Information on EC 1.1.3.20 - long-chain-alcohol oxidase:
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EC NUMBERCOMMENTARY
1.1.3.20-

RECOMMENDED NAMEGeneOntology No.
long-chain-alcohol oxidaseGO:0046577

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
a long-chain alcohol + O2 = a long-chain aldehyde + H2O2
show the reaction diagram		----
a long-chain alcohol + O2 = a long-chain aldehyde + H2O2
show the reaction diagram		2 long-chain alcohol + O2 = 2 long-chain aldehyde + 2 H2O2Tanacetum vulgare-389731
a long-chain alcohol + O2 = a long-chain aldehyde + H2O2
show the reaction diagram		2 long-chain alcohol + O2 = 2 long-chain aldehyde + 2 H2O2Simmondsia chinensis-389732
a long-chain alcohol + O2 = a long-chain aldehyde + H2O2
show the reaction diagram		2 long-chain alcohol + O2 = 2 long-chain aldehyde + 2 H2O2Candida tropicalis-389734, 389736, 389737
a long-chain alcohol + O2 = a long-chain aldehyde + H2O2
show the reaction diagram		2 long-chain alcohol + O2 = 2 long-chain aldehyde + 2 H2O2Starmerella bombicola-389735
a long-chain alcohol + O2 = a long-chain aldehyde + H2O2
show the reaction diagram		2 long-chain alcohol + O2 = 2 long-chain aldehyde + 2 H2O2Yarrowia lipolytica-389736
a long-chain alcohol + O2 = a long-chain aldehyde + H2O2
show the reaction diagram		2 long-chain alcohol + O2 = 2 long-chain aldehyde + 2 H2O2; ping-pong mechanismCandida tropicalis-389738

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
oxidation----
redox reaction----
reduction----

PATHWAYKEGG LinkMetaCyc Link
No entries in this field

SYSTEMATIC NAMEIUBMB Comments
long-chain-alcohol:oxygen oxidoreductaseOxidizes long-chain fatty alcohols; best substrate is dodecyl alcohol.

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
FAOCandida cloacae, Candida tropicalis--672339
FAOMucor circinelloides--710901
FAOTCandida tropicalis--672339
fatty alcohol oxidase----
fatty alcohol oxidaseAspergillus terreus--671539
fatty alcohol oxidaseMucor circinelloides--710901
fatty alcohol:oxygen oxidoreductase----
LCAOAspergillus terreus--671539
LjFAO1 proteinLotus japonicus--685778
long chain alcohol oxidaseAspergillus terreus--671539
long chain fatty acid oxidaseAspergillus terreus--671539
long chain fatty alcohol oxidaseAspergillus terreus--671539
long-chain fatty acid oxidase----
long-chain fatty alcohol oxidaseCandida cloacae, Candida tropicalis--672339
oxidase, long-chain fatty alcohol----

CAS REGISTRY NUMBERCOMMENTARY
129430-50-8-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Arabidopsis thaliana-655590--Manually annotated by BRENDA team
Aspergillus terreusstrain MTCC 6324, isolated from the oil-contaminated soil sample, collected from an oil field of Assam, enzyme form H3671539--Manually annotated by BRENDA team
Aspergillus terreus MTCC 6324strain MTCC 6324, isolated from the oil-contaminated soil sample, collected from an oil field of Assam, enzyme form H3671539--Manually annotated by BRENDA team
Candida cloacaemultiple isoforms672339--Manually annotated by BRENDA team
Candida cloacaestrain 3152389739--Manually annotated by BRENDA team
Candida cloacae 3152strain 3152389739--Manually annotated by BRENDA team
Candida tropicalis-389734, 389736, 389737, 389738--Manually annotated by BRENDA team
Candida tropicalismultiple isoforms672339--Manually annotated by BRENDA team
Lotus japonicusecotype Gifu B-129685778--Manually annotated by BRENDA team
Mucor circinelloides-710901--Manually annotated by BRENDA team
Simmondsia chinensis-94404--Manually annotated by BRENDA team
Simmondsia chinensisjojoba389732--Manually annotated by BRENDA team
Starmerella bombicolasyn. Torulopsis bombicola389735--Manually annotated by BRENDA team
Tanacetum vulgare-389731--Manually annotated by BRENDA team
Yarrowia lipolytica-389736--Manually annotated by BRENDA team
Yarrowia lipolyticastrain H-222389740--Manually annotated by BRENDA team
Yarrowia lipolytica H-222strain H-222389740--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
1,10-decanediol + O2?
show the reaction diagram		Starmerella bombicola--389735--?
1,14-tetradecanediol + O2?
show the reaction diagram		Starmerella bombicola--389735--?
1,16-hexadecanediol + O2?
show the reaction diagram		Arabidopsis thaliana--655590--?
1,16-hexadecanediol + O2?
show the reaction diagram		Lotus japonicus-purified active LjFAO1 protein685778--?
1-docosanol + O2docosanal + H2O2
show the reaction diagram		Simmondsia chinensis-i.e. behenyl alcohol, poor substrate389732--?
1-dodecanol + O2dodecanal + H2O2
show the reaction diagram		Arabidopsis thaliana--655590--?
1-dodecanol + O2dodecanal + H2O2
show the reaction diagram		Lotus japonicus-purified active LjFAO1 protein685778--?
1-eicosanol + O2eicosanal + H2O2
show the reaction diagram		Candida tropicalis--389737--?
1-eicosanol + O2eicosanal + H2O2
show the reaction diagram		Simmondsia chinensis-i.e. arachidyl alcohol, poor substrate389732--?
1-hexadecanol + O2hexadecanal + H2O2
show the reaction diagram		Arabidopsis thaliana--655590--?
1-hexadecanol + O2hexadecanal + H2O2
show the reaction diagram		Lotus japonicus-purified active LjFAO1 protein685778--?
1-tetradecanol + O2tetradecanal + H2O2
show the reaction diagram		Candida tropicalis--389734-389734?
1-tetradecanol + O2tetradecanal + H2O2
show the reaction diagram		Simmondsia chinensis--389732-389732?
1-tetradecanol + O2tetradecanal + H2O2
show the reaction diagram		Simmondsia chinensis-oxidation at 74% the rate of dodecanol oxidation94404--?
1-tetradecanol + O2tetradecanal + H2O2
show the reaction diagram		Candida tropicalis-75% the rate of dodecanol oxidation389738--?
1-tetradecanol + O2tetradecanal + H2O2
show the reaction diagram		Starmerella bombicola-i.e. myristyl alcohol, best substrate389735--?
12-bromododecanol + O212-bromodecanal + H2O2
show the reaction diagram		Candida tropicalis-oxidation at 92% the rate of dodecanol oxidation389737--?
12-bromododecanol + O212-bromodecanal + H2O2
show the reaction diagram		Starmerella bombicola-oxidation at the same rate as dodecanol389735--?
12-hydroxydodecanoic acid + O2?
show the reaction diagram		Candida tropicalis-poor substrate389738--?
16-hydroxyhexadecanoic acid + O2?
show the reaction diagram		Candida tropicalis--389734, 389737--?
2-dodecanol + O2?
show the reaction diagram		Candida tropicalis--389738--?
alpha,omega-alkanediols + O2?
show the reaction diagram		Candida tropicalis-from C10 to C16389738--?
alpha,omega-alkanediols + O2?
show the reaction diagram		Starmerella bombicola-from C10 to C16389735--?
chrysanthemyl alcohol + O2chrysanthenmyl aldehyde + H2O2
show the reaction diagram		Candida tropicalis--389737--?
cis-11-eicosenol + O2cis-11-eicosenal + H2O2
show the reaction diagram		Simmondsia chinensis--94404--?
cis-11-eicosenol + O2cis-11-eicosenal + H2O2
show the reaction diagram		Simmondsia chinensis-higher oxidation rate than saturated analog389732--?
cis-11-eicosenol + O2cis-11-eicosenal + H2O2
show the reaction diagram		Simmondsia chinensis-oxidation at 19% the rate of dodecanol oxidation94404--?
cis-11-eicosenol + O2cis-11-eicosenal + H2O2
show the reaction diagram		Candida tropicalis-oxidation at 1.9% the rate of dodecanol oxidation389737--?
cis-11-hexadecenol + O2cis-11-hexadecenal + H2O2
show the reaction diagram		Candida tropicalis--389734--?
decanol + O2decanal + H2O2
show the reaction diagram		Candida tropicalis--389734--?
decanol + O2decanal + H2O2
show the reaction diagram		Simmondsia chinensis--389732-389732?
decanol + O2decanal + H2O2
show the reaction diagram		Starmerella bombicola-best substrate389735--?
decanol + O2decanal + H2O2
show the reaction diagram		Candida tropicalis-oxidation at 70% the rate of dodecanol oxidation389738--?
decanol + O2decanal + H2O2
show the reaction diagram		Simmondsia chinensis-oxidation at 51% the rate of dodecanol oxidation94404--?
diol + O2?
show the reaction diagram		Tanacetum vulgare-poor substrates389731--?
diol + O2?
show the reaction diagram		Candida tropicalis-e.g. hexadecan-1,16-diol has an oxidation rate at 31% the rate of dodecanol oxidation389737--?
docosenol + O2docosenal + H2O2
show the reaction diagram		Simmondsia chinensis-oxidation at 13% the rate of dodecanol oxidation94404--?
docosenol + O2docosenal + H2O2
show the reaction diagram		Simmondsia chinensis-higher oxidation rate than saturated analog389732--?
dodecanol + O2dodecanal + H2O2
show the reaction diagram		Starmerella bombicola--389735--?
dodecanol + O2dodecanal + H2O2
show the reaction diagram		Candida tropicalis-i.e. lauryl alcohol, preferred substrate389734-389734?
dodecanol + O2dodecanal + H2O2
show the reaction diagram		Candida tropicalis-i.e. lauryl alcohol, preferred substrate389737, 389738--?
dodecanol + O2dodecanal + H2O2
show the reaction diagram		Simmondsia chinensis-i.e. lauryl alcohol, preferred substrate94404--?
dodecanol + O2dodecanal + H2O2
show the reaction diagram		Simmondsia chinensis-i.e. lauryl alcohol, preferred substrate389732-389732?
farnesol + O2farnesal + H2O2
show the reaction diagram		Candida tropicalis--389737--?
geraniol + O23,7-dimethylocta-trans-2,6-dien-1-al + H2O2
show the reaction diagram		Tanacetum vulgare-i.e. 3,7-dimethylocta-trans-2,6-dien-1-ol, better substrate than cis-isomer, the 2-ene-group enhances activity389731--?
hex-trans-2-ene-1-ol + O2hex-trans-2-ene-1-al + H2O2
show the reaction diagram		Tanacetum vulgare-preferred substrate, the 2-ene-group enhances activity389731-389731?
hexadecanol + O2hexadecanal + H2O2
show the reaction diagram		Candida tropicalis--389734-389734?
hexadecanol + O2hexadecanal + H2O2
show the reaction diagram		Simmondsia chinensis--389732-389732?
hexadecanol + O2hexadecanal + H2O2
show the reaction diagram		Starmerella bombicola--389735--?
hexadecanol + O2hexadecanal + H2O2
show the reaction diagram		Candida tropicalis-19% the rate of dodecanol oxidation389738--?
hexadecanol + O2hexadecanal + H2O2
show the reaction diagram		Candida tropicalis-68% the rate of dodecanol oxidation389737--?
hexadecanol + O2hexadecanal + H2O2
show the reaction diagram		Simmondsia chinensis-i.e. palmityl or cetyl alcohol, oxidation at 26% the rate of dodecanol oxidation94404--?
hexanol + O2hexanal + H2O2
show the reaction diagram		Candida tropicalis--389734-389734?
long-chain alcohol + O2?
show the reaction diagram		Lotus japonicus--685778--?
long-chain alcohol + O2?
show the reaction diagram		Candida tropicalis--389737, 389738--?
long-chain alcohol + O2?
show the reaction diagram		Simmondsia chinensis-reaction in wax ester catabolism in the storage wax bodies of jojoba plant during germination94404, 389732--?
long-chain alcohol + O2?
show the reaction diagram		Tanacetum vulgare-substrates are primary alcohols with a chain-length of C6-C10389731--?
long-chain alcohol + O2?
show the reaction diagram		Candida tropicalis-reaction in n-alkane catabolism389734--?
long-chain alcohol + O2?
show the reaction diagram		Yarrowia lipolytica-n-alkan-inducible enzyme389736--?
long-chain alcohol + O2?
show the reaction diagram		Candida tropicalis-n-alkan-inducible enzyme389734, 389736--?
long-chain alcohol + O2?
show the reaction diagram		Simmondsia chinensis-monounsaturated long-chain alcohols389732--?
long-chain alcohol + O2?
show the reaction diagram		Starmerella bombicola-constitutive enzyme, perhaps two enzymes389735--?
n-octanol + O2n-octanal + H2O2
show the reaction diagram		Candida tropicalis--389734-389734?
n-octanol + O2n-octanal + H2O2
show the reaction diagram		Tanacetum vulgare--389731-389731?
n-octanol + O2n-octanal + H2O2
show the reaction diagram		Starmerella bombicola--389735--?
n-octanol + O2n-octanal + H2O2
show the reaction diagram		Candida tropicalis-oxidation at 34% the rate of dodecanol oxidation389738--?
octadecanol + O2octadecanal + H2O2
show the reaction diagram		Candida tropicalis--389734-389734?
octadecanol + O2octadecanal + H2O2
show the reaction diagram		Simmondsia chinensis--389732-389732?
octadecanol + O2octadecanal + H2O2
show the reaction diagram		Candida tropicalis-oxidation at 12% the rate of dodecanol oxidation389738--?
octadecanol + O2octadecanal + H2O2
show the reaction diagram		Simmondsia chinensis-i.e. stearyl alcohol, oxidation at 16% the rate of dodecanol oxidation94404--?
terpenol + O2?
show the reaction diagram		Tanacetum vulgare-poor substrate389731--?
long-chain alcohol + O2long-chain aldehyde + H2O
show the reaction diagram		Arabidopsis thaliana-involved in fatty acid metabolism655590--?
additional information?-Candida tropicalis-weak activity towards fatty aldehydes389738---
additional information?-Starmerella bombicola-no substrate: omega-hydroxy fatty acids389735---
additional information?-Candida tropicalis-no substrate: dodecan-4-ol, dodecan-5-ol389738---
additional information?-Tanacetum vulgare-substrates are primary alcohols with a chain-length of C6-C10, optimal chain-length: C8-C10389731---
additional information?-Candida tropicalis-no substrates: dodecanal, omega-hydroxydodecanoate389734---
additional information?-Starmerella bombicola-perhaps two long-chain alcohol oxidases in Candida bombicola: the two best substrates are decanol and tetradecanol389735---
additional information?-Tanacetum vulgare-no substrates for Tanacetum enzyme: secondary, tertiary alcohols and Tris389731---
additional information?-Simmondsia chinensis-no substrates: ethanol, palmitic acid94404, 389732---
additional information?-Candida tropicalis-no substrate: geraniol, hexadecan-2-ol, dodecan-5-ol, 2-adamantol, 5-phenylpentan-1-ol, 4-cyclohexylbutan-1-ol, 1-adamantane ethanol389737---
additional information?-Candida cloacae-the enzyme is a c-type haemoprotein and plays an important role in long chain fatty acid metabolism672339---
additional information?-Candida tropicalis-the enzyme is a c-type haemoprotein and plays an important role in long chain fatty acid metabolism, different pathways of octadecane, hexadecane, oleic acid and palmitic acid utilization exist in the organism, overview672339---
additional information?-Aspergillus terreus-the fungal enzyme forms H1, H2, H3, and H4 have similar biochemical properties, but show different substrate specificities from short chain alcohol oxidase for H1, aryl alcohol oxidase for H4, and secondary alcohol oxidase for H2, H3 is a long chain alcohol oxidase, overview671539---

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
cis-11-eicosenol + O2cis-11-eicosenal + H2O2
show the reaction diagram		Simmondsia chinensis--94404--
docosenol + O2docosenal + H2O2
show the reaction diagram		Simmondsia chinensis--94404--
long-chain alcohol + O2?
show the reaction diagram		Lotus japonicus--685778--
long-chain alcohol + O2?
show the reaction diagram		Candida tropicalis--389737, 389738--
long-chain alcohol + O2?
show the reaction diagram		Simmondsia chinensis-reaction in wax ester catabolism in the storage wax bodies of jojoba plant during germination94404, 389732--
long-chain alcohol + O2?
show the reaction diagram		Tanacetum vulgare-substrates are primary alcohols with a chain-length of C6-C10389731--
long-chain alcohol + O2?
show the reaction diagram		Candida tropicalis-reaction in n-alkane catabolism389734--
long-chain alcohol + O2?
show the reaction diagram		Yarrowia lipolytica-n-alkan-inducible enzyme389736--
long-chain alcohol + O2?
show the reaction diagram		Candida tropicalis-n-alkan-inducible enzyme389734, 389736--
long-chain alcohol + O2?
show the reaction diagram		Simmondsia chinensis-monounsaturated long-chain alcohols389732--
long-chain alcohol + O2?
show the reaction diagram		Starmerella bombicola-constitutive enzyme, perhaps two enzymes389735--
long-chain alcohol + O2long-chain aldehyde + H2O
show the reaction diagram		Arabidopsis thaliana-involved in fatty acid metabolism655590--
additional information?-Candida cloacae-the enzyme is a c-type haemoprotein and plays an important role in long chain fatty acid metabolism672339--
additional information?-Candida tropicalis-the enzyme is a c-type haemoprotein and plays an important role in long chain fatty acid metabolism, different pathways of octadecane, hexadecane, oleic acid and palmitic acid utilization exist in the organism, overview672339--

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
FlavinCandida tropicalis-not FAD, FMN, riboflavin, different absorption and fluorescence emission spectra, flavin is probably covalently bound389738 2D-image
FlavinCandida cloacae, Candida tropicalis-enzyme-bound, dependent on672339 2D-image
additional informationTanacetum vulgare-FAD, FMN; NAD(P)+389731-
additional informationCandida tropicalis-no cofactor: riboflavin, 2,6-dichlorophenolindophenol/phenazine methosulfate, potassium ferricyanide, cytochrome c, nitro blue tetrazolium, tetramethyl-p-phenylenediamine389738-
additional informationAspergillus terreus-no effects upon addition or deletion of FAD, flavin mononucleotide, NADP+, and NAD+ in the reaction mixture671539-
additional informationSimmondsia chinensis-NAD(P)+94404-

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
No entries in this field

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
lightCandida tropicalis, Yarrowia lipolytica-enzyme from Yarrowia more sensitive to light than Candida tropicalis, inactivation diminished under anaerobic conditions, light of wavelength longer than 480 nm and shorter than 430 nm does not inactivate; photochemical inactivation at wavelength of 450 nm, i.e. blue-light region of the spectrum of visible light389736-
O2Candida tropicalis, Yarrowia lipolytica-together with light of 405 nm photochemical inactivation389736 2D-image
p-chloromercuribenzoateSimmondsia chinensis-70% loss of activity at 0.1 mM, completely reversible with dithiothreitol389732 2D-image
lightCandida tropicalis-complete inactivation, t1/2: 7 min; photochemical inactivation at wavelength of 450 nm, i.e. blue-light region of the spectrum of visible light389738-
additional informationTanacetum vulgare-no inhibitor: cyanide; no inhibitors: iodoacetate, MnSO4, EDTA, p-hydroxymercuribenzoate, 2,2'-dipyridyl, 1,10-phenanthroline, 8-hydroxyquinoline, fluoride, azide389731-
additional informationSimmondsia chinensis-no inhibitor: cyanide389732, 94404-
additional informationCandida tropicalis-no inhibitors: pyrazole, decanethiol, loss of enzyme activity under anaerobic conditions is reversible by introduction of oxygen389734-
additional informationAspergillus terreus-no effects upon addition or deletion of FAD, flavin mononucleotide, NADP+, and NAD+ in the reaction mixture671539-

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
DetergentsCandida tropicalis-e.g. cholate, activation, purified preparations do not need detergents for activation389738 2D-image
additional informationAspergillus terreus-no effects upon addition or deletion of FAD, flavin mononucleotide, NADP+, and NAD+ in the reaction mixture671539-

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.019-1,16-hexadecanediolLotus japonicus-1 mM, purified active LjFAO1 protein, compared to substrate preferences with the AtFAO3 gene of Arabidopsis thaliana685778 2D-image
0.059-1-DodecanolLotus japonicus-5 mM, purified active LjFAO1 protein, compared to substrate preferences with the AtFAO3 gene of Arabidopsis thaliana685778 2D-image
0.049-1-hexadecanolLotus japonicus-1 mM, purified active LjFAO1 protein, compared to substrate preferences with the AtFAO3 gene of Arabidopsis thaliana685778 2D-image
0.042-DecanolCandida tropicalis--389738 2D-image
0.004-DodecanolCandida cloacae--389739 2D-image
0.0061-DodecanolCandida tropicalis--389738 2D-image
0.008-DodecanolCandida tropicalis-aqueous phase389737 2D-image
4-DodecanolSimmondsia chinensis--94404 2D-image
40-DodecanolCandida tropicalis-octane/aqueous phase 99/1389737 2D-image
1.56-geraniolTanacetum vulgare--389731 2D-image
0.19-Hex-trans-2-ene-1-olTanacetum vulgare--389731 2D-image
0.005-hexadecanolCandida tropicalis--389738 2D-image
0.49-n-OctanolTanacetum vulgare-value above389731 2D-image
0.061-O2Candida tropicalis--389738 2D-image
1-OctanolCandida tropicalis--389738 2D-image
0.0015-TetradecanolCandida tropicalis--389738 2D-image

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
0.0195-Yarrowia lipolytica--389740
0.034-Candida tropicalis-hexadecanol389734
0.035-Candida tropicalis-16-hydroxy-hexadecanoate389734
0.042-Starmerella bombicola-glucose grown, tetradecanol389735
0.07-Candida tropicalis, Yarrowia lipolytica--389736
0.08-Candida cloacae-dodecanol389739
0.144-Starmerella bombicola-n-hexane grown, tetradecanol389735
0.192-Candida tropicalis-cis-11-hexadecanol389734
0.218-Candida tropicalis-dodecanol389734
0.22-Candida tropicalis-decanol, in aqueous phase389737
2.6-Tanacetum vulgare--389731
30-Candida tropicalis--389738
additional information-Lotus japonicus-LjFAO1 gene identified, genomic structure and mRNA expression, cold stress profiling of LjFAO1 transcripts, overexpression of full-lenght and C-truncated cDNAs, full-length version but not C-truncated part of overexpressed LjFAO1-gene shows long-chain fatty alcohol oxidase activity, substrate specificites of purified protein shown685778

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
5.59Tanacetum vulgare-plateau389731
7.68.8Starmerella bombicola-decanol389735
8.18.3Starmerella bombicola-tetradecanol389735
8.59Candida cloacae--389739
8.5-Aspergillus terreus--671539
9-Simmondsia chinensis-1-dodecanol389732
9-Candida tropicalis-60% activity at pH 7.0389738
9-Simmondsia chinensis--94404
9.3-Yarrowia lipolytica--389740

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
4.810Tanacetum vulgare-about half-maximal activity at pH 4.8 and 10.0389731
6.69.3Simmondsia chinensis-about half-maximal activity at pH 6.6 and 9.3389732

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
1822Tanacetum vulgare--389731
20-Candida tropicalis-higher conversions appear at lower temperatures due to an increased stability of the enzyme389737
30-Starmerella bombicola--389735
30-Aspergillus terreus--671539

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
045Tanacetum vulgare-about 60% of maximal activity at 0°C and about 30% of maximal activity at 45°C389731
1835Starmerella bombicola-about 60% of maximal activity at 18°C and 35°C, decanol389735
2038Starmerella bombicola-about half-maximal activity at 20°C and 38°C, tetradecanol389735

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
6-Mucor circinelloides-the enzyme has two isoelectric point components: 6.0 and 6.3, isoelectric focusing710901
6.3-Mucor circinelloides-the enzyme has two isoelectric point components: 6.0 and 6.3, isoelectric focusing710901

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
culture condition:n-decane-grown cellMucor circinelloides--710901Manually annotated by BRENDA team
culture condition:n-hexadecane-grown cellAspergillus terreus--671539Manually annotated by BRENDA team
seedlingLotus japonicus-8-day-old seedlings, RT-PCR, whole plant expression shown, cold stress treatment of685778Manually annotated by BRENDA team
siliqueLotus japonicus-lowest mRNA expression in685778Manually annotated by BRENDA team
leafTanacetum vulgare--389731Manually annotated by BRENDA team
additional informationAspergillus terreus-evaluation of different carbon sources for growth, overview671539Manually annotated by BRENDA team

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
glyoxysomeSimmondsia chinensis--9514389732Manually annotated by BRENDA team
membraneSimmondsia chinensis--16020389732, 94404Manually annotated by BRENDA team
membraneCandida tropicalis--16020389734, 389736, 389737, 389738Manually annotated by BRENDA team
membraneStarmerella bombicola--16020389735Manually annotated by BRENDA team
membraneYarrowia lipolytica--16020389736Manually annotated by BRENDA team
membraneArabidopsis thaliana--16020655590Manually annotated by BRENDA team
membraneCandida cloacae, Candida tropicalis-bound16020672339Manually annotated by BRENDA team
membraneMucor circinelloides-located on the inner face of the membrane16020710901Manually annotated by BRENDA team
microsomeCandida tropicalis---389734, 389736, 389738Manually annotated by BRENDA team
microsomeYarrowia lipolytica---389736Manually annotated by BRENDA team
microsomeCandida cloacae---389739Manually annotated by BRENDA team
microsomeAspergillus terreus---671539Manually annotated by BRENDA team
oil bodySimmondsia chinensis---389732, 94404Manually annotated by BRENDA team
solubleMucor circinelloides---710901Manually annotated by BRENDA team

PDBSCOPCATHORGANISM
No entries in this field

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
73000-Candida cloacae-SDS-PAGE389739
73000-Arabidopsis thaliana-recombinant protein, SDS-PAGE655590
77300-Candida cloacae-calculated from amino acid sequence389739
145000-Candida tropicalis-gel filtration389738
180000-Tanacetum vulgare-gel filtration389731
272000-Aspergillus terreus-isozymes H2, H3, and H4, native PAGE671539
350000-Mucor circinelloides-native enzyme, gel filtration710901

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
dimerTanacetum vulgare-1 * 94000 + 1 * 75000, SDS-PAGE389731
dimerCandida tropicalis-2 * 70000, SDS-PAGE389738
homooctamerMucor circinelloides-8 * 46000, SDS-PAGE710901

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
6-Candida tropicalis-inactivation after 3 min389738
7-Candida tropicalis-and below unstable389738
9-Candida tropicalis-at least 15 min stable389738

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
25-Starmerella bombicola-at least 15 min stable389735
25-Candida tropicalis-inactivation overnight389738
30-Starmerella bombicola-rapid loss of activity389735
30-Aspergillus terreus-unstable above671539
37-Starmerella bombicola-inactivation after 1 min389735
100-Simmondsia chinensis-boiling for 30 min inactivates the enzyme389732

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
CHAPS, 0.5%, stabilizes during solubilizationCandida tropicalis-389738
cholate, 1%, solubilizes and stabilizesCandida tropicalis-389738
lyophilization, membrane preparation stable toCandida tropicalis-389737
phenylmethylsulfonyl fluoride stabilizes during purification and storageCandida tropicalis-389738
prolonged dialysis against EDTA, stable toTanacetum vulgare-389731

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
AcetoneTanacetum vulgare-stable to389731
octaneCandida tropicalis-enzyme stable in 99% octane/1% H2O389737

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
oxygen together with light of 450 nm inactivatesCandida tropicalis, Yarrowia lipolytica-389736

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-70°C, phosphate buffered saline, 25 mM Tris-HCl, pH 7.5, 0.3 M NaCl, 1 mM dithiothreitol, 20% glycerolArabidopsis thaliana-655590
0°C, in the dark stableCandida tropicalis-389736
0°C, in the dark stable for at least 10 hCandida tropicalis-389738
freezing overnight leads to 50% loss of activitySimmondsia chinensis-94404, 389732
0°C, in the dark stableYarrowia lipolytica-389736

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
recombinant protein using His-tagArabidopsis thaliana-655590
homogeneityCandida cloacae-389739
-Candida tropicalis-389738
partialCandida tropicalis-389736
full length version of LjFAO1 protein, gel purificationLotus japonicus-685778
-Mucor circinelloides-710901
partialSimmondsia chinensis-94404
homogeneityTanacetum vulgare-389731
-Yarrowia lipolytica-389740
partialYarrowia lipolytica-389736

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
expressed as a His-tagged protein in Escherichia coliArabidopsis thaliana-655590
-Candida cloacae-389739
full length cDNA and C-terminal deleted protein overexpressed in Escherichia coli BL21(DE3), pET-28a and pGEM-T easy plasmidsLotus japonicus-685778

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
additional informationCandida tropicalis-construction of a faot double knockout mutant, which shows no enzyme activity and is unable to grow on octadecane, but grows on oleic acid and hexadecane, overview672339

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISMLINK TO PUBMEDSOURCE
94404Moreau, R.A.; Huang, A.H.C.Enzymes of wax ester catabolism in jojobaMethods Enzymol.71804-8131981Simmondsia chinensis-
389731Banthorpe, D.V.; Cardemil, E.; Del Carmen Contraras, M.Purification and properties of alcohole oxidase from Tanacetum vulgarePhytochemistry15391-3941976Tanacetum vulgare-
389732Moreau, R.A.; Huang, A.H.C.Oxidation of fatty alcohol in the cotyledons of jojoba seedlingsArch. Biochem. Biophys.194422-4301979Simmondsia chinensis PubMed
389734Kemp, G.D.; Dickinson, F.M.; Ratledge, C.Inducible long chain alcohol oxidase from alkane-grown Candida tropicalisAppl. Microbiol. Biotechnol.29370-3741988Candida tropicalis-
389735Hommel, R.; Ratledge, C.Evidence frr two fatty alcohol oxidases in the biosurfactant-producing yeast Candida (Torulopsis) bombicolaFEMS Microbiol. Lett.70183-1861990Starmerella bombicola PubMed
389736Kemp, G.D.; Dickinson, F.M.; Ratledge, C.Light sensitivity of the n-alkane-induced fatty alcohol oxidase from Candida tropicalis and Yarrowia lipolyticaAppl. Microbiol. Biotechnol.32461-4641990Candida tropicalis, Yarrowia lipolytica-
389737Kemp, G.D.; Dickinson, F.M.; Ratledge, C.Activity and substrate specificity of the fatty alcohol oxidase of Candida tropicalis in organic solventsAppl. Microbiol. Biotechnol.34441-4451991Candida tropicalis-
389738Dickinson, F.M.; Wadforth, C.Purification and some properties of alcohol oxidase from alkane-grown Candida tropicalisBiochem. J.282325-3311992Candida tropicalis PubMed
389739Vanhanen, S.; West, M.; Kroon, J.T.M.; Lindner, N.; Casey, J.; Cheng, Q.; Elborough, K.M.; Slabas, A.R.A consensus sequence for long-chain fatty-acid alcohol oxidases from Candida identifies a family of genes involved in lipid.omega-oxidation in yeast with homologues in plants and bacteriaJ. Biol. Chem.2754445-44522000Candida cloacae PubMed
389740Il'chenko, A.P.; Morgunov, I.G.; Honeck, H.; Mauersberger, S.; Vasilkova, N.N.; Muller, H.G.Purification and some properties of alcohol oxidase from the yeast Yarrowia lipolytica H-222Biokhimiya591312-13191994Yarrowia lipolytica-
655590Cheng, Q.; Liu, H.T.; Bombelli, P.; Smith, A.; Slabas, A.R.Functional identification of AtFao3, a membrane bound long chain alcohol oxidase in Arabidopsis thalianaFEBS Lett.57462-682004Arabidopsis thaliana PubMed
671539Kumar, A.K.; Goswami, P.Functional characterization of alcohol oxidases from Aspergillus terreus MTCC 6324Appl. Microbiol. Biotechnol.72906-9112006Aspergillus terreus PubMed
672339Cheng, Q.; Sanglard, D.; Vanhanen, S.; Liu, H.T.; Bombelli, P.; Smith, A.; Slabas, A.R.Candida yeast long chain fatty alcohol oxidase is a c-type haemoprotein and plays an important role in long chain fatty acid metabolismBiochim. Biophys. Acta1735192-2032005Candida cloacae, Candida tropicalis PubMed
685778Zhao, S.; Lin, Z.; Ma, W.; Luo, D.; Cheng, Q.Cloning and characterization of long-chain fatty alcohol oxidase LjFAO1 in lotus japonicusBiotechnol. Prog.24773-7792008Lotus japonicus PubMed
710901Silva-Jimenez, H.; Zazueta-Novoa, V.; Duron-Castellanos, A.; Rodriguez-Robelo, C.; Leal-Morales, C.A.; Zazueta-Sandoval, R.Intracellular distribution of fatty alcohol oxidase activity in Mucor circinelloides YR-1 isolated from petroleum contaminated soilsAntonie van Leeuwenhoek96527-5352009Mucor circinelloides PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 1.1.3.20)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)