Purification and structural characterization of rat liver threonyl transfer ribonucleic acid synthetase

Dignam, J.D.; Rhodes, D.G.; Deutscher, M.P.; Biochemistry 19, 4978-4984 (1980)

 show all sequences of 6.1.1.3

Data extracted from this reference:

KM Value [mM]
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
0.012
-
 tRNAThr
-
 Rattus norvegicus
0.4
-
 2-Chloroadenosine 5'-triphosphate ATP Rattus norvegicus
0.5
-
 Formycin 5'-triphosphate threonine Rattus norvegicus
Molecular Weight [Da]
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
154000
-
 sedimentation equilibrium measurement Rattus norvegicus
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Rattus norvegicus
-
-
-
Posttranslational Modification
Posttranslational Modification Commentary Organism
glycoprotein
-
 Rattus norvegicus
Purification (Commentary)
Commentary Organism
-
 Rattus norvegicus
Source Tissue
Source Tissue Commentary Organism Textmining
liver
-
 Rattus norvegicus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Commentary Organism
0.7
-
-
 Rattus norvegicus
Storage Stability
Storage Stability Organism
-80°C, about 30% loss of activity Rattus norvegicus
Substrates and Products (Substrate)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
2-Chloroadenosine 5'-triphosphate + L-threonine + tRNAThr
-
 411 Rattus norvegicus ?
-
-
-
-
ATP + L-threonine + tRNAThr
-
 411 Rattus norvegicus AMP + diphosphate + L-threonyl-tRNAThr
-
 411 Rattus norvegicus
-
Formycin 5'-triphosphate + L-threonine + tRNAThr
-
 411 Rattus norvegicus ?
-
-
-
-
Subunits
Subunits Commentary Organism
dimer 2 * 85000, SDS-PAGE Rattus norvegicus
KM Value [mM] (protein specific)
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
0.012
-
 tRNAThr
-
 Rattus norvegicus
0.4
-
 2-Chloroadenosine 5'-triphosphate ATP Rattus norvegicus
0.5
-
 Formycin 5'-triphosphate threonine Rattus norvegicus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
154000
-
 sedimentation equilibrium measurement Rattus norvegicus
Posttranslational Modification (protein specific)
Posttranslational Modification Commentary Organism
glycoprotein
-
 Rattus norvegicus
Purification (Commentary) (protein specific)
Commentary Organism
-
 Rattus norvegicus
Source Tissue (protein specific)
Source Tissue Commentary Organism Textmining
liver
-
 Rattus norvegicus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Commentary Organism
0.7
-
-
 Rattus norvegicus
Storage Stability (protein specific)
Storage Stability Organism
-80°C, about 30% loss of activity Rattus norvegicus
Substrates and Products (Substrate) (protein specific)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
2-Chloroadenosine 5'-triphosphate + L-threonine + tRNAThr
-
 411 Rattus norvegicus ?
-
-
-
-
ATP + L-threonine + tRNAThr
-
 411 Rattus norvegicus AMP + diphosphate + L-threonyl-tRNAThr
-
 411 Rattus norvegicus
-
Formycin 5'-triphosphate + L-threonine + tRNAThr
-
 411 Rattus norvegicus ?
-
-
-
-
Subunits (protein specific)
Subunits Commentary Organism
dimer 2 * 85000, SDS-PAGE Rattus norvegicus

See also following references to EC number 6.1.1.3 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
714230MinajigiFidelity escape by the unnatur ...Escherichia coliBiochemistry501101-110920111-1----1-1-1-3--1-----3-1---1--1---1-11------1-1-1---1----3-1---1---------
716387SuAn unusual tRNAThr derived fro ...Candida albicans, Saccharomyces cerevisiaeNucleic Acids Res.394866-48742011-------4-25-2-5--------4-25--32--2------2------4-25-2--------4-25--32----44-33
715520MinajigiAminoacyl transfer rate dictat ...Escherichia coliJ. Biol. Chem.28523810-238172010----2----1-1-3--------211--11--1------1-2------1-1--------211--11----11---
716771LingSevere oxidative stress induce ...Escherichia coli, Escherichia coli MG1655Proc. Natl. Acad. Sci. USA1074028-40332010------22-1-1-3--------1-1--11--1------1----2-2-1-1--------1-1--11----11-11
704188MaldeBinding and enantiomeric selec ...Pyrococcus abyssiJ. Am. Chem. Soc.1313848-38492009-----------1-1--------1--------1------1----------1--------1---------------
705167ShimizuTwo complementary enzymes for ...Aeropyrum pernixJ. Mol. Biol.394286-2962009--11-----1-2-6--1-----21-------1-----221-------2-2---2----21---------12---
705818BerglundDual targeting to mitochondria ...Arabidopsis thalianaMol. Plant21298-13092009--1-----2-11-3--1-----1--------1-----11-------2-11---1----1----------11---
690277ShimizuCrystallization and preliminar ...Aeropyrum pernix, Sulfolobus tokodaiiActa Crystallogr. Sect. F64903-9102008--44------6--7---------1-------------4-4--------6----------1--------------
674339CailletMutations in residues involved ...Escherichia coli K-12J. Bacteriol.1896839-68482007--1-32----11-1-3--10-----2-----1--10-----110-32------11-1---10----2-----1---------
673326HussainPost-transfer editing mechanis ...Pyrococcus abyssiEMBO J.254152-41622006--117----1-1-3--1-1---3-1---1--1-----1117------1-1---11---3-1---1---------
662443RuanA unique hydrophobic cluster n ...Archaeoglobus fulgidus, Escherichia coli, Halobacterium sp., Helicobacter pylori, Methanocaldococcus jannaschii, Sulfolobus solfataricusJ. Biol. Chem.280571-5772005--6-12-710-7-7-13--6-----7-7--86--5218----652-12--71810-7-7---6----7-7--86---------
660587DwivediCloning, expression, purificat ...Pyrococcus abyssiActa Crystallogr. Sect. D601662-16642004--11-------1-2--1-----2--------2-----121---------1---1----2---------------
662829Dock-BregeonAchieving error-free translati ...Escherichia coliMol. Cell16375-3862004--11--1--2-2-2--1-----3-1---1--3-----131---1---2-2---1----3-1---1---------
663210KorencicA freestanding proofreading do ...Methanocaldococcus jannaschii, Sulfolobus solfataricusProc. Natl. Acad. Sci. USA10110260-102652004--2-2--6-2-4-4--------822--42--4-----24-2----6-2-4--------822--42---------
663379RigdenArchaea recruited D-Tyr-tRNATy ...Methanosarcina mazeiRNA101845-18512004--2--------2-2---2----32-------4-----24----------2--------32--------------
663398Zurek-MM and QM/MM modeling of threo ...Escherichia coliStruct. Chem.15405-4142004---1-----2-1-1---1----2--------2------21-------2-1--------2---------------
650200BoveeInduced fit and kinetic mechan ...Escherichia coliBiochemistry4215102-151132003--1-1--5-1-1-3--11----1----4---1-----11-1----5-1-1---1----1----4----------
652919Torres-LariosConformational movements and c ...Staphylococcus aureusJ. Mol. Biol.331201-2112003--11-----1-1-3--11----2-----1--1-----111-------1-1---1----2-----1---------
653273CailletThe modular structure of Esche ...Escherichia coliMol. Microbiol.47961-9742003--1-19--22--2-3---1----4----3---1-----11-19----22--2--------4----3----------
654016Nagaoka-Evolution and tRNA recognition ...Aeropyrum pernix, Haloferax volcaniiViva Orig.3162-702003--1------1-2-3--56----416---2--6-----16--------1-2---5----416---2---------
653324Torres-LariosStructural basis of translatio ...Escherichia coliNat. Struct. Biol.9343-3472002---11-12---1-3---1----3--------1------111--1-2---1--------3---------------
650778Dock-BregeonTransfer RNA-mediated editing ...Escherichia coliCell103877-8842000---13----1-1-2---1----2--------1------113------1-1--------2---------------
652046NathansonActive aminoacyl-tRNA syntheta ...Cricetulus griseus, Oryctolagus cuniculusJ. Biol. Chem.27531559-315622000--------4--4-2---4-24-6-2---2--4------4-------4--4-----24-6-2---2---------
653312SankaranarayananZinc ion mediated amino acid d ...Escherichia coliNat. Struct. Biol.7461-4652000---11--6-1-1-2---1----4-1--61--1------111----6-1-1--------4-1--61---------
650784SankaranarayananThe structure of threonyl-tRNA ...Escherichia coliCell97371-3811999---1-----1-1-2---1----1--------1------11-------1-1--------1---------------
395FreistThreonyl-tRNA synthetase. ...Aesculus hippocastanum, Bos taurus, Escherichia coli, Geobacillus stearothermophilus, Homo sapiens, Mus musculus, Oryctolagus cuniculus, Rattus norvegicus, Saccharomyces carlsbergensis, Saccharomyces cerevisiae, Thermus thermophilusBiol. Chem. Hoppe-Seyler376213-2241995--1---1-117---12-----6--11--------------1-----1--117-------6--11---------------
398CuraCrystallization of threonyl-tR ...Thermus thermophilusFEBS Lett.374110-1121995--11------11-4--1-----2--------------1-1--------11---1----2---------------
402NamekiIdentity elements of tRNAThr t ...Saccharomyces cerevisiaeNucleic Acids Res.232831-28361995-------1-----4--------1----------------------1------------1---------------
396OgataPresence and role of the 5SrRN ...Rattus norvegicusBiochim. Biophys. Acta1218388-4001994--------1----1--1--11-1-----------------------1------1-11-1---------------
397ZheltonosovaThreonyl-tRNA synthetase from ...Escherichia coli, Thermus thermophilusBiochimie7671-771994-------4--2--5--1---112222--4-----------------4--2----1--112222--4----------
399FreistThreonyl-tRNA synthetase from ...Saccharomyces cerevisiaeEur. J. Biochem.220745-7521994-------1-----3--1-----3----1-----------------1-------1----3----1----------
400CruzenNucleotide and deduced amino a ...Homo sapiensJ. Biol. Chem.2669919-99231991--1-------1--5--------1--------------1----------1---------1---------------
401GarberCrystals of threonyl-tRNA synt ...Thermus thermophilusJ. Mol. Biol.214819-8201990---1---------2--------1----------------1------------------1---------------
403AoyamaSpermine stimulates the threon ...Rattus norvegicusChem. Biol. Interact.7433-4319901------------3-----2--1------------1-------------------2--1---------------
415FedorovInteraction of eukaryotic thre ...Oryctolagus cuniculusEur. J. Biochem.169185-1911987-------------3--1--2-121-----------------------------1-2-121--------------
414PapeCloning and characterization o ...Saccharomyces cerevisiae, Saccharomyces cerevisiae D273-10BNucleic Acids Res.136171-61831985--1-----3----4--------1--------------1--------3-----------1---------------
413GerkenThreonyl-tRNA synthetase from ...Cricetulus griseusJ. Biol. Chem.25911160-111611984----1----3---3-----5--1-----------------1------3-------5--1---------------
92WalkerMolecular weights of mitochond ...Bos taurusBiochemistry221934-19411983--------2-2--1-----1--1-----------------------2-2------1--1---------------
411DignamPurification and structural ch ...Rattus norvegicusBiochemistry194978-49841980-------3--1--3-11--21131---------------------3--1---11-21131--------------
169ArbeenyThe activity and sedimentation ...Rattus norvegicusBiochim. Biophys. Acta564191-2011979--------1----1-----2--1-----------------------1--------2--1---------------
273FreistThreonyl-tRNA, lysyl-tRNA and ...Saccharomyces cerevisiaeEur. J. Biochem.84499-5021978------62-----1--------3--------------------6-2------------3---------------
409YamadaRapid purification of threonyl ...Saccharomyces carlsbergensisJ. Biochem.831583-15891978-------3-126--4--6---6-16---6-----------------3-126----6--6-16---6----------
407HenneckeThreonyl-transfer ribonucleic ...Escherichia coli, Escherichia coli overproducingJ. Bacteriol.131943-9501977----------2--3--1-----11------------------------2----1----11--------------
405AoyamaEffect of spermine on the reac ...Rattus norvegicusBiochim. Biophys. Acta309502-50719731------------2-----2--1------------1-------------------2--1---------------
406PaetzBiochemical and immunological ...Escherichia coliEur. J. Biochem.35331-3371973----1--------2--1----11---2-1-----------1------------1---11---2-1---------
404HolleyPartial purification of the th ...Rattus norvegicusJ. Biol. Chem.236197-1991961---------3---1--1--1--1------------------------3-----1-1--1---------------