Information on EC 1.14.11.3 - pyrimidine-deoxynucleoside 2'-dioxygenase:

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The expected taxonomic range for this enzyme is: Dikarya


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EC NUMBERCOMMENTARY
1.14.11.3-

RECOMMENDED NAMEGeneOntology No.
pyrimidine-deoxynucleoside 2'-dioxygenaseGO:0047080

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
2'-deoxyuridine + 2-oxoglutarate + O2 = uridine + succinate + CO2
show the reaction diagram
----

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
oxidation----
oxidative decarboxylation----
redox reaction----
reduction----

PATHWAYKEGG LinkMetaCyc Link
No entries in this field

SYSTEMATIC NAMEIUBMB Comments
2'-deoxyuridine,2-oxoglutarate:oxygen oxidoreductase (2'-hydroxylating)Requires Fe(II) and ascorbate. Also acts on thymidine. cf. EC 1.14.11.10, pyrimidine-deoxynucleoside 1'-dioxygenase.

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
deoxyuridine 2'-dioxygenase----
deoxyuridine 2'-hydroxylase----
pyrimidine deoxyribonucleoside 2'-hydroxylase----
thymidine 2'-dioxygenase----
thymidine 2'-hydroxylase----
thymidine 2-oxoglutarate dioxygenase----
thymidine dioxygenase----

CAS REGISTRY NUMBERCOMMENTARY
9076-89-5-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Neurospora crassa-439172, 439174, 439175, 439176, 439177, 439177--Manually annotated by BRENDA team
Neurospora crassa 1A-439175, 439176, 439177--Manually annotated by BRENDA team
Neurospora crassa STA-439172, 439174--Manually annotated by BRENDA team
Rhodotorula glutinis-439137, 439141, 439170--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
5-bromodeoxyuridine + 2-oxoglutarate + O25-bromouridine + succinate + CO2
show the reaction diagram
Neurospora crassa, Neurospora crassa STA--439174--?
5-hydroxymethyldeoxyuridine + 2-oxoglutarate + O25-hydroxymethyluridine + succinate + CO2
show the reaction diagram
Neurospora crassa, Neurospora crassa STA--439174--?
thymidine + 2-oxoglutarate + O25-methyluridine + succinate + CO2
show the reaction diagram
Neurospora crassa--439174---
thymidine + 2-oxoglutarate + O25-methyluridine + succinate + CO2
show the reaction diagram
Neurospora crassa, Neurospora crassa 1A-conversion to the ribonucleotide prior to its utilization for DNA synthesis439176-439176?
thymidylate + 2-oxoglutarate + O25-methyluridine 5'-phosphate + succinate + CO2
show the reaction diagram
Neurospora crassa, Neurospora crassa STA--439174--?
6-azathymidine + 2-oxoglutarate + O25-mehyluridine + succinate + CO2
show the reaction diagram
Neurospora crassa, Neurospora crassa STA--439174--?
additional information?-Neurospora crassa, Neurospora crassa STA-only pyrimidine deoxyribonucleosides with oxygen at carbon 2 and 4 are active439174---

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
No entries in this field

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
No entries in this field

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
Fe2+Rhodotorula glutinis--439141
Fe2+Neurospora crassa--439174, 439175, 439176, 439177

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
1,10-phenanthrolineNeurospora crassa-at 0.02 mM complete inhibition439174 2D-image
2-ketoadipateNeurospora crassa-38% inhibition at 2.5 mM439174 2D-image
2-ketopimelateNeurospora crassa-26% inhibition at 2.5 mM439174 2D-image
3-ketoadipateNeurospora crassa-50% inhibition at 2.5 mM439174 2D-image
3-oxoglutarateNeurospora crassa-27% inhibition at 2.5 mM439174 2D-image
Co2+Neurospora crassa-95% inhibition at 0.25 mM439174 2D-image
Cu2+Neurospora crassa-95% inhibition at 0.25 mM439174 2D-image
DiglycolateNeurospora crassa-18% inhibition at 2.5 mM439174 2D-image
glutamateNeurospora crassa-20% inhibition at 2.5 mM439174 2D-image
iminodiacetateNeurospora crassa-28% inhibition at 2.5 mM439174 2D-image
Mn2+Neurospora crassa-60% inhibition at 0.25 mM439174 2D-image
N2Neurospora crassa-complete inhibition by exclusion of oxygen439176 2D-image
Ni2+Neurospora crassa-95% inhibition at 0.25 mM439174 2D-image
ThiodiglycolateNeurospora crassa-24% inhibition at 2.5 mM439174 2D-image
Zn2+Neurospora crassa-95% inhibition at 0.25 mM439174 2D-image

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
ascorbateRhodotorula glutinis-no enzyme activity detected when omitted from standard incubation mixture439141 2D-image
ascorbateNeurospora crassa-3fold activation at 5 mM439174 2D-image
ascorbateNeurospora crassa--439175, 439176, 439177 2D-image
catalaseRhodotorula glutinis-30% activation at 0.33 mg per ml catalase439141-
catalaseNeurospora crassa-1.5fold activation at 2 mg per ml439174-
catalaseNeurospora crassa--439175-

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.3-2-oxoglutarateNeurospora crassa--439174 2D-image
0.15-5-bromodeoxyuridineNeurospora crassa--439174 2D-image
0.29-5-HydroxymethyldeoxyuridineNeurospora crassa--439174 2D-image
0.29-6-AzathymidineNeurospora crassa--439174 2D-image
0.19-DeoxyuridineNeurospora crassa--439174 2D-image
0.45-Fe2+Neurospora crassa-at pH 7.2 in 50 mM phosphate buffer439174 2D-image
0.09-thymidineNeurospora crassa--439174 2D-image
0.07-thymidylateNeurospora crassa--439174 2D-image

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
0.053-Neurospora crassa-with thymidine as substrate439175
0.35-Rhodotorula glutinis--439170
0.97-Rhodotorula glutinis--439137

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
6.5-Neurospora crassa--439174

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
No entries in this field

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
No entries in this field

PDBSCOPCATHORGANISM
No entries in this field

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
47000-Neurospora crassa-gel filtration439172, 439174
64600-Rhodotorula glutinis-gel filtration439137

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
monomerNeurospora crassa-1 * 46000, SDS-PAGE439172
monomerNeurospora crassa STA-1 * 46000, SDS-PAGE-

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
No entries in this field

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-Neurospora crassa-439172
-Rhodotorula glutinis-439137
partial purificationRhodotorula glutinis-439170

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISM (UNIPROT ACCESSION NO.)LINK TO PUBMEDSOURCE
439137Warn-Cramer, B.J.; Macrander, L.A.; Abbott, M.T.Markedly different ascorbate dependencies of the sequential alpha-ketoglutarate dioxygenase reactions catalyzed by an essentially homogeneous thymine 7-hydroxylase from Rhodotorula glutinisJ. Biol. Chem.25810551-105571983Rhodotorula glutinis PubMed
439141Wondrack, L.M.; Hsu, C.A.; Abbott, M.T.Thymine 7-hydroxylase and pyrimidine desoxyribonucleoside 2'-hydroxylase activities in Rhodotorula glutinisJ. Biol. Chem.2536511-65151978Rhodotorula glutinis PubMed
439170Stubbe, J.Identification of two alpha-ketoglutarate-dependent dioxygenases in extracts of Rhodotorula glutinis catalyzing deoxyuridine hydroxylationJ. Biol. Chem.2609972-99751985Rhodotorula glutinis PubMed
439172Holme, E.Purification of thymidine 2'-hydroxylase from Neurospora crassaActa Chem. Scand.37743-7451983Neurospora crassa, Neurospora crassa STA PubMed
439174Bankel, L.; Lindstedt, G.; Lindstedt, S.Thymidine 2-hydroxylation in Neurospora crassaJ. Biol. Chem.2476128-61341972Neurospora crassa, Neurospora crassa STA PubMed
439175Liu, C.K.; Shaffer, P.M.; Slaughter, R.S.; McCroskey, R.P.; Abbott, M.T.Stoichiometry of the pyrimidine deoxyribonucleoside 2-hydroxylase reaction and of the conversions of 5-hydroxymethyluracil to 5-formyluracil and of the latter to uracil-5-carboxylic acidBiochemistry112172-21761972Neurospora crassa 1A, Neurospora crassa PubMed
439176Shaffer, P.M.; McCroskey, R.P.; Abbott, M.T.Substrate specificity of the hydroxylase reaction in which thymidine is converted to thymine ribonucleosideBiochim. Biophys. Acta258387-3941972Neurospora crassa 1A, Neurospora crassa PubMed
439177Shaffer, P.M.; McCroskey, R.P.; Palmatier, R.D.; Midgett, R.J.; Abbott, M.T.The cell-free conversion of a deoxyribonucleoside to a ribonucleoside without detachment of the deoxyriboseBiochem. Biophys. Res. Commun.33806-8111968Neurospora crassa 1A, Neurospora crassa PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 1.14.11.3)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)