Isoenzymes of glutathione S-transferase from the mosquito Anopheles dirus species B: the purification, partial characterization and interaction with various insecticides

Prapanthadara, L.; Promtet, N.; Koottathep, S.; Somboon, P.; Ketterman, A.J.; Insect Biochem. Mol. Biol. 30, 395-403 (2000)

Data extracted from this reference:

Inhibitors

Inhibitors	Commentary	Organism	Structure
bromosulfophthalein	-	Anopheles dirus
Ethacrynic acid	-	Anopheles dirus

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Commentary	Organism	Structure
0.21	-	1-chloro-2,4-dinitrobenzene	pH 6.5, 22°C	Anopheles dirus

Organism

Organism	Primary Accession No. (UniProt)	Commentary	Textmining
Anopheles dirus	-	Thai mosquito, glutathione S-transferase isozyme 4c has enzymic activity	-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [ľmol/min/mg]	Specific Activity Maximum [ľmol/min/mg]	Commentary	Organism
0.149	-	pH 6.5, 22°C	Anopheles dirus

Substrates and Products (Substrate)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane	the reaction requires the presence of GSH	651503	Anopheles dirus	1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl	-	651503	Anopheles dirus	?

Inhibitors (protein specific)

Inhibitors	Commentary	Organism	Structure
bromosulfophthalein	-	Anopheles dirus
Ethacrynic acid	-	Anopheles dirus

KM Value [mM] (protein specific)

KM Value [mM]	KM Value Maximum [mM]	Substrate	Commentary	Organism	Structure
0.21	-	1-chloro-2,4-dinitrobenzene	pH 6.5, 22°C	Anopheles dirus

Specific Activity [micromol/min/mg] (protein specific)

Specific Activity Minimum [ľmol/min/mg]	Specific Activity Maximum [ľmol/min/mg]	Commentary	Organism
0.149	-	pH 6.5, 22°C	Anopheles dirus

Substrates and Products (Substrate) (protein specific)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane	the reaction requires the presence of GSH	651503	Anopheles dirus	1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl	-	651503	Anopheles dirus	?

See also following references to EC number 4.5.1.1 (sorted by year of publication):

No.	1st author	Pub Med	title	organims	journal	volume	pages	year	Activating Compound	Application	Cloned(Commentary)	Crystallization (Commentary)	Engineering	General Stability	Inhibitors	KM Value [mM]	Localization	Metals/Ions	Molecular Weight [Da]	Natural Substrates/ Products (Substrates)	Organic Solvent Stability	Organism	Oxidation Stability	Posttranslational Modification	Purification (Commentary)	Reaction	Renatured (Commentary)	Source Tissue	Specific Activity [micromol/min/mg]	Storage Stability	Substrates and Products (Substrate)	Subunits	Temperature Optimum [°C]	Temperature Range [°C]	Temperature Stability [°C]	Turnover Number [1/s]	pH Optimum	pH Range	pH Stability	Cofactor	Ki Value [mM]	pI Value	IC50 Value	Activating Compound (protein specific)	Application (protein specific)	Cloned(Commentary) (protein specific)	Cofactor (protein specific)	Crystallization (Commentary) (protein specific)	Engineering (protein specific)	General Stability (protein specific)	IC50 Value (protein specific)	Inhibitors (protein specific)	Ki Value [mM] (protein specific)	KM Value [mM] (protein specific)	Localization (protein specific)	Metals/Ions (protein specific)	Molecular Weight [Da] (protein specific)	Natural Substrates/ Products (Substrates) (protein specific)	Organic Solvent Stability (protein specific)	Oxidation Stability (protein specific)	Posttranslational Modification (protein specific)	Purification (Commentary) (protein specific)	Renatured (Commentary) (protein specific)	Source Tissue (protein specific)	Specific Activity [micromol/min/mg] (protein specific)	Storage Stability (protein specific)	Substrates and Products (Substrate) (protein specific)	Subunits (protein specific)	Temperature Optimum [°C] (protein specific)	Temperature Range [°C] (protein specific)	Temperature Stability [°C] (protein specific)	Turnover Number [1/s] (protein specific)	pH Optimum (protein specific)	pH Range (protein specific)	pH Stability (protein specific)	pI Value (protein specific)	Expression	General Information	General Information (protein specific)	Expression (protein specific)	KCat/KM [mM/s]	KCat/KM [mM/s] (protein specific)
714003	Latha	-	Effect of denaturants on the s ...	Pseudomonas putida	Asian J. Microbiol. Biotechnol. Environ. Sci.	13	51-57	2011	-	-	-	-	-	-	3	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
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649719	Ranson		Identification of a novel clas ...	Anopheles gambiae	Biochem. J.	359	295-304	2001	-	-	1	-	-	-	-	-	-	-	-	-	-	7	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
651503	Prapanthadara		Isoenzymes of glutathione S-tr ...	Anopheles dirus	Insect Biochem. Mol. Biol.	30	395-403	2000	-	-	-	-	-	-	2	1	-	-	-	-	-	4	-	-	-	-	-	-	1	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	2	-	1	-	-	-	-	-	-	-	-	-	-	1	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
653893	Prapanthadara	-	Correlation of glutathione S-t ...	Anopheles annularis, Anopheles minimus, Culex quinquefasciatus	Southeast Asian J. Trop. Med. Public Health	31	111-118	2000	-	3	-	-	-	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
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34742	Dinamarca		DDT-dehydrochlorinase. II. Sub ...	Musca domestica	Arch. Biochem. Biophys.	147	374-383	1971	1	-	-	-	-	-	1	-	-	-	-	1	-	2	-	1	-	-	-	-	-	-	2	2	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	1	-	-	-	-	-	1	-	-	1	-	-	-	-	-	2	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-
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34740	Lipke		DDT dehydrochlorinase. II. Sub ...	Musca domestica	J. Biol. Chem.	234	2129-2132	1959	1	-	-	-	-	-	1	-	-	7	-	1	-	1	-	-	-	-	-	-	-	-	8	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	1	-	-	-	7	-	1	-	-	-	-	-	-	-	-	8	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-