Information on EC 1.1.1.116 - D-arabinose 1-dehydrogenase:
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EC NUMBERCOMMENTARY
1.1.1.116-

RECOMMENDED NAMEGeneOntology No.
D-arabinose 1-dehydrogenaseGO:0047816

REACTIONREACTION DIAGRAMCOMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
D-arabinose + NAD+ = D-arabinono-1,4-lactone + NADH + H+
show the reaction diagram		----

REACTION TYPEORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
oxidation----
redox reaction----
reduction----

PATHWAYKEGG LinkMetaCyc Link
No entries in this field

SYSTEMATIC NAMEIUBMB Comments
D-arabinose:NAD+ 1-oxidoreductase-

SYNONYMSORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
ARASaccharomyces cerevisiae--654283, 668641
ARA1Saccharomyces cerevisiae--695806
Ara2pSaccharomyces cerevisiaeQ04212-668641
arabinose(fucose)dehydrogenase----
AraDHSulfolobus solfataricus--681445
D-arabinose dehydrogenaseSaccharomyces cerevisiae--654283
D-arabinose dehydrogenasePhycomyces blakesleeanus--668709
D-arabinose dehydrogenaseSulfolobus solfataricus--681445
dehydrogenase, D-arabinose----
NAD(+)-specific D-arabinose dehydrogenaseSaccharomyces cerevisiaeQ04212-668641
NAD-pentose-dehydrogenase----

CAS REGISTRY NUMBERCOMMENTARY
37250-47-8-

ORGANISMCOMMENTARYLITERATURESEQUENCE CODESEQUENCE DB SOURCE
Candida albicans-10663--Manually annotated by BRENDA team
Neurospora crassamutants col-15a and col-16a; wild type strain 74A285771--Manually annotated by BRENDA team
Neurospora crassawild type strain 74A285768--Manually annotated by BRENDA team
Phycomyces blakesleeanus-668709--Manually annotated by BRENDA team
Pseudomonas sp.-285772--Manually annotated by BRENDA team
Saccharomyces cerevisiae-285774--Manually annotated by BRENDA team
Saccharomyces cerevisiaeARA2; strain YPH250, gene YMR041c or ARA2, isozyme Ara2p668641Q04212SwissProtManually annotated by BRENDA team
Saccharomyces cerevisiaeLH1695806--Manually annotated by BRENDA team
Saccharomyces cerevisiaerecombinant enzyme overexpressed in Saccharomyces cerevisiae and Zygosaccharomyces bailii; strain GRF18U, gene ARA1654283--Manually annotated by BRENDA team
Saccharomyces cerevisiae GRF18Ustrain GRF18U, gene ARA1654283--Manually annotated by BRENDA team
Saccharomyces cerevisiae LH1LH1695806--Manually annotated by BRENDA team
Saccharomyces cerevisiae YPH250ARA2; strain YPH250, gene YMR041c or ARA2, isozyme Ara2p668641Q04212SwissProtManually annotated by BRENDA team
Sulfolobus solfataricus-681445--Manually annotated by BRENDA team
Sus scrofa-285767, 285769, 285770--Manually annotated by BRENDA team

GENERAL INFORMATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SUBSTRATEPRODUCT                      REACTION DIAGRAMORGANISM UNIPROT ACCESSION NO. COMMENTARY/
Substrate		 LITERATURE/
Substrate		 COMMENTARY/
Product		 LITERATURE/
Product		 Reversibility
r=reversible
ir=irreversible
?=not specified
2-deoxy-D-ribose + NAD+2-deoxy-D-ribono-1,4-lactone + NADH
show the reaction diagram		Sus scrofa--285767--?
2-deoxy-D-ribose + NAD+2-deoxy-D-ribono-1,4-lactone + NADH
show the reaction diagram		Neurospora crassa-no substrate for fungal enzyme285768--?
D-arabinose + NAD+D-arabinono-1,4-lactone + NADH
show the reaction diagram		Sus scrofa--285767--?
D-arabinose + NAD+D-arabinono-1,4-lactone + NADH
show the reaction diagram		Sus scrofa--285769-285769?
D-arabinose + NAD+D-arabinono-1,4-lactone + NADH
show the reaction diagram		Saccharomyces cerevisiae--285774-285774ir
D-arabinose + NAD+D-arabinono-1,4-lactone + NADH
show the reaction diagram		Neurospora crassa--285768-285768?
D-arabinose + NAD+D-arabinono-1,4-lactone + NADH
show the reaction diagram		Neurospora crassa--285771--?
D-arabinose + NAD+D-arabinono-1,4-lactone + NADH
show the reaction diagram		Pseudomonas sp.--285772--?
D-arabinose + NAD+D-arabinono-1,4-lactone + NADH
show the reaction diagram		Candida albicans--10663--ir
D-arabinose + NAD+D-arabinono-1,4-lactone + NADH
show the reaction diagram		Sus scrofa-by far the best substrate285770-285770?, ir
D-arabinose + NAD+D-arabinono-1,4-lactone + NADH
show the reaction diagram		Sus scrofa-irreversible reaction at pH 10285770-285770?, ir
D-arabinose + NAD+D-arabinono-1,4-lactone + NADH
show the reaction diagram		Sus scrofa-one of a variety of NAD+ or NADP+ requiring arabinose dehydrogenases, see also 1.1.1.115285769--?
D-arabinose + NAD+D-arabino-1,4-lactone + NADH
show the reaction diagram		Saccharomyces cerevisiae--654283--?
D-arabinose + NAD+D-arabino-1,4-lactone + NADH
show the reaction diagram		Phycomyces blakesleeanus-the enzyme is involved in the biosynthesis of D-erythroascorbic acid and D-erythroascorbate glucoside from exogeneous D-arabinose in the fungus, overview, D-erythroascorbate is more stable to autoxidation and accumulates in the organism668709--?
D-arabinose + NAD+D-arabino-1,4-lactone + NADH
show the reaction diagram		Saccharomyces cerevisiaeQ04212the enzyme, mainly Ara2p not Ara1p, is involved in the biosynthesis of D-erythroascorbic acid, the five-carbon analog of ascorbic acid, from exogeneous D-arabinose, preferred sugar substrate668641--?
D-arabinose + NAD+D-arabino-1,4-lactone + NADH
show the reaction diagram		Saccharomyces cerevisiae-preferred sugar substrate668641--?
D-arabinose + NADP+D-arabinono-1,4-lactone + NADPH
show the reaction diagram		Saccharomyces cerevisiae--285774--?
D-arabinose + NADP+D-arabinono-1,4-lactone + NADPH
show the reaction diagram		Candida albicans--10663--?
D-arabinose + NADP+D-arabino-1,4-lactone + NADPH + H+
show the reaction diagram		Sulfolobus solfataricus--681445--?
D-arabinose + NADP+D-arabino-1,4-lactone + NADPH
show the reaction diagram		Sulfolobus solfataricus--681445--?
D-glucose + NAD+D-glucono-1,5-lactone + NADH + H+
show the reaction diagram		Neurospora crassa--285768--?
D-lyxose + NAD+D-lyxono-1,5-lactone + NADH
show the reaction diagram		Sus scrofa--285767--?
D-ribose + NAD+D-ribono-1,4-lactone + NADH
show the reaction diagram		Sus scrofa--285767--?
D-ribose + NAD+D-ribono-1,4-lactone + NADH
show the reaction diagram		Neurospora crassa--285768--?
D-ribose + NAD+D-ribono-1,4-lactone + NADH
show the reaction diagram		Sulfolobus solfataricus--681445--?
D-ribose + NAD+D-ribono-1,4-lactone + NADH
show the reaction diagram		Pseudomonas sp.-no substrate285772---
D-ribose + NAD+D-ribono-1,4-lactone + NADH + H+
show the reaction diagram		Sulfolobus solfataricus--681445--?
D-xylose + NAD+D-xylono-1,4-lactone + NADH
show the reaction diagram		Saccharomyces cerevisiae--285774--?
D-xylose + NAD+D-xylono-1,4-lactone + NADH
show the reaction diagram		Neurospora crassa--285768--?
D-xylose + NAD+D-xylono-1,4-lactone + NADH
show the reaction diagram		Sus scrofa-poor substrate285767--?
D-xylose + NAD+D-xylono-1,4-lactone + NADH
show the reaction diagram		Pseudomonas sp.-no substrate for bacterial enzyme285772--?
digitoxose + NAD+?
show the reaction diagram		Sus scrofa--285767--?
L-arabinose + NAD+L-arabinono-1,4-lactone + NADH
show the reaction diagram		Neurospora crassa--285768-285768?
L-arabinose + NAD+L-arabinono-1,4-lactone + NADH
show the reaction diagram		Sus scrofa-poor substrate285767--?
L-arabinose + NAD+L-arabinono-1,4-lactone + NADH
show the reaction diagram		Pseudomonas sp.-no substrate285772---
L-fucose + NAD+L-fuconolactone + NADH
show the reaction diagram		Sus scrofa--285769--?
L-fucose + NAD+L-fuconolactone + NADH
show the reaction diagram		Sus scrofa--285770-285770?
L-fucose + NAD+L-fuconolactone + NADH
show the reaction diagram		Neurospora crassa--285768--?
L-fucose + NAD+L-fuconolactone + NADH
show the reaction diagram		Pseudomonas sp.--285772---
L-fucose + NAD+L-fuconolactone + NADH
show the reaction diagram		Saccharomyces cerevisiae-with NADP+285774--?
L-fucose + NAD+L-fuconolactone + NADH
show the reaction diagram		Candida albicans-with NADP+10663--?
L-fucose + NAD+L-6-deoxyascorbate + NADH
show the reaction diagram		Phycomyces blakesleeanus--668709--?
L-fucose + NAD+?
show the reaction diagram		Saccharomyces cerevisiaeQ04212-668641--?
L-fucose + NADP+L-fuconolactone + NADH + H+
show the reaction diagram		Sulfolobus solfataricus--681445--?
L-fucose + NADP+L-fuconolactone + NADPH
show the reaction diagram		Sulfolobus solfataricus--681445--?
L-galactose + NAD+L-ascorbate + NADH
show the reaction diagram		Phycomyces blakesleeanus--668709--?
L-galactose + NAD+?
show the reaction diagram		Saccharomyces cerevisiaeQ04212-668641--?
L-galactose + NAD+? + NADH
show the reaction diagram		Sulfolobus solfataricus--681445--?
L-galactose + NADP+L-galactono-1,5-lactone + NADH
show the reaction diagram		Saccharomyces cerevisiae--285774--?
L-galactose + NADP+L-galactono-1,5-lactone + NADH
show the reaction diagram		Candida albicans--10663--?
L-galactose + NADP+L-ascorbate + NADH + H+
show the reaction diagram		Sulfolobus solfataricus--681445--?
L-xylose + NAD+?
show the reaction diagram		Saccharomyces cerevisiaeQ04212-668641--?
L-xylose + NADP+L-xylono-1,4-lactone + NADH
show the reaction diagram		Saccharomyces cerevisiae--285774--?
L-xylose + NADP+L-xylono-1,4-lactone + NADH
show the reaction diagram		Candida albicans--10663--?
additional information?-Neurospora crassa-no substrates for the fungal enzyme are D-fucose, L-xylose, 6-phosphogluconate285768---
additional information?-Pseudomonas sp.-no substrates for the bacterial enzyme are D-lyxose, D-fucose, L-rhamnose, 2-deoxy-D-glucose, D-fructose, sucrose, xylitol, L-arabitol, D-arabitol, ribitol, D-sorbitol, D-mannitol, D-galactose, D-mannose285772---
additional information?-Saccharomyces cerevisiaeQ04212ARa2p shows a broad sugar substrate specificity, but prefers D-arabinose668641---

NATURAL SUBSTRATESNATURAL PRODUCTSREACTION DIAGRAMORGANISM UNIPROT ACCESSION NO.COMMENTARY SUBSTRATELITERATURE
(Substrate)		COMMENTARY PRODUCTLITERATURE
(Product)
D-arabinose + NAD+D-arabinono-1,4-lactone + NADH
show the reaction diagram		Sus scrofa-one of a variety of NAD+ or NADP+ requiring arabinose dehydrogenases, see also 1.1.1.115285769--
D-arabinose + NAD+D-arabino-1,4-lactone + NADH
show the reaction diagram		Saccharomyces cerevisiae--654283--
D-arabinose + NAD+D-arabino-1,4-lactone + NADH
show the reaction diagram		Phycomyces blakesleeanus-the enzyme is involved in the biosynthesis of D-erythroascorbic acid and D-erythroascorbate glucoside from exogeneous D-arabinose in the fungus, overview, D-erythroascorbate is more stable to autoxidation and accumulates in the organism668709--
D-arabinose + NAD+D-arabino-1,4-lactone + NADH
show the reaction diagram		Saccharomyces cerevisiaeQ04212the enzyme, mainly Ara2p not Ara1p, is involved in the biosynthesis of D-erythroascorbic acid, the five-carbon analog of ascorbic acid, from exogeneous D-arabinose668641--
L-fucose + NAD+L-6-deoxyascorbate + NADH
show the reaction diagram		Phycomyces blakesleeanus--668709--
L-galactose + NAD+L-ascorbate + NADH
show the reaction diagram		Phycomyces blakesleeanus--668709--

COFACTORORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATUREIMAGE
NAD+Sus scrofa--285767, 285769, 285770 2D-image
NAD+Neurospora crassa--285768, 285771 2D-image
NAD+Pseudomonas sp.--285772 2D-image
NAD+Saccharomyces cerevisiae--654283 2D-image
NAD+Saccharomyces cerevisiaeQ04212Ara2p is specific for NAD+, while Ara1p utilizes NADP+668641 2D-image
NAD+Phycomyces blakesleeanus--668709 2D-image
NAD+Sulfolobus solfataricus-; highest activity is observed with L-fucose in combination with NAD+, the remaining sugars are oxidized at higher rates using NADP+681445 2D-image
NADHSulfolobus solfataricus--681445 2D-image
NADP+Candida albicans--10663 2D-image
NADP+Sus scrofa-66% as effective as NAD+285767 2D-image
NADP+Pseudomonas sp.-66% as effective as NAD+; as effective as NAD+285772 2D-image
NADP+Saccharomyces cerevisiae--285774 2D-image
NADP+Sulfolobus solfataricus-; highest activity is observed with L-fucose in combination with NAD+, the remaining sugars are oxidized at higher rates using NADP+681445 2D-image
NADPHSulfolobus solfataricus--681445 2D-image

METALS and IONS ORGANISM UNIPROT ACCESSION NO.COMMENTARY LITERATURE
Zn2+Sulfolobus solfataricus-contains a structural as well as catalytic zinc ion; two ions per subunit681445

INHIBITORSORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
Cu2+Candida albicans--10663 2D-image
D-arabinoseSus scrofa-at high concentrations285770 2D-image
D-arabinoseSaccharomyces cerevisiaeQ04212substrate inhibition of Ara2p at concentrations above 5 mM668641 2D-image
D-glucosePseudomonas sp.-excess concentration285772 2D-image
Fe2+Candida albicans--10663 2D-image
Hg2+Candida albicans--10663 2D-image
HgCl2Sus scrofa--285767 2D-image
iodoacetamideSus scrofa--285767 2D-image
L-fucoseSus scrofa-at high concentrations285770 2D-image
L-XylosePseudomonas sp.-excess concentration285772 2D-image
Mg2+Candida albicans--10663 2D-image
Mn2+Candida albicans--10663 2D-image
N-ethylmaleimideCandida albicans--10663 2D-image
N-ethylmaleimideSus scrofa--285767 2D-image
NADHSus scrofa-non competitive inhibition at non-saturating NAD+-level285770 2D-image
NADPHCandida albicans--10663 2D-image
p-chloromercuribenzoateCandida albicans--10663 2D-image
p-chloromercuribenzoateSus scrofa--285767 2D-image
Zn2+Candida albicans--10663 2D-image

ACTIVATING COMPOUNDORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

KM VALUE [mM]KM VALUE [mM] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
24-2-deoxy-D-riboseSus scrofa--285767 2D-image
0.5-D-arabinosePseudomonas sp.--285772 2D-image
0.67-D-arabinoseNeurospora crassa--285768, 285771 2D-image
0.78-D-arabinoseSaccharomyces cerevisiaeQ04212pH 8.2, 25°C, recombinant His-tagged Ara2p668641 2D-image
1.4-D-arabinoseSulfolobus solfataricus-cosubstrate NADP+, pH 7.5, 70°C; in the presence of 0.4 mM NADP+, in 0.1 M Hepes-KOH buffer (pH 7.5) at 70°C681445 2D-image
19-D-arabinoseSus scrofa--285769 2D-image
20-D-arabinoseSus scrofa--285767 2D-image
29.2-D-arabinoseCandida albicans--10663 2D-image
161-D-arabinoseSaccharomyces cerevisiae--285774 2D-image
670-D-RiboseSus scrofa--285767 2D-image
49-digitoxoseSus scrofa--285767 2D-image
0.18-L-fucosePseudomonas sp.--285772 2D-image
0.22-L-fucoseSulfolobus solfataricus-cosubstrate NADP+, pH 7.5, 70°C; in the presence of 0.4 mM NADP+, in 0.1 M Hepes-KOH buffer (pH 7.5) at 70°C681445 2D-image
5.3-L-fucoseSus scrofa--285769 2D-image
28.2-L-fucoseCandida albicans--10663 2D-image
98-L-fucoseSaccharomyces cerevisiae--285774 2D-image
91.3-L-galactoseCandida albicans--10663 2D-image
180-L-galactoseSaccharomyces cerevisiae--285774 2D-image
24-L-XyloseSaccharomyces cerevisiae--285774 2D-image
37.1-L-XyloseCandida albicans--10663 2D-image
0.017-NAD+Sus scrofa--285767 2D-image
0.024-NAD+Sus scrofa--285769 2D-image
0.087-NAD+Pseudomonas sp.--285772 2D-image
0.3-NAD+Neurospora crassa--285768, 285771 2D-image
1.25-NAD+Sulfolobus solfataricus-in the presence of 10 mM D-arabinose, in 0.1 M HEPES-KOH buffer (pH 7.5) at 70°C681445 2D-image
1.35-NAD+Sulfolobus solfataricus-cosubstrate D-arabinose, pH 7.5, 70°C681445 2D-image
4.1-NAD+Saccharomyces cerevisiaeQ04212pH 8.2, 25°C, recombinant His-tagged Ara2p668641 2D-image
0.038-NADP+Sulfolobus solfataricus-cosubstrate D-arabinose, pH 7.5, 70°C; in the presence of 0.4 mM NADP+, in 0.1 M Hepes-KOH buffer (pH 7.5) at 70°C681445 2D-image
0.048-NADP+Pseudomonas sp.--285772 2D-image

TURNOVER NUMBER [1/s] TURNOVER NUMBER MAXIMUM[1/s] SUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

kcat/KM VALUE [1/mMs-1]kcat/KM VALUE [1/mMs-1] MaximumSUBSTRATEORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

Ki VALUE [mM]Ki VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

IC50 VALUE [mM]IC50 VALUE [mM] MaximumINHIBITORORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE IMAGE
No entries in this field

SPECIFIC ACTIVITY [µmol/min/mg] SPECIFIC ACTIVITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
0.00071-Saccharomyces cerevisiaeQ04212NAD+-dependent Ara2p reaction in presence of 200 mM D-arabinose, native cell extract668641
0.432-Sus scrofa-D-arabinose285769
0.605-Sus scrofa-cosubstrate L-fucose285769
1.11-Neurospora crassa--285771
1.8-Saccharomyces cerevisiaeQ04212purified recombinant His-tagged ARA2p668641
2.2-Candida albicans--10663
101-Saccharomyces cerevisiae--285774
additional information-Saccharomyces cerevisiae--654283

pH OPTIMUMpH MAXIMUMORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
8-Candida albicans--10663
8.2-Saccharomyces cerevisiaeQ04212-668641
8.2-Sulfolobus solfataricus--681445
8.6-Pseudomonas sp.-Tris/HCl buffer285772
9-Neurospora crassa--285771
10-Sus scrofa-glycine/NaOH buffer285769, 285770
10-Saccharomyces cerevisiae--285774
10.6-Sus scrofa--285767

pH RANGEpH RANGE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
6.59Saccharomyces cerevisiaeQ0421220% of maximal activity at pH 6.5 and pH 9.0, pH profile668641
7.39.3Sulfolobus solfataricus-more than 50% active in a pH range from 7.3 to 9.3681445
8.510.5Sus scrofa-about 75% of maximal activity at pH 8.5 and 10.5285769
9.411.8Sus scrofa-about 60% of maximal activity at pH 9.4 and 11.8285767

TEMPERATURE OPTIMUMTEMPERATURE OPTIMUM MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
25-Sus scrofa-assay at285767
25-Saccharomyces cerevisiaeQ04212assay at668641
30-Candida albicans-assay at10663
30-Sus scrofa-assay at285769, 285770
30-Neurospora crassa-assay at285771
30-Pseudomonas sp.-assay at285772
30-Saccharomyces cerevisiae-assay at285774
91-Sulfolobus solfataricus--681445

TEMPERATURE RANGE TEMPERATURE MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

pI VALUEpI VALUE MAXIMUMORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
No entries in this field

SOURCE TISSUE ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE SOURCE
myceliumPhycomyces blakesleeanus--668709Manually annotated by BRENDA team
liverSus scrofa--285767, 285769, 285770Manually annotated by BRENDA team
additional informationSaccharomyces cerevisiae-ARA1 can only be detected in yeast peptone dextrose 2-DE gel images, thus higher reductive efficiency and higher cofactor availability are obtained with the alternation of cultivation condition (mainly growth medium)695806Manually annotated by BRENDA team

LOCALIZATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY GeneOntology No. LITERATURE SOURCE
No entries in this field

PDBSCOPCATHORGANISM
No entries in this field

MOLECULAR WEIGHT MOLECULAR WEIGHT MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
41000-Candida albicans-gel filtration10663
42000-Saccharomyces cerevisiaeQ04212recombinant Ara2p, gel filtration668641
60000-Neurospora crassa-PAGE285771
74000-Saccharomyces cerevisiae-gel filtration285774
245000-Sus scrofa-gel filtration285769

SUBUNITS ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
dimerSaccharomyces cerevisiae-1 * 39000 + 1 * 40000, SDS-PAGE285774
homotetramerSulfolobus solfataricus-x-ray crystallography681445
monomerCandida albicans-1 * 42000, SDS-PAGE10663
monomerNeurospora crassa-1 * 52000, SDS-PAGE, PAGE with 6.2 M urea285771
monomerSaccharomyces cerevisiaeQ042121 * 42400, recombinant Ara2p, SDS-PAGE668641
monomerPhycomyces blakesleeanus-1 * 41000, SDS-PAGE668709
tetramerSulfolobus solfataricus-crystallization data681445

POSTTRANSLATIONAL MODIFICATION ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

Crystallization/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
diffraction to 1.8 A. Structure reveals a catalytic and a cofactor binding domain. Presence of a phosphate binding pocket motif; hanging drop vapour diffusion method with in 0.1 M Hepes-NaOH (pH 8.55) and 10% (v/v) Jeffamine M-600Sulfolobus solfataricus-681445

pH STABILITYpH STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
4.57.5Candida albicans--10663
78Pseudomonas sp.--285772

TEMPERATURE STABILITYTEMPERATURE STABILITY MAXIMUM ORGANISM UNIPROT ACCESSION NO. COMMENTARYLITERATURE
50-Neurospora crassa-half-life of partially purified enzyme 2.25 to 2.4 min285771
7090Sulfolobus solfataricus-the enzyme is thermostable at physiological operating temperatures of 70 and 80°C, displays a half-life of 42 min at 85°C and 26 min at 90°C681445
85-Sulfolobus solfataricus-half-life 42 min681445
90-Sulfolobus solfataricus-half-life 26 min681445

GENERAL STABILITYORGANISM UNIPROT ACCESSION NO.LITERATURE
ammonium sulfate precipitation, almost complete inactivationNeurospora crassa-285768
freeze-drying: inactivationNeurospora crassa-285768
redissolution of (NH4)2SO4-precipitates, complete inactivationSus scrofa-285769

ORGANIC SOLVENT ORGANISM UNIPROT ACCESSION NO. COMMENTARY LITERATURE
No entries in this field

OXIDATION STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

STORAGE STABILITY ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

Purification/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
-Candida albicans-10663
col-16a; mutants col-15aNeurospora crassa-285771
partialNeurospora crassa-285768
native enzyme to near homogeneityPhycomyces blakesleeanus-668709
-Pseudomonas sp.-285772
-Saccharomyces cerevisiae-285774
recombinant His6-tagged Ara2p from Escherichia coli by anion exchange and nickel affinity chromatography to homogeneitySaccharomyces cerevisiaeQ04212668641
Matrex Red A affinity column chromatographySulfolobus solfataricus-681445
-Sus scrofa-285769
partialSus scrofa-285767

Cloned/COMMENTARY ORGANISM UNIPROT ACCESSION NO. LITERATURE
gene ARA1, co-overexpression of the enzyme and the D-arabinono-1,4-lactone oxidase and/or L-galactono-1,4-lactone dehydrogenase in Saccharomyces cerevisiae strains result in overproduction and accumulation of vitamin C in the culture medium, while overexpression of the D-arabinose dehydrogenase alone does not alter the L-ascorbate content, overviewSaccharomyces cerevisiae-654283
gene YMR041c or ARA2, phylogenetic tree, expression of His6-tagged Ara2p in Escherichia coliSaccharomyces cerevisiaeQ04212668641
expressed in Escherichia coli strain HB101Sulfolobus solfataricus-681445

EXPRESSION ORGANISM UNIPROT ACCESSION NO. LITERATURE
No entries in this field

ENGINEERINGORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
additional informationSaccharomyces cerevisiaeQ04212ARA2 gene disruption leads to complete loss of NAD+-specific D-arabinose dehydrogenase activity668641

Renatured/COMMENTARYORGANISM UNIPROT ACCESSION NO.LITERATURE
No entries in this field

APPLICATIONORGANISM UNIPROT ACCESSION NO.COMMENTARYLITERATURE
synthesisSaccharomyces cerevisiae-production of L-ascorbic acid and secretion into culture medium by overexpression of enzyme and arabinone-1,4-lactone oxidase in Saccharomyces cerevisiae and Zygosaccharomyces bailii654283

REF. AUTHORS TITLE JOURNAL VOL. PAGES YEAR ORGANISMLINK TO PUBMEDSOURCE
10663Kim, S.T.; Huh, W.K.; Kim, J.Y.; Hwang, S.W.; Kang, S.O.D-Arabinose dehydrogenase and biosynthesis of erythroascorbic acid in Candida albicansBiochim. Biophys. Acta12971-81996Candida albicans PubMed
285767Schiwara, H.W.; Domschke, W.; Domagk, G.F.Differenzierung verschiedener Zucker-Dehydrogenasen in der Schweineleber durch Disk-Elektrophorese und IonenaustauschchromatograpieHoppe-Seyler's Z. Physiol. Chem.3491575-15811968Sus scrofa PubMed
285768Pincheira G.; Leon G.; Ureta, T.Aldosugar dehydrogenases from Neurospora crassa. Partial purification and characterization of D-arabinose:NAD dehydrogenaseFEBS Lett.30111-1141973Neurospora crassa PubMed
285769Maijub, A.G.; Pecht, M.A.; Miller, G.R.; Carper, W.R.Arabinose (fucose) dehydrogenase from pig liver. I. Isolation and characterizationBiochim. Biophys. Acta31537-421973Sus scrofa PubMed
285770Carper, W.R.; Chang, K.W.; Thorpe, W.G.; Carper, M.A.; Buess, C.M.Arabinose (fucose) dehydrogenase from pig liver. II. Steady-state kineticsBiochim. Biophys. Acta35849-561974Sus scrofa PubMed
285771Carrasco, A.; Pincheira, G.; Ureta, T.Genetic and biochemical characterization of D-arabinose dehydrogenase from Neurospora crassaJ. Bacteriol.145164-1701981Neurospora crassa PubMed
285772Yamanaka, K.Specific microassay of D-arabinose and L-fucose with D-arabinose (L-fucose) dehydrogenaseAgric. Biol. Chem.392227-22341975Pseudomonas sp.-
285774Kim, S.T.; Huh, W.K.; Lee, B.H.; Kang, S.O.D-Arabinose dehydrogenase and its gene from Saccharomyces cerevisiaeBiochim. Biophys. Acta142929-391998Saccharomyces cerevisiae PubMed
654283Sauer, M.; Branduardi, P.; Valli, M.; Porro, D.Production of L-ascorbic acid by metabolically engineered Saccharomyces cerevisiae and Zygosaccharomyces bailiiAppl. Environ. Microbiol.706086-60912004Saccharomyces cerevisiae PubMed
668641Amako, K.; Fujita, K.; Shimohata, T.A.; Hasegawa, E.; Kishimoto, R.; Goda, K.NAD(+)-specific D-arabinose dehydrogenase and its contribution to erythroascorbic acid production in Saccharomyces cerevisiaeFEBS Lett.5806428-64342006Saccharomyces cerevisiae PubMed
668709Baroja-Mazo, A.; del Valle, P.; Rua, J.; de Cima, S.; Busto, F.; de Arriaga, D.; Smirnoff, N.Characterisation and biosynthesis of D-erythroascorbic acid in Phycomyces blakesleeanusFungal Genet. Biol.42390-4022005Phycomyces blakesleeanus PubMed
681445Brouns, S.J.; Turnbull, A.P.; Willemen, H.L.; Akerboom, J.; van der Oost, J.Crystal structure and biochemical properties of the D-arabinose dehydrogenase from Sulfolobus solfataricusJ. Mol. Biol.3711249-12602007Sulfolobus solfataricus PubMed
695806Lin, J.; Liu, Q.; Su, E.; Wei, D.; Yang, S.Application of comparative proteome analysis to reveal influence of cultivation conditions on asymmetric bioreduction of beta-keto ester by Saccharomyces cerevisiaeAppl. Microbiol. Biotechnol.80831-8392008Saccharomyces cerevisiae PubMed

LINKS TO OTHER DATABASES (specific for EC-Number 1.1.1.116)
ExplorEnz
ExPASy
KEGG
MetaCyc
NCBI: PubMed, Protein, Nucleotide, Structure, Genome, OMIM
IUBMB Enzyme Nomenclature
PROSITE Database of protein families and domains
SYSTERS
Protein Mutant Database
InterPro (database of protein families, domains and functional sites)