Tyrosyl-tRNA synthetase of Escherichia coli B. Role of magnesium in the reaction catalyzed by the enzyme

Chousterman, S.; Chapeville, F.; Eur. J. Biochem. 35, 46-50 (1988)

 show all sequences of 6.1.1.1

Data extracted from this reference:

Metals/Ions
Metals/Ions Commentary Organism Structure
Mg2+ required, spermidine can substitute for Mg2+ in the transacylation reaction, i.e. transfer of tyrosine from the tyrosine-AMP-enzyme complex to tRNA, but not in the tyrosine activation reaction, i.e. formation of the tyrosine-AMP-enzyme complex from tyrosine + ATP + enzyme Escherichia coli
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Escherichia coli
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-
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Metals/Ions (protein specific)
Metals/Ions Commentary Organism Structure
Mg2+ required, spermidine can substitute for Mg2+ in the transacylation reaction, i.e. transfer of tyrosine from the tyrosine-AMP-enzyme complex to tRNA, but not in the tyrosine activation reaction, i.e. formation of the tyrosine-AMP-enzyme complex from tyrosine + ATP + enzyme Escherichia coli

See also following references to EC number 6.1.1.1 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
714246PaukstelisNMR structure of the C-termina ...Emericella nidulansBiochemistry503816-38262011--1-1---1----6--1------2-------------1--1-----1------1-----2---------11---
714470Xiao4-Alkoxy-3-arylfuran-2(5H)-one ...Staphylococcus aureusBioorg. Med. Chem.193884-38912011------23------4--------------------22-------2223------------------------------
715585VoDissociating quaternary struct ...Homo sapiensJ. Biol. Chem.28611563-115682011-------------3-------------------------------------------------------22---
715934LarsonThe double-length tyrosyl-tRNA ...Leishmania majorJ. Mol. Biol.409159-1762011--1---2------4--------11-------------1-----2--------------11---------11---
702091SunRational design of aminoacyl-t ...Methanocaldococcus jannaschiiBiochem. Biophys. Res. Commun.391709-7152010---11------1-4--------2--------1------111--------1--------2---------------
705174ChadeeProtein roles in group I intro ...Neurospora crassaJ. Mol. Biol.395656-6702010--1-----3--1-4--1-----1--------1-----11-------3--1---1----1----------11---
705987IrahaFunctional replacement of the ...Escherichia coli, Methanocaldococcus jannaschii, Saccharomyces cerevisiaeNucleic Acids Res.383682-36912010-3312------3-6--1-----9--------3----33312--------3---1----9---------------
715923KuhnEngineering of an orthogonal a ...Methanocaldococcus jannaschiiJ. Mol. Biol.40470-872010----2--------3--------------------------2---------------------------------
702575MelanconOne plasmid selection system f ...Methanocaldococcus jannaschiiBioorg. Med. Chem. Lett.193845-38472009-1---------1-3--------2--------1----1-1----------1--------2---------------
703176KapoorMutational separation of amino ...Homo sapiensChem. Biol.16531-5392009-1116------1-3--1--2--1-1---1--1----11116--------1---1-2--1-1---1---------
704355KamijoAnalyses of conditions for KMS ...Methanocaldococcus jannaschiiJ. Biochem.146241-2502009--1-3--3---1-3--1-----1-1--41--1-----11-3----3---1---1----1-1--41---------
704609SharmaThermodynamic analysis reveals ...Geobacillus stearothermophilusJ. Biol. Chem.2844179-41902009--1------1-1-3--1-----1--1--1--1-----11--------1-1---1----1--1--1---------
705526KimHigh glucose inhibits gene exp ...Rattus norvegicusMethods Find. Exp. Clin. Pharmacol.31639-6442009-11--------1-1-----2--1--------1----111----------1-----2--1---------1--1--
706503StorkebaumDominant mutations in the tyro ...Drosophila melanogaster, Homo sapiens, Saccharomyces cerevisiaeProc. Natl. Acad. Sci. USA10611782-117872009-23-6---3--3-6--1--2--3--------3----233-6-----3--3---1-2--3---------------
690373ChengEffect of mini-tyrosyl-tRNA sy ...Mus musculusAm. J. Physiol. Regul. Integr. Comp. Physiol.295R1138-R11462008-1--1--------3-----4----------------1---1--------------4------------------
691422GoerkeHigh-level cell-free synthesis ...Methanocaldococcus jannaschiiBiotechnol. Bioeng.102400-4162008-11----------2----------------------11------------------------------------
692144LiComparative structural dynamic ...Staphylococcus aureusEur. Biophys. J.3825-352008-------------2---1--------------------------------------------------------
692266GreenbergThe novel fragment of tyrosyl ...Bos taurusFASEB J.221597-16052008-1--3-----1--3-----2----------------1---3-------1------2------------------
693106HauensteinThe homotetrameric phosphosery ...Methanosarcina mazeiJ. Biol. Chem.28321997-220062008-------------4------------------------------------------------------------
694339PaukstelisStructure of a tyrosyl-tRNA sy ...Neurospora crassaNature45194-972008---1---------4-------------------------1----------------------------------
694895PaukstelisIdentification and evolution o ...Ajellomyces capsulatus, Coccidioides posadasii, Emericella nidulansProc. Natl. Acad. Sci. USA1056010-60152008--3-----43---9--3---3-3-3---3--------3--------43-----3--3-3-3---3---------
695107ParkOverexpressed mitochondrial le ...Methanocaldococcus jannaschiiRNA142407-24162008-1-----------1----------------------1-------------------------------------
695113BonnefondDecreased aminoacylation in pa ...Homo sapiensRNA14641-6482008-------41----3------1-1-1--41----------------41---------1-1-1--41---------
675706AbergelVirus-encoded aminoacyl-tRNA s ...Acanthamoeba polyphaga MimivirusJ. Virol.8112406-124172007--11---1-1-2-3------1-311---1--1-----111-----1-1-2------1-311---1---------
676225TsunodaStructural basis for recogniti ...Saccharomyces cerevisiaeNucleic Acids Res.354289-43002007--111-1--1-1-3--11----31-------1-----1111--1---1-1---1----31--------------
677165BonnefondCrystal structure of human mit ...Homo sapiensStructure151505-15162007--113---21-1-4--11----11-------1-----1113-----21-1---1----11--------------
662662KurataniCrystal structures of tyrosyl- ...Aeropyrum pernix, Archaeoglobus fulgidus, Pyrococcus horikoshiiJ. Mol. Biol.355395-4082006---3---------7--3----------------------3-------------3--------------------
675162KordyshConformational flexibility of ...Bos taurusJ. Fluoresc.16705-7112006--1----------1--1-----11-------------1---------------1----11--------------
676126JordanovaDisrupted function and axonal ...Homo sapiensNat. Genet.38197-2022006--1-3---21-2-3-----6--2-1---1--1-----11-3-----21-2-----6--2-1---1---------
676862TurnerStructural plasticity of an am ...Methanocaldococcus jannaschiiProc. Natl. Acad. Sci. USA1036483-64882006--111----1-4-1--1-----8--------1-----1111------1-4---1----8---------------
658121BonnefondToward the full set of human m ...Homo sapiensBiochemistry444805-48162005--1----22-1--4--1-----11---1---------1-------22-1----1----11---1----------
660625AbergelMimivirus TyrRS: preliminary s ...MimivirusActa Crystallogr. Sect. FF61212-2152005---1---------4--1----------------1-----1-------------1-------------1------
662639KobayashiStructural snapshots of the KM ...Escherichia coliJ. Mol. Biol.346105-1172005--11---------3--1--------------------1-1-------------1--------------------
662832PaukstelisA tyrosyl-tRNA synthetase adap ...Neurospora crassaMol. Cell17417-4282005---1----2--1-2--1-----1----------------1------2--1---1----1---------------
663338ZhangCrystal structures of apo wild ...Methanocaldococcus jannaschiiProtein Sci.141340-13492005---1---------3--1----------------------1-------------1--------------------
653754JiaExpression, purification, and ...Homo sapiensProtein Expr. Purif.27104-1082003--1----3-1-1-4--11-11-211--3---1-----11------3-1-1---1-11-211--3----------
650116EwaltActivation of angiogenic signa ...Homo sapiensBiochemistry4113344-133492002--------1--2-1-1-1----2--------1------1-------1--2--1-----2---------------
650993YaremchukClass I tyrosyl-tRNA synthetas ...Thermus thermophilusEMBO J.213829-38402002---1-----1-1-3---1----21-------1------11-------1-1--------21--------------
652238AustinCatalysis of tyrosyl-adenylate ...Homo sapiensJ. Biol. Chem.27714812-148202002-11----1-2-1-4--11----1-1---1--1----111------1-2-1---1----1-1---1---------
652281AustinComparison of the catalytic ro ...Geobacillus stearothermophilus, Homo sapiensJ. Biol. Chem.27728394-283992002--1-6--12-3-2-5--12----4-1--61--2-----12-6----12-3-2---1----4-1--61---------
653689ZhangStructure-based design of muta ...Methanocaldococcus jannaschiiProc. Natl. Acad. Sci. USA996579-65842002-1--2------1-3---1----2-------------1---2--------1--------2---------------
653691KigaAn engineered Escherichia coli ...Escherichia coliProc. Natl. Acad. Sci. USA999715-97202002-11-48--3-1-1-5--11--1-2-1--31--1----111-48----3-1-1---1--1-2-1--31---------
651278SalazarConserved amino acids near the ...Acidithiobacillus ferrooxidansFEBS Lett.491257-2602001--1-5--6-111-4--11--1-11---71--1-----11-5----6-111---1--1-11---71---------
651786OhnoChanging the amino acid specif ...Saccharomyces cerevisiaeJ. Biochem.130417-4232001-11-1--12-1-1-3--11----18-1--161--1----111-1----12-1-1---1----18-1--161---------
653786QiuCrystal structure of Staphyloc ...Staphylococcus aureusProtein Sci.102008-20162001-1-1--1----1-4---1----1--------1----1-11---1-----1--------1---------------
649953FechterIdentity of tRNA for yeast tyr ...Saccharomyces cerevisiaeBiochemistry391725-17332000----1--2---1-5---1----3----2---1------1-1----2---1--------3----2----------
652065Hamano-TakakuA mutant Escherichia coli tyro ...Escherichia coliJ. Biol. Chem.27540324-403282000--1-1--4-1-2-3--11----311-151--1-----11-1----4-1-2---1----311-151---------
652832XinStabilization of the transitio ...Geobacillus stearothermophilusJ. Mol. Biol.303299-3102000--1-5--1-1-1-3---1----2-1---1--1-----11-5----1-1-1--------2-1---1---------
650300AirasChloride affects the interacti ...Escherichia coli, Geobacillus stearothermophilusBiochim. Biophys. Acta147251-611999------96-2-2-5--12--1-3-2---2--114-----1----9146-2-2---1--1-3-2---2---------
650538BrownMolecular recognition of tyros ...Geobacillus stearothermophilus, Staphylococcus aureusBioorg. Med. Chem.72473-24851999-2-2--16--2-2-4--12----4-2---2--214---2-22---1614--2-2---1----4-2---2---------
650572JarvestInteraction of tyrosyl aryl di ...Staphylococcus aureusBioorg. Med. Chem. Lett.92859-28621999---1--1----1-1---1----1---------1------1---11----1--------1---------------
651887ParkDimeric tyrosyl-tRNA synthetas ...Geobacillus stearothermophilusJ. Biol. Chem.27318052-180591998--1-5--7---1-3--11-2--1---28---1-----11-5----7---1---1-2--1---28----------
19FirstAnalysis of the role of the KM ...Geobacillus stearothermophilusBiochemistry345030-50431995-------1-----2-------------------------------1----------------------------
12SalazarThiobacillus ferrooxydans tyro ...Acidithiobacillus ferrooxidansJ. Bacteriol.1764409-44151994--1-------1--5------1----------------1----------1-------1-----------------
13AvisReaction of modified and unmod ...Geobacillus stearothermophilusBiochemistry325312-53201993-------1-----4-------------------------------1----------------------------
14FirstInvolvement of threonine 234 i ...Geobacillus stearothermophilusBiochemistry3213644-136501993-------1-----4-------------------------------1----------------------------
15FirstMutation of lysine 233 to alan ...Geobacillus stearothermophilusBiochemistry3213651-136571993-------1-----3-------------------------------1----------------------------
16FirstMutational and kinetic analysi ...Geobacillus stearothermophilusBiochemistry3213658-136631993-------1-----2-------------------------------1----------------------------
24De PratModification of amino acid spe ...Geobacillus stearothermophilusFEBS Lett.318167-1711993----1--------3--------------------------1---------------------------------
17WellsKinetic and thermodynamic prop ...Geobacillus stearothermophilusBiochemistry305151-51561991----1--1-----3--------------------------1----1----------------------------
18FothergillCorrelations between kinetics ...Geobacillus stearothermophilusBiochemistry305157-51641991----2--1-----2--------------------------2----1----------------------------
21GarciaMetal ion dependence of phosph ...Geobacillus stearothermophilusBiochemistry291643-16481990-------------3--------1-----------------------------------1---------------
6WardAsymmetry of tyrosyl-tRNA synt ...Geobacillus stearothermophilusBiochemistry271041-10491988-------------3---------1-----------------------------------1--------------
7WardTyrosyl-tRNA synthetase acts a ...Geobacillus stearothermophilusBiochemistry275525-55301988-------------3---------1-----------------------------------1--------------
8Chousterman-Tyrosyl-tRNA synthetase of Esc ...Escherichia coliEur. J. Biochem.3546-501988---------1---1---------------------------------1--------------------------
20Brick-Structure of tyrosyl-tRNA synt ...Saccharomyces cerevisiaeJ. Mol. Biol.20883-981988---1---------1-------------------------1----------------------------------
22FreistTyrosyl-tRNA synthetase from b ...Saccharomyces cerevisiaeEur. J. Biochem.177425-4331988-------------4------1-1---------------------------------1-1---------------
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4JonesReversible dissociation of dim ...Geobacillus stearothermophilusBiochemistry245852-58571985---1---------3-------------------------1----------------------------------
5Brown-Crystal structure of two facti ...Geobacillus stearothermophilusBiochem. Soc. Trans.141228-12291985-------------1------------------------------------------------------------
3BhatTyrosyl-tRNA synthetase forms ...Geobacillus stearothermophilusJ. Mol. Biol.158699-7091982---1---------1-------------------------1----------------------------------
2GrosseThe binding of tyrosinyl-5'-AM ...Escherichia coliNucleic Acids Res.61631-16381979-------------2------------------------------------------------------------
1DeakPurification and some properti ...Rattus norvegicusBiochim. Biophys. Acta526626-6341978-------41----2-----11-2-----21---------------41--------11-2-----21--------
52Locy-Purification and characterizat ...Glycine maxPhytochemistry1719-271978-------62----1--1--1--1----------------------62------1-1--1---------------
53MonteilhetBinding of tyrosine, adenosine ...Geobacillus stearothermophilusJ. Mol. Biol.122407-4171978-------------1------------------------------------------------------------
50FaulhammerTyrosyl-tRNA synthetase from b ...Saccharomyces cerevisiaeEur. J. Biochem.75561-5701977------2---1--3--1---1-11-------------------2----1----1--1-11--------------
51Prasada RaoPurification and properies of ...Rattus norvegicusNucleic Acids Res.43887-39001977----------1--2-----11--1------------------------1------11--1--------------
47WarnerMechanism of suppression in Dr ...Drosophila melanogasterCan. J. Biochem.54650-6561976------13-21--5--1----11--------------------1-3-21----1---11---------------
49IrwinThe crystal structure of tyros ...Geobacillus stearothermophilusJ. Mol. Biol.105577-5861976-------------1------------------------------------------------------------
469FreistValyl-tRNA, isoleucyl-tRNA and ...Saccharomyces cerevisiaeEur. J. Biochem.66493-4971976------1------3--------1--------------------1--------------1---------------
45JakesTyrosyl-tRNA synthetase from E ...Escherichia coliBiochemistry143344-33501975-------1-----4--------1----------------------1------------1---------------
46BrutonGram-scale purification of met ...Escherichia coliEur. J. Biochem.59327-3331975-------------3------11----------------------------------11----------------
9Beikirch-Tyrosyl-tRNA synthetase ...Saccharomyces cerevisiaeMethods Enzymol.34B503-5061974--------1----1------1-------------------------1---------1-----------------
11Bhanot-Purification of tyrosine-tRNA ...Saccharomyces cerevisiaeMethods Enzymol.29 E547-5761974-------------1------1-----------------------------------1-----------------
44KochTyrosyl transfer ribonucleic a ...Geobacillus stearothermophilusBiochemistry132307-23121974----------1--1--1-----21------------------------1----1----21--------------
10ReidCrystallization and preliminar ...Geobacillus stearothermophilusJ. Mol. Biol.80199-2011973-------------2------------------------------------------------------------
42KucanPurification of tyrosine: tRNA ...Saccharomyces cerevisiaeJ. Biochem.73811-8191973-------11----1-------------1-----------------11----------------1----------
43ChoustermanTyrosyl-tRNA synthetase of Esc ...Escherichia coliEur. J. Biochem.3551-561973----------1--2--1----121------------------------1----1---121--------------
39BeikirchTyrosyl-tRNA synthetase from b ...Saccharomyces cerevisiaeEur. J. Biochem.26182-1901972--------121--2--1-----211---1-----------------121----1----211---1---------
40Buonocore-Properties of tyrosyl transfer ...Escherichia coliJ. Biol. Chem.247843-48491972-----1161-1--1-------12---1--------------1-1-61-1--------12---1-----------
41CowlesDemonstration of two tyrosyl-t ...Pisum sativumBiochim. Biophys. Acta28133-441972--------1-2--1--1--11-1-----------------------1-2----1-11-1---------------
38ChoustermanEscherichia coli tyrosyl-tRNA ...Escherichia coliFEBS Lett.17153-1571971---------1---2---------------------------------1--------------------------
37AllenIncorporation of amino acids i ...Cavia porcellusJ. Biol. Chem.2351061-10671960-----11------1--1--1-1------1------------1-1---------1-1-1------1---------