Purification of tryptophan synthase from Saccharomyces cerevisiae and partial activity of its nicked subunits

Bartholmes, P.; Boeker, H.; Jaenicke, R.; Eur. J. Biochem. 102, 167-172 (1979)

show all sequences of 4.2.1.20

Data extracted from this reference:

KM Value [mM]
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
0.07
-
Indole cosubstrate L-Ser Saccharomyces cerevisiae
0.17
-
Indole cosubstrate D-glyceraldehyde 3-phosphate Saccharomyces cerevisiae
1.1
-
D-Glyceraldehyde 3-phosphate cosubstrate L-Trp, i.e. reverse reaction Saccharomyces cerevisiae
Molecular Weight [Da]
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
153000
-
sedimentation equilibrium centrifugation Saccharomyces cerevisiae
Organism
Organism Primary Accession No. (UniProt) Commentary Textmining
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
Commentary Organism
-
Saccharomyces cerevisiae
Storage Stability
Storage Stability Organism
-18°C Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
1-(indol-3-yl)glycerol 3-phosphate + L-serine r 5555 Saccharomyces cerevisiae L-tryptophan + D-glyceraldehyde 3-phosphate + H2O
-
5555 Saccharomyces cerevisiae
-
indole + D-glyceraldehyde 3-phosphate r 5555 Saccharomyces cerevisiae indole-3-glycerol phosphate r 5555 Saccharomyces cerevisiae
-
indole + L-serine
-
5555 Saccharomyces cerevisiae L-tryptophan + H2O
-
5555 Saccharomyces cerevisiae
-
indole-3-glycerol phosphate r 5555 Saccharomyces cerevisiae indole + D-glyceraldehyde 3-phosphate r 5555 Saccharomyces cerevisiae
-
L-tryptophan + D-glyceraldehyde 3-phosphate + H2O r 5555 Saccharomyces cerevisiae indole-3-glycerol phosphate + L-serine r 5555 Saccharomyces cerevisiae
-
Subunits
Subunits Commentary Organism
dimer 2 * 77000, sedimentation equilibrium in 6 M guanidine hydrochloride Saccharomyces cerevisiae
pH Optimum
pH Optimum Minimum pH Optimum Maximum Commentary Organism
7.2
-
-
Saccharomyces cerevisiae
Cofactor
Cofactor Commentary Organism Structure
pyridoxal 5'-phosphate Km: 0.18 mM Saccharomyces cerevisiae
Cofactor (protein specific)
Cofactor Commentary Organism Structure
pyridoxal 5'-phosphate Km: 0.18 mM Saccharomyces cerevisiae
KM Value [mM] (protein specific)
KM Value [mM] KM Value Maximum [mM] Substrate Commentary Organism Structure
0.07
-
Indole cosubstrate L-Ser Saccharomyces cerevisiae
0.17
-
Indole cosubstrate D-glyceraldehyde 3-phosphate Saccharomyces cerevisiae
1.1
-
D-Glyceraldehyde 3-phosphate cosubstrate L-Trp, i.e. reverse reaction Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
Molecular Weight [Da] Molecular Weight Maximum [Da] Commentary Organism
153000
-
sedimentation equilibrium centrifugation Saccharomyces cerevisiae
Purification (Commentary) (protein specific)
Commentary Organism
-
Saccharomyces cerevisiae
Storage Stability (protein specific)
Storage Stability Organism
-18°C Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates Commentary Substrates Literature (Substrates) Organism Products Commentary (Products) Literature (Products) Organism (Products) Reversibility
1-(indol-3-yl)glycerol 3-phosphate + L-serine r 5555 Saccharomyces cerevisiae L-tryptophan + D-glyceraldehyde 3-phosphate + H2O
-
5555 Saccharomyces cerevisiae
-
indole + D-glyceraldehyde 3-phosphate r 5555 Saccharomyces cerevisiae indole-3-glycerol phosphate r 5555 Saccharomyces cerevisiae
-
indole + L-serine
-
5555 Saccharomyces cerevisiae L-tryptophan + H2O
-
5555 Saccharomyces cerevisiae
-
indole-3-glycerol phosphate r 5555 Saccharomyces cerevisiae indole + D-glyceraldehyde 3-phosphate r 5555 Saccharomyces cerevisiae
-
L-tryptophan + D-glyceraldehyde 3-phosphate + H2O r 5555 Saccharomyces cerevisiae indole-3-glycerol phosphate + L-serine r 5555 Saccharomyces cerevisiae
-
Subunits (protein specific)
Subunits Commentary Organism
dimer 2 * 77000, sedimentation equilibrium in 6 M guanidine hydrochloride Saccharomyces cerevisiae
pH Optimum (protein specific)
pH Optimum Minimum pH Optimum Maximum Commentary Organism
7.2
-
-
Saccharomyces cerevisiae


See also following references to EC number 4.2.1.20 (sorted by year of publication):
No.1st authorPub
Med
titleorganimsjournalvolumepagesyearActivating CompoundApplicationCloned(Commentary)Crystallization (Commentary)EngineeringGeneral StabilityInhibitorsKM Value [mM]LocalizationMetals/IonsMolecular Weight [Da]Natural Substrates/ Products (Substrates)Organic Solvent StabilityOrganismOxidation StabilityPosttranslational ModificationPurification (Commentary)ReactionRenatured (Commentary)Source TissueSpecific Activity [micromol/min/mg]Storage StabilitySubstrates and Products (Substrate)SubunitsTemperature Optimum [°C]Temperature Range [°C]Temperature Stability [°C]Turnover Number [1/s]pH OptimumpH RangepH StabilityCofactorKi Value [mM]pI ValueIC50 ValueActivating Compound (protein specific)Application (protein specific)Cloned(Commentary) (protein specific)Cofactor (protein specific)Crystallization (Commentary) (protein specific)Engineering (protein specific)General Stability (protein specific)IC50 Value (protein specific)Inhibitors (protein specific)Ki Value [mM] (protein specific)KM Value [mM] (protein specific)Localization (protein specific)Metals/Ions (protein specific)Molecular Weight [Da] (protein specific)Natural Substrates/ Products (Substrates) (protein specific)Organic Solvent Stability (protein specific)Oxidation Stability (protein specific)Posttranslational Modification (protein specific)Purification (Commentary) (protein specific)Renatured (Commentary) (protein specific)Source Tissue (protein specific)Specific Activity [micromol/min/mg] (protein specific)Storage Stability (protein specific)Substrates and Products (Substrate) (protein specific)Subunits (protein specific)Temperature Optimum [°C] (protein specific)Temperature Range [°C] (protein specific)Temperature Stability [°C] (protein specific)Turnover Number [1/s] (protein specific)pH Optimum (protein specific)pH Range (protein specific)pH Stability (protein specific)pI Value (protein specific)ExpressionGeneral InformationGeneral Information (protein specific)Expression (protein specific)KCat/KM [mM/s]KCat/KM [mM/s] (protein specific)
714502ZhaoEnzymatic synthesis of L-trypt ...Escherichia coliBiores. Technol.1023554-35572011111------1---1--------1-1---1------111---------1----------1-1---1---------
715294LaiX-ray and NMR crystallography ...Salmonella entericaJ. Am. Chem. Soc.1334-72011-----------1-2---1----1--------1------1----------1--------1---------------
701708PhillipsEffects of hydrostatic pressur ...Salmonella enterica subsp. enterica serovar TyphimuriumAnn. N. Y. Acad. Sci.118995-1032010----2--------2---1----------------------2---------------------------------
703681NishioLarge conformational changes i ...Escherichia coliFEBS J.2772157-21702010---1---------3---------1-------1------11-------------------1--------------
714182EhrmannLigand-induced formation of a ...Sulfolobus solfataricusBiochemistry4910842-108532010--1-4-----12-3---1----21-------------1--4-------12--------21---------22---
716453YinPlants contain two distinct cl ...Arabidopsis thaliana, Zea maysPhytochemistry711667-16722010--2-1--4-----8--2--2--2-2--42--2-----22-1----4-------2-2--2-2--42----11---
716660FatmiThe role of oligomerization an ...Salmonella enterica subsp. enterica serovar TyphimuriumPLoS Comput. Biol.6e10009942010-------------2---------1-----------------------------------1---------11---
701445ShenCharacterization of the putati ...Mycobacterium tuberculosisActa Biochim. Biophys. Sin.41379-3882009--1-8--18-22--3--1-----111-1181--1-----11-8----18-22----1----111-1181----11---
702257DierkersTryptophan synthase: structure ...Salmonella enterica subsp. enterica serovar TyphimuriumBiochemistry4810997-110102009---------4---2--------2------------------------4----------2---------------
706809BattilanaThe 1-deoxy-D: -xylulose 5-pho ...Vitis vinifera x Vitis riparia, Vitis vinifera x Vitis viniferaTheor. Appl. Genet.118653-6692009-------------2-------------------------------------------------------22---
677838PhillipsQuantitative effects of allost ...Salmonella enterica subsp. enterica serovar TyphimuriumArch. Biochem. Biophys.4708-192008--1-2----6---2--1-----1--------1-----11-2------6-----1----1---------------
692750PhillipsPressure and temperature jump ...Salmonella enterica subsp. enterica serovar TyphimuriumJ. Am. Chem. Soc.13013580-135882008--1--------1-2--1-----1--------------1-----------1---1----1---------------
693632VadrevuNMR analysis of partially fold ...Escherichia coliJ. Mol. Biol.377294-3062008--1-1--------3--1------1-------------1--1------------1-----1--------------
694034ShenHigh-cell-density regulation o ...Pseudomonas aeruginosaMicrobiology1542195-22082008-----------2-5--------3--------------------------2--------3---------------
694641SanjayaOverexpression of Arabidopsis ...Arabidopsis thalianaPlant Cell Environ.311074-10852008--1-1----1---7-----------------------1--1------1--------------------------
694748SalviniAlpha-tryptophan synthase of I ...Isatis tinctoriaPlant Physiol. Biochem.46715-7232008--1--------1-7-----2--1--------------1-----------1-----2--1---------------
678240RaboniControl of ionizable residues ...Salmonella enterica subsp. enterica serovar TyphimuriumBiochemistry4613223-132342007-------11-3---2--------1----12-1-1------1------11-3----------1----12-1--------
678297NgoSynthesis and characterization ...Salmonella enterica subsp. enterica serovar TyphimuriumBiochemistry467713-77272007---1---------2--------11-------1------11------------------11--------------
678298NgoAllosteric regulation of subst ...Salmonella enterica subsp. enterica serovar TyphimuriumBiochemistry467740-77532007---1-----2---2--1-----1--------1------11-------2-----1----1---------------
681414WuA tightly packed hydrophobic c ...Escherichia coliJ. Mol. Biol.3661624-16382007--1-10--------4--1-----1--------1-----11-10------------1----1---------------
682470HsiaoPlant native tryptophan syntha ...Arabidopsis thalianaPlanta225897-9062007-------------5-----1--1--------1------1----------------1--1---------------
682732YangLong-range side-chain-main-cha ...Salmonella enterica subsp. enterica serovar TyphimuriumProtein Sci.161398-14092007--1-3-----1--1--1-----1--------------1--3-------1----1----1---------------
678168LeopoldsederEvolution of multi-enzyme comp ...Sulfolobus solfataricusBiochemistry4514111-141192006----------1--3--------11-------1------1---------1---------11--------------
678399SpyrakisAllosteric communication betwe ...Salmonella enterica subsp. enterica serovar TyphimuriumBiochim. Biophys. Acta17641102-11092006----4--------2--------1--------1------1-4-----------------1---------------
679080DiasMolecular models of tryptophan ...Mycobacterium tuberculosisCell Biochem. Biophys.44375-3842006------6------3--------1--------1------1----6--------------1---------------
679191Goss-A convenient enzymatic synthes ...Salmonella entericaChem. Commun. (Camb.)474924-49252006--1--------1-1--------16--------1-----11----------1--------16---------------
680462AkersMolecular mechanism of tryptop ...Chlamydia trachomatisJ. Bacteriol.1884236-42432006-------------5--------1--------1------1-------------------1---------------
681986CarlsonIn vivo and in vitro studies o ...Chlamydia trachomatisMol. Microbiol.591678-16912006--1----------3--1-----1--------1-----11--------------1----1---------------
691756GossA convenient enzymatic synthes ...Salmonella entericaChem. Commun. (Camb.)20064924-49252006--1--------1-1-----1--18--------------1-----------1-----1--18---------------
664057LeeConformational changes in the ...Pyrococcus furiosusBiochemistry4411417-114272005---1---------3-------------------------1----------------------------------
664063NishioConformational changes in the ...Escherichia coliBiochemistry441184-11922005---1---------3-------------------------1----------------------------------
664115PhillipsHydrostatic pressure affects t ...Salmonella enterica subsp. enterica serovar TyphimuriumBiochemistry447921-792820051------------2---------------------1--------------------------------------
664952PioselliConfinement and crowding effec ...Salmonella enterica subsp. enterica serovar TyphimuriumFEBS Lett.5792197-22022005-------6-----2--------2----6-----------------6------------2----6----------
665429AsadaStabilization mechanism of the ...Thermus thermophilusJ. Biochem.138343-3532005--11---------4--1--------------------1-1-------------1--------------------
666043WintrodeAn obligatory intermediate con ...Escherichia coliJ. Mol. Biol.347911-9192005-------------3----1-----------------------------------1-------------------
666051WuSpecific structure appears at ...Escherichia coliJ. Mol. Biol.351445-4522005-----1-------3----1----------------------1------------1-------------------
666055KulikOn the structural basis of the ...Salmonella enterica subsp. enterica serovar TyphimuriumJ. Mol. Biol.352608-6202005---1---------3--------1----------------1------------------1---------------
666357JeongCrystallization and X-ray crys ...Escherichia coliMol. Cells19219-2222005--111--------3-----------------------1-11---------------------------------
649237JeongCrystallization and preliminar ...Escherichia coliActa Crystallogr. Sect. D60132-1342004--11---------3--11----22-------1-----111-------------1----22--------------
649777NibeilliuThe stereospecificity and cata ...Salmonella enterica subsp. enterica serovar TyphimuriumBiochem. J.381847-8522004--1----1-----1--11----21----1--------1-------1-------1----21----1---------
652550SchiarettipH dependence of tryptophan sy ...Salmonella enterica subsp. enterica serovar TyphimuriumJ. Biol. Chem.27929572-295822004-------1-1---2---1----211----2-1------1------1-1----------211----2--------
663845JeongStructures of wild-type and P2 ...Escherichia coliBiochem. Biophys. Res. Commun.3231257-12642004---11--------3--1----------------------11------------1--------------------
664824HiokiThe crystal structure of the t ...Pyrococcus furiosusEur. J. Biochem.2712624-26352004---1---------3-------------------------1----------------------------------
649608JeongFluorescence and folding prope ...Escherichia coliBiochem. Biophys. Res. Commun.30029-352003----2--------3---11---2-----------------2-------------1---2---------------
650222FerrariAllosteric communication in th ...Salmonella enterica subsp. enterica serovar TyphimuriumBiochemistry427807-781820033---2-11-----2---1----421--91--1---3--1-2--1-1------------421--91---------
650403RaboniThe molecular pathway for the ...Salmonella enterica subsp. enterica serovar TyphimuriumBiochim. Biophys. Acta1647157-1602003--112-1------2---1--7-32-------1-----1112--1------------7-32--------------
652475OsborneDetection of Open and Closed C ...Escherichia coliJ. Biol. Chem.27844083-440902003----3--------1---1----31-------1------1-3-----------------31--------------
652506OgasaharaStimulated interaction between ...Pyrococcus furiosusJ. Biol. Chem.2788922-89282003--1----1--3--4--11----24--1----------1-------1--3----1----24--1-----------
653803VadrevuPartial NMR assignments and se ...Escherichia coliProtein Sci.12185-1912003--1----------3--111---13-------------1---------------11---13--------------
650181HurProton transfers in the beta-r ...Salmonella enterica subsp. enterica serovar TyphimuriumBiochemistry419991-100012002------11-1---3---1----21-------1------1----1-1-1----------21--------------
652224WeyandCrystal structures of a new cl ...Salmonella enterica subsp. enterica serovar TyphimuriumJ. Biol. Chem.27710647-106522002---1--3------2---1----3----------------1---3--------------3---------------
652353HettwerA novel tryptophan synthase be ...Thermotoga maritimaJ. Biol. Chem.2778194-82012002--1----7--4--4--11----342--61--------1-------7--4----1----342--61---------
652899KulikOn the role of alphaThr183 in ...Salmonella enterica subsp. enterica serovar TyphimuriumJ. Mol. Biol.324677-6902002---11-1------2---1--1-41---14---1------111--1------------1-41---14----------
649580KimHomodimers of mutant tryptopha ...Escherichia coliBiochem. Biophys. Res. Commun.289568-5722001--1-6--1-----3--111-1-22-------------1--6----1-------11-1-22--------------
650053Weber-BanInvestigation of allosteric li ...Salmonella enterica subsp. enterica serovar TyphimuriumBiochemistry403497-35112001----3-41-4---2---1----32----------------3--4-1-4----------32--------------
650081FerrariBetaD305A mutant of tryptophan ...Salmonella enterica subsp. enterica serovar TyphimuriumBiochemistry407421-74322001----1-31-4---2---1----411--161--1------1-1--3-1-4----------411--161---------
650850MilesTryptophan synthase: a multien ...Salmonella enterica subsp. enterica serovar TyphimuriumChem. Rec.1140-1512001-------1---2-2---1----31-------1------1------1---2--------31--------------
652098YamagataEntropic stabilization of the ...Pyrococcus furiosusJ. Biol. Chem.27611062-110712001--11---------3--11----2---1----------1-1-------------1----2---1-----------
652118MarabottiAllosteric communication of tr ...Salmonella enterica subsp. enterica serovar TyphimuriumJ. Biol. Chem.27617747-177532001-1111--------2--11----31-------1----11111------------1----31--------------
649979FanRegulation of tryptophan synth ...Salmonella enterica subsp. enterica serovar TyphimuriumBiochemistry394692-470320001------1-2---2---1--3-32-1--1--1---1--1------1-2--------3-32-1--1---------
650304MarabottiNovel allosteric effectors of ...Salmonella enterica subsp. enterica serovar TyphimuriumBiochim. Biophys. Acta1476287-29920001-----8------2---1----311---1--18--1--1----88-------------311---1---------
650441RondardMutational scanning of a hairp ...Escherichia coli, Salmonella enterica subsp. enterica serovar TyphimuriumBiol. Chem.3811185-11932000----38--------4---2--2-642---2--2------2-38---------------2-642---2---------
651083TangBiochemical analysis of a ther ...Thermococcus kodakarensisEur. J. Biochem.2676369-63772000--1----------4--11--4-33321-2--------1---------------1--4-33321-2---------
652011FanThermal repair of tryptophan s ...Salmonella enterica subsp. enterica serovar TyphimuriumJ. Biol. Chem.27520302-203072000----2-2--2---2---1--6-2211--------------2--2---2--------6-2211------------
652070WeyandStructural basis for the impai ...Salmonella enterica subsp. enterica serovar TyphimuriumJ. Biol. Chem.27541058-410632000---11--1-----1---1----31---------------11----1------------31--------------
652086MozzarelliEffect of pH and monovalent ca ...Salmonella enterica subsp. enterica serovar TyphimuriumJ. Biol. Chem.2756956-69622000--11---1-2---2--11--1-211---1--1-----111-----1-2-----1--1-211---1---------
649852ZitzewitzMolecular dissection of the fo ...Escherichia coliBiochemistry3810205-102141999--1----------3--111---21--1----------1---------------11---21--1-----------
649865SachpatzidisCrystallographic studies of ph ...Salmonella enterica subsp. enterica serovar TyphimuriumBiochemistry3812665-126741999-1-1--6----2-1---1----2---------1---1--1---61----2--------2---------------
649871GualfettiApparent radii of the native, ...Escherichia coliBiochemistry3813367-133781999----------1--3---1----21--1---------------------1---------21--1-----------
649884WeyandCrystal structure of wild-type ...Salmonella enterica subsp. enterica serovar TyphimuriumBiochemistry3816469-164801999---1---------1---1----3----------------1------------------3---------------
649894BaharCooperative fluctuations and s ...Salmonella enterica subsp. enterica serovar TyphimuriumBiochemistry383478-34901999-----------3-2---1----62-------------------------3--------62--------------
649898WoehlMechanisms of monovalent catio ...Salmonella enterica subsp. enterica serovar TyphimuriumBiochemistry387118-71301999-------1-----2---1----211---1--11-----1-----11------------211---1---------
649899WoehlMechanisms of monovalent catio ...Salmonella enterica subsp. enterica serovar TyphimuriumBiochemistry387131-71411999-------1-3---2---1----2-1---1--1------1------1-3----------2-1---1---------
649903FanGuanidine hydrochloride exerts ...Salmonella enterica subsp. enterica serovar TyphimuriumBiochemistry387881-789019991-1--11--1---2--11----621---1--1---1-11--1-1---1-----1----621---1---------
651955RoCatalytic mechanism of the try ...Salmonella enterica subsp. enterica serovar TyphimuriumJ. Biol. Chem.27431189-311941999------31-----2---1----21-------12-----1----321------------21--------------
651967RoStructure and function of the ...Salmonella enterica subsp. enterica serovar TyphimuriumJ. Biol. Chem.27436439-364451999----1--1-----2---1----111---2--1------1-1----1------------111---2---------
5571RekoslavskavaTryptophan synthase from Agrob ...Agrobacterium tumefaciensBiokhimiya62433-4391997-------------3--1----1-1--1--------------------------1---1-1--1-----------
5572RadwanskiCharacterization of tryptophan ...Arabidopsis thalianaMol. Gen. Genet.253353-3611996--1----------3-----1-----------------1-----------------1------------------
5574AhmedA thermally induced reversible ...Escherichia coliJ. Biol. Chem.2718612-86171996-------------2--------2--------1------1-------------------2---------------
5567MilesTryptophan synthase, structure ...Escherichia coli, Salmonella enterica subsp. enterica serovar TyphimuriumSubcell. Biochem.24207-2541995---1111-------2---2----92--2----2------21111----------------92--2-----------
5575ChoiTryptophan-containing alpha-su ...Escherichia coliJ. Biol. Chem.27017712-177151995----2--------3--------------------------2---------------------------------
5576PeracchiMonovalent cations affect dyna ...Salmonella enterica subsp. enterica serovar TyphimuriumBiochemistry349459-94651995-------5-----1--------1----5-----------------5------------1----5----------
5577ChoiUnfolding properties of trypto ...Escherichia coliJ. Biol. Chem.27028177-281821995----2--------3--------------------------2---------------------------------
5578AndersonKinetic characterization of ch ...Escherichia coliJ. Biol. Chem.27029936-299441995----1--------2--------------------------1---------------------------------
33302ZhaoImmunological characterization ...Arabidopsis thalianaJ. Biol. Chem.2706081-60871995--1-----1----1---------1-------------1--------1------------1--------------
715116Barczak5-Fluoroindole resistance iden ...Arabidopsis thalianaGenetics140303-3131995----1--------3--------2-----------------1-----------------2---------------
716584Zhao Guo PinSynechocystis PCC 6803 contain ...Synechocystis sp.Plant Physiol.104461-4661994--1----------8--------1--------------1--------------------1---------------
714514KurahashiCloning and nucleotide sequenc ...Bacillus subtilisBiosci. Biotechnol. Biochem.571006-10091993----------1--3----------------------------------1-------------------------
5579ZhaoGenetic and biochemical charac ...Escherichia coliJ. Biol. Chem.267526-5411992----1--------3--------------------------1---------------------------------
5580YangOverexpression and purificatio ...Salmonella enterica subsp. enterica serovar TyphimuriumProtein Expr. Purif.3347-3541992--1----------3--1----21--------------1---------------1---21---------------
5581Ki LimEnzymatic properties of mutant ...Escherichia coliJ. Biol. Chem.26620205-202121991----1--------3--------------------------1---------------------------------
5582Blond-ElguindiKinetic characterization of ea ...Escherichia coliBiochemistry292409-24171990-------------3----1-----------------------------------1-------------------
715413MozzarelliMicrospectrophotometric studie ...Salmonella enterica subsp. enterica serovar TyphimuriumJ. Biol. Chem.26415774-157801989---1---------1--1-----21-------1------11-------------1----21--------------
716794BerlynA gene encoding the tryptophan ...Arabidopsis thalianaProc. Natl. Acad. Sci. USA864604-46081989--1----------4--------2--------------1--------------------2---------------
5548PrattNeurospora tryptophan synthase ...Neurospora crassaJ. Biol. Chem.2636872-68761988-------------6--------3--------1------1-------------------3---------------
5568HydeThree-dimensional structure of ...Salmonella enterica subsp. enterica serovar TyphimuriumJ. Biol. Chem.26317857-178711988---1---------2--------3----------------1------------------3---------------
5549CrawfordPrediction of secondary struct ...Bacillus subtilis, Caulobacter vibrioides, Corynebacterium glutamicum, Enterobacter aerogenes, Escherichia coli, Lactobacillus casei, Neurospora crassa, Proteus mirabilis, Pseudomonas aeruginosa, Saccharomyces cerevisiae, Salmonella enterica subsp. enterica serovar Typhimurium, Serratia marcescens, Vibrio parahaemolyticusProteins Struct. Funct. Genet.2118-1291987-------------14---------10-----------------------------------10--------------
5550MilesTryptophan synthase from Esche ...Escherichia coli, Salmonella enterica subsp. enterica serovar TyphimuriumMethods Enzymol.142398-4141987------4------3--2---2-16--------2------2----4---------2--2-16---------------
5551YutaniDependence of conformational s ...Escherichia coliProc. Natl. Acad. Sci. USA844441-44441987----1--------3------------1-------------1---------------------1-----------
5569AhmedMicrocrystals of tryptophan sy ...Salmonella enterica subsp. enterica serovar TyphimuriumBiochemistry265492-54981987---1---5-----3--------4----5-----------1-----5------------4----5----------
5552MiltonIn vitro mutagensis and overex ...Escherichia coliJ. Biol. Chem.26116604-166151986--1----------3-----------------------1------------------------------------
5553DreweCharacterization of the reacti ...Escherichia coliBiochemistry252494-25011986-------------3--------1-----------------------------------1---------------
5570LaneThe mechanism of self-assembly ...Escherichia coliEMBO J.3279-2871984-------------3--------31----------------------------------31--------------
5554BaileyPurification and properties of ...Saccharomyces cerevisiaeBiochem. J.209151-1571983-------2-----2--1---1111---------------------2-------1--1111--------------
658339BangProduction of L-tryptophan by ...Escherichia coliBiotechnol.Bioeng.25999-10111983-------------4--------1-1----1-1------1-------------------1-1----1--------
5555BartholmesPurification of tryptophan syn ...Saccharomyces cerevisiaeEur. J. Biochem.102167-1721979-------3--1--2--1----151----1--1------1------3--1----1---151----1---------
5556RochaPurification and partial chara ...Serratia marcescensJ. Bacteriol.134950-9571978-------------2--1---1-11-------1------1--------------1--1-11--------------
5557RiverinPurification and properties of ...Proteus mirabilisJ. Biol. Chem.2513875-38801976-------------5--1-----7------------------------------1----7---------------
5558ShannonPurification and immunoadsorbt ...Escherichia coliEur. J. Biochem.63563-5681976-------------3--1---1-1------------------------------1--1-1---------------
5559KirschnerThe tryptophan synthase from E ...Escherichia coliEur. J. Biochem.60513-5231975------3------3--1---1-2---------3----------33--------1--1-2---------------
5560WolfTryptophan synthase from yeast ...Saccharomyces cerevisiaeEur. J. Biochem.45269-2761974-----194-----2--1----13-----1-1-5--------1-954-------1---13-----1-1-------
5561Owens-The purification and structure ...Neurospora crassaBiochem. Soc. Trans.21331-13321974-------------1--1------1-----------------------------1-----1--------------
5562Zaffaroni-Synthesis of L-tryptophan from ...Escherichia coliAgric. Biol. Chem.381335-13421974-------------1--1---111---1-1------------------------1--111---1-1---------
5563O'Neil HochTryptophan synthetase from Bac ...Bacillus subtilisJ. Biol. Chem.2482999-300319731------------2--1---1-11-----1-----1----