Isoprenoid enzyme systems of silkworm. I. Partial purification of isopentenyl pyrophosphate isomerase, farnesyl pyrophosphate synthetase, and geranylgeranyl pyrophosphate synthetase

Koyama, T.; Matsubara, M.; Ogura, K.; J. Biochem. 98, 449-456 (1985)

 show all sequences of 5.3.3.2

Data extracted from this reference:

KM Value [mM]
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
0.02
-
 isopentenyl diphosphate
-
 Bombyx mori
0.02
-
 isopentenyl diphosphate
-
 Bombyx mori 122
0.02
-
 isopentenyl diphosphate
-
 Bombyx mori Bm-N
0.02
-
 isopentenyl diphosphate
-
 Bombyx mori C108
0.02
-
 isopentenyl diphosphate
-
 Bombyx mori P21
0.02
-
 isopentenyl diphosphate
-
 Bombyx mori P22
0.02
-
 isopentenyl diphosphate
-
 Bombyx mori Shogetu-Shunrei
Metals/Ions
Metals/Ions Commentary Organism Structure
Mg2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori
Mg2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori 122
Mg2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori Bm-N
Mg2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori C108
Mg2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori P21
Mg2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori P22
Mg2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori Shogetu-Shunrei
Mn2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori
Mn2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori 122
Mn2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori Bm-N
Mn2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori C108
Mn2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori P21
Mn2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori P22
Mn2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori Shogetu-Shunrei
Molecular Weight [Da]
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
35000
-
 gel filtration Bombyx mori 122
35000
-
 gel filtration Bombyx mori Bm-N
35000
-
 gel filtration Bombyx mori C108
35000
-
 gel filtration Bombyx mori
35000
-
 gel filtration Bombyx mori P21
35000
-
 gel filtration Bombyx mori P22
35000
-
 gel filtration Bombyx mori Shogetu-Shunrei
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Bombyx mori
-
-
-
Purification (Commentary)
Commentary Organism
partial Bombyx mori 122
partial Bombyx mori Bm-N
partial Bombyx mori C108
partial Bombyx mori P21
partial Bombyx mori P22
partial Bombyx mori
partial Bombyx mori Shogetu-Shunrei
Substrates and Products (Substrate)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
Isopentenyl diphosphate
-
 2942 Bombyx mori Dimethylallyl diphosphate
-
 2942 Bombyx mori
-
pH Optimum
pH Optimum Minimum pH Optimum Maximum Commentary Organism
7 7.8 isomerase I Bombyx mori
7 8 isomerase II Bombyx mori
KM Value [mM] (protein specific)
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
0.02
-
 isopentenyl diphosphate
-
 Bombyx mori
0.02
-
 isopentenyl diphosphate
-
 Bombyx mori 122
0.02
-
 isopentenyl diphosphate
-
 Bombyx mori Bm-N
0.02
-
 isopentenyl diphosphate
-
 Bombyx mori C108
0.02
-
 isopentenyl diphosphate
-
 Bombyx mori P21
0.02
-
 isopentenyl diphosphate
-
 Bombyx mori P22
0.02
-
 isopentenyl diphosphate
-
 Bombyx mori Shogetu-Shunrei
Metals/Ions (protein specific)
Metals/Ions Commentary Organism Structure
Mg2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori
Mg2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori 122
Mg2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori Bm-N
Mg2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori C108
Mg2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori P21
Mg2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori P22
Mg2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori Shogetu-Shunrei
Mn2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori
Mn2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori 122
Mn2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori Bm-N
Mn2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori C108
Mn2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori P21
Mn2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori P22
Mn2+ Mn2+ is a better activator than Mg2+; Mn2+ or Mg2+ required Bombyx mori Shogetu-Shunrei
Molecular Weight [Da] (protein specific)
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
35000
-
 gel filtration Bombyx mori 122
35000
-
 gel filtration Bombyx mori
35000
-
 gel filtration Bombyx mori Bm-N
35000
-
 gel filtration Bombyx mori C108
35000
-
 gel filtration Bombyx mori P21
35000
-
 gel filtration Bombyx mori P22
35000
-
 gel filtration Bombyx mori Shogetu-Shunrei
Purification (Commentary) (protein specific)
Commentary Organism
partial Bombyx mori 122
partial Bombyx mori
partial Bombyx mori Bm-N
partial Bombyx mori C108
partial Bombyx mori P21
partial Bombyx mori P22
partial Bombyx mori Shogetu-Shunrei
Substrates and Products (Substrate) (protein specific)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
Isopentenyl diphosphate
-
 2942 Bombyx mori Dimethylallyl diphosphate
-
 2942 Bombyx mori
-
pH Optimum (protein specific)
pH Optimum Minimum pH Optimum Maximum Commentary Organism
7 7.8 isomerase I Bombyx mori
7 8 isomerase II Bombyx mori

See also following references to EC number 5.3.3.2 (sorted by year of publication):
No.1st authorPub
Med		titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
719740NakataniSubstrate-induced change in th ...Sulfolobus shibataeJ. Bacteriol.1943216-32242012--111--2---1-4--1-----111---1--------1-11----2---1---1----111---1---------
713655HoshinoCrystallization and preliminar ...Methanocaldococcus jannaschiiActa Crystallogr. Sect. F67101-1032011--11--1--6---3--1-----11-------1-----111---1---6-----1----11--------------
706249Sun-A novel cytoplasmic isopenteny ...Solanum lycopersicumPlant Mol. Biol. Rep.28473-4802010-11-----1-21-1-----5--1----------1--11--------1-21-----5--1--------1-11---
714202SharmaType II isopentenyl diphosphat ...Escherichia coli, Thermus thermophilusBiochemistry496228-62332010--2---4--3---4--------4--------134----213----44--3----------4---------------
715286ThibodeauxLinear free energy relationshi ...Staphylococcus aureusJ. Am. Chem. Soc.1329994-99962010----1--------1--------1--------55------55-1-----------------1---------------
691400WangMolecular cloning, expression ...Gossypium barbadenseBiosci. Rep.29111-1192009--1-1--------6-----4---1---------1---1--1--------------4---1-------1------
701815GhimireImproved squalene production v ...Escherichia coliAppl. Environ. Microbiol.757291-72932009-11--------1-4--------1-------------11-----------1--------1---------------
702750OhtoPrenyl alcohol production by e ...Escherichia coliBiosci. Biotechnol. Biochem.73186-1882009-11--------1-2--------1-------------11-----------1--------1---------------
704638UnnoNew role of flavin as a genera ...Sulfolobus shibataeJ. Biol. Chem.2849160-91672009--1115----1-1-3--1---1-1--------2-----12115------1-1---1--1-1---------------
705515LaiReconstruction of the archaeal ...Escherichia coliMetab. Eng.11184-1912009-11--------1-1--1-----1-------------11-----------1---1----1---------------
706809BattilanaThe 1-deoxy-D: -xylulose 5-pho ...Vitis vinifera x Vitis riparia, Vitis vinifera x Vitis viniferaTheor. Appl. Genet.118653-6692009-------------2-------------------------------------------------------22---
690288de RuyckMonoclinic form of isopentenyl ...Escherichia coliActa Crystallogr. Sect. FF64239-2422008--11---------1-----------------------1-1----------------------------------
690701FujiwaraEfficient synthesis of trans-p ...Thermococcus kodakarensisBiochem. Biophys. Res. Commun.365118-1232008-2-----------4----------------------2-------------------------------------
690929ThibodeauxEvidence for the involvement o ...Staphylococcus aureusBiochemistry472547-25582008-------------3---55----1-----------------------------------1---------------
690998de RuyckCrystal structure of type 2 is ...Thermus thermophilusBiochemistry479051-90532008---1---------3-------------------------1----------------------------------
691183Liao-A new isopentenyl diphosphate ...Ipomoea batatasBiologia63221-2262008--1-1--------1-----3---1---------1---1--1--------------3---1-------1------
692027PanA new isopentenyl diphosphate ...Camptotheca acuminataDNA Seq.1998-1052008--1-1--------3-----4-----------------1--1--------------4------------------
692765RothmanType II isopentenyl diphosphat ...Thermus thermophilusJ. Am. Chem. Soc.1304906-49132008--1---6------2--------4---------6----1-----66-------------4---------------
693801WalkerSynthesis and evaluation of su ...Thermus thermophilusJ. Org. Chem.73726-7292008------3------2------------------1-36-------3631-----------------------------
694638PhillipsThe Arabidopsis thaliana type ...Arabidopsis thalianaPlant Cell20677-6962008----1---3----5--------------------------2-----4---------------------------
694654OkadaGenetic evidence for the role ...Arabidopsis thalianaPlant Cell Physiol.49604-6162008----1--------2--------------------------2---------------------------------
695056DutoitOverexpression, physicochemica ...Pyrococcus furiosusProteins Struct. Funct. Bioinform.711699-17072008--11------1--5---------1--1----------1-1--------1----------1--1-----------
678278RothmanKinetic and spectroscopic char ...Thermus thermophilusBiochemistry465437-54452007------12-1---5--1-12---2----1---13------13----1-2-1-----112---2----1----------
678309KittlemanCharacterization and mechanist ...Staphylococcus aureus, Staphylococcus aureus IDI-2Biochemistry468401-84132007------13-----7----55---1----3---110------110----1-3--------55---1----3----------
680372JohnstonType-2 isopentenyl diphosphate ...Thermus thermophilusJ. Am. Chem. Soc.1297740-77412007------2------1-----------------------------2------------------------------
680882ClizbeIDI2, a second isopentenyl dip ...Homo sapiensJ. Biol. Chem.2826668-66762007----1--111---5-----11-1-----------------1----111-------11-1---------------
681412ZhangCrystal structures of human IP ...Homo sapiensJ. Mol. Biol.3661437-14462007---1--1------4-------------------------1---1------------------------------
681413ZhengThe crystal structure of human ...Homo sapiensJ. Mol. Biol.3661447-14582007---1---------4---1---------------------1----------------------------------
678586HoshinoInhibition of type 2 isopenten ...Methanocaldococcus jannaschiiBioorg. Med. Chem.146555-65592006------11-----3--------116--11---1----------111------------116--11---------
680346LeeEscherichia coli type I isopen ...Escherichia coliJ. Am. Chem. Soc.12811545-115502006---1--1--6---3----1--------------------1---1---6------1-------------------
680660de RuyckStructural role for Tyr-104 in ...Escherichia coliJ. Biol. Chem.28117864-178692006---12--3-----3--------1---3------------12----3------------1---3-----------
660902SiddiquiEnzymatic and structural chara ...Thermococcus kodakarensisBiochem. Biophys. Res. Commun.3311127-11362005--1----11-11--3--1-----11112----4-----14------11-11----1----11112-----------
660922de RuyckStructure of Thermus thermophi ...Thermus thermophilus, Thermus thermophilus HB 27Biochem. Biophys. Res. Commun.3381515-15182005--11---------4--1--------------------1-1-------------1--------------------
661948WoutersA crystallographic investigati ...Escherichia coliJ. Am. Chem. Soc.127536-5372005---1---------1-------------------------1----------------------------------
651170YamashitaType 2 isopentenyl diphosphate ...Sulfolobus shibataeEur. J. Biochem.2711087-10932004--1----3-1---4--1-----21---1---2-----12------3-1-----1----21---1----------
651733BarkleyIdentification of an archaeal ...Methanothermobacter thermautotrophicusJ. Bacteriol.1861811-1817200418-1----1-1-1-7--1-----211--11------18-1-------1-1-1---1----211--11---------
653840WoutersCrystal structure of the C67A ...Escherichia coliProteins54216-2212004---1--1----1-2---1----1----------------1---1-----1--------1---------------
660882HemmiCatalytic mechanism of type 2 ...Sulfolobus shibataeBiochem. Biophys. Res. Commun.322905-9102004------33-3---2--3-----3--------93-----9----333-3-----3----3---------------
661357TakagiBacillus subtilis ypgA gene is ...Bacillus subtilisBiosci. Biotechnol. Biochem.68132-1372004------1--4---7--------1-11--1--2------2----1---4----------1-11--1---------
661638LaupitzBiochemical characterization o ...Bacillus subtilisEur. J. Biochem.2712658-26692004--1------72--8--1---6-2--------3-----13--------72----1--6-2---------------
662026BarkleyType II isopentenyl diphosphat ...Synechocystis sp.J. Bacteriol.1868156-81582004--1------1010--4--1---10-1-----1--20-----120--------1010----1--10-1-----1---------
651594WoutersStructure and mechanism of act ...Escherichia coliJ. Am. Chem. Soc.1253198-31992003---12-3------1-------------------------12--3------------------------------
651597CarriganZinc is an essential cofactor ...Escherichia coliJ. Am. Chem. Soc.1259008-90092003---------3---2--------1------------------------3----------1---------------
652367WoutersCatalytic mechanism of Escheri ...Escherichia coliJ. Biol. Chem.27811903-119082003---1-------1-3---1----1----------------1---------1--------1---------------
652916SteinbacherCrystal structure of the type ...Bacillus subtilisJ. Mol. Biol.329973-9822003---1---------3---------1-------1------11-------------------1--------------
649588ThompsonIdentification of a bisphospho ...Homo sapiensBiochem. Biophys. Res. Commun.290869-8732002------1------2-----------------------------1------------------------------
649155OudjamaPreliminary structural studies ...Escherichia coliActa Crystallogr. Sect. D57287-2882001--11---------2---------1-------------1-1-------------------1--------------
650979DurbecqCrystal structure of isopenten ...Escherichia coliEMBO J.201530-15372001---18--8-2-1-3--11----2----------------18----8-2-1---1----2---------------
638703Ramos-Valdivia-Isopentenyl diphosphate isomer ...Catharanthus roseus, Cinchona robusta, Morinda citrifoliaPhytochemistry48961-9691998-------------3------------------------------------------------------------
2950PatonCloning and subcellular locali ...Mesocricetus auratus, Rattus norvegicusJ. Biol. Chem.27218945-189501997--2-----1----4--------2--------------2--------1-----------2---------------
2952Ramos-ValdiviaPurification and characterizat ...Cinchona robustaEur. J. Biochem.249161-1701997------43-2-1-1--1--11121-----1-------------4-3-2-1---1-11121-----1--------
2953KajiwaraExpression of an exogenous iso ...Haematococcus pluvialis, Phaffia rhodozyma, Saccharomyces cerevisiaeBiochem. J.324421-4261997-99----------8--------9-------------99--------------------9---------------
2948HahnHuman isopentenyl diphosphate: ...Homo sapiensArch. Biochem. Biophys.33230-341996--1----1-2-1-4--1-----2-----1--------1-------1-2-1---1----2-----1---------
2959WandersCholesterol biosynthesis in Ze ...Homo sapiensJ. Inherit. Metab. Dis.19193-1961996-------------2-----3--1--------------------------------3--1---------------
2951HahnIsolation of Schizosaccharomyc ...Schizosaccharomyces pombeJ. Biol. Chem.27011298-113031995--15--------1-5--------1615-------------15-----------1--------1615--------------
2955ChristensenEnzymatic synthesis of isotopi ...Saccharomyces cerevisiaeBioorg. Med. Chem.2631-6371994-1-----------1--------1-------------1---------------------1---------------
2949LuIsopentenyl-diphosphate isomer ...Saccharomyces cerevisiaeBiochemistry319955-99601992------1------2--------1--------------------1--------------1---------------
2954LanznasterDye-ligand immobilized metal i ...Salvia officinalisProtein Expr. Purif.269-741991-------------1--1--1--1------------------------------1-1--1---------------
2956StreetHydrogen exchange during enzym ...Saccharomyces cerevisiaeJ. Am. Chem. Soc.1128577-85781990-----------1-1--------2--------------------------1--------2---------------
2957StreetIsopentenyldiphosphate:dimethy ...Saccharomyces cerevisiaeBiochemistry297531-75381990--1---11---1-5--1---1-2--------------1-----1-1---1---1--1-2---------------
2958AndersonIsopentenyl diphosphate:dimeth ...Saccharomyces cerevisiaeJ. Biol. Chem.26419169-191751989--1---2------4--1---1-11-------------1-----2---------1--1-11--------------
2946Lutzow-The isopentenyl-diphosphate DE ...Narcissus pseudonarcissusBiochim. Biophys. Acta959118-1261988------1112-1-1--1---1-2-----1--------------1-112-1---1--1-2-----1---------
2947MuehlbacherIsopentenyl-diphosphate isomer ...Claviceps purpureaBiochemistry277315-73281988------10------3--2---2-2--------------------10---------2--2-2---------------
2945Dogbo-Purification of isopentenyl py ...Capsicum annuumBiochim. Biophys. Acta920140-1481987-------11-1--1-----11-11---------------------11-1------11-11--------------
2944BruengerIsopentenyl pyrophosphate isom ...Claviceps sp., Gallus gallusArch. Biochem. Biophys.248620-6251986-----6203-103--4--3--13-43--3-1------------6-20-3-83----3-13-43--3-1---------
2939Reardpon-Time-dependent inhibition of i ...Saccharomyces cerevisiaeJ. Am. Chem. Soc.1074078-40791985------41-----1--------1--------------------4-1------------1---------------
2940SatterwhiteIsopentenyldiphosphate DELTA-i ...Claviceps purpurea, Gallus gallusMethods Enzymol.11092-991985-------------2--3--33-3------------------------------3-33-3---------------
2941JonesEnzymatic synthesis of phytoen ...Solanum lycopersicumMethods Enzymol.110209-22019855-----351651-1--5--15-2------------5-------3-51651---5-15-2---------------
2942KoyamaIsoprenoid enzyme systems of s ...Bombyx moriJ. Biochem.98449-4561985-------7-147--3--7-----1-----2----------------7-147----7----1-----2---------
2943Muehlbacher-Isopentenyl diphosphate:dimeth ...Claviceps sp., Claviceps sp. SD58J. Am. Chem. Soc.1078307-83081985------5------2-----3--3--------------------5-----------3--3---------------
2938SpurgeonIsopentenyl pyrophosphate isom ...Solanum lycopersicumArch. Biochem. Biophys.230446-4541984------35265--3--5--2551--------------------3-5265----5-2551---------------
2937SagamiMultiple forms of isopentenyl ...Gallus gallusJ. Biochem.94975-97919831-----23-4---1-----1--1-----1------1-------2-3-4-------1--1-----1---------
2936BanthorpeIsopentenyl pyrophosphate isom ...Sus scrofaArch. Biochem. Biophys.184381-39019776-----191-21--2--1--21-1-----11-----6-------19-1-21----1-21-1-----11--------
2934GreenCompartmentation of isopenteny ...Ricinus communisBiochem. Biophys. Res. Commun.64976-9821975--------3--1-1-----1--2-----------------------3--1-----1--2---------------
2933KoyamaStudies on isopentenyl pyropho ...Rattus norvegicus, Sus scrofaJ. Biol. Chem.2488043-80511973-------------2-----5--4--------------------------------5--4---------------
2935Holloway-Isopentenylpyrophosphate isome ...Acholeplasma laidlawii, Citrus sp., Cucurbita sp., Pinus radiata, Saccharomyces cerevisiae, Sus scrofaThe Enzymes, 3rd Ed. (Boyer, P.D., ed.)6565-5721972------21-1---6-----3--12--------------------2-1-1-------3--12---------------
2932AgranoffBiosynthesis of terpenes. VII. ...Saccharomyces cerevisiaeJ. Biol. Chem.235326-3321960------21-----1--1---111-----11-------------2-1-------1--111-----11--------