Information on EC 1.1.1.31 - 3-hydroxyisobutyrate dehydrogenase:
   PRINT
Please wait a moment until all data are loaded. This message will disappear when all data are loaded.
Mark a special word or phrase in this record:  
Select one or more organisms in this record:

Show additional data Do not include text mining results
Include (text mining) results (more...)
Include results (AMENDA + additional results, but less precise; more...)

Please login to have access to the AMENDA and FRENDA data

EC NUMBERCOMMENTARY
1.1.1.31-

RECOMMENDED NAMEGeneOntology No.
3-hydroxyisobutyrate dehydrogenaseGO:0008442

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
3-hydroxy-2-methylpropanoate + NAD+ = 2-methyl-3-oxopropanoate + NADH + H+
show the reaction diagram		----

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
oxidation----
redox reaction----
reduction----

PATHWAYKEGG LinkMetaCyc Link
valine degradation I-VALDEG-PWY

SYSTEMATIC NAMEIUBMB Comments
3-hydroxy-2-methylpropanoate:NAD+ oxidoreductase-

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
3-HIBADHBacillus cereus--695710
3-hydroxyisobutyrate dehydrogenaseBacillus cereus-cf. EC1.1.1.59, wide substrate specificity (hydroxyacid derivatives)695710
beta-hydroxyisobutyrate dehydrogenase----
HIBADH----
HIBADHHomo sapiens--670284
HIBDHRattus norvegicus--688515

CAS REGISTRY NUMBERCOMMENTARY
9028-39-1-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Bacillus cereus; ATCC 14579; MmsB gene695710--Manually annotated by BRENDA team
Bos taurus-286546--Manually annotated by BRENDA team
Candida parapsilosis-286546--Manually annotated by BRENDA team
Candida rugosa-286551--Manually annotated by BRENDA team
Candida rugosaIFO 0750286547--Manually annotated by BRENDA team
Candida rugosaIFO 0750; IFO 1542286546--Manually annotated by BRENDA team
Corynebacterium hydrocarbons-286546--Manually annotated by BRENDA team
Endomyces reessii-286546--Manually annotated by BRENDA team
Homo sapiens-286550, 670284--Manually annotated by BRENDA team
Micrococcus flavus-286546--Manually annotated by BRENDA team
Micrococcus luteus-286546--Manually annotated by BRENDA team
Nocardia lyena-286546--Manually annotated by BRENDA team
Oryctolagus cuniculus-286544, 286546, 286550, 286551--Manually annotated by BRENDA team
Pseudomonas aeruginosa-286546--Manually annotated by BRENDA team
Pseudomonas dacunhae-286546--Manually annotated by BRENDA team
Pseudomonas putida-286548--Manually annotated by BRENDA team
Pseudomonas putidaE23286551--Manually annotated by BRENDA team
Pseudomonas putidastrain E23, recombinant enzyme expressed in Escherichia coli655051Q59477SwissProtManually annotated by BRENDA team
Pseudomonas putida E23E23286551--Manually annotated by BRENDA team
Pseudomonas putida E23strain E23, recombinant enzyme expressed in Escherichia coli655051Q59477SwissProtManually annotated by BRENDA team
Rattus norvegicus-286550, 286552--Manually annotated by BRENDA team
Rattus norvegicusWistar rats688515--Manually annotated by BRENDA team
Rhodobacter sphaeroides-286523--Manually annotated by BRENDA team
Rhodotorula mucilaginosa-286546--Manually annotated by BRENDA team
Sus scrofa-286546, 286549--Manually annotated by BRENDA team
Trichosporon aculeatum-286546--Manually annotated by BRENDA team
Tsukamurella paurometabola-286546--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
physiological functionBacillus cereus-3-hydroxyisobutyrate dehydrogenase is a key enzyme for the metabolism of valine and some keto-bodies. It can catalyze reversible conversion of 3-hydroxyisobutyrate to methylmalonate semialdehyde695710

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
2-methyl-D,L-serine + NAD+?
show the reaction diagram		Pseudomonas putida--286551--?
3-hydroxy-2-methylpropanoate + NAD+2-methyl-3-oxopropanoate + NADH + H+
show the reaction diagram		Bacillus cereus--695710--r
3-hydroxy-2-methylpropanoate + NADP+2-methyl-3-oxopropanoate + NADPH + H+
show the reaction diagram		Bacillus cereus--695710--r
3-hydroxyisobutyrate + NAD+methylmalonate semialdehyde + NADH + H+
show the reaction diagram		Bacillus cereus--695710--r
3-hydroxyisobutyric acid + NAD+methylmalonic semialdehyde + NADH
show the reaction diagram		Homo sapiens--670284--r
3-hydroxypropionate + NAD(P)+?
show the reaction diagram		Bacillus cereus-is more active with NADP+ than NAD+695710--?
3-hydroxypropionate + NAD+?
show the reaction diagram		Oryctolagus cuniculus--286550, 286551--?
3-hydroxypropionate + NAD+?
show the reaction diagram		Pseudomonas putida--286551--?
3-hydroxypropionate + NAD+malonate semialdehyde + NADH + H+
show the reaction diagram		Bacillus cereus--695710--r
3-hydroxypropionate + NADP+malonate semialdehyde + NADPH + H+
show the reaction diagram		Bacillus cereus--695710--r
D,L-3-hydroxyisobutyrate + NAD+L-2-methyl-3-oxopropionate + NADH
show the reaction diagram		Pseudomonas aeruginosa, Pseudomonas dacunhae, Endomyces reessii, Nocardia lyena--286546--?
D,L-3-hydroxyisobutyrate + NAD+L-2-methyl-3-oxopropionate + NADH
show the reaction diagram		Candida rugosa-strain IFO 1542286546--?
D,L-3-hydroxyisobutyrate + NAD+D-2-methyl-3-oxopropionate + NADH
show the reaction diagram		Candida parapsilosis--286546--?
D,L-3-hydroxyisobutyrate + NAD+D-2-methyl-3-oxopropionate + NADH
show the reaction diagram		Candida rugosa-strain IFO 0750286546--?
D,L-3-hydroxyisobutyrate + NAD+2-methyl-3-oxopropionate + NADH
show the reaction diagram		Rhodobacter sphaeroides--286523--?
L-3-hydroxyisobutyrate + NAD+L-2-methyl-3-oxopropionate + NADH
show the reaction diagram		Rattus norvegicus--286552--?
L-3-hydroxyisobutyrate + NAD+L-2-methyl-3-oxopropionate + NADH
show the reaction diagram		Sus scrofa--286549---
L-3-hydroxyisobutyrate + NAD+L-2-methyl-3-oxopropionate + NADH
show the reaction diagram		Sus scrofa, Bos taurus--286546--?
L-3-hydroxyisobutyrate + NAD+L-2-methyl-3-oxopropionate + NADH
show the reaction diagram		Oryctolagus cuniculus--286546, 286550---
L-3-hydroxyisobutyrate + NAD+L-2-methyl-3-oxopropionate + NADH
show the reaction diagram		Oryctolagus cuniculus--286544, 286551--r
L-3-hydroxyisobutyrate + NAD+L-2-methyl-3-oxopropionate + NADH
show the reaction diagram		Pseudomonas putida--286551--?
L-3-hydroxyisobutyrate + NAD+L-2-methyl-3-oxopropionate + NADH
show the reaction diagram		Micrococcus luteus, Rhodotorula mucilaginosa, Micrococcus flavus, Corynebacterium hydrocarbons, Tsukamurella paurometabola, Trichosporon aculeatum--286546--?
L-3-hydroxyisobutyrate + NAD+3-oxoisobutyrate + NADH
show the reaction diagram		Pseudomonas putidaQ59477high stereospecificity655051--r
L-serine + NAD+?
show the reaction diagram		Pseudomonas putida--286551--?
additional information?-Rattus norvegicus-the enzyme catalyzes the rate-limiting step in the irreversible degradative pathway for the carbon skeleton of valine and the other branched-chain amino acids688515---
additional information?-Bacillus cereus-MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase as well as 3-hydroxypropionate dehydrogenase activity, EC 1.1.1.59, wide substrate specificity of the 3-HIBADH695710---

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
3-hydroxy-2-methylpropanoate + NAD+2-methyl-3-oxopropanoate + NADH + H+
show the reaction diagram		Bacillus cereus--695710--
3-hydroxy-2-methylpropanoate + NADP+2-methyl-3-oxopropanoate + NADPH + H+
show the reaction diagram		Bacillus cereus--695710--
D,L-3-hydroxyisobutyrate + NAD+L-2-methyl-3-oxopropionate + NADH
show the reaction diagram		Pseudomonas aeruginosa, Pseudomonas dacunhae, Endomyces reessii, Nocardia lyena--286546--
D,L-3-hydroxyisobutyrate + NAD+L-2-methyl-3-oxopropionate + NADH
show the reaction diagram		Candida rugosa-strain IFO 1542286546--
D,L-3-hydroxyisobutyrate + NAD+D-2-methyl-3-oxopropionate + NADH
show the reaction diagram		Candida parapsilosis--286546--
D,L-3-hydroxyisobutyrate + NAD+D-2-methyl-3-oxopropionate + NADH
show the reaction diagram		Candida rugosa-strain IFO 0750286546--
D,L-3-hydroxyisobutyrate + NAD+2-methyl-3-oxopropionate + NADH
show the reaction diagram		Rhodobacter sphaeroides--286523--
L-3-hydroxyisobutyrate + NAD+L-2-methyl-3-oxopropionate + NADH
show the reaction diagram		Rattus norvegicus--286552--
L-3-hydroxyisobutyrate + NAD+L-2-methyl-3-oxopropionate + NADH
show the reaction diagram		Sus scrofa, Bos taurus, Oryctolagus cuniculus--286546--
L-3-hydroxyisobutyrate + NAD+L-2-methyl-3-oxopropionate + NADH
show the reaction diagram		Oryctolagus cuniculus--286544, 286551--
L-3-hydroxyisobutyrate + NAD+L-2-methyl-3-oxopropionate + NADH
show the reaction diagram		Pseudomonas putida--286551--
L-3-hydroxyisobutyrate + NAD+L-2-methyl-3-oxopropionate + NADH
show the reaction diagram		Micrococcus luteus, Rhodotorula mucilaginosa, Micrococcus flavus, Corynebacterium hydrocarbons, Tsukamurella paurometabola, Trichosporon aculeatum--286546--
additional information?-Rattus norvegicus-the enzyme catalyzes the rate-limiting step in the irreversible degradative pathway for the carbon skeleton of valine and the other branched-chain amino acids688515--
additional information?-Bacillus cereus-MmsB from Bacillus cereus exhibits 3-hydroxyisobutyrate dehydrogenase as well as 3-hydroxypropionate dehydrogenase activity, EC 1.1.1.59695710--

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
NAD(P)+Bacillus cereus-is more active with NADP+ than NAD+695710 2D-image
NAD+Rhodobacter sphaeroides--286523 2D-image
NAD+Oryctolagus cuniculus--286544, 286546, 286551 2D-image
NAD+Candida rugosa--286546, 286547, 286551 2D-image
NAD+Sus scrofa--286546, 286549 2D-image
NAD+Pseudomonas putida--286551, 655051 2D-image
NAD+Homo sapiens--670284 2D-image
NAD+Bacillus cereus--695710 2D-image
NADHBacillus cereus--695710 2D-image
NADP+Bacillus cereus--695710 2D-image
NADPHBacillus cereus--695710 2D-image
additional informationBacillus cereus-3-HIBADH is more active with NADP+ than NAD+695710-

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
additional informationBacillus cereus-has no metal ion requirement, MnSO4, CuSO4, CaCl2, MgSO4, and FeSO4 have no effect; no metal ion requirement695710

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
(R)-3-hydroxy-2-methylpropanoateOryctolagus cuniculus-competitive inhibitor of the S-isomer286544 2D-image
AgNO3Candida rugosa, Oryctolagus cuniculus, Pseudomonas putida--286551 2D-image
HgCl2Sus scrofa--286549 2D-image
HgCl2Pseudomonas putida--286551 2D-image
NADHOryctolagus cuniculus-product inhibition286544 2D-image
p-chloromercuribenzoateSus scrofa--286549 2D-image
p-chloromercuribenzoateCandida rugosa, Oryctolagus cuniculus, Pseudomonas putida--286551 2D-image
Zn2+Bacillus cereus-inhibits at 0.2 mM695710 2D-image
ZnCl2Bacillus cereus-0.2 mM, 60% inhibition; inhibits approximately 60% of 3-HIBADH activity at 0.2 mM but shows no effect at 0.005 mM695710 2D-image
iodoacetateSus scrofa--286549 2D-image
additional informationBacillus cereus-is not inhibited by 1-propanol, lactate potassium, malate potassium, malonate potassium, acetaldehyde, ethanol, and glycerin695710-

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
additional informationBacillus cereus-ethylene glycol tetraacetic acid or EDTA (all at 0.005 and 0.2 mM) do not affect 3-HIBADH activity695710-

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
83-2-methyl-D,L-serinePseudomonas putida--286551 2D-image
0.12-3-hydroxyisobutyric acidHomo sapiens--670284 2D-image
16.8-3-HydroxypropionateBacillus cereus-; pH 8.5, 37°C, KM-value for 3-hydroxyisobutyrate is approximately 20fold lower695710 2D-image
18-3-HydroxypropionatePseudomonas putida--286551 2D-image
0.1-D,L-3-hydroxyisobutyrateRhodobacter sphaeroides--286523 2D-image
0.12-D,L-3-hydroxyisobutyrateSus scrofa--286549 2D-image
1.25-D-3-hydroxyisobutyrateCandida rugosa--286547, 286551 2D-image
0.056-L-3-hydroxyisobutyrateRattus norvegicus-wild type, NAD as cofactor286552 2D-image
0.061-L-3-hydroxyisobutyrateOryctolagus cuniculus--286550, 286551 2D-image
0.104-L-3-hydroxyisobutyrateRattus norvegicus-wild type, NADP as cofactor286552 2D-image
0.11-L-3-hydroxyisobutyrateRattus norvegicus-mutant D33R, NADP as cofactor286552 2D-image
0.112-L-3-hydroxyisobutyrateRattus norvegicus-mutant D33R, NAD as cofactor286552 2D-image
0.12-L-3-hydroxyisobutyratePseudomonas putida--286551 2D-image
0.37-L-3-hydroxyisobutyrateCandida rugosa--286547, 286551 2D-image
44-L-serinePseudomonas putida--286551 2D-image
0.022-NAD+Rhodobacter sphaeroides--286523 2D-image
0.022-NAD+Rattus norvegicus-wild type286552 2D-image
0.023-NAD+Oryctolagus cuniculus--286550, 286551 2D-image
0.05-NAD+Candida rugosa--286547, 286551 2D-image
0.054-NAD+Sus scrofa--286549 2D-image
0.44-NAD+Pseudomonas putida--286551 2D-image
0.5-NAD+Homo sapiens--670284 2D-image
1.24-NAD+Rattus norvegicus-mutant D33R286552 2D-image
2.4-NAD+Bacillus cereus-; pH 8.5, 37°C695710 2D-image
0.24-NADP+Rattus norvegicus-mutant D33R286552 2D-image
0.25-NADP+Bacillus cereus-; pH 8.5, 37°C695710 2D-image
0.96-NADP+Rattus norvegicus-wild type286552 2D-image

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.49-(R)-3-HydroxyisobutyrateOryctolagus cuniculus--286544 2D-image
7.1-(S)-3-hydroxyisobutyrateOryctolagus cuniculus--286544 2D-image
0.21-3-HydroxypropionateBacillus cereus-; pH 8.5, 37°C695710 2D-image

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
0.0125-3-HydroxypropionateBacillus cereus-pH 8.5, 37°C69571034987

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
0.0037-Homo sapiens-index patient with 3-hydroxyisobutyric acidia670284
0.0042-Homo sapiens-patient 2 with 3-hydroxyisobutyric acidia670284
0.0046-Homo sapiens-patient 1 with 3-hydroxyisobutyric acidia670284
0.091-Bacillus cereus-homogenate695710
8.7-Bacillus cereus-purified enzyme695710
8.77-Bacillus cereus-pH 8.5, 37°C; purified enzyme, pH 8.5, 37°C695710
10.7-Oryctolagus cuniculus--286544
20.9-Sus scrofa--286549
36.1-Candida rugosa--286551
294-Pseudomonas putida--286551

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
8.5-Bacillus cereus-assay at695710
8.6-Rhodobacter sphaeroides--286523
8.89Bacillus cereus-; oxidation of 3-hydroxypropionate by 3-HIBADH695710
910.5Candida rugosa--286551
911Oryctolagus cuniculus--286544, 286551
9-Sus scrofa--286549
10-Pseudomonas putida--286551

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
710Bacillus cereus-; oxidation of 3-hydroxypropionate by 3-HIBADH695710
711Sus scrofa--286549
711.5Oryctolagus cuniculus--286544

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
37-Bacillus cereus-; assay at695710

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
3545Bacillus cereus-shows more than 90% of its optimized activity695710

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
astroglial cellRattus norvegicus-astroglia-rich primary culture from neonatal rats688515Manually annotated by BRENDA team
brainRattus norvegicus--688515Manually annotated by BRENDA team
cell cultureRattus norvegicus-astroglia-rich primary culture from neonatal rats688515Manually annotated by BRENDA team
fibroblastHomo sapiens--670284Manually annotated by BRENDA team
heartSus scrofa--286549Manually annotated by BRENDA team
kidneySus scrofa--286546, 286549Manually annotated by BRENDA team
liverOryctolagus cuniculus--286544, 286546, 286550, 286551Manually annotated by BRENDA team
liverSus scrofa--286546, 286549Manually annotated by BRENDA team
neuronRattus norvegicus--688515Manually annotated by BRENDA team
liverBos taurus--286546Manually annotated by BRENDA team
additional informationRattus norvegicus-cell type-specific enzyme expression analysis, immunofluorescence labeling, overview688515Manually annotated by BRENDA team

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
mitochondrionRattus norvegicus--5739688515Manually annotated by BRENDA team
additional informationRattus norvegicus-subcellular enzyme expression analysis, overview-688515Manually annotated by BRENDA team

PDBSCOPCATHORGANISM
2gf2, downloadSCOP (2gf2)CATH (2gf2)Homo sapiens
2i9p, downloadSCOP (2i9p)CATH (2i9p)Homo sapiens
1wp4, downloadSCOP (1wp4)CATH (1wp4)Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)
2cvz, downloadSCOP (2cvz)CATH (2cvz)Thermus thermophilus (strain HB8 / ATCC 27634 / DSM 579)

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
32000-Bacillus cereus-gel filtration695710
74000-Oryctolagus cuniculus-gel filtration286544
74000-Oryctolagus cuniculus--286551
80000-Candida rugosa-gel filtration286547
80000-Candida rugosa--286551
120000-Pseudomonas putida-gel filtration286551

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
?Pseudomonas putidaQ59477x * 30280, deduced from gene sequence, x * 30300, SDS-PAGE655051
?Bacillus cereus-x * 32000, SDS-PAGE695710
homodimerOryctolagus cuniculus-alpha2, 2 * 34000, SDS-PAGE286544
homodimerCandida rugosa-alpha2, 2 * 40000, SDS-PAGE286547
tetramerPseudomonas putida-alpha4, 4 * 30000, SDS-PAGE286551
homodimerCandida rugosa, Oryctolagus cuniculus--286551
additional informationRhodobacter sphaeroides-hydroxybutyrate dehydrogenase and 3-hydroxyisobutyrate dehydrogenase activity286523

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
powdered ammonium sulfate is added to the enzyme solution until it becomes slightly turbid, after 4 days crystallization is completeCandida rugosa-286547

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
4.57.3Candida rugosa--286551
5.79.3Pseudomonas putida--286551
711.5Oryctolagus cuniculus--286551

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
45-Bacillus cereus-enzyme activity is not decreased during preincubation at 45°C within 30 min, but preincubation at 55°C in 3 min can significantly denaturate and deactivate the enzyme; stable at 45°C for 30 min, about 40% activity after 3 min at 55°C695710

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-20°C, 50 mM Tris-HCl buffer, pH 8.5, 2 mM EDTA, 2 mM DTT, 50% glycerolBacillus cereus-695710

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
96fold purified at 4°C by ammonium sulfate fractionation and gel filtration; ammonium sulfate precipitation, hydrophobic interaction chromatography (Phenyl-Sepharose), anion exchange chromatography; native enzyme 100fold by ammonium sulfate fractionation, and hydrophobic interaction and anion exchange chromatographyBacillus cereus-695710
-Candida rugosa-286546, 286547
-Homo sapiens-286550
affinity chromatographyHomo sapiens-670284
-Oryctolagus cuniculus-286544, 286550
-Pseudomonas putida-286548, 286551, 655051
-Rattus norvegicus-286550
-Sus scrofa-286549

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
expressed in Escherichia coli BL21 (DE3); MmsB gene, overexpression in Escherichia coli strain BL21, subcloning in strain DH5alpha; pEB81 recombinant vector introduced into Escherichia coli BL21 to overexpress 3-HIBADH proteinBacillus cereus-695710
expressed in Escherichia coliHomo sapiens-670284
expression in Escherichia coliHomo sapiens-286550
-Pseudomonas putidaQ59477655051
expression in Escherichia coliRattus norvegicus-286550

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
D33RRattus norvegicus-higher affinity to cofactor NADP+ than to natural cofactor NAD+286552

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
additional informationBacillus cereus-3-HIBADH may play a role in biosynthesis of 3-hydroxypropionate as a biological source695710

DISEASETITLE OF PUBLICATIONLINK TO PUBMED
Gastroesophageal RefluxDuodenal lipid-induced symptom generation in gastroesophageal reflux disease: role of apolipoprotein A-IV and cholecystokinin. PubMed

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISMLINK TO PUBMEDSOURCE
286523Worral, E.B.; Gassain, S.; Cox, D.J.; Sugden, M.C.; Palmer, T.N.3-Hydroxyisobutyrate dehydrogenase, an impurity in commercial 3-hydroxybutyrate dehydrogenaseBiochem. J.241297-3001987Rhodobacter sphaeroides PubMed
286544Rougraff, P.M.; Paxton, R.; Kuntz, M.J.; Crabb, D.W.; Harris, R.APurification and characterization of 3-hydroxyisobutyrate dehydrogenase from rat liverJ. Biol. Chem.263327-3311988Oryctolagus cuniculus PubMed
286546Hasegawa, JDistribution in organisms and stereospecificity of beta-hydroxyisobutyrate dehydrogenaseAgric. Biol. Chem.452899-29011981Bos taurus, Candida parapsilosis, Candida rugosa, Corynebacterium hydrocarbons, Endomyces reessii, Micrococcus flavus, Micrococcus luteus, Nocardia lyena, Oryctolagus cuniculus, Pseudomonas aeruginosa, Pseudomonas dacunhae, Rhodotorula mucilaginosa, Sus scrofa, Trichosporon aculeatum, Tsukamurella paurometabola-
286547Hasegawa, JPurification, crystallization and some properties of beta-hydroxyisobutyrate dehydrogenase from candida rugosa IFO 0750Agric. Biol. Chem.452805-28141981Candida rugosa-
286548De Paul Marshall, V.; Sokatch, J.R.Regulation of valine catabolism in Pseudomonas putidaJ. Bacteriol.1101073-10811972Pseudomonas putida PubMed
286549Robinson, W.G.; Coon, M.J.The purification and properties of beta-hydroxyisobutyrate dehydrogenaseJ. Biol. Chem.225511-5211956Sus scrofa-
286550Hawes, J.W.; Crabb, D.W.; Chan, R.J.; Rougraff, P.M.; Paxton, R.; Harris, R.A.Mammalien 3-hydroxyisobutyrate dehydrogenaseMethods Enzymol.324218-2282000Homo sapiens, Oryctolagus cuniculus, Rattus norvegicus PubMed
286551Chowdhury, E.K.; Nagata, S.; Misono, H.3-Hydroxyisobutyrate dehydrogenase from Pseudomonas putida E23: purification and characterizationBiosci. Biotechnol. Biochem.602043-20471996Candida rugosa, Oryctolagus cuniculus, Pseudomonas putida PubMed
286552Hawes, J.W.; Harper, E.T.; Crabb, D.W.; Harris, R.A.Structural and mechanistic aspects of a new family of dehydrogenases, the beta-hydroxyacid dehydrogenasesAdv. Exp. Med. Biol.414395-4021996Rattus norvegicus-
655051Chowdhury, E.K.; Akaishi, Y.; Nagata, S.; Misono, H.Cloning and overexpression of the 3-hydroxyisobutyrate dehydrogenase gene from Pseudomonas putida E23Biosci. Biotechnol. Biochem.67438-4412003Pseudomonas putida PubMed
670284Loupatty, F.J.; van der Steen, A.; Ijlst, L.; Ruiter, J.P.; Ofman, R.; Baumgartner, M.R.; Ballhausen, D.; Yamaguchi, S.; Duran, M.; Wanders, R.J.Clinical, biochemical, and molecular findings in three patients with 3-hydroxyisobutyric aciduriaMol. Genet. Metab.87243-2482006Homo sapiens PubMed
688515Murin, R.; Schaer, A.; Kowtharapu, B.S.; Verleysdonk, S.; Hamprecht, B.Expression of 3-hydroxyisobutyrate dehydrogenase in cultured neural cellsJ. Neurochem.1051176-11862008Rattus norvegicus PubMed
695710Yao, T.; Xu, L.; Ying, H.; Huang, H.; Yan, M.The catalytic property of 3-hydroxyisobutyrate dehydrogenase from Bacillus cereus on 3-hydroxypropionateAppl. Biochem. Biotechnol.160694-7032009Bacillus cereus PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 1.1.1.31)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)