Crystallization and initial crystallographic analysis of phosphoglucosamine mutase from Bacillus anthracis

Mehra-Chaudhary, R.; Neace, C.E.; Beamer, L.J.; Acta Crystallogr. Sect. F 65, 733-735 (2009)

Data extracted from this reference:

Cloned(Commentary)

Commentary	Organism
into the pDONR221 vector and subsequently into the pDEST17 vector for expression in Escherichia coli BL21DE3 cells	Bacillus anthracis

Crystallization (Commentary)

Crystallization	Organism
the crystal structure of Bacillus anthracis phosphoglucosamine mutase is determined to a resolution of 2.7 A	Bacillus anthracis

Localization

Localization	Commentary	Organism	GeneOntology No.	Textmining
cytoplasm	-	Bacillus anthracis	5737	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Commentary	Organism
50960	-	-	Bacillus anthracis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Commentary (Nat. Sub.)	Natural Products	Commentary (Nat. Pro.)	Organism (Nat. Pro.)	Reversibility
alpha-D-glucosamine 1-phosphate	Bacillus anthracis	-	D-glucosamine 6-phosphate	-	-	?

Organism

Organism	Primary Accession No. (UniProt)	Commentary	Textmining
Bacillus anthracis	-	-	-

Purification (Commentary)

Commentary	Organism
using His-Select Ni-Affinity gel	Bacillus anthracis

Substrates and Products (Substrate)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
alpha-D-glucosamine 1-phosphate	-	701529	Bacillus anthracis	D-glucosamine 6-phosphate	-	-	-	?

Cloned(Commentary) (protein specific)

Commentary	Organism
into the pDONR221 vector and subsequently into the pDEST17 vector for expression in Escherichia coli BL21DE3 cells	Bacillus anthracis

Crystallization (Commentary) (protein specific)

Crystallization	Organism
the crystal structure of Bacillus anthracis phosphoglucosamine mutase is determined to a resolution of 2.7 A	Bacillus anthracis

Localization (protein specific)

Localization	Commentary	Organism	GeneOntology No.	Textmining
cytoplasm	-	Bacillus anthracis	5737	-

Molecular Weight [Da] (protein specific)

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Commentary	Organism
50960	-	-	Bacillus anthracis

Natural Substrates/ Products (Substrates) (protein specific)

Natural Substrates	Organism	Commentary (Nat. Sub.)	Natural Products	Commentary (Nat. Pro.)	Organism (Nat. Pro.)	Reversibility
alpha-D-glucosamine 1-phosphate	Bacillus anthracis	-	D-glucosamine 6-phosphate	-	-	?

Purification (Commentary) (protein specific)

Commentary	Organism
using His-Select Ni-Affinity gel	Bacillus anthracis

Substrates and Products (Substrate) (protein specific)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
alpha-D-glucosamine 1-phosphate	-	701529	Bacillus anthracis	D-glucosamine 6-phosphate	-	-	-	?

General Information

General Information	Commentary	Organism
physiological function	phosphoglucosamine mutase catalyzes the conversion of glucosamine 6-phosphate to glucosamine 1-phosphate, an early step in the formation of the nucleotide sugar UDP-N-acetylglucosamine, which is involved in peptidoglycan biosynthesis	Bacillus anthracis

General Information (protein specific)

General Information	Commentary	Organism
physiological function	phosphoglucosamine mutase catalyzes the conversion of glucosamine 6-phosphate to glucosamine 1-phosphate, an early step in the formation of the nucleotide sugar UDP-N-acetylglucosamine, which is involved in peptidoglycan biosynthesis	Bacillus anthracis

See also following references to EC number 5.4.2.10 (sorted by year of publication):

No.	1st author	Pub Med	title	organims	journal	volume	pages	year	Activating Compound	Application	Cloned(Commentary)	Crystallization (Commentary)	Engineering	General Stability	Inhibitors	KM Value [mM]	Localization	Metals/Ions	Molecular Weight [Da]	Natural Substrates/ Products (Substrates)	Organic Solvent Stability	Organism	Oxidation Stability	Posttranslational Modification	Purification (Commentary)	Reaction	Renatured (Commentary)	Source Tissue	Specific Activity [micromol/min/mg]	Storage Stability	Substrates and Products (Substrate)	Subunits	Temperature Optimum [°C]	Temperature Range [°C]	Temperature Stability [°C]	Turnover Number [1/s]	pH Optimum	pH Range	pH Stability	Cofactor	Ki Value [mM]	pI Value	IC50 Value	Activating Compound (protein specific)	Application (protein specific)	Cloned(Commentary) (protein specific)	Cofactor (protein specific)	Crystallization (Commentary) (protein specific)	Engineering (protein specific)	General Stability (protein specific)	IC50 Value (protein specific)	Inhibitors (protein specific)	Ki Value [mM] (protein specific)	KM Value [mM] (protein specific)	Localization (protein specific)	Metals/Ions (protein specific)	Molecular Weight [Da] (protein specific)	Natural Substrates/ Products (Substrates) (protein specific)	Organic Solvent Stability (protein specific)	Oxidation Stability (protein specific)	Posttranslational Modification (protein specific)	Purification (Commentary) (protein specific)	Renatured (Commentary) (protein specific)	Source Tissue (protein specific)	Specific Activity [micromol/min/mg] (protein specific)	Storage Stability (protein specific)	Substrates and Products (Substrate) (protein specific)	Subunits (protein specific)	Temperature Optimum [°C] (protein specific)	Temperature Range [°C] (protein specific)	Temperature Stability [°C] (protein specific)	Turnover Number [1/s] (protein specific)	pH Optimum (protein specific)	pH Range (protein specific)	pH Stability (protein specific)	pI Value (protein specific)	Expression	General Information	General Information (protein specific)	Expression (protein specific)	KCat/KM [mM/s]	KCat/KM [mM/s] (protein specific)
716403	Shimazu		Contribution of phosphoglucosa ...	Streptococcus gordonii	Odontology	100	28-33	2012	-	-	-	-	-	-	-	-	-	-	-	1	-	5	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	2	2	-	-	-
715384	Mehra-Chaudhary		Crystal structure of phosphogl ...	Bacillus anthracis	J. Bacteriol.	193	4081-4087	2011	-	-	-	-	-	-	-	-	1	-	1	-	-	4	-	-	-	-	-	-	-	-	1	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	1	-	-	-	-	-	-	-	-	-	1	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-
701529	Mehra-Chaudhary		Crystallization and initial cr ...	Bacillus anthracis	Acta Crystallogr. Sect. F	65	733-735	2009	-	-	1	1	-	-	-	-	1	-	1	1	-	3	-	-	1	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	1	-	-	-	-	-	-	1	-	1	1	-	-	-	1	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	1	1	-	-	-
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