DDT dehydrochlorinase. I. Isolation, chemical properties, and spectrophotometric assay

Lipke, H.; Kearns, C.W.; J. Biol. Chem. 234, 2123-2128 (1959)

Data extracted from this reference:

Activating Compound

Activating Compound	Commentary	Organism	Structure
GSH	Km: 0.25 mM; required	Musca domestica

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Commentary	Organism
120000	-	PAGE	Musca domestica

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Commentary (Nat. Sub.)	Natural Products	Commentary (Nat. Pro.)	Organism (Nat. Pro.)	Reversibility
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane	Musca domestica	-	?	-	-	-

Organic Solvent Stability

Organic Solvent	Commentary	Organism
organic solvents	instability of the enzyme to organic solvents at temperatures about -5°C	Musca domestica

Organism

Organism	Primary Accession No. (UniProt)	Commentary	Textmining
Musca domestica	-	DDT-restistant housedly	-

Purification (Commentary)

Commentary	Organism
-	Musca domestica

Specific Activity [micromol/min/mg]

Specific Activity Minimum [ľmol/min/mg]	Specific Activity Maximum [ľmol/min/mg]	Commentary	Organism
additional information	-	-	Musca domestica

Storage Stability

Storage Stability	Organism
instability of the enzyme to freezing and thawing	Musca domestica

Substrates and Products (Substrate)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane	i.e. 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane, i.e. DDT in the presence of GSH	34739	Musca domestica	1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl	i.e. 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene i.e. DDE	34739	Musca domestica	ir
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane	-	34739	Musca domestica	?	-	-	-	-

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Commentary	Organism	Structure
0.0383	-	1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane	-	Musca domestica

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Commentary	Organism
7.4	-	-	Musca domestica

pH Stability

pH Stability	pH Stability Maximum	Commentary	Organism
4	6	stable between for 6 h	Musca domestica
6	12	stable between for 48 h at 2°C	Musca domestica

Activating Compound (protein specific)

Activating Compound	Commentary	Organism	Structure
GSH	Km: 0.25 mM; required	Musca domestica

Molecular Weight [Da] (protein specific)

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Commentary	Organism
120000	-	PAGE	Musca domestica

Natural Substrates/ Products (Substrates) (protein specific)

Natural Substrates	Organism	Commentary (Nat. Sub.)	Natural Products	Commentary (Nat. Pro.)	Organism (Nat. Pro.)	Reversibility
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane	Musca domestica	-	?	-	-	-

Organic Solvent Stability (protein specific)

Organic Solvent	Commentary	Organism
organic solvents	instability of the enzyme to organic solvents at temperatures about -5°C	Musca domestica

Purification (Commentary) (protein specific)

Commentary	Organism
-	Musca domestica

Specific Activity [micromol/min/mg] (protein specific)

Specific Activity Minimum [ľmol/min/mg]	Specific Activity Maximum [ľmol/min/mg]	Commentary	Organism
additional information	-	-	Musca domestica

Storage Stability (protein specific)

Storage Stability	Organism
instability of the enzyme to freezing and thawing	Musca domestica

Substrates and Products (Substrate) (protein specific)

Substrates	Commentary Substrates	Literature (Substrates)	Organism	Products	Commentary (Products)	Literature (Products)	Organism (Products)	Reversibility
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane	i.e. 1,1,1-trichloro-2,2-bis(p-chlorophenyl)ethane, i.e. DDT in the presence of GSH	34739	Musca domestica	1,1-dichloro-2,2-bis(4-chlorophenyl)ethylene + HCl	i.e. 1,1-dichloro-2,2-bis(p-chlorophenyl)ethylene i.e. DDE	34739	Musca domestica	ir
1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane	-	34739	Musca domestica	?	-	-	-	-

Turnover Number [1/s] (protein specific)

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Commentary	Organism	Structure
0.0383	-	1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane	-	Musca domestica

pH Optimum (protein specific)

pH Optimum Minimum	pH Optimum Maximum	Commentary	Organism
7.4	-	-	Musca domestica

pH Stability (protein specific)

pH Stability	pH Stability Maximum	Commentary	Organism
4	6	stable between for 6 h	Musca domestica
6	12	stable between for 48 h at 2°C	Musca domestica

See also following references to EC number 4.5.1.1 (sorted by year of publication):

No.	1st author	Pub Med	title	organims	journal	volume	pages	year	Activating Compound	Application	Cloned(Commentary)	Crystallization (Commentary)	Engineering	General Stability	Inhibitors	KM Value [mM]	Localization	Metals/Ions	Molecular Weight [Da]	Natural Substrates/ Products (Substrates)	Organic Solvent Stability	Organism	Oxidation Stability	Posttranslational Modification	Purification (Commentary)	Reaction	Renatured (Commentary)	Source Tissue	Specific Activity [micromol/min/mg]	Storage Stability	Substrates and Products (Substrate)	Subunits	Temperature Optimum [°C]	Temperature Range [°C]	Temperature Stability [°C]	Turnover Number [1/s]	pH Optimum	pH Range	pH Stability	Cofactor	Ki Value [mM]	pI Value	IC50 Value	Activating Compound (protein specific)	Application (protein specific)	Cloned(Commentary) (protein specific)	Cofactor (protein specific)	Crystallization (Commentary) (protein specific)	Engineering (protein specific)	General Stability (protein specific)	IC50 Value (protein specific)	Inhibitors (protein specific)	Ki Value [mM] (protein specific)	KM Value [mM] (protein specific)	Localization (protein specific)	Metals/Ions (protein specific)	Molecular Weight [Da] (protein specific)	Natural Substrates/ Products (Substrates) (protein specific)	Organic Solvent Stability (protein specific)	Oxidation Stability (protein specific)	Posttranslational Modification (protein specific)	Purification (Commentary) (protein specific)	Renatured (Commentary) (protein specific)	Source Tissue (protein specific)	Specific Activity [micromol/min/mg] (protein specific)	Storage Stability (protein specific)	Substrates and Products (Substrate) (protein specific)	Subunits (protein specific)	Temperature Optimum [°C] (protein specific)	Temperature Range [°C] (protein specific)	Temperature Stability [°C] (protein specific)	Turnover Number [1/s] (protein specific)	pH Optimum (protein specific)	pH Range (protein specific)	pH Stability (protein specific)	pI Value (protein specific)	Expression	General Information	General Information (protein specific)	Expression (protein specific)	KCat/KM [mM/s]	KCat/KM [mM/s] (protein specific)
714003	Latha	-	Effect of denaturants on the s ...	Pseudomonas putida	Asian J. Microbiol. Biotechnol. Environ. Sci.	13	51-57	2011	-	-	-	-	-	-	3	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
715180	Lumjuan		The role of the Aedes aegypti ...	Aedes aegypti	Insect Biochem. Mol. Biol.	41	203-209	2011	-	-	-	-	-	-	1	-	-	-	-	-	-	2	-	-	-	-	-	3	-	-	1	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	1	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	3	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
649719	Ranson		Identification of a novel clas ...	Anopheles gambiae	Biochem. J.	359	295-304	2001	-	-	1	-	-	-	-	-	-	-	-	-	-	7	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
651503	Prapanthadara		Isoenzymes of glutathione S-tr ...	Anopheles dirus	Insect Biochem. Mol. Biol.	30	395-403	2000	-	-	-	-	-	-	2	1	-	-	-	-	-	4	-	-	-	-	-	-	1	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	2	-	1	-	-	-	-	-	-	-	-	-	-	1	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
653893	Prapanthadara	-	Correlation of glutathione S-t ...	Anopheles annularis, Anopheles minimus, Culex quinquefasciatus	Southeast Asian J. Trop. Med. Public Health	31	111-118	2000	-	3	-	-	-	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	3	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
34744	Chang		Microbial succession and intes ...	Rattus norvegicus	J. Appl. Bacteriol.	77	709-718	1994	-	-	-	-	-	-	-	-	-	-	-	1	-	2	-	-	-	-	-	3	1	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	3	1	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
34743	Chadwick	-	Effect of lindane on intestina ...	Rattus norvegicus	Pestic. Biochem. Physiol.	38	48-56	1990	-	-	-	-	-	-	-	-	-	-	-	1	-	1	-	-	-	-	-	2	1	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	2	1	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
34742	Dinamarca		DDT-dehydrochlorinase. II. Sub ...	Musca domestica	Arch. Biochem. Biophys.	147	374-383	1971	1	-	-	-	-	-	1	-	-	-	-	1	-	2	-	1	-	-	-	-	-	-	2	2	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	1	-	-	-	-	-	1	-	-	1	-	-	-	-	-	2	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-
34741	Ishida	-	Comparative studies on BHC met ...	Musca domestica	Agric. Biol. Chem.	32	947-955	1968	1	-	-	-	-	-	-	-	-	-	-	1	-	2	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	-	2	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-
34739	Lipke		DDT dehydrochlorinase. I. Isol ...	Musca domestica	J. Biol. Chem.	234	2123-2128	1959	1	-	-	-	-	-	-	-	-	-	1	1	1	1	-	-	1	-	-	-	1	1	2	-	-	-	-	1	1	-	2	-	-	-	-	1	-	-	-	-	-	-	-	-	-	-	-	-	1	1	1	-	-	1	-	-	1	1	2	-	-	-	-	1	1	-	2	-	-	-	-	-	-	-
34740	Lipke		DDT dehydrochlorinase. II. Sub ...	Musca domestica	J. Biol. Chem.	234	2129-2132	1959	1	-	-	-	-	-	1	-	-	7	-	1	-	1	-	-	-	-	-	-	-	-	8	-	-	-	-	-	-	-	-	-	-	-	-	1	-	-	-	-	-	-	-	1	-	-	-	7	-	1	-	-	-	-	-	-	-	-	8	-	-	-	-	-	-	-	-	-	-	-	-	-	-	-