Information on EC 1.1.1.48 - D-galactose 1-dehydrogenase:

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The expected taxonomic range for this enzyme is: Proteobacteria


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EC NUMBERCOMMENTARY
1.1.1.48-

RECOMMENDED NAMEGeneOntology No.
D-galactose 1-dehydrogenaseGO:0019151

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
D-galactose + NAD+ = D-galactono-1,4-lactone + NADH + H+
show the reaction diagram
B-type enzyme with regard to stereochemistry, transfers hydrogen from carbon-1 of D-galactose to si face of NAD, in the reverse reaction the pro-S hydrogen atom from carbon-4 of NAD(P)H is transferred to the re face of carbon-1 of D-galactono-1,5-lactonePseudomonas fluorescens-286966
D-galactose + NAD+ = D-galactono-1,4-lactone + NADH + H+
show the reaction diagram
B-type enzyme with regard to stereochemistry, transfers hydrogen from carbon-1 of D-galactose to si face of NAD, in the reverse reaction the pro-S hydrogen atom from carbon-4 of NAD(P)H is transferred to the re face of carbon-1 of D-galactono-1,5-lactone; depending on the pH, the immediately formed D-galactono-1,5-lactone rearranges intramolecularly to the corresponding 1,4-lactone or hydrolyzes to D-galctonate or bothPelomonas saccharophila, Pseudomonas fluorescens-286967
D-galactose + NAD+ = D-galactono-1,4-lactone + NADH + H+
show the reaction diagram
depending on the pH, the immediately formed D-galactono-1,5-lactone rearranges intramolecularly to the corresponding 1,4-lactone or hydrolyzes to D-galctonate or bothPelomonas saccharophila-286970
D-galactose + NAD+ = D-galactono-1,4-lactone + NADH + H+
show the reaction diagram
depending on the pH, the immediately formed D-galactono-1,5-lactone rearranges intramolecularly to the corresponding 1,4-lactone or hydrolyzes to D-galctonate or bothPseudomonas fluorescens-286963, 286970
D-galactose + NAD+ = D-galactono-1,4-lactone + NADH + H+
show the reaction diagram
-Pseudomonas fluorescens-655445
D-galactose + NAD+ = D-galactono-1,4-lactone + NADH + H+
show the reaction diagram
----

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
redox reaction----

PATHWAYKEGG LinkMetaCyc Link
galactose degradation II-GALDEG-PWY
Galactose metabolism00052 -
Metabolic pathways01100 -

SYSTEMATIC NAMEIUBMB Comments
D-galactose:NAD+ 1-oxidoreductaseThis enzyme is part of the De Ley-Doudoroff pathway, which is used by some bacteria during growth on D-galactose.

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
beta-galactose dehydrogenase----
beta-galactose dehydrogenasePseudomonas fluorescens--655445
D-galactose dehydrogenase----
D-galactose: NAD+ 1-oxidoreductasePseudomonas fluorescens--655445
GADHPseudomonas fluorescens--655445
NAD-dependent D-galactose dehydrogenase----

CAS REGISTRY NUMBERCOMMENTARY
9028-54-0-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Azotobacter vinelandii-286957--Manually annotated by BRENDA team
Caulobacter crescentus AE22-5089--Manually annotated by BRENDA team
Caulobacter crescentus AE23-5089--Manually annotated by BRENDA team
Caulobacter crescentus CB13-5089--Manually annotated by BRENDA team
Pelomonas saccharophila-286957, 286967, 286968, 286969, 286970, 286971, 286972, 286973, 286974--Manually annotated by BRENDA team
Pseudomonas fluorescens-286955, 286956, 286957, 286958, 286959, 286960, 286962, 286963, 286964, 286966, 286967, 286969, 286970, 286971, 655445--Manually annotated by BRENDA team
Pseudomonas sp.-285776--Manually annotated by BRENDA team
Pseudomonas sp. G6-285776--Manually annotated by BRENDA team
Rhizobium meliloti L5-30-286957, 5093--Manually annotated by BRENDA team
Sinorhizobium meliloti-5093--Manually annotated by BRENDA team
Sinorhizobium meliloti UR23-5093--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
2-deoxy-D-galactose + NAD+2-deoxy-galactono-1,5-lactone + NADH
show the reaction diagram
Pseudomonas fluorescens, Pelomonas saccharophila--286971-286971?
2-deoxy-D-galactose + NAD+2-deoxy-galactono-1,5-lactone + NADH
show the reaction diagram
Pelomonas saccharophila--286973-286973?
2-deoxy-D-galactose + NADP+2-deoxy-galactono-1,5-lactone + NADPH
show the reaction diagram
Pseudomonas fluorescens--286971-286971?
3-deoxy-D-galactose + NAD+3-deoxy-galactono-1,5-lactone + NADH
show the reaction diagram
Pelomonas saccharophila--286973-286973?
4-deoxy-D-galactose + NAD+4-deoxy-galactono-1,5-lactone + NAD(P)H
show the reaction diagram
Pelomonas saccharophila--286973-286973?
6-deoxy-alpha-D-galactose + NAD+6-deoxy-galactono-1,5-lactone + NADH
show the reaction diagram
Pseudomonas fluorescens--286957-286957?
6-deoxy-alpha-D-galactose + NAD+6-deoxy-galactono-1,5-lactone + NADH
show the reaction diagram
Pseudomonas fluorescens--286971-286971?
6-deoxy-alpha-D-galactose + NAD+6-deoxy-galactono-1,5-lactone + NADH
show the reaction diagram
Azotobacter vinelandii, Pelomonas saccharophila--286957-286957?
6-deoxy-alpha-D-galactose + NAD+6-deoxy-galactono-1,5-lactone + NADH
show the reaction diagram
Pelomonas saccharophila--286971-286971?
6-deoxy-alpha-D-galactose + NAD+6-deoxy-galactono-1,5-lactone + NADH
show the reaction diagram
Pelomonas saccharophila--286973-286973?
D-galactopyranose + NAD(P)+D-galactono-1,5-lactone + NAD(P)H
show the reaction diagram
Pseudomonas fluorescens--655445--?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Sinorhizobium meliloti--5093-5093?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Pseudomonas fluorescens--286955-286955?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Pseudomonas fluorescens--286956-286956?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Pseudomonas fluorescens--286957-286957?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Pseudomonas fluorescens--286958-286958?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Pseudomonas fluorescens--286959-286959?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Pseudomonas fluorescens--286960-286960?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Pseudomonas fluorescens--286962-286962?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Pseudomonas fluorescens--286963-286963?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Pseudomonas fluorescens--286964-286964?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Pseudomonas fluorescens--286966-286966?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Pseudomonas fluorescens--286967-286967?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Pseudomonas fluorescens--286969-286969?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Pseudomonas fluorescens--286970-286970?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Pseudomonas fluorescens--286971-286971?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Pseudomonas sp.--285776-285776?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Azotobacter vinelandii, Pelomonas saccharophila--286957-286957?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Pelomonas saccharophila--286967-286967?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Pelomonas saccharophila--286968-286968?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Pelomonas saccharophila--286969-286969?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Pelomonas saccharophila--286970-286970?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Pelomonas saccharophila--286971-286971?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Pelomonas saccharophila--286972-286972?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Pelomonas saccharophila--286973-286973?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Pelomonas saccharophila--286974-286974?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Rhizobium meliloti L5-30--5093-5093?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Rhizobium meliloti L5-30--286957-286957?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Caulobacter crescentus CB13, Caulobacter crescentus AE22, Caulobacter crescentus AE23--5089-5089?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Pseudomonas sp. G6--285776-285776?
D-galactose + NAD+D-galactono-1,5-lactone + NADH
show the reaction diagram
Sinorhizobium meliloti UR23, Sinorhizobium meliloti L5-30--5093-5093?
D-galactose + NADP+D-galactono-1,5-lactone + NADPH
show the reaction diagram
Pseudomonas fluorescens--286957-286957?
D-galactose + NADP+D-galactono-1,5-lactone + NADPH
show the reaction diagram
Rhizobium meliloti L5-30--5093-5093?
D-galactose + NADP+D-galactono-1,5-lactone + NADPH
show the reaction diagram
Rhizobium meliloti L5-30--286957-286957?
L-arabinose + NAD+L-arabinono-1,5-lactone + NADH
show the reaction diagram
Sinorhizobium meliloti--5093-5093?
L-arabinose + NAD+L-arabinono-1,5-lactone + NADH
show the reaction diagram
Pseudomonas fluorescens--286956---
L-arabinose + NAD+L-arabinono-1,5-lactone + NADH
show the reaction diagram
Pseudomonas fluorescens--286957-286957?
L-arabinose + NAD+L-arabinono-1,5-lactone + NADH
show the reaction diagram
Pseudomonas fluorescens--286971-286971?
L-arabinose + NAD+L-arabinono-1,5-lactone + NADH
show the reaction diagram
Pelomonas saccharophila--286957-286957?
L-arabinose + NAD+L-arabinono-1,5-lactone + NADH
show the reaction diagram
Pelomonas saccharophila--286971-286971?
L-arabinose + NAD+L-arabinono-1,5-lactone + NADH
show the reaction diagram
Rhizobium meliloti L5-30--5093-5093?
L-arabinose + NAD+L-arabinono-1,5-lactone + NADH
show the reaction diagram
Rhizobium meliloti L5-30--286957-286957?
L-arabinose + NAD+L-arabinono-1,5-lactone + NADH
show the reaction diagram
Azotobacter vinelandii-1 mM, better substrate than D-galactose286957-286957?
L-arabinose + NAD+L-arabinono-1,5-lactone + NADH
show the reaction diagram
Sinorhizobium meliloti UR23, Sinorhizobium meliloti L5-30--5093-5093?
succinate + NAD(P)+succinic acid anhydride + NAD(P)H
show the reaction diagram
Rhizobium meliloti L5-30--5093-5093?

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY
(Substrate)
LITERATURE
(Substrate)
COMMENTARY
(Product)
LITERATURE
(Product)
REVERSIBILITY
r=reversible
ir=irreversible
?=not specified
No entries in this field

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
NAD+Pseudomonas fluorescens-preferred cofactor655445 2D-image
NADP+Pseudomonas fluorescens--655445 2D-image

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
No entries in this field

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
5,5'-dithiobis(2-nitrobenzoic acid)Pelomonas saccharophila-reacts with first 4 sulfhydryl groups of enzyme, inhibition is completely reversible upon addition of reducing agents, NAD+ and NADH protect against inactivation286972 2D-image
anthraquinone-diamino-benzosulfonyl-dichlorotriazinePseudomonas fluorescens-i.e. Vilmafix Blue A-R, and other derivatives of longer side chain length, inactivation, overview655445 2D-image
p-chloromercuribenzoatePelomonas saccharophila--286972 2D-image

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.185-D-galactosePseudomonas fluorescens-pH 7.5286956 2D-image
0.205-D-galactosePseudomonas fluorescens-pH 8.0286956 2D-image
0.23-D-galactosePseudomonas fluorescens--286955 2D-image
0.255-D-galactosePseudomonas fluorescens-pH 8.5286956 2D-image
0.26-D-galactosePseudomonas fluorescens-pH 7.5, beta-galactosidase-galactose dehydrogenase fusion enzyme286956 2D-image
0.32-D-galactosePseudomonas fluorescens-pH 8.0, beta-galactosidase-galactose dehydrogenase fusion enzyme286956 2D-image
0.37-D-galactosePseudomonas fluorescens-beta-galactosidase-galactose dehydrogenase fusion enzyme, 13 amino acids as linker286955 2D-image
0.4-D-galactosePseudomonas fluorescens-beta-galactosidase-galactose dehydrogenase fusion enzyme, 9 amino acids as linker286955 2D-image
0.43-D-galactosePseudomonas fluorescens-pH 8.5, beta-galactosidase-galactose dehydrogenase fusion enzyme286956 2D-image
0.45-D-galactosePseudomonas fluorescens-beta-galactosidase-galactose dehydrogenase fusion enzyme, 3 amino acids as linker286955 2D-image
0.5-D-galactoseAzotobacter vinelandii--286957 2D-image
0.7-D-galactosePseudomonas fluorescens--286957, 286971 2D-image
1-D-galactosePelomonas saccharophila--286957, 286973 2D-image
0.215-L-arabinosePseudomonas fluorescens-pH 7.5286956 2D-image
0.275-L-arabinosePseudomonas fluorescens-pH 8.0286956 2D-image
0.37-L-arabinosePseudomonas fluorescens-pH 8.5286956 2D-image
0.43-L-arabinosePseudomonas fluorescens-pH 7.5, beta-galactosidase-galactose dehydrogenase fusion enzyme286956 2D-image
0.5-L-arabinosePseudomonas fluorescens-pH 8.0, beta-galactosidase-galactose dehydrogenase fusion enzyme286956 2D-image
0.68-L-arabinosePseudomonas fluorescens-pH 8.5, beta-galactosidase-galactose dehydrogenase fusion enzyme286956 2D-image
0.125-NAD+Azotobacter vinelandii--286957 2D-image
0.14-NAD+Pelomonas saccharophila--286957, 286973 2D-image
0.15-NAD+Pseudomonas fluorescens--286955 2D-image
0.24-NAD+Pseudomonas fluorescens--286957, 286971 2D-image
0.25-NAD+Pseudomonas fluorescens-beta-galactosidase-galactose dehydrogenase fusion enzymes286955 2D-image
2.3-NADP+Pseudomonas fluorescens--286971 2D-image

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
358-D-galactosePelomonas saccharophila--286973 2D-image
1180-D-galactosePseudomonas fluorescens--286971 2D-image

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
0.0074-Sinorhizobium meliloti-substrate combination L-arabinose and D-galactose, coenzyme NADP+, cell free extracts5093
0.008-Rhizobium meliloti L5-30-substrate succinate, coenzyme NADP+, cell free extracts5093
0.009-Caulobacter crescentus AE23-crude extracts, cells grown on D-galactose, inducible enzyme5089
0.012-Rhizobium meliloti L5-30-substrate succinate, coenzyme NAD+, cell free extracts5093
0.023-Caulobacter crescentus AE22-crude extracts, cells grown on D-galactose, inducible enzyme5089
0.026-Caulobacter crescentus CB13-crude extracts, cells grown on D-galactose, inducible enzyme5089
0.031-Rhizobium meliloti L5-30-coenzyme NADP+, cell free extracts5093
0.036-Rhizobium meliloti L5-30-coenzyme NAD+, cell free extracts5093
0.06-Pseudomonas sp.-crude extracts, cells grown on sucrose285776
0.071-Sinorhizobium meliloti-substrate combination L-arabinose and galactose, coenzyme NAD, cell free extracts5093
0.078-Rhizobium meliloti L5-30-substrate L-arabinose, coenzyme NADP+, cell free extracts5093
0.079-Rhizobium meliloti L5-30-substrate L-arabinose, coenzyme NAD+, cell free extracts5093
0.42-Pseudomonas fluorescens-cell extracts286964
17-Pseudomonas fluorescens-beta-galactosidase-galactose dehydrogenase fusion enzyme, 3 amino acids as linker286955
27-Pseudomonas fluorescens-beta-galactosidase-galactose dehydrogenase fusion enzyme, 13 amino acids as linker286955
29-Pseudomonas fluorescens-beta-galactosidase-galactose dehydrogenase fusion enzyme, 9 amino acids as linker286955
34-Pseudomonas fluorescens--286955
36-Rhizobium meliloti L5-30--286957
76-Rhizobium meliloti L5-30-substrate L-arabinose286957
76-Pseudomonas fluorescens-beta-galactosidase-galactose dehydrogenase fusion enzyme286958
202-Pelomonas saccharophila--286957, 286973
260-Azotobacter vinelandii--286957
272-Rhizobium meliloti L5-30-coenzyme NADP+286957
390-Azotobacter vinelandii-substrate L-arabinose286957
849-Pseudomonas fluorescens--286957, 286963, 286971

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
89Pelomonas saccharophila--286973
8.5-Pseudomonas fluorescens-assay at655445
9-Azotobacter vinelandii--286957
9.19.5Pseudomonas fluorescens--286957, 286971
9.9-Pseudomonas fluorescens--286955
10-Pseudomonas fluorescens--286959

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
25-Pseudomonas fluorescens-assay at655445

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
commercial preparationPseudomonas fluorescens-recombinant, purified655445Manually annotated by BRENDA team

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
cytoplasmAzotobacter vinelandii, Pelomonas saccharophila, Pseudomonas fluorescens, Rhizobium meliloti L5-30--5737286957Manually annotated by BRENDA team

PDBSCOPCATHORGANISM
4ew6, downloadSCOP (4ew6)CATH (4ew6)Rhizobium etli (strain CFN 42 / ATCC 51251)

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
64000-Pseudomonas fluorescens-gel filtration286971
74000-Azotobacter vinelandii-gel filtration286957
102000-Pelomonas saccharophila-gel filtration286971
102000-Pelomonas saccharophila--286973

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
tetramerPelomonas saccharophila-alpha4, 4 * 26000, 100 mM phosphate buffer, pH 7.4, 8 M urea, 100 mM 2-mercaptoethanol, sedimentation equilibrium286968
tetramerPelomonas saccharophila--286971
tetramerPelomonas saccharophila-alpha4, 4 * 25000, SDS-PAGE286973
dimerPseudomonas fluorescens-alpha,alpha, 2 * 32000, SDS-PAGE286971
additional informationPseudomonas fluorescens-structure modelling and computational analysis655445

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
4.69.2Pseudomonas fluorescens--286971

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
2065Pelomonas saccharophila-5 min, 100% activity at 57°C, rapid drop above 60°C286973
50-Pseudomonas fluorescens-native enzyme, 70% activity after 20 min, 30% after 60 min, 20% after 90 min, beta-galactosidase-galactose dehydrogenase fusion enzymes, 80% activity after 90 min286955
50-Pseudomonas fluorescens--286958
50-Pseudomonas fluorescens-50% activity after 60 min, 100 mM, pH 8.0, 1 mM MgCl2, 1 mM dithiothreitol286959

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
oxidation of sulhydryl residues inactivates enzyme, reactivation with 100 mM 2-mercaptoethanol or 10 mM dithiothreitol, pH 7.0, 30°C, enzymatically active oligomers of native enzyme are formed upon storage without reducing agentsPelomonas saccharophila-286969, 286972, 286973, 286974

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
4°C, 3.5 M (NH4)2SO4, 20 mM 2-mercaptoethanol, pH 7.0, several months, no loss of activityPelomonas saccharophila-286973
-20°C, 100 mM phosphate buffer, pH 6.5-7.6, 30-50% glycerol, 18 months, no loss of activityPseudomonas fluorescens-286963, 286971

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
Mono Q column, partially purified enzymeAzotobacter vinelandii-286957
ammonium sulfate, heat, phosphate precipitation, DEAE-cellulose, hydroxyapatitePelomonas saccharophila-286973
ammonium sulfate, CM-Sephadex, DEAE-Sephadex, hydroxyapatite, disc electrophoresisPseudomonas fluorescens-286971
ammonium sulfate, DEAE-Sepharose, FPLC-Superose 12, beta-galactosidase-galactose dehydrogenase fusion enzymePseudomonas fluorescens-286958
by affinity chromatography on chimeric mimodye-ligand gel resins, method evaluationPseudomonas fluorescens-655445

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-Pseudomonas fluorescens-286958
beta-galactosidase-galactose dehydrogenase fusion enzymes, expressed in Escherichia coliPseudomonas fluorescens-286955
expressed in Escherichia coli, Escherichia coli beta-galactosidase promotorPseudomonas fluorescens-286964
expressed in Escherichia coli, point mutations in natural promotor increase expressionPseudomonas fluorescens-286960

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
stress treatments, e.g. chilling, heat shock, or high light intensity, enhance L-GalDH-RNA level in leavesLactuca sativa-700792

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
analysisPelomonas saccharophila-determination of galactose in various hepatic diseases and galactosaemia in clinical biochemistry286969
analysisPseudomonas fluorescens-determination of lactose using a biosensor made of immobilized beta-galctosidase-galactose dehydrogenase fusion enzyme286956
analysisPseudomonas fluorescens-determination of galactose in various hepatic diseases and galactosaemia in clinical biochemistry286962, 286969
biotechnologyPseudomonas fluorescens-design and synthesis by protein molecular modelling and ligand docking of several chimeric mimodye-ligands comprising a NAD-pseudomimetic moiety of anthraquinone diaminobenzosulfonic acid and a galactosyl-mimetic moiety of 2-amino-2-deoxygalactose or shikimic acid for usage as tailored ligands in selective affinity chromatography during enzyme purification/production, immobilization on a gel resin, overview655445

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISM (UNIPROT ACCESSION NO.)LINK TO PUBMEDSOURCE
5089Kurn, N.; Contreras, I.; Shapiro, L.Galactose catabolism in Caulobacter crescentusJ. Bacteriol.135517-5201978Caulobacter crescentus AE22, Caulobacter crescentus AE23, Caulobacter crescentus CB13 PubMed
5093Arias, A.; Cervenansky, C.Galactose metabolism in Rhizobium meliloti L5-30J. Bacteriol.1671092-10941986Rhizobium meliloti L5-30, Sinorhizobium meliloti, Sinorhizobium meliloti UR23 PubMed
285776Hu, A.S.L.; Cline, A.L.The regulation of some sugar dehydrogenases in a PseudomonadBiochim. Biophys. Acta93237-2451964Pseudomonas sp., Pseudomonas sp. G6 PubMed
286955Carlsson, H.; Ljung, S.; Bulow, L.Physical and kinetic effects on introduction of various linker regions in beta-galactosidase/galactose dehydrogenase fusion enzymesBiochim. Biophys. Acta1293154-1601996Pseudomonas fluorescens PubMed
286956Carlsson, H.; Ljungcrantz, P.; Lindbladh, C.; Persson, M.; Bulow, L.Use of geneticallly prepared enzyme conjugates in lactose and galactose analysesAnal. Biochem.218278-2831994Pseudomonas fluorescens PubMed
286957Wong, T.Y.; Yao, X.T.The deLey-Doudoroff pathway of galactose metabolism in Azotobacter vinelandiiAppl. Environ. Microbiol.602065-20681994Azotobacter vinelandii, Pelomonas saccharophila, Pseudomonas fluorescens, Rhizobium meliloti L5-30 PubMed
286958Lindbladh, C.; Persson, M.; Bulow, L.; Mosbach, K.Characterization of a recombinant bifunctional enzyme, galactose dehydrogenase/bacterial luciferase, displaying an improved bioluminescence in a three-enzyme systemEur. J. Biochem.204241-2471992Pseudomonas fluorescens PubMed
286959Ljungcrantz, P.; Bulow, L.; Mosbach, K.Construction and characterization of a recombinant tripartite enzyme, galactose dehydrogenase/beta-galactosidase/galactokinaseFEBS Lett.27591-941990Pseudomonas fluorescens PubMed
286960Fiedler, S.; Buckel, P.The D-galactose dehydrogenase gene from Pseudomonas fluorescens: characterization of mutations leading to increased expression in Escherichia coliAppl. Microbiol. Biotechnol.33418-4231990Pseudomonas fluorescens PubMed
286962Fjimura, Y.Fluorometric method with galactose dehydrogenaseMethods Enzymol.6288-2961984Pseudomonas fluorescens-
286963Maier, E.; Kurz, G.D-Galactose dehydrogenase from Pseudomonas fluorescensMethods Enzymol.89176-1811982Pseudomonas fluorescens PubMed
286964Buckel, P.; Zehelein, E.Expression of Pseudomonas fluorescens D-galactose dehydrogenase in E. coliGene16149-1591981Pseudomonas fluorescens PubMed
286966Brendel, K.; Bressler, R.; Alizade, M.A.Stereochemistry of the hydrogen transfer to NAD catalyzed by D-galactose dehydrogenase from Pseudomonas fluorescensBiochim. Biophys. Acta3971-41975Pseudomonas fluorescens PubMed
286967Ueberschär, K.H.; Blachnitzky, E.O.; Lehman, J.; Kurz, G.Stereospecificity of hydrogen transfer catalyzed by D-galactose dehydrogenase from Pseudomonas saccharophila and Pseudomonas fluorescensBiochim. Biophys. Acta39115-181975Pelomonas saccharophila, Pseudomonas fluorescens PubMed
286968Wengenmayer, F.; Kurz, G.Subunit structure of D-galactose dehydrogenase from Pseudomonas SaccharophilaBiochim. Biophys. Acta386590-6021975Pelomonas saccharophila PubMed
286969Kurz, G.; Wallenfels, K.UV-Test mit Galactose DehydrogenaseMethods Enzym. Anal., 3rd Ed. (Bergmeyer, H.U., ed.)21324-13271974Pelomonas saccharophila, Pseudomonas fluorescens-
286970Ueberschär, K.H.; Blachnitzky, E.O.; Kurz, G.Reaction mechanism of D-galactose dehydrogenases from Pseudomonas saccharophila and Pseudomonas fluorescensEur. J. Biochem.48389-4051974Pelomonas saccharophila, Pseudomonas fluorescens PubMed
286971Blachnitzky, E.O.; Wengenmayer, F.; Kurz, G.D-galactose dehydrogenase from Pseudomonas fluorescens. Purification, properties and structureEur. J. Biochem.47235-2501974Pelomonas saccharophila, Pseudomonas fluorescens PubMed
286972Wengenmayer, F.; Ueberschär, K.H.; Kurz, G.Sulfhydryl group reactivity of D-galactose dehydrogenase from Pseudomonas saccharophilaEur. J. Biochem.4349-581974Pelomonas saccharophila PubMed
286973Wengenmayer, f.; Ueberschär, K.H.; Kurz, G.D-Galactose dehydrogenase from Pseudomonas saccharophila. Purification, properties and structureEur. J. Biochem.4049-611973Pelomonas saccharophila PubMed
286974Wengenmayer, F.; Blachnitzky, E.O.; Kurz, G.Artefakt-Formen der D-Galaktose-DehydrogenaseHoppe-Seyler's Z. Physiol. Chem.354131-1351973Pelomonas saccharophila PubMed
655445Mazitsos, C.F.; Rigden, D.J.; Tsoungas, P.G.; Clonis, Y.D.Galactosyl-mimodye ligands for Pseudomonas fluorescens beta-galactose dehydrogenaseEur. J. Biochem.2695391-54052002Pseudomonas fluorescens PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 1.1.1.48)
ExplorEnz
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KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)