An unusual plant triterpene synthase with predominant alpha-amyrin-producing activity identified by characterizing oxidosqualene cyclases from Malus × domestica

Brendolise, C.; Yauk, Y.K.; Eberhard, E.D.; Wang, M.; Chagne, D.; Andre, C.; Greenwood, D.R.; Beuning, L.L.; FEBS J. 278, 2485-2499 (2011)

Data extracted from this reference:

Cloned(Commentary)

Commentary	Organism
expressed by transient expression in Nicotiana benthamiana leaves and by expression in the yeast Pichia methanolica	Malus x domestica

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Commentary (Nat. Sub.)	Natural Products	Commentary (Nat. Pro.)	Organism (Nat. Pro.)	Reversibility
(3S)-2,3-epoxy-2,3-dihydrosqualene	Malus x domestica	the product alpha-amyrin is a precursor of ursolic acid	alpha-amyrin	-	-	?

Organism

Organism	Primary Accession No. (UniProt)	Commentary	Textmining
Malus x domestica	C7SCX0	Royal Gala	-

Source Tissue

Source Tissue	Commentary	Organism	Textmining
exocarp	-	Malus x domestica	-
fruit	exocarp	Malus x domestica	-

Substrates and Products (Substrate)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
(3S)-2,3-epoxy-2,3-dihydrosqualene	the product alpha-amyrin is a precursor of ursolic acid	711990	Malus x domestica	alpha-amyrin	-	-	-	?
(3S)-2,3-epoxy-2,3-dihydrosqualene	the mixed amyrin synthase produces both alpha- and beta-amyrin in a ration of 5:1	711990	Malus x domestica	alpha-amyrin	-	-	-	?

Cloned(Commentary) (protein specific)

Commentary	Organism
expressed by transient expression in Nicotiana benthamiana leaves and by expression in the yeast Pichia methanolica	Malus x domestica

Natural Substrates/ Products (Substrates) (protein specific)

Natural Substrates	Organism	Commentary (Nat. Sub.)	Natural Products	Commentary (Nat. Pro.)	Organism (Nat. Pro.)	Reversibility
(3S)-2,3-epoxy-2,3-dihydrosqualene	Malus x domestica	the product alpha-amyrin is a precursor of ursolic acid	alpha-amyrin	-	-	?

Source Tissue (protein specific)

Source Tissue	Commentary	Organism	Textmining
exocarp	-	Malus x domestica	-
fruit	exocarp	Malus x domestica	-

Substrates and Products (Substrate) (protein specific)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
(3S)-2,3-epoxy-2,3-dihydrosqualene	the product alpha-amyrin is a precursor of ursolic acid	711990	Malus x domestica	alpha-amyrin	-	-	-	?
(3S)-2,3-epoxy-2,3-dihydrosqualene	the mixed amyrin synthase produces both alpha- and beta-amyrin in a ration of 5:1	711990	Malus x domestica	alpha-amyrin	-	-	-	?

See also following references to EC number 5.4.99.40 (sorted by year of publication):

No.	1st author	Pub Med	title	organims	journal	volume	pages	year	Activating Compound	Application	Cloned(Commentary)	Crystallization (Commentary)	Engineering	General Stability	Inhibitors	KM Value [mM]	Localization	Metals/Ions	Molecular Weight [Da]	Natural Substrates/ Products (Substrates)	Organic Solvent Stability	Organism	Oxidation Stability	Posttranslational Modification	Purification (Commentary)	Reaction	Renatured (Commentary)	Source Tissue	Specific Activity [micromol/min/mg]	Storage Stability	Substrates and Products (Substrate)	Subunits	Temperature Optimum [°C]	Temperature Range [°C]	Temperature Stability [°C]	Turnover Number [1/s]	pH Optimum	pH Range	pH Stability	Cofactor	Ki Value [mM]	pI Value	IC50 Value	Activating Compound (protein specific)	Application (protein specific)	Cloned(Commentary) (protein specific)	Cofactor (protein specific)	Crystallization (Commentary) (protein specific)	Engineering (protein specific)	General Stability (protein specific)	IC50 Value (protein specific)	Inhibitors (protein specific)	Ki Value [mM] (protein specific)	KM Value [mM] (protein specific)	Localization (protein specific)	Metals/Ions (protein specific)	Molecular Weight [Da] (protein specific)	Natural Substrates/ Products (Substrates) (protein specific)	Organic Solvent Stability (protein specific)	Oxidation Stability (protein specific)	Posttranslational Modification (protein specific)	Purification (Commentary) (protein specific)	Renatured (Commentary) (protein specific)	Source Tissue (protein specific)	Specific Activity [micromol/min/mg] (protein specific)	Storage Stability (protein specific)	Substrates and Products (Substrate) (protein specific)	Subunits (protein specific)	Temperature Optimum [°C] (protein specific)	Temperature Range [°C] (protein specific)	Temperature Stability [°C] (protein specific)	Turnover Number [1/s] (protein specific)	pH Optimum (protein specific)	pH Range (protein specific)	pH Stability (protein specific)	pI Value (protein specific)	Expression	General Information	General Information (protein specific)	Expression (protein specific)	KCat/KM [mM/s]	KCat/KM [mM/s] (protein specific)
711990	Brendolise		An unusual plant triterpene sy ...	Malus x domestica	FEBS J.	278	2485-2499	2011	-	-	1	-	-	-	-	-	-	-	-	1	-	7	-	-	-	-	-	2	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	2	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
716616	Wang		Two oxidosqualene cyclases res ...	Solanum lycopersicum	Plant Physiol.	155	540-552	2011	-	-	1	-	-	-	-	-	-	-	-	-	-	1	-	-	1	-	-	1	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	1	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
683422	Morita		Molecular cloning and function ...	Pisum sativum	Eur. J. Biochem.	267	3453-3460	2000	-	-	1	-	-	-	-	-	-	-	-	1	-	4	-	-	-	1	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-