196, 440203 2D-image
0.00330.0043L-arginineBos taurus-NADPH, bovine440203 2D-image
0.0039-L-arginineHomo sapiens-recombinant from Pichia pastoris440216 2D-image
0.0044-L-arginineHomo sapiens-wild-type660022 2D-image
0.0052-L-arginineHomo sapiens-mutant E298D660022 2D-image
0.00746-L-arginineNicotiana tabacum-pH 7.0, 37°C658405 2D-image
0.0084-L-arginineRattus norvegicus--440210 2D-image
0.0091-L-arginineOvis aries-detrusor440231 2D-image
0.0099-L-arginineOvis aries-urethra440231 2D-image
0.011-L-arginineFelis catus-with FAD and tetrahydropterin440224 2D-image
0.0134-L-arginineStaphylococcus aureus-pH 6.5, 47.5°C658767 2D-image
0.0140.018L-arginineRattus norvegicus--440197, 440202 2D-image
0.0140.018L-arginineMus musculus--440206 2D-image
0.0140.018L-arginineBos taurus--440227 2D-image
0.022-L-arginineRattus norvegicus--440208 2D-image
0.022-L-arginineHomo sapiens-in presence of tetrahydrobiopterin and L-arginine440237 2D-image
0.0275-L-arginineMus musculus-22°C, mutant W457A658119 2D-image
0.03230.035L-arginineRattus norvegicus-NADPH, diaphorase activity440196 2D-image
0.03230.035L-arginineSus scrofa-cytochrome c reduction440199 2D-image
0.03230.035L-arginineRattus norvegicus--440207 2D-image
0.041-L-arginineFelis catus-without tetrahydropterin and FAD440224 2D-image
0.097-L-arginineRattus norvegicus-with Ca2+, 0.01 mM440230 2D-image
0.11-L-arginineRattus norvegicus-without Ca2+440230 2D-image
4.8-L-arginineRattus norvegicus-pH 7.6, 10°C, wild type659257 2D-image
5.4-L-arginineRattus norvegicus-pH 7.6, 10°C, mutant DELTA296nNOS659257 2D-image
68.5-L-arginineRattus norvegicus-pH 7.6, 10°C, mutant DELTA349nNOS659257 2D-image
0.01-menadioneHomo sapiens-FAD-FMN domain, absence of calmodulin; FAD-FMN domain, presence of calmodulin657670 2D-image
0.011-menadioneHomo sapiens-full-length enzyme, presence of calmodulin657670 2D-image
0.014-menadioneHomo sapiens-full-length enzyme, absence of calmodulin657670 2D-image
0.041-menadioneHomo sapiens-FAD-NADPH domain657670 2D-image
0.0073-mitomycin CHomo sapiens-full-length enzyme, absence of calmodulin657670 2D-image
0.013-mitomycin CHomo sapiens-full-length enzyme, presence of calmodulin657670 2D-image
0.015-mitomycin CHomo sapiens-FAD-FMN domain, absence of calmodulin657670 2D-image
0.028-mitomycin CHomo sapiens-FAD-NADPH domain657670 2D-image
0.0003-NADPHMus musculus--440201 2D-image
0.000383-NADPHNicotiana tabacum-pH 7.0, 37°C658405 2D-image
0.0004-NADPHBos taurus--440203, 440214 2D-image
0.001-NADPHRattus norvegicus-mutant D1393E659330 2D-image
0.0013-NADPHRattus norvegicus-mutant D1393N659330 2D-image
0.0025-NADPHRattus norvegicus-wild-type659330 2D-image
0.0029-NADPHRattus norvegicus-mutant D1393V659330 2D-image
0.0032-NADPHDrosophila melanogaster-CaM-bound recombinant eNOS685095 2D-image
0.0041-NADPHDrosophila melanogaster-CaM-free recombinant eNOS685095 2D-image
0.019-Ngamma-hydroxy-L-arginineBos taurus--440240 2D-image
0.028-Ngamma-hydroxy-L-arginineMus musculus-nonlinear regression analysis440193 2D-image
0.0314-Ngamma-hydroxy-L-arginineMus musculus-Eadie-Hofstee graph440193 2D-image
0.036-Ngamma-hydroxy-L-arginineMus musculus-25°C, with tetrahydrobiopterin440222 2D-image
0.129-Ngamma-hydroxy-L-arginineMus musculus-25°C, tetrahydrobiopterin-free440222 2D-image
0.016-Nitroblue tetrazoliumSus scrofa--440199 2D-image
2e-05-tetrahydrobiopterinRattus norvegicus--440196 2D-image
0.0002-tetrahydrobiopterinRattus norvegicus-22°C, wild type658119 2D-image
0.0016-tetrahydrobiopterinMus musculus-22°C, wild type658119 2D-image
0.025-tetrahydrobiopterinRattus norvegicus-22°C, mutant W678A658119 2D-image
0.1-tetrahydrobiopterinMus musculus-22°C, mutant W457A658119 2D-image
0.114-tetrahydrobiopterinRattus norvegicus-22°C, mutant W678A658119 2D-image
0.055-mitomycin CHomo sapiens-FAD-FMN domain, presence of calmodulin657670 2D-image
additional information-additional informationHomo sapiens, Rattus norvegicus-rapid kinetic analysis, stopped-flow measurements under high pressure671728-
additional information-additional informationHomo sapiens-biochemical pathway model, kinetic modeling, isozymes NOS1 and NOS3673804-
additional information-additional informationBacillus subtilis, Staphylococcus aureus-kinetics of NO and substrate binding, recombinant enzyme684957-
additional information-additional informationDrosophila melanogaster-kinetics modeling and single-turnover reactions, kinetics of heme transitions during Arg oxidation, product stoichiometry analysis, detailed overview685094-
additional information-additional informationDrosophila melanogaster-kinetics of flavin reduction of the recombinant enzyme, overview685095-
additional information-additional informationRattus norvegicus-kinetics685120-
additional information-additional informationMus musculus-kinetic analysis of the interaction between peroxynitrite and the oxygenase domain of inducible NOS at three different pH values, overview687548-
additional information-additional informationBacillus subtilis, Staphylococcus aureus-kinetics and kinetic modelling, stopped-flow measurements687572-
additional information-additional informationBacillus subtilis-kinetics of heme-NO complex formation in the stopped-flow reaction687579-
additional information-additional informationRattus norvegicus-kinetics, rapid-freeze method, overview687697-
additional information-additional informationBos taurus-kinetics of cytcochrome c reduction and of reaction with FMN and cytochrome c, overview, eNOS and nNOS differ slightly in their NADP(H) interaction and flavin thermodynamics, and show distinct electron transfer activities, two-state conformational equilibrium of the FMN subdomain, overview687734-
additional information-additional informationMammalia-Km-value for arginine about 0.003-0.02 mM701646-

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
4.8-adriamycinHomo sapiens-FAD-FMN domain, absence of calmodulin657670 2D-image
4.9-adriamycinHomo sapiens-FAD-NADPH domain657670 2D-image
5.3-adriamycinHomo sapiens-full-length enzyme, absence of calmodulin657670 2D-image
94-adriamycinHomo sapiens-FAD-FMN domain, presence of calmodulin657670 2D-image
95-adriamycinHomo sapiens-full-length enzyme, presence of calmodulin657670 2D-image
1.08-L-arginineRattus norvegicus--440196 2D-image
6.6-menadioneHomo sapiens-full-length enzyme, absence of calmodulin657670 2D-image
8-menadioneHomo sapiens-FAD-NADPH domain657670 2D-image
9.5-menadioneHomo sapiens-FAD-FMN domain, absence of calmodulin657670 2D-image
88-menadioneHomo sapiens-FAD-FMN domain, presence of calmodulin657670 2D-image
96-menadioneHomo sapiens-full-length enzyme, presence of calmodulin657670 2D-image
0.62-mitomycin CHomo sapiens-full-length enzyme, absence of calmodulin657670 2D-image
0.8-mitomycin CHomo sapiens-FAD-NADPH domain657670 2D-image
2.2-mitomycin CHomo sapiens-FAD-FMN domain, absence of calmodulin657670 2D-image
15.8-mitomycin CHomo sapiens-full-length enzyme, presence of calmodulin657670 2D-image
26-mitomycin CHomo sapiens-FAD-FMN domain, presence of calmodulin657670 2D-image
0.28-NADPHRattus norvegicus-DELTAG810 mutant enzyme, in the presence of 10 units/ml catalase and superoxide dismutase, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C698852 2D-image
0.32-NADPHRattus norvegicus-DELTAG810 mutant enzyme, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C698852 2D-image
2.33-NADPHRattus norvegicus-wild type enzyme, in the presence of 10 units/ml catalase and superoxide dismutase, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C698852 2D-image
2.57-NADPHRattus norvegicus-wild type enzyme, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C698852 2D-image
0.033-nitric oxideRattus norvegicus-DELTAG810 mutant enzyme, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C698852 2D-image
0.055-nitric oxideRattus norvegicus-DELTAG810 mutant enzyme, in the presence of 10 units/ml catalase and superoxide dismutase, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C698852 2D-image
0.92-nitric oxideRattus norvegicus-wild type enzyme, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C698852 2D-image
1.23-nitric oxideRattus norvegicus-wild type enzyme, in the presence of 10 units/ml catalase and superoxide dismutase, in 25 mM potassium phosphate, pH 7.5, with 100 mM KCl, at 25°C698852 2D-image
0.65-Nitroblue tetrazoliumRattus norvegicus-NADPH-diaphorase activity440196 2D-image

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.00015-(4S)-N-(4-amino-5-[aminoethyl]aminopentyl)-N''-nitroguanidineHomo sapiens-wild type nNOS699457 2D-image
0.08-(4S)-N-(4-amino-5-[aminoethyl]aminopentyl)-N''-nitroguanidineHomo sapiens-wild type eNOS699457 2D-image
0.05-1-phenylimidazoleBos taurus--440235 2D-image
0.0094-3-[cis-4'-[(6''-aminopyridin-2''-yl)methyl]pyrrolidin-3'-ylamino]propan-1-olHomo sapiens-wild type nNOS699457 2D-image
0.0492-3-[cis-4'-[(6''-aminopyridin-2''-yl)methyl]pyrrolidin-3'-ylamino]propan-1-olHomo sapiens-mutant nNOS D597N/M336V699457 2D-image
0.3666-3-[cis-4'-[(6''-aminopyridin-2''-yl)methyl]pyrrolidin-3'-ylamino]propan-1-olHomo sapiens-wild type eNOS699457 2D-image
0.0008-7-nitroindazoleBos taurus--440235 2D-image
0.66-agmatineHomo sapiens-at lower concentration than the Ki value agmatine leads to time-, concentration-, NADPH- and calmodulin-dependent inhibition of the neuronal enzyme in presence of calmodulin440233 2D-image
0.05-imidazoleBos taurus--440235 2D-image
0.0001-L-Nomega-nitroarginine-(4R)-amino-L-proline amideHomo sapiens-wild type nNOS699457 2D-image
0.11-L-Nomega-nitroarginine-(4R)-amino-L-proline amideHomo sapiens-wild type eNOS699457 2D-image
0.0003-L-Nomega-nitroarginine-2,4-L-diaminobutyramideHomo sapiens-wild type nNOS699457 2D-image
0.107-L-Nomega-nitroarginine-2,4-L-diaminobutyramideHomo sapiens-wild type eNOS699457 2D-image
2.2e-06-N-[(1,3-benzodioxol-5-yl)methyl]-1-[2-(1H-imidazol-1-yl)pyrimidin-4-yl]-4-(methoxycarbonyl)-piperazine-2-acetamideMus musculusP29477-440219-
8.5e-05-N1-[cis-4'-[(6''-amino-4''-methylpyridin-2''-yl)methyl]pyrrolidin-3'-yl]-N2-(4'-chlorobenzyl)ethane-1,2-diamineHomo sapiens-wild type nNOS699457 2D-image
0.0012-N1-[cis-4'-[(6''-amino-4''-methylpyridin-2''-yl)methyl]pyrrolidin-3'-yl]-N2-(4'-chlorobenzyl)ethane-1,2-diamineHomo sapiens-mutant nNOS D597N/M336V699457 2D-image
0.0852-N1-[cis-4'-[(6''-amino-4''-methylpyridin-2''-yl)methyl]pyrrolidin-3'-yl]-N2-(4'-chlorobenzyl)ethane-1,2-diamineHomo sapiens-wild type eNOS699457 2D-image
0.000388-N1-[cis-4'-[(6''-aminopyridin-2''-yl)methyl]pyrrolidin-3'-yl]ethane-1,2-diamineHomo sapiens-wild type nNOS699457 2D-image
0.0367-N1-[cis-4'-[(6''-aminopyridin-2''-yl)methyl]pyrrolidin-3'-yl]ethane-1,2-diamineHomo sapiens-mutant nNOS D597N/M336V699457 2D-image
0.4167-N1-[cis-4'-[(6''-aminopyridin-2''-yl)methyl]pyrrolidin-3'-yl]ethane-1,2-diamineHomo sapiens-wild type eNOS699457 2D-image
0.00025-N1-[trans-4'-[(6''-amino-4''-methylpyridin-2''-yl)methyl]pyrrolidin-3'-yl]-N2-(3'-chlorobenzyl)ethane-1,2-diamineHomo sapiens-wild type nNOS699457 2D-image
0.0061-N1-[trans-4'-[(6''-amino-4''-methylpyridin-2''-yl)methyl]pyrrolidin-3'-yl]-N2-(3'-chlorobenzyl)ethane-1,2-diamineHomo sapiens-mutant nNOS D597N/M336V699457 2D-image
0.0034-Ngamma,Ngamma-dimethyl-L-arginineBos taurus--440227 2D-image
0.00667-Ngamma,Ngamma-dimethyl-L-arginineNicotiana tabacum-pH 7.0, 37°C658405 2D-image
0.0265-Ngamma-hydroxy-Ngamma-methyl-L-arginineMus musculus--440193 2D-image
0.0012-Ngamma-iminoethyl-L-ornithineRattus norvegicus--440210 2D-image
0.00031-Ngamma-monomethyl-L-arginineFelis catus--440224 2D-image
0.0007-Ngamma-monomethyl-L-arginineRattus norvegicus--440210 2D-image
0.0007-Ngamma-monomethyl-L-arginineHomo sapiens-endothelial isoform440236 2D-image
0.0007-Ngamma-monomethyl-L-arginineRattus norvegicus--440236 2D-image
0.0018-Ngamma-monomethyl-L-arginineBos taurus--440214 2D-image
0.002-Ngamma-monomethyl-L-arginineRattus norvegicus-neuronal isoform in presence of 0.004 mM tetrahydrobiopterin440236 2D-image
0.0025-Ngamma-monomethyl-L-arginineBos taurus--440203 2D-image
0.0026-Ngamma-monomethyl-L-arginineMus musculus-inducible isoform from macrophage after preincubation440236 2D-image
0.0039-Ngamma-monomethyl-L-arginineMus musculus-inducible isoform from macrophage without preincubation440236 2D-image
0.0054-Ngamma-monomethyl-L-arginineBos taurus--440227 2D-image
0.0065-Ngamma-monomethyl-L-arginineRattus norvegicus-neuronal isoform440236 2D-image
2e-05-Ngamma-nitro-L-arginineHomo sapiens-irreversible inactivation of endothelial isoform after preincubation440236 2D-image
4.1e-05-Ngamma-nitro-L-arginineFelis catus--440224 2D-image
9e-05-Ngamma-nitro-L-arginineRattus norvegicus-irreversible inactivation of neuronal isoform after preincubation440236 2D-image
0.0002-Ngamma-nitro-L-arginineBos taurus--440214 2D-image
0.0004-Ngamma-nitro-L-arginineRattus norvegicus--440210 2D-image
0.0015-Ngamma-nitro-L-arginineBos taurus--440203, 440227 2D-image
0.0081-Ngamma-nitro-L-arginineMus musculus-reversible inhibition of inducible macrophage isoform440236 2D-image
0.0155-Ngamma-nitro-L-arginine methyl esterBos taurus--440227 2D-image
0.007-Nitroblue tetrazoliumBos taurus--440214 2D-image
0.0952-N1-[trans-4'-[(6''-amino-4''-methylpyridin-2''-yl)methyl]pyrrolidin-3'-yl]-N2-(3'-chlorobenzyl)ethane-1,2-diamineHomo sapiens-wild type eNOS699457 2D-image
additional information-Ng,Ng-dimethylarginineMammalia-value about 0.001-0.0016701646-
additional information-Ng-monomethy-L-arginineMammalia-value about 0.001-0.0016701646 2D-image

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.0076-4-(3-amino-propoxy)-1-benzopyran-2-one hydrochloric acid saltHomo sapiens-IC50: 0.0076 mM672524 2D-image
0.0091-4-(3-amino-propoxy)-1-benzopyran-2-one hydrochloric acid saltRattus norvegicus-IC50: 0.0091 mM672524 2D-image
0.0119-4-(3-amino-propoxy)-1-benzopyran-2-one hydrochloric acid saltMus musculusP29477IC50: 0.0119 mM672524 2D-image
0.00041-4-(3-amino-propoxy)-6-chloro-1H-quinolin-2-one trifluoroacetic acid saltMus musculusP29477IC50: 410 nM, pharmacokinetic profile672524 2D-image
0.004-4-(3-dimethylamino-propoxy)-1-benzopyran-2-one hydrochloric acid saltHomo sapiens-IC50: 0.004 mM672524 2D-image
0.01-4-(3-dimethylamino-propoxy)-1-benzopyran-2-one hydrochloric acid saltMus musculusP29477IC50: 0.01 mM672524 2D-image
0.01-4-(3-dimethylamino-propoxy)-1-benzopyran-2-one hydrochloric acid saltRattus norvegicus-IC50: 0.01 mM672524 2D-image
0.0026-4-(3-dimethylamino-propoxy)-1H-quinolin-2-oneHomo sapiens-IC50: 0.0026 mM672524 2D-image
0.01-4-(3-dimethylamino-propoxy)-1H-quinolin-2-oneRattus norvegicus-IC50: 0.010 mM672524 2D-image
0.0104-4-(3-dimethylamino-propoxy)-1H-quinolin-2-oneMus musculusP29477IC50: 0.0104 mM672524 2D-image
6e-05-6-chloro-4-(3-aminopropoxy)-1-benzopyran-2-one trifluoroacetic acid saltMus musculusP29477IC50: 60 nM, pharmacokinetic profile672524 2D-image
9e-05-6-chloro-4-(3-aminopropoxy)-1-benzopyran-2-one trifluoroacetic acid saltHomo sapiens-IC50: 90 nM, pharmacokinetic profile672524 2D-image
0.00056-6-chloro-4-(3-aminopropoxy)-1-benzopyran-2-one trifluoroacetic acid saltRattus norvegicus-IC50: 0.00056 mM, pharmacokinetic profile672524 2D-image
0.0012-6-chloro-4-(3-dimethylamino-propoxy)-1-benzopyran-2-one hydrochloric acid saltMus musculusP29477IC50: 0.0012 mM672524 2D-image
0.0041-6-chloro-4-(3-dimethylamino-propoxy)-1-benzopyran-2-one hydrochloric acid saltHomo sapiens-IC50: 0.0041 mM672524 2D-image
0.008-6-chloro-4-(3-dimethylamino-propoxy)-1-benzopyran-2-one hydrochloric acid saltRattus norvegicus-IC50: 0.008 mM672524 2D-image
0.00011-6-chloro-4-(3-methylamino-propoxy)-1-benzopyran-2-one trifluoroacetic acid saltHomo sapiens-IC50: 0.00011mM672524 2D-image
0.00025-6-chloro-4-(3-methylamino-propoxy)-1-benzopyran-2-one trifluoroacetic acid saltMus musculusP29477IC50: 0.00025mM672524 2D-image
0.00053-6-chloro-4-(3-methylamino-propoxy)-1-benzopyran-2-one trifluoroacetic acid saltRattus norvegicus-IC50: 0.00053mM672524 2D-image
0.018-thiocoumarinHomo sapiens-IC50: 0.018 mM672524 2D-image

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
3.35e-08-Rattus norvegicus-crude extract440228
3.05e-07-Rattus norvegicus-crude extract440230
9e-06-Mus musculus-liver mitochondria440239
1.7e-05-Mus musculus-liver440239
3.3e-05-Mus musculus-brain mitochondria440239
4.3e-05-Rattus norvegicus-crude extract440210
7.3e-05-Mus musculus-purified enzyme, without tetrahydropterin440195
0.00035-Mus musculus-purified enzyme, with tetrahydropterin440195
0.00054-Mus musculus-brain440239
0.00074-Rattus norvegicus-crude extract440197
0.0075-Triatoma infestans-brain homogenates686010
0.0098-Rattus norvegicus-purified enzyme440202
0.01-Sus scrofa-reductase activity440199
0.031-Mus musculus-purified recombinant enzyme, NADPH/O2-supported reduction of Ngamma-hydroxy-L-arginine, tetrahydrobiopterin-free, 25°C440222
0.031-Bos taurus-purified pancreatic enzyme440227
0.0353-Staphylococcus aureus-pH 6.5, 47.5°C658767
0.074-Homo sapiens-purified enzyme440205
0.12-Rattus norvegicus-NADPH-diaphorase activity440196
0.12-Rattus norvegicus-purified enzyme440208
0.12-Mus musculus-purified recombinant enzyme, H2O2-supported reduction of Ngamma-hydroxy-L-arginine, tetrahydrobiopterin-free, 25°C440222
0.142-Homo sapiens-wild-type660022
0.143-Bos taurus-purified recombinant enzyme440240
0.159-Homo sapiens-mutant E298D660022
0.17-Rattus norvegicus--440196
0.17-Mus musculus-purified recombinant enzyme, NADPH/O2-supported reduction of Ngamma-hydroxy-L-arginine, with tetrahydrobiopterin, 25°C440222
0.181-Homo sapiens-partially purified enzyme440194
0.19-Homo sapiens-purified recombinant enzyme440216
0.340.35Bos taurus--440203
0.340.35Homo sapiens-purified enzyme, recombinant, determined as NADPH-oxidase activity440216
0.41-Bos taurus-substrate Ngamma-hydroxy-L-arginine, purified enzyme440240
0.73-Sus scrofa-purified enzyme440211
0.815-Mus musculus-purified recombinant enzyme, H2O2-supported reduction of Ngamma-hydroxy-L-arginine, with tetrahydrobiopterin, 25°C440222
0.940.96Rattus norvegicus--440191
0.940.96Rattus norvegicus-purified enzyme440207
1-Homo sapiens-purified enzyme440233
1.06-Mus musculus-purified enzyme440201
1.1-Mus musculus-purified enzyme440223, 440225
1.62-Mus musculus-purified enzyme440206
1.9-Rattus norvegicus-purified enzyme440213
38-Homo sapiens-purified enzyme, inducible isoform, cytochrome c reductase activity440237
983.7-Nicotiana tabacum-pH 7.0, 37°C658405
additional information-Rattus norvegicus-activity in lung and liver only after induction by endotoxin440190
additional information-Mus musculus, Rattus norvegicus--440198
additional information-Mus musculus--440221
additional information-Ovis aries-activity in urinary tract tissues440231
additional information-Homo sapiens-assay method440233
additional information-Mus musculus-wild-type and mutants440234
additional information-Rattus norvegicus-activity in hypertensive and healthy rats, overview671278
additional information-Mus musculus-iNOS activities in wild-type and chimeric mice, both with induced colitis, overview684317
additional information-Drosophila melanogaster-steady-state catalytic activities of dNOSr685095
additional information-Bacillus subtilis-enzyme activities with different flavodoxins, overview687579
additional information-Bacillus anthracis, Bacillus subtilis--687703
additional information-Bos taurus-low eNOS activity might involve an altered interaction with NADP(H)687734

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
78Rattus norvegicus--440207, 440208
7-Homo sapiens-assay at440194
7-Triatoma infestans-assay at686010
7.2-Rattus norvegicus-assay at440190, 440210
7.4-Rattus norvegicus-assay at440197
7.4-Homo sapiens-assay at440218
7.4-Bos taurus--440227
7.4-Homo sapiens, Mus musculus, Rattus norvegicus-assay at440236
7.4-Mus musculus-assay at440239
7.4-Bos taurus-assay at440240
7.4-Rattus norvegicus-assay at671278
7.4-Leishmania amazonensis-assay at671631
7.4-Rattus norvegicus-assay at672363
7.4-Ganoderma applanatum-assay at673971
7.4-Rattus norvegicus-assay at675257
7.4-Cricetulus griseus-assay at675520
7.4-Homo sapiens-assay at684277
7.4-Mus musculus-ligand interaction assay at687548
7.4-Rattus norvegicus-assay at687615
7.57.6Drosophila melanogaster-assay at685094
7.5-Mus musculus-assay at440206
7.5-Felis catus--440224
7.5-Rattus norvegicus-assay at687697
7.6-Rattus norvegicus-assay at440228
7.6-Homo sapiensP29474assay at674558
7.6-Mus musculus-assay at674558
7.6-Rattus norvegicusP29476assay at674558
7.6-Drosophila melanogaster-assay at685095
7.6-Rattus norvegicus-assay at685120
7.6-Mus musculus-assay at687291
7.6-Bacillus subtilis, Staphylococcus aureus-assay at687572
7.6-Bacillus subtilis-assay at687579
7.6-Bos taurus-assay at687734
7.8-Mus musculus-assay at440223, 440238
8-Homo sapiens-assay at440215
additional information-Rattus norvegicus-pI: 5.6440208

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
6.68Felis catus--440224

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
1025Bos taurus-assay at687734
10-Drosophila melanogaster-assay at685094
10-Drosophila melanogaster-anaerobic pre-steady-state cytochrome c reduction assay at685095
22-Rattus norvegicus-assay at room temperature685120
22-Mus musculus-assay at room temperature687291, 687548
23-Homo sapiens-about, assay at440218
24-Rattus norvegicus-assay at687615
25-Mus musculus-assay at440222
25-Bos taurus-assay at440240
25-Leishmania amazonensis-assay at671631
25-Drosophila melanogaster-assay at685095
25-Bacillus subtilis, Staphylococcus aureus-assay at687572
30-Rattus norvegicus-assay at671278
32-Mus musculus-assay at440239
37-Rattus norvegicus-assay at440190, 440191
37-Mus musculus, Rattus norvegicus-assay at440192
37-Mus musculus-assay at440193
37-Homo sapiens-assay at440194
37-Mus musculus-assay at440195
37-Rattus norvegicus-assay at440196, 440197
37-Mus musculus, Rattus norvegicus-assay at440198
37-Sus scrofa-assay at440199
37-Mus musculus-assay at440200, 440201
37-Rattus norvegicus-assay at440202
37-Bos taurus, Rattus norvegicus-assay at440203
37-Bos taurus, Mus musculus-assay at440204
37-Homo sapiens-assay at440205
37-Mus musculus-assay at440206
37-Rattus norvegicus-assay at440207, 440208
37-Mus musculus-assay at440209
37-Rattus norvegicus-assay at440210
37-Sus scrofa-assay at440211
37-Rattus norvegicus-assay at440213
37-Homo sapiens-assay at440215
37-Mus musculus-assay at440223
37-Felis catus-assay at440224
37-Bos taurus--440227
37-Rattus norvegicus-assay at440228
37-Ovis aries-assay at440231
37-Rattus norvegicus-assay at440232
37-Homo sapiens, Mus musculus, Rattus norvegicus-assay at440236
37-Homo sapiens-assay at440237
37-Mus musculus-assay at440238
37-Rattus norvegicus-assay at672363
37-Ganoderma applanatum-assay at673971
37-Homo sapiensP29474assay at674558
37-Mus musculus-assay at674558
37-Rattus norvegicusP29476assay at674558
37-Rattus norvegicus-assay at675257
37-Cricetulus griseus-assay at675520
37-Homo sapiens-assay at684277
37-Triatoma infestans-assay at686010
37-Bacillus subtilis-assay at687579

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
A-549 cellHomo sapiens-Pseudomonas aeruginosa stimulates expression of inducible nitric oxide synthase by A-549 cells660685Manually annotated by BRENDA team
A-549 cellHomo sapiens-; ; 695513Manually annotated by BRENDA team
adrenal glandBos taurus--440203, 440226Manually annotated by BRENDA team
adrenal glandRattus norvegicus--440203Manually annotated by BRENDA team
adrenal glandHomo sapiens, Rattus norvegicus, Sus scrofa-isoform I440226Manually annotated by BRENDA team
adrenal medullaRattus norvegicus--697765Manually annotated by BRENDA team
amastigoteLeishmania amazonensis-axenic, high enzyme expression level671631Manually annotated by BRENDA team
aortaRattus norvegicus-thoracic, muscle685867Manually annotated by BRENDA team
arterioleHomo sapiens-isozyme NOS3673804Manually annotated by BRENDA team
astrocyteMus musculus--693776Manually annotated by BRENDA team
astrocyteRattus norvegicus--699996Manually annotated by BRENDA team
basidiocarpGanoderma applanatum--673971Manually annotated by BRENDA team
bladderRattus norvegicus-; 698343Manually annotated by BRENDA team
bloodMus musculus-iNOS684317Manually annotated by BRENDA team
blood plasmaMus musculusP70313-701194Manually annotated by BRENDA team
blood plateletHomo sapiens--684277Manually annotated by BRENDA team
brainRattus norvegicus--440190, 440191, 440192, 440196, 440197, 440198, 440203, 440210, 440212, 440213, 440220, 440228, 440232, 440236, 671278, 674558, 686293, 697042Manually annotated by BRENDA team
brainMus musculus--440192, 440198, 440226, 440236, 440239, 672524, 686293Manually annotated by BRENDA team
brainHomo sapiens-Ca2+-dependent isoform440194Manually annotated by BRENDA team
brainSus scrofa--440199, 440211Manually annotated by BRENDA team
brainRattus norvegicus-Ca2+-dependent isoform440202Manually annotated by BRENDA team
brainBos taurus--440203, 440226Manually annotated by BRENDA team
brainHomo sapiens--440205, 440236, 711779Manually annotated by BRENDA team
brainFelis catus--440224Manually annotated by BRENDA team
brainHomo sapiens, Rattus norvegicus, Sus scrofa-isoform I440226Manually annotated by BRENDA team
brainHomo sapiens, Rattus norvegicus-neuronal enzyme671728Manually annotated by BRENDA team
brainRattus norvegicus-nNOS685120Manually annotated by BRENDA team
brainTriatoma infestans--686010Manually annotated by BRENDA team
brainRattus norvegicus-nNOS is the predominant isozyme in brain695853Manually annotated by BRENDA team
brainHomo sapiens-; ; 700432Manually annotated by BRENDA team
brainRattus norvegicus-from rats with unilateral depletion of dopamine in the substantia nigra compacta treated with L-DOPA at 30 mg/kg body weight for 34 days, nNOS expression is restricted to neurons711646Manually annotated by BRENDA team
brain stemBos taurus, Rattus norvegicus-cerebrum shows higher activity than cerebellum440203Manually annotated by BRENDA team
central nervous systemAplysia californica--659646Manually annotated by BRENDA team
central nervous systemTriatoma infestans--686010Manually annotated by BRENDA team
cerebellumHomo sapiens--440194, 440236Manually annotated by BRENDA team
cerebellumRattus norvegicus--440196, 440197, 440198, 440202, 440203, 440220, 440232, 440236, 686293Manually annotated by BRENDA team
cerebellumSus scrofa--440199, 440211Manually annotated by BRENDA team
cerebellumBos taurus--440203Manually annotated by BRENDA team
cerebellumMus musculus--686293Manually annotated by BRENDA team
cerebellumOreochromis mossambicus x Oreochromis niloticus-nNOS687358Manually annotated by BRENDA team
cerebral cortexFelis catus--440224Manually annotated by BRENDA team
cerebral cortexRattus norvegicus--686293, 700433Manually annotated by BRENDA team
cerebral cortexMus musculus--686293Manually annotated by BRENDA team
cerebrumRattus norvegicus--440203, 440228Manually annotated by BRENDA team
cerebrumBos taurus--440203Manually annotated by BRENDA team
cerebrumOreochromis mossambicus x Oreochromis niloticus-nNOS687358Manually annotated by BRENDA team
cervical carcinoma cellHomo sapiens-cell line ME-180, constitutive expression440215Manually annotated by BRENDA team
CHO cellCricetulus griseus--675520Manually annotated by BRENDA team
chondrocyteHomo sapiens--440226Manually annotated by BRENDA team
chondrocyteHomo sapiens-iNOS is more frequently expressed in osteoarthritic than in normal chondrocytes, iNOS expression and activity in osteoarthritic chondrocytes is sub-maximal; nNOS is more frequently expressed in normal than in osteoarthritic chondrocytes700490Manually annotated by BRENDA team
colonMus musculus-iNOS684317Manually annotated by BRENDA team
corpus striatumMus musculus, Rattus norvegicus--686293Manually annotated by BRENDA team
dendritic cellMus musculus-bone marrow-derived687727Manually annotated by BRENDA team
dermal microvascular endothelial cellHomo sapiens-; ; 696110Manually annotated by BRENDA team
detrusorOvis aries--440231Manually annotated by BRENDA team
deutocerebrumTriatoma infestans-mainly in the lateral soma rind, surrounding the sensory glomeruli, partially in association with the antennal mechanosensory and motor neuropil686010Manually annotated by BRENDA team
diencephalonOreochromis mossambicus x Oreochromis niloticus-nNOS687358Manually annotated by BRENDA team
DLD-1 cellHomo sapiens--440226Manually annotated by BRENDA team
endothelial cellHomo sapiens--697713Manually annotated by BRENDA team
endothelial cellMus musculus--701000, 701194Manually annotated by BRENDA team
endothelial cellMammalia-NOS3701646Manually annotated by BRENDA team
endotheliumBos taurus, Mus musculus-aorta, cell culture440204Manually annotated by BRENDA team
endotheliumHomo sapiens--440216, 440218, 660022, 674558Manually annotated by BRENDA team
endotheliumBos taurus-isoform III440226, 440229Manually annotated by BRENDA team
endotheliumHomo sapiens-isoform III; liver, lung, adrenal glandcolon, isoform II; lung, uterus, stomach440226Manually annotated by BRENDA team
endotheliumMus musculus-isoform III; liver, lung, adrenal glandcolon, isoform II440226Manually annotated by BRENDA team
endotheliumRattus norvegicus-isoform III; liver, lung, adrenal glandcolon, isoform II; lung, uterus, stomach440226Manually annotated by BRENDA team
endotheliumSus scrofa-isoform III; lung, uterus, stomach440226Manually annotated by BRENDA team
endotheliumBos taurus-constitutive, from pulmonary artery440235Manually annotated by BRENDA team
endotheliumHomo sapiens-umbilical vein cells440236Manually annotated by BRENDA team
endotheliumMus musculus, Rattus norvegicus--440236Manually annotated by BRENDA team
endotheliumBos taurus--440240, 687734Manually annotated by BRENDA team
endotheliumBos taurus-comparison of endothelial and neuronal isoform658116Manually annotated by BRENDA team
endotheliumHomo sapiens, Rattus norvegicus-endothelial enzyme671728Manually annotated by BRENDA team
endotheliumHomo sapiens-vascular, constitutive expression of the endothelial isozyme in endothelial cells673002Manually annotated by BRENDA team
endotheliumHomo sapiens-specific isozyme eNOS674451Manually annotated by BRENDA team
endotheliumOreochromis mossambicus x Oreochromis niloticus-eNOS, endothelial cells of blood vessels687358Manually annotated by BRENDA team
forebrainRattus norvegicus-synaptosomal fraction440210Manually annotated by BRENDA team
ganglionTriatoma infestans-subesophageal and prothoracic686010Manually annotated by BRENDA team
glioblastoma cellHomo sapiens-cell line A-172, american type440219Manually annotated by BRENDA team
hair follicleMus musculusP70313-701194Manually annotated by BRENDA team
heartRattus norvegicus--671278Manually annotated by BRENDA team
heartOreochromis mossambicus x Oreochromis niloticus-eNOS687358Manually annotated by BRENDA team
HEK-293T cellHomo sapiens--673680Manually annotated by BRENDA team
hepatocyteHomo sapiens, Mus musculus, Rattus norvegicus-isoform II440226Manually annotated by BRENDA team
hepatocyteRattus norvegicusQ06518-677252Manually annotated by BRENDA team
hippocampusFelis catus--440224Manually annotated by BRENDA team
hippocampusMus musculus, Rattus norvegicus--686293Manually annotated by BRENDA team
HUVEC cellHomo sapiens--674451, 697713Manually annotated by BRENDA team
hypothalamusFelis catus--440224Manually annotated by BRENDA team
hypothalamusRattus norvegicus--697042Manually annotated by BRENDA team
kidneyHomo sapiens, Rattus norvegicus, Sus scrofa-macula densa cells, isoform I440226Manually annotated by BRENDA team
kidneyRattus norvegicus-renal cortex440230Manually annotated by BRENDA team
kidneyRattus norvegicus--671278Manually annotated by BRENDA team
kidneyOreochromis mossambicus x Oreochromis niloticus-anterior, iNOS687358Manually annotated by BRENDA team
kidneyRattus norvegicus-; 697412Manually annotated by BRENDA team
laterodorsal tegmental nucleusMus musculus, Rattus norvegicus--686293Manually annotated by BRENDA team
leafNicotiana tabacum-leaf extract658405Manually annotated by BRENDA team
liverRattus norvegicus--440190, 440226, 671278, 699997Manually annotated by BRENDA team
liverMus musculus--440226, 440239, 658793Manually annotated by BRENDA team
liverHomo sapiens--440226, 699439Manually annotated by BRENDA team
liverHomo sapiens-inducible isoform440237Manually annotated by BRENDA team
liverRattus norvegicusQ06518temporal expression of hepatic iNOS in liver cirrhosis, induced in rats by chronic bile duct ligation, overview677252Manually annotated by BRENDA team
lungRattus norvegicus--440190Manually annotated by BRENDA team
lungHomo sapiens-; ; 695513Manually annotated by BRENDA team
lungMus musculusP70313-701194Manually annotated by BRENDA team
macrophageMus musculus, Rattus norvegicus-cytokine-activated440192Manually annotated by BRENDA team
macrophageMus musculus--440193, 440195, 440198, 440204, 440206, 440209, 440234, 674558Manually annotated by BRENDA team
macrophageRattus norvegicus--440198, 440207, 440208, 440236Manually annotated by BRENDA team
macrophageMus musculus-Ca2+-independent form; cytokine-activated440200Manually annotated by BRENDA team
macrophageMus musculus-Ca2+-independent form440201Manually annotated by BRENDA team
macrophageBos taurus--440204, 440226Manually annotated by BRENDA team
macrophageHomo sapiens--440219, 440236Manually annotated by BRENDA team
macrophageMus musculusP29477cytokine-activated; RAW 264.7 cells440219Manually annotated by BRENDA team
macrophageMus musculus-RAW 264.7 cells440221, 440222, 440223, 440225, 440236, 440238Manually annotated by BRENDA team
macrophageHomo sapiens, Mus musculus, Rattus norvegicus-in liver, lung, kidney, isoform II440226Manually annotated by BRENDA team
macrophageHomo sapiens, Rattus norvegicus-inducible enzyme671728Manually annotated by BRENDA team
macrophageOreochromis mossambicus x Oreochromis niloticus-iNOS687358Manually annotated by BRENDA team
macrophageMus musculus-bone marrow-derived687727Manually annotated by BRENDA team
macrophageBos taurusQ9BDQ7alveolar690183Manually annotated by BRENDA team
macrophageRattus norvegicus-iNOS is the predominant isozyme in macrophages695853Manually annotated by BRENDA team
macrophageMammalia-NOS2701646Manually annotated by BRENDA team
mast cellHomo sapiens-isozyme NOS1673804Manually annotated by BRENDA team
mesencephalonFelis catus--440224Manually annotated by BRENDA team
neostriatumFelis catus--440224Manually annotated by BRENDA team
nerve fiberHomo sapiens-isozyme NOS1673804Manually annotated by BRENDA team
neuronMus musculus-central nervous system, natural variant with 105-amino acid deletion in the heme-binding domain440217Manually annotated by BRENDA team
neuronMus musculus--440226, 658119Manually annotated by BRENDA team
neuronHomo sapiens, Rattus norvegicus, Sus scrofa-peripheral nitrergic nerves, isoform I440226Manually annotated by BRENDA team
neuronHomo sapiens--440233, 711779Manually annotated by BRENDA team
neuronBos taurus-comparison of endothelial and neuronal isoform658116Manually annotated by BRENDA team
neuronRattus norvegicus--658119, 672363, 674558, 687697, 711646Manually annotated by BRENDA team
neuronRana pipiens-amacrine672844Manually annotated by BRENDA team
neuronHomo sapiens-isozyme NOS1673804Manually annotated by BRENDA team
neuronCricetulus griseus--675520Manually annotated by BRENDA team
neuronMus musculus, Rattus norvegicus-neuronal NOS, nNOS686293Manually annotated by BRENDA team
neuronOreochromis mossambicus x Oreochromis niloticus-nNOS687358Manually annotated by BRENDA team
neuronBos taurus--687734Manually annotated by BRENDA team
neuronal cell lineMammalia-NOS1701646Manually annotated by BRENDA team
neutrophilRattus norvegicus-peritoneal polymorphonuclear; polymorphonuclear440208, 440213Manually annotated by BRENDA team
neutrophilRattus norvegicus-polymorphonuclear neutrophils, PMN675257Manually annotated by BRENDA team
olfactory bulbOreochromis mossambicus x Oreochromis niloticus-nNOS687358Manually annotated by BRENDA team
optic nerveRana pipiens--672844Manually annotated by BRENDA team
pancreasBos taurus--440226, 440227Manually annotated by BRENDA team
pancreasHomo sapiens, Rattus norvegicus, Sus scrofa-islet cells, isoform I440226Manually annotated by BRENDA team
peritoneumRattus norvegicus--440208, 440213Manually annotated by BRENDA team
pituitary glandBos taurus--440203, 440214Manually annotated by BRENDA team
pituitary glandRattus norvegicus--440203Manually annotated by BRENDA team
ponsFelis catus--440224Manually annotated by BRENDA team
RAW-264.7 cellRattus norvegicus-; 695853Manually annotated by BRENDA team
RAW-264.7 cellRattus norvegicus--696643Manually annotated by BRENDA team
renal inner medullaRattus norvegicus-; 697412Manually annotated by BRENDA team
retinaRattus norvegicus-constitutive, soluble form440232Manually annotated by BRENDA team
retinaRana pipiens-distribution, overview, in retinorecipient tectal layer, before and after axotomy and fibroblast growth factor-2 application672844Manually annotated by BRENDA team
retinal ganglion cellRana pipiens--672844Manually annotated by BRENDA team
skeletal muscleRattus norvegicus--685867Manually annotated by BRENDA team
skinMus musculusP70313n the skin, eNOS is present in the epidermal layer, hair follicles and also in the endothelial cells lining the blood vessels701194Manually annotated by BRENDA team
smooth muscleRattus norvegicus--440226Manually annotated by BRENDA team
smooth muscleHomo sapiens-isozyme NOS3673804Manually annotated by BRENDA team
spinal cordHomo sapiens, Rattus norvegicus, Sus scrofa-isoform I440226Manually annotated by BRENDA team
spinal cordOreochromis mossambicus x Oreochromis niloticus-nNOS, nerve cell bodies and neuronal fibers687358Manually annotated by BRENDA team
spleenHomo sapiens, Mus musculus, Rattus norvegicus-red pulp, eosinophils and neutrophils, isoform II440226Manually annotated by BRENDA team
spleenOreochromis mossambicus x Oreochromis niloticus-iNOS687358Manually annotated by BRENDA team
substantia nigraHomo sapiens-; ; 700432Manually annotated by BRENDA team
sympathetic ganglionHomo sapiens, Rattus norvegicus, Sus scrofa-isoform I440226Manually annotated by BRENDA team
T-lymphocyteHomo sapiens--700937Manually annotated by BRENDA team
tectum mesencephaliRana pipiens-distribution, overview, before and after axotomy and fibroblast growth factor-2 application672844Manually annotated by BRENDA team
testisRattus norvegicus-eNOS immunoreactivity is detected in germ cells, Sertoli cells, Leydig cells and vascular endothelial cells of the testis; iNOS positive cells are detected in seminiferous epithelial cells, especially in germ cells of the testis695396Manually annotated by BRENDA team
thalamusFelis catus--440224Manually annotated by BRENDA team
umbilical vein endotheliumHomo sapiens-i.e. HUVEC cells674451Manually annotated by BRENDA team
ureterOvis aries--440231Manually annotated by BRENDA team
urethraOvis aries--440231Manually annotated by BRENDA team
urinary bladder endothelial cellRattus norvegicus-; 698343Manually annotated by BRENDA team
urinary bladder smooth muscleRattus norvegicus-; 698343Manually annotated by BRENDA team
urothelial cell lineRattus norvegicus-; 698343Manually annotated by BRENDA team
microglial cell lineHomo sapiens-; ; 700432Manually annotated by BRENDA team
additional informationMus musculus-3 distinct isoforms: 1. a membrane-associated, constitutive enzyme from tha vascular endothelium, 2. a soluble, constitutive enzyme from neuronal cells, 3. an endotoxin- and cytokine-inducible enzyme exemplified by that from murine macrophages; activity of constitutive enzymes is regulated by binding of calmodulin and Ca2+, the inducible enzyme is regulated by binding of calmodulin, not by Ca2+440222Manually annotated by BRENDA team
additional informationRattus norvegicus-3 isoforms: 1. neuronal, soluble isoform I is constitutively expressed in brain and other tissues and Ca2+-regulated, 2. soluble isoform II is usually not constitutively expressed, but inducible in macrophages and other cells, 3. isoform III is membrane-bound and expressed in endothelial cells440226, 440232Manually annotated by BRENDA team
additional informationBos taurus, Homo sapiens, Mus musculus, Sus scrofa-3 isoforms: 1. neuronal, soluble isoform I is constitutively expressed in brain and other tissues and Ca2+-regulated, 2. soluble isoform II is usually not constitutively expressed, but inducible in macrophages and other cells, 3. isoform III is membrane-bound and expressed in endothelial cells440226Manually annotated by BRENDA team
additional informationRattus norvegicusQ06518expression analysis in isolated hepatocytes, healthy liver, and cirrhotic liver, overview677252Manually annotated by BRENDA team
additional informationTriatoma infestans-immunohistochemic analysis of enzyme distribution, overview686010Manually annotated by BRENDA team
additional informationMus musculus, Rattus norvegicus-nNOS splicing variants tissue distribution, immunohistochemic analysis, overview686293Manually annotated by BRENDA team
additional informationOreochromis mossambicus x Oreochromis niloticus-no activity in gill, intestine, and liver687358Manually annotated by BRENDA team

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
cytoplasmRattus norvegicus-isozyme nNOS5737675257Manually annotated by BRENDA team
cytoplasmHomo sapiens-; 5737700490Manually annotated by BRENDA team
cytoplasmMammalia--5737701646Manually annotated by BRENDA team
cytosolMus musculus--5829440195, 440198, 440204, 440236Manually annotated by BRENDA team
cytosolRattus norvegicus--5829440196, 440198, 440202, 440208, 440210, 440213, 440230, 440232, 440236Manually annotated by BRENDA team
cytosolBos taurus--5829440204Manually annotated by BRENDA team
cytosolHomo sapiens--5829440205, 440215, 440233, 440236Manually annotated by BRENDA team
cytosolOvis aries--5829440231Manually annotated by BRENDA team
cytosolRattus norvegicus-; 5829697412Manually annotated by BRENDA team
membraneRattus norvegicus--16020440202, 440230Manually annotated by BRENDA team
membraneBos taurus, Mus musculus-constitutive endothelial enzyme: predominantly membrane-bound, inducible macrophage enzyme: equally distributed between cytosol and membrane, small constitutive membrane-bound portion in murine macrophages16020440204Manually annotated by BRENDA team
membraneHomo sapiens--16020673804Manually annotated by BRENDA team
membraneCricetulus griseus--16020675520Manually annotated by BRENDA team
membraneRattus norvegicus-total NOS activity is enriched in the intracellular membrane fraction at normal salt diet; total NOS activity is enriched in the intracellular membrane fraction at normal salt diet16020697412Manually annotated by BRENDA team
mitochondrionMus musculus--5739440239Manually annotated by BRENDA team
mitochondrionMus musculus-distinct enzyme not identical to other known isoforms5739658793Manually annotated by BRENDA team
mitochondrionRattus norvegicus--5739671278, 699997Manually annotated by BRENDA team
mitochondrionMus musculus-isozyme NOS1 contains a mitochondrial targeting signal5739673662Manually annotated by BRENDA team
nucleusFelis catus--5634440224Manually annotated by BRENDA team
nucleusRattus norvegicus-isozyme iNOS5634675257Manually annotated by BRENDA team
nucleusMammalia--5634701646Manually annotated by BRENDA team
plasma membraneRattus norvegicus-; 5886697412Manually annotated by BRENDA team
plasma membraneMammalia--5886701646Manually annotated by BRENDA team
rough endoplasmic reticulumMammalia--5791701646Manually annotated by BRENDA team
mitochondrionMammalia--5739701646Manually annotated by BRENDA team
additional informationMus musculus-3 distinct isoforms: 1. a membrane-associated, constitutive enzyme from the vascular endothelium, 2. a soluble, constitutive enzyme from neuronal cells, 3. an endotoxin- and cytokine-inducible enzyme exemplified by that from murine macrophages; activity of constitutive enzymes is regulated by binding of calmodulin and Ca2+, the inducible enzyme is regulated by binding of calmodulin, not by Ca2+-440222Manually annotated by BRENDA team
additional informationRattus norvegicus-3 isoforms: 1. neuronal, soluble isoform I is constitutively expressed in brain and other tissues and Ca2+-regulated, 2. soluble isoform II is usually not constitutively expressed, but inducible in macrophages and other cells, 3. isoform III is membrane-bound and expressed in endothelial cells-440226, 440232Manually annotated by BRENDA team
additional informationBos taurus, Homo sapiens, Mus musculus, Sus scrofa-3 isoforms: 1. neuronal, soluble isoform I is constitutively expressed in brain and other tissues and Ca2+-regulated, 2. soluble isoform II is usually not constitutively expressed, but inducible in macrophages and other cells, 3. isoform III is membrane-bound and expressed in endothelial cells-440226Manually annotated by BRENDA team
additional informationMus musculus-subcellular distribution of NOS1-673662Manually annotated by BRENDA team
additional informationRattus norvegicus-subcellular distribution of NOS and caveolin-1, nNOS and iNOS are co-localized with caveolin-1, overview-675257Manually annotated by BRENDA team

PDBSCOPCATHORGANISM
1m7v, downloadSCOP (1m7v)CATH (1m7v)Bacillus subtilis (strain 168)
1m7z, downloadSCOP (1m7z)CATH (1m7z)Bacillus subtilis (strain 168)
2amo, downloadSCOP (2amo)CATH (2amo)Bacillus subtilis (strain 168)
2an0, downloadSCOP (2an0)CATH (2an0)Bacillus subtilis (strain 168)
2an2, downloadSCOP (2an2)CATH (2an2)Bacillus subtilis (strain 168)
2fc1, downloadSCOP (2fc1)CATH (2fc1)Bacillus subtilis (strain 168)
2fc2, downloadSCOP (2fc2)CATH (2fc2)Bacillus subtilis (strain 168)
1d0c, downloadSCOP (1d0c)CATH (1d0c)Bos taurus
1d0o, downloadSCOP (1d0o)CATH (1d0o)Bos taurus
1d1v, downloadSCOP (1d1v)CATH (1d1v)Bos taurus
1d1w, downloadSCOP (1d1w)CATH (1d1w)Bos taurus
1d1x, downloadSCOP (1d1x)CATH (1d1x)Bos taurus
1d1y, downloadSCOP (1d1y)CATH (1d1y)Bos taurus
1dm6, downloadSCOP (1dm6)CATH (1dm6)Bos taurus
1dm7, downloadSCOP (1dm7)CATH (1dm7)Bos taurus
1dm8, downloadSCOP (1dm8)CATH (1dm8)Bos taurus
1dmi, downloadSCOP (1dmi)CATH (1dmi)Bos taurus
1dmj, downloadSCOP (1dmj)CATH (1dmj)Bos taurus
1dmk, downloadSCOP (1dmk)CATH (1dmk)Bos taurus
1ed4, downloadSCOP (1ed4)CATH (1ed4)Bos taurus
1ed5, downloadSCOP (1ed5)CATH (1ed5)Bos taurus
1ed6, downloadSCOP (1ed6)CATH (1ed6)Bos taurus
1foi, downloadSCOP (1foi)CATH (1foi)Bos taurus
1foj, downloadSCOP (1foj)CATH (1foj)Bos taurus
1fol, downloadSCOP (1fol)CATH (1fol)Bos taurus
1foo, downloadSCOP (1foo)CATH (1foo)Bos taurus
1fop, downloadSCOP (1fop)CATH (1fop)Bos taurus
1i83, downloadSCOP (1i83)CATH (1i83)Bos taurus
1nse, downloadSCOP (1nse)CATH (1nse)Bos taurus
1p6l, downloadSCOP (1p6l)CATH (1p6l)Bos taurus
1p6m, downloadSCOP (1p6m)CATH (1p6m)Bos taurus
1p6n, downloadSCOP (1p6n)CATH (1p6n)Bos taurus
1q2o, downloadSCOP (1q2o)CATH (1q2o)Bos taurus
1rs8, downloadSCOP (1rs8)CATH (1rs8)Bos taurus
1rs9, downloadSCOP (1rs9)CATH (1rs9)Bos taurus
1zzs, downloadSCOP (1zzs)CATH (1zzs)Bos taurus
1zzt, downloadSCOP (1zzt)CATH (1zzt)Bos taurus
2g6o, downloadSCOP (2g6o)CATH (2g6o)Bos taurus
2hx2, downloadSCOP (2hx2)CATH (2hx2)Bos taurus
2nse, downloadSCOP (2nse)CATH (2nse)Bos taurus
3dqs, downloadSCOP (3dqs)CATH (3dqs)Bos taurus
3dqt, downloadSCOP (3dqt)CATH (3dqt)Bos taurus
3e7s, downloadSCOP (3e7s)CATH (3e7s)Bos taurus
3jww, downloadSCOP (3jww)CATH (3jww)Bos taurus
3jwx, downloadSCOP (3jwx)CATH (3jwx)Bos taurus
3jwy, downloadSCOP (3jwy)CATH (3jwy)Bos taurus
3jwz, downloadSCOP (3jwz)CATH (3jwz)Bos taurus
3n5p, downloadSCOP (3n5p)CATH (3n5p)Bos taurus
3n5q, downloadSCOP (3n5q)CATH (3n5q)Bos taurus
3n5r, downloadSCOP (3n5r)CATH (3n5r)Bos taurus
3n5s, downloadSCOP (3n5s)CATH (3n5s)Bos taurus
3n5t, downloadSCOP (3n5t)CATH (3n5t)Bos taurus
3nld, downloadSCOP (3nld)CATH (3nld)Bos taurus
3nle, downloadSCOP (3nle)CATH (3nle)Bos taurus
3nlf, downloadSCOP (3nlf)CATH (3nlf)Bos taurus
3nlg, downloadSCOP (3nlg)CATH (3nlg)Bos taurus
3nlh, downloadSCOP (3nlh)CATH (3nlh)Bos taurus
3nli, downloadSCOP (3nli)CATH (3nli)Bos taurus
3nlt, downloadSCOP (3nlt)CATH (3nlt)Bos taurus
3nlu, downloadSCOP (3nlu)CATH (3nlu)Bos taurus
3nse, downloadSCOP (3nse)CATH (3nse)Bos taurus
3pnh, downloadSCOP (3pnh)CATH (3pnh)Bos taurus
3rqo, downloadSCOP (3rqo)CATH (3rqo)Bos taurus
3rqp, downloadSCOP (3rqp)CATH (3rqp)Bos taurus
4nse, downloadSCOP (4nse)CATH (4nse)Bos taurus
5nse, downloadSCOP (5nse)CATH (5nse)Bos taurus
6nse, downloadSCOP (6nse)CATH (6nse)Bos taurus
7nse, downloadSCOP (7nse)CATH (7nse)Bos taurus
8nse, downloadSCOP (8nse)CATH (8nse)Bos taurus
9nse, downloadSCOP (9nse)CATH (9nse)Bos taurus
1m9j, downloadSCOP (1m9j)CATH (1m9j)Homo sapiens
1m9k, downloadSCOP (1m9k)CATH (1m9k)Homo sapiens
1m9m, downloadSCOP (1m9m)CATH (1m9m)Homo sapiens
1m9q, downloadSCOP (1m9q)CATH (1m9q)Homo sapiens
1m9r, downloadSCOP (1m9r)CATH (1m9r)Homo sapiens
1niw, downloadSCOP (1niw)CATH (1niw)Homo sapiens
1nsi, downloadSCOP (1nsi)CATH (1nsi)Homo sapiens
2ll6, downloadSCOP (2ll6)CATH (2ll6)Homo sapiens
2ll7, downloadSCOP (2ll7)CATH (2ll7)Homo sapiens
2nsi, downloadSCOP (2nsi)CATH (2nsi)Homo sapiens
3e7g, downloadSCOP (3e7g)CATH (3e7g)Homo sapiens
3eah, downloadSCOP (3eah)CATH (3eah)Homo sapiens
3ej8, downloadSCOP (3ej8)CATH (3ej8)Homo sapiens
3hr4, downloadSCOP (3hr4)CATH (3hr4)Homo sapiens
3nos, downloadSCOP (3nos)CATH (3nos)Homo sapiens
4nos, downloadSCOP (4nos)CATH (4nos)Homo sapiens
1dd7, downloadSCOP (1dd7)CATH (1dd7)Mus musculus
1df1, downloadSCOP (1df1)CATH (1df1)Mus musculus
1dwv, downloadSCOP (1dwv)CATH (1dwv)Mus musculus
1dww, downloadSCOP (1dww)CATH (1dww)Mus musculus
1dwx, downloadSCOP (1dwx)CATH (1dwx)Mus musculus
1jwj, downloadSCOP (1jwj)CATH (1jwj)Mus musculus
1jwk, downloadSCOP (1jwk)CATH (1jwk)Mus musculus
1m8d, downloadSCOP (1m8d)CATH (1m8d)Mus musculus
1m8e, downloadSCOP (1m8e)CATH (1m8e)Mus musculus
1m8h, downloadSCOP (1m8h)CATH (1m8h)Mus musculus
1m8i, downloadSCOP (1m8i)CATH (1m8i)Mus musculus
1m9t, downloadSCOP (1m9t)CATH (1m9t)Mus musculus
1n2n, downloadSCOP (1n2n)CATH (1n2n)Mus musculus
1noc, downloadSCOP (1noc)CATH (1noc)Mus musculus
1nod, downloadSCOP (1nod)CATH (1nod)Mus musculus
1nos, downloadSCOP (1nos)CATH (1nos)Mus musculus
1qom, downloadSCOP (1qom)CATH (1qom)Mus musculus
1qw4, downloadSCOP (1qw4)CATH (1qw4)Mus musculus
1qw5, downloadSCOP (1qw5)CATH (1qw5)Mus musculus
1r35, downloadSCOP (1r35)CATH (1r35)Mus musculus
1vaf, downloadSCOP (1vaf)CATH (1vaf)Mus musculus
2bhj, downloadSCOP (2bhj)CATH (2bhj)Mus musculus
2nod, downloadSCOP (2nod)CATH (2nod)Mus musculus
2nos, downloadSCOP (2nos)CATH (2nos)Mus musculus
2oro, downloadSCOP (2oro)CATH (2oro)Mus musculus
2orp, downloadSCOP (2orp)CATH (2orp)Mus musculus
2orq, downloadSCOP (2orq)CATH (2orq)Mus musculus
2orr, downloadSCOP (2orr)CATH (2orr)Mus musculus
2ors, downloadSCOP (2ors)CATH (2ors)Mus musculus
2ort, downloadSCOP (2ort)CATH (2ort)Mus musculus
2y37, downloadSCOP (2y37)CATH (2y37)Mus musculus
3dwj, downloadSCOP (3dwj)CATH (3dwj)Mus musculus
3e65, downloadSCOP (3e65)CATH (3e65)Mus musculus
3e67, downloadSCOP (3e67)CATH (3e67)Mus musculus
3e68, downloadSCOP (3e68)CATH (3e68)Mus musculus
3e6l, downloadSCOP (3e6l)CATH (3e6l)Mus musculus
3e6n, downloadSCOP (3e6n)CATH (3e6n)Mus musculus
3e6o, downloadSCOP (3e6o)CATH (3e6o)Mus musculus
3e6t, downloadSCOP (3e6t)CATH (3e6t)Mus musculus
3e7i, downloadSCOP (3e7i)CATH (3e7i)Mus musculus
3e7m, downloadSCOP (3e7m)CATH (3e7m)Mus musculus
3e7t, downloadSCOP (3e7t)CATH (3e7t)Mus musculus
3eai, downloadSCOP (3eai)CATH (3eai)Mus musculus
3ebd, downloadSCOP (3ebd)CATH (3ebd)Mus musculus
3ebf, downloadSCOP (3ebf)CATH (3ebf)Mus musculus
3gof, downloadSCOP (3gof)CATH (3gof)Mus musculus
3nod, downloadSCOP (3nod)CATH (3nod)Mus musculus
3nqs, downloadSCOP (3nqs)CATH (3nqs)Mus musculus
3nw2, downloadSCOP (3nw2)CATH (3nw2)Mus musculus
1f20, downloadSCOP (1f20)CATH (1f20)Rattus norvegicus
1k2r, downloadSCOP (1k2r)CATH (1k2r)Rattus norvegicus
1k2s, downloadSCOP (1k2s)CATH (1k2s)Rattus norvegicus
1k2t, downloadSCOP (1k2t)CATH (1k2t)Rattus norvegicus
1k2u, downloadSCOP (1k2u)CATH (1k2u)Rattus norvegicus
1lzx, downloadSCOP (1lzx)CATH (1lzx)Rattus norvegicus
1lzz, downloadSCOP (1lzz)CATH (1lzz)Rattus norvegicus
1m00, downloadSCOP (1m00)CATH (1m00)Rattus norvegicus
1mmv, downloadSCOP (1mmv)CATH (1mmv)Rattus norvegicus
1mmw, downloadSCOP (1mmw)CATH (1mmw)Rattus norvegicus
1om4, downloadSCOP (1om4)CATH (1om4)Rattus norvegicus
1om5, downloadSCOP (1om5)CATH (1om5)Rattus norvegicus
1p6h, downloadSCOP (1p6h)CATH (1p6h)Rattus norvegicus
1p6i, downloadSCOP (1p6i)CATH (1p6i)Rattus norvegicus
1p6j, downloadSCOP (1p6j)CATH (1p6j)Rattus norvegicus
1p6k, downloadSCOP (1p6k)CATH (1p6k)Rattus norvegicus
1qw6, downloadSCOP (1qw6)CATH (1qw6)Rattus norvegicus
1qwc, downloadSCOP (1qwc)CATH (1qwc)Rattus norvegicus
1rs6, downloadSCOP (1rs6)CATH (1rs6)Rattus norvegicus
1rs7, downloadSCOP (1rs7)CATH (1rs7)Rattus norvegicus
1tll, downloadSCOP (1tll)CATH (1tll)Rattus norvegicus
1vag, downloadSCOP (1vag)CATH (1vag)Rattus norvegicus
1zvi, downloadSCOP (1zvi)CATH (1zvi)Rattus norvegicus
1zvl, downloadSCOP (1zvl)CATH (1zvl)Rattus norvegicus
1zzq, downloadSCOP (1zzq)CATH (1zzq)Rattus norvegicus
1zzr, downloadSCOP (1zzr)CATH (1zzr)Rattus norvegicus
1zzu, downloadSCOP (1zzu)CATH (1zzu)Rattus norvegicus
2g6h, downloadSCOP (2g6h)CATH (2g6h)Rattus norvegicus
2g6i, downloadSCOP (2g6i)CATH (2g6i)Rattus norvegicus
2g6j, downloadSCOP (2g6j)CATH (2g6j)Rattus norvegicus
2g6k, downloadSCOP (2g6k)CATH (2g6k)Rattus norvegicus
2g6l, downloadSCOP (2g6l)CATH (2g6l)Rattus norvegicus
2g6m, downloadSCOP (2g6m)CATH (2g6m)Rattus norvegicus
2g6n, downloadSCOP (2g6n)CATH (2g6n)Rattus norvegicus
2hx3, downloadSCOP (2hx3)CATH (2hx3)Rattus norvegicus
2hx4, downloadSCOP (2hx4)CATH (2hx4)Rattus norvegicus
3b3m, downloadSCOP (3b3m)CATH (3b3m)Rattus norvegicus
3b3n, downloadSCOP (3b3n)CATH (3b3n)Rattus norvegicus
3b3p, downloadSCOP (3b3p)CATH (3b3p)Rattus norvegicus
3dqr, downloadSCOP (3dqr)CATH (3dqr)Rattus norvegicus
3fc5, downloadSCOP (3fc5)CATH (3fc5)Rattus norvegicus
3hsn, downloadSCOP (3hsn)CATH (3hsn)Rattus norvegicus
3hso, downloadSCOP (3hso)CATH (3hso)Rattus norvegicus
3hsp, downloadSCOP (3hsp)CATH (3hsp)Rattus norvegicus
3jt3, downloadSCOP (3jt3)CATH (3jt3)Rattus norvegicus
3jt4, downloadSCOP (3jt4)CATH (3jt4)Rattus norvegicus
3jt5, downloadSCOP (3jt5)CATH (3jt5)Rattus norvegicus
3jt6, downloadSCOP (3jt6)CATH (3jt6)Rattus norvegicus
3jt7, downloadSCOP (3jt7)CATH (3jt7)Rattus norvegicus
3jt8, downloadSCOP (3jt8)CATH (3jt8)Rattus norvegicus
3jt9, downloadSCOP (3jt9)CATH (3jt9)Rattus norvegicus
3jta, downloadSCOP (3jta)CATH (3jta)Rattus norvegicus
3jws, downloadSCOP (3jws)CATH (3jws)Rattus norvegicus
3jwt, downloadSCOP (3jwt)CATH (3jwt)Rattus norvegicus
3jwu, downloadSCOP (3jwu)CATH (3jwu)Rattus norvegicus
3jwv, downloadSCOP (3jwv)CATH (3jwv)Rattus norvegicus
3jx0, downloadSCOP (3jx0)CATH (3jx0)Rattus norvegicus
3jx1, downloadSCOP (3jx1)CATH (3jx1)Rattus norvegicus
3jx2, downloadSCOP (3jx2)CATH (3jx2)Rattus norvegicus
3jx3, downloadSCOP (3jx3)CATH (3jx3)Rattus norvegicus
3jx4, downloadSCOP (3jx4)CATH (3jx4)Rattus norvegicus
3jx5, downloadSCOP (3jx5)CATH (3jx5)Rattus norvegicus
3jx6, downloadSCOP (3jx6)CATH (3jx6)Rattus norvegicus
3n2r, downloadSCOP (3n2r)CATH (3n2r)Rattus norvegicus
3n5v, downloadSCOP (3n5v)CATH (3n5v)Rattus norvegicus
3n5w, downloadSCOP (3n5w)CATH (3n5w)Rattus norvegicus
3n5x, downloadSCOP (3n5x)CATH (3n5x)Rattus norvegicus
3n5y, downloadSCOP (3n5y)CATH (3n5y)Rattus norvegicus
3n5z, downloadSCOP (3n5z)CATH (3n5z)Rattus norvegicus
3n60, downloadSCOP (3n60)CATH (3n60)Rattus norvegicus
3n61, downloadSCOP (3n61)CATH (3n61)Rattus norvegicus
3n62, downloadSCOP (3n62)CATH (3n62)Rattus norvegicus
3n63, downloadSCOP (3n63)CATH (3n63)Rattus norvegicus
3n64, downloadSCOP (3n64)CATH (3n64)Rattus norvegicus
3n65, downloadSCOP (3n65)CATH (3n65)Rattus norvegicus
3n66, downloadSCOP (3n66)CATH (3n66)Rattus norvegicus
3n67, downloadSCOP (3n67)CATH (3n67)Rattus norvegicus
3n68, downloadSCOP (3n68)CATH (3n68)Rattus norvegicus
3n69, downloadSCOP (3n69)CATH (3n69)Rattus norvegicus
3n6a, downloadSCOP (3n6a)CATH (3n6a)Rattus norvegicus
3n6b, downloadSCOP (3n6b)CATH (3n6b)Rattus norvegicus
3n6c, downloadSCOP (3n6c)CATH (3n6c)Rattus norvegicus
3n6d, downloadSCOP (3n6d)CATH (3n6d)Rattus norvegicus
3n6e, downloadSCOP (3n6e)CATH (3n6e)Rattus norvegicus
3n6f, downloadSCOP (3n6f)CATH (3n6f)Rattus norvegicus
3n6g, downloadSCOP (3n6g)CATH (3n6g)Rattus norvegicus
3nlj, downloadSCOP (3nlj)CATH (3nlj)Rattus norvegicus
3nlk, downloadSCOP (3nlk)CATH (3nlk)Rattus norvegicus
3nlm, downloadSCOP (3nlm)CATH (3nlm)Rattus norvegicus
3nln, downloadSCOP (3nln)CATH (3nln)Rattus norvegicus
3nlo, downloadSCOP (3nlo)CATH (3nlo)Rattus norvegicus
3nlp, downloadSCOP (3nlp)CATH (3nlp)Rattus norvegicus
3nlq, downloadSCOP (3nlq)CATH (3nlq)Rattus norvegicus
3nlr, downloadSCOP (3nlr)CATH (3nlr)Rattus norvegicus
3nlv, downloadSCOP (3nlv)CATH (3nlv)Rattus norvegicus
3nlw, downloadSCOP (3nlw)CATH (3nlw)Rattus norvegicus
3nlx, downloadSCOP (3nlx)CATH (3nlx)Rattus norvegicus
3nly, downloadSCOP (3nly)CATH (3nly)Rattus norvegicus
3nlz, downloadSCOP (3nlz)CATH (3nlz)Rattus norvegicus
3nm0, downloadSCOP (3nm0)CATH (3nm0)Rattus norvegicus
3nny, downloadSCOP (3nny)CATH (3nny)Rattus norvegicus
3nnz, downloadSCOP (3nnz)CATH (3nnz)Rattus norvegicus
3pne, downloadSCOP (3pne)CATH (3pne)Rattus norvegicus
3pnf, downloadSCOP (3pnf)CATH (3pnf)Rattus norvegicus
3png, downloadSCOP (3png)CATH (3png)Rattus norvegicus
3q99, downloadSCOP (3q99)CATH (3q99)Rattus norvegicus
3q9a, downloadSCOP (3q9a)CATH (3q9a)Rattus norvegicus
3rqj, downloadSCOP (3rqj)CATH (3rqj)Rattus norvegicus
3rqk, downloadSCOP (3rqk)CATH (3rqk)Rattus norvegicus
3rql, downloadSCOP (3rql)CATH (3rql)Rattus norvegicus
3rqm, downloadSCOP (3rqm)CATH (3rqm)Rattus norvegicus
3rqn, downloadSCOP (3rqn)CATH (3rqn)Rattus norvegicus
3svp, downloadSCOP (3svp)CATH (3svp)Rattus norvegicus
3svq, downloadSCOP (3svq)CATH (3svq)Rattus norvegicus
3tyl, downloadSCOP (3tyl)CATH (3tyl)Rattus norvegicus
3tym, downloadSCOP (3tym)CATH (3tym)Rattus norvegicus
3tyn, downloadSCOP (3tyn)CATH (3tyn)Rattus norvegicus
3tyo, downloadSCOP (3tyo)CATH (3tyo)Rattus norvegicus
3ufo, downloadSCOP (3ufo)CATH (3ufo)Rattus norvegicus
3ufp, downloadSCOP (3ufp)CATH (3ufp)Rattus norvegicus
3ufq, downloadSCOP (3ufq)CATH (3ufq)Rattus norvegicus
3ufr, downloadSCOP (3ufr)CATH (3ufr)Rattus norvegicus
3ufs, downloadSCOP (3ufs)CATH (3ufs)Rattus norvegicus
3uft, downloadSCOP (3uft)CATH (3uft)Rattus norvegicus
3ufu, downloadSCOP (3ufu)CATH (3ufu)Rattus norvegicus
3ufv, downloadSCOP (3ufv)CATH (3ufv)Rattus norvegicus
3ufw, downloadSCOP (3ufw)CATH (3ufw)Rattus norvegicus
4eux, downloadSCOP (4eux)CATH (4eux)Rattus norvegicus
1mjt, downloadSCOP (1mjt)CATH (1mjt)Staphylococcus aureus

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
19000-Homo sapiens-gel filtration684277
55000-Mus musculus-oxygenase subunit domain, gel filtration440221
74000-Mus musculus-reductase subunit domain, gel filtration440225
112000-Mus musculus-oxygenase subunit domain, gel filtration440225
120000-Oreochromis mossambicus x Oreochromis niloticus-isozyme iNOS, gel filtration687358
135000-Staphylococcus aureus-gel filtration658767
150000-Rattus norvegicus-gel filtration440208
150000-Homo sapiens-SDS-PAGE; SDS-PAGE700490
178000-Oreochromis mossambicus x Oreochromis niloticus-isozyme nNOS, gel filtration687358
200000-Rattus norvegicus-gel filtration440191
200000-Sus scrofa-above, gel filtration440211
250000-Mus musculus-gel filtration440201
260000-Mus musculus-gel filtration440206
300000-Bos taurus, Rattus norvegicus-gel filtration440203
300000-Rattus norvegicus-gel filtration440207
320000-Bos taurus-gel filtration440227
additional information-Mus musculus-dissociates irreversibly into subunits in the absence of L-arginine, FAD and tetrahydrobiopterin440201

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
?Sus scrofa-x * 160000, SDS-PAGE440211
?Bos taurus-x * 152000, SDS-PAGE, Western Blot440214
?Homo sapiens-x * 135000, SDS-PAGE440218
?Bos taurus-130000-133000, isoforms II and III, amino acid sequence determination440226
?Homo sapiens-130000-133000, isoforms II and III, amino acid sequence determination; x * 160000, isoform I, amino acid sequence determination440226
?Mus musculus-130000-133000, isoforms II and III, amino acid sequence determination440226
?Rattus norvegicus-130000-133000, isoforms II and III, amino acid sequence determination; x * 160000, isoform I, amino acid sequence determination440226
?Sus scrofa--440226
?Drosophila melanogaster-x * 152000, SDS-PAGE, plus a proteolytic artefact of 121000657741
?Nicotiana tabacum-x * 120000 + x * 115000, SDS-PAGE658405
?Aplysia californica-x * 160000, SDS-PAGE659646
?Bacillus anthracis-x * 43000, recombinant His6-tagged enzyme, SDS-PAGE671709
?Triatoma infestans-x * 134000686010
?Mus musculus-x * 35000, recombinant GST-tagged truncated enzyme, SDS-PAGE686293
?Homo sapiens-x * 15000, SDS-PAGE; x * 15000, SDS-PAGE700490
dimerMus musculus-2 * 130000, SDS-PAGE440201, 440206, 440221, 440223, 440238
dimerBos taurus-2 * 150000, SDS-PAGE, gel filtration at high salt concentration440203
dimerRattus norvegicus--440203
dimerHomo sapiens-2 * 160000, SDS-PAGE440205
dimerRattus norvegicus-2 * 150000, SDS-PAGE440207
dimerHomo sapiens-2 * 131000-133000, recombinant, SDS-PAGE440216
dimerMus musculus-2 * 56000, oxygenase subunit domain, SDS-PAGE440225
dimerBos taurus-2 * 160000, SDS-PAGE440227
dimerBos taurus-2 * 130000-150000, SDS-PAGE440240
dimerMus musculus-subunit disassembly and unfolding of the protein following a pH jump from 7.5 to 2.8672016
dimerHomo sapiensP29474-674558
dimerMus musculus-2 * 69000, iNOS, SDS-PAGE674558
dimerRattus norvegicusP294762 * 75000, nNOS, SDS-PAGE674558
dimerOreochromis mossambicus x Oreochromis niloticus-2 * 89000, nNOS, SDS-PAGE, bands of 47 kDa and 29 kDa also occur687358
dimerMammalia--701646
heterodimerStaphylococcus aureus-1 * 67000 + 1 * 64000, SDS-PAGE658767
monomerRattus norvegicus-1 * 150000, SDS-PAGE440191, 440202, 440203, 440208
monomerHomo sapiens-1 * 160000, SDS-PAGE440194
monomerBos taurus-1 * 150000, SDS-PAGE440203
monomerMus musculus-1 * 74000, reductase domain, SDS-PAGE440225
monomerHomo sapiens-1 * 19000, SDS-PAGE684277
monomerOreochromis mossambicus x Oreochromis niloticus-1 * 120000, iNOS, SDS-PAGE687358
additional informationMus musculus, Rattus norvegicus-significant amino acid sequence homology to NADPH-cytochrome P-450-type hemoprotein440198
additional informationHomo sapiens-gel filtration: native protein is a dimer, especially in presence of tetrahydrobiopterin and L-arginine, but dissociates at low temperature to monomers during gel electrophoresis440216
additional informationMus musculus-dimer formation, each subunit consists of: 1 oxygenase domain containing heme, tetrahydrobiopterin, substrate binding site and 1 reductase domain containing FAD, FMN, calmodulin, NADPH binding site; dimeric structure is required for enzyme activity, interaction between subunits via oxygenase domains440221, 440225
additional informationMus musculus-D-arginine inhibits reconstitution of dimer from subunits; dissociation of dimer into subunits at pH 6.8; subunit composition of dimeric enzyme440223
additional informationMus musculus-dimer formation, each subunit consists of: 1 oxygenase domain containing heme, tetrahydrobiopterin, substrate binding site and 1 reductase domain containing FAD, FMN, calmodulin, NADPH binding site440238
additional informationBacillus anthracis-bimodal enzyme, comprising of an N-terminal oxygenase domain that binds protoporphyrin IX /heme, 6R-tetrahydrobiopterin, and L-arginine, and a C-terminal reductase domain that binds FMN, FAD, and NADPH, the two domains are linked together by a calmodulin binding sequence671709
additional informationRattus norvegicus-nNOS reductase structure homology modeling, overview685120
additional informationRattus norvegicus-the splicing variant nNOSaalpha contains a specific beta-finger motif involved in PDZ domain scaffolding686293
additional informationBos taurusQ9BDQ7iNOS domain structure, overview690183

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
flavoproteinMus musculus--658119
flavoproteinDrosophila melanogaster--657741
additional informationDrosophila melanogaster-hemoprotein657741
flavoproteinRattus norvegicus--658119, 659330
additional informationRattus norvegicus-hemoprotein659330

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
in complex with inhibitor ARHomo sapiens-660371
sitting drop vapor diffusion method; sitting drop vapor diffusion methodHomo sapiens-699457
in complex with inhibitor ARMus musculus-660371
orthorhombic crystals from NOS oxygenase domain lacking the N-terminal 114 residues, preparation in presence of imidazole, structure analysis via x-ray diffraction, also with bound inhibitor N-[(1,3-benzodioxol-5-yl)methyl]-1-[2-(1H-imidazol-1-yl)pyrimidin-4-yl]-4-(methoxycarbonyl)-piperazine-2-acetamideMus musculusP29477440219
x-ray crystal structure of the heme-binding domains of neuronal wild-type and mutant with deletion in the heme-binding domainMus musculus-440217
in complex with inhibitor ARRattus norvegicus-660371

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
7.4-Rattus norvegicus-purified, t1/2: 6 h, 4°C440207
7.4-Rattus norvegicus-purified, t1/2: 3 h, 4°C440208, 440213
additional information-Mus musculus-subunit disassembly and unfolding of the protein following a pH jump from 7.5 to 2.8, kinetic unfolding mechanism of the inducible enzymes' oxygenase domain, the initial step of the reaction is the disruption of the iNOSCOD dimer, to generate heme-bound monomeric species in various degrees of unfolding, which is accompanied by the loss of two tetrahydrobiopterin cofactors, subsequent heme loss generates monomeric apoproteins exhibiting various degrees of unfolding, modeling of the denaturation process, overview672016

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
37-Mus musculus-t1/2: about 6 h440201
37-Mus musculus-tetrahydrobiopterin-free enzyme is not stable, therefore reaction is performed at 25°C440222

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
tetrahydrobiopterin stabilizes dimeric formBos taurus-440229
very stable against trypsin, reductase domain is much more susceptible to urea than oxygenase domainDrosophila melanogaster-657741
inducible isoform is unstable during purification in absence of L-arginine and tetrahydrobiopterin toward loss of the heme group and formation of low-spin speciesHomo sapiens-440237
(6R)-tetrahydro-L-biopterin stabilizes during purification at 4°CMus musculus-440195
bovine serum albumin stabilizes during refoldingMus musculus-440238
freezing inactivates, 50% v/v glycerol protectsMus musculus-440206
glycerol stabilizesMus musculus-440195, 440206
Mg2+ does not stabilizeMus musculus-440206
mutants less stable than wild-type, precipitation during incubation at 15°C, partial stabilization with NaCl, stabilization by purification in presence of L-arginine and tetrahydrobiopterinMus musculus-658119
ammonium sulfate precipitation results in remarkable loss of tetrahydrobiopterinRattus norvegicus-440196
bovine serum albumin stabilizesRattus norvegicus-440191
dithiothreitol stabilizesRattus norvegicus-440207
glycerol stabilizesRattus norvegicus-440191
heat-, diethylether- and alkali-labile, slightly acid resistant cytosolic factor of MW above 100000 and of little charge at neutral pH stabilizesRattus norvegicus-440213
mutants less stable than wild-type, precipitation during incubation at 15°C, partial stabilization with NaCl, stabilization by purification in presence of L-arginie and tetrahydrobiopterinRattus norvegicus-658119
unstable during purificationRattus norvegicus-440208
glycerol stabilizesSus scrofa-440211

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-85°C, 50 mM Tris (pH 7.4) with 10% (v/v) glycerol, 100 mM NaCl, 0.2 mM L-arginine, 0.0002 mM 5,6,7,8-tetrahydro-L-biopterin, 0.02 mM dithiothreitol, and 1 mM 2',3'-AMP (mixed isomers), up to 1 month, no detectable loss of catalytic activityBos taurus-696280
-80°C, diluted preparation stable overnightMus musculus-440201
-80°C, stable overnight in 50% v/v glycerolMus musculus-440206
4°C, 6 h stableMus musculus-440201
4°C, t1/2: 2 hMus musculus-440206
0°C, crude, t1/2: 2 days, purified t1/2: 2 h, with 1 mg/ml bovine serum albumin and 20% v/v glycerol as stabilizing agents, t1/2: 7 daysRattus norvegicus-440191
4°C, crude, stable for at least 24 hRattus norvegicus-440207, 440208
4°C, in the presence of one or more cytosolic factors at least 24 h stableRattus norvegicus-440213
4°C, purified, t1/2: 3 h at pH 7.4Rattus norvegicus-440208, 440213
4°C, purified, t1/2: 6 h at pH 7.4Rattus norvegicus-440207
-70°C, with 20% v/v glycerol at least 4 weeks stableSus scrofa-440211
4°C, unstableSus scrofa-440211

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
partialAplysia californica-659646
recombinant His6-tagged soluble enzyme from Escherichia coli strain BL21 (DE3) by nickel affinity chromatographyBacillus anthracis-671709
recombinant enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatographyBacillus subtilis-687579
recombinant His6-tagged bNOS from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, anion exchange chromatography, and gel filtrationBacillus subtilis-684957
-Bos taurus-440214, 440227
affinity chromatographyBos taurus-440203
Ni-NTA Sepharose column chromatography and 2'-5' ADP Sepharose resin column chromatographyBos taurus-696280
recombinant endothelial isoform from human embryonic kidney cellsBos taurus-440240
recombinant endothelial isoform III from insect cellsBos taurus-440229
recombiant His-tagged dNOS from Escherichia coli by nickel affinity and anion exchange chromatography in the presence of L-Arg and H4BDrosophila melanogaster-685094
recombinant His-tagged construct dNOSr from Escherichia coli strain BL21 by nickel affinity and calmodulin affinity chromatographyDrosophila melanogaster-685095
affinity and size exclusion chromatographyHomo sapiens-440205
native enzyme from platelets activated by acetylsalicylic acid, by solubilization with Triton X-100, anion exchange chromatography, and gel filtration, to homogeneityHomo sapiens-684277
Ni Sepharose column chromatography, 2',5'-ADP Sepharose column chromatography, and Superdex 200 gel filtration; Ni Sepharose column chromatography, 2',5'-ADP Sepharose column chromatography, and Superdex 200 gel filtrationHomo sapiens-699457
partial, 2',5'-ADP-agarose affinity chromatographyHomo sapiens-440194
recombinant from Pichia pastorisHomo sapiens-440216
recombinant wild-type of inducible liver isoform from Escherichia coli with and without His-tag, requires inclusion of tetrahydrobiopterin in purification bufferHomo sapiens-440237
wild-type recombinant from insect cellsHomo sapiens-440218, 440233
native enzyme from axenic amastigotes by 2',5'-ADP affinity chromatography to homogeneityLeishmania amazonensis-671631
-Mus musculus-658793
2',5'-ADP-agarose affinity chromatographyMus musculus-440193, 440195, 440198
dimeric enzyme and subunitsMus musculus-440223, 440225
dimeric enzyme and subunits; from interferon-gamma- and lipopolysaccharide-activated macrophageMus musculus-440238
gel filtration chromatographyMus musculus-440201
heterodimerMus musculus-440221
inducible form from macrophage; sequential anion-exchange-, affinity chromatogaphyMus musculus-440206
partialMus musculus-440209
recombinant enzyme oxygenase domain from Escherichia coli in the absence of both Arg and tetrahydrobiopterinMus musculus-687291
recombinant from Escherichia coliMus musculus-440222
recombinant His-tagged or GST-tagged truncated nNOS, comprising residues 1-198, from Escherichia coli by nickel affinity or glutathione affinity chromatography, respectivelyMus musculus-686293
wild-type and mutants from insect cellsMus musculus-440234
wild-type and natural mutant, recombinant from Escherichia coli and insect cellsMus musculus-440217
native enzyme from tilapia, by 2',5'-ADP affinity chromatography, ion exchange chromatography, and gel filtrationOreochromis mossambicus x Oreochromis niloticus-687358
-Rattus norvegicus-440196, 440207
2',5'-ADP-affinity and anion exchange chromatographyRattus norvegicus-440191, 440208, 440213
2',5'-ADP-agarose affinity chromatographyRattus norvegicus-440192
2',5'-ADP-agarose affinity chromatography; partialRattus norvegicus-440198
; Rattus norvegicus-695853
cerebellum wild-type and recombinant brain enzymeRattus norvegicus-440220
Ni-NTA column chromatography, gel filtrationRattus norvegicus-698484
partially by mitochondria preparationRattus norvegicus-671278
recombinant eNOS from Escherichia coli by 2',5'-ADP affinity chromatographyRattus norvegicus-687615
recombinant His-tagged wild-type enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant truncation mutant from Escherichia coli strain BL21(DE3) by sequential 2',5'-ADP and calmodulin affinity chromatographyRattus norvegicus-685120
recombinant neuronal enzyme from HEK-293 cells by 2',5'-ADP affinity chromatography to about 90% purityRattus norvegicus-672363
recombinant nNOS from Escherichia coli in absence of tetrahydrobiopterinRattus norvegicus-687697
sequential affinity chromatography on 2',5'-ADP-agarose and calmodulin Sepharose 4BRattus norvegicus-440202
-Staphylococcus aureus-658767
recombinant bNOS by ammonium sulfate fractionation, anion exchange chromatography, and gel filtrationStaphylococcus aureus-684957
-Sus scrofa-440199
ammonium sulfate precipitation (30% saturation), 2',5'-ADP-affinity chromatographySus scrofa-440211

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
bacterial NOS, phylogenetic tree, functional expression in Escherichia coliBacillus anthracis-687703
DNA and amino acid sequence determination and analysis, functional expression of the C-terminally His6-tagged soluble enzyme in Escherichia coli strain BL21 (DE3), sequence alignment and comparative modelingBacillus anthracis-671709
bacterial NOS, phylogenetic tree, functional expression in Escherichia coliBacillus subtilis-687703
expression in Escherichia coli strain BL21(DE3)Bacillus subtilis-687579
overexpression of His6-tagged bNOS in Escherichia coli strain BL21(DE3)Bacillus subtilis-684957
expressed in Escherichia coli BL21(DE3) cellsBos taurus-696280
expression of endothelial isoform III in Spodoptera frugiperda cells via baculovirus infectionBos taurus-440229
expression of endothelial isoform in human embryonic kidney cellsBos taurus-440240
iNOS, DNA and amino acid sequence determination and analysis, phylogenetic analysisBos taurusQ9BDQ7690183
stable, functional co-expression of enzyme and NMDA receptors in CHO1 cell plasma membranes, predominantly, and in the cytosol of CHO1(–) cells lacking postsynaptic density 95 proteins, PSD-95, expressionCricetulus griseus-675520
expression of His-tagged dNOS in Escherichia coliDrosophila melanogaster-685094
phylogenetic tree, expression of a His-tagged construct, dNOSr, comprising the dNOS reductase domain and its adjacent calmodulin binding site in Escherichia coli strain BL21Drosophila melanogaster-685095
cell-specific gene regulation mechanism of the endothelial isozyme in the vascular endothelium involving endothelial-specific promoter, binding sites for AP-1, high affinity Sp1-binding sites and GATA promoter sites, and several, e.g. octameric, transcriptional regulators, epigenetic regulatory mechanisms in vascular endothelial cell-specific gene expression, overview, the cell-specific chromatin structure regulates transcription, and transcription factors, e.g. Sp1, AP-1, GATA-2, and octamer KLF, regulate transcription via chromatin-remodeling activity, overlapping cis antisense gene, endothelial-specific genetic regulation model, overviewHomo sapiens-673002
co-expression of the enzyme and Vac14 as GST- or His-tagged proteins in Escherichia colis train BL21, expression of Vac14 as FLAG-tagged protein in HEK-293T cellsHomo sapiens-673680
endothelial enzyme expressed in Pichia pastoris using a highly inducible alcohol oxidase promoter PAOX1Homo sapiens-440216
expressed in Escherichia coli BL21(DE3) cells; expressed in Escherichia coli BL21(DE3) cellsHomo sapiens-699457
expressed in SH-SY5Y cells; expressed in SH-SY5Y cells; expressed in SH-SY5Y cellsHomo sapiens-700432
expression in HUVE cells, vascular smooth muscle cells, and in HeLa cells, DNA methylation of proximal promoter sequences of eNOS promoter-beta-Gal fusion contructs in trangenic mice is not essential for cell-specific expression, endothelial cells are highly enriched in acetylated H3 and acetylated H4 at the eNOS promoter altering the promoter function and expressionHomo sapiens-674451
inducible liver isoform is expressed in Escherichia coli with and without His-tag, requires coexpression of calmodulinHomo sapiens-440237
neuronal enzyme expressed in Spodoptera frugiperda cells via baculovirus infectionHomo sapiens-440233
wild-type endothelial enzyme is expressed in insect cells via baculovirus infectionHomo sapiens-440218
expression in Escherichia coli, coexpression of calmodulin is necessaryMus musculus-440222
expression of His-tagged and GST-tagged truncated nNOS, comprising residues 1-198, in Escherichia coliMus musculus-686293
expression of His-tagged NOS oxygenase domain lacking the N-terminal 114 residues in Escherichia coliMus musculusP29477440219
expression of neuronal isoform wild type and mutants C415H, C415A in Spodoptera frugiperda cells via baculovirus infectionMus musculus-440234
expression of the C-terminally His-tagged iNOS oxygenase domain, iNOSoxy, in Escherichia coli strain BL21Mus musculus-687548
expression of the oxygenase domain of inducible nitric oxide synthase in Escherichia coliMus musculus-687291
neuronal wild-type and natural mutant, specifically expressed in the central nervous system, expression in Escherichia coli and in insect cells of Spodoptera frugiperda via baculovirus infectionMus musculus-440217
transient expression of GFP-tagged isozyme NOS1 in COS-1 cell mitochondria, N-terminally truncated GFP-tagged isozyme NOS1 is localized in the cytosolMus musculus-673662
eNOS and iNOS expression analysisRattus norvegicus-685867
expressed in HEK-293 cells; expressed in HEK-293 cellsRattus norvegicus-695853
expression of eNOS in Escherichia coliRattus norvegicus-687615
expression of GST-tagged splicing variants alpha and beta in HEK 293 cellsRattus norvegicus-686293
expression of His-tagged wild-type enzyme and untagged truncation mutant enzyme in Escherichia coli strain BL21(DE3)Rattus norvegicus-685120
expression of nNOS in Escherichia coliRattus norvegicus-687697
neuronal enzyme, stable expression in HEK-293 cellsRattus norvegicus-672363
rat brain enzyme expressed in human 293 kidney cells transfected with a vector encoding rat brain enzymeRattus norvegicus-440192, 440220
the oxygenase domain of iNOS with a C-terminal His6-tag is expressed in Escherichia coliRattus norvegicus-698484
the wild type and the DELTAG810 mutant of nNOS are cloned in Escherichia coli strain DH5alphaRattus norvegicus-698852
recombinant expression of bNOSStaphylococcus aureus-684957

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
expression of eNOS mRNA and protein does not change in mild to moderate chronic obstructive pulmonary disease, but is reduced in the more severe stages of chronic obstructive pulmonary diseaseHomo sapiens-695513
neither interleukin-1 nor tissue necrosis factor-alpha is capable of inducing nNOS synthesis for up to 48 h treatment, treatment with insulin-like growth factor-1 or transforming growth factor-beta for up to 48 h has no effect on the levels of nNOS; treatment with insulin-like growth factor-1 or transforming growth factor-beta for up to 48 h has no effect on the levels of iNOSHomo sapiens-700490
in the peripheral lung of chronic obstructive pulmonary disease patients iNOS mRNA expression is increased at earlier stages of the disease but decreases in the most severe patients, pro-inflammatory cytokine-induced iNOS expression is decreased by oxidative stress, such as H2O2, cigarette smoke conditioned media, and the peroxyntrite generator SIN1; lung tissue from patients with severe and very severe chronic obstructive pulmonary disease have graded increases in nNOS (mRNA and protein) compared with non-smokers and normal smokersHomo sapiens-695513
up-regulation of the inflammatory gene encoding for iNOS is observed in a population of amoeboid microglial cells from Parkinson's disease patients, iNOS up-regulation in microglia seems to play an important role in the onset of inflammatory processes in Parkinsons's disease and acts synergistically with other inflammatory mediators, Parkinsons's disease-inducing drugs such as rotenone and 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine or dopamine overexposure provoke activation of iNOS expressionHomo sapiens-700432
treatment with interleukin-1 or tissue necrosis factor-alpha for 16 h significantly increases iNOS levels to 429.4% of the controlHomo sapiens-700490
mitogen-activated protein kinase phosphatase-1 may negatively regulate iNOS expression by controlling the expression of microRNA-155 and consequently the signal transducer and activator of transcription pathway via suppressor of cytokine signaling-1Mus musculus-698939
exposure of primary cortical astrocytes to a low concentration of Mn2+ (0.01 mM) potentiates expression of NOS2 mRNA and proteinMus musculus-693776
expression of both the eNOS protein and gene is significantly reduced in tight-skin 1-mouse skin tissueMus musculusP70313701194
0.01 mM 2-phenyl-ethenesulfonic acid phenyl ester, 2-phenyl-ethenesulfonic acid 4-chlorophenyl ester, and 2-methyl-4-nitro-quinoline 1-oxide inhibit the expression of inducible nitric oxide synthase protein, 4-nitro-quinoline 1-oxide reveals no significant expression-downregulating activity at 0.01 mMRattus norvegicus-696643
nitric oxide synthase activity and expression are decreased in the paraventricular nucleus of pregnant ratsRattus norvegicus-697042
expression of isozyme NOS1 protein is decreased in the cytoplasmic and plasma membrane fractions, although maintained in the intracellular membrane fraction, in response to high saltRattus norvegicus-697412
0.1 mg 6-n-propyl-2-thyouracil decreases nNOS protein level to 19% compared to controlRattus norvegicusP29475700433
expression of isozyme NOS3 protein is unaffected by high saltRattus norvegicus-697412
nitric oxide synthase activity is significantly affected in ipsilateral and contralateral testes cells after vasal and epididymal ligation; nitric oxide synthase activity is significantly affected in ipsilateral and contralateral testes cells after vasal and epididymal ligation, eNOS immunoreactivity increases markedly after ipsilateral vasal ligationRattus norvegicus-695396
urothelial and endothelial i-NOS expression are significantly increased in neuromodulated rats; urothelial n-NOS expression is significantly increased in neuromodulated ratsRattus norvegicus-698343
0.001 mg thyroxin significantly increases nNOS protein level to 178% compared to controlRattus norvegicusP29475700433

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
S1179DBos taurus-the phosphomimetic substitution at Ser-1179 doubles maximal synthase activity, partially disinhibits cytochrome c reductase activity, and lowers the EC50(Ca2+) values for calmodulin binding and enzyme activation about 35%698884
S617DBos taurus-the phosphomimetic substitution doubles the maximum calmodulin-dependent enzyme activity and decreases the EC50(Ca2+) values for calmodulin binding and enzyme activation compared to the wild type enzyme696280
S617D/S1179DBos taurus-the mutation doubles maximal synthase activity, partially disinhibits cytochrome c reductase activity, and lowers the EC50(Ca2+) values for calmodulin binding and enzyme activation698884
S617D/S635DBos taurus-the maximum calmodulin-dependent synthase activity of S617/635D eNOS is about 2fold higher than the wild type activity696280
D597N/M336VHomo sapiens-mutant of nNOS, the Ki values for the nNOS double mutant increase for all inhibitors but the mutant still binds these inhibitors better than eNOS699457
E298DHomo sapiens-comparable to wild-type in heme and flavin content, in affinity to calmodulin and dimerization660022
G2AHomo sapiens-the mutant is defective in activity and cellular localization700937
C415AMus musculus-contains no heme, no bound tetrahydrobiopterin, unable to oxidize NADPH and to synthezise nitric oxide, unaltered ability to reduce cytochrome c440234
V346IMus musculusP29477slower NO-binding and dissociation than wild-type659336
W457AMus musculus-increase in Km-value of tetrahydrobiopterin and L-arginine, decrease in NO synthesis activity, slower building of enzyme heme-NO complex and slower reactivity of heme-dioxy intermediate658119
D1393ERattus norvegicus-normal composition, spectral properties and binding of cofactors, substrates and calmodulin, slower NADPH-dependent cytochrome c and ferricyanide reductase activity659330
D1393NRattus norvegicus-normal composition, spectral properties and binding of cofactors, substrates and calmodulin, slower NADPH-dependent cytochrome c and ferricyanide reductase activity659330
V567ERattus norvegicusP29476little stability, no enzymic activity659638
V567FRattus norvegicusP29476enzymic activity only with N-hydroxy-L-arginine659638
V567LRattus norvegicusP29476little production of NO, lower affinity for L-arginine and N-hydroxy-L-arginine than wild-type659638
V567RRattus norvegicusP29476little stability, no enzymic activity659638
W678ARattus norvegicus-increase in Km-value of tetrahydrobiopterin, decrease in NO synthesis activity, slower building of enzyme heme-NO complex and slower reactivity of heme-dioxy intermediate658119
W678FRattus norvegicus-increase in Km-value of tetrahydrobiopterin, decrease in NO synthesis activity, slower building of enzyme heme-NO complex and slower reactivity of heme-dioxy intermediate658119
I224VBacillus subtilis-little effect on binding of L-arginie, tetrahydrobiopterin or in electronic properties659336
additional informationBos taurus-deletion of the autoinhibitory domain doubles the maximum calmodulin-dependent enzyme activity and decreases the EC50(Ca2+) values for calmodulin binding and calmodulin-dependent enzyme activation696280
S635DBos taurus-the substitution has little or no effect on enzyme activity696280
additional informationCricetulus griseus-enhancement of nitric oxide production by association of neuronal nitric oxide synthase with N-methyl-D-aspartate receptors via postsynaptic density 95 proteins in genetically engineered Chinese hamster ovary cells, method development and quantitative analysis, overview675520
C415HMus musculus-contains nearly no heme, no bound tetrahydrobiopterin, unable to oxidize NADPH and to synthezise nitric oxide, unaltered ability to reduce cytochrome c440234
additional informationMus musculus-naturally occuring neuronal mutant with a 105-amino acid deletion in the heme-binding domain as a result of in-frame mutation by specific alternative splicing, contains heme, but shows no L-arginine and NADPH-dependent citrulline-forming activity in presence of Ca2+-promoted calmodulin440217
additional informationMus musculusP29477monomers encoding NOS oxygenase domain lacking the N-terminal 114 residues, His-tagged, expression in Escherichia coli440219
additional informationMus musculus-construction of a heterodimer with one subunit being His-tagged440221
additional informationMus musculus-generation of mice chimeric for iNOS expression, wild-type animals receive bone marrow cells from CD45.1-expressing congenic wild-type mice, resulting in three different chimeric strains, phenotype with induced colitis, overview684317
additional informationMus musculus-knock out mice deficient for the splice-isoform alpha of neuronal nitric oxide synthase display residual nitric oxide synthase activity and immunosignal686293
additional informationMus musculus-construction of eNOS and of iNOS knockout mice, phenotype, overview688600
D1393VRattus norvegicus-normal composition, spectral properties and binding of cofactors, substrates and calmodulin, slower NADPH-dependent cytochrome c and ferricyanide reductase activity659330
additional informationRattus norvegicus-mutant DELTA296nNOS, missing N-terminal leader sequence, no impact on enzyme structure or catalysis, mutant DELTA349nNOS, missing N-terminal leader sequence plus three core motifs, weaker dimer interaction, slowerferric heme production, 10fold poorer affinity towards L-arginine659257
additional informationRattus norvegicus-construction of a truncated mutant lacking the terminal half of the C-terminus, i.e. residues from Ile1413 to Ser1429. The mutation has almost no impact on NADP+ release, flavin reduction, flavin autoxidation, heme reduction, reductase activity, or NO synthesis activity, but does prevent an increase in FMN shielding that normally occurs in response to NADPH binding. Additional removal of the C-terminal alpha-helix, residues 1401 to 1412, significantly increases the NADP+ release rate, flavin autoxidation, and NADPH oxidase activity, and causes hyper-deshielding of the FMN cofactor associated with increased reductase activity and slightly diminished heme reduction and NO synthesis. Further removal of residues downstream from Gly1396, a full C-terminus truncation, amplifies the aforementioned effects and in addition altered NADP+ interaction with FAD, relieves the kinetic suppression on flavin reduction, and further diminishes heme reduction and NO synthesis685120
additional informationRattus norvegicus-knock out mice deficient for the splice-isoform alpha of neuronal nitric oxide synthase display residual nitric oxide synthase activity and immunosignal, phenotype, overview686293
additional informationRattus norvegicus-the DELTAG810 mutant enzyme shows strongly reduced activity towards nitric oxide synthesis and NADPH oxidation compared to the wild type enzyme698852

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
refolding after treatment/equilibration with 5 M urea in presence of L-arginine and tetrahydrobiopterinMus musculus-440238

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
medicineEntamoeba histolytica-enzyme induces relaxation of endothelium-denuded rat aortic rings precontracted with phenylepinephrine, possible participation in pathogenesis of invasive amebiasis658664
medicineHomo sapiens-no evidence for altered enzyme function of mutant E298D that could explain endothelial dysfunction associated with the E298D polymorphism660022
pharmacologyHomo sapiens-NO synthase can be used to gain insight into the biological role of endogenous agmatine440233
medicineOryctolagus cuniculus-nitric oxide synthase inhibitors, e.g. L-N(G)-nitro-arginine-methylester, may serve as an alternative adjunct with antibiotic therapy for Pseudomonas keratitis, especially for reducing ocular inflammation without affecting the antimicrobial efficacy of the antibiotic661659

DISEASETITLE OF PUBLICATIONLINK TO PUBMED
Aberrant Crypt FociA 116-kDa phytoglycoprotein inhibits aberrant crypt foci formation through modulation of manganese superoxide dismutase, inducible nitric oxide synthase, cyclooxygenase-2, nuclear factor-kappa B, activator protein-1, and proliferating cell nuclear antigen in 1,2-dimethylhydrazine/dextran sodium sulfate-treated ICR mice. PubMed
Aberrant Crypt FociApigenin and naringenin suppress colon carcinogenesis through the aberrant crypt stage in azoxymethane-treated rats. PubMed
Aberrant Crypt FociChemopreventive potential of diallylsulfide, lycopene and theaflavin during chemically induced colon carcinogenesis in rat colon through modulation of cyclooxygenase-2 and inducible nitric oxide synthase pathways. PubMed
Aberrant Crypt FociCoenzyme Q10 attenuated DMH-induced precancerous lesions in SD rats. PubMed
Aberrant Crypt FociHigh fat diet elevates the activity of inducible nitric oxide synthase and 1,2-dimethylhydrazine-induced aberrant crypt foci in colon of rats. PubMed
Aberrant Crypt FociInducible nitric oxide synthase (iNOS) is expressed similarly in multiple aberrant crypt foci and colorectal tumors from the same patients. PubMed
Aberrant Crypt FociMulti-Target Approaches in Colon Cancer Chemoprevention Based on Systems Biology of Tumor Cell-Signaling. PubMed
Aberrant Crypt FociRole of inducible nitric oxide synthase expression in aberrant crypt foci-adenoma-carcinoma sequence. PubMed
Aberrant Crypt FociSuppression of azoxymethane-induced colonic aberrant crypt foci by a nitric oxide synthase inhibitor. PubMed
Abortion, HabitualAltered endometrial expression of endothelial nitric oxide synthase in women with unexplained recurrent miscarriage and infertility. PubMed
Abortion, HabitualEndothelial nitric oxide synthase gene polymorphism in women with idiopathic recurrent miscarriage. PubMed
Abortion, HabitualGenetic analysis of eNOS gene polymorphisms in association with recurrent miscarriage among North Indian women. PubMed
Abortion, HabitualPolymorphisms of the angiotensinogen gene, the endothelial nitric oxide synthase gene, and the interleukin-1beta gene promoter in women with idiopathic recurrent miscarriage. PubMed
Abortion, SpontaneousEndothelial nitric oxide synthase gene polymorphisms (-786T>C, 4a4b, 894G>T) and haplotypes in Korean patients with recurrent spontaneous abortion. PubMed
Abortion, SpontaneousEndothelial nitric oxide synthase gene polymorphisms in recurrent spontaneous abortions. PubMed
Abortion, SpontaneousExpression of the inducible nitric oxide synthase isoform in chorionic villi in the early spontaneous abortion. PubMed
Abortion, SpontaneousProduction of NO and oxidative destruction of proteins in the placenta during normal pregnancy and placental insufficiency. PubMed
Abortion, SpontaneousPsychiatric morbidity following miscarriage: a prevalence study of Chinese women in Hong Kong. PubMed
Abruptio PlacentaeEndothelial nitric oxide synthase polymorphism is not associated with placental abruption in Finnish women. PubMed
Abruptio PlacentaeGeneration of peroxynitrite and apoptosis in placenta of patients with chorioamnionitis: possible implications in placental abruption. PubMed
Abruptio PlacentaeThe Glu298Asp variant of the endothelial nitric oxide synthase gene is associated with an increased risk for abruptio placentae in pre-eclampsia. PubMed
Abruptio PlacentaeThe missense Glu298Asp variant of the endothelial nitric oxide synthase gene is strongly associated with placental abruption. PubMed
AbscessCD14 works with toll-like receptor 2 to contribute to recognition and control of Listeria monocytogenes infection. PubMed
AbscessIntracerebroventricular injection of neuronal and inducible nitric oxide synthase inhibitors does not influence febrile response in rats during turpentine abscess. PubMed
AbscessProtective effects of N(G)-nitro-L-arginine methyl ester and metallothioneins on excess nitric oxide toxicity in trinitrobenzene sulfonic acid-induced rat colitis. PubMed
Acalculous Cholecystitis[The role of stomach epithelial cells being immunopositive for NO synthase in the formation of chronic cholecystitis] PubMed
AcantholysisThe role of nitric oxide synthases in pemphigus vulgaris in a mouse model. PubMed
AcidosisBeneficial effect of carboxy-PTIO on hemodynamic and blood gas changes in septic shock dogs. PubMed
AcidosisEffects of hypercapnia and NO synthase inhibition in sustained hypoxic pulmonary vasoconstriction. PubMed
AcidosisEvaluation of the role of nitric oxide in acid sensing ion channel mediated cell death. PubMed
AcidosisHypercapnia relaxes cerebral arteries and potentiates neurally-induced relaxation. PubMed
AcidosisInfluence of the glia limitans on pial arteriolar relaxation in the rat. PubMed
AcidosisIs intracellular brain pH a dependent factor in NOS inhibition during focal cerebral ischemia? PubMed
AcidosisIs neuroprotective efficacy of nNOS inhibitor 7-NI dependent on ischemic intracellular pH? PubMed
AcidosisMechanisms of inducible nitric oxide synthase (iNOS) inhibition-related improvement of gut mucosal acidosis during hyperdynamic porcine endotoxemia. PubMed
AcidosisNitric oxide from perivascular nerves modulates cerebral arterial pH reactivity. PubMed
AcidosisNitric oxide synthase inhibition does not affect the exercise-induced arterial hypoxemia in Thoroughbred horses. PubMed
AcidosisNo effect of metabolic acidosis on nitric oxide production in hypoxic and hyperoxic lung regions in pigs. PubMed
AcidosisProlonged hypercapnia-evoked cerebral hyperemia via K(+) channel- and prostaglandin E(2)-dependent endothelial nitric oxide synthase induction. PubMed
AcidosisResponse of intra-acinar pulmonary microvessels to hypoxia, hypercapnic acidosis, and isocapnic acidosis. PubMed
AcidosisRole of iNOS and eNOS in modulating proximal tubule transport and acid-base balance. PubMed
AcidosisRole of nitric oxide in acidosis-induced intestinal injury in anesthetized rats. PubMed
Acidosis, LacticA selective inhibitor for inducible nitric oxide synthase improves hypotension and lactic acidosis in canine endotoxic shock. PubMed
Acquired Immunodeficiency SyndromeCharacterization and regional distribution of nitric oxide synthase in the human brain during normal ageing. PubMed
Acquired Immunodeficiency SyndromeExpression of inducible nitric oxide synthase in cytomegalovirus-infected glial cells of retinas from AIDS patients. PubMed
Acquired Immunodeficiency SyndromeExpression of inducible nitric oxide synthase, interleukin-1 and caspase-1 in HIV-1 encephalitis. PubMed
Acquired Immunodeficiency SyndromeHIV-1 coat protein gp120 decreases NO-dependent cyclic GMP accumulation in rat brain astroglia by increasing cyclic GMP phosphodiesterase activity. PubMed
Acquired Immunodeficiency SyndromeHuman immunodeficiency virus infection, inducible nitric oxide synthase expression, and microglial activation: pathogenetic relationship to the acquired immunodeficiency syndrome dementia complex. PubMed
Acquired Immunodeficiency SyndromeHuman immunodeficiency virus-1-infected macrophages induce inducible nitric oxide synthase and nitric oxide (NO) production in astrocytes: astrocytic NO as a possible mediator of neural damage in acquired immunodeficiency syndrome. PubMed
Acquired Immunodeficiency SyndromeImmunologic NO synthase: elevation in severe AIDS dementia and induction by HIV-1 gp41. PubMed
Acquired Immunodeficiency SyndromeIncreased NADPH-diaphorase reactivity and cytokine expression in dorsal root ganglia in acquired immunodeficiency syndrome. PubMed
Acquired Immunodeficiency SyndromeIntensity of myocardial expression of inducible nitric oxide synthase influences the clinical course of human immunodeficiency virus-associated cardiomyopathy. Gruppo Italiano per lo Studio Cardiologico dei pazienti affetti da AIDS (GISCA). PubMed
Acquired Immunodeficiency SyndromeNitric oxide synthase expression and apoptotic cell death in brains of AIDS and AIDS dementia patients. PubMed
Acquired Immunodeficiency SyndromeProtein kinase C regulates NO-cGMP pathway in muscarinic receptor activation by HIV+-IgA. PubMed
Acquired Immunodeficiency SyndromeRegulation of nitric oxide synthase activity in human immunodeficiency virus type 1 (HIV-1)-infected monocytes: implications for HIV-associated neurological disease. PubMed
AcrospiromaValue of p63 and podoplanin (D2-40) immunoreactivity in the distinction between primary cutaneous tumors and adenocarcinomas metastatic to the skin: a clinicopathologic and immunohistochemical study of 79 cases. PubMed
ACTH Syndrome, EctopicCushing's syndrome caused by an ACTH-producing ovarian steroid cell tumor, NOS, in a prepubertal girl. PubMed
ACTH-Secreting Pituitary AdenomaNeuronal nitric oxide synthase gene expression in human pituitary tumours: a possible association with somatotroph adenomas and growth hormone-releasing hormone gene expression. PubMed
Acute Chest SyndromeLow exhaled nitric oxide and a polymorphism in the NOS I gene is associated with acute chest syndrome. PubMed
Acute Chest SyndromePhysician-diagnosed asthma and acute chest syndrome: associations with NOS polymorphisms. PubMed
Acute Coronary SyndromeAllelic polymorphism in the promoter (T-->C), but not in exon 7 (G-->T) or the variable number tandem repeat in intron 4, of the endothelial nitric oxide synthase gene is positively associated with acute coronary syndrome in the Ukrainian population. PubMed
Acute Coronary SyndromeAssociation of endothelial nitric oxide synthase promoter region (T-786C) gene polymorphism with acute coronary syndrome and coronary heart disease. PubMed
Acute Coronary SyndromeInfluence of endothelial nitric oxide synthase gene polymorphisms (G894T, 4a4b, T-786C) and hyperhomocysteinemia on the predisposition to acute coronary syndromes. PubMed
Acute Coronary SyndromeInteraction between endothelial nitric oxide synthase gene polymorphisms (-786T>C, 894G>T and intron 4 a/b) and cardiovascular risk factors in acute coronary syndromes. PubMed
Acute Coronary SyndromeInteraction of CA repeat polymorphism of the endothelial nitric oxide synthase and hyperhomocysteinemia in acute coronary syndromes: evidence of gender-specific differences. PubMed
Acute Coronary SyndromeSerum neopterin, nitric oxide, inducible nitric oxide synthase and tumor necrosis factor-alpha levels in patients with ischemic heart disease. PubMed
Acute Coronary Syndrome[Association of endothelial nitric oxide synthase gene polymorphism with level of uric acid in serum for acute coronary syndrome] PubMed
Acute Kidney InjuryAltered expression of endothelin-1 and endothelial nitric oxide synthase in the juxtaglomerular apparatus of rats with HgCl2-induced acute renal failure. PubMed
Acute Kidney InjuryDocosahexaenoic acid ameliorates murine ischemic acute renal failure and prevents increases in mRNA abundance for both TNF-alpha and inducible nitric oxide synthase. PubMed
Acute Kidney InjuryEffect of erythropoietin therapy on the progression of cisplatin induced renal injury in rats. PubMed
Acute Kidney InjuryEffect of selective inhibition of renal inducible nitric oxide synthase on renal blood flow and function in experimental hyperdynamic sepsis. PubMed
Acute Kidney InjuryEndothelial nitric oxide contributes to the renal protective effects of ischemic preconditioning. PubMed
Acute Kidney InjuryEndotoxin-induced acute renal failure in rats: effects of L-arginine and nitric oxide synthase inhibition on renal function. PubMed
Acute Kidney InjuryErythropoietin prevents sepsis-related acute kidney injury in rats by inhibiting nuclear-factor kappa B and upregulating endothelial nitric oxide synthase. PubMed
Acute Kidney InjuryExpression profile of a human inducible nitric oxide synthase promoter reporter in transgenic mice during endotoxemia. PubMed
Acute Kidney InjuryIn vitro model of sepsis-induced renal epithelial reactive nitrogen species generation. PubMed
Acute Kidney InjuryIn vivo targeting of inducible NO synthase with oligodeoxynucleotides protects rat kidney against ischemia. PubMed
Acute Kidney InjuryIncreased nitric oxide synthase activity despite lack of response to endothelium-dependent vasodilators in postischemic acute renal failure in rats. PubMed
Acute Kidney InjuryL-arginine as a therapeutic tool in kidney disease. PubMed
Acute Kidney InjuryL-Arginine transport is augmented through up-regulation of tubular CAT-2 mRNA in ischemic acute renal failure in rats. PubMed
Acute Kidney InjuryMagnesium supplementation combined with N-acetylcysteine protects against postischemic acute renal failure. PubMed
Acute Kidney InjuryNitric oxide in acute renal failure: NOS versus NOS. PubMed
Acute Kidney InjuryNitric oxide in the regulation of renal blood flow. PubMed
Acute Kidney InjuryPlasma protein extravasation and vascular endothelial growth factor expression with endothelial nitric oxide synthase induction in gentamicin-induced acute renal failure in rats. PubMed
Acute Kidney InjuryProtective effect of nitric oxide on ischemia/reperfusion-induced renal injury and endothelin-1 overproduction. PubMed
Acute Kidney InjuryRenal endothelial dysfunction and impaired autoregulation after ischemia-reperfusion injury result from excess nitric oxide. PubMed
Acute Kidney InjuryRole of angiotensin II, endothelin-1, and nitric oxide in HgCl2-induced acute renal failure. PubMed
Acute Kidney InjuryRole of nitric oxide synthase activity in experimental ischemic acute renal failure in rats. PubMed
Acute Kidney InjuryRole of sepiapterin on endothelial nitric oxide synthase in acute kidney injury: an enigmatic story. PubMed
Acute Kidney InjurySesame oil prevents acute kidney injury induced by the synergistic action of aminoglycoside and iodinated contrast in rats. PubMed
Acute Kidney Injury[Role of nitric oxide system in pathogenesis of experimental model of hepatorenal syndrome] PubMed
Acute Lung InjuryActions of NO and INOS on endotoxin induced rat acute lung injury and effect of rhubarb on them. PubMed
Acute Lung InjuryActivation of the stress protein response inhibits the STAT1 signalling pathway and iNOS function in alveolar macrophages: role of Hsp90 and Hsp70. PubMed
Acute Lung InjuryAcute pancreatitis: an overview of emerging pharmacotherapy. PubMed
Acute Lung InjuryAcute respiratory distress syndrome. PubMed
Acute Lung InjuryAminoguanidine attenuates endotoxin-induced acute lung injury in rabbits. PubMed
Acute Lung InjuryAn inhaled inducible nitric oxide synthase inhibitor reduces damage of Candida-induced acute lung injury. PubMed
Acute Lung InjuryAnti-inflammatory and antioxidant effects of infliximab on acute lung injury in a rat model of intestinal ischemia/reperfusion. PubMed
Acute Lung InjuryAttenuated expression of inducible nitric oxide synthase in lung microvascular endothelial cells is associated with an increase in ICAM-1 expression. PubMed
Acute Lung InjuryCombined neuronal and inducible nitric oxide synthase inhibition in ovine acute lung injury. PubMed
Acute Lung InjuryDexamethasone attenuated endotoxin-induced acute lung injury through inhibiting expression of inducible nitric oxide synthase. PubMed
Acute Lung InjuryDifferential effects of nitric oxide synthesis on pulmonary vascular function during lung ischemia-reperfusion injury. PubMed
Acute Lung InjuryDirect delivery of low-dose 7-nitroindazole into the bronchial artery attenuates pulmonary pathophysiology after smoke inhalation and burn injury in an ovine model. PubMed
Acute Lung InjuryDual Peptide Nucleic Acid- and Peptide-Functionalized Shell Cross-Linked Nanoparticles Designed to Target mRNA toward the Diagnosis and Treatment of Acute Lung Injury. PubMed
Acute Lung InjuryEffect of aminoguanidine on lung fluid filtration after endotoxin in awake sheep. PubMed
Acute Lung InjuryEffect of inducible nitric oxide synthase on apoptosis in Candida-induced acute lung injury. PubMed
Acute Lung InjuryEffects of inhaled NO and inhibition of endogenous NO synthesis in oxidant-induced acute lung injury. PubMed
Acute Lung InjuryEffects of macrophage inducible nitric oxide synthase in murine septic lung injury. PubMed
Acute Lung InjuryEffects of nitric oxide synthase inhibitors on systemic hypotension, cytokines and inducible nitric oxide synthase expression and lung injury following endotoxin administration in rats. PubMed
Acute Lung InjuryEndothelin receptor blockade attenuates lipopolysaccharide-induced pulmonary nitric oxide production. PubMed
Acute Lung InjuryExpression of nitric oxide synthase, aquaporin 1 and aquaporin 5 in rat after bleomycin inhalation. PubMed
Acute Lung InjuryGlycyrrhizin treatment is associated with attenuation of lipopolysaccharide-induced acute lung injury by inhibiting cyclooxygenase-2 and inducible nitric oxide synthase expression. PubMed
Acute Lung InjuryInducible nitric oxide synthase contributes to ventilator-induced lung injury. PubMed
Acute Lung InjuryInhibition of inducible nitric oxide synthase prevents LPS-induced acute lung injury in dogs. PubMed
Acute Lung InjuryInhibition of neuronal nitric oxide synthase by 7-nitroindazole attenuates acute lung injury in an ovine model. PubMed
Acute Lung InjuryInhibition of neuronal nitric oxide synthase in ovine model of acute lung injury. PubMed
Acute Lung InjuryInhibition of nitric oxide synthase attenuates NNMU-induced alveolar injury in vivo. PubMed
Acute Lung InjuryMechanistic aspects of inducible nitric oxide synthase-induced lung injury in burn trauma. PubMed
Acute Lung InjuryModulation of proinflammatory cytokines by nitric oxide in murine acute lung injury. PubMed
Acute Lung InjuryMolecular biological effects of selective neuronal nitric oxide synthase inhibition in ovine lung injury. PubMed
Acute Lung InjuryNitric oxide in the cardiovascular and pulmonary circulation--a brief review of literatures and historical landmarks. PubMed
Acute Lung InjuryONO-1714, a nitric oxide synthase inhibitor, attenuates endotoxin-induced acute lung injury in rabbits. PubMed
Acute Lung InjuryOsteopontin is strongly expressed by alveolar macrophages in the lungs of acute respiratory distress syndrome. PubMed
Acute Lung InjuryProtective effect of curcumin on acute lung injury induced by intestinal ischemia/reperfusion. PubMed
Acute Lung InjuryPulmonary neutrophil infiltration in murine sepsis: role of inducible nitric oxide synthase. PubMed
Acute Lung InjuryRole of different nitric oxide synthase isoforms in a murine model of acute lung injury and sepsis. PubMed
Acute Lung InjuryRole of inducible nitric oxide synthase expressed by alveolar macrophages in high mobility group box 1--induced acute lung injury. PubMed
Acute Lung InjuryRole of inducible nitric oxide synthase in endotoxin-induced acute lung injury. PubMed
Acute Lung InjuryRole of inducible nitric oxide synthase in pulmonary microvascular protein leak in murine sepsis. PubMed
Acute Lung InjuryRuscogenin inhibits lipopolysaccharide-induced acute lung injury in mice: involvement of tissue factor, inducible NO synthase and nuclear factor (NF)-?B. PubMed
Acute Lung InjuryS-nitroso human serum albumin given after LPS challenge reduces acute lung injury and prolongs survival in a rat model of endotoxemia. PubMed
Acute Lung InjuryS-nitroso-human-albumin: a new therapeutic approach in endotoxic shock. PubMed
Acute Lung InjurySelective inducible nitric oxide synthase (iNOS) inhibition attenuates remote acute lung injury in a model of ruptured abdominal aortic aneurysm. PubMed
Acute Lung InjurySepsis and hyperoxia effects on the pulmonary surfactant system in wild-type and iNOS knockout mice. PubMed
Acute Lung InjurySpecific Role of Neutrophil Inducible Nitric Oxide Synthase in Murine Sepsis-Induced LungInjury in vivo (104 characters). PubMed
Acute Lung InjuryThe inducible nitric oxide synthase inhibitor BBS-2 prevents acute lung injury in sheep after burn and smoke inhalation injury. PubMed
Acute Lung InjuryTreatment of sepsis-induced acquired protein C deficiency reverses Angiotensin-converting enzyme-2 inhibition and decreases pulmonary inflammatory response. PubMed
Acute Lung InjuryVentilator-induced lung injury is reduced in transgenic mice that overexpress endothelial nitric oxide synthase. PubMed
Acute Lung Injury[Expression NOS in acute lung injury following limb ischemia/reprefusion and its significance in rats]. PubMed
Acute Lung Injury[The effect of nitric oxide on acute lung injury following ischemia/reperfusion of hind limbs in the rat] PubMed
Acute PainInhibition of nitric oxide synthase enhances antinociception mediated by mu, delta and kappa opioid receptors in acute and prolonged pain in the rat spinal cord. PubMed
AdenocarcinomaA novel model system for studying the double-edged roles of nitric oxide production in pancreatic cancer growth and metastasis. PubMed
AdenocarcinomaAltered expression of beta-catenin, inducible nitric oxide synthase and cyclooxygenase-2 in azoxymethane-induced rat colon carcinogenesis. PubMed
AdenocarcinomaAngiogenic markers, neovascularization and malignant deformation of Barrett's esophagus. PubMed
AdenocarcinomaBioassay of 2,4-diaminoanisole sulfate for possible carcinogenicity. PubMed
AdenocarcinomaBioassay of sulfallate for possible carcinogenicity (CAS No. 95-06-7). PubMed
AdenocarcinomaCancers of the ampulla of vater: Demographics, morphology, and survival based on 5,625 cases from the SEER program. PubMed
AdenocarcinomaCarcinoma in basal cell adenoma of the parotid gland. PubMed
AdenocarcinomaCarcinoma NOS is a Common Histologic Diagnosis and is Increasing in Proportion Among Non-small Cell Lung Cancer Histologies. PubMed
AdenocarcinomaCarcinoma of the parotid and submandibular glands--a study of survival in 2465 patients. PubMed
AdenocarcinomaCeruminous gland carcinomas: a clinicopathologic and immunophenotypic study of 17 cases. PubMed
AdenocarcinomaClinical prognostic factors in malignant parotid gland tumors. PubMed
AdenocarcinomaCloning and functional expression of human inducible nitric oxide synthase (NOS) cDNA from a glioblastoma cell line A-172. PubMed
AdenocarcinomaColonic adenocarcinomas rapidly induced by the combined treatment with 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine and dextran sodium sulfate in male ICR mice possess beta-catenin gene mutations and increases immunoreactivity for beta-catenin, cyclooxygenase-2 and inducible nitric oxide synthase. PubMed
AdenocarcinomaColorectal cancer in iran: an epidemiological study. PubMed
AdenocarcinomaCombination of atorvastatin with sulindac or naproxen profoundly inhibits colonic adenocarcinomas by suppressing the p65/beta-catenin/cyclin D1 signaling pathway in rats. PubMed
AdenocarcinomaCorrelation Between Inducible Nitric Oxide Synthase and Cyclooxygenase-2 Expression in Human Colorectal Adenocarcinoma: A Cross-Sectional Study. PubMed
AdenocarcinomaCyclooxygenase-2 and Inducible Nitric Oxide Synthase Gene Polymorphisms and Risk of Reflux Esophagitis, Barrett's Esophagus, and Esophageal Adenocarcinoma. PubMed
AdenocarcinomaCytokines stimulate expression of inducible nitric oxide synthase in DLD-1 human adenocarcinoma cells by activating poly(A) polymerase. PubMed
AdenocarcinomaCytologic features of adenocarcinoma, not otherwise specified, in conventional smears: comparison of cases that performed poorly with those that performed well in the College of American Pathologists Interlaboratory Comparison Program in cervicovaginal cytology. PubMed
AdenocarcinomaDifferent expression patterns of nitric oxide synthase isozymes in various gynecological cancers. PubMed
AdenocarcinomaDifferential expression of nitric oxide synthase in human stomach cancer. PubMed
AdenocarcinomaDifferential regulation of inducible nitric oxide synthase gene expression by ethanol in the human intestinal epithelial cell line DLD-1. PubMed
AdenocarcinomaEffects of Feiyanning Decoction, a compound traditional Chinese medicine, on iNOS and COX-2 expressions induced by tumor necrosis factor-? in lung adenocarcinoma cell line. PubMed
AdenocarcinomaEndoscopic ultrasound guided fine needle aspiration biopsy of autoimmune pancreatitis: diagnostic criteria and pitfalls. PubMed
AdenocarcinomaEpstein-Barr Virus and Breast Cancer: Lack of Evidence for an Association in Iranian Women. PubMed
AdenocarcinomaEstimated median survival times of patients with colorectal cancer based on experience with 9,745 patients. PubMed
AdenocarcinomaExpression of cytokeratin 20 in mucinous bronchioloalveolar carcinoma. PubMed
AdenocarcinomaExpression of E-cadherin, cellular differentiation and polarity in epithelial salivary neoplasms. PubMed
AdenocarcinomaExpression of inducible nitric oxide synthase and cyclooxygenase-2 in pancreatic adenocarcinoma: correlation with microvessel density. PubMed
AdenocarcinomaExpression of inducible nitric oxide synthase in healthy pleura and in malignant mesothelioma. PubMed
AdenocarcinomaExpression of NOS and VEGF in feline mammary tumours and their correlation with angiogenesis. PubMed
AdenocarcinomaFine needle aspiration biopsy of salivary gland lesions. A reappraisal of pitfalls and problems. PubMed
AdenocarcinomaGenetic ablation of inducible nitric oxide synthase decreases mouse lung tumorigenesis. PubMed
AdenocarcinomaGenetic disruption of host interferon-gamma drastically enhances the metastasis of pancreatic adenocarcinoma through impaired expression of inducible nitric oxide synthase. PubMed
AdenocarcinomaHeme oxygenase-1 induction by endogenous nitric oxide: influence of intracellular glutathione. PubMed
AdenocarcinomaHuman kallikrein 8 expression in salivary gland tumors. PubMed
AdenocarcinomaImmunohistochemical evaluation of the Ca(2+)-binding S-100 proteins S-100A1, S-100A2, S-100A4, S-100A6 and S-100B in salivary gland tumors. PubMed
AdenocarcinomaImmunohistochemical expression of androgen receptor in salivary gland cancers. PubMed
AdenocarcinomaImmunohistochemical study of c-erbB-2 expression in carcinoma ex-pleomorphic adenoma. PubMed
AdenocarcinomaIncreased expression of cyclooxygenase-2 and nitric oxide synthase-2 in human prostate cancer. PubMed
AdenocarcinomaIncreased expression of inducible nitric oxide synthase and cyclooxygenase-2 in Barrett's esophagus and associated adenocarcinomas. PubMed
AdenocarcinomaIncreased oxidative DNA damage, inducible nitric oxide synthase, nuclear factor kappaB expression and enhanced antiapoptosis-related proteins in Helicobacter pylori-infected non-cardiac gastric adenocarcinoma. PubMed
AdenocarcinomaIncreased risk of intestinal type of gastric adenocarcinoma in Japanese women associated with long forms of CCTTT pentanucleotide repeat in the inducible nitric oxide synthase promoter. PubMed
AdenocarcinomaInducible nitric oxide synthase, nitrotyrosine and apoptosis in gastric adenocarcinomas and their correlation with a poor survival. PubMed
AdenocarcinomaInducible nitric oxide synthase, nitrotyrosine and p53 mutations in the molecular pathogenesis of Barrett's esophagus and esophageal adenocarcinoma. PubMed
AdenocarcinomaInduction of inducible nitric oxide synthase: a protective mechanism in colitis-induced adenocarcinoma. PubMed
AdenocarcinomaInhibition of gastric cancer cell proliferation by resveratrol: role of nitric oxide. PubMed
AdenocarcinomaiNOS initiates and sustains metabolic arrest in hypoxic lung adenocarcinoma cells: mechanism of cell survival in solid tumor core. PubMed
AdenocarcinomaLip cancer experience in Mexico. An 11-year retrospective study. PubMed
AdenocarcinomaMalignant salivary gland tumors and cyclo-oxygenase-2: A histopathological and immunohistochemical analysis with implications on histogenesis. PubMed
AdenocarcinomaMolecular characterization of salivary gland malignancy using the Smgb-Tag transgenic mouse model. PubMed
AdenocarcinomaMorphology, TNM Staging and Survival with Pancreatico-duodenectomy Specimens received at Shaukat Khanum Memorial Cancer Hospital and Research Centre, Pakistan. PubMed
AdenocarcinomaMucinous differentiation in colonic adenocarcinoma is associated with a reduction in tumour-infiltrating lymphocytes. PubMed
AdenocarcinomaNitric oxide promotes murine mammary tumour growth and metastasis by stimulating tumour cell migration, invasiveness and angiogenesis. PubMed
AdenocarcinomaNitric oxide synthase activity and expression in human colorectal cancer. PubMed
AdenocarcinomaNitric oxide synthase activity in human lung cancer. PubMed
AdenocarcinomaNitric oxide synthase as a marker in colorectal carcinoma. PubMed
AdenocarcinomaNitric oxide synthase expression and enzymatic activity in human brain tumors. PubMed
AdenocarcinomaNon-hematopoietic cutaneous metastases in children and adolescents: thirty years experience at St. Jude Children's Research Hospital. PubMed
AdenocarcinomaNonsmall cell lung carcinoma with neuroendocrine differentiation--an entity of no clinical or prognostic significance. PubMed
AdenocarcinomaOutcome of special types of luminal breast cancer. PubMed
AdenocarcinomaOverexpression of endothelium-derived nitric oxide synthase isoform 3 in the vasculature of human pancreatic tumor biopsies. PubMed
AdenocarcinomaPhenotypic diversity of intrahepatic and extrahepatic cholangiocarcinoma on aspiration cytology and core needle biopsy: case series and review of the literature. PubMed
AdenocarcinomaProstate marker immunoreactivity in salivary gland neoplasms. A rare pitfall in immunohistochemistry. PubMed
AdenocarcinomaRegulation of interleukin-8 expression by nitric oxide in human pancreatic adenocarcinoma. PubMed
AdenocarcinomaRelative frequency of intra-oral minor salivary gland tumors: a study of 380 cases from northern California and comparison to reports from other parts of the world. PubMed
AdenocarcinomaRole of calcium in the activation of mouse peritoneal macrophages: induction of NO synthase by calcium ionophores and thapsigargin. PubMed
AdenocarcinomaS-100 protein in salivary gland tumors: an immunohistochemical study of 129 cases. PubMed
AdenocarcinomaS-Nitrosation regulates the activation of endogenous procaspase-9 in HT-29 human colon carcinoma cells. PubMed
AdenocarcinomaSalivary duct carcinoma--a clinicopathologic study of 12 cases. PubMed
AdenocarcinomaSalivary gland carcinoma in Denmark 1990-2005: a national study of incidence, site and histology. Results of the Danish Head and Neck Cancer Group (DAHANCA). PubMed
AdenocarcinomaSignificant correlation of nitric oxide synthase activity and p53 gene mutation in stage I lung adenocarcinoma. PubMed
AdenocarcinomaStimulation of signal transducer and activator of transcription-1 (STAT1)-dependent gene transcription by lipopolysaccharide and interferon-gamma is regulated by mammalian target of rapamycin. PubMed
AdenocarcinomaStudies of iron deposits, inducible nitric oxide synthase and nitrotyrosine in a rat model for esophageal adenocarcinoma. PubMed
AdenocarcinomaThe expression of cyclooxygenase-1 and -2 in proliferative endometrium and endometrial adenocarcinoma. PubMed
AdenocarcinomaThe expression of nitric oxide synthases in human brain tumours and peritumoral areas. PubMed
AdenocarcinomaThe significance of the pathologic findings in endometrial cancer. PubMed
AdenocarcinomaTubular carcinoma of the breast. A long term follow-up. PubMed
AdenocarcinomaTumour overexpression of inducible nitric oxide synthase (iNOS) increases angiogenesis and may modulate the anti-tumour effects of the vascular disrupting agent ZD6126. PubMed
AdenocarcinomaUpper lip malignant neoplasms. A study of 59 cases. PubMed
AdenocarcinomaVEGF-A and i-NOS expression are prognostic factors in serous epithelial ovarian carcinomas after complete surgical resection. PubMed
Adenocarcinoma[Carcinoma of the colon in Iceland 1955-1989. A study on pathology.] PubMed
Adenocarcinoma, MucinousEpstein-Barr Virus and Breast Cancer: Lack of Evidence for an Association in Iranian Women. PubMed
Adenocarcinoma, MucinousPrognostic factors in primary mucinous breast carcinoma. PubMed
Adenocarcinoma, MucinousThe significance of the pathologic findings in endometrial cancer. PubMed
Adenocarcinoma, MucinousTumor characteristics and clinical outcome of tubular and mucinous breast carcinomas. PubMed
Adenocarcinoma, Mucinous[Carcinoma of the colon in Iceland 1955-1989. A study on pathology.] PubMed
Adenocarcinoma, PapillaryBioassay of 2,4-diaminoanisole sulfate for possible carcinogenicity. PubMed
Adenolymphoma[Peripheral T-cell lymphoma, NOS arising in Warthin tumor: report of a case] PubMed
AdenomaAssociation of Ets-related transcriptional factor E1AF expression with overexpression of matrix metalloproteinases, COX-2 and iNOS in the early stage of colorectal carcinogenesis. PubMed
AdenomaBioassay of p-Anisidine Hydrochloride for Possible Carcinogenicity (CAS No. 20265-97-8). PubMed
AdenomaBioassay of sulfallate for possible carcinogenicity (CAS No. 95-06-7). PubMed
AdenomaCyclooxygenase-2 and inducible nitric oxide synthase expression in thyroid neoplasms and their clinicopathological correlation. PubMed
AdenomaDifferential effects of reactive nitrogen species on DNA base excision repair initiated by the alkyladenine DNA glycosylase. PubMed
AdenomaExpression of inducible nitric oxide synthase in thyroid neoplasms: immunohistochemical and molecular analysis. PubMed
AdenomaFine needle aspiration biopsy of salivary gland lesions. A reappraisal of pitfalls and problems. PubMed
AdenomaFrequent nitric oxide synthase-2 expression in human colon adenomas: implication for tumor angiogenesis and colon cancer progression. PubMed
AdenomaImmunohistochemical evaluation of the Ca(2+)-binding S-100 proteins S-100A1, S-100A2, S-100A4, S-100A6 and S-100B in salivary gland tumors. PubMed
AdenomaNeuronal nitric oxide synthase gene expression in human pituitary tumours: a possible association with somatotroph adenomas and growth hormone-releasing hormone gene expression. PubMed
AdenomaNitric oxide stimulates growth hormone secretion from human fetal pituitaries and cultured pituitary adenomas. PubMed
AdenomaNitric oxide synthase as a marker in colorectal carcinoma. PubMed
AdenomaNitric Oxide Synthase in Human Parathyroid Glands and Parathyroid Adenomas. PubMed
AdenomaNitric oxide synthase in the human pituitary gland. PubMed
AdenomaRole of inducible nitric oxide synthase expression in aberrant crypt foci-adenoma-carcinoma sequence. PubMed
AdenomaSuppression of intestinal polyposis in Apc(Min/+) mice by inhibiting nitric oxide production. PubMed
AdenomaUp-regulation of cyclooxygenase-2 expression in lymphocytic thyroiditis and thyroid tumors: significant correlation with inducible nitric oxide synthase. PubMed
Adenoma[The expression of keratins Nos. 8, 17 and vimentin in pleomorphic adenoma of the salivary glands] PubMed
Adenoma, PleomorphicCarcinoma in basal cell adenoma of the parotid gland. PubMed
Adenoma, PleomorphicExpression of E-cadherin, cellular differentiation and polarity in epithelial salivary neoplasms. PubMed
Adenoma, PleomorphicEzrin and inducible nitric oxide synthase in malignant proliferation of salivary gland pleomorphic adenoma. PubMed
Adenoma, PleomorphicFine needle aspiration biopsy of salivary gland lesions. A reappraisal of pitfalls and problems. PubMed
Adenoma, PleomorphicHuman kallikrein 8 expression in salivary gland tumors. PubMed
Adenoma, PleomorphicImmunohistochemical evaluation of the Ca(2+)-binding S-100 proteins S-100A1, S-100A2, S-100A4, S-100A6 and S-100B in salivary gland tumors. PubMed
Adenoma, PleomorphicImmunohistochemical expression of androgen receptor in salivary gland cancers. PubMed
Adenoma, PleomorphicMolecular characterization of salivary gland malignancy using the Smgb-Tag transgenic mouse model. PubMed
Adenoma, PleomorphicS-100 protein in salivary gland tumors: an immunohistochemical study of 129 cases. PubMed
Adenoma, Pleomorphic[The expression of keratins Nos. 8, 17 and vimentin in pleomorphic adenoma of the salivary glands] PubMed
Adenomatous Polyposis ColiDecrease in nerve fibre density in human sigmoid colon circular muscle occurs with growth but not aging. PubMed
Adenomatous Polyposis ColiDextran sulfate sodium-induced colitis-associated neoplasia: a promising model for the development of chemopreventive interventions. PubMed
Adenomatous Polyposis ColiInducible heat shock protein 70 prevents multifocal flat dysplastic lesions and invasive tumors in an inflammatory model of colon cancer. PubMed
Adenomatous Polyposis ColiNew DNA-based biomarkers for oxidative stress and cancer chemoprevention studies. PubMed
Adenomatous PolypsBioassay of captan for possible carcinogenicity. PubMed
AdenomyosisEndothelial nitric oxide synthase in the endometrium during the menstrual cycle in patients with endometriosis and adenomyosis. PubMed
AdenomyosisExpression of endothelial nitric oxide synthase in the uterus of patients with leiomyoma or adenomyosis. PubMed
AdenomyosisGnRH agonist-suppressed expression of nitric oxide synthases and generation of peroxynitrite in adenomyosis. PubMed
AdenomyosisLocalization of endothelial nitric oxide synthase messenger ribonucleic acid by in situ hybridization in ectopic endometrial tissue in patients with adenomyosis. PubMed
Adrenal InsufficiencyReduction of maternal adrenal steroids results in increased VEGF protein without increased eNOS in the ovine placenta. PubMed
AdrenoleukodystrophyPeroxisomal deficiencies are associated with altered activity of endothelial NOS in human fibroblasts. PubMed
Aggressive PeriodontitisImmunolocalization of inducible nitric oxide synthase in localized juvenile periodontitis patients. PubMed
AIDS Dementia ComplexNitric oxide synthase expression and apoptotic cell death in brains of AIDS and AIDS dementia patients. PubMed
AIDS Dementia ComplexTranslational control of inducible nitric oxide synthase expression by arginine can explain the arginine paradox. PubMed
AIDS-Related ComplexA clearer distinction between HIV-1 paired isolates from peripheral blood mononuclear cells of asymptomatic carriers with and without CD8+ T-cells at nef rather than env V3 loci. PubMed
Airway ObstructionCombined neuronal and inducible nitric oxide synthase inhibition in ovine acute lung injury. PubMed
Airway ObstructionIncreased vascular expression of iNOS at day but not at night in asthmatic subjects with increased nocturnal airway obstruction. PubMed
Airway ObstructionNeuronal nitric oxide synthase is associated with airway obstruction in BALB/c mice exposed to ozone. PubMed
Airway ObstructionNitric oxide synthases and protein oxidation in the quadriceps femoris of patients with chronic obstructive pulmonary disease. PubMed
Airway ObstructionRole of nitric oxide in the development and partial reversal of allergen-induced airway hyperreactivity in conscious, unrestrained guinea-pigs. PubMed
AlbuminuriaAminoguanidine induces haematuria of non-glomerular origin in spontaneously hypertensive rats. PubMed
AlbuminuriaAnti-CD8 monoclonal antibody therapy is effective in the prevention and treatment of experimental autoimmune glomerulonephritis. PubMed
AlbuminuriaAssociation of the Glu298Asp polymorphism of the eNOS Gene with ischemic heart disease in Japanese diabetic subjects. PubMed
AlbuminuriaChronic inhibition of nitric oxide synthase in Heymann nephritis. PubMed
AlbuminuriaL-arginine supplementation accelerates renal fibrosis and shortens life span in experimental lupus nephritis. PubMed
AlbuminuriaT-786C polymorphism of the endothelial nitric oxide synthase gene is associated with albuminuria in the diabetes heart study. PubMed
AlkalosisAlkalosis stimulates endothelial nitric oxide synthase in cultured human pulmonary arterial endothelial cells. PubMed
Alopecia AreataSusceptible and protective endothelial nitric oxide synthase gene polymorphism in alopecia areata in the Kuwaiti population. PubMed
Alveolar Bone LossInducible nitric oxide synthase mediates bone development and P. gingivalis-induced alveolar bone loss. PubMed
Alveolar Bone LossNitric oxide synthase inhibition prevents alveolar bone resorption in experimental periodontitis in rats. PubMed
Alveolar Bone LossOxidative stress causes alveolar bone loss in metabolic syndrome model mice with type 2 diabetes. PubMed
Alveolitis, Extrinsic AllergicInducible nitric oxide synthase, but not xanthine oxidase, is highly expressed in interstitial pneumonias and granulomatous diseases of human lung. PubMed
Alzheimer DiseaseAberrant expression of NOS isoforms in Alzheimer's disease is structurally related to nitrotyrosine formation. PubMed
Alzheimer DiseaseAberrant expression of the constitutive endothelial nitric oxide synthase gene in Alzheimer disease. PubMed
Alzheimer DiseaseAdvanced glycation endproducts co-localize with inducible nitric oxide synthase in Alzheimer's disease. PubMed
Alzheimer DiseaseAngiotensin converting enzyme and endothelial nitric oxide synthase DNA polymorphisms and late onset Alzheimer's disease. PubMed
Alzheimer DiseaseAssociation of a functional NOS1 promoter repeat with Alzheimer's disease in the VITA cohort. PubMed
Alzheimer DiseaseAssociation of neuronal nitric oxide synthase C276T polymorphism with Alzheimer's disease. PubMed
Alzheimer DiseaseBasal forebrain nitric oxide synthase (NOS)-containing neurons project to microvessels and NOS neurons in the rat neocortex: cellular basis for cortical blood flow regulation. PubMed
Alzheimer DiseaseBerberine chloride can ameliorate the spatial memory impairment and increase the expression of interleukin-1beta and inducible nitric oxide synthase in the rat model of Alzheimer's disease. PubMed
Alzheimer DiseaseBeta-amyloid inhibits NOS activity by subtracting NADPH availability. PubMed
Alzheimer DiseaseCerebral Blood Flow Regulation by Nitric Oxide in Alzheimer's Disease. PubMed
Alzheimer DiseaseCerebral blood flow regulation by nitric oxide in neurological disorders. PubMed
Alzheimer DiseaseCharacterization and regional distribution of nitric oxide synthase in the human brain during normal ageing. PubMed
Alzheimer DiseaseClinical experience with risperidone, haloperidol, and thioridazine for dementia-associated behavioral disturbances. PubMed
Alzheimer DiseaseExpression of inducible nitric oxide synthase in the brains of scrapie-infected mice. PubMed
Alzheimer DiseaseGenetic association between endothelial nitric oxide synthase and Alzheimer disease. PubMed
Alzheimer DiseaseImmunohistochemical study of the expression of cytokines and nitric oxide synthases in brains of patients with dementia with Lewy bodies. PubMed
Alzheimer DiseaseInducible nitric oxide synthase immunoreactivity in the Alzheimer disease hippocampus: association with Hirano bodies, neurofibrillary tangles, and senile plaques. PubMed
Alzheimer DiseaseInducible nitric oxide synthase in tangle-bearing neurons of patients with Alzheimer's disease. PubMed
Alzheimer DiseaseInduction of cyclooxygenase-2 and peroxisome proliferator-activated receptor-gamma during nitric oxide-induced apoptotic PC12 cell death. PubMed
Alzheimer DiseaseInflammatory mediator and beta-amyloid (25-35)-induced ceramide generation and iNOS expression are inhibited by vitamin E. PubMed
Alzheimer DiseaseInhibition of interleukin-1-induced uveitis and corneal fibroblast proliferation by interleukin-1 blockers. PubMed
Alzheimer DiseaseMetallothionein-3 and neuronal nitric oxide synthase levels in brains from the Tg2576 mouse model of Alzheimer's disease. PubMed
Alzheimer DiseaseNeuronal and glial coexpression of argininosuccinate synthetase and inducible nitric oxide synthase in Alzheimer disease. PubMed
Alzheimer DiseaseNeuronal nitric oxide synthase (nNOS) mRNA expression and NADPH-diaphorase staining in the frontal cortex, visual cortex and hippocampus of control and Alzheimer's disease brains. PubMed
Alzheimer DiseaseNitration of tyrosine 10 critically enhances amyloid ? aggregation and plaque formation. PubMed
Alzheimer DiseaseNitric oxide synthase gene polymorphisms in Alzheimer's disease and dementia with Lewy bodies. PubMed
Alzheimer DiseasenNOS expressing neurons in the entorhinal cortex and hippocampus are affected in patients with Alzheimer's disease. PubMed
Alzheimer DiseaseNo association between Glu298Asp endothelial nitric oxide synthase polymorphism and Italian sporadic Alzheimer's disease. PubMed
Alzheimer DiseaseNo association between the neuronal nitric oxide synthase gene polymorphism and Alzheimer Disease. PubMed
Alzheimer DiseaseNo evidence for an association between the Glu298Asp polymorphism of the NOS3 gene and Alzheimer's disease. PubMed
Alzheimer DiseaseNO synthase 2 (NOS2) deletion promotes multiple pathologies in a mouse model of Alzheimer's disease. PubMed
Alzheimer DiseaseProcessing of amyloid precursor protein as a biochemical link between atherosclerosis and Alzheimer's disease. PubMed
Alzheimer DiseaseProtection from Alzheimer's-like disease in the mouse by genetic ablation of inducible nitric oxide synthase. PubMed
Alzheimer DiseaseRedox metals and oxidative abnormalities in human prion diseases. PubMed
Alzheimer DiseaseReduced expression of NO-sensitive guanylyl cyclase in reactive astrocytes of Alzheimer disease, Creutzfeldt-Jakob disease, and multiple sclerosis brains. PubMed
Alzheimer DiseaseReduction in asymmetrical dimethylarginine, an endogenous nitric oxide synthase inhibitor, in the cerebrospinal fluid during aging and in patients with Alzheimer's disease. PubMed
Alzheimer DiseaseRelative sparing of nitric oxide synthase-containing neurons in the hippocampal formation in Alzheimer's disease. PubMed
Alzheimer DiseaseSpectrofluorimetric analysis of the interaction of amyloid peptides with neuronal nitric oxide synthase: implications in Alzheimer's disease. PubMed
Alzheimer DiseaseTumor necrosis factor-alpha and nerve growth factor synergistically induce iNOS in pheochromocytoma cells. PubMed
Alzheimer Disease[Reduced neuronal nitric oxide synthetase and c-protein kinase levels in Alzheimer's disease] PubMed
Amblyopia[Histochemical studies on the change of nitric oxide synthetase in lateral geniculate nucleus of monocular deprived kittens] PubMed
AmeloblastomaA survey of epithelial odontogenic tumors and cysts in dogs and cats. PubMed
AmeloblastomaExpression of inducible nitric oxide synthase and vascular endothelial growth factor in ameloblastoma. PubMed
AmeloblastomaImmunohistochemical analysis of inducible nitric oxide synthase (iNOS) and heat shock proteins (HSPs) in ameloblastomas. PubMed
AmnesiaInhibitors of PLA2 and NO synthase cooperate in producing amnesia of a spatial task. PubMed
Amnesia, RetrogradeRole of hippocampal NO in the acquisition and consolidation of inhibitory avoidance learning. PubMed
Amyotrophic Lateral SclerosisColocalization of NOS and SOD1 in neurofilament accumulation within motor neurons of amyotrophic lateral sclerosis: an immunohistochemical study. PubMed
Amyotrophic Lateral SclerosisExpression of nitric oxide synthase isoforms in spinal cord in amyotrophic lateral sclerosis. PubMed
Amyotrophic Lateral SclerosisGlial cells of the spinal cord and subcortical white matter up-regulate neuronal nitric oxide synthase in sporadic amyotrophic lateral sclerosis. PubMed
Amyotrophic Lateral SclerosisIncreased expression of neuronal nitric oxide synthase spliced variants in reactive astrocytes of amyotrophic lateral sclerosis human spinal cord. PubMed
Amyotrophic Lateral SclerosisInducible nitric oxide synthase up-regulation in a transgenic mouse model of familial amyotrophic lateral sclerosis. PubMed
Amyotrophic Lateral SclerosisiNOS and nitrotyrosine immunoreactivity in amyotrophic lateral sclerosis. PubMed
Amyotrophic Lateral SclerosisLack of involvement of neuronal nitric oxide synthase in the pathogenesis of a transgenic mouse model of familial amyotrophic lateral sclerosis. PubMed
Amyotrophic Lateral SclerosisMechanism of selective motor neuronal death after exposure of spinal cord to glutamate: involvement of glutamate-induced nitric oxide in motor neuron toxicity and nonmotor neuron protection. PubMed
Amyotrophic Lateral SclerosisMotor neuron degeneration in amyotrophic lateral sclerosis mutant superoxide dismutase-1 transgenic mice: mechanisms of mitochondriopathy and cell death. PubMed
Amyotrophic Lateral SclerosisNeuritic sprouting with aberrant expression of the nitric oxide synthase III gene in neurodegenerative diseases. PubMed
Amyotrophic Lateral SclerosisNeuronal nitric oxide synthase immunoreactivity in the spinal cord in amyotrophic lateral sclerosis. PubMed
Amyotrophic Lateral SclerosisNeuronal NOS is dislocated during muscle atrophy in amyotrophic lateral sclerosis. PubMed
Amyotrophic Lateral SclerosisNO orchestrates the loss of synaptic boutons from adult 'sick' motoneurons: modeling a molecular mechanism. PubMed
Amyotrophic Lateral SclerosisSequestration of nNOS in neurofilamentous aggregate bearing neurons in vitro leads to enhanced NMDA-mediated calcium influx. PubMed
AnaphylaxisAn inhibitor of nitric oxide synthase, N omega-nitro-L-arginine-methyl ester, attenuates hypotension but does not improve cardiac depression in anaphylaxis in dogs. PubMed
AnaphylaxisAnaphylactic hepatic venoconstriction is attenuated by nitric oxide released via shear stress-dependent and -independent mechanisms in Guinea pig. PubMed
AnaphylaxisAnaphylactic shock depends on PI3K and eNOS-derived NO. PubMed
AnaphylaxisAnaphylaxis: lessons from mouse models. PubMed
AnaphylaxisEffects of hyperthermia pretreatment on expression of heme oxygenase-1 and nitric oxide synthase in rats subjected to experimental anaphylactic shock. PubMed
AnaphylaxisExpression of inducible nitric oxide synthase in a mouse model of anaphylaxis. PubMed
AnaphylaxisNitric oxide in shock. PubMed
AnaphylaxisParticipation of nitric oxide in mouse anaphylactic hypotension. PubMed
AnaphylaxisParticipation of nitric oxide in the sympathetic response to anaphylactic hypotension in anesthetized dogs. PubMed
AnaplasiaIntraobserver reproducibility in assigning brain tumors to classes in the World Health Organization diagnostic scheme. The Childhood Brain Tumor Consortium. PubMed
AnaplasmosisCommentary: adaptive immunity in the absence of innate immune responses? The un-Tolled truth of the silent invaders. PubMed
AnemiaA potential role for inducible nitric oxide synthase in the cerebral response to acute hemodilution. PubMed
AnemiaAnemia and cerebral outcomes: many questions, fewer answers. PubMed
AnemiaHematologic features of iron deficiency anemia in llamas. PubMed
AnemiaHemodilutional anemia is associated with increased cerebral neuronal nitric oxide synthase gene expression. PubMed
AnemiaIncreased cerebral and renal endothelial nitric oxide synthase gene expression after cardiopulmonary bypass in the rat. PubMed
AnemiaNitric oxide and peroxynitrite production by human erythrocytes: a causative factor of toxic anemia in breast cancer patients. PubMed
AnemiaPriming of hypoxia-inducible factor by neuronal nitric oxide synthase is essential for adaptive responses to severe anemia. PubMed
Anemia, AplasticIncreased inducible nitric oxide synthase expression and nitric oxide concentration in patients with aplastic anemia. PubMed
Anemia, HypochromicDevelopment of necrosis and activation of disease resistance in transgenic tobacco plants with severely reduced catalase levels. PubMed
Anemia, Iron-DeficiencyHematologic features of iron deficiency anemia in llamas. PubMed
Anemia, Iron-DeficiencyUp-regulation of renal and vascular nitric oxide synthase in iron-deficiency anemia. PubMed
Anemia, MegaloblasticRefractory Pancytopenia and Megaloblastic Anemia due to Falciparum Malaria. PubMed
Anemia, Refractory, with Excess of BlastsCD117/CD34 expression in leukemic blasts. PubMed
Anemia, Sickle CellAbnormal modulation of cell protective systems in response to ischemic/reperfusion injury is important in the development of mouse sickle cell hepatopathy. PubMed
Anemia, Sickle CellDivergent nitric oxide bioavailability in men and women with sickle cell disease. PubMed
Anemia, Sickle CellHydroxyurea and arginine therapy: impact on nitric oxide production in sickle cell disease. PubMed
Anemia, Sickle CellLow exhaled nitric oxide and a polymorphism in the NOS I gene is associated with acute chest syndrome. PubMed
Anemia, Sickle CellNitric oxide-dependent generation of reactive species in sickle cell disease. Actin tyrosine induces defective cytoskeletal polymerization. PubMed
Anemia, Sickle CellPost-translational inactivation of endothelial nitric oxide synthase in the transgenic sickle cell mouse penis. PubMed
AneurysmApelin signaling antagonizes Ang II effects in mouse models of atherosclerosis. PubMed
AneurysmCoronary artery aneurysm induced by Kawasaki disease in children show features typical senescence. PubMed
AneurysmDisruption of gene for inducible nitric oxide synthase reduces progression of cerebral aneurysms. PubMed
AneurysmEndothelial nitric oxide synthase gene single-nucleotide polymorphism predicts cerebral vasospasm after aneurysmal subarachnoid hemorrhage. PubMed
AneurysmEndothelial nitric oxide synthase stimulates aneurysm growth in aged mice. PubMed
AneurysmErythropoietin increases circulating endothelial progenitor cells and reduces the formation and progression of cerebral aneurysm in rats. PubMed
AneurysmHistological and genetic studies in patients with bicuspid aortic valve and ascending aorta complications. PubMed
AneurysmInfluence of endothelial nitric oxide synthase T-786C single nucleotide polymorphism on aneurysm size. PubMed
AneurysmInhibition of inducible nitric oxide synthase limits nitric oxide production and experimental aneurysm expansion. PubMed
AneurysmMechanisms of occlusion and recanalization in canine carotid bifurcation aneurysms embolized with platinum coils: an alternative concept. PubMed
AneurysmOngoing angiogenesis in blood vessels of the abdominal aortic aneurysm. PubMed
AneurysmPolymorphisms in the endothelial nitric oxide synthase gene and the tendency for aneurysm rupture. PubMed
AneurysmPrevention of rat cerebral aneurysm formation by inhibition of nitric oxide synthase. PubMed
AneurysmThe presence of tandem endothelial nitric oxide synthase gene polymorphisms identifying brain aneurysms more prone to rupture. PubMed
AneurysmUpdate on genetic evidence for rupture-prone compared with rupture-resistant intracranial saccular aneurysms. PubMed
Aneurysm, RupturedEndothelial nitric oxide synthase T-786C single nucleotide polymorphism: a putative genetic marker differentiating small versus large ruptured intracranial aneurysms. PubMed
Angina PectorisEndothelial nitric oxide synthase gene mutation and human leukocyte antigen analyzed in three cases of familial vasospastic angina pectoris. PubMed
Angina PectorisEndothelial nitric oxide synthase T-786C mutation, a reversible etiology of Prinzmetal's angina pectoris. PubMed
Angina Pectoris, VariantEndothelial nitric oxide synthase T-786C mutation, a reversible etiology of Prinzmetal's angina pectoris. PubMed
Angina, StableSerum neopterin, nitric oxide, inducible nitric oxide synthase and tumor necrosis factor-alpha levels in patients with ischemic heart disease. PubMed
Angina, UnstableHuman adaptation to ischemia by preconditioning or unstable angina: involvement of nuclear factor kappa B, but not hypoxia-inducible factor 1 alpha in the heart. PubMed
Angina, UnstableInduction of inflammatory mediators during reperfusion of the human heart. PubMed
Angina, UnstablePrior aspirin use in unstable angina patients with modified plasma inflammatory markers and endothelial nitric oxide synthase in neutrophils. PubMed
Angina, UnstableUnstable angina activates myocardial heat shock protein 72, endothelial nitric oxide synthase, and transcription factors NFkappaB and AP-1. PubMed
AnthraxCloning, expression, and characterization of recombinant nitric oxide synthase-like protein from Bacillus anthracis. PubMed
AnthraxThe Heart Is an Early Target of Anthrax Lethal Toxin in Mice: A Protective Role for Neuronal Nitric Oxide Synthase (nNOS). PubMed
AnthraxThe heart is an early target of anthrax lethal toxin in mice: a protective role for neuronal nitric oxide synthase (nNOS). PubMed
Aortic AneurysmEndothelial nitric oxide synthase stimulates aneurysm growth in aged mice. PubMed
Aortic AneurysmLocally different endothelial nitric oxide synthase protein levels in ascending aortic aneurysms of bicuspid and tricuspid aortic valve. PubMed
Aortic AneurysmSuppression of experimental aortic aneurysms: comparison of inducible nitric oxide synthase and cyclooxygenase inhibitors. PubMed
Aortic Aneurysm, AbdominalAllele frequency of human endothelial nitric oxide synthase gene polymorphism in abdominal aortic aneurysm. PubMed
Aortic Aneurysm, AbdominalExperimental abdominal aortic aneurysms in mice lacking expression of inducible nitric oxide synthase. PubMed
Aortic Aneurysm, AbdominalInducible nitric oxide synthase is present in human abdominal aortic aneurysm and promotes oxidative vascular injury. PubMed
Aortic Aneurysm, AbdominalInhibition of inducible nitric oxide synthase limits nitric oxide production and experimental aneurysm expansion. PubMed
Aortic Aneurysm, AbdominalNeovascularization in the abdominal aortic aneurysm. Endothelial nitric oxide synthase, nitric oxide, and elastolysis. PubMed
Aortic Aneurysm, AbdominalRelationship between iNOS expression and aortic cell proliferation and apoptosis in an elastase-induced model of aorta aneurysm and the effect of 1400 W administration. PubMed
Aortic Aneurysm, AbdominalRole of uncoupled endothelial nitric oxide synthase in abdominal aortic aneurysm formation: treatment with folic acid. PubMed
Aortic Aneurysm, AbdominalSelective inducible nitric oxide synthase (iNOS) inhibition attenuates remote acute lung injury in a model of ruptured abdominal aortic aneurysm. PubMed
Aortic Aneurysm, AbdominalSuppression of experimental aortic aneurysms: comparison of inducible nitric oxide synthase and cyclooxygenase inhibitors. PubMed
Aortic Aneurysm, Abdominal[Role of nitric oxide and inducible nitric oxide synthase in human abdominal aortic aneurysms: a preliminary study.] PubMed
Aortic CoarctationEnhanced nitric oxide inactivation in aortic coarctation-induced hypertension. PubMed
Aortic CoarctationRole of nitric oxide and prostanoids in attenuation of rapid baroreceptor resetting. PubMed
Aortic CoarctationRole of nitric oxide in ginsenoside Rg(1)-induced protection against left ventricular hypertrophy produced by abdominal aorta coarctation in rats. PubMed
Aortic RuptureCardiovascular lesions in bovine Marfan syndrome. PubMed
Aortic Valve StenosisEffects of aortic stenosis on renal renin, angiotensin receptor, endothelin and NOS gene expression in rats. PubMed
ApneaNeurological status, sleep apnea frequency and blood oxygenation in six weeks old infants. PubMed
AppendicitisNitric oxide synthetase and Helicobacter pylori in patients undergoing appendicectomy. PubMed,  PubMed
Argininosuccinic AciduriaEvaluation of endogenous nitric oxide synthesis in congenital urea cycle enzyme defects. PubMed
Arrhythmias, CardiacCardiac dys-synchronization and arrhythmia in hyperhomocysteinemia. PubMed
Arrhythmias, CardiacErythropoietin protects the heart from ventricular arrhythmia during ischemia and reperfusion via neuronal nitric oxide synthase. PubMed
Arrhythmias, CardiacNeuronal nitric oxide synthase protects against myocardial infarction-induced ventricular arrhythmia and mortality in mice. PubMed
Arrhythmias, CardiacProtective effect of polydatin against ischemia/reperfusion injury in rat heart. PubMed
Arterial Occlusive DiseasesBiochemical evidence for impaired nitric oxide synthesis in patients with peripheral arterial occlusive disease. PubMed
Arterial Occlusive DiseasesDetermination of asymmetrical dimethylarginine by capillary electrophoresis-laser-induced fluorescence. PubMed
Arterial Occlusive DiseasesMechanisms of ischemia-induced cavernosal smooth muscle relaxation impairment in a rabbit model of vasculogenic erectile dysfunction. PubMed
ArteriosclerosisChronic blockade of NO synthase activity induces a proinflammatory phenotype in the arterial wall: prevention by angiotensin II antagonism. PubMed
ArteriosclerosisDecreased transplant arteriosclerosis in endothelial nitric oxide synthase-deficient mice. PubMed
ArteriosclerosisEndothelial nitric oxide synthase protects aortic allografts from the development of transplant arteriosclerosis. PubMed
ArteriosclerosisExacerbated transplant arteriosclerosis in inducible nitric oxide-deficient mice. PubMed
ArteriosclerosisExpression of endomyocardial nitric oxide synthase and coronary endothelial function in human cardiac allografts. PubMed
ArteriosclerosisIncreased expression of transforming growth factor-beta and eosinophil infiltration is associated with the development of transplant arteriosclerosis in long-term surviving cardiac allografts. PubMed
ArteriosclerosisIndirect allorecognition can play an important role in the development of transplant arteriosclerosis. PubMed
ArteriosclerosisInducible and endothelial nitric oxide synthase expression during development of transplant arteriosclerosis in rat aortic grafts. PubMed
ArteriosclerosisInducible nitric oxide synthase expression in coronary arteries of transplanted human hearts with accelerated graft arteriosclerosis. PubMed
ArteriosclerosisInducible nitric oxide synthase inhibition of weibel-palade body release in cardiac transplant rejection. PubMed
ArteriosclerosisInducible nitric oxide synthase suppresses the development of allograft arteriosclerosis. PubMed
ArteriosclerosisInduction of inducible NO synthase in bystander human T cells increases allogeneic responses in the vasculature. PubMed
ArteriosclerosisLack of association between 27-bp repeat polymorphism in intron 4 of the endothelial nitric oxide synthase gene and the risk of coronary artery disease. PubMed
ArteriosclerosisNitric oxide and endoplasmic reticulum stress. PubMed
ArteriosclerosisNovel anti-inflammatory actions of amlodipine in a rat model of arteriosclerosis induced by long-term inhibition of nitric oxide synthesis. PubMed
ArteriosclerosisProtective genes in the vessel wall: Modulators of graft survival and function. PubMed
ArteriosclerosisRole of inducible nitric oxide synthase in transplant arteriosclerosis. PubMed
ArteriosclerosisT cell-mediated vascular dysfunction of human allografts results from IFN-gamma dysregulation of NO synthase. PubMed
ArteriosclerosisUpregulation and modulation of inducible nitric oxide synthase in rat cardiac allografts with chronic rejection and transplant arteriosclerosis. PubMed
Arteriosclerosis[Effects of recombinant adenovirus encoding human apM1 gene on proliferation and nitric oxide synthase activity in human umbilical vein endothelial cells] PubMed
Arteriovenous FistulaCharacterization of a model of an arteriovenous fistula in the rat: the effect of L-NAME. PubMed
Arteriovenous FistulaFlow-induced arterial enlargement is inhibited by suppression of nitric oxide synthase activity in vivo. PubMed
ArteritisAssociation of a functional inducible nitric oxide synthase promoter variant with susceptibility to biopsy-proven giant cell arteritis. PubMed
ArteritisEndothelial nitric oxide synthase gene polymorphisms in giant cell arteritis. PubMed
ArteritisEndothelial nitric oxide synthase haplotype associations in biopsy-proven giant cell arteritis. PubMed
ArteritisGiant cell and Takayasu arteritis. PubMed
ArthralgiaA quantitative approach to measure joint pain in experimental osteoarthritis--evidence of a role for nitric oxide. PubMed
ArthritisAggravation of cold-restraint stress-induced gastric lesions in adjuvant arthritic rats: pathogenic role of inducible and endothelial nitric oxide. PubMed
ArthritisAnchored plasticity opens doors for selective inhibitor design in nitric oxide synthase. PubMed
ArthritisAnti-inflammatory effect of all-trans-retinoic acid in inflammatory arthritis. PubMed
ArthritisAnti-inflammatory effect of recombinant human superoxide dismutase in rats and mice and its mechanism. PubMed
ArthritisArginase levels are increased in patients with rheumatoid arthritis. PubMed
ArthritisChanges in nitric oxide synthase isoforms in the spinal cord of rat following induction of chronic arthritis. PubMed
ArthritisChronic restraint stress aggravated arthritic joint swell of rats through regulating nitric oxide production. PubMed
ArthritisComparison of the nitric oxide synthase inhibitors methylarginine and aminoguanidine as prophylactic and therapeutic agents in rat adjuvant arthritis. PubMed
ArthritisContractile, but not endothelial, dysfunction in early inflammatory arthritis: a possible role for matrix metalloproteinase-9. PubMed
ArthritisDifferential function of nitric oxide in murine antigen-induced arthritis. PubMed
ArthritisEffects of nitric oxide on neutrophil influx depends on the tissue: role of leukotriene B4 and adhesion molecules. PubMed
ArthritisElevated serum nitric oxide levels in patients with inflammatory arthritis associated with co-expression of