Information on EC 1.1.2.5 - D-lactate dehydrogenase (cytochrome c-553):
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The lowest common taxonomy group for this enzyme is: Desulfovibrio vulgaris

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EC NUMBERCOMMENTARY
1.1.2.5-

RECOMMENDED NAMEGeneOntology No.
D-lactate dehydrogenase (cytochrome c-553)GO:0047051

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
(R)-lactate + 2 ferricytochrome c-553 = pyruvate + 2 ferrocytochrome c-553
show the reaction diagram		----

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
oxidation----
redox reaction----
reduction----

PATHWAYKEGG LinkMetaCyc Link
No entries in this field

SYSTEMATIC NAMEIUBMB Comments
(R)-lactate:ferricytochrome-c-553 2-oxidoreductaseFrom Desulfovibrio vulgaris.

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
D-lactate:ferricytochrome C-553 oxidoreductase----

CAS REGISTRY NUMBERCOMMENTARY
37250-79-6-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Desulfovibrio vulgaris-389630--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
D-lactate + 1-methoxyphenazinium methyl sulfatepyruvate + ?
show the reaction diagram		Desulfovibrio vulgaris--389630--?
D-lactate + 2,6-dichlorophenolindophenolpyruvate + ?
show the reaction diagram		Desulfovibrio vulgaris--389630--?
D-lactate + 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloridepyruvate + ?
show the reaction diagram		Desulfovibrio vulgaris--389630--?
D-lactate + ferricyanidepyruvate + ferrocyanide
show the reaction diagram		Desulfovibrio vulgaris--389630--?
D-lactate + ferricytochrome c-553pyruvate + ferrocytochrome c-553
show the reaction diagram		Desulfovibrio vulgaris--389630-389630?
D-lactate + ferricytochrome c-553pyruvate + ferrocytochrome c-553
show the reaction diagram		Desulfovibrio vulgaris-starting enzyme for carbon and energy metabolism389630--?
D-lactate + methylene bluepyruvate + ?
show the reaction diagram		Desulfovibrio vulgaris--389630--?
D-lactate + nitrotetrazolium bluepyruvate + ?
show the reaction diagram		Desulfovibrio vulgaris--389630--?
DL-2-hydroxybutyrate + ferricytochrome c-5532-ketobutyrate + ferrocytochrome c-553
show the reaction diagram		Desulfovibrio vulgaris--389630-389630?
additional information?-Desulfovibrio vulgaris-no activity with: NAD+, NADP+, FAD, FMN, cytochrome C3 and O2. Enzyme does not have any diaphorase activity389630---

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
D-lactate + ferricytochrome c-553pyruvate + ferrocytochrome c-553
show the reaction diagram		Desulfovibrio vulgaris-starting enzyme for carbon and energy metabolism389630--

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
Ferricytochrome c-553Desulfovibrio vulgaris--389630 2D-image

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
No entries in this field

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
1,10-phenanthrolineDesulfovibrio vulgaris--389630 2D-image
cyanideDesulfovibrio vulgaris--389630 2D-image
PCMBDesulfovibrio vulgaris--389630 2D-image

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
EDTADesulfovibrio vulgaris-slight stimulation389630 2D-image

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.8-D-lactateDesulfovibrio vulgaris--389630 2D-image
2.4-DL-2-HydroxybutyrateDesulfovibrio vulgaris--389630 2D-image

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
8-Desulfovibrio vulgaris-D-lactate + nitrotetrazolium blue389630

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
79Desulfovibrio vulgaris-pH 7.0: about 50% of maximal activity, pH 9.0: about 80% of maximal activity, D-lactate + nitrotetrazolium blue389630

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
No entries in this field

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
No entries in this field

PDBSCOPCATHORGANISM
No entries in this field

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
No entries in this field

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-20°C, 2 days, 20% loss of activityDesulfovibrio vulgaris-389630
4°C, 0.1 M Tris-HCl, pH 7.3, 2 days, 20% loss of activityDesulfovibrio vulgaris-389630

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
partiallyDesulfovibrio vulgaris-389630

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISMLINK TO PUBMEDSOURCE
389630Ogata, M.; Arihara, K.; Yagi, T.D-Lactate dehydrogenase of Desulfovibrio vulgarisJ. Biochem.891423-14311981Desulfovibrio vulgaris PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 1.1.2.5)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)