Acetamido sugar biosynthesis in the Euryarchaea

Namboori, S.C.; Graham, D.E.; J. Bacteriol. 190, 2987-2996 (2008)

 show all sequences of 5.4.2.10

Data extracted from this reference:

Activating Compound
Activating Compound Commentary Organism Structure
D-fructose-1,6-bisphosphate required to activate the enzyme Methanococcus maripaludis
dithiothreitol 1 mM, required for maximal activity Methanococcus maripaludis
additional information no activity is observed with ATP as an activator Methanococcus maripaludis
-
Cloned(Commentary)
Commentary Organism
expressed in Escherichia coli BL21(DE3) cells Methanococcus maripaludis
Metals/Ions
Metals/Ions Commentary Organism Structure
Mg2+ activity is 95% lower when MgCl2 is used instead of MnCl2 Methanococcus maripaludis
Mn2+ required for activity Methanococcus maripaludis
Molecular Weight [Da]
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
50000
-
 SDS-PAGE Methanococcus maripaludis
340000
-
 gel filtration Methanococcus maripaludis
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Methanococcus maripaludis Q6LYB4 strain 900
-
Methanococcus maripaludis 900 Q6LYB4 strain 900
-
Purification (Commentary)
Commentary Organism
Ni+ affinity column chromatography Methanococcus maripaludis
Substrates and Products (Substrate)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
alpha-D-glucosamine 6-phosphate
-
 692855 Methanococcus maripaludis alpha-D-glucosamine 1-phosphate
-
-
-
 r
alpha-D-glucose 1-phosphate
-
 692855 Methanococcus maripaludis alpha-D-glucose 6-phosphate
-
-
-
 ?
Turnover Number [1/s]
Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Commentary Organism Structure
0.1
-
 alpha-D-glucose 1-phosphate
-
 Methanococcus maripaludis
0.52
-
 alpha-D-glucosamine 1-phosphate
-
 Methanococcus maripaludis
Activating Compound (protein specific)
Activating Compound Commentary Organism Structure
D-fructose-1,6-bisphosphate required to activate the enzyme Methanococcus maripaludis
dithiothreitol 1 mM, required for maximal activity Methanococcus maripaludis
additional information no activity is observed with ATP as an activator Methanococcus maripaludis
-
Cloned(Commentary) (protein specific)
Commentary Organism
expressed in Escherichia coli BL21(DE3) cells Methanococcus maripaludis
Metals/Ions (protein specific)
Metals/Ions Commentary Organism Structure
Mg2+ activity is 95% lower when MgCl2 is used instead of MnCl2 Methanococcus maripaludis
Mn2+ required for activity Methanococcus maripaludis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
50000
-
 SDS-PAGE Methanococcus maripaludis
340000
-
 gel filtration Methanococcus maripaludis
Purification (Commentary) (protein specific)
Commentary Organism
Ni+ affinity column chromatography Methanococcus maripaludis
Substrates and Products (Substrate) (protein specific)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
alpha-D-glucosamine 6-phosphate
-
 692855 Methanococcus maripaludis alpha-D-glucosamine 1-phosphate
-
-
-
 r
alpha-D-glucose 1-phosphate
-
 692855 Methanococcus maripaludis alpha-D-glucose 6-phosphate
-
-
-
 ?
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Commentary Organism Structure
0.1
-
 alpha-D-glucose 1-phosphate
-
 Methanococcus maripaludis
0.52
-
 alpha-D-glucosamine 1-phosphate
-
 Methanococcus maripaludis

See also following references to EC number 5.4.2.10 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
716403ShimazuContribution of phosphoglucosa ...Streptococcus gordoniiOdontology10028-332012-----------1-5--------1--------------------------1--------1----------22---
715384Mehra-ChaudharyCrystal structure of phosphogl ...Bacillus anthracisJ. Bacteriol.1934081-40872011--------1-1--4--------11----------------------1-1---------11--------------
701529Mehra-ChaudharyCrystallization and initial cr ...Bacillus anthracisActa Crystallogr. Sect. F65733-7352009--11----1-11-3--1-----1--------------1-1------1-11---1----1----------11---
703755YajimaContribution of phosphoglucosa ...Streptococcus gordoniiFEMS Microbiol. Lett.297196-2022009-1--1------1-5-----1--1-------------1---1--------1-----1--1----------11---
706029LiuRole of phosphoglucosamine mut ...Streptococcus mutansOral Microbiol. Immunol.24272-2772009-11-1------1-4--------1-------------11--1--------1--------1---------1111--
692372ShimazuIdentification of the Streptoc ...Streptococcus gordoniiFEMS Immunol. Med. Microbiol.53166-1772008--1-------2--6--1-----1--------------1----------2----1----1---------------
692855NambooriAcetamido sugar biosynthesis i ...Methanococcus maripaludisJ. Bacteriol.1902987-299620083-1------22--5--1-----2----2-------3-1---------22----1----2----2----------
210869TavaresIdentification of the Pseudomo ...Pseudomonas aeruginosaJ. Bacteriol.1824453-44572000--1--------1-5--1---3-3--------------1-----------1---1--3-3---------------
210870JollyAutophosphorylation of phospho ...Escherichia coliJ. Bacteriol.1821280-128520001-1-2-4--1-2-5-11--1--2------------1-1--2--4---1-2--11-1--2---------------
210871JollyReaction mechanism of phosphog ...Escherichia coliEur. J. Biochem.262202-21019992-1-3--6---5-2-11--13-61---4-------2-1--3----6---5--11-13-61---4----------
210872JollyThe femR315 gene from Staphylo ...Staphylococcus aureusJ. Bacteriol.1795321-532519971-1--------1-3--1---1-1------------1-1-----------1---1--1-1---------------
210873De ReuseThe Helicobacter pylori ureC g ...Helicobacter pyloriJ. Bacteriol.1793488-34931997--1--------1-9--1---1-1--------------1-----------1---1--1-1---------------
210874Mengin-LecreulxCharacterization of the essent ...Escherichia coliJ. Biol. Chem.27132-3919961-1--------1-3-11--11-1------------1-1-----------1--11-11-1---------------