Active aminoacyl-tRNA synthetases are present in nuclei as a high molecular weight multienzyme complex

Nathanson, L.; Deutscher, M.P.; J. Biol. Chem. 275, 31559-31562 (2000)

 show all sequences of 6.1.1.15

Data extracted from this reference:

Localization
Localization Commentary Organism GeneOntology No. Textmining
cytoplasm major part, enzyme participates in a multienzyme complex, not as large and stable as the one from nucleus Cricetulus griseus 5737
-
nucleus 2.9% of total activity in the cell, enzyme participates in a large and stable multienzyme complex Cricetulus griseus 5634
-
Natural Substrates/ Products (Substrates)
Natural Substrates Organism Commentary (Nat. Sub.) Natural Products Commentary (Nat. Pro.) Organism (Nat. Pro.) Reversibility
ATP + L-proline + tRNAPro Cricetulus griseus the reaction catalyzed by the enzyme plays an important role in the transport of aminoacylated tRNAs from the nucleus to the cytoplasm AMP + diphosphate + L-prolyl-tRNAPro
-
 Cricetulus griseus ?
More Cricetulus griseus aminoacyl-tRNA is channeled in vivo by probably direct transfer to elongation factor I ?
-
 Cricetulus griseus ?
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Cricetulus griseus
-
-
-
Reaction
Reaction Commentary Organism
ATP + L-proline + tRNAPro = AMP + diphosphate + L-prolyl-tRNAPro
-
 Cricetulus griseus
Source Tissue
Source Tissue Commentary Organism Textmining
ovary cell line CRL-1781 Cricetulus griseus
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Commentary Organism
additional information
-
-
 Cricetulus griseus
Substrates and Products (Substrate)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
ATP + L-proline + tRNAPro
-
 652046 Cricetulus griseus AMP + diphosphate + L-prolyl-tRNAPro
-
 652046 Cricetulus griseus ?
ATP + L-proline + tRNAPro the reaction catalyzed by the enzyme plays an important role in the transport of aminoacylated tRNAs from the nucleus to the cytoplasm 652046 Cricetulus griseus AMP + diphosphate + L-prolyl-tRNAPro
-
 652046 Cricetulus griseus ?
More aminoacyl-tRNA is channeled in vivo by probably direct transfer to elongation factor I 652046 Cricetulus griseus ?
-
 652046 Cricetulus griseus ?
Temperature Optimum [°C]
Temperature Optimum [°C] Temperature Optimum Maximum [°C] Commentary Organism
37
-
 assay at Cricetulus griseus
pH Optimum
pH Optimum Minimum pH Optimum Maximum Commentary Organism
7.5
-
 assay at Cricetulus griseus
Cofactor
Cofactor Commentary Organism Structure
ATP
-
 Cricetulus griseus
Cofactor (protein specific)
Cofactor Commentary Organism Structure
ATP
-
 Cricetulus griseus
Localization (protein specific)
Localization Commentary Organism GeneOntology No. Textmining
cytoplasm major part, enzyme participates in a multienzyme complex, not as large and stable as the one from nucleus Cricetulus griseus 5737
-
nucleus 2.9% of total activity in the cell, enzyme participates in a large and stable multienzyme complex Cricetulus griseus 5634
-
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates Organism Commentary (Nat. Sub.) Natural Products Commentary (Nat. Pro.) Organism (Nat. Pro.) Reversibility
ATP + L-proline + tRNAPro Cricetulus griseus the reaction catalyzed by the enzyme plays an important role in the transport of aminoacylated tRNAs from the nucleus to the cytoplasm AMP + diphosphate + L-prolyl-tRNAPro
-
 Cricetulus griseus ?
More Cricetulus griseus aminoacyl-tRNA is channeled in vivo by probably direct transfer to elongation factor I ?
-
 Cricetulus griseus ?
Source Tissue (protein specific)
Source Tissue Commentary Organism Textmining
ovary cell line CRL-1781 Cricetulus griseus
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Commentary Organism
additional information
-
-
 Cricetulus griseus
Substrates and Products (Substrate) (protein specific)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
ATP + L-proline + tRNAPro
-
 652046 Cricetulus griseus AMP + diphosphate + L-prolyl-tRNAPro
-
 652046 Cricetulus griseus ?
ATP + L-proline + tRNAPro the reaction catalyzed by the enzyme plays an important role in the transport of aminoacylated tRNAs from the nucleus to the cytoplasm 652046 Cricetulus griseus AMP + diphosphate + L-prolyl-tRNAPro
-
 652046 Cricetulus griseus ?
More aminoacyl-tRNA is channeled in vivo by probably direct transfer to elongation factor I 652046 Cricetulus griseus ?
-
 652046 Cricetulus griseus ?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C] Temperature Optimum Maximum [°C] Commentary Organism
37
-
 assay at Cricetulus griseus
pH Optimum (protein specific)
pH Optimum Minimum pH Optimum Maximum Commentary Organism
7.5
-
 assay at Cricetulus griseus

See also following references to EC number 6.1.1.15 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
691088BurkeFunctional guanine-arginine in ...Escherichia coliBiochim. Biophys. Acta17841222-12252008----4--1---1-3--------2-----------------4----1---1--------2---------------
693220SplanTransfer RNA modulates the edi ...Escherichia coli, Homo sapiens, Methanocaldococcus jannaschiiJ. Biol. Chem.2837128-71342008----1--4-3-3-7------1-7-3--33--3------3-1----4-3-3------1-7-3--33---------
693908WeiInability of HIV-1 produced in ...Moloney murine leukemia virusJ. Virol.8212049-120592008---------1-1-8--------2--------1------1--------1-1--------2---------------
694001KoehrerThe many applications of acid ...Methanocaldococcus jannaschii, Methanopyrus kandleri, Methanothermobacter thermautotrophicusMethods44129-1382008---------3-3-5--------9--------3------3--------3-3--------9---------------
694123JiaWHEP domains direct noncanonic ...Homo sapiensMol. Cell29679-6902008--1------112-2--------31-------1-----11--------112--------31--------------
694434AnEvolution of acceptor stem tRN ...Homo sapiensNucleic Acids Res.362514-25212008----1----1-1-4--------3-1------1------1-1------1-1--------3-1-------------
674875Praetorius-IbbaFunctional association between ...Methanothermobacter thermautotrophicusJ. Biol. Chem.2823680-368720071-1----1-1-1-4--1-----2-1---1--1---1-11------1-1-1---1----2-1---1---------
674646HatiPre-transfer editing by class ...Escherichia coli, Methanocaldococcus jannaschii, Methanococcus maripaludis, Methanothermobacter thermautotrophicus, Saccharomyces cerevisiaeJ. Biol. Chem.28127862-278722006--5-2-28-5-5-7------5-9-4--84--51----55-2--218-5-5------5-9-4--84---------
675395ZhangDistinct kinetic mechanisms of ...Deinococcus radioduransJ. Mol. Biol.361300-3112006-------1-1-1-1---1----2-1--31--1------1------1-1-1--------2-1--31---------
677155CrepinStructures of two bacterial pr ...Enterococcus faecalis, Rhodopseudomonas palustrisStructure141511-15252006--21--2--2-2-5--2-----62-------2-----221---2---2-2---2----62--------------
660617MurayamaStructure of a putative trans- ...Aeropyrum pernixActa Crystallogr. Sect. F6126-292005---1---------5-------------------------1----------------------------------
661767AmbrogellyAsymmetric behavior of archaea ...Methanocaldococcus jannaschii, Methanothermobacter thermautotrophicusFEBS Lett.5796017-60222005----1--2--2--4---2----22---2------------1----2--2---------22---2----------
662383Praetorius-IbbaAssociation between Archaeal p ...Methanothermobacter thermautotrophicusJ. Biol. Chem.28026099-261042005-------6-----3--------11---4-----------------6------------11---4----------
662414AnCys-tRNA(Pro) editing by Haemo ...Haemophilus influenzaeJ. Biol. Chem.28034465-344722005-------------3---------1-----------------------------------1--------------
661440SampathNoncanonical function of gluta ...Homo sapiensCell119195-2082004-----------1-2-1---1--11-------------------------1--1--1--11--------------
653591KamtekarThe structural basis of cystei ...Methanocaldococcus jannaschii, Methanothermobacter thermautotrophicusProc. Natl. Acad. Sci. USA1001673-16782003--12--24-2-2-6--22----4-2---2--22----122---224-2-2---2----4-2---2---------
650167WongFunctional role of the prokary ...Escherichia coliBiochemistry417108-71152002----8------1-3---1----4--------1------1-8--------1--------4---------------
651296Jacquin-BeckerCysteinyl-tRNA formation and p ...Giardia intestinalis, Methanocaldococcus jannaschii, Methanococcus maripaludis, Thermus thermophilusFEBS Lett.51434-3620021-113-1----6-5--14----12--------4---1-1413--1-----6---1----12---------------
652298AhelCysteine activation is an inhe ...Aquifex aeolicus, Borrelia burgdorferi, Clostridium sticklandii, Cytophaga hutchinsonii, Deinococcus radiodurans, Escherichia coli, Magnetospirillum magnetotacticum, Methanocaldococcus jannaschii, Methanothermobacter thermautotrophicus, Novosphingobium aromaticivorans, Rhodopseudomonas palustris, Saccharomyces cerevisiae, Thermus thermophilusJ. Biol. Chem.27734743-347482002-------26---13-27--1313----39-13--2613--13------13------26---13---13----39-13--2613---------
652299AmbrogellyMethanocaldococcus jannaschii ...Methanocaldococcus jannaschiiJ. Biol. Chem.27734749-347542002--1-1-6----1-4--11----4-1---2--1-----11-1--6-----1---1----4-1---2---------
650058StathopoulosMethanococcus jannaschii proly ...Methanocaldococcus jannaschiiBiochemistry4046-5220012---2-38---1-3--11----4-1--42--1---2--1-2--3-8---1---1----4-1--42---------
650558YuA series of quinoline analogue ...Candida albicans, Homo sapiensBioorg. Med. Chem. Lett.11541-5442001------50----2-2---2----2--------21-----2----501----2--------2---------------
652131BurkeDivergent adaptation of tRNA r ...Methanocaldococcus jannaschiiJ. Biol. Chem.27620286-202912001--1--------1-5--11----3-1------1-----11----------1---1----3-1-------------
652154BeuningSpecies-specific differences i ...Escherichia coli, Homo sapiens, Methanocaldococcus jannaschiiJ. Biol. Chem.27630779-307852001-2-----6---3-7---3----11-3--6---3----2-3------6---3--------11-3--6----------
652859YaremchukA succession of substrate indu ...Thermus thermophilusJ. Mol. Biol.309989-10022001---1-----1-1-3---1----2--------1------11-------1-1--------2---------------
649132YaremchukImproved crystals of Thermus t ...Thermus thermophilusActa Crystallogr. Sect. D56197-1992000---1-------1-2---1----1--------1------11---------1--------1---------------
650975YaremchukCrystal structure of a eukaryo ...Thermus thermophilusEMBO J.194745-47582000---1-----1-1-4---1----2--------1------11-------1-1--------2---------------
652046NathansonActive aminoacyl-tRNA syntheta ...Cricetulus griseusJ. Biol. Chem.27531559-315622000--------2--2-1---1-11-3-1---1--1------1-------2--2-----11-3-1---1---------
651917NorcumImmunoelectron microscopic loc ...Oryctolagus cuniculusJ. Biol. Chem.27412205-122081999-----------1-3---1----12-------1------1----------1--------12--------------
653918CusacktRNAPro anticodon recognition ...Thermus thermophilusStructure6101-1081998---1-------1-3---1----2--------1------11---------1--------2---------------
164StehlinChemical modification and site ...Escherichia coliBiochemistry362932-29381997----3-14-----3--------1----4------------3--1-4------------1----4----------
160Heacock-Synthesis and aminoacyl-tRNA s ...Escherichia coliBioorg. Chem.24273-2891996------1------1--------2--------------------1--------------2---------------
161KerjanMammalian prolyl-tRNA syntheta ...Homo sapiens, Mammalia, Ovis ariesBiochimie74195-2051992-------------7-----2--31-------------------------------2--31--------------
163TingIsolation of prolyl-tRNA synth ...Rattus norvegicusJ. Biol. Chem.26717701-177091992-------1--1--2--1--22-11---------------------1--1----1-22-11--------------
165BianchiPolypeptide composition of the ...Rattus norvegicusBiochem. Biophys. Res. Commun.1871071-10761992-------------2--1--21-1------------------------------1-21-1---------------
26OgataOccurence of 5SrRNA in high mo ...Rattus norvegicusJ. Biochem.1101030-10361991-------------3--1--11-11-----------------------------1-11-11--------------
126NorcumIsolation and electron microsc ...Mus musculusJ. Biol. Chem.26415043-150511989-------------1-----1---1-------------------------------1---1--------------
166RiveraA highly thermostable proline: ...Thermus aquaticusCan. J. Biochem. Cell Biol.62507-5151984----------1--1--1---1111-12-111-----------------1----1--1111-12-111-------
92WalkerMolecular weights of mitochond ...Bos taurusBiochemistry221934-19411983-------12-2--1--------1----------------------12-2---------1---------------
168Busiellobeta-Selenaproline as competit ...Escherichia coli, Rattus norvegicusBiochim. Biophys. Acta606347-3521980------26-----2-----1--6--------------------2-6---------1--6---------------
169ArbeenyThe activity and sedimentation ...Rattus norvegicusBiochim. Biophys. Acta564191-2011979--------1----1-----2--1-----------------------1--------2--1---------------
170JeanninAminoacylation of Phaseolus vu ...Escherichia coli, Phaseolus vulgarisBiochim. Biophys. Acta44224-311976--------3----5--------4-----------------------3-----------4---------------
171Norris-Cold-lability of prolyl-tRNA s ...Delonix regia, Phaseolus vulgarisPhytochemistry131677-16871974-----72------2-------22---4--------------7-2-------------22---4-----------
172Norris-Pro-tRNA synthetase from Phase ...Delonix regia, Phaseolus vulgarisPhytochemistry122829-28411973-----1-------2--------2------------------1----------------2---------------
173Norris-A Comparison of the thermal st ...Delonix regia, Phaseolus vulgarisPhytochemistry122109-21211973-----13-------2--------2---2--------------13----------------2---2-----------
174Norris-Substrate discrimination by pr ...Beta vulgaris, Convallaria majalis, Delonix regia, Hemerocallis fulva, Parkinsonia aculeata, Phaseolus vulgaris, Polygonatum multiflorum, Ranunculus bulbosusPhytochemistry112921-29351972-----74729-----8--2---2-27---12-8------------7-47-29-------2--2-27---12-8---------
175PapasKinetic studies of the prolyl ...Escherichia coliJ. Biol. Chem.2465924-59281971-------------1---1----1-----------------------------------1---------------
177PetersonPurification, properties and c ...Asparagus officinalis, Convallaria majalis, Phaseolus vulgaris, Pisum sativum, Polygonatum multiflorumBiochem. J.97112-1241965------1--4---13--2--5--7---1-2--------------1---4-----2-5--7---1-2---------
176NortonPurification and properties of ...Escherichia coliArch. Biochem. Biophys.106147-1521964------12-----3--1---1-2------1-------------1-2-------1--1-2------1--------