Chalcone isomerase from Citrus sinensis: purification and characterization

Fouche, S.D.; Dubery, I.A.; Phytochemistry 37, 127-132 (1994)

 show all sequences of 5.5.1.6

Data extracted from this reference:

Inhibitors
Inhibitors Commentary Organism Structure
5,7,4',5'-Tetrahydroxyflavanol
-
 Citrus sinensis
5,7,4'-Trihydroxyflavanone competitive towards 4,2',4',6'-tetrahydroxychalcone Citrus sinensis
morin competitive towards 4,2',4',6'-tetrahydroxychalcone Citrus sinensis
KM Value [mM]
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
0.004
-
 liquiritigenin 4,2',4',6'-tetrahydroxychalcone Citrus sinensis
Molecular Weight [Da]
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
27200
-
 gel filtration Citrus sinensis
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Citrus sinensis
-
-
-
Purification (Commentary)
Commentary Organism
-
 Citrus sinensis
Source Tissue
Source Tissue Commentary Organism Textmining
fruit developing Citrus sinensis
-
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Commentary Organism
additional information
-
-
 Citrus sinensis
Substrates and Products (Substrate)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
4,2',4',6'-Tetrahydroxychalcone i.e. naringenin chalcone 3735 Citrus sinensis 5,7,4'-Trihydroxyflavanone
-
 3735 Citrus sinensis
-
Subunits
Subunits Commentary Organism
monomer 1 * 27800, SDS-PAGE Citrus sinensis
Turnover Number [1/s]
Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Commentary Organism Structure
1.87
-
 4,2',4',6'-Tetrahydroxychalcone
-
 Citrus sinensis
pH Optimum
pH Optimum Minimum pH Optimum Maximum Commentary Organism
7.4
-
-
 Citrus sinensis
Inhibitors (protein specific)
Inhibitors Commentary Organism Structure
5,7,4',5'-Tetrahydroxyflavanol
-
 Citrus sinensis
5,7,4'-Trihydroxyflavanone competitive towards 4,2',4',6'-tetrahydroxychalcone Citrus sinensis
morin competitive towards 4,2',4',6'-tetrahydroxychalcone Citrus sinensis
KM Value [mM] (protein specific)
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
0.004
-
 liquiritigenin 4,2',4',6'-tetrahydroxychalcone Citrus sinensis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
27200
-
 gel filtration Citrus sinensis
Purification (Commentary) (protein specific)
Commentary Organism
-
 Citrus sinensis
Source Tissue (protein specific)
Source Tissue Commentary Organism Textmining
fruit developing Citrus sinensis
-
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Commentary Organism
additional information
-
-
 Citrus sinensis
Substrates and Products (Substrate) (protein specific)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
4,2',4',6'-Tetrahydroxychalcone i.e. naringenin chalcone 3735 Citrus sinensis 5,7,4'-Trihydroxyflavanone
-
 3735 Citrus sinensis
-
Subunits (protein specific)
Subunits Commentary Organism
monomer 1 * 27800, SDS-PAGE Citrus sinensis
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Commentary Organism Structure
1.87
-
 4,2',4',6'-Tetrahydroxychalcone
-
 Citrus sinensis
pH Optimum (protein specific)
pH Optimum Minimum pH Optimum Maximum Commentary Organism
7.4
-
-
 Citrus sinensis

See also following references to EC number 5.5.1.6 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
715092ParkEnhancement of flavone levels ...Scutellaria baicalensisFunct. Integr. Genomics11491-4962011--1-1--------3-----2-----------------1--1--------------2------------1331--
716038MorkunasCross-talk interactions of suc ...Lupinus luteus, Lupinus luteus PoloJ. Plant Physiol.168424-4332011--------2----5-----3--------------------------2--------3------------2222--
716505ChengMolecular cloning and function ...Ginkgo bilobaPlant Cell Rep.3049-622011--1--------1-4--1--2--111---1----1---1-----------1---1-2--111---1--11331--
716640QinCloning and functional charact ...Trigonella foenum-graecumPlanta Med.77765-7702011--1----------3-----3--1--------------1-----------------3--1----------22---
706399NiuCoordinated regulation of anth ...Morella rubraPlanta231887-8992010-------------3-----4--1--------------------------------4--1---------------
716912Wang-Expression of chalcone synthas ...Citrus unshiu, Citrus unshiu Guoqing No. 4 satsumaSci. Hortic.125110-1162010--2----------2-----8-----------------2-----------------8------------------
704763ParkEngineering of plant-specific ...Medicago sativaJ. Biotechnol.141181-1882009--1----------8--------1--------------1--------------------1---------------
706189ZhangEnhanced flavonoid production ...Glycyrrhiza uralensisPlant Cell Rep.281205-121320091-1----------5-----1--2------------1-1-----------------1--2---------------
690299Chen-Changes of chlorophyll and ant ...Brassica oleracea var. alboglabraActa Hort.769103-1112008------1------1-----1-----------------------1-----------1------------------
692304YamazakiDifferential gene expression p ...Perilla frutescensFEBS J.2753494-35022008--1----------4-----1--1--------------1-----------------1--1---------------
693416PanPhenylalanine ammonia lyase fu ...Astragalus membranaceusJ. Exp. Bot.593027-30372008-------------3-----1--1--------------------------------1--1---------------
694697ChiouDifferential expression of MYB ...Oncidium Gower RamseyPlant Mol. Biol.66379-3882008--1--------1-4-----1--2--------------1-----------1-----1--2---------------
701213Nakatsuka-Isolation and expression analy ...Rhododendron x pulchrumSci. Hortic.118314-3202008--1----------1-----4-----------------1-----------------4------------1111--
676288ChemlerCombinatorial mutasynthesis of ...Scutellaria baicalensisOrg. Lett.91855-18582007-33----------2--------6-------------33--------------------6---------------
677970XiaoCotton flavonoid structural ge ...Gossypium hirsutum, Gossypium hirsutum T586Biochem. Biophys. Res. Commun.35873-782007--2----------3-----2-----------------2-----------------2------------------
679173KatsuyamaSynthesis of unnatural flavono ...Pueraria montana var. lobataChem. Biol.14613-6212007-11----------3----------------------11------------------------------------
680374Ruiz-PerniaEnzymatic effects on reactant ...Medicago sativaJ. Am. Chem. Soc.1299117-91242007-------4-----1--------2----------------------4------------2---------------
681888KimExpression of a functional typ ...Elaeagnus umbellataMol. Cell23405-4092007--1-1--------4-----6-----------------1--1--------------6------------------
663049LiOverexpression of the Saussure ...Saussurea medusaPhytochemistry67553-5602006--1--------1-8-----2--1--------------1-----------1-----2--1---------------
681592Ruiz-PerniaComparative computational anal ...Medicago sativaJ. Phys. Chem. B11020686-206922006-------------2---1--------------------------------------------------------
682437Li-Cloning of a cDNA encoding the ...Saussurea medusaPlant Physiol. Biochem.44455-4612006-21----------1---------1---------1--21---------------------1-------1------
661770NishiharaFlavonoid components and flowe ...Nicotiana tabacumFEBS Lett.5796074-60782005--1--------1-3-----4--1--------------1-----------1-----4--1---------------
663135RalstonPartial reconstruction of flav ...Glycine maxPlant Physiol.1371375-13882005--1----15-----3--1-----15----15---------3-------15-------3----15----15----------
653599HurTransition state stabilization ...Medicago sativaProc. Natl. Acad. Sci. USA1012730-27352004---4---------3-------------------------4----------------------------------
653813GensheimerChalcone isomerase family and ...Cupriavidus metallidurans, Haemophilus influenzae, Haemophilus influenzae RD, Neurospora crassa, Pasteurella multocida, Pseudomonas fluorescens, Pseudomonas fluorescens PfO-1, Pseudomonas putida, Pseudomonas syringae, Saccharomyces cerevisiae, Schizosaccharomyces pombe, Shewanella oneidensis, Vibrio cholerae, Vibrio parahaemolyticusProtein Sci.13540-5442004-------------14------------------------------------------------------------
660833HerlesFirst bacterial chalcone isome ...Eubacterium ramulusArch. Microbiol.181428-4342004------53--2--4--1---1-311--31--------------5-3--2----1--1-311--31---------
662876KimGold color in onions (Allium c ...Allium cepaMol. Genet. Genomics272411-4192004-----------1-1--------1--------------------------1--------1---------------
652235JezReaction mechanism of chalcone ...Medicago sativaJ. Biol. Chem.2771361-13692002--4----4--4--2--4-----44---21-1-------4-------4--4----4----44---21-1--------
653468KimuraChalcone isomerase isozymes wi ...Glycyrrhiza echinataPlant Cell Physiol.421169-11732001-----------2-2--1-----3--------------------------2---1----3---------------
653315JezStructure and mechanism of the ...Medicago sativaNat. Struct. Biol.7786-7912000---4-------1-3--------2----------------4---------1--------2---------------
3737CainExpression of chalcone synthas ...Arabidopsis thalianaPlant Mol. Biol.35377-3811997--1--------1-6--1--1--1--------------1-----------1---1-1--1---------------
3736TeraiCloning of chalcone-flavanone ...Pueraria montana var. lobataProtein Expr. Purif.8183-1901996--1-1-3------5--1---1----------------1--1--3---------1--1-----------------
3738NiStress responses in alfalfa (M ...Medicago sativaPlant Mol. Biol.30427-4381996-----------1-2-----1--1--------------------------1-----1--1---------------
603LiuEnzymes of B-ring-deoxy flavon ...Cephalocereus senilis, Echinocactus horizonthalonius, Myrtillocactus geometrizans, no activity in Stenocereus thurberi, Opuntia sp., Petunia x hybrida, Phaseolus vulgarisArch. Biochem. Biophys.321397-4041995-------4-----9-----7--7----------------------4---------7--7---------------
3735Fouche-Chalcone isomerase from Citrus ...Citrus sinensisPhytochemistry37127-1321994------31--1--1--1--11-11---11--------------3-1--1----1-11-11---11---------
3744McKhannIsolation of chalcone synthase ...Medicago sativaPlant Mol. Biol.24767-7771994--4--------1-5-----5--1--------------4-----------1-----5--1---------------
3739BednarIntroduction of unnatural amin ...Glycine maxBioconjugate Chem.2211-2161991----3-24-----2--------3----10------------3--2-4------------3----10----------
3741BednarChemical modification of chalc ...Glycine maxArch. Biochem. Biophys.282393-3981990------3------3-----------------------------3------------------------------
3743BednarChemical modification of chalc ...Glycine maxJ. Biol. Chem.26414272-142761989------4------3-----------------------------4------------------------------
3733AyabeNAD(P)H-dependent 6-deoxychalc ...Glycyrrhiza echinataArch. Biochem. Biophys.261458-4621988-----------1-4--------3--------------------------1--------3---------------
3734BednarPurification and characterizat ...Glycine maxJ. Biol. Chem.2639582-95881988-------1--1--3-31---1-11---3-----------------1--1---31--1-11---3----------
3746Van TunenCloning of the chalcone isomer ...Petunia x hybridaEMBO J.71257-12631988--1--------1-1--------1--------------1-----------1--------1---------------
3731MehdyChalcone isomerase cDNA clonin ...Phaseolus vulgarisEMBO J.61527-15331987--1--------1-3-----2--1--------------1-----------1-----2--1---------------
3732Van TunenA novel purification procedure ...Petunia x hybridaArch. Biochem. Biophys.25785-911987----------1--1--1--11--1------------------------1----1-11--1--------------
3729Gerrish-trans-Cinnamic acid as a modul ...Phaseolus vulgarisPlant Sci.3823-271985-----------1-1-----1--1--------------------------1-----1--1---------------
3730Mol-Spontaneous and enzymatic rear ...Petunia x hybridaPhytochemistry242267-22691985-1-----------1--------1-----2-------1---------------------1-----2---------
3740RobbinsInduction of chalcone isomeras ...Phaseolus vulgarisEur. J. Biochem.145195-2021984-----------2-5--1--2--22-------------------------2---1-2--22--------------
3745Grotewold-Isolation and characterization ...Zea maysMol. Gen. Genet.2421-81984--1----------1-----------------------1------------------------------------
3724Hinderer-Differentiation of metabolic p ...Daucus carotaPhytochemistry222417-24201983-------------1-----2-----------------------------------2------------------
3728Van Weely-Chalcone isomerase in flowers ...Petunia x hybridaPlanta159226-2301983----------2--1-----3----------------------------2------3------------------
3727Dixon-Purification and properties of ...Phaseolus vulgarisBiochim. Biophys. Acta71525-331982------153--1--1--1--1-231----1--------------15-3--1----1-1-231----1---------
3726Hrazdina-Subcellular localization of en ...Hippeastrum sp.Phytochemistry1753-561978--------1--1-1-----1--1-----------------------1--1-----1--1---------------
3747Wellmann-Induction of anthocyanin forma ...Xanthisma gracilePhytochemistry15913-9151976-----------1-1-----1--1--------------------------1-----1--1---------------
3723HahlbrockLight-induced changes of enzym ...Petroselinum crispumArch. Biochem. Biophys.16641-461975-----------1-1-----1--1--------------------------1-----1--1---------------
3725BolandPurification and kinetic prope ...Glycine maxEur. J. Biochem.50383-3891975------102--11-3--1--21-3----31--------------10-2--11---1-21-3----31---------
3722Wiermann-Aktivität der Chalkon-Flavanon ...Lilium candidum, Tulipa hybrid cultivarPlanta10255-601972-----------3-2-----3--9--------------------------3-----3--9---------------
3721Moustafa-Purification and properties of ...Soja hispidaPhytochemistry6625-6321967------2------1--1---114-----1--------------2---------1--114-----1---------