Site-directed mutagenesis of the phosphate-binding consensus sequence in Escherichia coli adenylosuccinate synthetase

Liu, F.; Dong, Q.; Fromm, H.J.; J. Biol. Chem. 267, 2388-2392 (1992)

 show all sequences of 6.3.4.4

Data extracted from this reference:

Engineering
Amino acid exchange Commentary Organism
G12V replacement of Gly12, Gly15, or Gly17 with Val, or replacement of Lys18 with Arg, results in significant decrease in the kcat/Km values of the enzyme Escherichia coli
G17V replacement of Gly12, Gly15, or Gly17 with Val, or replacement of Lys18 with Arg, results in significant decrease in the kcat/Km values of the enzyme Escherichia coli
L18R replacement of Gly12, Gly15, or Gly17 with Val, or replacement of Lys18 with Arg, results in significant decrease in the kcat/Km values of the enzyme Escherichia coli
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Escherichia coli
-
 wild-type and mutant enzymes
-
Substrates and Products (Substrate)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
GTP + IMP + L-Asp
-
 1568 Escherichia coli GDP + phosphate + adenylosuccinate
-
 1568 Escherichia coli
-
Engineering (protein specific)
Amino acid exchange Commentary Organism
G12V replacement of Gly12, Gly15, or Gly17 with Val, or replacement of Lys18 with Arg, results in significant decrease in the kcat/Km values of the enzyme Escherichia coli
G17V replacement of Gly12, Gly15, or Gly17 with Val, or replacement of Lys18 with Arg, results in significant decrease in the kcat/Km values of the enzyme Escherichia coli
L18R replacement of Gly12, Gly15, or Gly17 with Val, or replacement of Lys18 with Arg, results in significant decrease in the kcat/Km values of the enzyme Escherichia coli
Substrates and Products (Substrate) (protein specific)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
GTP + IMP + L-Asp
-
 1568 Escherichia coli GDP + phosphate + adenylosuccinate
-
 1568 Escherichia coli
-

See also following references to EC number 6.3.4.4 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
715099HitomiInosine triphosphate protects ...Homo sapiensGastroenterology1401314-13212011-1-----------4-----1--1-------------1------------------1--1----------11---
714324MehrotraStudies on active site mutants ...Plasmodium falciparumBiochim. Biophys. Acta18041996-200220101-1-7-120--2--5--------12---8----1--1-1--7--1120--2---------12---8----------
709915MillerAdenylosuccinate synthetase 1 ...Mus musculusMol. Carcinog.481116-11222009-------------5-----1-----------------------------------1-------------11---
689055PimkinThe CBS subdomain of inosine 5 ...Escherichia coliMol. Microbiol.68342-3592008----1-1------4------2-1-----------------1--1------------2-1---------------
690685ZhangAn association study of ADSS g ...Homo sapiensBehav. Brain Funct.4392008-1-----------2-----1--1-------------1------------------1--1---------------
691098MehrotraMethanocaldococcus jannaschii ...Methanocaldococcus jannaschiiBiochim. Biophys. Acta17842019-20282008--1------12-12--1-----12--1----------1---------12-1--1----12--1-----------
692460OestreicherThe nadA gene of Aspergillus n ...Emericella nidulansFungal Genet. Biol.45760-77520082------------5--------1------------2----------------------1---------------
695062HonoreIdentification of differential ...Mus musculusProteome Sci.6182008----------1--2-----4--1----------1--------------1------4--1--------1------
673163Li-Comparative molecular characte ...Sus scrofaComp. Biochem. Physiol. B147B271-2772007--1----------2-----11-------------1---2-----------------13-----------1------
672068IancuCavitation as a mechanism of s ...Escherichia coli, Mus musculusBiochemistry4511703-117112006---2--29-----3-----1--4----9----4------2---249---------1--4----9----------
662843SunMolecular cloning and characte ...Homo sapiensMol. Cell. Biochem.26985-942005--1-----1--1-5--1--8--1--------------1--------1--1---1-8--1---------------
652929EaazhisaiCrystal structure of fully lig ...Plasmodium falciparumJ. Mol. Biol.3351251-12642004-111--1----1-5--1-----21------------11-1---1-----1---1----21--------------
662801RamanUnique kinetic mechanism of Pl ...Plasmodium falciparumMol. Biochem. Parasitol.1381-820041-----53-1---4--1-----1----3----4--1-------543-1-----1----1----3----------
652500BorzaVariations in the response of ...Mus musculusJ. Biol. Chem.2786673-66792003--1---612-141-4--1-----21---4----16----1-----61612-141---1----21---4----------
652317IancuFeedback inhibition and produc ...Mus musculusJ. Biol. Chem.27740536-405432002--11--3----1-3--1--1--2--------------1-1---3-----1---1-1--2---------------
652347HouIMP alone organizes the active ...Escherichia coliJ. Biol. Chem.2775970-59762002---1--1----1-3--1-----21---------------1---1-----1---1----21--------------
652356GorrellDeterminants of L-L-aspartate ...Escherichia coliJ. Biol. Chem.2778817-88212002--1-7-339---1-2--1-----31---14----9----1--7--3939---1---1----31---14----------
652608WenThe adenylosuccinate synthetas ...Mus musculus, Rattus norvegicusJ. Cell. Mol. Med.6235-2432002--2--------2-4-----1--4--------------2-----------2-----1--4---------------
653751JayalakshmiPurification and characterizat ...Plasmodium falciparumProtein Expr. Purif.2565-722002-11--1-3--11-4--1---1121----11------11---1---3--11---1--1121----11--------
652193IancuRecombinant mouse muscle adeny ...Mus musculusJ. Biol. Chem.27642146-421522001--11---3---1-6--1--1--3--------------1-1-----3---1---1-1--3---------------
652812PradeStructures of adenylosuccinate ...Arabidopsis thaliana, Triticum aestivumJ. Mol. Biol.296569-5772000--22-------2-6--2-----42-------------2-2---------2---2----42--------------
652965SchmitzerRibofuranosyl triazolone: A na ...Abutilon theophrasti, Alopecurus myosuroides, Amaranthus retroflexus, Arabidopsis thaliana, Avena fatua, Echinochloa crus-galli, Helianthus annuus, Ipomoea hederacea, Setaria faberiJ. Nat. Prod.63777-7812000------9----9-11--------9--------------------9-----9--------9---------------
649264HonzatkoAdenylosuccinate synthetase: R ...Acidithiobacillus ferrooxidans, Arabidopsis thaliana, Azotobacter vinelandii, Bacillus subtilis, Dictyostelium discoideum, Escherichia coli, Haemophilus influenzae, Homo sapiens, Leishmania donovani, Methanocaldococcus jannaschii, Mus musculus, Oryctolagus cuniculus, Pyrococcus sp., Rattus norvegicus, Schizosaccharomyces pombe, Triticum aestivum, Trypanosoma cruzi, Zea maysAdv. Enzymol. Relat. Areas Mol. Biol.7357-1021999--715-23-6-18-20--3-----361---1-----3---7-15--2-3-6-18---3----361---1---3------
649265Honzatko-Adenylosuccinate synthetase: R ...Saccharomyces cerevisiaeAdv. Enzymol. Relat. Areas Mol. Biol.73; 57-102ix-x1999--1--------1-1--------2--------------1-----------1--------2---------------
649423HonzatkoStructure-function studies of ...Escherichia coliArch. Biochem. Biophys.3701-81999---15-2--1-2-3--------21----1---2------15--22--1-2--------21----1---------
649670Lipps-Adenylosuccinate synthase from ...Saccharomyces cerevisiaeBiochem. J.341537-5431999-21--166--31-1--1----131----11--5---21---1-656--31---1---131----11--------
649897LeeImplication of arginine-131 an ...Escherichia coliBiochemistry385754-57631999------1----1-3--------2---------1----------11----1--------2---------------
651921LewisCombinatorial interactions reg ...Mus musculusJ. Biol. Chem.27414188-141971999--1--------1-3-----1--1--------------1-----------1-----1--1---------------
651925HouEffectors of the stringent res ...Escherichia coliJ. Biol. Chem.27417505-175101999--11--2--1-1-4--1-----1--------------1-1---2---1-1---1----1---------------
650258RyzhovaAdenylosuccinate synthetase of ...Saccharomyces cerevisiaeBiochemistry63650-6561998------43---1-3--1-----611---1--------------4-3---1---1----611---1---------
651884WangAmbiguities in mapping the act ...Escherichia coliJ. Biol. Chem.27316000-160041998--116--29--11-4--1-----2----1311-------1-16----29--11---1----2----1311--------
1558PolandEntrapment of 6-thiophosphoryl ...Escherichia coliJ. Biol. Chem.27215200-152051997---1--1------4--------1----------------1---1--------------1---------------
1559KangResidues essential for catalys ...Escherichia coliJ. Biol. Chem.27211881-118851997----3--13-----3--------1----1------------3----13------------1----1----------
1566WangA study of Escherichia coli ad ...Escherichia coliJ. Biol. Chem.2727078-70841997----2--------3--------1-----------------2-----------------1---------------
1567WangRelationship of the conserved ...Escherichia coliJ. Biol. Chem.27216911-169161997----5--16-----3--------1-----2-----------5----16------------1-----2---------
1572WaltersAdenylosuccinate synthetase fr ...Zea maysPlant Physiol.114549-5551997-------3--1--3--1---1-11---11----------------3--1----1--1-11---11---------
1551Fonne-PfisterThe mode of action and the str ...Ocimum basilicumProc. Natl. Acad. Sci. USA939431-94361996---1--1------2--------1----------------1---1--------------1---------------
1554PolandCrystal structures of adenylos ...Escherichia coliJ. Mol. Biol.2641013-10271996---1---------3--------1----------------1------------------1---------------
1555MoeInvolvement of arginine 143 in ...Escherichia coliBiochemistry359024-90331996----1-16-----3--------2----1------------1--1-6------------2----1----------
1557PolandRefined crystal structure of a ...Escherichia coliBiochemistry3515753-157591996---1--51-----3--------1----------------1---5-1------------1---------------
1562KangSubunit complementation of Esc ...Escherichia coliJ. Biol. Chem.27129722-297281996----11-------3--------1-----------------11----------------1---------------
1564PolandRefined crystal structures of ...Escherichia coliJ. Biol. Chem.27115407-154131996---1---------4--------1----1-----------1------------------1----1----------
1570GallertEnzymatic properties and inhib ...Saccharomyces cerevisiaeEur. J. Biochem.239487-4931996------1------6--------1--------------------1--------------1---------------
1571SiehlAdenylosuccinate synthetase: s ...Arabidopsis thaliana, Zea maysPlant Physiol.110753-7581996------4------4--------2--------------------4--------------2---------------
1553SilvaRefined crystal structure of u ...Escherichia coliJ. Mol. Biol.254431-4461995---1---------3--------1----------------1------------------1---------------
1563WangIdentification of arginine res ...Escherichia coliJ. Biol. Chem.27013160-131681995----3--12-----3--1-----1-----------------3----12-------1----1---------------
1565KangIdentification of a essential ...Escherichia coliJ. Biol. Chem.27015539-155441995-------1-3---4--------1----1-----------------1-3----------1----1----------
1556KangCharacterization of the putati ...Escherichia coliArch. Biochem. Biophys.310475-4801994----3--12-----3---1----1----1------------3----12------------1----1----------
1561KangReplacement of Asp333 with Asn ...Escherichia coliJ. Biol. Chem.26924046-240491994----3--16-----3--------4-----------------3----16------------4---------------
1568LiuSite-directed mutagenesis of t ...Escherichia coliJ. Biol. Chem.2672388-23921992----3--------3--------1-----------------3-----------------1---------------
1569PowellCloning and characterization o ...Homo sapiensFEBS Lett.3034-101992--1--------1-5-----1--2--------------1-----------1-----1--2---------------
1552WiesmullerPurification and cDNA-derived ...Dictyostelium discoideumJ. Biol. Chem.2662480-24851991--1----31--1-4--1----12--------------1-------31--1---1---12---------------
1560DongEvidence for an arginine resid ...Escherichia coliJ. Biol. Chem.26612228-122331991----1-16-----3--------1----1------------1--1-6------------1----1----------
1533SerraPreliminary X-ray crystallogra ...Escherichia coliJ. Mol. Biol.200753-7541988---1------11-2--------2----------------1--------11--------2---------------
1534BassOverproduction, purification, ...Escherichia coliArch. Biochem. Biophys.256335-3421987--1----3--12-3--1---1-31-------------1-------3--12---1--1-31--------------
1535CooperIsotope exchange at equilibriu ...Rattus norvegicusBiochemistry257323-73271986-------------3---1-2--1--------------------------------2--1---------------
1536JahngenAdenylosuccinate synthetase fr ...Dictyostelium discoideumArch. Biochem. Biophys.229145-1541984------93-1---2--11--1-1-1---1--------------9-3-1-----1--1-1-1---1---------
1537StaytonRegulation, genetics, and prop ...Azotobacter vinelandii, Bacillus subtilis, Escherichia coli, Gallus gallus, Homo sapiens, Leishmania donovani, Oryctolagus cuniculus, Rattus norvegicus, Schizosaccharomyces pombe, Sus scrofa, Triticum aestivum, Trypanosoma cruziCurr. Top. Cell. Regul.22103-1411983------3247-121--12-----14--22-----1--------------32-47-121------14--22-----1---------
1538PorterNitro analogs of substrates fo ...Azotobacter vinelandiiArch. Biochem. Biophys.225157-1631983------8----1-3--------4--------------------8-----1--------4---------------
1539MatsudaPurification and properties of ...Rattus norvegicusBiochim. Biophys. Acta616340-3501980------173-51--2--1--11111----1-1------------17-3-51----1-11111----1-1-------
1540HaradaPreliminary crystallographic d ...Oryctolagus cuniculusJ. Mol. Biol.132729-7311979---1---------2-----1--1----------------1---------------1--1---------------
1541SpectorSpecificity of adenylosuccinat ...Leishmania donovaniJ. Biol. Chem.2548422-84261979------135--1--2--------5-----1--------------13-5--1---------5-----1---------
1542StaytonGuanosine 5'-diphosphate-3'-di ...Escherichia coliJ. Biol. Chem.2542579-25811979------1------2--------1--------------------1--------------1---------------
1543FischerAdenylosuccinate synthetase (r ...Oryctolagus cuniculusMethods Enzymol.51207-2131978---1--3---1--1--1--41-11----1----------1---3----1----1-41-11----1---------
1544MarkhamAdenylosuccinate synthetase fr ...Azotobacter vinelandiiArch. Biochem. Biophys.18424-351977------15---1--2--11----11----11-------------15----1----1----11----11--------
1545MatsudaAdenylosuccinate synthetase in ...Rattus norvegicusBiochem. Biophys. Res. Commun.78766-7711977------27-----2-----2--1--------------------2-7---------2--1---------------
1546OgawaPurification, crystallization, ...Rattus norvegicusJ. Biochem.81859-8691977---1--73-41--2--1--21-11----11---------1---7-3-41----1-21-11----11--------
1547SpectorMammalian adenylosuccinate syn ...Oryctolagus cuniculusBiochim. Biophys. Acta445509-5171976------113-----2-----1--4--------------------11-3---------1--4---------------
1548Van der WeydenHuman adenylosuccinate synthet ...Homo sapiensJ. Biol. Chem.2497282-72891974------213-6---2--11-21-1-----1--------------21-3-6-----1-21-1-----1---------
1549MuirheadPurification of adenylosuccina ...Oryctolagus cuniculusJ. Biol. Chem.249459-4641974---1-182-----2--1--31-1-----1----------1-1-8-2-------1-31-1-----1---------
1550NagyRegulation of the biosynthesis ...Schizosaccharomyces pombeBiochim. Biophys. Acta3091-101973------2------2--11----1--------------------2---------1----1---------------