Resistance to the anti-proliferative activity of recombinant arginine deiminase in cell culture correlates with the endogenous enzyme, argininosuccinate synthetase

Shen, L.J.; Lin, W.C.; Beloussow, K.; Shen, W.C.; Cancer Lett. 191, 165-170 (2003)

 show all sequences of 6.3.4.5

Data extracted from this reference:

Activating Compound
Activating Compound Commentary Organism Structure
additional information study of the effect of recombinant mycoplasma arginine deiminase on cell proliferation, good correlation of the resistance to this treatment with the AS activity in the cell lines Homo sapiens
-
recombinant mycoplasma arginine deiminase 350% increase in AS activity in HeLa cells after rADI treatment, no activity change in umbilical vein endothelial cells Homo sapiens
-
Inhibitors
Inhibitors Commentary Organism Structure
additional information study of the effect of recombinant mycoplasma arginine deiminase on cell proliferation, correlation of the low resistance to this treatment with a low AS activity in the sensitive cell line Canis lupus familiaris
-
additional information study of the effect of recombinant mycoplasma arginine deiminase on cell proliferation, good correlation of the high resistance to this treatment with a high AS activity in the cell line Mus musculus
-
recombinant mycoplasma arginine deiminase 36% decrease in AS activity after rADI treatment Mus musculus
-
Natural Substrates/ Products (Substrates)
Natural Substrates Organism Commentary (Nat. Sub.) Natural Products Commentary (Nat. Pro.) Organism (Nat. Pro.) Reversibility
ATP + L-citrulline + L-aspartate Mus musculus the rate-limiting enzyme for converting citrulline to arginine AMP + diphosphate + L-argininosuccinate
-
 Mus musculus ?
ATP + L-citrulline + L-aspartate Homo sapiens the rate-limiting enzyme for converting citrulline to arginine AMP + diphosphate + L-argininosuccinate
-
 Homo sapiens ?
ATP + L-citrulline + L-aspartate Canis lupus familiaris the rate-limiting enzyme for converting citrulline to arginine AMP + diphosphate + L-argininosuccinate
-
 Canis lupus familiaris ?
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Canis lupus familiaris
-
-
-
Homo sapiens
-
-
-
Mus musculus
-
-
-
Reaction
Reaction Commentary Organism
ATP + L-citrulline + L-aspartate = AMP + diphosphate + 2-(Nomega-L-arginino)succinate
-
 Homo sapiens
ATP + L-citrulline + L-aspartate = AMP + diphosphate + 2-(Nomega-L-arginino)succinate
-
 Mus musculus
ATP + L-citrulline + L-aspartate = AMP + diphosphate + 2-(Nomega-L-arginino)succinate
-
 Canis lupus familiaris
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Commentary Organism
1e-06
-
 37°C, cell homogenate Canis lupus familiaris
6e-06
-
 37°C, A-549 cell homogenate Homo sapiens
1.3e-05
-
 37°C, LNCaP cell homogenate Homo sapiens
1.9e-05
-
 37°C, MCF-7 cell homogenate Homo sapiens
4.8e-05
-
 37°C, HeLa cell homogenate Homo sapiens
0.000174
-
 37°C, cell homogenate Mus musculus
Substrates and Products (Substrate)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
ATP + L-citrulline + L-aspartate catalyzes the coupling reaction between citrulline and arginine to form argininosuccinic acid 650761 Mus musculus AMP + diphosphate + L-argininosuccinate
-
 650761 Mus musculus ?
ATP + L-citrulline + L-aspartate catalyzes the coupling reaction between citrulline and arginine to form argininosuccinic acid 650761 Homo sapiens AMP + diphosphate + L-argininosuccinate
-
 650761 Homo sapiens ?
ATP + L-citrulline + L-aspartate catalyzes the coupling reaction between citrulline and arginine to form argininosuccinic acid 650761 Canis lupus familiaris AMP + diphosphate + L-argininosuccinate
-
 650761 Canis lupus familiaris ?
ATP + L-citrulline + L-aspartate the rate-limiting enzyme for converting citrulline to arginine 650761 Mus musculus AMP + diphosphate + L-argininosuccinate
-
 650761 Mus musculus ?
ATP + L-citrulline + L-aspartate the rate-limiting enzyme for converting citrulline to arginine 650761 Homo sapiens AMP + diphosphate + L-argininosuccinate
-
 650761 Homo sapiens ?
ATP + L-citrulline + L-aspartate the rate-limiting enzyme for converting citrulline to arginine 650761 Canis lupus familiaris AMP + diphosphate + L-argininosuccinate
-
 650761 Canis lupus familiaris ?
Temperature Optimum [°C]
Temperature Optimum [°C] Temperature Optimum Maximum [°C] Commentary Organism
37
-
 assay at Canis lupus familiaris
37
-
 assay at Homo sapiens
37
-
 assay at Mus musculus
Activating Compound (protein specific)
Activating Compound Commentary Organism Structure
additional information study of the effect of recombinant mycoplasma arginine deiminase on cell proliferation, good correlation of the resistance to this treatment with the AS activity in the cell lines Homo sapiens
-
recombinant mycoplasma arginine deiminase 350% increase in AS activity in HeLa cells after rADI treatment, no activity change in umbilical vein endothelial cells Homo sapiens
-
Inhibitors (protein specific)
Inhibitors Commentary Organism Structure
additional information study of the effect of recombinant mycoplasma arginine deiminase on cell proliferation, correlation of the low resistance to this treatment with a low AS activity in the sensitive cell line Canis lupus familiaris
-
additional information study of the effect of recombinant mycoplasma arginine deiminase on cell proliferation, good correlation of the high resistance to this treatment with a high AS activity in the cell line Mus musculus
-
recombinant mycoplasma arginine deiminase 36% decrease in AS activity after rADI treatment Mus musculus
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates Organism Commentary (Nat. Sub.) Natural Products Commentary (Nat. Pro.) Organism (Nat. Pro.) Reversibility
ATP + L-citrulline + L-aspartate Mus musculus the rate-limiting enzyme for converting citrulline to arginine AMP + diphosphate + L-argininosuccinate
-
 Mus musculus ?
ATP + L-citrulline + L-aspartate Homo sapiens the rate-limiting enzyme for converting citrulline to arginine AMP + diphosphate + L-argininosuccinate
-
 Homo sapiens ?
ATP + L-citrulline + L-aspartate Canis lupus familiaris the rate-limiting enzyme for converting citrulline to arginine AMP + diphosphate + L-argininosuccinate
-
 Canis lupus familiaris ?
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Commentary Organism
1e-06
-
 37°C, cell homogenate Canis lupus familiaris
6e-06
-
 37°C, A-549 cell homogenate Homo sapiens
1.3e-05
-
 37°C, LNCaP cell homogenate Homo sapiens
1.9e-05
-
 37°C, MCF-7 cell homogenate Homo sapiens
4.8e-05
-
 37°C, HeLa cell homogenate Homo sapiens
0.000174
-
 37°C, cell homogenate Mus musculus
Substrates and Products (Substrate) (protein specific)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
ATP + L-citrulline + L-aspartate catalyzes the coupling reaction between citrulline and arginine to form argininosuccinic acid 650761 Mus musculus AMP + diphosphate + L-argininosuccinate
-
 650761 Mus musculus ?
ATP + L-citrulline + L-aspartate catalyzes the coupling reaction between citrulline and arginine to form argininosuccinic acid 650761 Homo sapiens AMP + diphosphate + L-argininosuccinate
-
 650761 Homo sapiens ?
ATP + L-citrulline + L-aspartate catalyzes the coupling reaction between citrulline and arginine to form argininosuccinic acid 650761 Canis lupus familiaris AMP + diphosphate + L-argininosuccinate
-
 650761 Canis lupus familiaris ?
ATP + L-citrulline + L-aspartate the rate-limiting enzyme for converting citrulline to arginine 650761 Mus musculus AMP + diphosphate + L-argininosuccinate
-
 650761 Mus musculus ?
ATP + L-citrulline + L-aspartate the rate-limiting enzyme for converting citrulline to arginine 650761 Homo sapiens AMP + diphosphate + L-argininosuccinate
-
 650761 Homo sapiens ?
ATP + L-citrulline + L-aspartate the rate-limiting enzyme for converting citrulline to arginine 650761 Canis lupus familiaris AMP + diphosphate + L-argininosuccinate
-
 650761 Canis lupus familiaris ?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C] Temperature Optimum Maximum [°C] Commentary Organism
37
-
 assay at Canis lupus familiaris
37
-
 assay at Homo sapiens
37
-
 assay at Mus musculus

See also following references to EC number 6.3.4.5 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
715188PrimaInhibition of LPS toxicity by ...Homo sapiens, Mus musculusInt. Immunopharmacol.111180-118820112-2----------6-----4---2-----------2-2-----------------4---2---------11---
715624MunEndothelial argininosuccinate ...Homo sapiensJ. Biol. Chem.2862536-25422011-------------3-----1-----------------------------------1-------------11---
703251Ibarra-GonzalezClinical and biochemical chara ...Homo sapiensClin. Biochem.43461-4662010-------------3-----2-----------------------------------2-------------11---
704078DelageArginine deprivation and argin ...Homo sapiensInt. J. Cancer1262762-27722010-1------2----3-----5----------------1---------2--------5------------3223--
703248LarovereCitrullinemia type I, classica ...Homo sapiensClin. Biochem.421166-11682009----1--------5-----1--------------------1--------------1-------------22---
703594El FadiliDown regulation of KMP-11 in L ...Leishmania infantumExp. Parasitol.12351-572009-------------4------------------------------------------------------1--1--
703969EngelMutations and polymorphisms in ...Homo sapiensHum. Mutat.30300-3072009----21--------3--------------------------21----------------------------22---
704070NicholsonEpigenetic silencing of argini ...Homo sapiensInt. J. Cancer1251454-14632009-1-----------2-----3----------------1------------------3------------1111--
704509GuerreiroArgininosuccinate synthetase i ...Homo sapiens, Mus musculusJ. Biol. Chem.28420022-20033200922-----91-1--7--2--4--1-1---1------22--------91-1----2-4--1-1---1----22---
705506NagasakaEvaluation of endogenous nitri ...Homo sapiensMetab. Clin. Exp.58278-2822009-------------4-----1-----------------------------------1-------------22---
690234KarlbergStructure of human argininosuc ...Homo sapiensActa Crystallogr. Sect. D64279-2862008--111----1-2-3--1-----31-------1-----1111------1-2---1----31--------------
690727GoodwinTroglitazone up-regulates vasc ...Bos taurusBiochem. Biophys. Res. Commun.370254-25820081-1---1--1-1-1-----2--2--------1---1-11----1---1-1-----2--2---------------
690765CorbinPhosphorylation of argininosuc ...Bos taurusBiochem. Biophys. Res. Commun.3771042-10462008--1------1-1-2-11--3--2--------1-----11--------1-1--11-3--2---------------
690768GueiIdentification of a liver-spec ...Homo sapiensBiochem. Biophys. Res. Commun.377257-26120081-----1--1-2-4-----3--3--------1---1--1----1---1-2-----3--3---------------
692607SatohClearance of bacterial lipopol ...Mus musculusInnate Immun.1451-602008--------11-2-3-----2--3--------1------1-------11-2-----2--3---------------
692659BowlesPancreatic cancer cell lines d ...Homo sapiensInt. J. Cancer1231950-19552008------1--1-2-5-----8--2--------1------1----1---1-2-----8--2---------------
693030ZhaoAn NADPH sensor protein (HSCAR ...Homo sapiensJ. Biol. Chem.28311004-110132008--1-1----1-2-2-----2--3-1---1--1-----11-1------1-2-----2--3-1---1---------
671250GoodwinTumor necrosis factor-alpha re ...Bos taurusAm. J. Physiol. Heart Circ. Physiol.293H1115-H11212007-1-----------2-----2----------------1------------------2------------------
673486BizzocoTransient ischemia increases n ...Rattus norvegicusExp. Neurol.204252-2592007-1-----------4-----3----------------1------------------3------------------
674048SzlosarekAberrant regulation of arginin ...Homo sapiensInt. J. Cancer1216-112007-1-----------3-----5----------------1------------------5------------------
676120WagemakerArgininosuccinate synthetase a ...Agaricus bisporusMycol. Res.111493-5022007--1-1-----1--4-----4-----------------1--1-------1------4------------------
676636CruzEnzymatic evidence for the key ...Daucus carota, Glomus intraradicesPlant Physiol.144782-7922007------2------5-----27-1-2---2--------------2-----------27-1-2---2---------
672445Brasse-LagnelBiphasic effect of IL-1beta on ...Homo sapiensBiochimie88607-61220061-----1------3-2---13---1----------1-------1--------2--13---1-------------
674383KimCharacterization of late-onset ...Homo sapiensJ. Biochem. Mol. Biol.39400-4052006-1-----------4----------------------1-------------------------------------
675096SatohLiver argininosuccinate syntha ...Mus musculusJ. Endotoxin Res.1221-382006-1----5--11--4--11-15-1-1---1-----1-1-----15---11----1-15-1-1---1---------
675606Endo-Functional complementation of ...Porphyra yezoensisJ. Phycol.421066-10712006-11-------1--1----------------------11----------1-------------------------
661285Brasse-LagnelIL-1beta stimulates argininosu ...Homo sapiensBiochimie87403-4092005-----------1-4-----1--1--------------------------1-----1--1---------------
662377PendletonRegulation of endothelial argi ...Bos taurusJ. Biol. Chem.28024252-242602005-----------1-2-----3--1--------------------------1-----3--1---------------
662205GoodwinArgininosuccinate synthase exp ...Bos taurusJ. Biol. Chem.27918353-183602004-----------1-2-----3--1--------------------------1-----3--1---------------
662254HaoArgininosuccinate synthetase i ...Homo sapiensJ. Biol. Chem.27936192-362002004--1---1----1-4--1-----1--------------1-----1-----1---1----1---------------
650761ShenResistance to the anti-prolife ...Canis lupus familiaris, Homo sapiens, Mus musculusCancer Lett.191165-17020032-----3----3-4---3--6-6-3----------2-------3-----3------6-6-3-------------
652404GotoStructures of argininosuccinat ...Thermus thermophilusJ. Biol. Chem.27822964-229712003--11-----1-1-5--11--2-21----1--------1-1-------1-1---1--2-21----1---------
653046HukkanenInduction of inducible nitric ...Mus musculusJ. Rheumatol.30652-6592003111--------1-4---1----1------------111-----------1--------1---------------
651195Van GeldreL-citrulline recycling by argi ...Rattus norvegicusEur. J. Pharmacol.455149-1602002------1----1-3---1-1--2--------------------1-----1-----1--2---------------
652231LemkeSubstrate induced conformation ...Escherichia coliJ. Biol. Chem.27713074-130812002--11-------1-3--11----21-------------1-1---------1---1----21--------------
652242GotoCrystal structure of argininos ...Thermus thermophilusJ. Biol. Chem.27715890-158962002--11-----1-1-4--11----21-------------1-1-------1-1---1----21--------------
652976HenekaNeuronal and glial coexpressio ...Homo sapiensJ. Neuropathol. Exp. Neurol.60906-9162001-1---------1-4---1----2-------------1------------1--------2---------------
653186KawaharaCo-induction of argininosuccin ...Mus musculus, Rattus norvegicusMol. Brain Res.90165-173200121---------2-4---2-1--21-----------21------------2-----1--21--------------
649104Lemke-Expression, purification, crys ...Escherichia coliActa Crystallogr. Sect. D552028-20301999--11-------1-1--11----21-------------1-1---------1---1----21--------------
653344HammermannInability of rat alveolar macr ...Rattus norvegicusNaunyn-Schmiedebergs Arch. Pharmacol.358601-60719981----------1-2---1-1--21-----------1-------------1-----1--21--------------
1590IsayamaDistribution and co-localizati ...Felis catusArch. Histol. Cytol.60477-4921997-------------4-----2--1--------------------------------2--1---------------
1591DemarquoyRegulation of argininosuccinat ...Rattus norvegicusMol. Cell. Biochem.14347-511995--------4--1-3--------2-----------------------4--1--------2---------------
1597YuPreparation of recombinant arg ...Rattus norvegicusJ. Biochem.117952-9571995--1----------5--1--2--1--------------1---------------1-2--1---------------
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1593PatejunasGeneration of a mouse model fo ...Mus musculusSomatic Cell Mol. Genet.2055-601994-------------6--------1-----------------------------------1---------------
1598ShaheenCharacterization of human wild ...Homo sapiensEnzyme Protein95251-2641994----4--13--12-4--1-----41----------------4----13--12---1----41--------------
1594Arnt-RamosImmunohistochemical localizati ...Rattus norvegicusNeuroscience51773-7891992-----------1-2-----3--2--------------------------1-----3--2---------------
1596WakuiRenal argininosuccinate synthe ...Sus scrofaRenal Physiol. Biochem.151-91992--------1-1--3--1--1--11----------------------1-1----1-1--11--------------
1595IsahikiIdentification of essential ar ...Homo sapiensProtein Seq. Data Anal.2283-2871989------4------3--------1--------------------4--------------1---------------
1584Saheki-Role of argininosuccinate synt ...Homo sapiens, Rattus norvegicusAdv. Enzyme Regul.18221-2381986-----------1-2--------3--------------------------1--------3---------------
1589KumarArgininosuccinate synthetase: ...Bos taurusProc. Natl. Acad. Sci. USA826745-67491985------1------2---1-1--1--------------------1-----------1--1---------------
1588RaushelNitro analogs of substrates fo ...Bos taurusArch. Biochem. Biophys.232520-5251984-------------2--------1-----------------------------------1---------------
1586SahekiSome properties of argininosuc ...Homo sapiens, Rattus norvegicusJ. Biochem.931531-15371983-------5--1--4--1--21-32---------------------5--1----1-21-32--------------
1587RaushellKinetic mechanism of argininos ...Bos taurusArch. Biochem. Biophys.225979-9851983------4------3---1-2--2--------------------4-----------2--2---------------
1585RatnerArgininosuccinate synthetase o ...Bos taurusProc. Natl. Acad. Sci. USA795197-51991982---1-1--1-1--3-----21-11---------------1-1----1-1------21-11--------------
1583KimballPurification and properties of ...Homo sapiensBiochemistry19705-7091980-------4--1--2--1--21-21---------------------4--1----1-21-21--------------
1578MatsudaStudies on liver argininosucci ...Homo sapiensJ. Biochem.85191-1951979-----1-3--1--1-----1--1---2--------------1---3--1------1--1---2-----------
1579HilgerYeast argininosuccinate synthe ...Saccharomyces cerevisiaeEur. J. Biochem.94153-1631979------3---11-4--11--1221----22-------------3----11---1--1221----22--------
1580TakadaStudies on rat liver argininos ...Rattus norvegicusJ. Biochem.851309-13141979---1--7------2-----2--1----------------1---7-----------2--1---------------
1581O'BrienIsolation and characterization ...Homo sapiensBiochemistry185353-53561979-------3--1--6--1--21111---------------------3--1----1-21111--------------
1582TakadaStudies of rat liver argininos ...Rattus norvegicusJ. Biochem.861353-13591979-----116-----2--1--2--11--1-1------------1-1-6-------1-2--11--1-1---------
1576SahekiStudies of rat liver argininos ...Rattus norvegicusJ. Biochem.81687-6961977---1--66--2--1-----1-131----2----------1---6-6--2------1-131----2---------
1577RochovanskyBiosynthesis of urea. Molecula ...Bos taurusJ. Biol. Chem.2525287-52941977---1--21--1--1--1--11121---------------1---2-1--1----1-11121--------------
1575SahekiCrystallization and some prope ...Rattus norvegicusFEBS Lett.58314-3171975---1---3-----2--1--21-21----1----------1-----3-------1-21-21----1---------
1574RatnerEnzymes of arginine and urea s ...Bos taurusAdv. Enzymol. Relat. Areas Mol. Biol.391-901973---1--63--1--1---1-21-41----2----------1---6-3--1------21-41----2---------
1573Shargool-Purification of argininosuccin ...Pisum sativumPhytochemistry102029-20321971------1------1--1--11-1-----1--------------1---------1-11-1-----1---------